CmaCh04G012710.1 (mRNA) Cucurbita maxima (Rimu)

NameCmaCh04G012710.1
TypemRNA
OrganismCucurbita maxima (Cucurbita maxima (Rimu))
DescriptionCalcium-binding EF hand family protein
LocationCma_Chr04 : 6487131 .. 6490269 (+)
Sequence length1492
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ACACGAAGCCCTGCAATTTCCCAAATAAATAAACAATCAATCTTCCAAGAAAATGGGGAAGCTCTCAATTTTCATCTACATATCAATCTTTTCCCTTCTTCTAATCCTTTTCTTTCACACCCCATCACGACGCATCACCCCTCGCCACCGCCGCCTCCGCCTCCGCTCCAACTTCACCTTCAATCAGCCACTCCACCGTCAGCAACAAGACCACTACGCCCCCTTCGACCCTCTCATCGCCAACATCCAACTCCGCCGTGAGGACGAGGAATGGCAGAACCATTCCCTCGGCCCTTCCCAATCCGATGCCGCCGATTCCCACCCAGAGTGGGAGGAGTTCATAAACGATGAGGACAGGTTTAATGTCACCGAAAGATTGTCCTCTCTTTTCCCCAATATCGATGTGAACCCTACCGATGGGTTTATATCTGTAGAGGAATTGACTCGCTGGAACTGGCAGCAGGCCATGAATGCGGCTCTGCATCGGACTGAAAGGGAATTTGAAACTCACGATGAGGATCGTGATGGGTTTGTTTCCTTCGCCGAGTACGAGCCCCCCAGTTGGGTTATGGCCGCTGGTAGATCATCTTCTTTCTTTATACCTCACTTTTCAATTGGATTATACTGTTCTATGTGTTCTTTGATTTTGGGGTCCTCTGAATTTGTATTTTAGTATCTTTTCTCTGTTTCTTTGGCAATTCTATACTATCTGATATGATATCTTTGTTTCCCACTGCCATTTAGGGTTTACTTTATCATGAATTTGTGGCTCATGAAATTCATGAACATTGGAATTTGTGTAGTATTGTACAGAGGAAGTGGAATCCATAACTTCATATAGGCTTAGTTTTCACATATTTTGATGTTTAGTGTCCGAACTGTTCATCAAGGTAATAGTTCTTCCGGCTATGATATTGGCTGGTGGAATGAGGAGCATTTCAATGCATCAGATGCTGATGGGAATGGGGCTTTGGATATGTCTGAATTTAATGAGTAAGCTTTAAAAGTGCCCGTCGTTTATCGATTTCTCTTACTCGATATCGATACTTTCTTGATCTGCTCGTTTTCAGCTTCTTGCATCCTGCTGACAGCAGGAATCCAAAGCTGCTTCTATGGCTGTGTAGAGATGTTGTAAGGTAGATAGATTGCTTCTTAAGTTTTGCCTTCTGTAGTTTGTATTTGTACTCAACTGTTGCTATAATGATGGTTTAACATCATGAAACCGTTACATGAACCGAAAAAACACGGCAAAGAGAACCTTGGATTGACAAGATTCGAACATTTGTTCTAAACTTCTCGGTCTCGAAAGGTTAACGTTCGAATTGATTCAATCTTACTAGATTATTTTGTCGACTGCTGGCTTTATAATGTTATCAATCAGGGAAAGAGATAACAGCAAAGATGGAAAGCTCAGCTTCAGCGAGTTTTCCCCGGCAATTTTTGACTTAGTAAGAAGAGTCGATGAAGATTACGGTTCGTTGAGTTGGGATGATGATCAAATGGAGGCACGGGCAAAAAAGATGTTCTTAGAGCTTGACAAGGACGAAGATGGGTAAACATTGTTCCTATGTGTGCCTTTTTATTGTAAATAATTCACGTTAGTTAGAGAGGGGAACGAAGCATTATTTATAAGAGTGTGGAAACCTCTCCTTAGCAGACGCGTTTTAAAACCTTGAAGGGTAGCCCAATAGGGAAAGCCCAAAGAGGACAATATCTACTAGTGGTAGGCTTGGACGGTTACAATATTATTCTCGGCTTCTTTGCAGGTATCTTTCAGCAACAGAACTGCTGCCTGTTATAGGAAAGATCCATCCATCAGAAGCTTACTATGCAAAACAACAAGCAGAATACATCATCTCTCAGGTACTTTTAGTTCATAAAACTTGGCTCTGCTGCTTTCATGTTGTTTCTGAAGCGTCTTACAGTCCTAAGGGGTTTAACCAATTCACAAAATTATGCATAACAAAAATCGATTTCTAGTAGTGATTCAGTGTAAGATGTTCTTAATTTACTTTCAACGAGGGGTCGGGATCACCGATGTGAGATCCCACCTCGGTTGGAGAAAGGAACAAAACATTCTTTATAAGGGTGGAAGACACGTTTTAAAAACCTTGAGGAAAAGCTCAAAGAGAACAATATCTGTTAGTGATGGGTTTGGGCTGTTACAGATGGTATCGGAGCCAGCACACCAAGCGGTGTGCCAGCAAGGACACTGAGTTCCGAAGGGGGTGGATTGTGAGATCCCACATCGGTTGAAGAGGGAACAGAGCATTCTTTATAAGGGTGTGGAAACTTCTCCCTAGCAGACACGTTTTAAAAATCTTGAGGGAAAGCCCGAAAGAGAGTCAAAAGAGAACAATGTCTGCTATCTGTGAGTTTGGTTGTTACAGTTGGTGTACCTTAACGAGGGGTTGGAAAATATTCAGCCACGGAAAGCTCGATTGATTTATAATCTCGTATCTTCTTCACATGCTACATTCTTAGGCTCACGAGATAGAATGATTTCAATCGTTTTGAAGGCCTGAAAAACAGATTCTTGGCATTGTGAATTGCAGGCAGATAGTGATAATGATGAACTCCTAACTTTGAACGACATGATCGACAACCCGTTTGTGTTCTATAGCTCGGTTTTTAGTGAATACGAGATGGATTACTATCCGTACCACGATGAATTTCGTTAGCTGAATACTGTAAGAACAGCCAAGAAAGTTACTTGTAATCTTTACATCTTTCATCCTCTTCACATCTTGTTGATTATTCACAACTTCTTTCATGGCTTGCAGGTTGTTTAAAGAAAACAGAATATGCGACAAATGAAAACGACGTCGTGAAAGCTCGACAATCTTCGAAGAGATTTTGCTTTAGAAATAATATTAGTTATAGGTGATTCTTTTGGATTCCTCGTCAAATTTTAAAAATAATTAATAAACGTAATAGTATTTTAGTAATTGATAAAAATAAATTATTAAAAAAATATTACTCTCAAATAAGATAGTTTTAAAATAATTAAAATTTAATATTAGCTGTTATTTTGATTAAAATTATAGGAGTATGACATTTGGTCAATGTTTTATTTTTCTTCATTATTGTATACAAGAATATAATTTATTATAATTCTAAGATCGTTTCATCTCTATTA

mRNA sequence

ACACGAAGCCCTGCAATTTCCCAAATAAATAAACAATCAATCTTCCAAGAAAATGGGGAAGCTCTCAATTTTCATCTACATATCAATCTTTTCCCTTCTTCTAATCCTTTTCTTTCACACCCCATCACGACGCATCACCCCTCGCCACCGCCGCCTCCGCCTCCGCTCCAACTTCACCTTCAATCAGCCACTCCACCGTCAGCAACAAGACCACTACGCCCCCTTCGACCCTCTCATCGCCAACATCCAACTCCGCCGTGAGGACGAGGAATGGCAGAACCATTCCCTCGGCCCTTCCCAATCCGATGCCGCCGATTCCCACCCAGAGTGGGAGGAGTTCATAAACGATGAGGACAGGTTTAATGTCACCGAAAGATTGTCCTCTCTTTTCCCCAATATCGATGTGAACCCTACCGATGGGTTTATATCTGTAGAGGAATTGACTCGCTGGAACTGGCAGCAGGCCATGAATGCGGCTCTGCATCGGACTGAAAGGGAATTTGAAACTCACGATGAGGATCGTGATGGGTTTGTTTCCTTCGCCGAGTACGAGCCCCCCAGTTGGGTTATGGCCGCTGGTAATAGTTCTTCCGGCTATGATATTGGCTGGTGGAATGAGGAGCATTTCAATGCATCAGATGCTGATGGGAATGGGGCTTTGGATATGTCTGAATTTAATGACTTCTTGCATCCTGCTGACAGCAGGAATCCAAAGCTGCTTCTATGGCTGTGTAGAGATGTTGTAAGGGAAAGAGATAACAGCAAAGATGGAAAGCTCAGCTTCAGCGAGTTTTCCCCGGCAATTTTTGACTTAGTAAGAAGAGTCGATGAAGATTACGGTTCGTTGAGTTGGGATGATGATCAAATGGAGGCACGGGCAAAAAAGATGTTCTTAGAGCTTGACAAGGACGAAGATGGGTATCTTTCAGCAACAGAACTGCTGCCTGTTATAGGAAAGATCCATCCATCAGAAGCTTACTATGCAAAACAACAAGCAGAATACATCATCTCTCAGGCAGATAGTGATAATGATGAACTCCTAACTTTGAACGACATGATCGACAACCCGTTTGTGTTCTATAGCTCGGTTTTTAGTGAATACGAGATGGATTACTATCCGTACCACGATGAATTTCGTTGTTTAAAGAAAACAGAATATGCGACAAATGAAAACGACGTCGTGAAAGCTCGACAATCTTCGAAGAGATTTTGCTTTAGAAATAATATTAGTTATAGGTGATTCTTTTGGATTCCTCGTCAAATTTTAAAAATAATTAATAAACGTAATAGTATTTTAGTAATTGATAAAAATAAATTATTAAAAAAATATTACTCTCAAATAAGATAGTTTTAAAATAATTAAAATTTAATATTAGCTGTTATTTTGATTAAAATTATAGGAGTATGACATTTGGTCAATGTTTTATTTTTCTTCATTATTGTATACAAGAATATAATTTATTATAATTCTAAGATCGTTTCATCTCTATTA

