CmaCh03G008670.1 (mRNA) Cucurbita maxima (Rimu) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CmaCh03G008670.1
Type	mRNA
Organism	Cucurbita maxima (Cucurbita maxima (Rimu))
Description	Basic helix loop helix (BHLH) DNA-binding family protein
Location	Cma_Chr03 : 6435098 .. 6436076 (+)
Sequence length	792

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATGGACTGTGCATATCAATACGAGATCACCAATTATCCCCATGATTACACACTCGATGAGCTCAATTTTCCGACCAATTTTCCGGCGACCGAAAAACTCATTACTTCGGTCCAACTTGATTCTATTTCCTCTTCCCAGTTTATTTCCTTTGGGGATTCACCTGAAATTGAGAAGCTTGATTGTGCGATGGGTAAGGTCGAGAGAGGAAAAGATTTGTCGTTCATAATACCCCAAAGTTTGGGTGTGAATCAAAATAGGTATGTAATTGGATATGAAGAAGGCCATGACATTAAGAAGCGTGCAACAATTGGTAGAAATTCAGGAAATGGTCAACAACATGTTATAGCTGAGAGAAGACGAAGAGAGAAGCTAAGCCAAAGCTTTGCTGCTCTTTCAGCTCTAATCCCCGGCCTCAACAAGGTAAAAATTTGCTCATAATATCCAATTGCTATGAACAAAAAAGGGTTAATCTTAGATTTTGAATTCAATGTGGTAAGTTTATGTGAAATATGAAAGGGTTAATATTTTTGGTGACAGACGGACAAGGCTTCAGTGCTTGGGGGAGCTATCAAGTACGTGAAAGAGCTTCAAGAACGTGTCAAATGGGCTGAGGAAGAAGCAGGAGATGAGGAGGGGAGAGTAATTAAATCAGTTCCTAAGATAGAAACAAGGGTTTTGGAGAGGGATGTTCTTGTGAAAATTCATTGCAAGAAACAAAAAGGCTGTTTTTCAAACATATTAACCCAAATACAGAAGCTTAAGCTAACCATTGTGAATAGCTGCGTTTTACCATTTGGGCATTCCAGACTTGATATAACCATTATTGCTCAGGTCAAATTCCTTTCTCTCTATTATCACCAAACATAATGACCTTTCATTAATATTTGCTACCTTGTTGCAGATGGAGGTTGGTTTTTGCATGACAGCCACAGATCTTGGGAACAAGCTAACACAGGCTTTGGTCGACCTCATATAA

mRNA sequence

ATGATGGACTGTGCATATCAATACGAGATCACCAATTATCCCCATGATTACACACTCGATGAGCTCAATTTTCCGACCAATTTTCCGGCGACCGAAAAACTCATTACTTCGGTCCAACTTGATTCTATTTCCTCTTCCCAGTTTATTTCCTTTGGGGATTCACCTGAAATTGAGAAGCTTGATTGTGCGATGGGTAAGGTCGAGAGAGGAAAAGATTTGTCGTTCATAATACCCCAAAGTTTGGGTGTGAATCAAAATAGGTATGTAATTGGATATGAAGAAGGCCATGACATTAAGAAGCGTGCAACAATTGGTAGAAATTCAGGAAATGGTCAACAACATGTTATAGCTGAGAGAAGACGAAGAGAGAAGCTAAGCCAAAGCTTTGCTGCTCTTTCAGCTCTAATCCCCGGCCTCAACAAGACGGACAAGGCTTCAGTGCTTGGGGGAGCTATCAAGTACGTGAAAGAGCTTCAAGAACGTGTCAAATGGGCTGAGGAAGAAGCAGGAGATGAGGAGGGGAGAGTAATTAAATCAGTTCCTAAGATAGAAACAAGGGTTTTGGAGAGGGATGTTCTTGTGAAAATTCATTGCAAGAAACAAAAAGGCTGTTTTTCAAACATATTAACCCAAATACAGAAGCTTAAGCTAACCATTGTGAATAGCTGCGTTTTACCATTTGGGCATTCCAGACTTGATATAACCATTATTGCTCAGATGGAGGTTGGTTTTTGCATGACAGCCACAGATCTTGGGAACAAGCTAACACAGGCTTTGGTCGACCTCATATAA

Coding sequence (CDS)

ATGATGGACTGTGCATATCAATACGAGATCACCAATTATCCCCATGATTACACACTCGATGAGCTCAATTTTCCGACCAATTTTCCGGCGACCGAAAAACTCATTACTTCGGTCCAACTTGATTCTATTTCCTCTTCCCAGTTTATTTCCTTTGGGGATTCACCTGAAATTGAGAAGCTTGATTGTGCGATGGGTAAGGTCGAGAGAGGAAAAGATTTGTCGTTCATAATACCCCAAAGTTTGGGTGTGAATCAAAATAGGTATGTAATTGGATATGAAGAAGGCCATGACATTAAGAAGCGTGCAACAATTGGTAGAAATTCAGGAAATGGTCAACAACATGTTATAGCTGAGAGAAGACGAAGAGAGAAGCTAAGCCAAAGCTTTGCTGCTCTTTCAGCTCTAATCCCCGGCCTCAACAAGACGGACAAGGCTTCAGTGCTTGGGGGAGCTATCAAGTACGTGAAAGAGCTTCAAGAACGTGTCAAATGGGCTGAGGAAGAAGCAGGAGATGAGGAGGGGAGAGTAATTAAATCAGTTCCTAAGATAGAAACAAGGGTTTTGGAGAGGGATGTTCTTGTGAAAATTCATTGCAAGAAACAAAAAGGCTGTTTTTCAAACATATTAACCCAAATACAGAAGCTTAAGCTAACCATTGTGAATAGCTGCGTTTTACCATTTGGGCATTCCAGACTTGATATAACCATTATTGCTCAGATGGAGGTTGGTTTTTGCATGACAGCCACAGATCTTGGGAACAAGCTAACACAGGCTTTGGTCGACCTCATATAA

