CmaCh02G007460.1 (mRNA) Cucurbita maxima (Rimu)

BLAST of CmaCh02G007460.1 vs. Swiss-Prot
Match: NPC1_ARATH (Non-specific phospholipase C1 OS=Arabidopsis thaliana GN=NPC1 PE=2 SV=1)

HSP 1 Score: 844.0 bits (2179), Expect = 1.0e-243
Identity = 407/523 (77.82%), Postives = 458/523 (87.57%), Query Frame = 1

Query: 55  MVLRRAILTSFFLIYLLFSSQALEFDFKKRRHEIEGPIKTVVVLVMENRSFDHVLGWLKS 114
           M  RR + T     YLL SSQ++EF   ++ H+I+GPIKT+VV+VMENRSFDH+LGWLKS
Sbjct: 1   MAFRRVLTTVILFCYLLISSQSIEFKNSQKPHKIQGPIKTIVVVVMENRSFDHILGWLKS 60

Query: 115 VRPEIDGLTGKESNRVSVSDPNSEEIFVSSDAVFIDSDPGHSFQAISEQIFGSNDSSANP 174
            RPEIDGLTGKESN ++VSDPNS++IFVS DAVF+D DPGHSFQAI EQIFGSND+S +P
Sbjct: 61  TRPEIDGLTGKESNPLNVSDPNSKKIFVSDDAVFVDMDPGHSFQAIREQIFGSNDTSGDP 120

Query: 175 APMNGFAQQAASMEAVDMPTTVMSGFKPELVPVYTELANQFAIFDRWFASVPASTQPNRF 234
             MNGFAQQ+ SME   M   VMSGFKPE++PVYTELAN+F +FDRWFASVP STQPNRF
Sbjct: 121 K-MNGFAQQSESMEP-GMAKNVMSGFKPEVLPVYTELANEFGVFDRWFASVPTSTQPNRF 180

Query: 235 YVHSATSHGAMSNVRKDLIHGFPQKTIFDSLDENGLSFGIYYQNIPATLFFKSLRRLKHI 294
           YVHSATSHG  SNV+KDL+ GFPQKTIFDSLDENGLSFGIYYQNIPAT FFKSLRRLKH+
Sbjct: 181 YVHSATSHGCSSNVKKDLVKGFPQKTIFDSLDENGLSFGIYYQNIPATFFFKSLRRLKHL 240

Query: 295 VKFHSYAPKFKLHAKLGRLPNYAVIEQRYFDVNLFPANDDHPSHDVARGQQFVKEVYEIL 354
           VKFHSYA KFKL AKLG+LPNY+V+EQRYFD++LFPANDDHPSHDVA GQ+FVKEVYE L
Sbjct: 241 VKFHSYALKFKLDAKLGKLPNYSVVEQRYFDIDLFPANDDHPSHDVAAGQRFVKEVYETL 300

Query: 355 RASPQWKEMALLITYDEHGGFYDHVPTPVSGVPSPDGIIGPDPYYFRFDRLGVRVPTILV 414
           R+SPQWKEMALLITYDEHGGFYDHVPTPV GVP+PDGIIGPDP+YF FDRLGVRVPT L+
Sbjct: 301 RSSPQWKEMALLITYDEHGGFYDHVPTPVKGVPNPDGIIGPDPFYFGFDRLGVRVPTFLI 360

Query: 415 SPWVEKGTVIREPVGPKSTSQFEHSSIPATVKNLFNLKSNFLTKRDAWAGTFEHYLKLRD 474
           SPW+EKGTVI EP GP   SQFEHSSIPATVK LFNLKS+FLTKRDAWAGTFE Y ++RD
Sbjct: 361 SPWIEKGTVIHEPEGPTPHSQFEHSSIPATVKKLFNLKSHFLTKRDAWAGTFEKYFRIRD 420

Query: 475 SPRNDCPETLPKVTAPLRPWGPKEHAKLSEFQVELIQLASQLNGDYVLNSYPNIGKYMTV 534
           SPR DCPE LP+V   LRPWG KE +KLSEFQVELIQLASQL GD++LNSYP+IGK MTV
Sbjct: 421 SPRQDCPEKLPEVKLSLRPWGAKEDSKLSEFQVELIQLASQLVGDHLLNSYPDIGKNMTV 480

Query: 535 GEANRYAEDAVKRFLEAGKVALMAGANESAIVTMRPSLTSRTA 578
            E N+YAEDAV++FLEAG  AL AGA+E+ IVTMRPSLT+RT+
Sbjct: 481 SEGNKYAEDAVQKFLEAGMAALEAGADENTIVTMRPSLTTRTS 521

BLAST of CmaCh02G007460.1 vs. Swiss-Prot
Match: NPC2_ARATH (Non-specific phospholipase C2 OS=Arabidopsis thaliana GN=NPC2 PE=2 SV=1)

HSP 1 Score: 635.2 bits (1637), Expect = 7.2e-181
Identity = 311/488 (63.73%), Postives = 382/488 (78.28%), Query Frame = 1

Query: 91  PIKTVVVLVMENRSFDHVLGWLKSVRPEIDGLTGKESNRVSVSDPNSEEIFVSSDAVFID 150
           PIKT+VV+VMENRSFDH+LGW+K + PEI+G+ G ESN VSVSDP+S +I   S + ++D
Sbjct: 26  PIKTIVVVVMENRSFDHMLGWMKKLNPEINGVDGSESNPVSVSDPSSRKIKFGSGSHYVD 85

Query: 151 SDPGHSFQAISEQIFGSNDSSANPAPMNGFAQQAASME-AVDMPTTVMSGFKPELVPVYT 210
            DPGHSFQAI EQ+FGSND+S +P PMNGF QQA S + + +M  +VM+GF+P+ VPVY 
Sbjct: 86  PDPGHSFQAIREQVFGSNDTSMDPPPMNGFVQQAYSEDPSGNMSASVMNGFEPDKVPVYK 145

Query: 211 ELANQFAIFDRWFASVPASTQPNRFYVHSATSHGAMSNVRKDLIHGFPQKTIFDSLDENG 270
            L ++FA+FDRWFASVP+STQPNR +VHS TS GA SN    L  G+PQ+TIFD+LD+  
Sbjct: 146 SLVSEFAVFDRWFASVPSSTQPNRMFVHSGTSAGATSNNPISLAKGYPQRTIFDNLDDEE 205

Query: 271 LSFGIYYQNIPATLFFKSLRRLKHIVKFHSYAPKFKLHAKLGRLPNYAVIEQRYFDVNLF 330
            SFGIYYQNIPA LF++SLR+LK++ KFHSY   FK HAK G+LP Y VIEQRY D  L 
Sbjct: 206 FSFGIYYQNIPAVLFYQSLRKLKYVFKFHSYGNSFKDHAKQGKLPAYTVIEQRYMDTLLE 265

Query: 331 PANDDHPSHDVARGQQFVKEVYEILRASPQWKEMALLITYDEHGGFYDHVPTPVSGVPSP 390
           PA+DDHPSHDV +GQ+F+KEVYE LRASPQW E  L+ITYDEHGG++DHVPTPV  VPSP
Sbjct: 266 PASDDHPSHDVYQGQKFIKEVYETLRASPQWNETLLIITYDEHGGYFDHVPTPVRNVPSP 325

Query: 391 DGIIGPDPYYFRFDRLGVRVPTILVSPWVEKGTVIREPVG-PKSTSQFEHSSIPATVKNL 450
           DGI+GPDP+ F+F+RLG+RVPTI VSPW+EKGTV+  P G P  +S++EHSSIPATVK L
Sbjct: 326 DGIVGPDPFLFQFNRLGIRVPTIAVSPWIEKGTVVHGPNGSPFPSSEYEHSSIPATVKKL 385

Query: 451 FNLKSNFLTKRDAWAGTFEHYLKLRDSPRNDCPETLPKVTAPLRPWGPKEHAKLSEFQVE 510
           FNL S FLTKRD WAGTFE+ L++R  PR DCPETLP+    +R     E A L+EFQ E
Sbjct: 386 FNLSSPFLTKRDEWAGTFENILQIRKEPRTDCPETLPE-PVKIRMGEANEKALLTEFQQE 445

Query: 511 LIQLASQLNGDYVLNSYP-NIGKYMTVGEANRYAEDAVKRFLEAGKVALMAGANESAIVT 570
           L+QLA+ L GD +L ++P  I K MTV E  RY EDA+KRFLEAG++AL  GAN+  +V 
Sbjct: 446 LVQLAAVLKGDNMLTTFPKEISKGMTVIEGKRYMEDAMKRFLEAGRMALSMGANKEELVH 505

Query: 571 MRPSLTSR 576
           M+ SLT R
Sbjct: 506 MKTSLTGR 512

BLAST of CmaCh02G007460.1 vs. Swiss-Prot
Match: NPC6_ARATH (Non-specific phospholipase C6 OS=Arabidopsis thaliana GN=NPC6 PE=2 SV=1)

HSP 1 Score: 599.7 bits (1545), Expect = 3.3e-170
Identity = 298/493 (60.45%), Postives = 370/493 (75.05%), Query Frame = 1

Query: 86  HEIEGPIKTVVVLVMENRSFDHVLGWLK-SVRPEIDGLTGKESNRVSVSDPNSEE-IFVS 145
           H+ + PIKTVVVLV+ENRSFDH+LGW+K SV P I+G+TG+E N V    PNS + I  +
Sbjct: 33  HQWQSPIKTVVVLVLENRSFDHLLGWMKNSVNPTINGVTGQECNPV----PNSTQTICFT 92

Query: 146 SDAVFIDSDPGHSFQAISEQIFGSNDSSANPAPMNGFAQQAASMEAVDMPTTVMSGFKPE 205
           SDA F+D DPGHSF+A+ +Q+FGS         M GF +QA SM   ++  TVM GF+PE
Sbjct: 93  SDAEFVDPDPGHSFEAVEQQVFGSGPGQI--PSMMGFVEQALSMPG-NLSETVMKGFRPE 152