Coding sequence (CDS)

ATGGGGAAGCTCTCAATTTTCATCTACATATCAATCTTTTCCCTTCTTCTAATCCTTTTCTTTCACACCCCATCACGACGCATCACCCCTCGCCACCGCCGCCTCCGCCTCCGCTCCAACTTCACCTTCAATCAGCCACTCCACCGTCAGCAACAAGACCACTACGCCCCCTTCGACCCTCTCATCGCCAACATCCAACTCCGCCGTGAGGACGAGGAATGGCAGAACCATTCCCTCGGCCCTTCCCAATCCGATGCCGCCGATTCCCACCCAGAGTGGGAGGAGTTCATAAACGATGAGGACAGGTTTAATGTCACCGAAAGATTGTCCTCTCTTTTCCCCAATATCGATGTGAACCCTACCGATGGGTTTATATCTGTAGAGGAATTGACTCGCTGGAACTGGCAGCAGGCCATGAATGCGGCTCTGCATCGGACTGAAAGGGAATTTGAAACTCACGATGAGGATCGTGATGGGTTTGTTTCCTTCGCCGAGTACGAGCCCCCCAGTTGGGTTATGGCCGCTGGTAATAGTTCTTCCGGCTATGATATTGGCTGGTGGAATGAGGAGCATTTCAATGCATCAGATGCTGATGGGAATGGGGCTTTGGATATGTCTGAATTTAATGACTTCTTGCATCCTGCTGACAGCAGGAATCCAAAGCTGCTTCTATGGCTGTGTAGAGATGTTGTAAGGGAAAGAGATAACAGCAAAGATGGAAAGCTCAGCTTCAGCGAGTTTTCCCCGGCAATTTTTGACTTAGTAAGAAGAGTCGATGAAGATTACGGTTCGTTGAGTTGGGATGATGATCAAATGGAGGCACGGGCAAAAAAGATGTTCTTAGAGCTTGACAAGGACGAAGATGGGTATCTTTCAGCAACAGAACTGCTGCCTGTTATAGGAAAGATCCATCCATCAGAAGCTTACTATGCAAAACAACAAGCAGAATACATCATCTCTCAGGCAGATAGTGATAATGATGAACTCCTAACTTTGAACGACATGATCGACAACCCGTTTGTGTTCTATAGCTCGGTTTTTAGTGAATACGAGATGGATTACTATCCGTACCACGATGAATTTCGTTGTTTAAAGAAAACAGAATATGCGACAAATGAAAACGACGTCGTGAAAGCTCGACAATCTTCGAAGAGATTTTGCTTTAGAAATAATATTAGTTATAGGTGA

Protein sequence

MGKLSIFIYISIFSLLLILFFHTPSRRITPRHRRLRLRSNFTFNQPLHRQQQDHYAPFDPLIANIQLRREDEEWQNHSLGPSQSDAADSHPEWEEFINDEDRFNVTERLSSLFPNIDVNPTDGFISVEELTRWNWQQAMNAALHRTEREFETHDEDRDGFVSFAEYEPPSWVMAAGNSSSGYDIGWWNEEHFNASDADGNGALDMSEFNDFLHPADSRNPKLLLWLCRDVVRERDNSKDGKLSFSEFSPAIFDLVRRVDEDYGSLSWDDDQMEARAKKMFLELDKDEDGYLSATELLPVIGKIHPSEAYYAKQQAEYIISQADSDNDELLTLNDMIDNPFVFYSSVFSEYEMDYYPYHDEFRCLKKTEYATNENDVVKARQSSKRFCFRNNISYR
BLAST of CmaCh04G012710.1 vs. Swiss-Prot
Match: CAB45_XENLA (45 kDa calcium-binding protein OS=Xenopus laevis GN=sdf4 PE=2 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 1.6e-14
Identity = 77/290 (26.55%), Postives = 126/290 (43.45%), Query Frame = 1

Query: 92  EWEEFINDEDRFNVTERLSSLFPNIDVNPTDGFISVEELTRW---NWQQAMNAALHRTER 151
           E EEF  D +      +L+++F  +D N  D  IS  E+ RW     ++    A++  + 
Sbjct: 84  ELEEFDEDSEPRRNRRKLAAIFAKVDRNE-DKQISASEMQRWIMEKTEEHFQEAVNENKL 143

Query: 152 EFETHDEDRDGFVSFAEYEPPSWVMAAGNSSSGYDIGWWNEE-----------------H 211
            F   D D DG VS+ EY+         N     +    NE+                  
Sbjct: 144 HFRAVDPDGDGHVSWDEYKIKFLASKGFNEKEVAEKLKNNEDLKIDEETQEVLDNLKDRW 203

Query: 212 FNASDADGNGALDMSEFNDFLHPADSRNPKLLLWLCRDVVRERDNSKDGKLSFSEFSPAI 271
           F A +   +  L+  EF  FLHP  SR   +L ++ ++++R+ D   D KL+ SEF    
Sbjct: 204 FQADNPPPDQLLNEEEFLSFLHPEHSRG--MLKFMVKEIIRDLDQDGDKKLTLSEFISLP 263

Query: 272 FDLVRRVDEDYGSLSWDDDQMEARAKKMFLELDKDEDGYLSATELLPVIGKIHPSEAYYA 331
              V    E+  +   DDD +  R K+    +D + DG ++  EL   +    P   Y A
Sbjct: 264 VGTV----ENQQAQDIDDDWVRDRKKEYEEVIDANHDGIVTMEELEEYMD---PMNEYNA 323

Query: 332 KQQAEYIISQADSDNDELLTLNDMIDNPFVFYSSVFSEYEMDYYPYHDEF 362
             +A+ +I+ AD + D LL+L +++     F  S   +Y  +    H+EF
Sbjct: 324 LNEAKQMIAVADENQDHLLSLEEILKYSEYFTGSKLMDYARN---VHEEF 360

BLAST of CmaCh04G012710.1 vs. Swiss-Prot
Match: RCN2V_CROAD (Reticulocalbin-2 OS=Crotalus adamanteus PE=1 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 2.1e-14
Identity = 69/269 (25.65%), Postives = 129/269 (47.96%), Query Frame = 1

Query: 107 ERLSSLFPNIDVNPTDGFISVEELTRWNWQQAMNAALHRTEREFETHDEDRDGFVSFAEY 166
           +RL  +   IDV+  DGF++  EL+ W      +  +   +++F+ +D+D DG VS+ EY
Sbjct: 53  KRLKVIISRIDVD-LDGFLTEAELSSWIQHSFKSYIIEDAKQQFQHYDKDGDGRVSWEEY 112

Query: 167 EPPSW----------VMAAGNSSSGYDIGWWNEEHFNASDADGNGALDMSEFNDFLHPAD 226
               +           +      S   +   +++ F  ++ DG+  LD  EF  F HP +
Sbjct: 113 NIQMYDRVIDFEEDTTLDDAEEESFRQLHLKDKKRFQKANKDGDSHLDFEEFAAFEHPEE 172

Query: 227 SRNPKLLLWLCRDVVRERDNSKDGKLSFSEFSPAIFDLVR--RVDEDYGSLSWDDDQMEA 286
           +   K   ++ ++ + E D   DG +S  EF   + D  R     ED   +  ++D+ + 
Sbjct: 173 ADYMK--EFVIQESLEEHDKDGDGFVSLQEF---LGDYRRDPAAKEDPEWIVVEEDRFKN 232