Protein sequence

MMDCAYQYEITNYPHDYTLDELNFPTNFPATEKLITSVQLDSISSSQFISFGDSPEIEKLDCAMGKVERGKDLSFIIPQSLGVNQNRYVIGYEEGHDIKKRATIGRNSGNGQQHVIAERRRREKLSQSFAALSALIPGLNKTDKASVLGGAIKYVKELQERVKWAEEEAGDEEGRVIKSVPKIETRVLERDVLVKIHCKKQKGCFSNILTQIQKLKLTIVNSCVLPFGHSRLDITIIAQMEVGFCMTATDLGNKLTQALVDLI

Homology

[Close]

BLAST of CmaCh03G008670.1 vs. Swiss-Prot
Match: BH025_ARATH (Transcription factor bHLH25 OS=Arabidopsis thaliana GN=BHLH25 PE=2 SV=2)

HSP 1 Score: 137.5 bits (345), Expect = 2.1e-31
Identity = 86/192 (44.79%), Postives = 111/192 (57.81%), Query Frame = 1


Query: 99  KKRATIGRNSGNGQQHVIAERRRREKLSQSFAALSALIPGLNKTDKASVLGGAIKYVKEL 158
           K+     RN  N Q H+IAER+RREKL+Q F ALSAL+PGL K DKASVLG A+K++K L
Sbjct: 138 KRAQPFSRNQSNAQDHIIAERKRREKLTQRFVALSALVPGLKKMDKASVLGDALKHIKYL 197

Query: 159 QERVKWAEEEAGD---------------------------EEGRVIKSVPKIETRVLERD 218
           QERV   EE+  +                           E+G     +P+IE R  + D
Sbjct: 198 QERVGELEEQKKERRLESMVLVKKSKLILDDNNQSFSSSCEDGFSDLDLPEIEVRFSDED 257

Query: 219 VLVKIHCKKQKGCFSNILTQIQKLKLTIVNSCVLPFGHSRLDITIIAQMEVGFCMTATDL 264
           VL+KI C+KQKG  + I+ +I+KL + I NS VL FG + LDITIIA+ E  F MT  D+
Sbjct: 258 VLIKILCEKQKGHLAKIMAEIEKLHILITNSSVLNFGPT-LDITIIAKKESDFDMTLMDV 317

[Close]

BLAST of CmaCh03G008670.1 vs. Swiss-Prot
Match: BH018_ARATH (Transcription factor bHLH18 OS=Arabidopsis thaliana GN=BHLH18 PE=2 SV=1)

HSP 1 Score: 127.5 bits (319), Expect = 2.2e-28
Identity = 84/194 (43.30%), Postives = 110/194 (56.70%), Query Frame = 1


Query: 99  KKRATIGRNSGNGQQHVIAERRRREKLSQSFAALSALIPGLNKTDKASVLGGAIKYVKEL 158
           K+  ++ R+  N Q H++AER+RREKL+Q F ALSALIPGL K DKASVLG AIK++K L
Sbjct: 112 KRAQSLTRSQSNAQDHILAERKRREKLTQRFVALSALIPGLKKMDKASVLGDAIKHIKYL 171

Query: 159 QERVKWAEEEAGDEEGRVIKSV------------------------------PKIETRVL 218
           QE VK  EE+  ++    +  V                              P+IE RV 
Sbjct: 172 QESVKEYEEQKKEKTMESVVLVKKSSLVLDENHQPSSSSSSDGNRNSSSSNLPEIEVRVS 231

Query: 219 ERDVLVKIHCKKQKGCFSNILTQIQKLKLTIVNSCVLPFGHSRLDITIIAQMEVGFCMTA 263
            +DVL+KI C+KQKG    I+ +I+KL L+I NS VLPFG +  DI+IIAQ    F M  
Sbjct: 232 GKDVLIKILCEKQKGNVIKIMGEIEKLGLSITNSNVLPFGPT-FDISIIAQKNNNFDMKI 291

[Close]

BLAST of CmaCh03G008670.1 vs. Swiss-Prot
Match: BH019_ARATH (Transcription factor bHLH19 OS=Arabidopsis thaliana GN=BHLH19 PE=2 SV=1)

HSP 1 Score: 124.4 bits (311), Expect = 1.8e-27
Identity = 74/178 (41.57%), Postives = 107/178 (60.11%), Query Frame = 1


Query: 112 QQHVIAERRRREKLSQSFAALSALIPGLNKTDKASVLGGAIKYVKELQERVKWAEEEAG- 171
           ++HV+AER+RREKLS+ F ALSAL+PGL K DK ++L  AI  +K+LQE+++  +EE   
Sbjct: 118 KEHVLAERKRREKLSEKFIALSALLPGLKKADKVTILDDAISRMKQLQEQLRTLKEEKEA 177

Query: 172 -----------------DEEGRVI----------KSVPKIETRVLERDVLVKIHCKKQKG 231
                            DEE  +           +++P+IE ++ + D+L++I C+K KG
Sbjct: 178 TRQMESMILVKKSKVFFDEEPNLSCSPSVHIEFDQALPEIEAKISQNDILIRILCEKSKG 237

Query: 232 CFSNILTQIQKLKLTIVNSCVLPFGHSRLDITIIAQMEVGFCMT-ATDLGNKLTQALV 261
           C  NIL  I+  +L I NS VLPFG S LDIT++AQM+  F M+   DL   L  A+V
Sbjct: 238 CMINILNTIENFQLRIENSIVLPFGDSTLDITVLAQMDKDFSMSILKDLVRNLRLAMV 295

[Close]

BLAST of CmaCh03G008670.1 vs. Swiss-Prot
Match: BH020_ARATH (Transcription factor NAI1 OS=Arabidopsis thaliana GN=NAI1 PE=2 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 3.4e-13
Identity = 36/93 (38.71%), Postives = 59/93 (63.44%), Query Frame = 1


