CmaCh05G002470.1 (mRNA) Cucurbita maxima (Rimu)

BLAST of CmaCh05G002470.1 vs. Swiss-Prot
Match: FAD25_MYCTU (Putative fatty-acid--CoA ligase fadD25 OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) GN=fadD25 PE=1 SV=1)

HSP 1 Score: 171.0 bits (432), Expect = 4.1e-41
Identity = 156/575 (27.13%), Postives = 254/575 (44.17%), Query Frame = 1

Query: 52  LTYGQLHDSVQFISEQLIRQVRRRDTVIVLCSPGLDLVQLIYGCQRAGLVSVPISPPDPC 111
           LT+ QL+     ++ QL       D  ++L    LD V       +AG+V+VP+S P   
Sbjct: 41  LTWSQLYRRTLNLAAQLREHGSTGDRALILAPQSLDYVVSFIASLQAGIVAVPLSIPQGG 100

Query: 112 FENENCHHFARALSQTKPRAVIAHEPYIVSVFRYLSSSQIDGKLALLLQSVQWISMETLR 171
             +E         + T P  V+     + +V  Y+      G+ A  +  V  + ++   
Sbjct: 101 AHDERT---VSVFADTAPAIVLTASSVVDNVVEYVQPQP--GQNAPAVIEVDRLDLDA-- 160

Query: 172 TCTAAEADQVVSVISSKDRPFFYHSNSSYYGCKPEEPYLIQYTSGATGIPKPVVVTAGAA 231
                             RP    S S+ +G  P+  YL QYTSG+T  P  V+V+    
Sbjct: 161 ------------------RPSS-GSRSAAHG-HPDILYL-QYTSGSTRTPAGVMVSNKNL 220

Query: 232 AHNVRAARKAYD------LNPNDVIVSWLPQYHDCGLMF-LLLTVVSGATCVLTSPISFV 291
             N      +Y         P   +VSWLP YHD G +  L+L +++G   VLTSPI F+
Sbjct: 221 FANFEQIMTSYYGVYGKVAPPGSTVVSWLPFYHDMGFVLGLILPILAGIPAVLTSPIGFL 280

Query: 292 TRPITWLHLITTFKATCTPVPSFTLPLVLKRVKEETPPGGDLDLCCLRNLILINEPVYRS 351
            RP  W+ ++ +     T  P+F   L  ++ K+E   G  LDL  +  ++  +E V   
Sbjct: 281 QRPARWIQMLASNTLAFTAAPNFAFDLASRKTKDEDMEG--LDLGGVHGILNGSERVQPV 340

Query: 352 GVEEFVDVFKAVGLNPGCVSPSYGLAENSTFVSTAWSGGEGGLRRCFPAMPSYRKLLPCA 411
            ++ F+D F    L+P  + PSYG+AE + +V+T  +G    + +  P            
Sbjct: 341 TLKRFIDRFAPFNLDPKAIRPSYGMAEATVYVATRKAGQPPKIVQFDP-----------Q 400

Query: 412 RLTDGSSAEIE-----------------VVVVNGETGEMVDDGVEGEIWISSPSNASGYL 471
           +L DG +   E                 V +V+ +TG     G  GEIW+   + A GY 
Sbjct: 401 KLPDGQAERTESDGGTPLVSYGIVDTQLVRIVDPDTGIERPAGTIGEIWVHGDNVAIGYW 460

Query: 472 GHPLLTRDTFHCKLSNKS----SHKFVRTGDRGVIKGADRFLFVIGRCSDVITLTNNQEI 531
             P  T  TF   + N S    +  ++RTGD G +   +  LF++GR  D++ +   +  
Sbjct: 461 QKPEATERTFSATIVNPSEGTPAGPWLRTGDSGFLSEGE--LFIMGRIKDLL-IVYGRNH 520

Query: 532 HPHYIESIAYNNFSAYLRGGCLAAIKVS----DTIAVVAEMQREDKND---AETLRQICE 591
            P  IE+         +  G  AAI VS    + +  + E++++D++D   AE L  +  
Sbjct: 521 SPDDIEATIQT-----ISPGRCAAIAVSEHGAEKLVAIIELKKKDESDDEAAERLGFVKR 566

BLAST of CmaCh05G002470.1 vs. Swiss-Prot
Match: FAD25_MYCTO (Putative fatty-acid--CoA ligase fadD25 OS=Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) GN=fadD25 PE=3 SV=1)

HSP 1 Score: 171.0 bits (432), Expect = 4.1e-41
Identity = 156/575 (27.13%), Postives = 254/575 (44.17%), Query Frame = 1

Query: 52  LTYGQLHDSVQFISEQLIRQVRRRDTVIVLCSPGLDLVQLIYGCQRAGLVSVPISPPDPC 111
           LT+ QL+     ++ QL       D  ++L    LD V       +AG+V+VP+S P   
Sbjct: 41  LTWSQLYRRTLNLAAQLREHGSTGDRALILAPQSLDYVVSFIASLQAGIVAVPLSIPQGG 100

Query: 112 FENENCHHFARALSQTKPRAVIAHEPYIVSVFRYLSSSQIDGKLALLLQSVQWISMETLR 171
             +E         + T P  V+     + +V  Y+      G+ A  +  V  + ++   
Sbjct: 101 AHDERT---VSVFADTAPAIVLTASSVVDNVVEYVQPQP--GQNAPAVIEVDRLDLDA-- 160

Query: 172 TCTAAEADQVVSVISSKDRPFFYHSNSSYYGCKPEEPYLIQYTSGATGIPKPVVVTAGAA 231
                             RP    S S+ +G  P+  YL QYTSG+T  P  V+V+    
Sbjct: 161 ------------------RPSS-GSRSAAHG-HPDILYL-QYTSGSTRTPAGVMVSNKNL 220

Query: 232 AHNVRAARKAYD------LNPNDVIVSWLPQYHDCGLMF-LLLTVVSGATCVLTSPISFV 291
             N      +Y         P   +VSWLP YHD G +  L+L +++G   VLTSPI F+
Sbjct: 221 FANFEQIMTSYYGVYGKVAPPGSTVVSWLPFYHDMGFVLGLILPILAGIPAVLTSPIGFL 280

Query: 292 TRPITWLHLITTFKATCTPVPSFTLPLVLKRVKEETPPGGDLDLCCLRNLILINEPVYRS 351
            RP  W+ ++ +     T  P+F   L  ++ K+E   G  LDL  +  ++  +E V   
Sbjct: 281 QRPARWIQMLASNTLAFTAAPNFAFDLASRKTKDEDMEG--LDLGGVHGILNGSERVQPV 340

Query: 352 GVEEFVDVFKAVGLNPGCVSPSYGLAENSTFVSTAWSGGEGGLRRCFPAMPSYRKLLPCA 411
            ++ F+D F    L+P  + PSYG+AE + +V+T  +G    + +  P            
Sbjct: 341 TLKRFIDRFAPFNLDPKAIRPSYGMAEATVYVATRKAGQPPKIVQFDP-----------Q 400

Query: 412 RLTDGSSAEIE-----------------VVVVNGETGEMVDDGVEGEIWISSPSNASGYL 471
           +L DG +   E                 V +V+ +TG     G  GEIW+   + A GY 
Sbjct: 401 KLPDGQAERTESDGGTPLVSYGIVDTQLVRIVDPDTGIERPAGTIGEIWVHGDNVAIGYW 460

Query: 472 GHPLLTRDTFHCKLSNKS----SHKFVRTGDRGVIKGADRFLFVIGRCSDVITLTNNQEI 531
             P  T  TF   + N S    +  ++RTGD G +   +  LF++GR  D++ +   +  
Sbjct: 461 QKPEATERTFSATIVNPSEGTPAGPWLRTGDSGFLSEGE--LFIMGRIKDLL-IVYGRNH 520

Query: 532 HPHYIESIAYNNFSAYLRGGCLAAIKVS----DTIAVVAEMQREDKND---AETLRQICE 591
            P  IE+         +  G  AAI VS    + +  + E++++D++D   AE L  +  
Sbjct: 521 SPDDIEATIQT-----ISPGRCAAIAVSEHGAEKLVAIIELKKKDESDDEAAERLGFVKR 566

BLAST of CmaCh05G002470.1 vs. Swiss-Prot
Match: FAA28_MYCMM (Long-chain-fatty-acid--AMP ligase FadD28 OS=Mycobacterium marinum (strain ATCC BAA-535 / M) GN=fadD28 PE=3 SV=1)

HSP 1 Score: 168.3 bits (425), Expect = 2.7e-40
Identity = 159/582 (27.32%), Postives = 251/582 (43.13%), Query Frame = 1

Query: 40  DGTADVLNEGSFLTYGQLHDSVQFISEQLIRQVRRRDTVIVLCSPGLDLVQLIYGCQRAG 99
           DG A+ L      T+ QL+     ++ +L       D  ++L   GL+ V    G  +AG
Sbjct: 33  DGAAETL------TWSQLYRRTLNVARELSSCGSVGDRAVILAPQGLEYVVAFLGALQAG 92

Query: 100 LVSVPISPPDPCFENENCHHFARALSQTKPRAVIAHEPYIVSVFRYLSSSQIDGKLALLL 159
            ++VP+S P     +E        L    P A++   P I  V +++S+           
Sbjct: 93  RIAVPLSVPQGGASDERA---TSVLRDASPVAILTTSPVIDDVTQHVSAQ---------- 152

Query: 160 QSVQWISMETLRTCTAAEADQVVSVISSKDRPFFYHSNSSYYGCK--PEEPYLIQYTSGA 219
                         +A  A  ++ +    DR     +  S  G +  P   YL QYTSG+
Sbjct: 153 --------------SAGPAPSIIEL----DRLDLDAAAGSGAGTENYPATAYL-QYTSGS 212

Query: 220 TGIPKPVVVTAGAAAHNVRAARKAYDLN------PNDVIVSWLPQYHDCGLMF-LLLTVV 279
           T  P  V+++      N       Y  +      P+  +VSWLP YHD GL+  +   ++
Sbjct: 213 TREPAGVMLSHQNLVTNFEQLMSGYFADTDGIAPPDSTLVSWLPFYHDMGLVLGVCAPIL 272

Query: 280 SGATCVLTSPISFVTRPITWLHLITTFKATCTPVPSFTLPLVLKRVKEETPPGGDLDLCC 339
            G   VLTSP+SF+ RP  WL ++ T     +  P+F   L  K+V ++   G  LDL  
Sbjct: 273 GGYQAVLTSPVSFLQRPARWLQMLATSSHAFSAAPNFAFELAAKKVSDDDMAG--LDLGN 332

Query: 340 LRNLILINEPVYRSGVEEFVDVFKAVGLNPGCVSPSYGLAENSTFVSTAWSGGEGGLRRC 399
           +  ++  +E V  + ++ F D F    L    + PSYGLAE + +VST+  G    L   
Sbjct: 333 VLTILSGSERVQPATLKRFADRFARFNLQEKVLRPSYGLAEATVYVSTSRPGQPPELVEF 392