Query: 287 RAKKMFLELDKDEDGYLSATELLPVIGKIHPSEAYYAKQQAEYIISQADSDNDELLTLND 346
                  + DKD+DG LS  ELL     + P+    A+++A +++ + D D D  L+ N+
Sbjct: 233 -------DYDKDKDGKLSPKELLT---WVMPNNEGLAQEEAVHLLDEMDLDGDRRLSANE 292

Query: 347 MIDNPFVFYSSVFSEYEMDYYP---YHDE 361
           +++N  +F +S  ++Y    +    YH+E
Sbjct: 293 ILENQDLFLNSEATDYGRQLHDKSFYHEE 305

BLAST of CmaCh04G012710.1 vs. Swiss-Prot
Match: RCN1_HUMAN (Reticulocalbin-1 OS=Homo sapiens GN=RCN1 PE=1 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 4.6e-14
Identity = 67/263 (25.48%), Postives = 117/263 (44.49%), Query Frame = 1

Query: 107 ERLSSLFPNIDVNPTDGFISVEELTRWNWQQAMNAALHRTEREFETHDEDRDGFVSFAEY 166
           ERL  +   ID N  DGF++ EEL  W  +           + ++ +D D+D  +S+ EY
Sbjct: 82  ERLGKIVDRID-NDGDGFVTTEELKTWIKRVQKRYIFDNVAKVWKDYDRDKDDKISWEEY 141

Query: 167 EPPSWVMAAGNSSSGYDIGWWN---------EEHFNASDADGNGALDMSEFNDFLHPADS 226
           +  ++    GN +  +D    +         E  F A+D +G+      EF  FLHP + 
Sbjct: 142 KQATYGYYLGNPAEFHDSSDHHTFKKMLPRDERRFKAADLNGDLTATREEFTAFLHPEEF 201

Query: 227 RNPKLLLWLCRDVVRERDNSKDGKLSFSEFSPAIFDLVRRVDEDYGSLSWDDDQMEARAK 286
            + K ++ L  + + + D + DG +   E+   +F       E    LS  +   E R  
Sbjct: 202 EHMKEIVVL--ETLEDIDKNGDGFVDQDEYIADMFSHEENGPEPDWVLSEREQFNEFRDL 261

Query: 287 KMFLELDKDEDGYLSATELLPVIGKIHPSEAYYAKQQAEYIISQADSDNDELLTLNDMID 346
               +LDKDE           +   I P +  +A+ +A +++ ++D + DE LT  ++++
Sbjct: 262 NKDGKLDKDE-----------IRHWILPQDYDHAQAEARHLVYESDKNKDEKLTKEEILE 321

Query: 347 NPFVFYSSVFSEYEMDYYPYHDE 361
           N  +F  S  + Y  D    HDE
Sbjct: 322 NWNMFVGSQATNYGEDLTKNHDE 330

BLAST of CmaCh04G012710.1 vs. Swiss-Prot
Match: RCN1_MOUSE (Reticulocalbin-1 OS=Mus musculus GN=Rcn1 PE=1 SV=1)

HSP 1 Score: 78.6 bits (192), Expect = 1.7e-13
Identity = 67/263 (25.48%), Postives = 121/263 (46.01%), Query Frame = 1

Query: 107 ERLSSLFPNIDVNPTDGFISVEELTRWNWQQAMNAALHRTEREFETHDEDRDGFVSFAEY 166
           ERL  +   ID +  DG ++ EEL  W  +           + ++ +D D+D  +S+ EY
Sbjct: 76  ERLGKIVDRID-SDGDGLVTTEELKLWIKRVQKRYIYDNVAKVWKDYDRDKDEKISWEEY 135

Query: 167 EPPSWVMAAGNSSSGYDIGWWN---------EEHFNASDADGNGALDMSEFNDFLHPADS 226
           +  ++    GN +  +D    +         E  F ASD DG+      EF  FLHP + 
Sbjct: 136 KQATYGYYLGNPAEFHDSSDHHTFKKMLPRDERRFKASDLDGDLTATREEFTAFLHPEEF 195

Query: 227 RNPKLLLWLCRDVVRERDNSKDGKLSFSEFSPAIFDLVRRVDEDYGSLSWDDDQMEARAK 286
            + K ++ L  + + + D + DG +   E+   +F       ED G    + D + +  +
Sbjct: 196 EHMKEIVVL--ETLEDIDKNGDGFVDQDEYIADMFS-----HEDNGP---EPDWVLSERE 255

Query: 287 KMFLELDKDEDGYLSATELLPVIGKIHPSEAYYAKQQAEYIISQADSDNDELLTLNDMID 346
           +     D ++DG L   E+   I    P +  +A+ +A +++ ++D + DE+LT  +++D
Sbjct: 256 QFNDFRDLNKDGKLDKDEIRHWI---LPQDYDHAQAEARHLVYESDKNKDEMLTKEEILD 315

Query: 347 NPFVFYSSVFSEYEMDYYPYHDE 361
           N  +F  S  + Y  D    HDE
Sbjct: 316 NWNMFVGSQATNYGEDLTKNHDE 324

BLAST of CmaCh04G012710.1 vs. Swiss-Prot
Match: CALUB_DANRE (Calumenin-B OS=Danio rerio GN=calub PE=2 SV=1)

HSP 1 Score: 78.6 bits (192), Expect = 1.7e-13
Identity = 67/261 (25.67%), Postives = 127/261 (48.66%), Query Frame = 1

Query: 107 ERLSSLFPNIDVNPTDGFISVEELTRWNWQQAMNAALHRTEREFETHDEDRDGFVSFAEY 166
           ERL  +   ID +  DGF++ +E+ RW             +R+++ HD + D FVS+ EY
Sbjct: 71  ERLGKIVEKIDEDH-DGFVTADEMKRWIKHAQRRWIYEDVDRQWQAHDLNSDSFVSWEEY 130

Query: 167 EPPSW--VMAAGNSSSGYDIGWW---NEEHFNASDADGNGALDMSEFNDFLHPADSRNPK 226
           +  ++  ++   +   G++       +E  F  +D DG+   +  EF  FLHP +    K
Sbjct: 131 KDATYGYILDEADPEDGFNYRQMMTRDERRFKMADQDGDLRANKEEFTAFLHPEEFDYMK 190

Query: 227 LLLWLCRDVVRERDNSKDGKLSFSEFSPAIFDLVRRVDEDYGSLSWDDDQMEARAKKMFL 286
            ++ L  + + + D + DG +  +E+   I D+  + + D     W   + E      F 
Sbjct: 191 DIVVL--ETMEDIDKNGDGLIDLNEY---IGDMYSQ-NGDSSEPEWVKTERE-----QFT 250

Query: 287 EL-DKDEDGYLSATELLPVIGKIHPSEAYYAKQQAEYIISQADSDNDELLTLNDMIDNPF 346
           E  DK++DG +   E    I    P++  +A+ +A++++ ++D+D D  LT  +++D   
Sbjct: 251 EFRDKNKDGRMDKDETRDWI---LPADYDHAEAEAKHLLYESDADKDGRLTKQEIVDKYD 310

Query: 347 VFYSSVFSEYEMDYYPYHDEF 362
           +F  S  +++  D    HDEF
Sbjct: 311 LFVGSQATDFG-DALVRHDEF 315

BLAST of CmaCh04G012710.1 vs. TrEMBL
Match: A0A0A0KT37_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G579050 PE=4 SV=1)

HSP 1 Score: 575.1 bits (1481), Expect = 6.6e-161
Identity = 281/362 (77.62%), Postives = 314/362 (86.74%), Query Frame = 1

Query: 1   MGKLSIFIYISIFSLLLILFFHTPSRRITPRHRRLRLRSNFTFNQPLHRQQQDHYAPFDP 60
           MGK S+ IYISIFSLLLIL   TP+ R  PRHRRLRLRSNFTFN PL R Q DHY  FDP
Sbjct: 1   MGKFSVLIYISIFSLLLILISQTPTTR--PRHRRLRLRSNFTFNPPLLRHQHDHYISFDP 60

Query: 61  LIANIQLRREDEEWQNHSLGPSQSDAADSHPEWEEFINDEDRFNVTERLSSLFPNIDVNP 120
           LI++I+LRRED+EW   SL      A DSHPEWEEFINDEDRFNVTERL  +FP IDV+P
Sbjct: 61  LISDIELRREDDEWHKQSL--KNLAAHDSHPEWEEFINDEDRFNVTERLFWIFPKIDVDP 120

Query: 121 TDGFISVEELTRWNWQQAMNAALHRTEREFETHDEDRDGFVSFAEYEPPSWVMAAGNSSS 180
           +DGF+S EELTRWN QQAMN AL+RTEREF++HD++RDGFVSFAEYEPPSWV++AGN S 
Sbjct: 121 SDGFVSAEELTRWNLQQAMNEALYRTEREFQSHDDNRDGFVSFAEYEPPSWVLSAGNGSF 180