Query: 206 LVPVYTELANQFAIFDRWFASVPASTQPNRFYVHSATSHGAMSNVRKDLIHGFPQKTIFD 265
            VPVY EL  +FA+FDRWF+S+P  TQPNR +V+SATSHG+ S+V+K L  G+PQKTIFD
Sbjct: 153 AVPVYAELVKEFAVFDRWFSSIPGPTQPNRLFVYSATSHGSTSHVKKQLAQGYPQKTIFD 212

Query: 266 SLDENGLSFGIYYQNIPATLFFKSLRRLKHIVKFHSYAPKFKLHAKLGRLPNYAVIEQRY 325
           SL  N + FGIY+QNIP TLF+++LR+LK+I   H Y  KFK  A  G+LP+  VIE RY
Sbjct: 213 SLHSNDIDFGIYFQNIPTTLFYRNLRQLKYIFNLHQYDLKFKKDAAKGKLPSLTVIEPRY 272

Query: 326 FDVNLFPANDDHPSHDVARGQQFVKEVYEILRASPQWKEMALLITYDEHGGFYDHVPTPV 385
           FD+   PANDDHPSHDVA GQ+ VKEVYE LR+SPQW E  L+ITYDEHGGFYDHV TP 
Sbjct: 273 FDLKGLPANDDHPSHDVANGQKLVKEVYEALRSSPQWNETLLVITYDEHGGFYDHVKTPY 332

Query: 386 SGVPSPDGIIGPDPYYFRFDRLGVRVPTILVSPWVEKGTVIREPVGPKSTSQFEHSSIPA 445
            G+P+PDG  GP P +F+FDRLGVRVPTI+VSPW++KGTV+ E  GP  +S++EHSSIPA
Sbjct: 333 VGIPNPDGNTGPAPGFFKFDRLGVRVPTIMVSPWIQKGTVVSEAKGPTESSEYEHSSIPA 392

Query: 446 TVKNLFNLKSNFLTKRDAWAGTFEHYLKLRDSPRNDCPETLPKVTAPLRPWGPKEHAKLS 505
           T+K LFNL SNFLT RDAWA TFE  +    +PR DCP TLP+V AP+R   PKE A LS
Sbjct: 393 TIKKLFNLSSNFLTHRDAWAATFEDVVSHLTTPRTDCPMTLPEV-APMRATEPKEDAALS 452

Query: 506 EFQVELIQLASQLNGDYVLNSYP-NIGKYMTVGEANRYAEDAVKRFLEAGKVALMAGANE 565
           EFQ E++QLA+ LNGD+ L+S+P  IGK MTV +A+ Y + A  RF+ A K A+  GA++
Sbjct: 453 EFQGEVVQLAAVLNGDHFLSSFPEEIGKKMTVKQAHEYVKGATSRFIRASKEAMKLGADK 512

Query: 566 SAIVTMRPSLTSR 576
           SAIV MR SLT+R
Sbjct: 513 SAIVDMRSSLTTR 517

BLAST of CmaCh02G007460.1 vs. Swiss-Prot
Match: NPC3_ARATH (Non-specific phospholipase C3 OS=Arabidopsis thaliana GN=NPC3 PE=2 SV=1)

HSP 1 Score: 551.6 bits (1420), Expect = 1.0e-155
Identity = 272/491 (55.40%), Postives = 347/491 (70.67%), Query Frame = 1

Query: 91  PIKTVVVLVMENRSFDHVLGWLKSVRPEIDGLTGKE--SNRVSVSDPNSEEIFVSSDAVF 150
           PIKT+VVLV ENRSFDH+LGW K + PEIDG++  E  SN +S SDPNS +IF   ++  
Sbjct: 15  PIKTIVVLVQENRSFDHMLGWFKELNPEIDGVSESEPRSNPLSTSDPNSAQIFFGKESQN 74

Query: 151 IDSDPGHSFQAISEQIFGSNDSSANPAP---MNGFAQQAASMEAVDMPTTVMSGFKPELV 210
           ID DPGHSFQAI EQ+FG   S  +P P   MNGF Q A ++        VM GF PE +
Sbjct: 75  IDPDPGHSFQAIYEQVFGKPFSDESPYPDPKMNGFVQNAEAITKGMSEKVVMQGFPPEKL 134

Query: 211 PVYTELANQFAIFDRWFASVPASTQPNRFYVHSATSHGAMSNVRKDLIHGFPQKTIFDSL 270
           PV+ EL  +FA+ DRWF+S+P+STQPNR YVH+ATS+GA SN    L+ GFPQ+T+F+SL
Sbjct: 135 PVFKELVQEFAVCDRWFSSLPSSTQPNRLYVHAATSNGAFSNDTNTLVRGFPQRTVFESL 194

Query: 271 DENGLSFGIYYQNIPATLFFKSLRRLKHIVKFHSYAPKFKLHAKLGRLPNYAVIEQRYFD 330
           +E+G +FGIYYQ+ P  LF++++R+LK++  FH Y   FK H K G+LPNY VIE RYF 
Sbjct: 195 EESGFTFGIYYQSFPNCLFYRNMRKLKYVDNFHQYHLSFKRHCKEGKLPNYVVIEPRYFK 254

Query: 331 VNLFPANDDHPSHDVARGQQFVKEVYEILRASPQWKEMALLITYDEHGGFYDHVPTPVSG 390
           +   PANDDHP +DV  GQ  VKE+YE LRASPQW E+  ++ YDEHGG+YDHVPTPV G
Sbjct: 255 ILSAPANDDHPKNDVVEGQNLVKEIYEALRASPQWNEILFVVVYDEHGGYYDHVPTPVIG 314

Query: 391 VPSPDGIIGPDPYYFRFDRLGVRVPTILVSPWVEKGTVIREPVGPKSTSQFEHSSIPATV 450
           VP+PDG++GP+PY F+FDRLGVRVP +L+SPW+E GTV+ EP GP+ TSQFEHSSIPAT+
Sbjct: 315 VPNPDGLVGPEPYNFKFDRLGVRVPALLISPWIEPGTVLHEPNGPEPTSQFEHSSIPATL 374

Query: 451 KNLFNLKSNFLTKRDAWAGTFEHYLKLRDSPRNDCPETLPKV--TAPLRPWGPKEHAKLS 510
           K +FNLKS FLTKRD WAGT +  +  R SPR DCP TLP++     +     +E   L+
Sbjct: 375 KKIFNLKS-FLTKRDEWAGTLDAVIN-RTSPRTDCPVTLPELPRARDIDIGTQEEDEDLT 434

Query: 511 EFQVELIQLASQLNGDYVLNSYP-NIGKYMTVGEANRYAEDAVKRFLEAGKVALMAGANE 570
           +FQ+ELIQ A+ L GD++ + YP  +   M V +A RY E+A  RF    K A   G +E
Sbjct: 435 DFQIELIQAAAVLKGDHIKDIYPFKLADKMKVLDAARYVEEAFTRFHGESKKAKEEGRDE 494

Query: 571 SAIVTMRPSLT 574
             IV +    T
Sbjct: 495 HEIVDLSKGST 503

BLAST of CmaCh02G007460.1 vs. Swiss-Prot
Match: NPC4_ARATH (Non-specific phospholipase C4 OS=Arabidopsis thaliana GN=NPC4 PE=1 SV=1)

HSP 1 Score: 550.1 bits (1416), Expect = 3.0e-155
Identity = 282/485 (58.14%), Postives = 343/485 (70.72%), Query Frame = 1

Query: 91  PIKTVVVLVMENRSFDHVLGWLKSVRPEIDGLTGKE--SNRVSVSDPNSEEIFVSSDAVF 150
           PIKT+VVLV ENRSFDH LGW K +  EIDG+T  +  SN VS SD NS  +     + +
Sbjct: 13  PIKTIVVLVQENRSFDHTLGWFKELNREIDGVTKSDPKSNTVSSSDTNSLRVVFGDQSQY 72

Query: 151 IDSDPGHSFQAISEQIFGSNDSSANPAP------MNGFAQQAASMEAVDMPTTVMSGFKP 210
           ++ DPGHS Q I EQ+FG    S  P P      M+GFAQ  A      M + VM+GFKP
Sbjct: 73  VNPDPGHSIQDIYEQVFGKPWDSGKPDPNPGHPNMSGFAQN-AERNKKGMSSAVMNGFKP 132

Query: 211 ELVPVYTELANQFAIFDRWFASVPASTQPNRFYVHSATSHGAMSNVRKDLIHGFPQKTIF 270
             +PVY EL   FAI DRWFASVPASTQPNR YVHSATSHGA SN +K L+ GFPQKTIF
Sbjct: 133 NALPVYKELVQNFAICDRWFASVPASTQPNRLYVHSATSHGATSNDKKLLLEGFPQKTIF 192

Query: 271 DSLDENGLSFGIYYQNIPATLFFKSLRRLKHIVKFHSYAPKFKLHAKLGRLPNYAVIEQR 330
           +SLDE G SFGIYYQ  P+TLF+++LR+LK++  FH Y  +FK   K G+LPNY V+EQR
Sbjct: 193 ESLDEAGFSFGIYYQFPPSTLFYRNLRKLKYLTHFHQYGIQFKKDCKEGKLPNYVVVEQR 252

Query: 331 YFDVNLFPANDDHPSHDVARGQQFVKEVYEILRASPQWKEMALLITYDEHGGFYDHVPTP 390
           +FD+   PANDDHPSHDV+ GQ+ VKEVYE LR+SPQW E+  +ITYDEHGGFYDHVPTP
Sbjct: 253 WFDLLSTPANDDHPSHDVSEGQKLVKEVYEALRSSPQWNEILFIITYDEHGGFYDHVPTP 312

Query: 391 VSGVPSPDGIIGPDPYYFRFDRLGVRVPTILVSPWVEKGTVIREPVGPKSTSQFEHSSIP 450
           V GVP+PDGI+GP PY F F+RLGVRVPT  +SPW+E GTVI  P GP   SQ+EHSSIP
Sbjct: 313 VDGVPNPDGILGPPPYNFEFNRLGVRVPTFFISPWIEPGTVIHGPNGPYPRSQYEHSSIP 372

Query: 451 ATVKNLFNLKSNFLTKRDAWAGTFEHYLKLRDSPRNDCPETLPKVTAPLRPWGPKEHAKL 510
           ATVK +F LK +FL+KRD+WAGTFE  +  RDSPR DCPETL      LR    KE+A+L
Sbjct: 373 ATVKTIFKLK-DFLSKRDSWAGTFESVI-TRDSPRQDCPETL-STPIKLRGTMAKENAQL 432