Query: 400 FPAMPSYRKLLPCARLTDGSSAEIE---------VVVVNGETGEMVDDGVEGEIWISSPS 459
                S  +   C     G+  ++          V VV+ +T     DG  GEIW+   +
Sbjct: 393 DAESLSTGQAKQC---ESGAGTQLVSYVLPRSPIVRVVDPDTCTECPDGTVGEIWVCGDN 452

Query: 460 NASGYLGHPLLTRDTFHCKLSNKS----SHKFVRTGDRGVIKGADRFLFVIGRCSDVITL 519
            A GY   P  +  TF  KL++ S       ++RTGD G I   D  +F+IGR  D++ +
Sbjct: 453 VAIGYWNKPEESERTFGGKLASPSEGTPEGPWLRTGDSGFI--TDGKMFIIGRIKDLL-I 512

Query: 520 TNNQEIHPHYIESIAYNNFSAYLRGGCLAAIKV-----SDTIAVVAEMQREDKNDAETLR 579
              +   P  IE+          R  C AAI V     ++ +  + E +R   +D E + 
Sbjct: 513 VYGRNHSPDDIEA----TIQEITRSRC-AAISVPGDRSTEKLVAIIEYRRRGDSDQEAMD 563

Query: 580 QIC---EGIRKAALIEEGIELGLVVLVKRGNVPKTTSGKVKR 592
            +      +  A     G+ +  +VLV  G++P TTSGKV+R
Sbjct: 573 MLVAVKREVTSALSNSHGLSVADLVLVAPGSIPTTTSGKVRR 563

BLAST of CmaCh05G002470.1 vs. Swiss-Prot
Match: FAA28_MYCBO (Long-chain-fatty-acid--AMP ligase FadD28 OS=Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) GN=fadD28 PE=3 SV=2)

HSP 1 Score: 151.8 bits (382), Expect = 2.6e-35
Identity = 126/423 (29.79%), Postives = 196/423 (46.34%), Query Frame = 1

Query: 205 PEEPYLIQYTSGATGIPKPVVVTAGAAAHNVRAARKAYDLN------PNDVIVSWLPQYH 264
           P   YL QYTSG+T  P  VV++      N       Y  +      PN  +VSWLP YH
Sbjct: 163 PSTAYL-QYTSGSTRTPAGVVMSHQNVRVNFEQLMSGYFADTDGIPPPNSALVSWLPFYH 222

Query: 265 DCGLMF-LLLTVVSGATCVLTSPISFVTRPITWLHLITTFKATCTPVPSFTLPLVLKRVK 324
           D GL+  +   ++ G   VLTSP+SF+ RP  W+HL+ +     +  P+F   L  +R  
Sbjct: 223 DMGLVIGICAPILGGYPAVLTSPVSFLQRPARWMHLMASDFHAFSAAPNFAFELAARRTT 282

Query: 325 EETPPGGDLDLCCLRNLILI---NEPVYRSGVEEFVDVFKAVGLNPGCVSPSYGLAENST 384
           ++   G DL      N++ I   +E V  + ++ F D F    L    + PSYGLAE + 
Sbjct: 283 DDDMAGRDLG-----NILTILSGSERVQAATIKRFADRFARFNLQERVIRPSYGLAEATV 342

Query: 385 FVSTAWSGGEGGLRRCFPAMPSYRKLLPCARLTDGSSAEIE--------VVVVNGETGEM 444
           +V+T+  G             S     PCA    G+++ I         V +V+ +T   
Sbjct: 343 YVATSKPGQPPETVDFDTESLSAGHAKPCA--GGGATSLISYMLPRSPIVRIVDSDTCIE 402

Query: 445 VDDGVEGEIWISSPSNASGYLGHPLLTRDTFHCKLSNKS----SHKFVRTGDRGVIKGAD 504
             DG  GEIW+   + A+GY   P  +  TF  K+   S       ++RTGD G +   D
Sbjct: 403 CPDGTVGEIWVHGDNVANGYWQKPDESERTFGGKIVTPSPGTPEGPWLRTGDSGFV--TD 462

Query: 505 RFLFVIGRCSDVITLTNNQEIHPHYIESIAYNNFSAYLRGGCLAAIKV-----SDTIAVV 564
             +F+IGR  D++ +   +   P  IE+          RG C AAI V     ++ +  +
Sbjct: 463 GKMFIIGRIKDLL-IVYGRNHSPDDIEA----TIQEITRGRC-AAISVPGDRSTEKLVAI 522

Query: 565 AEMQREDKNDAETLRQ---ICEGIRKAALIEEGIELGLVVLVKRGNVPKTTSGKVKRWLA 598
            E+++   +D + + +   I   +  A     G+ +  +VLV  G++P TTSGKV+R   
Sbjct: 523 IELKKRGDSDQDAMARLGAIKREVTSALSSSHGLSVADLVLVAPGSIPITTSGKVRRGAC 569

BLAST of CmaCh05G002470.1 vs. Swiss-Prot
Match: FAA28_MYCTO (Long-chain-fatty-acid--AMP ligase FadD28 OS=Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) GN=fadD28 PE=3 SV=1)

HSP 1 Score: 151.8 bits (382), Expect = 2.6e-35
Identity = 126/423 (29.79%), Postives = 196/423 (46.34%), Query Frame = 1

Query: 205 PEEPYLIQYTSGATGIPKPVVVTAGAAAHNVRAARKAYDLN------PNDVIVSWLPQYH 264
           P   YL QYTSG+T  P  VV++      N       Y  +      PN  +VSWLP YH
Sbjct: 163 PSTAYL-QYTSGSTRTPAGVVMSHQNVRVNFEQLMSGYFADTDGIPPPNSALVSWLPFYH 222

Query: 265 DCGLMF-LLLTVVSGATCVLTSPISFVTRPITWLHLITTFKATCTPVPSFTLPLVLKRVK 324
           D GL+  +   ++ G   VLTSP+SF+ RP  W+HL+ +     +  P+F   L  +R  
Sbjct: 223 DMGLVIGICAPILGGYPAVLTSPVSFLQRPARWMHLMASDFHAFSAAPNFAFELAARRTT 282

Query: 325 EETPPGGDLDLCCLRNLILI---NEPVYRSGVEEFVDVFKAVGLNPGCVSPSYGLAENST 384
           ++   G DL      N++ I   +E V  + ++ F D F    L    + PSYGLAE + 
Sbjct: 283 DDDMAGRDLG-----NILTILSGSERVQAATIKRFADRFARFNLQERVIRPSYGLAEATV 342

Query: 385 FVSTAWSGGEGGLRRCFPAMPSYRKLLPCARLTDGSSAEIE--------VVVVNGETGEM 444
           +V+T+  G             S     PCA    G+++ I         V +V+ +T   
Sbjct: 343 YVATSKPGQPPETVDFDTESLSAGHAKPCA--GGGATSLISYMLPRSPIVRIVDSDTCIE 402

Query: 445 VDDGVEGEIWISSPSNASGYLGHPLLTRDTFHCKLSNKS----SHKFVRTGDRGVIKGAD 504
             DG  GEIW+   + A+GY   P  +  TF  K+   S       ++RTGD G +   D
Sbjct: 403 CPDGTVGEIWVHGDNVANGYWQKPDESERTFGGKIVTPSPGTPEGPWLRTGDSGFV--TD 462

Query: 505 RFLFVIGRCSDVITLTNNQEIHPHYIESIAYNNFSAYLRGGCLAAIKV-----SDTIAVV 564
             +F+IGR  D++ +   +   P  IE+          RG C AAI V     ++ +  +
Sbjct: 463 GKMFIIGRIKDLL-IVYGRNHSPDDIEA----TIQEITRGRC-AAISVPGDRSTEKLVAI 522

Query: 565 AEMQREDKNDAETLRQ---ICEGIRKAALIEEGIELGLVVLVKRGNVPKTTSGKVKRWLA 598
            E+++   +D + + +   I   +  A     G+ +  +VLV  G++P TTSGKV+R   
Sbjct: 523 IELKKRGDSDQDAMARLGAIKREVTSALSSSHGLSVADLVLVAPGSIPITTSGKVRRGAC 569

BLAST of CmaCh05G002470.1 vs. TrEMBL
Match: A0A0A0LL23_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G361690 PE=4 SV=1)

HSP 1 Score: 951.0 bits (2457), Expect = 7.1e-274
Identity = 483/635 (76.06%), Postives = 536/635 (84.41%), Query Frame = 1

Query: 1   MSYENFDPIFPDQPVVDLYLPVWASLPAFRSKPAFIWSEDGTADVLNEGSFLTYGQLHDS 60
           M YENFDP+FP+QPVVD YLPVWASLPAF+SKPAFIWSEDGTA  +NE SFLTY QLHDS
Sbjct: 1   MLYENFDPLFPEQPVVDRYLPVWASLPAFQSKPAFIWSEDGTAKAINEDSFLTYRQLHDS 60

Query: 61  VQFISEQLIRQVRRRDTVIVLCSPGLDLVQLIYGCQRAGLVSVPISPPDPCFENENCHHF 120
           VQ I+EQL+RQ+RRRDTV+VLCS GLD VQLIYGCQRAGLVSVPISPPD   ENENCHH 
Sbjct: 61  VQLITEQLLRQLRRRDTVVVLCSAGLDFVQLIYGCQRAGLVSVPISPPDVFSENENCHHL 120

Query: 121 ARALSQTKPRAVIAHEPYIVSVFRYLSSSQIDGKLALLLQSVQWISMETLRTCTAAEADQ 180
           ARALSQTKPRA IAH+ YI +VFRY+SSS  D KLALLL+ V+WISME+L+     +  +
Sbjct: 121 ARALSQTKPRAAIAHQSYINTVFRYISSSA-DKKLALLLKGVRWISMESLK-----QPHK 180

Query: 181 VVSVISSKDRPFFYHSNSSYYGCKPEEPYLIQYTSGATGIPKPVVVTAGAAAHNVRAARK 240
              +   K +  FYHS SSY GC P++PYLIQYTSGAT I K VV+TAGAAAHNVRAARK
Sbjct: 181 ESELNQHKHQSSFYHS-SSYSGCNPDDPYLIQYTSGATAISKAVVITAGAAAHNVRAARK 240

Query: 241 AYDLNPNDVIVSWLPQYHDCGLMFLLLTVVSGATCVLTSPISFVTRPITWLHLITTFKAT 300
           AYDLNPND+IVSWLPQYHDCGLMFLLLTV++GATCVLTSPISFVT+PITWLHLIT FKAT
Sbjct: 241 AYDLNPNDLIVSWLPQYHDCGLMFLLLTVITGATCVLTSPISFVTQPITWLHLITAFKAT 300

Query: 301 CTPVPSFTLPLVLKRVKEETPPGGDLDLCCLRNLILINEPVYRSGVEEFVDVFKAVGLNP 360
           CTPVPSFTLPLVLKRV   +   G+LDL  LRNLILINEP+YRS VEEFV+VFK VGL+P
Sbjct: 301 CTPVPSFTLPLVLKRVGYSSGRAGNLDLRSLRNLILINEPIYRSTVEEFVEVFKTVGLDP 360