Query: 181 GYDIGWWNEEHFNASDADGNGALDMSEFNDFLHPADSRNPKLLLWLCRDVVRERDNSKDG 240
           GYDIGWWNEEHFNASDADG+G LD++EFNDFLHPADS++ KLLLWLC DVVRERDN KDG
Sbjct: 181 GYDIGWWNEEHFNASDADGDGVLDLNEFNDFLHPADSKSTKLLLWLCADVVRERDNDKDG 240

Query: 241 KLSFSEFSPAIFDLVRRVDEDYGSLSWDDDQMEARAKKMFLELDKDEDGYLSATELLPVI 300
           KL+FSEF P + DLVRRVDEDY S +W+D++ EA AKKMFLELDKD DGYLS TE+LP+I
Sbjct: 241 KLNFSEFFPKVLDLVRRVDEDYNSSNWEDEEPEALAKKMFLELDKDSDGYLSTTEMLPII 300

Query: 301 GKIHPSEAYYAKQQAEYIISQADSDNDELLTLNDMIDNPFVFYSSVFSEYEMDYYPYHDE 360
           GKIHPSEAYYAKQQAEYIISQADSD+D LLTLNDMI+NP VFYSSVF+E EMDYYPYHDE
Sbjct: 301 GKIHPSEAYYAKQQAEYIISQADSDDDGLLTLNDMIENPSVFYSSVFTEDEMDYYPYHDE 358

Query: 361 FR 363
           FR
Sbjct: 361 FR 358

BLAST of CmaCh04G012710.1 vs. TrEMBL
Match: M5XF48_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa007408mg PE=4 SV=1)

HSP 1 Score: 438.7 bits (1127), Expect = 7.4e-120
Identity = 217/367 (59.13%), Postives = 277/367 (75.48%), Query Frame = 1

Query: 4   LSIFIYISIFSLLLILFFHTPSRRITPRHRRLRLRSNFTFNQPLHRQQQDHYAPFDPLIA 63
           +S+ IYI+I  L+L+L  H+P +  + RHRRL+LRSNFTF  PLH        PFDPL+A
Sbjct: 6   VSVIIYITIAVLILLLISHSPKKGGSQRHRRLKLRSNFTFAPPLHHHNP---IPFDPLVA 65

Query: 64  NIQLRREDEEWQNHSLGPSQS-DAADSHPEWEEF------INDEDRFNVTERLSSLFPNI 123
           +I+ R ED +W+      + S  AA+S PEWE+F      +NDE+RFNVT RL  LFP +
Sbjct: 66  DIERRLEDRKWEQQHFDLNDSAPAAESQPEWEDFMNAEDYLNDEERFNVTNRLLLLFPKV 125

Query: 124 DVNPTDGFISVEELTRWNWQQAMNAALHRTEREFETHDEDRDGFVSFAEYEPPSWVMAAG 183
           DV+P DGFI+ +ELT+WN +QA    LHRT+R+ E HD++RDGFVSFAEY+PPSWV  A 
Sbjct: 126 DVDPADGFITEDELTQWNLKQAQKEVLHRTQRDMELHDKNRDGFVSFAEYQPPSWVQNAD 185

Query: 184 NSSSGYDIGWWNEEHFNASDADGNGALDMSEFNDFLHPADSRNPKLLLWLCRDVVRERDN 243
           N+S GYD+GWW EEHFNASDADG+G L+++EFNDFLHPADS+NPKLL WLC++ VRERD 
Sbjct: 186 NNSFGYDMGWWKEEHFNASDADGDGLLNITEFNDFLHPADSKNPKLLQWLCKEEVRERDT 245

Query: 244 SKDGKLSFSEFSPAIFDLVRRVDED-YGSLSWDDDQMEARAKKMFLELDKDEDGYLSATE 303
            KD K++F EF   +FDLVR  +E+ + S    DD MEA A+ +F +LDKD DGYLS  E
Sbjct: 246 DKDEKINFKEFFHGLFDLVRNYEEENHNSSHQFDDSMEAPARNLFAQLDKDGDGYLSDAE 305

Query: 304 LLPVIGKIHPSEAYYAKQQAEYIISQADSDNDELLTLNDMIDNPFVFYSSVFSEYEMDYY 363
           LLP+IGKIHPSE YYAKQQA+YIISQAD+D D  LTL +MI+NP+VFYS++F++ E + Y
Sbjct: 306 LLPIIGKIHPSEHYYAKQQADYIISQADTDKDGRLTLTEMIENPYVFYSAIFNDDEEEDY 365

BLAST of CmaCh04G012710.1 vs. TrEMBL
Match: B9RGH6_RICCO (Reticulocalbin-2, putative OS=Ricinus communis GN=RCOM_1454090 PE=4 SV=1)

HSP 1 Score: 427.9 bits (1099), Expect = 1.3e-116
Identity = 215/378 (56.88%), Postives = 274/378 (72.49%), Query Frame = 1

Query: 1   MGKLSIFIYISIFSLLLILFFHTPSRRITPRHRRLRLRSNFTFNQPLHRQQQDHYAPFDP 60
           MGKLSI IYI +  L L+L  H+PS+  +  HRRL+LRS+F F+   H  +     PFDP
Sbjct: 1   MGKLSILIYIVLALLFLLLISHSPSKSSSRHHRRLKLRSSFNFSD--HNPRHHEPVPFDP 60

Query: 61  LIANIQLRREDEEWQNHSLGPSQSD------AADSH---PEWEEF------INDEDRFNV 120
           L+A+I+ RRED++W+ H +  S  D      AA  H   PEWE+F      +NDE++FNV
Sbjct: 61  LVADIERRREDKQWEKHYIESSHPDILKDIDAAPGHEPQPEWEDFMDAEDYLNDEEKFNV 120

Query: 121 TERLSSLFPNIDVNPTDGFISVEELTRWNWQQAMNAALHRTEREFETHDEDRDGFVSFAE 180
           T RL  LFP IDV+P DG +S  ELT WN  QA    +HRT+RE E HD++ DG +SF+E
Sbjct: 121 TSRLLLLFPKIDVDPVDGHVSEHELTEWNMDQAKREVMHRTQREVEVHDKNHDGLISFSE 180

Query: 181 YEPPSWVMAAGNSSSGYDIGWWNEEHFNASDADGNGALDMSEFNDFLHPADSRNPKLLLW 240
           YEPPSWV  +  ++ GYD+GWW EEHFNASDADG+G L+++EFNDFLHPADS+NPKLL W
Sbjct: 181 YEPPSWVHNSDQNTFGYDMGWWREEHFNASDADGDGLLNITEFNDFLHPADSKNPKLLQW 240

Query: 241 LCRDVVRERDNSKDGKLSFSEFSPAIFDLVRRVDED-YGSLSWDDDQMEARAKKMFLELD 300
           LC + VRERD+ KDGK++F EF   +FDLVR  DE+ + S    DD +EA AK +F +LD
Sbjct: 241 LCMEEVRERDSDKDGKVNFKEFFHGLFDLVRNYDEESHNSSHPTDDSLEAPAKVLFSQLD 300

Query: 301 KDEDGYLSATELLPVIGKIHPSEAYYAKQQAEYIISQADSDNDELLTLNDMIDNPFVFYS 360
           KD DGYLS  ELLPVIGK+HPSE YYAKQQA+YIISQAD+D D  L+L +MI+NPFVFYS
Sbjct: 301 KDGDGYLSDVELLPVIGKLHPSERYYAKQQADYIISQADTDKDGRLSLTEMIENPFVFYS 360

Query: 361 SVFSEYEMDYYPYHDEFR 363
           ++FS+ + + Y +HDEFR
Sbjct: 361 AIFSDEDDEDYDFHDEFR 376

BLAST of CmaCh04G012710.1 vs. TrEMBL
Match: A0A067FRA4_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g017306mg PE=4 SV=1)

HSP 1 Score: 426.8 bits (1096), Expect = 2.9e-116
Identity = 212/379 (55.94%), Postives = 284/379 (74.93%), Query Frame = 1

Query: 1   MGKLSIFIYISIFSLLLILFFHTPSR-RITPRHRRLRLRSNFTFNQPLHRQQQDHYAPFD 60
           MGK+S+FIY++I  LLL+L   +P++     RHRRL++RS+F F +P H +      PFD
Sbjct: 1   MGKVSLFIYVTIALLLLLLLSKSPNKPHSNRRHRRLKVRSSFNF-RPTHHEP----VPFD 60

Query: 61  PLIANIQLRREDEEWQNHSLGPSQSDAADSH---------PEWEEF------INDEDRFN 120
           PL+A+I+ RRED +W+   +  +  + + +H         PEWE+F      +NDE++FN
Sbjct: 61  PLVADIERRREDRQWEKQYIEHAHHELSHNHDAAPGEEAQPEWEDFMNAEDYLNDEEKFN 120