Query: 511 SEFQVELIQLASQLNGDYVLNS-YPNIGKYMTVGEANRYAEDAVKRFLEAGKVALMAGAN 567
           SEFQ +L+ +A+ L GDY        + K   V +A++Y  +A ++FLE  + A   G +
Sbjct: 433 SEFQEDLVIMAAGLKGDYKNEELIHKLCKETCVADASKYVTNAFEKFLEESRKARDRGCD 492

BLAST of CmaCh02G007460.1 vs. TrEMBL
Match: W9RP53_9ROSA (Phospholipase C 4 OS=Morus notabilis GN=L484_022015 PE=4 SV=1)

HSP 1 Score: 894.0 bits (2309), Expect = 9.5e-257
Identity = 435/533 (81.61%), Postives = 475/533 (89.12%), Query Frame = 1

Query: 55  MVLRRAILTSFFLIYLLFSSQALEFDFKKRRHEIEGPIKTVVVLVMENRSFDHVLGWLKS 114
           M  RR  L S   +YL+ SS +L     +R+H+I+GPIKTVVVLVMENRSFDHVLGWLKS
Sbjct: 1   MGFRRTSLASIIFLYLMISSHSLHA-LSRRKHKIQGPIKTVVVLVMENRSFDHVLGWLKS 60

Query: 115 VRPEIDGLTGKESNRVSVSDPNSEEIFVSSDAVFIDSDPGHSFQAISEQIFGSNDSSANP 174
            RPEIDGLTGKE NR++VSDP S  +FVS DA+F+DSDPGHSFQAI EQIFGSN  S NP
Sbjct: 61  TRPEIDGLTGKEWNRINVSDPKSPNVFVSDDAIFVDSDPGHSFQAIREQIFGSNHGSENP 120

Query: 175 APMNGFAQQAASMEAVDMPTTVMSGFKPELVPVYTELANQFAIFDRWFASVPASTQPNRF 234
           APMNGFAQQA SM    MP TVMSGFKP  VPVYTELAN+FA+FDRWFASVPASTQPNRF
Sbjct: 121 APMNGFAQQAVSM-GEGMPRTVMSGFKPGRVPVYTELANEFAVFDRWFASVPASTQPNRF 180

Query: 235 YVHSATSHGAMSNVRKDLIHGFPQKTIFDSLDENGLSFGIYYQNIPATLFFKSLRRLKHI 294
           YVHSATSHGA SNVRKDLIHGFPQKTIFDSLDENGL FGIYYQNIPATLFFKSLR+LKH 
Sbjct: 181 YVHSATSHGATSNVRKDLIHGFPQKTIFDSLDENGLDFGIYYQNIPATLFFKSLRKLKHA 240

Query: 295 VKFHSYAPKFKLHAKLGRLPNYAVIEQRYFDVNLFPANDDHPSHDVARGQQFVKEVYEIL 354
           +KFH+YA KFKLHA+LG+LPNY V+EQRYFDV+LFPANDDHPSHDVA GQ+FVK+VYEIL
Sbjct: 241 IKFHNYALKFKLHARLGKLPNYVVLEQRYFDVDLFPANDDHPSHDVALGQRFVKDVYEIL 300

Query: 355 RASPQWKEMALLITYDEHGGFYDHVPTPVSGVPSPDGIIGPDPYYFRFDRLGVRVPTILV 414
           RASPQWKEMALLITYDEHGGFYDHVPTPVSGVPSPDGI+GPDP+YFRFDRLGVRVPTILV
Sbjct: 301 RASPQWKEMALLITYDEHGGFYDHVPTPVSGVPSPDGIVGPDPFYFRFDRLGVRVPTILV 360

Query: 415 SPWVEKGTVIREPVGPKSTSQFEHSSIPATVKNLFNLKSNFLTKRDAWAGTFEHYLKLRD 474
           SPWV+KGTVI EPVGP   SQFEHSSIPATVK LFNLKSNFLTKRDAWAGTFE+Y KLRD
Sbjct: 361 SPWVDKGTVIHEPVGPTPHSQFEHSSIPATVKKLFNLKSNFLTKRDAWAGTFENYFKLRD 420

Query: 475 SPRNDCPETLPKVTAPLRPWGPKEHAKLSEFQVELIQLASQLNGDYVLNSYPNIGKYMTV 534
           SPR+DCPETLP+V   LRPWGPKE A+LSEFQVELIQLASQLNGDY+LN+YPNIGK MTV
Sbjct: 421 SPRDDCPETLPEVKLSLRPWGPKEDARLSEFQVELIQLASQLNGDYMLNTYPNIGKTMTV 480

Query: 535 GEANRYAEDAVKRFLEAGKVALMAGANESAIVTMRPSLTSRTADVHSGQFIET 588
           GEAN+YAEDAVKRFLEAG+ AL AGAN++AIVTMRPSLTSR +   +  ++ET
Sbjct: 481 GEANKYAEDAVKRFLEAGRAALKAGANDTAIVTMRPSLTSRISTGGTRNYLET 531

BLAST of CmaCh02G007460.1 vs. TrEMBL
Match: B9GII8_POPTR (Phospholipase family protein OS=Populus trichocarpa GN=POPTR_0001s25760g PE=4 SV=1)

HSP 1 Score: 885.2 bits (2286), Expect = 4.4e-254
Identity = 428/532 (80.45%), Postives = 476/532 (89.47%), Query Frame = 1

Query: 55  MVLRRAILTSFFLIYLLFSSQALEFDFKKRRHE--IEGPIKTVVVLVMENRSFDHVLGWL 114
           M  RR  LTS   +YL+ SSQ+L+FD   +RH+  I+GPIKT+VVLVMENRSFDHVLGWL
Sbjct: 1   MTSRRLSLTSLLFLYLVVSSQSLDFDDLHKRHKPKIKGPIKTLVVLVMENRSFDHVLGWL 60

Query: 115 KSVRPEIDGLTGKESNRVSVSDPNSEEIFVSSDAVFIDSDPGHSFQAISEQIFGSNDSSA 174
           KS RP+IDGL+G ESNR+S SD NS+E+FVS DAVFIDSDPGHSFQAI EQIFGSNDS A
Sbjct: 61  KSTRPDIDGLSGSESNRISASDRNSDEVFVSDDAVFIDSDPGHSFQAIREQIFGSNDSFA 120

Query: 175 NPAPMNGFAQQAASMEAVDMPTTVMSGFKPELVPVYTELANQFAIFDRWFASVPASTQPN 234
           +PAPMNGF QQA SM    M  TVMSGFKP L+PVYTELAN+FA+FDRWFASVPASTQPN
Sbjct: 121 DPAPMNGFVQQAKSMGET-MSKTVMSGFKPSLLPVYTELANEFAVFDRWFASVPASTQPN 180

Query: 235 RFYVHSATSHGAMSNVRKDLIHGFPQKTIFDSLDENGLSFGIYYQNIPATLFFKSLRRLK 294
           RFYVHSATSHGAMSNVRKDLIHGFPQ+TIFDSLD+NGLSFGIYYQNIPATLFFKSLR+LK
Sbjct: 181 RFYVHSATSHGAMSNVRKDLIHGFPQRTIFDSLDDNGLSFGIYYQNIPATLFFKSLRKLK 240

Query: 295 HIVKFHSYAPKFKLHAKLGRLPNYAVIEQRYFDVNLFPANDDHPSHDVARGQQFVKEVYE 354
           H++KFHSY  KFKLHAKLG+LPNY V+EQRYFDV LFPANDDHPSHDVARGQ+FVKEVYE
Sbjct: 241 HLLKFHSYELKFKLHAKLGKLPNYVVVEQRYFDVELFPANDDHPSHDVARGQRFVKEVYE 300

Query: 355 ILRASPQWKEMALLITYDEHGGFYDHVPTPVSGVPSPDGIIGPDPYYFRFDRLGVRVPTI 414
           ILR+SPQWKEMALLITYDEHGGFYDHVPTPV GVP+PDGIIGPDPYYF+FDRLG+RVPT 
Sbjct: 301 ILRSSPQWKEMALLITYDEHGGFYDHVPTPVRGVPNPDGIIGPDPYYFQFDRLGIRVPTF 360

Query: 415 LVSPWVEKGTVIREPVGPKSTSQFEHSSIPATVKNLFNLKSNFLTKRDAWAGTFEHYLKL 474
           L+SPW++KGTVI EP GP+  SQFEHSSIPATVK LFNL SNFLT+RDAWAG+FE+Y  L
Sbjct: 361 LISPWIDKGTVIHEPDGPRPDSQFEHSSIPATVKKLFNLNSNFLTRRDAWAGSFENYFYL 420

Query: 475 RDSPRNDCPETLPKVTAPLRPWGPKEHAKLSEFQVELIQLASQLNGDYVLNSYPNIGKYM 534
           RD+PR+DCPETLP VT  LRPWGPKE A LSEFQVE+IQLASQLNGD+VLN+YP+IGK M
Sbjct: 421 RDTPRDDCPETLPDVTTSLRPWGPKEDASLSEFQVEMIQLASQLNGDHVLNAYPDIGKSM 480

Query: 535 TVGEANRYAEDAVKRFLEAGKVALMAGANESAIVTMRPSLTSRTADVHSGQF 585
           TVGEANRYAEDAV+RFLEAG+ AL AGANESAIVTM+PSLTSR     +G++
Sbjct: 481 TVGEANRYAEDAVRRFLEAGRAALRAGANESAIVTMKPSLTSRVPAGDNGRY 531

BLAST of CmaCh02G007460.1 vs. TrEMBL
Match: B9HP48_POPTR (Phospholipase family protein OS=Populus trichocarpa GN=POPTR_0009s04970g PE=4 SV=2)

HSP 1 Score: 877.5 bits (2266), Expect = 9.2e-252
Identity = 423/523 (80.88%), Postives = 470/523 (89.87%), Query Frame = 1