Query: 361 GCVSPSYGLAENSTFVSTAWSGGEGGLRRCFPAMPSYRKLLPCARLTDGSSAEIEVVVVN 420
           GCVSPSYGLAEN TFVSTAW GG G     FPAMPSYR+LLP  RL DG   EIEVVVVN
Sbjct: 361 GCVSPSYGLAENCTFVSTAWCGGGG-----FPAMPSYRQLLPSGRLRDGMCKEIEVVVVN 420

Query: 421 GETGEMVDDGVEGEIWISSPSNASGYLGHPLLTRDTFHCKLSNKSSHKFVRTGDRGVIKG 480
            ETGE+V+DGVEGEIW+SSPSNA GYLGHP +T +TFH KL NKSS  FVRTGDRGVIKG
Sbjct: 421 EETGEVVEDGVEGEIWVSSPSNALGYLGHPSVTEETFHSKLKNKSSLNFVRTGDRGVIKG 480

Query: 481 ADRFLFVIGRCSDVITL-TNNQEIHPHYIESIAYNNFSAYLRGGCLAAIKVSDTIAVVAE 540
           +DRFLFVIGRCSDVI L  NNQ+IHPHYIES +YNNFSAYLRGGC+AA+KVS TIA+VAE
Sbjct: 481 SDRFLFVIGRCSDVIKLNNNNQQIHPHYIESTSYNNFSAYLRGGCIAAVKVSGTIALVAE 540

Query: 541 MQREDKNDAETLRQICEGIRKAALIEEGIELGLVVLVKRGNVPKTTSGKVKRWLAKEKLA 600
           MQR+D++DAE LR+ICE IRKA LIEEGIELGLVVLVKRGNV KTTSGKVKRW+ KEKLA
Sbjct: 541 MQRDDRHDAELLRKICEDIRKAVLIEEGIELGLVVLVKRGNVSKTTSGKVKRWVVKEKLA 600

Query: 601 GGGMSVLMAVKFGTICGVTKDLD--GNRPLLLSLL 633
           GGG+ VLMA +FG  C   K       RP+L+S+L
Sbjct: 601 GGGLGVLMAFEFGKNCVDLKRKGEFETRPVLISML 623

BLAST of CmaCh05G002470.1 vs. TrEMBL
Match: A0A0D2S4U7_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_009G201400 PE=4 SV=1)

HSP 1 Score: 771.2 bits (1990), Expect = 1.0e-219
Identity = 386/624 (61.86%), Postives = 471/624 (75.48%), Query Frame = 1

Query: 1   MSYENFDPIFPDQPVVDLYLPVWASLPAFRSKPAFIWSEDGTADVLNEGSFLTYGQLHDS 60
           M+YEN+DPIFPDQPVVD YLP+WASL AFRSKPAFIWS+DGT +V N GS LTY QL+DS
Sbjct: 1   MNYENYDPIFPDQPVVDQYLPIWASLSAFRSKPAFIWSDDGTTNVSN-GSTLTYAQLNDS 60

Query: 61  VQFISEQLIRQVRRRDTVIVLCSPGLDLVQLIYGCQRAGLVSVPISPPDPCFENENCHHF 120
           VQ IS QL+  ++R DTVI+LC+PGL+LV++I+GCQRAGL+SVPI PPDP F  +N HH 
Sbjct: 61  VQSISIQLLHSLQRGDTVIILCTPGLELVEIIFGCQRAGLLSVPIFPPDPSFSKQNHHHL 120

Query: 121 ARALSQTKPRAVIAHEPYIVSVFRYLSSSQIDGKLALLLQSVQWISMETLRTCTAAEADQ 180
            RALSQTKP+A IAH  YI  V +YLS    D KLA +LQ+++W+  E ++      AD 
Sbjct: 121 LRALSQTKPKAAIAHHDYITKVQQYLSLRSKDKKLAQMLQNLKWVPTEDVKH---KNADS 180

Query: 181 VVSVISSKDRPFFYHSNSSYYGCKPEEPYLIQYTSGATGIPKPVVVTAGAAAHNVRAARK 240
            +              + SY GCKP+E YLIQYTSGATGIPKPV+VTAG+AAHNVR ARK
Sbjct: 181 RLD-------------SMSYDGCKPDETYLIQYTSGATGIPKPVLVTAGSAAHNVRTARK 240

Query: 241 AYDLNPNDVIVSWLPQYHDCGLMFLLLTVVSGATCVLTSPISFVTRPITWLHLITTFKAT 300
           AYDL+PN+VIVSWLPQYHDCGLMFLLLT+VSGATCVLTSP +FV RP  W+ LIT FKAT
Sbjct: 241 AYDLHPNNVIVSWLPQYHDCGLMFLLLTIVSGATCVLTSPAAFVNRPRLWIELITQFKAT 300

Query: 301 CTPVPSFTLPLVLKRVKEETPPGGDLDLCCLRNLILINEPVYRSGVEEFVDVFKAVGLNP 360
           CTPVPSFTLPLV+KR   ET     ++L  LRNLI+INEP+Y++ VEEF+ VFK  GLNP
Sbjct: 301 CTPVPSFTLPLVVKRGGVET-GSSPINLWSLRNLIIINEPIYKASVEEFLHVFKPFGLNP 360

Query: 361 GCVSPSYGLAENSTFVSTAWSGGEGGLRRCFPAMPSYRKLLPCARL-TDGSSAEIEVVVV 420
             +SPSYGLAEN TFVSTAW   +     CFP  PS+ KLLP ARL ++    +++++VV
Sbjct: 361 SSISPSYGLAENCTFVSTAWRNDDNSGNSCFPVFPSHNKLLPIARLASEEEEEDMDIIVV 420

Query: 421 NGETGEMVDDGVEGEIWISSPSNASGYLGHPLLTRDTFHCKLSNKSSHKFVRTGDRGVIK 480
           N  T E VDDG+EGEIW+SSPSNASGYLGHP LT+D F  +LSNK S  FVRTGD+G++K
Sbjct: 421 NEHTHEPVDDGIEGEIWVSSPSNASGYLGHPFLTQDIFKGRLSNKVSRCFVRTGDKGIVK 480

Query: 481 GADRFLFVIGRCSDVITLTNNQEIHPHYIESIAYNNFSAYLRGGCLAAIKVSDTIAVVAE 540
           G +RFLFV GRC++VI L N Q+IHPHYIE+ AYN+   ++RGGCLAA  VS  I +VAE
Sbjct: 481 GEERFLFVTGRCAEVIKLPNGQDIHPHYIETAAYNSCPQFIRGGCLAAFDVSRMIVLVAE 540

Query: 541 MQREDKNDAETLRQICEGIRKAALIEEGIELGLVVLVKRGNVPKTTSGKVKRWLAKEKLA 600
           MQR +K D + LR ICE IR+     E +++G+VVLVK G+VPKTTSGK++RW AK+   
Sbjct: 541 MQRSEK-DNKILRNICERIRENVFENEKVDVGMVVLVKSGSVPKTTSGKIQRWAAKQYFL 600

Query: 601 GGGMSVLMAVKFGTICGVTKDLDG 624
           GG M+ LM +KF    G  +  +G
Sbjct: 601 GGKMNALMELKFDNNGGFLRSSNG 605

BLAST of CmaCh05G002470.1 vs. TrEMBL
Match: A0A061FCL9_THECC (AMP-dependent synthetase and ligase family protein OS=Theobroma cacao GN=TCM_034053 PE=4 SV=1)

HSP 1 Score: 751.1 bits (1938), Expect = 1.1e-213
Identity = 381/620 (61.45%), Postives = 464/620 (74.84%), Query Frame = 1

Query: 1   MSYENFDPIFPDQPVVDLYLPVWASLPAFRSKPAFIWSEDGTADVLNEGSFLTYGQLHDS 60
           M+YEN+DP FPDQPVVD YLP+WASLPAFRSKPAFIW +DG+ DV ++ S LTY QL+DS
Sbjct: 1   MNYENYDPSFPDQPVVDQYLPIWASLPAFRSKPAFIWPDDGSTDV-SKSSTLTYAQLNDS 60

Query: 61  VQFISEQLIRQVRRRDTVIVLCSPGLDLVQLIYGCQRAGLVSVPISPPDPCFENENCHHF 120
           VQ IS QL+  ++R DT+++LCSPGL+LV++I+GCQRAGL+SVPI PPDP F  EN HH 
Sbjct: 61  VQSISFQLLLSLQRGDTIVILCSPGLELVEIIFGCQRAGLLSVPIIPPDPSFAKENYHHL 120

Query: 121 ARALSQTKPRAVIAHEPYIVSVFRYLSSSQIDGKLALLLQSVQWISMETLRTCTAAEADQ 180
            R LSQTK +A IAH  YI  V +YLSS   D +LA +LQ++ WIS   ++         
Sbjct: 121 LRVLSQTKLKAAIAHHDYITRVQQYLSSPSKDERLAGMLQNLIWISTGDIKH-------- 180

Query: 181 VVSVISSKDRPFFYHSNSSYYGCKPEEPYLIQYTSGATGIPKPVVVTAGAAAHNVRAARK 240
               + S     FY+      GCKP+E YLIQYTSGATGIPKPV+VTAG+AAHNVR ARK
Sbjct: 181 --KKVDSTAGSMFYN------GCKPDELYLIQYTSGATGIPKPVLVTAGSAAHNVRTARK 240

Query: 241 AYDLNPNDVIVSWLPQYHDCGLMFLLLTVVSGATCVLTSPISFVTRPITWLHLITTFKAT 300
           AYDL+PN VIVSWLPQYHDCGLMFLLLT+VSGATCVLTSP +FV RP  W+ LIT FKAT
Sbjct: 241 AYDLHPNSVIVSWLPQYHDCGLMFLLLTIVSGATCVLTSPGAFVNRPRLWIELITEFKAT 300

Query: 301 CTPVPSFTLPLVLKR--VKEETPPGGDLDLCCLRNLILINEPVYRSGVEEFVDVFKAVGL 360
           CTPVPSFTLPLV+KR  V++ + P   ++L  LRNLI+INEP+Y++ VEEF+DVFK  GL
Sbjct: 301 CTPVPSFTLPLVVKRGGVEKGSSP---INLWSLRNLIIINEPIYKASVEEFLDVFKPFGL 360

Query: 361 NPGCVSPSYGLAENSTFVSTAWSGGEGGLRRCFPAMPSYRKLLPCARLTDGSSAE-IEVV 420
           NP  +SPSYGLAEN TFVSTAW   +      F  +PS+ KLLP ARL +    E + ++
Sbjct: 361 NPSSISPSYGLAENCTFVSTAWRNNDNSGNSSFRHLPSHNKLLPSARLANEEEEEDMNII 420

Query: 421 VVNGETGEMVDDGVEGEIWISSPSNASGYLGHPLLTRDTFHCKLSNKSSHKFVRTGDRGV 480
           VVN +T E V+D +EGEIW+SSPSNASGYLGHP LT+D F  +LSNK    FVRTGDRG+
Sbjct: 421 VVNEDTHEPVEDEIEGEIWVSSPSNASGYLGHPFLTQDIFKGRLSNKVGRCFVRTGDRGI 480