Query: 121 VTERLSSLFPNIDVNPTDGFISVEELTRWNWQQAMNAALHRTEREFETHDEDRDGFVSFA 180
           VT+RL  LFP IDVNP DG+I+ +ELT WN QQA    +HRT+RE ETHD+++DGFVSFA
Sbjct: 121 VTDRLVLLFPKIDVNPADGYINEDELTDWNMQQAERDVMHRTQREMETHDKNKDGFVSFA 180

Query: 181 EYEPPSWVMAAGNSSSGYDIGWWNEEHFNASDADGNGALDMSEFNDFLHPADSRNPKLLL 240
           EYEPP+WV  + N+S GYD+GWW EEHFNASDADG+G L+++EFNDFLHPAD++NPKL+L
Sbjct: 181 EYEPPTWVRNSDNNSFGYDMGWWKEEHFNASDADGDGLLNLTEFNDFLHPADTKNPKLIL 240

Query: 241 WLCRDVVRERDNSKDGKLSFSEFSPAIFDLVRRV-DEDYGSLSWDDDQMEARAKKMFLEL 300
           WL ++ VRERD+ +DGK++F EF   +FDLVR   DE + S    DD M+A A+++F +L
Sbjct: 241 WLSKEEVRERDSDRDGKVNFKEFFHGLFDLVRNYDDEGHNSSHPSDDTMDAPARQLFGQL 300

Query: 301 DKDEDGYLSATELLPVIGKIHPSEAYYAKQQAEYIISQADSDNDELLTLNDMIDNPFVFY 360
           DKD DGYLS  ELLP+IGK+HPSE YYAKQQA+YIISQAD+D D  LTL +MI+NP+VFY
Sbjct: 301 DKDGDGYLSDVELLPIIGKLHPSERYYAKQQADYIISQADTDKDGRLTLLEMIENPYVFY 360

Query: 361 SSVFSEYEMDYYPYHDEFR 363
           S++F++ + D Y YHDEFR
Sbjct: 361 SAIFTDEDDDDYIYHDEFR 374

BLAST of CmaCh04G012710.1 vs. TrEMBL
Match: V4TM43_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10031861mg PE=4 SV=1)

HSP 1 Score: 426.8 bits (1096), Expect = 2.9e-116
Identity = 212/379 (55.94%), Postives = 284/379 (74.93%), Query Frame = 1

Query: 1   MGKLSIFIYISIFSLLLILFFHTPSR-RITPRHRRLRLRSNFTFNQPLHRQQQDHYAPFD 60
           MGK+S+FIY++I  LLL+L   +P++     RHRRL++RS+F F +P H +      PFD
Sbjct: 1   MGKVSLFIYVTIALLLLLLLSKSPNKPHSNRRHRRLKVRSSFNF-RPAHHEP----VPFD 60

Query: 61  PLIANIQLRREDEEWQNHSLGPSQSDAADSH---------PEWEEF------INDEDRFN 120
           PL+A+I+ RRED +W+   +  +  + + +H         PEWE+F      +NDE++FN
Sbjct: 61  PLVADIERRREDRQWEKQYIEHAHHELSHNHDAAPGEEAQPEWEDFMNAEDYLNDEEKFN 120

Query: 121 VTERLSSLFPNIDVNPTDGFISVEELTRWNWQQAMNAALHRTEREFETHDEDRDGFVSFA 180
           VT+RL  LFP IDVNP DG+I+ +ELT WN QQA    +HRT+RE ETHD+++DGFVSFA
Sbjct: 121 VTDRLVLLFPKIDVNPADGYINEDELTDWNMQQAERDVMHRTQREMETHDKNKDGFVSFA 180

Query: 181 EYEPPSWVMAAGNSSSGYDIGWWNEEHFNASDADGNGALDMSEFNDFLHPADSRNPKLLL 240
           EYEPP+WV  + N+S GYD+GWW EEHFNASDADG+G L+++EFNDFLHPAD++NPKL+L
Sbjct: 181 EYEPPTWVRNSDNNSFGYDMGWWKEEHFNASDADGDGLLNLTEFNDFLHPADTKNPKLIL 240

Query: 241 WLCRDVVRERDNSKDGKLSFSEFSPAIFDLVRRV-DEDYGSLSWDDDQMEARAKKMFLEL 300
           WL ++ VRERD+ +DGK++F EF   +FDLVR   DE + S    DD M+A A+++F +L
Sbjct: 241 WLSKEEVRERDSDRDGKVNFKEFFHGLFDLVRNYDDEGHNSSHPSDDTMDAPARQLFGQL 300

Query: 301 DKDEDGYLSATELLPVIGKIHPSEAYYAKQQAEYIISQADSDNDELLTLNDMIDNPFVFY 360
           DKD DGYLS  ELLP+IGK+HPSE YYAKQQA+YIISQAD+D D  LTL +MI+NP+VFY
Sbjct: 301 DKDGDGYLSDVELLPIIGKLHPSERYYAKQQADYIISQADTDKDGRLTLLEMIENPYVFY 360

Query: 361 SSVFSEYEMDYYPYHDEFR 363
           S++F++ + D Y YHDEFR
Sbjct: 361 SAIFTDEDDDDYIYHDEFR 374

BLAST of CmaCh04G012710.1 vs. TAIR10
Match: AT5G08580.1 (AT5G08580.1 Calcium-binding EF hand family protein)

HSP 1 Score: 391.3 bits (1004), Expect = 6.8e-109
Identity = 199/396 (50.25%), Postives = 269/396 (67.93%), Query Frame = 1

Query: 1   MGKLSIFIYISIFSLLLILFFHTPSRRITP--------RHRRLRLRSNFTFNQPLHRQQQ 60
           M K S+ +YI++  L+L L  ++P ++           +H RL+LRS+F F    H    
Sbjct: 1   MSKASVILYITVGILVLFLVSYSPKKKGDHDHHHGGHNQHHRLKLRSSFNFKPTRHDP-- 60

Query: 61  DHYAPFDPLIANIQLRREDEEWQNHSL------------------GPSQSDAADSHPEWE 120
               PFDPL+A+++ RRED+EW+   +                  G   +   +S PEWE
Sbjct: 61  ---VPFDPLVADMERRREDKEWERQYIEHSHPELVSHSQKETTGGGHEHAPGHESQPEWE 120

Query: 121 EF------INDEDRFNVTERLSSLFPNIDVNPTDGFISVEELTRWNWQQAMNAALHRTER 180
           EF      +NDE++FNVT+RL  LFP IDV+P DGF++  ELT W  Q +    +HRT+R
Sbjct: 121 EFMDAEDYLNDEEKFNVTDRLILLFPKIDVSPADGFMTESELTEWTMQSSAKEVVHRTQR 180

Query: 181 EFETHDEDRDGFVSFAEYEPPSWVMAAGNSSSGYDIGWWNEEHFNASDADGNGALDMSEF 240
           + + HD ++DGF+SF+EYEPPSWV  + N+S GYD+GWW EEHFNASDA+G+G L+++EF
Sbjct: 181 DLDVHDRNKDGFISFSEYEPPSWVRKSDNNSFGYDMGWWKEEHFNASDANGDGLLNLTEF 240

Query: 241 NDFLHPADSRNPKLLLWLCRDVVRERDNSKDGKLSFSEFSPAIFDLVRRVDED-YGSLSW 300
           NDFLHPAD++NPKLLLWLC++ VRERD+ KDGK+SF EF   +FD VR  +ED + S   
Sbjct: 241 NDFLHPADTKNPKLLLWLCKEEVRERDSDKDGKISFEEFFHGLFDTVRNYEEDNHNSTHP 300

Query: 301 DDDQMEARAKKMFLELDKDEDGYLSATELLPVIGKIHPSEAYYAKQQAEYIISQADSDND 360
             D  E  AK++F +LDK++DGYLS  ELLP+I KIHP+E YYAKQQA+YIISQADSD D
Sbjct: 301 YHDLPEGPAKQLFSQLDKNDDGYLSDVELLPIISKIHPTEHYYAKQQADYIISQADSDKD 360

Query: 361 ELLTLNDMIDNPFVFYSSVFSEYEM-DYYPYHDEFR 363
             LTL +MI++P+VFYS++F E +  D Y +HDEFR
Sbjct: 361 RRLTLAEMIEHPYVFYSAIFDEDDTDDDYGFHDEFR 391

BLAST of CmaCh04G012710.1 vs. TAIR10
Match: AT4G27790.1 (AT4G27790.1 Calcium-binding EF hand family protein)

HSP 1 Score: 187.2 bits (474), Expect = 2.0e-47
Identity = 113/363 (31.13%), Postives = 189/363 (52.07%), Query Frame = 1