Query: 55  MVLRRAILTSFFLIYLLFSSQALEFD--FKKRRHEIEGPIKTVVVLVMENRSFDHVLGWL 114
           M  RR  L   F +YL+ SS +L+FD   KK + +I+GPIKT+V+LVMENRSFDHVLGWL
Sbjct: 1   MTCRRLPLAFLFFLYLVISSHSLDFDDLHKKHKSKIKGPIKTLVILVMENRSFDHVLGWL 60

Query: 115 KSVRPEIDGLTGKESNRVSVSDPNSEEIFVSSDAVFIDSDPGHSFQAISEQIFGSNDSSA 174
           KS RP+IDGLTG ESNR+SVSDPN++EIFVS DAVFIDSDPGHSFQAI EQIFGSNDS A
Sbjct: 61  KSTRPDIDGLTGSESNRISVSDPNADEIFVSDDAVFIDSDPGHSFQAIREQIFGSNDSLA 120

Query: 175 NPAPMNGFAQQAASMEAVDMPTTVMSGFKPELVPVYTELANQFAIFDRWFASVPASTQPN 234
           +PAPM+GFAQQA SM    M   VMSGFKP  VPVYTELAN+FA+FDRWFASVPASTQPN
Sbjct: 121 DPAPMSGFAQQAKSMSET-MSKVVMSGFKPSRVPVYTELANEFAVFDRWFASVPASTQPN 180

Query: 235 RFYVHSATSHGAMSNVRKDLIHGFPQKTIFDSLDENGLSFGIYYQNIPATLFFKSLRRLK 294
           R YVHSATSHGAMSNVRKDLIHGFPQKTIFDSLDENGLSFG+YYQNIPATLF KSLR+LK
Sbjct: 181 RLYVHSATSHGAMSNVRKDLIHGFPQKTIFDSLDENGLSFGVYYQNIPATLFLKSLRKLK 240

Query: 295 HIVKFHSYAPKFKLHAKLGRLPNYAVIEQRYFDVNLFPANDDHPSHDVARGQQFVKEVYE 354
           H +KFHSY  KFKLHAKLG+LPNY V+EQRYFDV LFPANDDHPSHD+ARGQ+FVKEVYE
Sbjct: 241 HAMKFHSYQLKFKLHAKLGKLPNYVVVEQRYFDVELFPANDDHPSHDMARGQRFVKEVYE 300

Query: 355 ILRASPQWKEMALLITYDEHGGFYDHVPTPVSGVPSPDGIIGPDPYYFRFDRLGVRVPTI 414
            LR+SPQWKEMALLITYDEHGGFYDHVPTPV GVP+PDGI+G DPYYF+F+RLGVRVPT+
Sbjct: 301 TLRSSPQWKEMALLITYDEHGGFYDHVPTPVRGVPNPDGIVGRDPYYFQFNRLGVRVPTL 360

Query: 415 LVSPWVEKGTVIREPVGPKSTSQFEHSSIPATVKNLFNLKSNFLTKRDAWAGTFEHYLKL 474
           L+SPW++KGTVI EP GP+ +SQFEHSSIPATVK LFNLKSNFLT+RDAWAG+FE+Y  L
Sbjct: 361 LISPWIDKGTVIHEPAGPRPSSQFEHSSIPATVKKLFNLKSNFLTRRDAWAGSFENYFYL 420

Query: 475 RDSPRNDCPETLPKVTAPLRPWGPKEHAKLSEFQVELIQLASQLNGDYVLNSYPNIGKYM 534
           RD+PR+DCPETLP+VT  +RPWGPKE A LSEFQVE+IQLASQLNGDYVLN+YP+IGK M
Sbjct: 421 RDTPRDDCPETLPEVTTLMRPWGPKEDASLSEFQVEMIQLASQLNGDYVLNAYPDIGKSM 480

Query: 535 TVGEANRYAEDAVKRFLEAGKVALMAGANESAIVTMRPSLTSR 576
           TVGEANRYAEDAV+RFLEAG+ AL AGANESAIVTMRPSLTSR
Sbjct: 481 TVGEANRYAEDAVRRFLEAGRAALRAGANESAIVTMRPSLTSR 522

BLAST of CmaCh02G007460.1 vs. TrEMBL
Match: F6GUS4_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_06s0004g06510 PE=4 SV=1)

HSP 1 Score: 867.1 bits (2239), Expect = 1.2e-248
Identity = 423/533 (79.36%), Postives = 471/533 (88.37%), Query Frame = 1

Query: 55  MVLRRAILTSFFLIYLLFSSQALEFDFKKRRHEIEGPIKTVVVLVMENRSFDHVLGWLKS 114
           M  RRA+L +  L+YL+FSS++L+F+   + ++I GPIKT+VVLVMENRSFDHVLGWLKS
Sbjct: 1   MASRRALLAAVVLLYLVFSSESLDFNIHGK-YKIHGPIKTLVVLVMENRSFDHVLGWLKS 60

Query: 115 VRPEIDGLTGKESNRVSVSDPNSEEIFVSSDAVFIDSDPGHSFQAISEQIFGSNDSSANP 174
            RPEIDGLTGKESNRV+ SDP+S EI+VS DA+F+DSDPGHSFQAI EQ+FG+ D   NP
Sbjct: 61  ARPEIDGLTGKESNRVNASDPDSPEIWVSDDALFVDSDPGHSFQAIREQVFGTGDWFQNP 120

Query: 175 APMNGFAQQAASMEAVDMPTTVMSGFKPELVPVYTELANQFAIFDRWFASVPASTQPNRF 234
           APM+GFAQQA +M    +  TVMSGFKP ++PVYT LAN+FA+FDRWFA+VPASTQPNRF
Sbjct: 121 APMSGFAQQAKNMSE-GLSKTVMSGFKPAVLPVYTALANEFAVFDRWFAAVPASTQPNRF 180

Query: 235 YVHSATSHGAMSNVRKDLIHGFPQKTIFDSLDENGLSFGIYYQNIPATLFFKSLRRLKHI 294
           YVHSATSHGA SNVRKDLIHGFPQ+TIFDSLDEN LSFGIYYQNIPATLFFKSLR+L+H+
Sbjct: 181 YVHSATSHGATSNVRKDLIHGFPQRTIFDSLDENDLSFGIYYQNIPATLFFKSLRKLRHV 240

Query: 295 VKFHSYAPKFKLHAKLGRLPNYAVIEQRYFDVNLFPANDDHPSHDVARGQQFVKEVYEIL 354
            +FH YA KFKLHAK G+LPNY VIEQRYFDV  FPANDDHPSHDVA GQ+FVKEVYEIL
Sbjct: 241 TRFHDYALKFKLHAKRGKLPNYVVIEQRYFDVKEFPANDDHPSHDVAIGQKFVKEVYEIL 300

Query: 355 RASPQWKEMALLITYDEHGGFYDHVPTPVSGVPSPDGIIGPDPYYFRFDRLGVRVPTILV 414
           R+SPQWKEMALLITYDEHGGFYDHVPTPVSGVP+PDGIIGPDP+YFRFDRLGVRVPTILV
Sbjct: 301 RSSPQWKEMALLITYDEHGGFYDHVPTPVSGVPNPDGIIGPDPFYFRFDRLGVRVPTILV 360

Query: 415 SPWVEKGTVIREPVGPKSTSQFEHSSIPATVKNLFNLKSNFLTKRDAWAGTFEHYLKLRD 474
           SPW+EKGTVI EP GP   SQFEHSSIPATVK LFNLKSNFLTKRDAWAGTFE+Y   RD
Sbjct: 361 SPWIEKGTVIHEPTGPTPHSQFEHSSIPATVKKLFNLKSNFLTKRDAWAGTFENYFYFRD 420

Query: 475 SPRNDCPETLPKVTAPLRPWGPKEHAKLSEFQVELIQLASQLNGDYVLNSYPNIGKYMTV 534
           +PR+DCPETLP+VT PLRP GPKE   LSEFQVELIQLASQLNGDYVLN+YP IGK MTV
Sbjct: 421 TPRDDCPETLPEVTTPLRPRGPKEDLSLSEFQVELIQLASQLNGDYVLNTYPYIGKSMTV 480

Query: 535 GEANRYAEDAVKRFLEAGKVALMAGANESAIVTMRPSLTSRTADVHSGQFIET 588
           GEANRYAEDAV+RFLEAGK AL AGAN+SAIVTMRPSLTSRT      +++ET
Sbjct: 481 GEANRYAEDAVRRFLEAGKAALRAGANDSAIVTMRPSLTSRTMGPDYKKYVET 531

BLAST of CmaCh02G007460.1 vs. TrEMBL
Match: A0A061GWF6_THECC (Non-specific phospholipase C1 OS=Theobroma cacao GN=TCM_041633 PE=4 SV=1)

HSP 1 Score: 856.7 bits (2212), Expect = 1.7e-245
Identity = 416/519 (80.15%), Postives = 457/519 (88.05%), Query Frame = 1

Query: 58  RRAILTSFFLIYLLFSSQALEFDFKKR-RHEIEGPIKTVVVLVMENRSFDHVLGWLKSVR 117
           RR  +T     YL+ SS +L  ++  R +H+I+GPIKT+V+LVMENRSFDH+LGWLKS R
Sbjct: 5   RRIPVTLILFCYLIISSHSLSSNYNFRTKHKIKGPIKTIVILVMENRSFDHILGWLKSTR 64

Query: 118 PEIDGLTGKESNRVSVSDPNSEEIFVSSDAVFIDSDPGHSFQAISEQIFGSNDSSANPAP 177
           P+IDGLTG ESN V+V+DPNS EI VS DA F+DSDPGHSFQAI EQIFGSNDSSANPAP
Sbjct: 65  PDIDGLTGSESNPVNVTDPNSPEISVSDDAFFVDSDPGHSFQAIREQIFGSNDSSANPAP 124

Query: 178 MNGFAQQAASMEAVDMPTTVMSGFKPELVPVYTELANQFAIFDRWFASVPASTQPNRFYV 237
           MNGF QQA SM    M  TVMSGFKP  +PVYT+LAN+F +FDRWFASVPASTQPNRFYV
Sbjct: 125 MNGFVQQAESM-GDGMGKTVMSGFKPGRLPVYTKLANEFGVFDRWFASVPASTQPNRFYV 184