Query: 481 IKGADRFLFVIGRCSDVITLTNNQEIHPHYIESIAYNNFSAYLRGGCLAAIKVSDTIAVV 540
           +KGA+RFLFV GRC DV+ L N Q++HPHYIE+ AYN     +RGGCLAA  +S  I +V
Sbjct: 481 VKGAERFLFVTGRCLDVVKLPNGQDMHPHYIETTAYNTCPQLIRGGCLAAFDISRMIVLV 540

Query: 541 AEMQREDKNDAETLRQICEGIRKAALIEEGIELGLVVLVKRGNVPKTTSGKVKRWLAKEK 600
           AEMQR +K D + LR ICE +R+  L +E +ELG+VVLVK G+VPKTTSGK++RW AK+ 
Sbjct: 541 AEMQRSEK-DNKILRDICEKMRETVLNQERVELGMVVLVKSGSVPKTTSGKIQRWAAKDN 599

Query: 601 LAGGGMSVLMAVKFGTICGV 618
             GG M VLM +KF    GV
Sbjct: 601 FLGGKMKVLMEMKFDNYHGV 599

BLAST of CmaCh05G002470.1 vs. TrEMBL
Match: M5Y045_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa022828mg PE=4 SV=1)

HSP 1 Score: 743.8 bits (1919), Expect = 1.7e-211
Identity = 378/624 (60.58%), Postives = 469/624 (75.16%), Query Frame = 1

Query: 1   MSYENFDPIFPDQPVVDLYLPVWASLPAFRSKPAFIWSEDGTADVLNEGSFLTYGQLHDS 60
           M+YEN+DP +PDQPVVDLYL VWA+LPAFRSKPAF+W EDG A  ++  S LTY QL+DS
Sbjct: 1   MNYENYDPSYPDQPVVDLYLQVWANLPAFRSKPAFVWVEDGAA--IDAKSTLTYAQLNDS 60

Query: 61  VQFISEQLIRQVRRRDTVIVLCSPGLDLVQLIYGCQRAGLVSVPISPPDPCFENENCHHF 120
           VQ I+ QL+  ++R DTV++LCSPGL+LV+ I+GCQRAGL+SVPISPPDP F N+N HH 
Sbjct: 61  VQCIASQLLVPLQRGDTVVILCSPGLELVETIFGCQRAGLLSVPISPPDPSFTNQNFHHL 120

Query: 121 ARALSQTKPRAVIAHEPYIVSVFRYLSSSQIDGKLALLLQSVQWISMETLRTCTAAEADQ 180
            RALSQTKP+A IAH  YI S+  Y+SSS  + KL  +LQS+ WIS   ++         
Sbjct: 121 IRALSQTKPKAAIAHPTYITSINHYISSSSSNKKLVHMLQSLHWISTVDIK--------- 180

Query: 181 VVSVISSKDRPFFYHSN-----SSYYGCKPEEPYLIQYTSGATGIPKPVVVTAGAAAHNV 240
                 +KDR    HS      S Y GC+ E+ YL+QYTSGATGIPKPV+VTAG+AAHNV
Sbjct: 181 ------AKDRKL-QHSQLGPKFSPYKGCRAEDVYLVQYTSGATGIPKPVLVTAGSAAHNV 240

Query: 241 RAARKAYDLNPNDVIVSWLPQYHDCGLMFLLLTVVSGATCVLTSPISFVTRPITWLHLIT 300
           R ARKAYDL+PN VIVSWLPQYHDCGLMFLLLT+VSGAT +LTSP +FV RP  WL LIT
Sbjct: 241 RTARKAYDLHPNSVIVSWLPQYHDCGLMFLLLTIVSGATSILTSPAAFVNRPRLWLELIT 300

Query: 301 TFKATCTPVPSFTLPLVLKR--VKEETPPGGDLDLCCLRNLILINEPVYRSGVEEFVDVF 360
            F+ATCTPVPSF LPLV+KR  + + T P   + L  L+NLI+INEP+YR  VE+FV+VF
Sbjct: 301 QFRATCTPVPSFALPLVVKRGGIDKGTSP---IHLWSLQNLIIINEPIYRDAVEQFVNVF 360

Query: 361 KAVGLNPGCVSPSYGLAENSTFVSTAWSGGEGGLRRC---FPAMPSYRKLLPCARL--TD 420
           +  GLNP  +SPSYGLAEN TFVST W       R C    P MPSY KLLP ARL   D
Sbjct: 361 RTFGLNPSSISPSYGLAENCTFVSTVW-------RPCGNDVPNMPSYNKLLPSARLGQND 420

Query: 421 GSSAEIEVVVVNGETGEMVDDGVEGEIWISSPSNASGYLGHPLLTRDTFHCKLSNKSSHK 480
               +++++VVN ET E V+DG+EGEIW+SSPSNASGYLGHP +TR+ ++ +L NK S  
Sbjct: 421 DELVDMDIIVVNEETHEAVEDGIEGEIWVSSPSNASGYLGHPTMTREVYYGRLKNKVSRC 480

Query: 481 FVRTGDRGVIKGADRFLFVIGRCSDVITLTNNQEIHPHYIESIAYNNFSAYLRGGCLAAI 540
           F+RTGDRGV+KG +R+LFV+GRC+DVI   N++EIHPHYIE+ AYN+   +LR GC AA 
Sbjct: 481 FLRTGDRGVVKGDERYLFVMGRCADVI--KNHEEIHPHYIETAAYNSSPGFLRAGCSAAF 540

Query: 541 KVSDTIAVVAEMQREDKNDAETLRQICEGIRKAALIEEGIELGLVVLVKRGNVPKTTSGK 600
           ++S+ + VV EMQR +K +   LR+ICEG+RK    EE +E+G+VVLV+ G+VPKTTSGK
Sbjct: 541 EISNRVVVVVEMQRSEK-EIGVLRKICEGVRKGVAEEERVEVGIVVLVRSGSVPKTTSGK 593

Query: 601 VKRWLAKEKLAGGGMSVLMAVKFG 613
           ++RW AK+KL GG MSVLM ++FG
Sbjct: 601 IQRWAAKDKLIGGKMSVLMEMRFG 593

BLAST of CmaCh05G002470.1 vs. TrEMBL
Match: A0A067KU46_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_04438 PE=4 SV=1)

HSP 1 Score: 741.9 bits (1914), Expect = 6.5e-211
Identity = 371/619 (59.94%), Postives = 458/619 (73.99%), Query Frame = 1

Query: 1   MSYENFDPIFPDQPVVDLYLPVWASLPAFRSKPAFIWSEDGTADVLNEGSFLTYGQLHDS 60
           M+YEN+DP FPDQPVVDLYLP+WA+LPAF SKP FIWSE+G+A   N  + LTY QL+ S
Sbjct: 9   MNYENYDPSFPDQPVVDLYLPIWANLPAFSSKPVFIWSENGSACAPNASNILTYAQLNKS 68

Query: 61  VQFISEQLIRQVRRRDTVIVLCSPGLDLVQLIYGCQRAGLVSVPISPPDPCFENENCHHF 120
           VQ IS QL+  ++R DTV++LC PGL+LV++I+ CQRAGL+S+PI PPDP F N N HH 
Sbjct: 69  VQSISTQLLFSLQRADTVLILCPPGLELVEMIFACQRAGLLSIPIFPPDPSFTNNNYHHL 128

Query: 121 ARALSQTKPRAVIAHEPYIVSVFRY---LSSSQIDGKLALLLQSVQWISMETLRTCTAAE 180
            R LSQTKP+A IAH  YI SV +Y   LSS+  + KLA LLQ ++WIS   LR   AA 
Sbjct: 129 IRVLSQTKPKAAIAHHDYISSVQQYISSLSSTNKNNKLAKLLQRLRWISTSDLRNDKAAG 188

Query: 181 ADQVVSVISSKDRPFFYHSNSSYYGCKPEEPYLIQYTSGATGIPKPVVVTAGAAAHNVRA 240
           +    S              SSY GC+P E YLIQYTSGATGIPKPV+VT G+A HNVR 
Sbjct: 189 SSTSQS--------------SSYDGCRPNEVYLIQYTSGATGIPKPVMVTTGSATHNVRV 248

Query: 241 ARKAYDLNPNDVIVSWLPQYHDCGLMFLLLTVVSGATCVLTSPISFVTRPITWLHLITTF 300
           ARKAYDL+PN VIVSWLPQYHDCGL FLLLTV+ GATCVLTSP +F+TRP  WL LI+ +
Sbjct: 249 ARKAYDLHPNSVIVSWLPQYHDCGLQFLLLTVICGATCVLTSPAAFITRPRLWLELISEY 308

Query: 301 KATCTPVPSFTLPLVLKR--VKEETPPGGDLDLCCLRNLILINEPVYRSGVEEFVDVFKA 360
           +ATCTPVPSFTLPLV+KR  +++ T P   ++L  L+NLI+INEP++++ VEEF+ VF  
Sbjct: 309 RATCTPVPSFTLPLVVKRGGIEKGTQP---INLWSLKNLIIINEPIFKASVEEFIYVFNP 368

Query: 361 VGLNPGCVSPSYGLAENSTFVSTAWSGGEGGLRRCFPAMPSYRKLLPCARLT---DGSSA 420
            GL+P C+SPSYGLAEN TFVSTAWS         F   P++ K+LPCARL+   +    
Sbjct: 369 FGLDPSCISPSYGLAENCTFVSTAWSRSNNN----FDNFPTHNKILPCARLSSLHEEDEE 428

Query: 421 EIEVVVVNGETGEMVDDGVEGEIWISSPSNASGYLGHPLLTRDTFHCKLSNKSSHKFVRT 480
           ++E+ VV+  T E V DG EGEIW+SSPSN  GYLGHP LTR+ F  +L NK S  FVRT
Sbjct: 429 DMEITVVDEATHEAVTDGTEGEIWVSSPSNCCGYLGHPSLTREIFQARLKNKVSKCFVRT 488

Query: 481 GDRGVIKGADRFLFVIGRCSDVITLTNNQEIHPHYIESIAYNNFSAYLRGGCLAAIKVSD 540
           GDRGVI+G +RFL+V GRCSDVI L+N QEIHPHYIE+ A N +  +LR GC AA +VS+
Sbjct: 489 GDRGVIRGEERFLYVTGRCSDVIKLSNGQEIHPHYIETAACNVYPEFLRRGCAAAFQVSN 548

Query: 541 TIAVVAEMQREDKNDAETLRQICEGIRKAALIEEGIELGLVVLVKRGNVPKTTSGKVKRW 600
           T+A++AEMQR + +D+  LRQICEGI+K  L EE +E+GLVVLVK G++PKTTSGK++RW
Sbjct: 549 TVAIIAEMQRRENDDSGVLRQICEGIQKIVLEEERVEIGLVVLVKSGSIPKTTSGKIQRW 606

Query: 601 LAKEKLAGGGMSVLMAVKF 612
            AK KL  G MS++    F
Sbjct: 609 AAKNKLTEGKMSIVTEFSF 606

BLAST of CmaCh05G002470.1 vs. TAIR10
Match: AT3G21230.1 (AT3G21230.1 4-coumarate:CoA ligase 5)