Query: 169 AGDEEGRVIKSVPKIETRVLERDVLVKIHCKKQKGCFSNILTQIQKLKLTIVNSCVLPFG 228
           + DE     +++P IE RV +RD+L+++HC+K KGC   IL+ ++K +L +VNS  LPFG
Sbjct: 228 SSDEVSIFKQTMPMIEARVSDRDLLIRVHCEKNKGCMIKILSSLEKFRLEVVNSFTLPFG 287

Query: 229 HSRLDITIIAQMEVGFCMTATDLGNKLTQALVD 262
           +S L ITI+ +M+  F     ++   +  AL +
Sbjct: 288 NSTLVITILTKMDNKFSRPVEEVVKNIRVALAE 320

[Close]

BLAST of CmaCh03G008670.1 vs. Swiss-Prot
Match: AIB_ARATH (Transcription factor ABA-INDUCIBLE bHLH-TYPE OS=Arabidopsis thaliana GN=AIB PE=2 SV=2)

HSP 1 Score: 75.9 bits (185), Expect = 7.5e-13
Identity = 43/111 (38.74%), Postives = 69/111 (62.16%), Query Frame = 1


Query: 97  DIKKRATIGRNSGNGQQ----HVIAERRRREKLSQSFAALSALIPGLNKTDKASVLGGAI 156
           D K+    GR   NG++    HV AER+RREKL+Q F AL +++P ++K DKAS+LG AI
Sbjct: 375 DEKRPRKRGRKPANGREEPLNHVEAERQRREKLNQRFYALRSVVPNISKMDKASLLGDAI 434

Query: 157 KYVKELQERVKWAEEE-------AGDEEGRVIKSVPKIETRVLERDVLVKI 197
            Y+KELQE+VK  E+E         +     ++  P+++ + +  +V+V++
Sbjct: 435 SYIKELQEKVKIMEDERVGTDKSLSESNTITVEESPEVDIQAMNEEVVVRV 485

[Close]

BLAST of CmaCh03G008670.1 vs. TrEMBL
Match: A0A0A0KLM8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G497120 PE=4 SV=1)

HSP 1 Score: 236.5 bits (602), Expect = 3.7e-59
Identity = 157/323 (48.61%), Postives = 192/323 (59.44%), Query Frame = 1


Query: 1   MMDCAYQYE-ITNYPHDYTLDELNFPTNFPATEKLITSVQLDSISSSQFISFGDSPEIEK 60
           MM+C+Y+YE +  YPHDYTLDELNFP    A EK+      +S SS Q ISFGD      
Sbjct: 1   MMECSYEYERMKKYPHDYTLDELNFP----AMEKVN-----NSRSSGQLISFGDYS---- 60

Query: 61  LDCAMGKVERGKDLSFIIPQSLGVNQNRYVIGYEEG-HDIKKR--ATI-------GRNSG 120
                G++E  K            +    +IG+EE  H+IKK   +TI         ++ 
Sbjct: 61  -----GEIENYK-----------FDDYGNLIGFEEEEHEIKKDYCSTIIMGQLPNHNHNN 120

Query: 121 NGQQHVIAERRRREKLSQSFAALSALIPGLNK------------------TDKASVLGGA 180
           N  +HVIAERRRREK+ Q+F ALSALIPGL K                   DKASVLGGA
Sbjct: 121 NNSEHVIAERRRREKIRQNFIALSALIPGLIKDHDGHTVLQVEVSVEGLKADKASVLGGA 180

Query: 181 IKYVKELQERVKWAEEEAGDEEGRVIK-------------------------------SV 240
           IK+VKELQER+KWAEE+   E+ RVIK                               SV
Sbjct: 181 IKFVKELQERLKWAEEKE-KEQKRVIKSVVFVKTINLDSDFDNETFSLDENGGRFSVRSV 240

Query: 241 PKIETRVLERDVLVKIHCKKQKGCFSNILTQIQKLKLTIVNSCVLPFGHSRLDITIIAQM 264
           P IETRVLE+DVLV+IHCKK KGC+++I+++I+KLKLTIVNSCV PFG SRLDITIIA+M
Sbjct: 241 PTIETRVLEKDVLVRIHCKKHKGCYTSIVSEIEKLKLTIVNSCVFPFGQSRLDITIIAEM 293

[Close]

BLAST of CmaCh03G008670.1 vs. TrEMBL
Match: M5WL55_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa017640mg PE=4 SV=1)

HSP 1 Score: 171.8 bits (434), Expect = 1.1e-39
Identity = 98/194 (50.52%), Postives = 125/194 (64.43%), Query Frame = 1


Query: 93  EEGHDIKKRATIGRNSGNGQQHVIAERRRREKLSQSFAALSALIPGLNKTDKASVLGGAI 152
           + G  IK+ AT+ R+  + Q HV+AER+RREKLSQ F ALSAL+PGL K DKASVLG AI
Sbjct: 151 KHGQGIKRAATVTRSPLHAQDHVLAERKRREKLSQRFIALSALVPGLKKMDKASVLGDAI 210

Query: 153 KYVKELQERVKWAEEEA------------------------GDE--EGRVIKSVPKIETR 212
           KYVK LQER K  EE+A                         DE  E    + +P+IE R
Sbjct: 211 KYVKHLQERTKMLEEKAVKKTVEAVVFVKRTQYSADDDISSSDENFESSSDQPLPEIEAR 270

Query: 213 VLERDVLVKIHCKKQKGCFSNILTQIQKLKLTIVNSCVLPFGHSRLDITIIAQMEVGFCM 261
           V +++VL+++HC+K KGC + IL++I+ L LTIVNS VLPFG+S LDIT+IAQM+  F M
Sbjct: 271 VSDKEVLIRVHCEKTKGCLAKILSEIESLDLTIVNSSVLPFGNSTLDITVIAQMDAEFSM 330

[Close]

BLAST of CmaCh03G008670.1 vs. TrEMBL
Match: A0A0B0PCU7_GOSAR (Transcription factor bHLH19-like protein OS=Gossypium arboreum GN=F383_28381 PE=4 SV=1)

HSP 1 Score: 165.6 bits (418), Expect = 8.0e-38
Identity = 116/300 (38.67%), Postives = 162/300 (54.00%), Query Frame = 1