Query: 238 HSATSHGAMSNVRKDLIHGFPQKTIFDSLDENGLSFGIYYQNIPATLFFKSLRRLKHIVK 297
           HSATS GAMSNV+KDLI+GFPQKTIFDSLDENGLSFGIYYQNIPATLFFKSLR+LKH+ K
Sbjct: 185 HSATSFGAMSNVKKDLINGFPQKTIFDSLDENGLSFGIYYQNIPATLFFKSLRKLKHLTK 244

Query: 298 FHSYAPKFKLHAKLGRLPNYAVIEQRYFDVNLFPANDDHPSHDVARGQQFVKEVYEILRA 357
           FHSYA KF LHA+LGRLPNY V+EQRYFDV  FPANDDHPSHDVARGQ+FVKEVYEILR 
Sbjct: 245 FHSYALKFGLHARLGRLPNYVVVEQRYFDVTAFPANDDHPSHDVARGQKFVKEVYEILRG 304

Query: 358 SPQWKEMALLITYDEHGGFYDHVPTPVSGVPSPDGIIGPDPYYFRFDRLGVRVPTILVSP 417
           SPQWKE ALLITYDEHGGFYDHVPTPVSGVP+PDGI+GPDPYYF+F+RLGVRVPTILVSP
Sbjct: 305 SPQWKETALLITYDEHGGFYDHVPTPVSGVPNPDGIVGPDPYYFKFNRLGVRVPTILVSP 364

Query: 418 WVEKGTVIREPVGPKSTSQFEHSSIPATVKNLFNLKSNFLTKRDAWAGTFEHYLKLRDSP 477
           W++K TVI EP GP   SQFEHSSIPATVK LFNL SNFLTKRDAWAGTFE+Y  LR +P
Sbjct: 365 WIDKATVIHEPPGPTPHSQFEHSSIPATVKKLFNLNSNFLTKRDAWAGTFENYFNLRKTP 424

Query: 478 RNDCPETLPKVTAPLRPWGPKEHAKLSEFQVELIQLASQLNGDYVLNSYPNIGKYMTVGE 537
           RNDCPETLP+V   LRPWGPKE A+LSEFQVEL+QLASQLNGDYVLN+YP IG+ MTVGE
Sbjct: 425 RNDCPETLPEVKTSLRPWGPKEDARLSEFQVELVQLASQLNGDYVLNTYPYIGRSMTVGE 484

Query: 538 ANRYAEDAVKRFLEAGKVALMAGANESAIVTMRPSLTSR 576
           ANRY EDAVKRF+EAGK A+ AGANESAIVTMRPSLTSR
Sbjct: 485 ANRYVEDAVKRFIEAGKAAIRAGANESAIVTMRPSLTSR 522

BLAST of CmaCh02G007460.1 vs. TAIR10
Match: AT1G07230.1 (AT1G07230.1 non-specific phospholipase C1)

HSP 1 Score: 844.0 bits (2179), Expect = 5.7e-245
Identity = 407/523 (77.82%), Postives = 458/523 (87.57%), Query Frame = 1

Query: 55  MVLRRAILTSFFLIYLLFSSQALEFDFKKRRHEIEGPIKTVVVLVMENRSFDHVLGWLKS 114
           M  RR + T     YLL SSQ++EF   ++ H+I+GPIKT+VV+VMENRSFDH+LGWLKS
Sbjct: 1   MAFRRVLTTVILFCYLLISSQSIEFKNSQKPHKIQGPIKTIVVVVMENRSFDHILGWLKS 60

Query: 115 VRPEIDGLTGKESNRVSVSDPNSEEIFVSSDAVFIDSDPGHSFQAISEQIFGSNDSSANP 174
            RPEIDGLTGKESN ++VSDPNS++IFVS DAVF+D DPGHSFQAI EQIFGSND+S +P
Sbjct: 61  TRPEIDGLTGKESNPLNVSDPNSKKIFVSDDAVFVDMDPGHSFQAIREQIFGSNDTSGDP 120

Query: 175 APMNGFAQQAASMEAVDMPTTVMSGFKPELVPVYTELANQFAIFDRWFASVPASTQPNRF 234
             MNGFAQQ+ SME   M   VMSGFKPE++PVYTELAN+F +FDRWFASVP STQPNRF
Sbjct: 121 K-MNGFAQQSESMEP-GMAKNVMSGFKPEVLPVYTELANEFGVFDRWFASVPTSTQPNRF 180

Query: 235 YVHSATSHGAMSNVRKDLIHGFPQKTIFDSLDENGLSFGIYYQNIPATLFFKSLRRLKHI 294
           YVHSATSHG  SNV+KDL+ GFPQKTIFDSLDENGLSFGIYYQNIPAT FFKSLRRLKH+
Sbjct: 181 YVHSATSHGCSSNVKKDLVKGFPQKTIFDSLDENGLSFGIYYQNIPATFFFKSLRRLKHL 240

Query: 295 VKFHSYAPKFKLHAKLGRLPNYAVIEQRYFDVNLFPANDDHPSHDVARGQQFVKEVYEIL 354
           VKFHSYA KFKL AKLG+LPNY+V+EQRYFD++LFPANDDHPSHDVA GQ+FVKEVYE L
Sbjct: 241 VKFHSYALKFKLDAKLGKLPNYSVVEQRYFDIDLFPANDDHPSHDVAAGQRFVKEVYETL 300

Query: 355 RASPQWKEMALLITYDEHGGFYDHVPTPVSGVPSPDGIIGPDPYYFRFDRLGVRVPTILV 414
           R+SPQWKEMALLITYDEHGGFYDHVPTPV GVP+PDGIIGPDP+YF FDRLGVRVPT L+
Sbjct: 301 RSSPQWKEMALLITYDEHGGFYDHVPTPVKGVPNPDGIIGPDPFYFGFDRLGVRVPTFLI 360

Query: 415 SPWVEKGTVIREPVGPKSTSQFEHSSIPATVKNLFNLKSNFLTKRDAWAGTFEHYLKLRD 474
           SPW+EKGTVI EP GP   SQFEHSSIPATVK LFNLKS+FLTKRDAWAGTFE Y ++RD
Sbjct: 361 SPWIEKGTVIHEPEGPTPHSQFEHSSIPATVKKLFNLKSHFLTKRDAWAGTFEKYFRIRD 420

Query: 475 SPRNDCPETLPKVTAPLRPWGPKEHAKLSEFQVELIQLASQLNGDYVLNSYPNIGKYMTV 534
           SPR DCPE LP+V   LRPWG KE +KLSEFQVELIQLASQL GD++LNSYP+IGK MTV
Sbjct: 421 SPRQDCPEKLPEVKLSLRPWGAKEDSKLSEFQVELIQLASQLVGDHLLNSYPDIGKNMTV 480

Query: 535 GEANRYAEDAVKRFLEAGKVALMAGANESAIVTMRPSLTSRTA 578
            E N+YAEDAV++FLEAG  AL AGA+E+ IVTMRPSLT+RT+
Sbjct: 481 SEGNKYAEDAVQKFLEAGMAALEAGADENTIVTMRPSLTTRTS 521

BLAST of CmaCh02G007460.1 vs. TAIR10
Match: AT2G26870.1 (AT2G26870.1 non-specific phospholipase C2)

HSP 1 Score: 635.2 bits (1637), Expect = 4.0e-182
Identity = 311/488 (63.73%), Postives = 382/488 (78.28%), Query Frame = 1

Query: 91  PIKTVVVLVMENRSFDHVLGWLKSVRPEIDGLTGKESNRVSVSDPNSEEIFVSSDAVFID 150
           PIKT+VV+VMENRSFDH+LGW+K + PEI+G+ G ESN VSVSDP+S +I   S + ++D
Sbjct: 26  PIKTIVVVVMENRSFDHMLGWMKKLNPEINGVDGSESNPVSVSDPSSRKIKFGSGSHYVD 85

Query: 151 SDPGHSFQAISEQIFGSNDSSANPAPMNGFAQQAASME-AVDMPTTVMSGFKPELVPVYT 210
            DPGHSFQAI EQ+FGSND+S +P PMNGF QQA S + + +M  +VM+GF+P+ VPVY 
Sbjct: 86  PDPGHSFQAIREQVFGSNDTSMDPPPMNGFVQQAYSEDPSGNMSASVMNGFEPDKVPVYK 145

Query: 211 ELANQFAIFDRWFASVPASTQPNRFYVHSATSHGAMSNVRKDLIHGFPQKTIFDSLDENG 270
            L ++FA+FDRWFASVP+STQPNR +VHS TS GA SN    L  G+PQ+TIFD+LD+  
Sbjct: 146 SLVSEFAVFDRWFASVPSSTQPNRMFVHSGTSAGATSNNPISLAKGYPQRTIFDNLDDEE 205

Query: 271 LSFGIYYQNIPATLFFKSLRRLKHIVKFHSYAPKFKLHAKLGRLPNYAVIEQRYFDVNLF 330
            SFGIYYQNIPA LF++SLR+LK++ KFHSY   FK HAK G+LP Y VIEQRY D  L 
Sbjct: 206 FSFGIYYQNIPAVLFYQSLRKLKYVFKFHSYGNSFKDHAKQGKLPAYTVIEQRYMDTLLE 265

Query: 331 PANDDHPSHDVARGQQFVKEVYEILRASPQWKEMALLITYDEHGGFYDHVPTPVSGVPSP 390
           PA+DDHPSHDV +GQ+F+KEVYE LRASPQW E  L+ITYDEHGG++DHVPTPV  VPSP
Sbjct: 266 PASDDHPSHDVYQGQKFIKEVYETLRASPQWNETLLIITYDEHGGYFDHVPTPVRNVPSP 325

Query: 391 DGIIGPDPYYFRFDRLGVRVPTILVSPWVEKGTVIREPVG-PKSTSQFEHSSIPATVKNL 450
           DGI+GPDP+ F+F+RLG+RVPTI VSPW+EKGTV+  P G P  +S++EHSSIPATVK L
Sbjct: 326 DGIVGPDPFLFQFNRLGIRVPTIAVSPWIEKGTVVHGPNGSPFPSSEYEHSSIPATVKKL 385