HSP 1 Score: 72.8 bits (177), Expect = 8.6e-13
Identity = 95/400 (23.75%), Postives = 163/400 (40.75%), Query Frame = 1

Query: 205 PEEPYLIQYTSGATGIPKPVVVT----AGAAAHNVRAARKAYDLNPNDVIVSWLPQYHDC 264
           PE+   + Y+SG TG+PK V++T      + A  V       +   NDVI+ +LP +H  
Sbjct: 209 PEDTVAMPYSSGTTGLPKGVMITHKGLVTSIAQKVDGENPNLNFTANDVILCFLPMFHIY 268

Query: 265 GLMFLLLTVVSGATCVLTSPISFVTRPITWLHLITTFKATCTPVPSFTLPLVLKRVKEET 324
            L  L+L+ +     +L  P  F    +  + LI  +K T  PV     P+VL  +K  +
Sbjct: 269 ALDALMLSAMRTGAALLIVP-RFELNLV--MELIQRYKVTVVPVAP---PVVLAFIK--S 328

Query: 325 PPGGDLDLCCLRNLILINEPVYRSGVEEFVDVFKAVGLNPGCVSPSYGLAENSTFVSTAW 384
           P     DL  +R ++L      +  +E+ V     +          YG+ E+ T   +  
Sbjct: 329 PETERYDLSSVR-IMLSGAATLKKELEDAV----RLKFPNAIFGQGYGMTESGTVAKSL- 388

Query: 385 SGGEGGLRRCFPAMPSYRKLLPCARLTDGSSAEIEVVVVNGETGEMVDDGVEGEIWISSP 444
                     F   P   K   C  +   +    E+ VV+ ETG  +     GEI +   
Sbjct: 389 ---------AFAKNPFKTKSGACGTVIRNA----EMKVVDTETGISLPRNKSGEICVRGH 448

Query: 445 SNASGYLGHPLLTRDTFHCKLSNKSSHKFVRTGDRGVIKGADRFLFVIGRCSDVITLTNN 504
               GYL  P  T  T            ++ TGD G +   D  +F++ R  ++I     
Sbjct: 449 QLMKGYLNDPEATARTI-------DKDGWLHTGDIGFVDDDDE-IFIVDRLKELIKFKGY 508

Query: 505 QEIHPHYIESIAYNNFSAYLRGGCLAAIK--VSDTIAVVAEMQREDKNDAETLRQICEGI 564
           Q + P  +E++  ++ S  +    + A+K  V+D + V    + +     E    +   +
Sbjct: 509 Q-VAPAELEALLISHPS--IDDAAVVAMKDEVADEVPVAFVARSQGSQLTED--DVKSYV 564

Query: 565 RKAALIEEGIELGLVVLVKRGNVPKTTSGKVKRWLAKEKL 599
            K  +  + I++   + V    +PK  SGK+ R   + KL
Sbjct: 569 NKQVVHYKRIKMVFFIEV----IPKAVSGKILRKDLRAKL 564

BLAST of CmaCh05G002470.1 vs. TAIR10
Match: AT1G65060.1 (AT1G65060.1 4-coumarate:CoA ligase 3)

HSP 1 Score: 69.7 bits (169), Expect = 7.3e-12
Identity = 129/561 (22.99%), Postives = 217/561 (38.68%), Query Frame = 1

Query: 49  GSFLTYGQLHDSVQFISEQLIRQ-VRRRDTVIVLCSPGLDLVQLIYGCQRAGLVSVPISP 108
           G   TYG+ H   + ++  L +  +R+ D +++L     + V    G    G VS   + 
Sbjct: 73  GKSYTYGETHLICRRVASGLYKLGIRKGDVIMILLQNSAEFVFSFMGASMIGAVS---TT 132

Query: 109 PDPCFENENCHHFARALSQTKPRAVIAHEPYIVSVFRYLSSSQIDGKLALLLQSVQWISM 168
            +P + ++  +   + L  +  + +I H  Y+              KL  L +++  I+ 
Sbjct: 133 ANPFYTSQELY---KQLKSSGAKLIITHSQYV-------------DKLKNLGENLTLITT 192

Query: 169 E--TLRTCTAAEADQVVSVISSKDRPFFYHSNSSYYGCKPEEPYLIQYTSGATGIPKPVV 228
           +  T   C         S + + D    +       G   ++   + ++SG TG+PK VV
Sbjct: 193 DEPTPENCLP------FSTLITDDETNPFQETVDIGG---DDAAALPFSSGTTGLPKGVV 252

Query: 229 VT----AGAAAHNVRAARKAYDLNPNDVIVSWLPQYHDCGLMFLLL-TVVSGATCVLTSP 288
           +T      + A  V        L  NDVI+  LP +H   L  +LL ++ SGAT +L   
Sbjct: 253 LTHKSLITSVAQQVDGDNPNLYLKSNDVILCVLPLFHIYSLNSVLLNSLRSGATVLLMHK 312

Query: 289 ISFVTRPITWLHLITTFKATCTPVPSFTLPLVLKRVKEETPPGGDLDLCCLRNLILINEP 348
                     L LI   + T   + +   PLV+   K   P     DL  +R ++    P
Sbjct: 313 FEIG----ALLDLIQRHRVT---IAALVPPLVIALAK--NPTVNSYDLSSVRFVLSGAAP 372

Query: 349 VYRSGVEEFVDVFKAVGLNPGCVSPSYGLAENSTFVSTAWSGGEGGLRRCFPAMPSYRKL 408
           + +    E  D  +   L    +   YG+ E    +S +           F   P   K 
Sbjct: 373 LGK----ELQDSLRR-RLPQAILGQGYGMTEAGPVLSMSLG---------FAKEPIPTKS 432

Query: 409 LPCARLTDGSSAEIEVVVVNGETGEMVDDGVEGEIWISSPSNASGYLGHPLLTRDTFHCK 468
             C  +   +    E+ VV+ ET   +     GEI I        YL  P  T  T    
Sbjct: 433 GSCGTVVRNA----ELKVVHLETRLSLGYNQPGEICIRGQQIMKEYLNDPEATSATI--- 492

Query: 469 LSNKSSHKFVRTGDRGVIKGADRFLFVIGRCSDVITLTNNQEIHPHYIESIAYNNFSAYL 528
                   ++ TGD G +   D  +F++ R  +VI     Q + P  +ES+  N+ S   
Sbjct: 493 ----DEEGWLHTGDIGYVDEDDE-IFIVDRLKEVIKFKGFQ-VPPAELESLLINHHSI-- 552

Query: 529 RGGCLAAIKVSDTIA---VVAEMQREDKNDAETLRQICEGIRKAALIEEGIELGLVVLVK 588
                A +  +D +A    VA + R + ND  T   + E + K  +  + +     V   
Sbjct: 553 --ADAAVVPQNDEVAGEVPVAFVVRSNGNDI-TEEDVKEYVAKQVVFYKRLHKVFFV--- 560

Query: 589 RGNVPKTTSGKVKRWLAKEKL 599
             ++PK+ SGK+ R   K KL
Sbjct: 613 -ASIPKSPSGKILRKDLKAKL 560

BLAST of CmaCh05G002470.1 vs. TAIR10
Match: AT3G21240.1 (AT3G21240.1 4-coumarate:CoA ligase 2)

HSP 1 Score: 69.3 bits (168), Expect = 9.5e-12
Identity = 133/607 (21.91%), Postives = 234/607 (38.55%), Query Frame = 1

Query: 17  DLYLP--------VWASLPAFRSKPAFIWSEDGTADVLNEGSFLTYGQLHDSVQFISEQL 76
           D+Y+P        ++ ++  F +KP  I   +G       G   TY  +H + + ++  L
Sbjct: 29  DIYIPNHLPLHDYIFENISEFAAKPCLI---NGPT-----GEVYTYADVHVTSRKLAAGL 88

Query: 77  IRQ-VRRRDTVIVLCSPGLDLVQLIYGCQRAGLVSVPISPPDPCFENENCHHFARALSQT 136
               V++ D V++L     ++V         G ++   +P            F  A    
Sbjct: 89  HNLGVKQHDVVMILLPNSPEVVLTFLAASFIGAITTSANP-----------FFTPAEISK 148

Query: 137 KPRAVIAHEPYIVSVFRY---LSSSQIDGKLALLLQSVQWISMETLRTCTAAEADQV-VS 196
           + +A  A    IV+  RY   + + Q DG L +   S   I    LR     ++++  V 
Sbjct: 149 QAKASAAK--LIVTQSRYVDKIKNLQNDGVLIVTTDS-DAIPENCLRFSELTQSEEPRVD 208

Query: 197 VISSKDRPFFYHSNSSYYGCKPEEPYLIQYTSGATGIPKPVVVT----AGAAAHNVRAAR 256
            I  K                PE+   + ++SG TG+PK V++T      + A  V    
Sbjct: 209 SIPEK--------------ISPEDVVALPFSSGTTGLPKGVMLTHKGLVTSVAQQVDGEN 268

Query: 257 KAYDLNPNDVIVSWLPQYHDCGLMFLLLTVVSGATCVLTSPISFVTRPITWLHLITTFKA 316
                N +DVI+  LP +H   L  ++L  +     +L  P   +T  +  +       A
Sbjct: 269 PNLYFNRDDVILCVLPMFHIYALNSIMLCSLRVGATILIMPKFEITLLLEQIQRCKVTVA 328

Query: 317 TCTPVPSFTLPLVLKRVKEETPPGGDLDLCCLRNLILINEPVYRSGVEEFVDVFKAVGLN 376
              P      P+VL   K  +P     DL  +R +     P+ +   +     F    L 
Sbjct: 329 MVVP------PIVLAIAK--SPETEKYDLSSVRMVKSGAAPLGKELEDAISAKFPNAKLG 388

Query: 377 PGCVSPSYGLAENSTFVSTAWSGGEGGLRRCFPAMPSYRKLLPCARLTDGSSAEIEVVVV 436
            G     YG+ E    ++ +     G  +  FP      K   C  +   +    E+ ++
Sbjct: 389 QG-----YGMTEAGPVLAMSL----GFAKEPFPV-----KSGACGTVVRNA----EMKIL 448

Query: 437 NGETGEMVDDGVEGEIWISSPSNASGYLGHPLLTRDTFHCKLSNKSSHKFVRTGDRGVIK 496
           + +TG+ +     GEI I       GYL  PL T  T            ++ TGD G I 
Sbjct: 449 DPDTGDSLPRNKPGEICIRGNQIMKGYLNDPLATASTI-------DKDGWLHTGDVGFID 508

Query: 497 GADRFLFVIGRCSDVITLTNNQEIHPHYIESIAYNNFSAYLRGGCLAAIKVSDT--IAVV 556
             D  LF++ R  ++I     Q + P  +ES+   +    +    + A+K  D   + V 
Sbjct: 509 DDDE-LFIVDRLKELIKYKGFQ-VAPAELESLLIGH--PEINDVAVVAMKEEDAGEVPVA 556