Query: 1   MGKLSIFIYISIFSLLLILFFHTPSRRITPRHRRLRLRSNFTFNQPLHRQQQDHYAPFDP 60
           M K+ ++  ++   + LIL  H    +       +  R       P+          FDP
Sbjct: 1   MAKVVVYTILTATIIFLILLAHKKQNQTQSIEGLITRRIGRRLEMPV----------FDP 60

Query: 61  LIANIQLRREDEEWQNHSLGPSQSDAADSHPEWEEFINDEDRFNVTERLSSLFPNIDVNP 120
           L+  I+    ++E    ++  ++ +  D    +EE+   E R N T R+  LFP +D +P
Sbjct: 61  LVTRIERLSHEKEAGTKTVEAAKEEKDDM---FEEYFAQERRLNTTMRIKFLFPLLDASP 120

Query: 121 TDGFISVEELTRWNWQQAMNAALHRTEREFETHDEDRDGFVSFAEYEPPSWVMAAGNSSS 180
            DGF+S++EL  W  QQ  +  ++RT +E E  D+D+DG ++F EY P         +  
Sbjct: 121 RDGFVSLKELQTWMMQQTEDNMVYRTAKELELQDKDKDGVITFEEYLPQFSKQDIEKNEK 180

Query: 181 GY-DIGWWNEEHFNASDADGNGALDMSEFNDFLHPADSRNPKLLLWLCRDVVRERDNSKD 240
           G+ + GWW E+ F  SD D NG+LD+ EFN+FLHP DSRN     W+ ++ +   D + D
Sbjct: 181 GHGEAGWWMEQ-FKNSDFDHNGSLDIEEFNNFLHPEDSRNGDTQRWVLKERMTGMDTNGD 240

Query: 241 GKLSFSEFSPAIFDLVRRVDEDYGSLSWDDDQMEARAKKMFLELDKDEDGYLSATELLPV 300
           GKL + EF    +++ +    ++     ++D+     + +F E+D+D+D +L A EL P+
Sbjct: 241 GKLEYKEFVKNAYEMYK----EFAKFEKEEDENVPTPQLLFAEMDRDKDRFLVADELRPI 300

Query: 301 IGKIHPSEAYYAKQQAEYIISQADSDNDELLTLNDMIDNPFVFYSSVFSE--YEMDYYPY 360
           +  + P E  YAK  + ++  +AD D D  L+L +M+ +  VFY +V  E   + DY+  
Sbjct: 301 LQYLQPGEMSYAKFYSTFLCHEADEDKDGKLSLEEMLHHEDVFYKAVHHEDLDDEDYFD- 344

BLAST of CmaCh04G012710.1 vs. TAIR10
Match: AT4G14640.1 (AT4G14640.1 calmodulin 8)

HSP 1 Score: 50.1 bits (118), Expect = 3.7e-06
Identity = 38/148 (25.68%), Postives = 68/148 (45.95%), Query Frame = 1

Query: 189 EEHFNASDADGNGALDMSEFNDFLHPADSRNPKLLLWLCRDVVRERDNSKDGKLSFSEFS 248
           +E F   D DG+G + + E    +   D    +  L    D++ E D+  +G + F+EF 
Sbjct: 15  KEAFCLFDKDGDGCITVEELATVIRSLDQNPTEQEL---HDIITEIDSDSNGTIEFAEFL 74

Query: 249 PAIFDLVRRVDEDYGSLSWDDDQMEARAKKMFLELDKDEDGYLSATELLPVIGKIHPSEA 308
             +   ++  D             E   K+ F   DKD++GY+SA+EL  V+  I+  E 
Sbjct: 75  NLMAKKLQESDA------------EEELKEAFKVFDKDQNGYISASELSHVM--INLGEK 134

Query: 309 YYAKQQAEYIISQADSDNDELLTLNDMI 337
               ++ E +I +AD D D  +  ++ +
Sbjct: 135 -LTDEEVEQMIKEADLDGDGQVNYDEFV 144

BLAST of CmaCh04G012710.1 vs. TAIR10
Match: AT3G22930.1 (AT3G22930.1 calmodulin-like 11)

HSP 1 Score: 50.1 bits (118), Expect = 3.7e-06
Identity = 38/148 (25.68%), Postives = 69/148 (46.62%), Query Frame = 1

Query: 189 EEHFNASDADGNGALDMSEFNDFLHPADSRNPKLLLWLCRDVVRERDNSKDGKLSFSEFS 248
           +E F   D DG+G +   E    +   D    +  L   +D++ E D+  +G + FSEF 
Sbjct: 37  KEAFCLFDKDGDGCITADELATVIRSLDQNPTEQEL---QDMITEIDSDGNGTIEFSEFL 96

Query: 249 PAIFDLVRRVDEDYGSLSWDDDQMEARAKKMFLELDKDEDGYLSATELLPVIGKIHPSEA 308
             + + ++  D D               K+ F   DKD++GY+SA+EL  V+  I+  E 
Sbjct: 97  NLMANQLQETDAD------------EELKEAFKVFDKDQNGYISASELRHVM--INLGEK 156

Query: 309 YYAKQQAEYIISQADSDNDELLTLNDMI 337
               ++ + +I +AD D D  +  ++ +
Sbjct: 157 -LTDEEVDQMIKEADLDGDGQVNYDEFV 166

BLAST of CmaCh04G012710.1 vs. NCBI nr
Match: gi|659090222|ref|XP_008445899.1| (PREDICTED: calumenin-B [Cucumis melo])

HSP 1 Score: 591.3 bits (1523), Expect = 1.3e-165
Identity = 287/362 (79.28%), Postives = 319/362 (88.12%), Query Frame = 1

Query: 1   MGKLSIFIYISIFSLLLILFFHTPSRRITPRHRRLRLRSNFTFNQPLHRQQQDHYAPFDP 60
           MGK S+ IYISIFSLLL+L   TP+    PRHRRLRLRSNFTFN PLHRQQ DHY  FDP
Sbjct: 1   MGKFSVLIYISIFSLLLLLISQTPTT--VPRHRRLRLRSNFTFNPPLHRQQHDHYISFDP 60

Query: 61  LIANIQLRREDEEWQNHSLGPSQSDAADSHPEWEEFINDEDRFNVTERLSSLFPNIDVNP 120
           LI++I+LRRED+EWQ  SL      A DSHPEWEEFINDEDRFNVTERL  +FP IDV+P
Sbjct: 61  LISDIELRREDDEWQKQSL--ENLAAHDSHPEWEEFINDEDRFNVTERLFFIFPKIDVDP 120

Query: 121 TDGFISVEELTRWNWQQAMNAALHRTEREFETHDEDRDGFVSFAEYEPPSWVMAAGNSSS 180
           +DGF+S EELTRWN QQAMN ALHRTEREF++HDE+RDGFVSFAEYEPPSWV+AAGNSS 
Sbjct: 121 SDGFVSAEELTRWNLQQAMNEALHRTEREFQSHDENRDGFVSFAEYEPPSWVLAAGNSSF 180

Query: 181 GYDIGWWNEEHFNASDADGNGALDMSEFNDFLHPADSRNPKLLLWLCRDVVRERDNSKDG 240
           GYDIGWWNEEHFNASDADG+G LD++EFNDFLHPADS++PKLLLWLC DVVRERDN KDG
Sbjct: 181 GYDIGWWNEEHFNASDADGDGVLDLNEFNDFLHPADSKSPKLLLWLCADVVRERDNDKDG 240

Query: 241 KLSFSEFSPAIFDLVRRVDEDYGSLSWDDDQMEARAKKMFLELDKDEDGYLSATELLPVI 300
           KL+FSEF P I DL+RRVDEDY S +W+D++ EA A+KMFLELDKD DG+LS +ELLP+I
Sbjct: 241 KLNFSEFFPKILDLIRRVDEDYNSSNWEDEEPEALARKMFLELDKDSDGFLSTSELLPII 300

Query: 301 GKIHPSEAYYAKQQAEYIISQADSDNDELLTLNDMIDNPFVFYSSVFSEYEMDYYPYHDE 360
           GKIHPSEAYYAKQQAEYIISQADSD+D LLTLNDMI+NPFVFYSSVFSE EMDYYPYHDE
Sbjct: 301 GKIHPSEAYYAKQQAEYIISQADSDDDGLLTLNDMIENPFVFYSSVFSEDEMDYYPYHDE 358

Query: 361 FR 363
           FR
Sbjct: 361 FR 358

BLAST of CmaCh04G012710.1 vs. NCBI nr
Match: gi|778704252|ref|XP_011655504.1| (PREDICTED: calumenin-B [Cucumis sativus])

HSP 1 Score: 575.1 bits (1481), Expect = 9.5e-161
Identity = 281/362 (77.62%), Postives = 314/362 (86.74%), Query Frame = 1