Query: 7   QYEITNYPHDYTLDELNFPTNFPATEKLITSVQLDSISSSQFISFGDSPEIEKLDCAMGK 66
           QY++ N P DY+  ++NF +   +T    T         + FI    S E++     M +
Sbjct: 22  QYQVIN-PIDYSFHDINFHSENYSTNPYYTI-------GNSFIEA--SHEVDDFKRPMKQ 81

Query: 67  VERGKDLSFII-------PQSLGVNQNRYVIGYEEG-------HDIKKRATIG---RNSG 126
           ++     S I        P+    + + ++I ++         +D+K +  +G   R   
Sbjct: 82  LKTNSWNSCITENNIPPPPKPSSSSSSSHLISFDNSNSYYAVDYDVKPKNEVGSFTRTPL 141

Query: 127 NGQQHVIAERRRREKLSQSFAALSALIPGLNKTDKASVLGGAIKYVKELQERVKWAEEE- 186
           + Q HV+AER+RR KLSQSF +LSALIPGL KTDKASVLG AI Y+K+LQ+RV   EE+ 
Sbjct: 142 HAQDHVLAERKRRAKLSQSFISLSALIPGLKKTDKASVLGDAITYLKKLQDRVNVLEEQV 201

Query: 187 -----------------AGDEEGRVIKSV--------PKIETRVLERDVLVKIHCKKQKG 246
                            A DE     +SV        P+IE RV ++DVL++IHCK  KG
Sbjct: 202 AKKTMESGIFVRKTQIYADDETSSSDESVHGQSNNPFPEIEARVSDKDVLIRIHCKHNKG 261

Query: 247 CFSNILTQIQKLKLTIVNSCVLPFGHSRLDITIIAQMEVGFCMTATDLGNKLTQALVDLI 264
           C SNI+ +++KL L ++NS VLPFG + LDITI AQME  F MTA DL   L  AL+  I
Sbjct: 262 CISNIINEVEKLHLFVLNSHVLPFGQATLDITITAQMEAEFSMTAKDLVKSLRLALLKFI 311

[Close]

BLAST of CmaCh03G008670.1 vs. TrEMBL
Match: A0A067FXL5_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g021208mg PE=4 SV=1)

HSP 1 Score: 165.2 bits (417), Expect = 1.0e-37
Identity = 112/272 (41.18%), Postives = 152/272 (55.88%), Query Frame = 1


Query: 12  NYPHDYTLDELNFPTNFPATEKLITSVQLDSISSSQFISFGDSPEIEKLDCAMGKVERGK 71
           N  H YT    N  T     +K + +   +S  +    +   S  + ++     K E G 
Sbjct: 44  NLQHSYTHPSFNNNTGIDRPQKQLKTSTWNSSETGFNPNNVSSINMNQMGILKPKQETGA 103

Query: 72  DL-SFIIPQ--SLGVNQNRYVIGYEEGHDIKKRATIGRNS--GNGQQHVIAERRRREKLS 131
              S I+P   S G NQN YV    +G    KR + G  +   N Q H+IAER+RREKLS
Sbjct: 104 GYPSDILPSQVSFGNNQN-YVFKASQG---AKRISPGAGNRLSNTQDHIIAERKRREKLS 163

Query: 132 QSFAALSALIPGLNKTDKASVLGGAIKYVKELQERVKWAEEEA----------------- 191
           Q F ALSA++PGL K DKASVLG AIKY+K+LQE+VK  EE+A                 
Sbjct: 164 QRFIALSAIVPGLKKMDKASVLGDAIKYLKQLQEKVKILEEQANKKTIESVVFVNKTQLS 223

Query: 192 --GDE-EGRVIKSVPKIETRVLERDVLVKIHCKKQKGCFSNILTQIQKLKLTIVNSCVLP 251
             GD   G   +++P+IE R  ++ VL+++HC+K+KG F  I+ +I+KL LT++NS V+ 
Sbjct: 224 DEGDNPNGAFNEALPEIEARFCDKSVLIRVHCEKRKGVFEKIVAEIEKLHLTVINSSVMT 283

Query: 252 FGHSRLDITIIAQMEVGFCMTATDLGNKLTQA 259
           FG S LD+TIIAQM+V F MT  DL   L  A
Sbjct: 284 FGSSVLDVTIIAQMDVEFNMTVKDLVKNLHSA 311

[Close]

BLAST of CmaCh03G008670.1 vs. TrEMBL
Match: A0A0A0KG27_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G497110 PE=4 SV=1)

HSP 1 Score: 164.9 bits (416), Expect = 1.4e-37
Identity = 114/264 (43.18%), Postives = 141/264 (53.41%), Query Frame = 1


Query: 42  SISSSQFISFGD---SPEIEKLDCAMGKVERGKDLSFIIPQSLGVNQNRYVIGY------ 101
           S SSSQ ISFG+   SP + +                  P     N N     Y      
Sbjct: 84  SSSSSQIISFGNIEMSPMVAQ------------------PSYDNNNNNNKTSNYYCSPNK 143

Query: 102 EEGHDIKKRATIGRNSGN-----GQQHVIAERRRREKLSQSFAALSALIPGLNKTDKASV 161
             G  IK+ A    NS N      Q HV+AER+RREKLSQ F ALSALIP L K DKAS+
Sbjct: 144 NHGVGIKRSAAAAMNSNNRSPLVAQDHVLAERKRREKLSQRFVALSALIPDLKKMDKASI 203

Query: 162 LGGAIKYVKELQERVKWAEEEA-------------GDEEGRVIKS--------------- 221
           LG AI Y+K+LQER+K A E+A              D+   +I S               
Sbjct: 204 LGDAITYIKDLQERLKVANEQAAKATVESVVFVNKSDDASTIIASDDSSEENSSSSSDGA 263

Query: 222 VPKIETRVLERDVLVKIHCKKQKGCFSNILTQIQKLKLTIVNSCVLPFGHSRLDITIIAQ 264
           +P +E RV  +DVL++IH KK KGC SNIL QI+KL LT++NS  LPFG+ RLDITIIAQ
Sbjct: 264 IPDVEARVSGKDVLLRIHGKKCKGCLSNILNQIEKLNLTVLNSSALPFGNFRLDITIIAQ 323