Query: 451 FNLKSNFLTKRDAWAGTFEHYLKLRDSPRNDCPETLPKVTAPLRPWGPKEHAKLSEFQVE 510
           FNL S FLTKRD WAGTFE+ L++R  PR DCPETLP+    +R     E A L+EFQ E
Sbjct: 386 FNLSSPFLTKRDEWAGTFENILQIRKEPRTDCPETLPE-PVKIRMGEANEKALLTEFQQE 445

Query: 511 LIQLASQLNGDYVLNSYP-NIGKYMTVGEANRYAEDAVKRFLEAGKVALMAGANESAIVT 570
           L+QLA+ L GD +L ++P  I K MTV E  RY EDA+KRFLEAG++AL  GAN+  +V 
Sbjct: 446 LVQLAAVLKGDNMLTTFPKEISKGMTVIEGKRYMEDAMKRFLEAGRMALSMGANKEELVH 505

Query: 571 MRPSLTSR 576
           M+ SLT R
Sbjct: 506 MKTSLTGR 512

BLAST of CmaCh02G007460.1 vs. TAIR10
Match: AT3G48610.1 (AT3G48610.1 non-specific phospholipase C6)

HSP 1 Score: 599.7 bits (1545), Expect = 1.9e-171
Identity = 298/493 (60.45%), Postives = 370/493 (75.05%), Query Frame = 1

Query: 86  HEIEGPIKTVVVLVMENRSFDHVLGWLK-SVRPEIDGLTGKESNRVSVSDPNSEE-IFVS 145
           H+ + PIKTVVVLV+ENRSFDH+LGW+K SV P I+G+TG+E N V    PNS + I  +
Sbjct: 33  HQWQSPIKTVVVLVLENRSFDHLLGWMKNSVNPTINGVTGQECNPV----PNSTQTICFT 92

Query: 146 SDAVFIDSDPGHSFQAISEQIFGSNDSSANPAPMNGFAQQAASMEAVDMPTTVMSGFKPE 205
           SDA F+D DPGHSF+A+ +Q+FGS         M GF +QA SM   ++  TVM GF+PE
Sbjct: 93  SDAEFVDPDPGHSFEAVEQQVFGSGPGQI--PSMMGFVEQALSMPG-NLSETVMKGFRPE 152

Query: 206 LVPVYTELANQFAIFDRWFASVPASTQPNRFYVHSATSHGAMSNVRKDLIHGFPQKTIFD 265
            VPVY EL  +FA+FDRWF+S+P  TQPNR +V+SATSHG+ S+V+K L  G+PQKTIFD
Sbjct: 153 AVPVYAELVKEFAVFDRWFSSIPGPTQPNRLFVYSATSHGSTSHVKKQLAQGYPQKTIFD 212

Query: 266 SLDENGLSFGIYYQNIPATLFFKSLRRLKHIVKFHSYAPKFKLHAKLGRLPNYAVIEQRY 325
           SL  N + FGIY+QNIP TLF+++LR+LK+I   H Y  KFK  A  G+LP+  VIE RY
Sbjct: 213 SLHSNDIDFGIYFQNIPTTLFYRNLRQLKYIFNLHQYDLKFKKDAAKGKLPSLTVIEPRY 272

Query: 326 FDVNLFPANDDHPSHDVARGQQFVKEVYEILRASPQWKEMALLITYDEHGGFYDHVPTPV 385
           FD+   PANDDHPSHDVA GQ+ VKEVYE LR+SPQW E  L+ITYDEHGGFYDHV TP 
Sbjct: 273 FDLKGLPANDDHPSHDVANGQKLVKEVYEALRSSPQWNETLLVITYDEHGGFYDHVKTPY 332

Query: 386 SGVPSPDGIIGPDPYYFRFDRLGVRVPTILVSPWVEKGTVIREPVGPKSTSQFEHSSIPA 445
            G+P+PDG  GP P +F+FDRLGVRVPTI+VSPW++KGTV+ E  GP  +S++EHSSIPA
Sbjct: 333 VGIPNPDGNTGPAPGFFKFDRLGVRVPTIMVSPWIQKGTVVSEAKGPTESSEYEHSSIPA 392

Query: 446 TVKNLFNLKSNFLTKRDAWAGTFEHYLKLRDSPRNDCPETLPKVTAPLRPWGPKEHAKLS 505
           T+K LFNL SNFLT RDAWA TFE  +    +PR DCP TLP+V AP+R   PKE A LS
Sbjct: 393 TIKKLFNLSSNFLTHRDAWAATFEDVVSHLTTPRTDCPMTLPEV-APMRATEPKEDAALS 452

Query: 506 EFQVELIQLASQLNGDYVLNSYP-NIGKYMTVGEANRYAEDAVKRFLEAGKVALMAGANE 565
           EFQ E++QLA+ LNGD+ L+S+P  IGK MTV +A+ Y + A  RF+ A K A+  GA++
Sbjct: 453 EFQGEVVQLAAVLNGDHFLSSFPEEIGKKMTVKQAHEYVKGATSRFIRASKEAMKLGADK 512

Query: 566 SAIVTMRPSLTSR 576
           SAIV MR SLT+R
Sbjct: 513 SAIVDMRSSLTTR 517

BLAST of CmaCh02G007460.1 vs. TAIR10
Match: AT3G03520.1 (AT3G03520.1 non-specific phospholipase C3)

HSP 1 Score: 551.6 bits (1420), Expect = 5.9e-157
Identity = 272/491 (55.40%), Postives = 347/491 (70.67%), Query Frame = 1

Query: 91  PIKTVVVLVMENRSFDHVLGWLKSVRPEIDGLTGKE--SNRVSVSDPNSEEIFVSSDAVF 150
           PIKT+VVLV ENRSFDH+LGW K + PEIDG++  E  SN +S SDPNS +IF   ++  
Sbjct: 15  PIKTIVVLVQENRSFDHMLGWFKELNPEIDGVSESEPRSNPLSTSDPNSAQIFFGKESQN 74

Query: 151 IDSDPGHSFQAISEQIFGSNDSSANPAP---MNGFAQQAASMEAVDMPTTVMSGFKPELV 210
           ID DPGHSFQAI EQ+FG   S  +P P   MNGF Q A ++        VM GF PE +
Sbjct: 75  IDPDPGHSFQAIYEQVFGKPFSDESPYPDPKMNGFVQNAEAITKGMSEKVVMQGFPPEKL 134

Query: 211 PVYTELANQFAIFDRWFASVPASTQPNRFYVHSATSHGAMSNVRKDLIHGFPQKTIFDSL 270
           PV+ EL  +FA+ DRWF+S+P+STQPNR YVH+ATS+GA SN    L+ GFPQ+T+F+SL
Sbjct: 135 PVFKELVQEFAVCDRWFSSLPSSTQPNRLYVHAATSNGAFSNDTNTLVRGFPQRTVFESL 194

Query: 271 DENGLSFGIYYQNIPATLFFKSLRRLKHIVKFHSYAPKFKLHAKLGRLPNYAVIEQRYFD 330
           +E+G +FGIYYQ+ P  LF++++R+LK++  FH Y   FK H K G+LPNY VIE RYF 
Sbjct: 195 EESGFTFGIYYQSFPNCLFYRNMRKLKYVDNFHQYHLSFKRHCKEGKLPNYVVIEPRYFK 254

Query: 331 VNLFPANDDHPSHDVARGQQFVKEVYEILRASPQWKEMALLITYDEHGGFYDHVPTPVSG 390
           +   PANDDHP +DV  GQ  VKE+YE LRASPQW E+  ++ YDEHGG+YDHVPTPV G
Sbjct: 255 ILSAPANDDHPKNDVVEGQNLVKEIYEALRASPQWNEILFVVVYDEHGGYYDHVPTPVIG 314

Query: 391 VPSPDGIIGPDPYYFRFDRLGVRVPTILVSPWVEKGTVIREPVGPKSTSQFEHSSIPATV 450
           VP+PDG++GP+PY F+FDRLGVRVP +L+SPW+E GTV+ EP GP+ TSQFEHSSIPAT+
Sbjct: 315 VPNPDGLVGPEPYNFKFDRLGVRVPALLISPWIEPGTVLHEPNGPEPTSQFEHSSIPATL 374

Query: 451 KNLFNLKSNFLTKRDAWAGTFEHYLKLRDSPRNDCPETLPKV--TAPLRPWGPKEHAKLS 510
           K +FNLKS FLTKRD WAGT +  +  R SPR DCP TLP++     +     +E   L+
Sbjct: 375 KKIFNLKS-FLTKRDEWAGTLDAVIN-RTSPRTDCPVTLPELPRARDIDIGTQEEDEDLT 434

Query: 511 EFQVELIQLASQLNGDYVLNSYP-NIGKYMTVGEANRYAEDAVKRFLEAGKVALMAGANE 570
           +FQ+ELIQ A+ L GD++ + YP  +   M V +A RY E+A  RF    K A   G +E
Sbjct: 435 DFQIELIQAAAVLKGDHIKDIYPFKLADKMKVLDAARYVEEAFTRFHGESKKAKEEGRDE 494

Query: 571 SAIVTMRPSLT 574
             IV +    T
Sbjct: 495 HEIVDLSKGST 503

BLAST of CmaCh02G007460.1 vs. TAIR10
Match: AT3G03530.1 (AT3G03530.1 non-specific phospholipase C4)

HSP 1 Score: 550.1 bits (1416), Expect = 1.7e-156
Identity = 282/485 (58.14%), Postives = 343/485 (70.72%), Query Frame = 1

Query: 91  PIKTVVVLVMENRSFDHVLGWLKSVRPEIDGLTGKE--SNRVSVSDPNSEEIFVSSDAVF 150
           PIKT+VVLV ENRSFDH LGW K +  EIDG+T  +  SN VS SD NS  +     + +
Sbjct: 13  PIKTIVVLVQENRSFDHTLGWFKELNREIDGVTKSDPKSNTVSSSDTNSLRVVFGDQSQY 72

Query: 151 IDSDPGHSFQAISEQIFGSNDSSANPAP------MNGFAQQAASMEAVDMPTTVMSGFKP 210
           ++ DPGHS Q I EQ+FG    S  P P      M+GFAQ  A      M + VM+GFKP
Sbjct: 73  VNPDPGHSIQDIYEQVFGKPWDSGKPDPNPGHPNMSGFAQN-AERNKKGMSSAVMNGFKP 132