Query: 1   MGKLSIFIYISIFSLLLILFFHTPSRRITPRHRRLRLRSNFTFNQPLHRQQQDHYAPFDP 60
           MGK S+ IYISIFSLLLIL   TP+ R  PRHRRLRLRSNFTFN PL R Q DHY  FDP
Sbjct: 1   MGKFSVLIYISIFSLLLILISQTPTTR--PRHRRLRLRSNFTFNPPLLRHQHDHYISFDP 60

Query: 61  LIANIQLRREDEEWQNHSLGPSQSDAADSHPEWEEFINDEDRFNVTERLSSLFPNIDVNP 120
           LI++I+LRRED+EW   SL      A DSHPEWEEFINDEDRFNVTERL  +FP IDV+P
Sbjct: 61  LISDIELRREDDEWHKQSL--KNLAAHDSHPEWEEFINDEDRFNVTERLFWIFPKIDVDP 120

Query: 121 TDGFISVEELTRWNWQQAMNAALHRTEREFETHDEDRDGFVSFAEYEPPSWVMAAGNSSS 180
           +DGF+S EELTRWN QQAMN AL+RTEREF++HD++RDGFVSFAEYEPPSWV++AGN S 
Sbjct: 121 SDGFVSAEELTRWNLQQAMNEALYRTEREFQSHDDNRDGFVSFAEYEPPSWVLSAGNGSF 180

Query: 181 GYDIGWWNEEHFNASDADGNGALDMSEFNDFLHPADSRNPKLLLWLCRDVVRERDNSKDG 240
           GYDIGWWNEEHFNASDADG+G LD++EFNDFLHPADS++ KLLLWLC DVVRERDN KDG
Sbjct: 181 GYDIGWWNEEHFNASDADGDGVLDLNEFNDFLHPADSKSTKLLLWLCADVVRERDNDKDG 240

Query: 241 KLSFSEFSPAIFDLVRRVDEDYGSLSWDDDQMEARAKKMFLELDKDEDGYLSATELLPVI 300
           KL+FSEF P + DLVRRVDEDY S +W+D++ EA AKKMFLELDKD DGYLS TE+LP+I
Sbjct: 241 KLNFSEFFPKVLDLVRRVDEDYNSSNWEDEEPEALAKKMFLELDKDSDGYLSTTEMLPII 300

Query: 301 GKIHPSEAYYAKQQAEYIISQADSDNDELLTLNDMIDNPFVFYSSVFSEYEMDYYPYHDE 360
           GKIHPSEAYYAKQQAEYIISQADSD+D LLTLNDMI+NP VFYSSVF+E EMDYYPYHDE
Sbjct: 301 GKIHPSEAYYAKQQAEYIISQADSDDDGLLTLNDMIENPSVFYSSVFTEDEMDYYPYHDE 358

Query: 361 FR 363
           FR
Sbjct: 361 FR 358

BLAST of CmaCh04G012710.1 vs. NCBI nr
Match: gi|596186423|ref|XP_007223340.1| (hypothetical protein PRUPE_ppa007408mg [Prunus persica])

HSP 1 Score: 438.7 bits (1127), Expect = 1.1e-119
Identity = 217/367 (59.13%), Postives = 277/367 (75.48%), Query Frame = 1

Query: 4   LSIFIYISIFSLLLILFFHTPSRRITPRHRRLRLRSNFTFNQPLHRQQQDHYAPFDPLIA 63
           +S+ IYI+I  L+L+L  H+P +  + RHRRL+LRSNFTF  PLH        PFDPL+A
Sbjct: 6   VSVIIYITIAVLILLLISHSPKKGGSQRHRRLKLRSNFTFAPPLHHHNP---IPFDPLVA 65

Query: 64  NIQLRREDEEWQNHSLGPSQS-DAADSHPEWEEF------INDEDRFNVTERLSSLFPNI 123
           +I+ R ED +W+      + S  AA+S PEWE+F      +NDE+RFNVT RL  LFP +
Sbjct: 66  DIERRLEDRKWEQQHFDLNDSAPAAESQPEWEDFMNAEDYLNDEERFNVTNRLLLLFPKV 125

Query: 124 DVNPTDGFISVEELTRWNWQQAMNAALHRTEREFETHDEDRDGFVSFAEYEPPSWVMAAG 183
           DV+P DGFI+ +ELT+WN +QA    LHRT+R+ E HD++RDGFVSFAEY+PPSWV  A 
Sbjct: 126 DVDPADGFITEDELTQWNLKQAQKEVLHRTQRDMELHDKNRDGFVSFAEYQPPSWVQNAD 185

Query: 184 NSSSGYDIGWWNEEHFNASDADGNGALDMSEFNDFLHPADSRNPKLLLWLCRDVVRERDN 243
           N+S GYD+GWW EEHFNASDADG+G L+++EFNDFLHPADS+NPKLL WLC++ VRERD 
Sbjct: 186 NNSFGYDMGWWKEEHFNASDADGDGLLNITEFNDFLHPADSKNPKLLQWLCKEEVRERDT 245

Query: 244 SKDGKLSFSEFSPAIFDLVRRVDED-YGSLSWDDDQMEARAKKMFLELDKDEDGYLSATE 303
            KD K++F EF   +FDLVR  +E+ + S    DD MEA A+ +F +LDKD DGYLS  E
Sbjct: 246 DKDEKINFKEFFHGLFDLVRNYEEENHNSSHQFDDSMEAPARNLFAQLDKDGDGYLSDAE 305

Query: 304 LLPVIGKIHPSEAYYAKQQAEYIISQADSDNDELLTLNDMIDNPFVFYSSVFSEYEMDYY 363
           LLP+IGKIHPSE YYAKQQA+YIISQAD+D D  LTL +MI+NP+VFYS++F++ E + Y
Sbjct: 306 LLPIIGKIHPSEHYYAKQQADYIISQADTDKDGRLTLTEMIENPYVFYSAIFNDDEEEDY 365

BLAST of CmaCh04G012710.1 vs. NCBI nr
Match: gi|658048623|ref|XP_008360001.1| (PREDICTED: calumenin-A-like [Malus domestica])

HSP 1 Score: 438.3 bits (1126), Expect = 1.4e-119
Identity = 217/372 (58.33%), Postives = 276/372 (74.19%), Query Frame = 1

Query: 4   LSIFIYISIFSLLLILFFHTPSRRITPRHRRLRLRSNFTFNQPLHRQQQDHYAPFDPLIA 63
           +S+ IY +I  L+L+L  H+P +    RHRRL+LRSNFTF+ PLH        PFDPL+A
Sbjct: 6   VSVIIYXAIAVLVLLLISHSPKKPGNHRHRRLKLRSNFTFSPPLHHHNP---IPFDPLVA 65

Query: 64  NIQLRREDEEWQ----NHSLGPSQ--SDAADSHPEWEEF------INDEDRFNVTERLSS 123
           +I+ RRED +W+    +H+   S   + AA+S PEWE+F      +ND++RFNVT RL  
Sbjct: 66  DIERRREDRKWEQQHFDHAFNASHHSAPAAESQPEWEDFMDAEDYLNDDERFNVTSRLLL 125

Query: 124 LFPNIDVNPTDGFISVEELTRWNWQQAMNAALHRTEREFETHDEDRDGFVSFAEYEPPSW 183
           LFP +DV+P DGF++  ELT+WN  QA    LHRT+R+ E HD++ DGFVSFAEY+PPSW
Sbjct: 126 LFPKVDVDPADGFVTEHELTQWNLNQAQREVLHRTQRDMELHDKNXDGFVSFAEYQPPSW 185

Query: 184 VMAAGNSSSGYDIGWWNEEHFNASDADGNGALDMSEFNDFLHPADSRNPKLLLWLCRDVV 243
              A NSS GYD+GWW EEHFNASDA+G+G L+++EFNDFLHPADS+NPKLL WLCR+ V
Sbjct: 186 AQNADNSSFGYDMGWWKEEHFNASDANGDGLLNLTEFNDFLHPADSKNPKLLTWLCREEV 245

Query: 244 RERDNSKDGKLSFSEFSPAIFDLVRRVD-EDYGSLSWDDDQMEARAKKMFLELDKDEDGY 303
           RERD  KD K++F EF   +FDLVR  D ED+ S    DD ME+ A+ +F +LDKD DGY
Sbjct: 246 RERDTDKDEKINFKEFFHGLFDLVRNYDEEDHNSTHQSDDSMESXARNLFAQLDKDGDGY 305

Query: 304 LSATELLPVIGKIHPSEAYYAKQQAEYIISQADSDNDELLTLNDMIDNPFVFYSSVFSEY 363
           LS  E+LP+IGKIHPSE YYAKQQA+YIISQAD+D D  LTL +MI+NP+VFYS++FS+ 
Sbjct: 306 LSDEEILPIIGKIHPSEHYYAKQQADYIISQADTDKDGRLTLGEMIENPYVFYSAIFSDD 365