[Close]

BLAST of CmaCh03G008670.1 vs. TAIR10
Match: AT4G37850.1 (AT4G37850.1 basic helix-loop-helix (bHLH) DNA-binding superfamily protein)

HSP 1 Score: 137.5 bits (345), Expect = 1.2e-32
Identity = 86/192 (44.79%), Postives = 111/192 (57.81%), Query Frame = 1


Query: 99  KKRATIGRNSGNGQQHVIAERRRREKLSQSFAALSALIPGLNKTDKASVLGGAIKYVKEL 158
           K+     RN  N Q H+IAER+RREKL+Q F ALSAL+PGL K DKASVLG A+K++K L
Sbjct: 138 KRAQPFSRNQSNAQDHIIAERKRREKLTQRFVALSALVPGLKKMDKASVLGDALKHIKYL 197

Query: 159 QERVKWAEEEAGD---------------------------EEGRVIKSVPKIETRVLERD 218
           QERV   EE+  +                           E+G     +P+IE R  + D
Sbjct: 198 QERVGELEEQKKERRLESMVLVKKSKLILDDNNQSFSSSCEDGFSDLDLPEIEVRFSDED 257

Query: 219 VLVKIHCKKQKGCFSNILTQIQKLKLTIVNSCVLPFGHSRLDITIIAQMEVGFCMTATDL 264
           VL+KI C+KQKG  + I+ +I+KL + I NS VL FG + LDITIIA+ E  F MT  D+
Sbjct: 258 VLIKILCEKQKGHLAKIMAEIEKLHILITNSSVLNFGPT-LDITIIAKKESDFDMTLMDV 317

[Close]

BLAST of CmaCh03G008670.1 vs. TAIR10
Match: AT2G22750.2 (AT2G22750.2 basic helix-loop-helix (bHLH) DNA-binding superfamily protein)

HSP 1 Score: 127.5 bits (319), Expect = 1.2e-29
Identity = 84/194 (43.30%), Postives = 110/194 (56.70%), Query Frame = 1


Query: 99  KKRATIGRNSGNGQQHVIAERRRREKLSQSFAALSALIPGLNKTDKASVLGGAIKYVKEL 158
           K+  ++ R+  N Q H++AER+RREKL+Q F ALSALIPGL K DKASVLG AIK++K L
Sbjct: 112 KRAQSLTRSQSNAQDHILAERKRREKLTQRFVALSALIPGLKKMDKASVLGDAIKHIKYL 171

Query: 159 QERVKWAEEEAGDEEGRVIKSV------------------------------PKIETRVL 218
           QE VK  EE+  ++    +  V                              P+IE RV 
Sbjct: 172 QESVKEYEEQKKEKTMESVVLVKKSSLVLDENHQPSSSSSSDGNRNSSSSNLPEIEVRVS 231

Query: 219 ERDVLVKIHCKKQKGCFSNILTQIQKLKLTIVNSCVLPFGHSRLDITIIAQMEVGFCMTA 263
            +DVL+KI C+KQKG    I+ +I+KL L+I NS VLPFG +  DI+IIAQ    F M  
Sbjct: 232 GKDVLIKILCEKQKGNVIKIMGEIEKLGLSITNSNVLPFGPT-FDISIIAQKNNNFDMKI 291

[Close]

BLAST of CmaCh03G008670.1 vs. TAIR10
Match: AT2G22760.1 (AT2G22760.1 basic helix-loop-helix (bHLH) DNA-binding superfamily protein)

HSP 1 Score: 124.4 bits (311), Expect = 1.0e-28
Identity = 74/178 (41.57%), Postives = 107/178 (60.11%), Query Frame = 1


Query: 112 QQHVIAERRRREKLSQSFAALSALIPGLNKTDKASVLGGAIKYVKELQERVKWAEEEAG- 171
           ++HV+AER+RREKLS+ F ALSAL+PGL K DK ++L  AI  +K+LQE+++  +EE   
Sbjct: 118 KEHVLAERKRREKLSEKFIALSALLPGLKKADKVTILDDAISRMKQLQEQLRTLKEEKEA 177

Query: 172 -----------------DEEGRVI----------KSVPKIETRVLERDVLVKIHCKKQKG 231
                            DEE  +           +++P+IE ++ + D+L++I C+K KG
Sbjct: 178 TRQMESMILVKKSKVFFDEEPNLSCSPSVHIEFDQALPEIEAKISQNDILIRILCEKSKG 237

Query: 232 CFSNILTQIQKLKLTIVNSCVLPFGHSRLDITIIAQMEVGFCMT-ATDLGNKLTQALV 261
           C  NIL  I+  +L I NS VLPFG S LDIT++AQM+  F M+   DL   L  A+V
Sbjct: 238 CMINILNTIENFQLRIENSIVLPFGDSTLDITVLAQMDKDFSMSILKDLVRNLRLAMV 295

[Close]

BLAST of CmaCh03G008670.1 vs. TAIR10
Match: AT2G22770.1 (AT2G22770.1 basic helix-loop-helix (bHLH) DNA-binding superfamily protein)

HSP 1 Score: 77.0 bits (188), Expect = 1.9e-14
Identity = 36/93 (38.71%), Postives = 59/93 (63.44%), Query Frame = 1


Query: 169 AGDEEGRVIKSVPKIETRVLERDVLVKIHCKKQKGCFSNILTQIQKLKLTIVNSCVLPFG 228
           + DE     +++P IE RV +RD+L+++HC+K KGC   IL+ ++K +L +VNS  LPFG
Sbjct: 228 SSDEVSIFKQTMPMIEARVSDRDLLIRVHCEKNKGCMIKILSSLEKFRLEVVNSFTLPFG 287

Query: 229 HSRLDITIIAQMEVGFCMTATDLGNKLTQALVD 262
           +S L ITI+ +M+  F     ++   +  AL +
Sbjct: 288 NSTLVITILTKMDNKFSRPVEEVVKNIRVALAE 320