Query: 211 ELVPVYTELANQFAIFDRWFASVPASTQPNRFYVHSATSHGAMSNVRKDLIHGFPQKTIF 270
             +PVY EL   FAI DRWFASVPASTQPNR YVHSATSHGA SN +K L+ GFPQKTIF
Sbjct: 133 NALPVYKELVQNFAICDRWFASVPASTQPNRLYVHSATSHGATSNDKKLLLEGFPQKTIF 192

Query: 271 DSLDENGLSFGIYYQNIPATLFFKSLRRLKHIVKFHSYAPKFKLHAKLGRLPNYAVIEQR 330
           +SLDE G SFGIYYQ  P+TLF+++LR+LK++  FH Y  +FK   K G+LPNY V+EQR
Sbjct: 193 ESLDEAGFSFGIYYQFPPSTLFYRNLRKLKYLTHFHQYGIQFKKDCKEGKLPNYVVVEQR 252

Query: 331 YFDVNLFPANDDHPSHDVARGQQFVKEVYEILRASPQWKEMALLITYDEHGGFYDHVPTP 390
           +FD+   PANDDHPSHDV+ GQ+ VKEVYE LR+SPQW E+  +ITYDEHGGFYDHVPTP
Sbjct: 253 WFDLLSTPANDDHPSHDVSEGQKLVKEVYEALRSSPQWNEILFIITYDEHGGFYDHVPTP 312

Query: 391 VSGVPSPDGIIGPDPYYFRFDRLGVRVPTILVSPWVEKGTVIREPVGPKSTSQFEHSSIP 450
           V GVP+PDGI+GP PY F F+RLGVRVPT  +SPW+E GTVI  P GP   SQ+EHSSIP
Sbjct: 313 VDGVPNPDGILGPPPYNFEFNRLGVRVPTFFISPWIEPGTVIHGPNGPYPRSQYEHSSIP 372

Query: 451 ATVKNLFNLKSNFLTKRDAWAGTFEHYLKLRDSPRNDCPETLPKVTAPLRPWGPKEHAKL 510
           ATVK +F LK +FL+KRD+WAGTFE  +  RDSPR DCPETL      LR    KE+A+L
Sbjct: 373 ATVKTIFKLK-DFLSKRDSWAGTFESVI-TRDSPRQDCPETL-STPIKLRGTMAKENAQL 432

Query: 511 SEFQVELIQLASQLNGDYVLNS-YPNIGKYMTVGEANRYAEDAVKRFLEAGKVALMAGAN 567
           SEFQ +L+ +A+ L GDY        + K   V +A++Y  +A ++FLE  + A   G +
Sbjct: 433 SEFQEDLVIMAAGLKGDYKNEELIHKLCKETCVADASKYVTNAFEKFLEESRKARDRGCD 492

BLAST of CmaCh02G007460.1 vs. NCBI nr
Match: gi|778713797|ref|XP_004143151.2| (PREDICTED: non-specific phospholipase C1 [Cucumis sativus])

HSP 1 Score: 1016.1 bits (2626), Expect = 2.4e-293
Identity = 493/534 (92.32%), Postives = 517/534 (96.82%), Query Frame = 1

Query: 55  MVLRRAILTSFFLIYLLFSSQALEFDFKKRRHEIEGPIKTVVVLVMENRSFDHVLGWLKS 114
           MVLR A+LTSFFLIYLLFSS A EFDFKKRRHEI+GPIK+VVVLVMENRSFDHVLGWLKS
Sbjct: 1   MVLREAVLTSFFLIYLLFSSHAFEFDFKKRRHEIQGPIKSVVVLVMENRSFDHVLGWLKS 60

Query: 115 VRPEIDGLTGKESNRVSVSDPNSEEIFVSSDAVFIDSDPGHSFQAISEQIFGSNDSSANP 174
           VRPEIDGLTGKESNRVSVSDPNSEE+FVS+DA+FIDSDPGHSFQAI EQIFGSNDSS NP
Sbjct: 61  VRPEIDGLTGKESNRVSVSDPNSEEVFVSNDAIFIDSDPGHSFQAIREQIFGSNDSSVNP 120

Query: 175 APMNGFAQQAASMEAVDMPTTVMSGFKPELVPVYTELANQFAIFDRWFASVPASTQPNRF 234
           APMNGFAQQAA+M+ +DMP TVMSGFKPE VPVYTELANQFA+FDRWFASVPASTQPNRF
Sbjct: 121 APMNGFAQQAAAMDEIDMPKTVMSGFKPERVPVYTELANQFAVFDRWFASVPASTQPNRF 180

Query: 235 YVHSATSHGAMSNVRKDLIHGFPQKTIFDSLDENGLSFGIYYQNIPATLFFKSLRRLKHI 294
           YVHSATSHGAMSNVRKDLIHGFPQKTIFDSLDENGL+FGIYYQNIPATLFFKSLR+LKHI
Sbjct: 181 YVHSATSHGAMSNVRKDLIHGFPQKTIFDSLDENGLTFGIYYQNIPATLFFKSLRKLKHI 240

Query: 295 VKFHSYAPKFKLHAKLGRLPNYAVIEQRYFDVNLFPANDDHPSHDVARGQQFVKEVYEIL 354
           VKFHSYA KFKLHAKLGRLPNYAVIEQRYFDV+L+PANDDHPSHDVARGQ+FVKEVYEIL
Sbjct: 241 VKFHSYALKFKLHAKLGRLPNYAVIEQRYFDVDLYPANDDHPSHDVARGQKFVKEVYEIL 300

Query: 355 RASPQWKEMALLITYDEHGGFYDHVPTPVSGVPSPDGIIGPDPYYFRFDRLGVRVPTILV 414
           RASPQWKEMALLITYDEHGGFYDHVPTPV+GVP+PDGIIGPDPYYFRFDRLGVRVPTILV
Sbjct: 301 RASPQWKEMALLITYDEHGGFYDHVPTPVTGVPNPDGIIGPDPYYFRFDRLGVRVPTILV 360

Query: 415 SPWVEKGTVIREPVGPKSTSQFEHSSIPATVKNLFNLKSNFLTKRDAWAGTFEHYLKLRD 474
           SPWVEKGTVI EPVGP STSQFEHSS+PATVK LFNLKSNFLTKRDAWAGTF+HYLKLRD
Sbjct: 361 SPWVEKGTVIHEPVGPTSTSQFEHSSVPATVKKLFNLKSNFLTKRDAWAGTFDHYLKLRD 420

Query: 475 SPRNDCPETLPKVTAPLRPWGPKEHAKLSEFQVELIQLASQLNGDYVLNSYPNIGKYMTV 534
            PR+DCPETLPKVTAPLRPWGPKEHAKLSEFQVELIQLASQLNGD+VLNSYPNIGKYMTV
Sbjct: 421 HPRDDCPETLPKVTAPLRPWGPKEHAKLSEFQVELIQLASQLNGDHVLNSYPNIGKYMTV 480

Query: 535 GEANRYAEDAVKRFLEAGKVALMAGANESAIVTMRPSLTSRTADVHSGQFIETI 589
           GEAN+YAEDAVKRFLEAG+VALMAGANESAIVTMRPSLTSRT  V SG F+ETI
Sbjct: 481 GEANKYAEDAVKRFLEAGRVALMAGANESAIVTMRPSLTSRTTAVDSGPFVETI 534

BLAST of CmaCh02G007460.1 vs. NCBI nr
Match: gi|659128099|ref|XP_008464045.1| (PREDICTED: non-specific phospholipase C1 [Cucumis melo])

HSP 1 Score: 1011.9 bits (2615), Expect = 4.5e-292
Identity = 492/534 (92.13%), Postives = 516/534 (96.63%), Query Frame = 1

Query: 55  MVLRRAILTSFFLIYLLFSSQALEFDFKKRRHEIEGPIKTVVVLVMENRSFDHVLGWLKS 114
           MVL +A+LTSF LIYLLFSS ALEF+FKKRRHEI+GPIKTVVVLVMENRSFDHVLGWLKS
Sbjct: 1   MVLHKAVLTSFVLIYLLFSSHALEFNFKKRRHEIQGPIKTVVVLVMENRSFDHVLGWLKS 60

Query: 115 VRPEIDGLTGKESNRVSVSDPNSEEIFVSSDAVFIDSDPGHSFQAISEQIFGSNDSSANP 174
           VRPEIDGLTGKESNR+SVSDPNSEEIFVS+DA+FIDSDPGHSFQAI EQIFGSNDSS NP
Sbjct: 61  VRPEIDGLTGKESNRISVSDPNSEEIFVSNDAIFIDSDPGHSFQAIREQIFGSNDSSGNP 120

Query: 175 APMNGFAQQAASMEAVDMPTTVMSGFKPELVPVYTELANQFAIFDRWFASVPASTQPNRF 234
           APMNGFAQQAA+M+ +DMP TVMSGFKPE VPVYTELANQFAIFDRWFASVPASTQPNRF
Sbjct: 121 APMNGFAQQAAAMDEIDMPKTVMSGFKPERVPVYTELANQFAIFDRWFASVPASTQPNRF 180

Query: 235 YVHSATSHGAMSNVRKDLIHGFPQKTIFDSLDENGLSFGIYYQNIPATLFFKSLRRLKHI 294
           YVHSATSHGAMSNVRKDLIHGFPQKTIFDSLDENGL+FGIYYQNIPATLFFKSLR+LKH+
Sbjct: 181 YVHSATSHGAMSNVRKDLIHGFPQKTIFDSLDENGLTFGIYYQNIPATLFFKSLRKLKHV 240

Query: 295 VKFHSYAPKFKLHAKLGRLPNYAVIEQRYFDVNLFPANDDHPSHDVARGQQFVKEVYEIL 354
           VKFHSYA KFKLHAKLGRLPNYAVIEQRYFDV+L+PANDDHPSHDVARGQ+FVKEVYEIL
Sbjct: 241 VKFHSYALKFKLHAKLGRLPNYAVIEQRYFDVDLYPANDDHPSHDVARGQKFVKEVYEIL 300