Query: 557 AEMQREDKNDAETLRQICEGIRKAALIEEGIELGLVVLVKRGNVPKTTSGKVKRWLAKEK 605
             ++ +D N +E   +I + + K  +  + I      +    ++PK  SGK+ R   + +
Sbjct: 569 FVVRSKDSNISED--EIKQFVSKQVVFYKRIN----KVFFTDSIPKAPSGKILRKDLRAR 556

BLAST of CmaCh05G002470.1 vs. TAIR10
Match: AT1G51680.1 (AT1G51680.1 4-coumarate:CoA ligase 1)

HSP 1 Score: 65.1 bits (157), Expect = 1.8e-10
Identity = 140/632 (22.15%), Postives = 239/632 (37.82%), Query Frame = 1

Query: 5   NFDPIFPDQPVVDLYLP--------VWASLPAFRSKPAFIWSEDGTADVLNEGSFLTYGQ 64
           N D IF  + + D+Y+P        ++ ++  F +KP  I   +G       G   TY  
Sbjct: 20  NSDVIFRSK-LPDIYIPNHLSLHDYIFQNISEFATKPCLI---NGPT-----GHVYTYSD 79

Query: 65  LHDSVQFISEQLIRQVRRRDTVIVLCSPGLDLVQLIYGCQRAGLVSVPISPPDPCFENEN 124
           +H   + I+    +    ++ V++L  P     + +     A       +  +P F    
Sbjct: 80  VHVISRQIAANFHKLGVNQNDVVMLLLPNCP--EFVLSFLAASFRGATATAANPFFTPAE 139

Query: 125 CHHFARALSQTKPRAVIAHEPYIVSVFRYLSSSQIDGKLALLLQSVQWISME------TL 184
               A+A S TK   +I  E   V   + L +   DG + + +   + + +       T 
Sbjct: 140 IAKQAKA-SNTK---LIITEARYVDKIKPLQND--DGVVIVCIDDNESVPIPEGCLRFTE 199

Query: 185 RTCTAAEADQVVSVISSKDRPFFYHSNSSYYGCKPEEPYLIQYTSGATGIPKPVVVTAGA 244
            T +  EA +V+  +                   P++   + Y+SG TG+PK V++T   
Sbjct: 200 LTQSTTEASEVIDSVE----------------ISPDDVVALPYSSGTTGLPKGVMLTHKG 259

Query: 245 AAHNVRAARKAYDLNPN------DVIVSWLPQYHDCGLMFLLLTVVSGATCVLTSPISFV 304
              +V  A++    NPN      DVI+  LP +H   L  ++L  +     +L  P   +
Sbjct: 260 LVTSV--AQQVDGENPNLYFHSDDVILCVLPMFHIYALNSIMLCGLRVGAAILIMPKFEI 319

Query: 305 TRPITWLHLITTFKATCTP-VPSFTLPLVLKRVKEETPPGGDLDLCCLRNLILINEPVYR 364
                 L LI   K T  P VP   L +      E+       DL  +R        V +
Sbjct: 320 N---LLLELIQRCKVTVAPMVPPIVLAIAKSSETEK------YDLSSIR--------VVK 379

Query: 365 SGV----EEFVDVFKAVGLNPGCVSPSYGLAENSTFVSTAWSGGEGGLRRCFPAMPSYRK 424
           SG     +E  D   A   N   +   YG+ E    ++ +     G  +  FP      K
Sbjct: 380 SGAAPLGKELEDAVNAKFPN-AKLGQGYGMTEAGPVLAMSL----GFAKEPFPV-----K 439

Query: 425 LLPCARLTDGSSAEIEVVVVNGETGEMVDDGVEGEIWISSPSNASGYLGHPLLTRDTFHC 484
              C  +   +    E+ +V+ +TG+ +     GEI I       GYL +P  T +T   
Sbjct: 440 SGACGTVVRNA----EMKIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAETI-- 499

Query: 485 KLSNKSSHKFVRTGDRGVIKGADRFLFVIGRCSDVITLTNNQEIHPHYIESIAYNNFSAY 544
                    ++ TGD G+I   D  LF++ R  ++I     Q + P  +E++   +    
Sbjct: 500 -----DKDGWLHTGDIGLIDDDDE-LFIVDRLKELIKYKGFQ-VAPAELEALLIGH---- 559

Query: 545 LRGGCLAAIKVSDTIAVVAEMQREDKNDAETLRQICEGIRKAALIEEGIELGL---VVLV 602
                         I  VA +  +++   E         + + L E+ ++  +   VV  
Sbjct: 560 ------------PDITDVAVVAMKEEAAGEVPVAFVVKSKDSELSEDDVKQFVSKQVVFY 560

BLAST of CmaCh05G002470.1 vs. TAIR10
Match: AT1G20490.1 (AT1G20490.1 AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 60.8 bits (146), Expect = 3.4e-09
Identity = 85/417 (20.38%), Postives = 165/417 (39.57%), Query Frame = 1

Query: 49  GSFLTYGQLHDSVQFISEQLIRQV--RRRDTVIVLCSPGLDLVQLIYGCQRAGLVSVPIS 108
           G  LT+  L   V  +++ L  +V  RR D V++L    + +  +       G V    +
Sbjct: 61  GHRLTFSDLWRVVDRVADCLYHEVGIRRGDVVLILSPNSIYIPVVCLSVMSLGAVVTTAN 120

Query: 109 PPDPCFENENCHHFARALSQTKPRAVIAHEPYIVSVFRYLSSSQIDGKLALLLQSVQWIS 168
             +   E       ++ ++Q+ P  V              ++SQ+  KLA  + SV    
Sbjct: 121 TLNTSGE------ISKQIAQSNPTLVF-------------TTSQLAPKLAAAI-SVVLTD 180

Query: 169 METLRTCTAAEADQVVSVISSKDRPFFYHSNSSYYGCKPEEPYLIQYTSGATGIPKPVVV 228
            E  +        +VV ++S   +               ++  ++ Y+SG TG  K V+ 
Sbjct: 181 EEDEKRVELTSGVRVVGILSEMMKKET-SGQRVRDRVNQDDTAMMLYSSGTTGTSKGVI- 240

Query: 229 TAGAAAHNVRAARKAY---DLNPNDVIVSWLPQYHDCGLM-FLLLTVVSGATCVLTSPIS 288
              ++  N+ A    Y       +++ V  +P +H  GL+ F + +V SG+T V+     
Sbjct: 241 ---SSHRNLTAYVAKYIDDKWKRDEIFVCTVPMFHSFGLLAFAMGSVASGSTVVILRRFG 300

Query: 289 FVTRPITWLHLITTFKATCTPVPSFTLPLVLKRVKEETPPGGDLDLCCLRNLILINEPVY 348
                   +  +  +KAT   + S   P+++  +          DL  LR +     P+ 
Sbjct: 301 LDDM----MQAVEKYKAT---ILSLAPPVLVAMINGADQLKAKYDLTSLRKVRCGGAPLS 360

Query: 349 RSGVEEFVDVFKAVGLNPG-CVSPSYGLAENSTFVSTAWSGGEGGLRRCFPAMPSYRKLL 408
           +  ++ F++ +  V +  G  ++ S+G   ++  V  +   G  GL              
Sbjct: 361 KEVMDSFLEKYPTVNIFQGYALTESHGSGASTESVEESLKYGAVGLL------------- 420

Query: 409 PCARLTDGSSAEIEVVVVNGETGEMVDDGVEGEIWISSPSNASGYLGHPLLTRDTFH 459
                    S+ IE  +V+ +TG ++     GE+W+  PS + GY G+   T +T +
Sbjct: 421 ---------SSGIEARIVDPDTGRVMGVNQPGELWLKGPSISKGYFGNEEATNETIN 423

BLAST of CmaCh05G002470.1 vs. NCBI nr
Match: gi|659087949|ref|XP_008444721.1| (PREDICTED: LOW QUALITY PROTEIN: 4-coumarate--CoA ligase-like 9 [Cucumis melo])

HSP 1 Score: 963.0 bits (2488), Expect = 2.6e-277
Identity = 488/640 (76.25%), Postives = 540/640 (84.38%), Query Frame = 1

Query: 1   MSYENFDPIFPDQPVVDLYLPVWASLPAFRSKPAFIWSEDGTADVLNEGSFLTYGQLHDS 60
           M YENFDP+FP+QPVVD YLPVWASLPAF SKPAFIW+EDGT + +NEGSFLTY QLHD+
Sbjct: 1   MLYENFDPLFPEQPVVDRYLPVWASLPAFGSKPAFIWTEDGTVEAINEGSFLTYRQLHDT 60

Query: 61  VQFISEQLIRQVRRRDTVIVLCSPGLDLVQLIYGCQRAGLVSVPISPPDPCFENENCHHF 120
           VQ I+EQL+RQ+RRRDTV+VLCS GLD VQLIYGCQRAGLVSVPISPPD  FENENCHH 
Sbjct: 61  VQLITEQLLRQLRRRDTVVVLCSAGLDFVQLIYGCQRAGLVSVPISPPDVFFENENCHHL 120

Query: 121 ARALSQTKPRAVIAHEPYIVSVFRYLSSSQIDGKLALLLQSVQWISMETLRTCTAAEADQ 180
            RALSQTKPRA IAH+ YI +VFRY+SSS  D KLALLL+SVQWISME+L+     E  +
Sbjct: 121 GRALSQTKPRAAIAHQSYITTVFRYISSSPADEKLALLLKSVQWISMESLK-----EPHK 180

Query: 181 VVSVISSKDRPFFYHSNSSYYGCKPEEPYLIQYTSGATGIPKPVVVTAGAAAHNVRAARK 240
              +   K   F +HS SSY GC P++PYLIQYTSGAT I KPVV+TAGAAAHNVRAARK
Sbjct: 181 ESELNQHKHHSFSHHS-SSYSGCNPDDPYLIQYTSGATAISKPVVITAGAAAHNVRAARK 240

Query: 241 AYDLNPNDVIVSWLPQYHDCGLMFLLLTVVSGATCVLTSPISFVTRPITWLHLITTFKAT 300
           AYDLNPNDVIVSWLPQ+HDCGLMFLLLTV++GATCVLTSPISFVT+PITWLHLIT FKAT
Sbjct: 241 AYDLNPNDVIVSWLPQFHDCGLMFLLLTVITGATCVLTSPISFVTQPITWLHLITAFKAT 300

Query: 301 CTPVPSFTLPLVLKRV-KEETPPGGDLDLCCLRNLILINEPVYRSGVEEFVDVFKAVGLN 360
           CTPVPSFTLPLVLKRV    +   G+LDL  LRNLILINEP+YRS VEEFVDVFKAVGL+
Sbjct: 301 CTPVPSFTLPLVLKRVGNYSSGRAGNLDLRSLRNLILINEPIYRSTVEEFVDVFKAVGLD 360

Query: 361 PGCVSPSYGLAENSTFVSTAWSGGEGGLRRCFPAMPSYRKLLPCARLTDGSSAEIEVVVV 420
           PGCVSPSYGLAEN TFVSTAW GG G     FPAMPSYRKLLP  RL DG   EIEVVVV
Sbjct: 361 PGCVSPSYGLAENCTFVSTAWCGGGG-----FPAMPSYRKLLPSGRLRDGMCKEIEVVVV 420