[Close]

BLAST of CmaCh03G008670.1 vs. TAIR10
Match: AT2G46510.1 (AT2G46510.1 ABA-inducible BHLH-type transcription factor)

HSP 1 Score: 75.9 bits (185), Expect = 4.2e-14
Identity = 43/111 (38.74%), Postives = 69/111 (62.16%), Query Frame = 1


Query: 97  DIKKRATIGRNSGNGQQ----HVIAERRRREKLSQSFAALSALIPGLNKTDKASVLGGAI 156
           D K+    GR   NG++    HV AER+RREKL+Q F AL +++P ++K DKAS+LG AI
Sbjct: 375 DEKRPRKRGRKPANGREEPLNHVEAERQRREKLNQRFYALRSVVPNISKMDKASLLGDAI 434

Query: 157 KYVKELQERVKWAEEE-------AGDEEGRVIKSVPKIETRVLERDVLVKI 197
            Y+KELQE+VK  E+E         +     ++  P+++ + +  +V+V++
Sbjct: 435 SYIKELQEKVKIMEDERVGTDKSLSESNTITVEESPEVDIQAMNEEVVVRV 485

[Close]

BLAST of CmaCh03G008670.1 vs. NCBI nr
Match: gi|449451571|ref|XP_004143535.1| (PREDICTED: transcription factor bHLH25-like [Cucumis sativus])

HSP 1 Score: 245.4 bits (625), Expect = 1.1e-61
Identity = 156/304 (51.32%), Postives = 191/304 (62.83%), Query Frame = 1


Query: 2   MDCAYQYE-ITNYPHDYTLDELNFPTNFPATEKLITSVQLDSISSSQFISFGDSPEIEKL 61
           M+C+Y+YE +  YPHDYTLDELNFP    A EK+      +S SS Q ISFGD       
Sbjct: 1   MECSYEYERMKKYPHDYTLDELNFP----AMEKVN-----NSRSSGQLISFGDYS----- 60

Query: 62  DCAMGKVERGKDLSFIIPQSLGVNQNRYVIGYEEG-HDIKKR--ATI-------GRNSGN 121
               G++E  K            +    +IG+EE  H+IKK   +TI         ++ N
Sbjct: 61  ----GEIENYK-----------FDDYGNLIGFEEEEHEIKKDYCSTIIMGQLPNHNHNNN 120

Query: 122 GQQHVIAERRRREKLSQSFAALSALIPGLNKTDKASVLGGAIKYVKELQERVKWAEEEAG 181
             +HVIAERRRREK+ Q+F ALSALIPGL K DKASVLGGAIK+VKELQER+KWAEE+  
Sbjct: 121 NSEHVIAERRRREKIRQNFIALSALIPGLIKRDKASVLGGAIKFVKELQERLKWAEEKE- 180

Query: 182 DEEGRVIK-------------------------------SVPKIETRVLERDVLVKIHCK 241
            E+ RVIK                               SVP IETRVLE+DVLV+IHCK
Sbjct: 181 KEQKRVIKSVVFVKTINLDSDFDNETFSLDENGGRFSVRSVPTIETRVLEKDVLVRIHCK 240

Query: 242 KQKGCFSNILTQIQKLKLTIVNSCVLPFGHSRLDITIIAQMEVGFCMTATDLGNKLTQAL 264
           K KGC+++I+++I+KLKLTIVNSCV PFG SRLDITIIA+ME GFCMT  DLG KL + L
Sbjct: 241 KHKGCYTSIVSEIEKLKLTIVNSCVFPFGQSRLDITIIAEMEAGFCMTPMDLGKKLRETL 274

[Close]

BLAST of CmaCh03G008670.1 vs. NCBI nr
Match: gi|659079833|ref|XP_008440468.1| (PREDICTED: transcription factor bHLH25-like [Cucumis melo])

HSP 1 Score: 239.2 bits (609), Expect = 8.2e-60
Identity = 155/307 (50.49%), Postives = 191/307 (62.21%), Query Frame = 1


Query: 1   MMDCAYQYE-ITNYPHDYTLDELNFPTNFPATEKLITSVQLDSISSSQFISFGD-SPEIE 60
           MM+C+Y+YE +   PHDYTLDELNFP    A EK + +++    SSSQ +SFGD S EIE
Sbjct: 14  MMECSYEYERMKKCPHDYTLDELNFP----AKEK-VNNLR----SSSQLVSFGDYSGEIE 73

Query: 61  --KLDCAMGKVERGKDLSFIIPQSLGVNQNRYVIGYEEGHDIKKR--ATI------GRNS 120
             K D                      N    +   E+GH+IKK   +TI       RN 
Sbjct: 74  NYKFD----------------------NYGNLIGSGEKGHEIKKDYCSTIIMGQLPNRNR 133

Query: 121 G-NGQQHVIAERRRREKLSQSFAALSALIPGLNKTDKASVLGGAIKYVKELQERVKWAEE 180
             N  +HVIAERRRREK+ Q+F ALSALIPGL K DKASVLGGAI+YVKELQER+KWAEE
Sbjct: 134 NHNNSEHVIAERRRREKIRQNFIALSALIPGLIKRDKASVLGGAIEYVKELQERLKWAEE 193

Query: 181 EAG-----------------------------DEEG--RVIKSVPKIETRVLERDVLVKI 240
           EA                              DE G    ++SVP IE RVLE+DV+V+I
Sbjct: 194 EAEKQKSVIKSVVFVKRINLDSDDSENETFSLDENGDRSSVRSVPTIEIRVLEKDVMVRI 253

Query: 241 HCKKQKGCFSNILTQIQKLKLTIVNSCVLPFGHSRLDITIIAQMEVGFCMTATDLGNKLT 264
           HCKK++GC+++IL+QI+KLKLTI+NSCV PFG SRLDITI+A+ME GFCMT  D+G  L 
Sbjct: 254 HCKKRQGCYTSILSQIEKLKLTILNSCVSPFGESRLDITIVAEMEAGFCMTPMDVGKNLR 289