BLAST of CmaCh04G012710.1 vs. NCBI nr
Match: gi|645228629|ref|XP_008221087.1| (PREDICTED: calumenin [Prunus mume])

HSP 1 Score: 436.8 bits (1122), Expect = 4.0e-119
Identity = 216/367 (58.86%), Postives = 276/367 (75.20%), Query Frame = 1

Query: 4   LSIFIYISIFSLLLILFFHTPSRRITPRHRRLRLRSNFTFNQPLHRQQQDHYAPFDPLIA 63
           +S+ IYI+I  L+L+L  H+P +  + RHRRL+LRSNFTF  PLH        PFDPL+A
Sbjct: 6   VSVIIYITIAVLILLLISHSPKKGGSQRHRRLKLRSNFTFAPPLHHHNP---IPFDPLVA 65

Query: 64  NIQLRREDEEWQNHSLGPSQS-DAADSHPEWEEF------INDEDRFNVTERLSSLFPNI 123
           +I+ R ED +W+      + S  AA+S PEWE+F      +NDE+RFNVT RL  LFP +
Sbjct: 66  DIERRLEDRKWEQQHFDLNDSAPAAESQPEWEDFMNAEDYLNDEERFNVTNRLLLLFPKV 125

Query: 124 DVNPTDGFISVEELTRWNWQQAMNAALHRTEREFETHDEDRDGFVSFAEYEPPSWVMAAG 183
           DV+P DGFI+ +ELT+WN +QA    LHRT+R+ E HD++RDGFVSFAEY+PPSWV  A 
Sbjct: 126 DVDPADGFITEDELTQWNLKQAQKEVLHRTQRDMELHDKNRDGFVSFAEYQPPSWVQNAD 185

Query: 184 NSSSGYDIGWWNEEHFNASDADGNGALDMSEFNDFLHPADSRNPKLLLWLCRDVVRERDN 243
           N+S GYD+GWW EEHFNASDADG+G L+++EFNDFLHPADS+NPKLL WLC++ VRERD 
Sbjct: 186 NNSFGYDMGWWKEEHFNASDADGDGLLNITEFNDFLHPADSKNPKLLQWLCKEEVRERDT 245

Query: 244 SKDGKLSFSEFSPAIFDLVRRVDED-YGSLSWDDDQMEARAKKMFLELDKDEDGYLSATE 303
            KD K++F EF   +FDLVR  +E+ + S    DD MEA A+ +F +LDKD DGYLS  E
Sbjct: 246 DKDEKINFKEFFHGLFDLVRNYEEENHNSSHQFDDSMEAPARNLFAQLDKDGDGYLSDAE 305

Query: 304 LLPVIGKIHPSEAYYAKQQAEYIISQADSDNDELLTLNDMIDNPFVFYSSVFSEYEMDYY 363
           LLP+IGKIHPSE YYAKQQA+YI SQAD+D D  LTL +MI+NP+VFYS++F++ E + Y
Sbjct: 306 LLPIIGKIHPSEHYYAKQQADYITSQADTDKDGRLTLTEMIENPYVFYSAIFNDDEEEDY 365

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
CAB45_XENLA1.6e-1426.5545 kDa calcium-binding protein OS=Xenopus laevis GN=sdf4 PE=2 SV=1[more]
RCN2V_CROAD2.1e-1425.65Reticulocalbin-2 OS=Crotalus adamanteus PE=1 SV=1[more]
RCN1_HUMAN4.6e-1425.48Reticulocalbin-1 OS=Homo sapiens GN=RCN1 PE=1 SV=1[more]
RCN1_MOUSE1.7e-1325.48Reticulocalbin-1 OS=Mus musculus GN=Rcn1 PE=1 SV=1[more]
CALUB_DANRE1.7e-1325.67Calumenin-B OS=Danio rerio GN=calub PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KT37_CUCSA6.6e-16177.62Uncharacterized protein OS=Cucumis sativus GN=Csa_5G579050 PE=4 SV=1[more]
M5XF48_PRUPE7.4e-12059.13Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa007408mg PE=4 SV=1[more]
B9RGH6_RICCO1.3e-11656.88Reticulocalbin-2, putative OS=Ricinus communis GN=RCOM_1454090 PE=4 SV=1[more]
A0A067FRA4_CITSI2.9e-11655.94Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g017306mg PE=4 SV=1[more]
V4TM43_9ROSI2.9e-11655.94Uncharacterized protein OS=Citrus clementina GN=CICLE_v10031861mg PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G08580.16.8e-10950.25 Calcium-binding EF hand family protein[more]
AT4G27790.12.0e-4731.13 Calcium-binding EF hand family protein[more]
AT4G14640.13.7e-0625.68 calmodulin 8[more]
AT3G22930.13.7e-0625.68 calmodulin-like 11[more]
Match NameE-valueIdentityDescription
gi|659090222|ref|XP_008445899.1|1.3e-16579.28PREDICTED: calumenin-B [Cucumis melo][more]
gi|778704252|ref|XP_011655504.1|9.5e-16177.62PREDICTED: calumenin-B [Cucumis sativus][more]
gi|596186423|ref|XP_007223340.1|1.1e-11959.13hypothetical protein PRUPE_ppa007408mg [Prunus persica][more]
gi|658048623|ref|XP_008360001.1|1.4e-11958.33PREDICTED: calumenin-A-like [Malus domestica][more]
gi|645228629|ref|XP_008221087.1|4.0e-11958.86PREDICTED: calumenin [Prunus mume][more]
The following terms have been associated with this mRNA:
Vocabulary: INTERPRO
TermDefinition
IPR002048EF_hand_dom
IPR011992EF-hand-dom_pair
IPR018247EF_Hand_1_Ca_BS
Vocabulary: Molecular Function
TermDefinition
GO:0005509calcium ion binding
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0044699 single-organism process
cellular_component GO:0044444 cytoplasmic part
cellular_component GO:0012505 endomembrane system
cellular_component GO:0043231 intracellular membrane-bounded organelle
molecular_function GO:0005509 calcium ion binding

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
CmaCh04G012710CmaCh04G012710gene


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
CmaCh04G012710.1CmaCh04G012710.1-proteinpolypeptide


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmaCh04G012710.1.exon.1CmaCh04G012710.1.exon.1exon
CmaCh04G012710.1.exon.2CmaCh04G012710.1.exon.2exon
CmaCh04G012710.1.exon.3CmaCh04G012710.1.exon.3exon
CmaCh04G012710.1.exon.4CmaCh04G012710.1.exon.4exon
CmaCh04G012710.1.exon.5CmaCh04G012710.1.exon.5exon
CmaCh04G012710.1.exon.6CmaCh04G012710.1.exon.6exon
CmaCh04G012710.1.exon.7CmaCh04G012710.1.exon.7exon


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmaCh04G012710.1.five_prime_UTR.1CmaCh04G012710.1.five_prime_UTR.1five_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmaCh04G012710.1.CDS.1CmaCh04G012710.1.CDS.1CDS
CmaCh04G012710.1.CDS.2CmaCh04G012710.1.CDS.2CDS
CmaCh04G012710.1.CDS.3CmaCh04G012710.1.CDS.3CDS
CmaCh04G012710.1.CDS.4CmaCh04G012710.1.CDS.4CDS
CmaCh04G012710.1.CDS.5CmaCh04G012710.1.CDS.5CDS
CmaCh04G012710.1.CDS.6CmaCh04G012710.1.CDS.6CDS
CmaCh04G012710.1.CDS.7CmaCh04G012710.1.CDS.7CDS


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmaCh04G012710.1.three_prime_UTR.1CmaCh04G012710.1.three_prime_UTR.1three_prime_UTR


Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainPFAMPF13202EF-hand_5coord: 191..211
score: 0
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 275..336
score: 6.
IPR002048EF-hand domainSMARTSM00054efh_1coord: 187..215
score: 15.0coord: 226..254
score: 72.0coord: 275..303
score: 0.26coord: 145..176
score:
IPR002048EF-hand domainPROFILEPS50222EF_HAND_2coord: 141..178
score: 6.061coord: 189..218
score: 9.185coord: 271..306
score: 12.198coord: 310..345
score: 5.643coord: 227..257
score: 7
IPR011992EF-hand domain pairGENE3DG3DSA:1.10.238.10coord: 247..285
score: 8.0E-7coord: 286..335
score: 2.3E-7coord: 107..246
score: 8.4
IPR011992EF-hand domain pairunknownSSF47473EF-handcoord: 107..216
score: 1.0E-10coord: 190..348
score: 5.33
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 284..296
score: -coord: 196..208
score: -coord: 235..247
score: -coord: 154..166
scor
NoneNo IPR availablePANTHERPTHR10827RETICULOCALBINcoord: 6..362
score: 9.8
NoneNo IPR availablePANTHERPTHR10827:SF53SUBFAMILY NOT NAMEDcoord: 6..362
score: 9.8