Query: 421 NGETGEMVDDGVEGEIWISSPSNASGYLGHPLLTRDTFHCKLSNKSSHKFVRTGDRGVIK 480
           N ETGE+V+DGVEGEIWISSPSNA GYLGHP LT +TFH KL NK+S  FVRTGDRGVIK
Sbjct: 421 NEETGEVVEDGVEGEIWISSPSNALGYLGHPSLTEETFHRKLKNKASLNFVRTGDRGVIK 480

Query: 481 GADRFLFVIGRCSDVITLT-NNQEIHPHYIESIAYNNFSAYLRGGCLAAIKVSD-TIAVV 540
           G+DRFLFVIGRCSDVI    NNQ+IHPHYIES +Y NFSAYLRGGC+AA+K+S  T+A+V
Sbjct: 481 GSDRFLFVIGRCSDVIKFNDNNQQIHPHYIESTSYKNFSAYLRGGCIAAVKISSGTVALV 540

Query: 541 AEMQREDKNDAETLRQICEGIRKAALIEEGIELGLVVLVKRGNVPKTTSGKVKRWLAKEK 600
           AEMQR+D+NDAE LR+ICE IRKA LIEEGIELG+VVLVKRGNV KTTSGKVKRW+ KEK
Sbjct: 541 AEMQRDDRNDAELLRKICEEIRKAVLIEEGIELGVVVLVKRGNVSKTTSGKVKRWVVKEK 600

Query: 601 LAGGGMSVLMAVKFGTICGVTKDLD-----GNRPLLLSLL 633
           L GGG+ VLMAV+FG  C   KDL+     G RP+LLS+L
Sbjct: 601 LGGGGLEVLMAVEFGKDCEGLKDLERKGEFGTRPVLLSML 629

BLAST of CmaCh05G002470.1 vs. NCBI nr
Match: gi|778671360|ref|XP_011649625.1| (PREDICTED: disco-interacting protein 2 homolog C-like [Cucumis sativus])

HSP 1 Score: 951.0 bits (2457), Expect = 1.0e-273
Identity = 483/635 (76.06%), Postives = 536/635 (84.41%), Query Frame = 1

Query: 1   MSYENFDPIFPDQPVVDLYLPVWASLPAFRSKPAFIWSEDGTADVLNEGSFLTYGQLHDS 60
           M YENFDP+FP+QPVVD YLPVWASLPAF+SKPAFIWSEDGTA  +NE SFLTY QLHDS
Sbjct: 1   MLYENFDPLFPEQPVVDRYLPVWASLPAFQSKPAFIWSEDGTAKAINEDSFLTYRQLHDS 60

Query: 61  VQFISEQLIRQVRRRDTVIVLCSPGLDLVQLIYGCQRAGLVSVPISPPDPCFENENCHHF 120
           VQ I+EQL+RQ+RRRDTV+VLCS GLD VQLIYGCQRAGLVSVPISPPD   ENENCHH 
Sbjct: 61  VQLITEQLLRQLRRRDTVVVLCSAGLDFVQLIYGCQRAGLVSVPISPPDVFSENENCHHL 120

Query: 121 ARALSQTKPRAVIAHEPYIVSVFRYLSSSQIDGKLALLLQSVQWISMETLRTCTAAEADQ 180
           ARALSQTKPRA IAH+ YI +VFRY+SSS  D KLALLL+ V+WISME+L+     +  +
Sbjct: 121 ARALSQTKPRAAIAHQSYINTVFRYISSSA-DKKLALLLKGVRWISMESLK-----QPHK 180

Query: 181 VVSVISSKDRPFFYHSNSSYYGCKPEEPYLIQYTSGATGIPKPVVVTAGAAAHNVRAARK 240
              +   K +  FYHS SSY GC P++PYLIQYTSGAT I K VV+TAGAAAHNVRAARK
Sbjct: 181 ESELNQHKHQSSFYHS-SSYSGCNPDDPYLIQYTSGATAISKAVVITAGAAAHNVRAARK 240

Query: 241 AYDLNPNDVIVSWLPQYHDCGLMFLLLTVVSGATCVLTSPISFVTRPITWLHLITTFKAT 300
           AYDLNPND+IVSWLPQYHDCGLMFLLLTV++GATCVLTSPISFVT+PITWLHLIT FKAT
Sbjct: 241 AYDLNPNDLIVSWLPQYHDCGLMFLLLTVITGATCVLTSPISFVTQPITWLHLITAFKAT 300

Query: 301 CTPVPSFTLPLVLKRVKEETPPGGDLDLCCLRNLILINEPVYRSGVEEFVDVFKAVGLNP 360
           CTPVPSFTLPLVLKRV   +   G+LDL  LRNLILINEP+YRS VEEFV+VFK VGL+P
Sbjct: 301 CTPVPSFTLPLVLKRVGYSSGRAGNLDLRSLRNLILINEPIYRSTVEEFVEVFKTVGLDP 360

Query: 361 GCVSPSYGLAENSTFVSTAWSGGEGGLRRCFPAMPSYRKLLPCARLTDGSSAEIEVVVVN 420
           GCVSPSYGLAEN TFVSTAW GG G     FPAMPSYR+LLP  RL DG   EIEVVVVN
Sbjct: 361 GCVSPSYGLAENCTFVSTAWCGGGG-----FPAMPSYRQLLPSGRLRDGMCKEIEVVVVN 420

Query: 421 GETGEMVDDGVEGEIWISSPSNASGYLGHPLLTRDTFHCKLSNKSSHKFVRTGDRGVIKG 480
            ETGE+V+DGVEGEIW+SSPSNA GYLGHP +T +TFH KL NKSS  FVRTGDRGVIKG
Sbjct: 421 EETGEVVEDGVEGEIWVSSPSNALGYLGHPSVTEETFHSKLKNKSSLNFVRTGDRGVIKG 480

Query: 481 ADRFLFVIGRCSDVITL-TNNQEIHPHYIESIAYNNFSAYLRGGCLAAIKVSDTIAVVAE 540
           +DRFLFVIGRCSDVI L  NNQ+IHPHYIES +YNNFSAYLRGGC+AA+KVS TIA+VAE
Sbjct: 481 SDRFLFVIGRCSDVIKLNNNNQQIHPHYIESTSYNNFSAYLRGGCIAAVKVSGTIALVAE 540

Query: 541 MQREDKNDAETLRQICEGIRKAALIEEGIELGLVVLVKRGNVPKTTSGKVKRWLAKEKLA 600
           MQR+D++DAE LR+ICE IRKA LIEEGIELGLVVLVKRGNV KTTSGKVKRW+ KEKLA
Sbjct: 541 MQRDDRHDAELLRKICEDIRKAVLIEEGIELGLVVLVKRGNVSKTTSGKVKRWVVKEKLA 600

Query: 601 GGGMSVLMAVKFGTICGVTKDLD--GNRPLLLSLL 633
           GGG+ VLMA +FG  C   K       RP+L+S+L
Sbjct: 601 GGGLGVLMAFEFGKNCVDLKRKGEFETRPVLISML 623

BLAST of CmaCh05G002470.1 vs. NCBI nr
Match: gi|823224298|ref|XP_012444917.1| (PREDICTED: disco-interacting protein 2 homolog C isoform X1 [Gossypium raimondii])

HSP 1 Score: 771.2 bits (1990), Expect = 1.4e-219
Identity = 386/624 (61.86%), Postives = 471/624 (75.48%), Query Frame = 1

Query: 1   MSYENFDPIFPDQPVVDLYLPVWASLPAFRSKPAFIWSEDGTADVLNEGSFLTYGQLHDS 60
           M+YEN+DPIFPDQPVVD YLP+WASL AFRSKPAFIWS+DGT +V N GS LTY QL+DS
Sbjct: 1   MNYENYDPIFPDQPVVDQYLPIWASLSAFRSKPAFIWSDDGTTNVSN-GSTLTYAQLNDS 60

Query: 61  VQFISEQLIRQVRRRDTVIVLCSPGLDLVQLIYGCQRAGLVSVPISPPDPCFENENCHHF 120
           VQ IS QL+  ++R DTVI+LC+PGL+LV++I+GCQRAGL+SVPI PPDP F  +N HH 
Sbjct: 61  VQSISIQLLHSLQRGDTVIILCTPGLELVEIIFGCQRAGLLSVPIFPPDPSFSKQNHHHL 120

Query: 121 ARALSQTKPRAVIAHEPYIVSVFRYLSSSQIDGKLALLLQSVQWISMETLRTCTAAEADQ 180
            RALSQTKP+A IAH  YI  V +YLS    D KLA +LQ+++W+  E ++      AD 
Sbjct: 121 LRALSQTKPKAAIAHHDYITKVQQYLSLRSKDKKLAQMLQNLKWVPTEDVKH---KNADS 180

Query: 181 VVSVISSKDRPFFYHSNSSYYGCKPEEPYLIQYTSGATGIPKPVVVTAGAAAHNVRAARK 240
            +              + SY GCKP+E YLIQYTSGATGIPKPV+VTAG+AAHNVR ARK
Sbjct: 181 RLD-------------SMSYDGCKPDETYLIQYTSGATGIPKPVLVTAGSAAHNVRTARK 240

Query: 241 AYDLNPNDVIVSWLPQYHDCGLMFLLLTVVSGATCVLTSPISFVTRPITWLHLITTFKAT 300
           AYDL+PN+VIVSWLPQYHDCGLMFLLLT+VSGATCVLTSP +FV RP  W+ LIT FKAT
Sbjct: 241 AYDLHPNNVIVSWLPQYHDCGLMFLLLTIVSGATCVLTSPAAFVNRPRLWIELITQFKAT 300

Query: 301 CTPVPSFTLPLVLKRVKEETPPGGDLDLCCLRNLILINEPVYRSGVEEFVDVFKAVGLNP 360
           CTPVPSFTLPLV+KR   ET     ++L  LRNLI+INEP+Y++ VEEF+ VFK  GLNP
Sbjct: 301 CTPVPSFTLPLVVKRGGVET-GSSPINLWSLRNLIIINEPIYKASVEEFLHVFKPFGLNP 360

Query: 361 GCVSPSYGLAENSTFVSTAWSGGEGGLRRCFPAMPSYRKLLPCARL-TDGSSAEIEVVVV 420
             +SPSYGLAEN TFVSTAW   +     CFP  PS+ KLLP ARL ++    +++++VV
Sbjct: 361 SSISPSYGLAENCTFVSTAWRNDDNSGNSCFPVFPSHNKLLPIARLASEEEEEDMDIIVV 420

Query: 421 NGETGEMVDDGVEGEIWISSPSNASGYLGHPLLTRDTFHCKLSNKSSHKFVRTGDRGVIK 480
           N  T E VDDG+EGEIW+SSPSNASGYLGHP LT+D F  +LSNK S  FVRTGD+G++K
Sbjct: 421 NEHTHEPVDDGIEGEIWVSSPSNASGYLGHPFLTQDIFKGRLSNKVSRCFVRTGDKGIVK 480