[Close]

BLAST of CmaCh03G008670.1 vs. NCBI nr
Match: gi|700193457|gb|KGN48661.1| (hypothetical protein Csa_6G497120 [Cucumis sativus])

HSP 1 Score: 236.5 bits (602), Expect = 5.3e-59
Identity = 157/323 (48.61%), Postives = 192/323 (59.44%), Query Frame = 1


Query: 1   MMDCAYQYE-ITNYPHDYTLDELNFPTNFPATEKLITSVQLDSISSSQFISFGDSPEIEK 60
           MM+C+Y+YE +  YPHDYTLDELNFP    A EK+      +S SS Q ISFGD      
Sbjct: 1   MMECSYEYERMKKYPHDYTLDELNFP----AMEKVN-----NSRSSGQLISFGDYS---- 60

Query: 61  LDCAMGKVERGKDLSFIIPQSLGVNQNRYVIGYEEG-HDIKKR--ATI-------GRNSG 120
                G++E  K            +    +IG+EE  H+IKK   +TI         ++ 
Sbjct: 61  -----GEIENYK-----------FDDYGNLIGFEEEEHEIKKDYCSTIIMGQLPNHNHNN 120

Query: 121 NGQQHVIAERRRREKLSQSFAALSALIPGLNK------------------TDKASVLGGA 180
           N  +HVIAERRRREK+ Q+F ALSALIPGL K                   DKASVLGGA
Sbjct: 121 NNSEHVIAERRRREKIRQNFIALSALIPGLIKDHDGHTVLQVEVSVEGLKADKASVLGGA 180

Query: 181 IKYVKELQERVKWAEEEAGDEEGRVIK-------------------------------SV 240
           IK+VKELQER+KWAEE+   E+ RVIK                               SV
Sbjct: 181 IKFVKELQERLKWAEEKE-KEQKRVIKSVVFVKTINLDSDFDNETFSLDENGGRFSVRSV 240

Query: 241 PKIETRVLERDVLVKIHCKKQKGCFSNILTQIQKLKLTIVNSCVLPFGHSRLDITIIAQM 264
           P IETRVLE+DVLV+IHCKK KGC+++I+++I+KLKLTIVNSCV PFG SRLDITIIA+M
Sbjct: 241 PTIETRVLEKDVLVRIHCKKHKGCYTSIVSEIEKLKLTIVNSCVFPFGQSRLDITIIAEM 293

[Close]

BLAST of CmaCh03G008670.1 vs. NCBI nr
Match: gi|659079829|ref|XP_008440466.1| (PREDICTED: transcription factor bHLH18-like [Cucumis melo])

HSP 1 Score: 182.6 bits (462), Expect = 9.1e-43
Identity = 122/293 (41.64%), Postives = 163/293 (55.63%), Query Frame = 1


Query: 14  PHDYTLDELNFPT-------NFPATEKLITSVQLDSISSSQFISFGDSPEIEKLDCA-MG 73
           P +++LD L F          +   EK    V+++     Q  + G    I K     +G
Sbjct: 28  PFEFSLDALQFDDLYELPVPPYAVEEKPAKQVKVEKEEEEQAGAGG----INKCKTTFVG 87

Query: 74  KVERGKDLSFIIPQ---SLGVNQNRYVIGYEEGHDIKKRATIGRNSGNGQQHVIAERRRR 133
              +G+D S        S   N N   +     ++ K+  ++ RN+ NG++HVIAER+RR
Sbjct: 88  NNYKGRDSSLSSSSRIISFENNWNHESVNNWNSNNGKRSCSMNRNAMNGREHVIAERKRR 147

Query: 134 EKLSQSFAALSALIPGLNKTDKASVLGGAIKYVKELQERVKWAEEE-------------- 193
           EKLSQ F ALSALIPGLNK DKAS+LGGAI++VKELQER+K  EE+              
Sbjct: 148 EKLSQRFIALSALIPGLNKVDKASILGGAIRHVKELQERLKGVEEQTTSKTSEPQVPVVC 207

Query: 194 ----------------AGDEE---GRVIKSVPKIETRVLERDVLVKIHCKKQKGCFSNIL 253
                           + DE    GR ++S P+IE R L  DVL++IHC K+KGC S +L
Sbjct: 208 VKRTTLQPSSSDDDTSSSDENSFSGR-LRSTPEIEARFLNNDVLIRIHCHKRKGCLSYLL 267

Query: 254 TQIQKL-KLTIVNSCVLPFGHSRLDITIIAQMEVGFCMTATDLGNKLTQALVD 262
            +IQ L  LTI+N+C LPF HS LDITI+AQM+VGF MT  D+   L QAL+D
Sbjct: 268 NRIQSLNNLTILNTCALPFSHSNLDITIVAQMDVGFHMTVEDVVKNLRQALLD 315

[Close]

BLAST of CmaCh03G008670.1 vs. NCBI nr
Match: gi|657946145|ref|XP_008383359.1| (PREDICTED: transcription factor bHLH18-like [Malus domestica])

HSP 1 Score: 179.9 bits (455), Expect = 5.9e-42
Identity = 114/270 (42.22%), Postives = 156/270 (57.78%), Query Frame = 1


Query: 35  ITSVQLDSISSSQFISFGDSP--------EIEKLDCAMGKVER------GK-DLSFIIPQ 94
           I + +    SSS  ISF +S         +   LDC + K +       GK +L  +I Q
Sbjct: 94  IIAAKASXSSSSHLISFENSDSSPPTTSQQYNGLDCKVIKPKNEVEYSNGKLNLPALISQ 153

Query: 95  SLGVNQNRYVIGYEEGHDIKKRATIGRNSGNGQQHVIAERRRREKLSQSFAALSALIPGL 154
               + +      + G  IK+ AT+ R+  + Q HV+AER+RREKLSQ F ALSAL+PGL
Sbjct: 154 G---SYDTQTCSPKHGXGIKRAATVTRSPLHAQDHVLAERKRREKLSQRFIALSALLPGL 213