Query: 481 GADRFLFVIGRCSDVITLTNNQEIHPHYIESIAYNNFSAYLRGGCLAAIKVSDTIAVVAE 540
           G +RFLFV GRC++VI L N Q+IHPHYIE+ AYN+   ++RGGCLAA  VS  I +VAE
Sbjct: 481 GEERFLFVTGRCAEVIKLPNGQDIHPHYIETAAYNSCPQFIRGGCLAAFDVSRMIVLVAE 540

Query: 541 MQREDKNDAETLRQICEGIRKAALIEEGIELGLVVLVKRGNVPKTTSGKVKRWLAKEKLA 600
           MQR +K D + LR ICE IR+     E +++G+VVLVK G+VPKTTSGK++RW AK+   
Sbjct: 541 MQRSEK-DNKILRNICERIRENVFENEKVDVGMVVLVKSGSVPKTTSGKIQRWAAKQYFL 600

Query: 601 GGGMSVLMAVKFGTICGVTKDLDG 624
           GG M+ LM +KF    G  +  +G
Sbjct: 601 GGKMNALMELKFDNNGGFLRSSNG 605

BLAST of CmaCh05G002470.1 vs. NCBI nr
Match: gi|694317137|ref|XP_009336914.1| (PREDICTED: disco-interacting protein 2 homolog B-A-like [Pyrus x bretschneideri])

HSP 1 Score: 751.9 bits (1940), Expect = 9.0e-214
Identity = 380/628 (60.51%), Postives = 469/628 (74.68%), Query Frame = 1

Query: 1   MSYENFDPIFPDQPVVDLYLPVWASLPAFRSKPAFIWSEDGTADVLNEGSFLTYGQLHDS 60
           M YEN+DP +PDQPVVDLYLPVWASLPAFRSKPAF+W +DG+A+     S LT+GQL+DS
Sbjct: 1   MDYENYDPSYPDQPVVDLYLPVWASLPAFRSKPAFVWVDDGSANEAT--STLTFGQLNDS 60

Query: 61  VQFISEQLIRQVRRRDTVIVLCSPGLDLVQLIYGCQRAGLVSVPISPPDPCFENENCHHF 120
           VQ I+ QL+  ++R D V++LCSPGL+LV+ I+GCQRAGL+SVP+SPPDP F N+N HH 
Sbjct: 61  VQCIASQLLTPLQRGDAVVILCSPGLELVETIFGCQRAGLLSVPVSPPDPSFTNQNFHHL 120

Query: 121 ARALSQTKPRAVIAHEPYIVSVFRYLSSSQIDGKLALLLQSVQWISMETLRTCTAAEADQ 180
            RALSQTKP+A +AH  YI  +  Y+SS   D  L   LQ+++WI+++ ++T        
Sbjct: 121 IRALSQTKPKAALAHSTYITRIQHYISSPSSDKDLVQRLQNLRWIAIDDIKT-------- 180

Query: 181 VVSVISSKDRPFFYHSNSSYYGCKPEEPYLIQYTSGATGIPKPVVVTAGAAAHNVRAARK 240
                  KD       +S Y GC+ E+ YL+QYTSGATGIPKPV+VTAG+AAHNVR ARK
Sbjct: 181 -------KDTTL---QHSPYKGCRAEDLYLVQYTSGATGIPKPVLVTAGSAAHNVRTARK 240

Query: 241 AYDLNPNDVIVSWLPQYHDCGLMFLLLTVVSGATCVLTSPISFVTRPITWLHLITTFKAT 300
           AYDL+PN VIVSWLPQYHDCGLMFLLLT+VSGATCVLTSP +FV RP  WL LI+ FKAT
Sbjct: 241 AYDLHPNSVIVSWLPQYHDCGLMFLLLTIVSGATCVLTSPGAFVNRPRLWLELISNFKAT 300

Query: 301 CTPVPSFTLPLVLKR--VKEETPPGGDLDLCCLRNLILINEPVYRSGVEEFVDVFKAVGL 360
           CTPVPSFTLPLV+KR  V + T P   ++L  ++NLI+INEP+YR  VE+FVDVFK  GL
Sbjct: 301 CTPVPSFTLPLVVKRGGVDKGTSP---INLWSMKNLIIINEPIYRDAVEQFVDVFKPYGL 360

Query: 361 NPGCVSPSYGLAENSTFVSTAWSGGEGGLRRCFPAMPSYRKLLPCARL--TDGSSAEIEV 420
           NP  +SPSYGLAEN TFVSTAW        R    +PS+ KLLP ARL   D    ++ +
Sbjct: 361 NPSSISPSYGLAENCTFVSTAW--------RSDTPLPSHNKLLPSARLNENDDQLEDMNI 420

Query: 421 VVVNGETGEMVDDGVEGEIWISSPSNASGYLGHPLLTRDTFHCKLSNKSSHKFVRTGDRG 480
           VVVN ET E V+DG+EGEIW+SSPSNASGYL HP LTR+ ++ +L NK S  F+RTGDRG
Sbjct: 421 VVVNEETHEAVEDGIEGEIWVSSPSNASGYLAHPNLTREVYYGRLKNKLSRPFLRTGDRG 480

Query: 481 VIKGADRFLFVIGRCSDVITLTNN---QEIHPHYIESIAYNNFSAYLRGGCLAAIKVSDT 540
           V+KG DR+LFV GRC+DVI L N+    EIHPHY+E+ AYN+   +LRGGCLAA +VS+T
Sbjct: 481 VVKGEDRYLFVTGRCADVIRLRNDDLGMEIHPHYVETAAYNSNPGFLRGGCLAAFEVSNT 540

Query: 541 IAVVAEMQREDKNDAETLRQICEGIRKAALIEEGIELGLVVLVKRGNVPKTTSGKVKRWL 600
           + VVAE QR +K D   LR+IC+G+R   ++EEG+E+G VVLV+ G VPKTTSGK++RW 
Sbjct: 541 VVVVAETQRMEK-DIGVLRKICDGVRHGVVMEEGVEVGTVVLVRSGCVPKTTSGKIQRWA 596

Query: 601 AKEKLAGGGMSVLMAVKFGTICGVTKDL 622
           AK+KL GG M VLM V+FG   G    L
Sbjct: 601 AKDKLVGGKMGVLMEVRFGDYGGSVPSL 596

BLAST of CmaCh05G002470.1 vs. NCBI nr
Match: gi|590593402|ref|XP_007017556.1| (AMP-dependent synthetase and ligase family protein [Theobroma cacao])

HSP 1 Score: 751.1 bits (1938), Expect = 1.5e-213
Identity = 381/620 (61.45%), Postives = 464/620 (74.84%), Query Frame = 1

Query: 1   MSYENFDPIFPDQPVVDLYLPVWASLPAFRSKPAFIWSEDGTADVLNEGSFLTYGQLHDS 60
           M+YEN+DP FPDQPVVD YLP+WASLPAFRSKPAFIW +DG+ DV ++ S LTY QL+DS
Sbjct: 1   MNYENYDPSFPDQPVVDQYLPIWASLPAFRSKPAFIWPDDGSTDV-SKSSTLTYAQLNDS 60

Query: 61  VQFISEQLIRQVRRRDTVIVLCSPGLDLVQLIYGCQRAGLVSVPISPPDPCFENENCHHF 120
           VQ IS QL+  ++R DT+++LCSPGL+LV++I+GCQRAGL+SVPI PPDP F  EN HH 
Sbjct: 61  VQSISFQLLLSLQRGDTIVILCSPGLELVEIIFGCQRAGLLSVPIIPPDPSFAKENYHHL 120

Query: 121 ARALSQTKPRAVIAHEPYIVSVFRYLSSSQIDGKLALLLQSVQWISMETLRTCTAAEADQ 180
            R LSQTK +A IAH  YI  V +YLSS   D +LA +LQ++ WIS   ++         
Sbjct: 121 LRVLSQTKLKAAIAHHDYITRVQQYLSSPSKDERLAGMLQNLIWISTGDIKH-------- 180

Query: 181 VVSVISSKDRPFFYHSNSSYYGCKPEEPYLIQYTSGATGIPKPVVVTAGAAAHNVRAARK 240
               + S     FY+      GCKP+E YLIQYTSGATGIPKPV+VTAG+AAHNVR ARK
Sbjct: 181 --KKVDSTAGSMFYN------GCKPDELYLIQYTSGATGIPKPVLVTAGSAAHNVRTARK 240

Query: 241 AYDLNPNDVIVSWLPQYHDCGLMFLLLTVVSGATCVLTSPISFVTRPITWLHLITTFKAT 300
           AYDL+PN VIVSWLPQYHDCGLMFLLLT+VSGATCVLTSP +FV RP  W+ LIT FKAT
Sbjct: 241 AYDLHPNSVIVSWLPQYHDCGLMFLLLTIVSGATCVLTSPGAFVNRPRLWIELITEFKAT 300

Query: 301 CTPVPSFTLPLVLKR--VKEETPPGGDLDLCCLRNLILINEPVYRSGVEEFVDVFKAVGL 360
           CTPVPSFTLPLV+KR  V++ + P   ++L  LRNLI+INEP+Y++ VEEF+DVFK  GL
Sbjct: 301 CTPVPSFTLPLVVKRGGVEKGSSP---INLWSLRNLIIINEPIYKASVEEFLDVFKPFGL 360

Query: 361 NPGCVSPSYGLAENSTFVSTAWSGGEGGLRRCFPAMPSYRKLLPCARLTDGSSAE-IEVV 420
           NP  +SPSYGLAEN TFVSTAW   +      F  +PS+ KLLP ARL +    E + ++
Sbjct: 361 NPSSISPSYGLAENCTFVSTAWRNNDNSGNSSFRHLPSHNKLLPSARLANEEEEEDMNII 420

Query: 421 VVNGETGEMVDDGVEGEIWISSPSNASGYLGHPLLTRDTFHCKLSNKSSHKFVRTGDRGV 480
           VVN +T E V+D +EGEIW+SSPSNASGYLGHP LT+D F  +LSNK    FVRTGDRG+
Sbjct: 421 VVNEDTHEPVEDEIEGEIWVSSPSNASGYLGHPFLTQDIFKGRLSNKVGRCFVRTGDRGI 480

Query: 481 IKGADRFLFVIGRCSDVITLTNNQEIHPHYIESIAYNNFSAYLRGGCLAAIKVSDTIAVV 540
           +KGA+RFLFV GRC DV+ L N Q++HPHYIE+ AYN     +RGGCLAA  +S  I +V
Sbjct: 481 VKGAERFLFVTGRCLDVVKLPNGQDMHPHYIETTAYNTCPQLIRGGCLAAFDISRMIVLV 540

Query: 541 AEMQREDKNDAETLRQICEGIRKAALIEEGIELGLVVLVKRGNVPKTTSGKVKRWLAKEK 600
           AEMQR +K D + LR ICE +R+  L +E +ELG+VVLVK G+VPKTTSGK++RW AK+ 
Sbjct: 541 AEMQRSEK-DNKILRDICEKMRETVLNQERVELGMVVLVKSGSVPKTTSGKIQRWAAKDN 599

Query: 601 LAGGGMSVLMAVKFGTICGV 618
             GG M VLM +KF    GV
Sbjct: 601 FLGGKMKVLMEMKFDNYHGV 599