Query: 155 NKTDKASVLGGAIKYVKELQERVKWAEEEAGDEEGRVI---------------------- 214
            K DKASVLG AIKYVK LQER K  EE+A  + G  +                      
Sbjct: 214 KKMDKASVLGDAIKYVKHLQERTKVLEEQAAKKTGEAVVFVKRMQYSADDDISSSDENSE 273

Query: 215 ----KSVPKIETRVLERDVLVKIHCKKQKGCFSNILTQIQKLKLTIVNSCVLPFGHSRLD 264
               + +P+IE RV +++VL++IHC+K KGC ++IL++I+KL LTIV+SC LPFG+S LD
Sbjct: 274 SCSDQPLPEIEARVSDKEVLIRIHCEKTKGCLTSILSEIEKLGLTIVHSCALPFGNSTLD 333

The following BLAST results are available for this feature:

BLAST of CmaCh03G008670.1 vs. Swiss-Prot


Match Name	E-value	Identity	Description
BH025_ARATH	2.1e-31	44.79	Transcription factor bHLH25 OS=Arabidopsis thaliana GN=BHLH25 PE=2 SV=2	[more]
BH018_ARATH	2.2e-28	43.30	Transcription factor bHLH18 OS=Arabidopsis thaliana GN=BHLH18 PE=2 SV=1	[more]
BH019_ARATH	1.8e-27	41.57	Transcription factor bHLH19 OS=Arabidopsis thaliana GN=BHLH19 PE=2 SV=1	[more]
BH020_ARATH	3.4e-13	38.71	Transcription factor NAI1 OS=Arabidopsis thaliana GN=NAI1 PE=2 SV=1	[more]
AIB_ARATH	7.5e-13	38.74	Transcription factor ABA-INDUCIBLE bHLH-TYPE OS=Arabidopsis thaliana GN=AIB PE=2...	[more]

BLAST of CmaCh03G008670.1 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KLM8_CUCSA	3.7e-59	48.61	Uncharacterized protein OS=Cucumis sativus GN=Csa_6G497120 PE=4 SV=1	[more]
M5WL55_PRUPE	1.1e-39	50.52	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa017640mg PE=4 SV=1	[more]
A0A0B0PCU7_GOSAR	8.0e-38	38.67	Transcription factor bHLH19-like protein OS=Gossypium arboreum GN=F383_28381 PE=...	[more]
A0A067FXL5_CITSI	1.0e-37	41.18	Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g021208mg PE=4 SV=1	[more]
A0A0A0KG27_CUCSA	1.4e-37	43.18	Uncharacterized protein OS=Cucumis sativus GN=Csa_6G497110 PE=4 SV=1	[more]

BLAST of CmaCh03G008670.1 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G37850.1	1.2e-32	44.79	basic helix-loop-helix (bHLH) DNA-binding superfamily protein	[more]
AT2G22750.2	1.2e-29	43.30	basic helix-loop-helix (bHLH) DNA-binding superfamily protein	[more]
AT2G22760.1	1.0e-28	41.57	basic helix-loop-helix (bHLH) DNA-binding superfamily protein	[more]
AT2G22770.1	1.9e-14	38.71	basic helix-loop-helix (bHLH) DNA-binding superfamily protein	[more]
AT2G46510.1	4.2e-14	38.74	ABA-inducible BHLH-type transcription factor	[more]

BLAST of CmaCh03G008670.1 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449451571\|ref\|XP_004143535.1\|	1.1e-61	51.32	PREDICTED: transcription factor bHLH25-like [Cucumis sativus]	[more]
gi\|659079833\|ref\|XP_008440468.1\|	8.2e-60	50.49	PREDICTED: transcription factor bHLH25-like [Cucumis melo]	[more]
gi\|700193457\|gb\|KGN48661.1\|	5.3e-59	48.61	hypothetical protein Csa_6G497120 [Cucumis sativus]	[more]
gi\|659079829\|ref\|XP_008440466.1\|	9.1e-43	41.64	PREDICTED: transcription factor bHLH18-like [Cucumis melo]	[more]
gi\|657946145\|ref\|XP_008383359.1\|	5.9e-42	42.22	PREDICTED: transcription factor bHLH18-like [Malus domestica]	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **INTERPRO**
Term	Definition
IPR011598	bHLH_dom

Vocabulary: **Molecular Function**
Term	Definition
GO:0046983	protein dimerization activity

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0046983	protein dimerization activity

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CmaCh03G008670	CmaCh03G008670	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CmaCh03G008670.1	CmaCh03G008670.1-protein	polypeptide

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmaCh03G008670.1.exon.1	CmaCh03G008670.1.exon.1	exon
CmaCh03G008670.1.exon.2	CmaCh03G008670.1.exon.2	exon
CmaCh03G008670.1.exon.3	CmaCh03G008670.1.exon.3	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmaCh03G008670.1.CDS.1	CmaCh03G008670.1.CDS.1	CDS
CmaCh03G008670.1.CDS.2	CmaCh03G008670.1.CDS.2	CDS
CmaCh03G008670.1.CDS.3	CmaCh03G008670.1.CDS.3	CDS

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain	GENE3D	G3DSA:4.10.280.10		coord: 113..169 score: 1.9
IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain	PFAM	PF00010	HLH	coord: 113..159 score: 2.9
IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain	SMART	SM00353	finulus	coord: 115..164 score: 1.1
IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain	PROFILE	PS50888	BHLH	coord: 109..158 score: 15
IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain	unknown	SSF47459	HLH, helix-loop-helix DNA-binding domain	coord: 108..170 score: 4.45
None	No IPR available	unknown	Coil	Coil	coord: 155..175 scor
None	No IPR available	PANTHER	PTHR23042	CIRCADIAN PROTEIN CLOCK/ARNT/BMAL/PAS	coord: 1..262 score: 1.1
None	No IPR available	PANTHER	PTHR23042:SF61	TRANSCRIPTION FACTOR BHLH18-RELATED	coord: 1..262 score: 1.1