Query: 355 RASPQWKEMALLITYDEHGGFYDHVPTPVSGVPSPDGIIGPDPYYFRFDRLGVRVPTILV 414
           RASPQWKEMALLITYDEHGGFYDHVPTPV+GVP+PDGIIGPDPYYFRFDRLGVRVPTILV
Sbjct: 301 RASPQWKEMALLITYDEHGGFYDHVPTPVTGVPNPDGIIGPDPYYFRFDRLGVRVPTILV 360

Query: 415 SPWVEKGTVIREPVGPKSTSQFEHSSIPATVKNLFNLKSNFLTKRDAWAGTFEHYLKLRD 474
           SPWVEKGTVI EPVGP STSQFEHSSIPATVK LFNLKSNFLTKRDAWAGTF+HYLKLRD
Sbjct: 361 SPWVEKGTVIHEPVGPTSTSQFEHSSIPATVKKLFNLKSNFLTKRDAWAGTFDHYLKLRD 420

Query: 475 SPRNDCPETLPKVTAPLRPWGPKEHAKLSEFQVELIQLASQLNGDYVLNSYPNIGKYMTV 534
            PR+DCPETLPKVTAPLRPWGPKEHAKLSEFQVELIQLASQLNGD+VLNSYPNIGKYMTV
Sbjct: 421 HPRDDCPETLPKVTAPLRPWGPKEHAKLSEFQVELIQLASQLNGDHVLNSYPNIGKYMTV 480

Query: 535 GEANRYAEDAVKRFLEAGKVALMAGANESAIVTMRPSLTSRTADVHSGQFIETI 589
           GEAN+YAEDAVKRFLEAG+VALMAGANESAIVTMRPSLTSR   V SGQF+E I
Sbjct: 481 GEANKYAEDAVKRFLEAGRVALMAGANESAIVTMRPSLTSRITAVDSGQFVEAI 534

BLAST of CmaCh02G007460.1 vs. NCBI nr
Match: gi|703130545|ref|XP_010104638.1| (Phospholipase C 4 [Morus notabilis])

HSP 1 Score: 894.0 bits (2309), Expect = 1.4e-256
Identity = 435/533 (81.61%), Postives = 475/533 (89.12%), Query Frame = 1

Query: 55  MVLRRAILTSFFLIYLLFSSQALEFDFKKRRHEIEGPIKTVVVLVMENRSFDHVLGWLKS 114
           M  RR  L S   +YL+ SS +L     +R+H+I+GPIKTVVVLVMENRSFDHVLGWLKS
Sbjct: 1   MGFRRTSLASIIFLYLMISSHSLHA-LSRRKHKIQGPIKTVVVLVMENRSFDHVLGWLKS 60

Query: 115 VRPEIDGLTGKESNRVSVSDPNSEEIFVSSDAVFIDSDPGHSFQAISEQIFGSNDSSANP 174
            RPEIDGLTGKE NR++VSDP S  +FVS DA+F+DSDPGHSFQAI EQIFGSN  S NP
Sbjct: 61  TRPEIDGLTGKEWNRINVSDPKSPNVFVSDDAIFVDSDPGHSFQAIREQIFGSNHGSENP 120

Query: 175 APMNGFAQQAASMEAVDMPTTVMSGFKPELVPVYTELANQFAIFDRWFASVPASTQPNRF 234
           APMNGFAQQA SM    MP TVMSGFKP  VPVYTELAN+FA+FDRWFASVPASTQPNRF
Sbjct: 121 APMNGFAQQAVSM-GEGMPRTVMSGFKPGRVPVYTELANEFAVFDRWFASVPASTQPNRF 180

Query: 235 YVHSATSHGAMSNVRKDLIHGFPQKTIFDSLDENGLSFGIYYQNIPATLFFKSLRRLKHI 294
           YVHSATSHGA SNVRKDLIHGFPQKTIFDSLDENGL FGIYYQNIPATLFFKSLR+LKH 
Sbjct: 181 YVHSATSHGATSNVRKDLIHGFPQKTIFDSLDENGLDFGIYYQNIPATLFFKSLRKLKHA 240

Query: 295 VKFHSYAPKFKLHAKLGRLPNYAVIEQRYFDVNLFPANDDHPSHDVARGQQFVKEVYEIL 354
           +KFH+YA KFKLHA+LG+LPNY V+EQRYFDV+LFPANDDHPSHDVA GQ+FVK+VYEIL
Sbjct: 241 IKFHNYALKFKLHARLGKLPNYVVLEQRYFDVDLFPANDDHPSHDVALGQRFVKDVYEIL 300

Query: 355 RASPQWKEMALLITYDEHGGFYDHVPTPVSGVPSPDGIIGPDPYYFRFDRLGVRVPTILV 414
           RASPQWKEMALLITYDEHGGFYDHVPTPVSGVPSPDGI+GPDP+YFRFDRLGVRVPTILV
Sbjct: 301 RASPQWKEMALLITYDEHGGFYDHVPTPVSGVPSPDGIVGPDPFYFRFDRLGVRVPTILV 360

Query: 415 SPWVEKGTVIREPVGPKSTSQFEHSSIPATVKNLFNLKSNFLTKRDAWAGTFEHYLKLRD 474
           SPWV+KGTVI EPVGP   SQFEHSSIPATVK LFNLKSNFLTKRDAWAGTFE+Y KLRD
Sbjct: 361 SPWVDKGTVIHEPVGPTPHSQFEHSSIPATVKKLFNLKSNFLTKRDAWAGTFENYFKLRD 420

Query: 475 SPRNDCPETLPKVTAPLRPWGPKEHAKLSEFQVELIQLASQLNGDYVLNSYPNIGKYMTV 534
           SPR+DCPETLP+V   LRPWGPKE A+LSEFQVELIQLASQLNGDY+LN+YPNIGK MTV
Sbjct: 421 SPRDDCPETLPEVKLSLRPWGPKEDARLSEFQVELIQLASQLNGDYMLNTYPNIGKTMTV 480

Query: 535 GEANRYAEDAVKRFLEAGKVALMAGANESAIVTMRPSLTSRTADVHSGQFIET 588
           GEAN+YAEDAVKRFLEAG+ AL AGAN++AIVTMRPSLTSR +   +  ++ET
Sbjct: 481 GEANKYAEDAVKRFLEAGRAALKAGANDTAIVTMRPSLTSRISTGGTRNYLET 531

BLAST of CmaCh02G007460.1 vs. NCBI nr
Match: gi|743876371|ref|XP_011035177.1| (PREDICTED: non-specific phospholipase C1-like [Populus euphratica])

HSP 1 Score: 888.3 bits (2294), Expect = 7.5e-255
Identity = 429/523 (82.03%), Postives = 473/523 (90.44%), Query Frame = 1

Query: 55  MVLRRAILTSFFLIYLLFSSQALEFDFKKRRHE--IEGPIKTVVVLVMENRSFDHVLGWL 114
           M  RR  L S   +YL+FSSQ+L+FD   +RH+  I+GPIKT+VVLVMENRSFDH+LGWL
Sbjct: 1   MTSRRLSLASLLFLYLVFSSQSLDFDDLHKRHKPRIKGPIKTLVVLVMENRSFDHLLGWL 60

Query: 115 KSVRPEIDGLTGKESNRVSVSDPNSEEIFVSSDAVFIDSDPGHSFQAISEQIFGSNDSSA 174
           KS RP+IDGL+G ESNR+S SDPNS+E+FVS DAVFIDSDPGHSFQAI EQIFGSNDS A
Sbjct: 61  KSTRPDIDGLSGSESNRISASDPNSDEVFVSDDAVFIDSDPGHSFQAIREQIFGSNDSFA 120

Query: 175 NPAPMNGFAQQAASMEAVDMPTTVMSGFKPELVPVYTELANQFAIFDRWFASVPASTQPN 234
           +PAPMNGF QQA SM    M  TVMSGFKP L+PVYTELAN+FA+FDRWFASVPASTQPN
Sbjct: 121 DPAPMNGFVQQAKSMGET-MSKTVMSGFKPSLLPVYTELANEFAVFDRWFASVPASTQPN 180

Query: 235 RFYVHSATSHGAMSNVRKDLIHGFPQKTIFDSLDENGLSFGIYYQNIPATLFFKSLRRLK 294
           RFYVHSATSHGAMSNVRKDLIHGFPQ+TIFDSLD+NGLSFGIYYQNIPATLFFKSLR+LK
Sbjct: 181 RFYVHSATSHGAMSNVRKDLIHGFPQRTIFDSLDDNGLSFGIYYQNIPATLFFKSLRKLK 240

Query: 295 HIVKFHSYAPKFKLHAKLGRLPNYAVIEQRYFDVNLFPANDDHPSHDVARGQQFVKEVYE 354
           H++KFHSY  KFKLHAKLG+LPNY V+EQRYFDV LFPANDDHPSHDVARGQ+FVKEVYE
Sbjct: 241 HLLKFHSYELKFKLHAKLGKLPNYVVVEQRYFDVELFPANDDHPSHDVARGQRFVKEVYE 300

Query: 355 ILRASPQWKEMALLITYDEHGGFYDHVPTPVSGVPSPDGIIGPDPYYFRFDRLGVRVPTI 414
           ILR+SPQWKEMALLITYDEHGGFYDHVPTPV GVP+PDGIIGPDPYYF+FDRLG+RVPT 
Sbjct: 301 ILRSSPQWKEMALLITYDEHGGFYDHVPTPVRGVPNPDGIIGPDPYYFQFDRLGIRVPTF 360

Query: 415 LVSPWVEKGTVIREPVGPKSTSQFEHSSIPATVKNLFNLKSNFLTKRDAWAGTFEHYLKL 474
           L+SPWV+KGTVI EP GP+  SQFEHSSIPATVK LFNL SNFLT+RDAWAGTFE+Y  L
Sbjct: 361 LISPWVDKGTVIHEPDGPRPDSQFEHSSIPATVKKLFNLNSNFLTRRDAWAGTFENYFYL 420

Query: 475 RDSPRNDCPETLPKVTAPLRPWGPKEHAKLSEFQVELIQLASQLNGDYVLNSYPNIGKYM 534
           RD+PR+DCPETLP VT  LRPWGPKE A LSEFQVE+IQLASQLNGD+VLN+YP+IGK M
Sbjct: 421 RDTPRDDCPETLPDVTTSLRPWGPKEDASLSEFQVEMIQLASQLNGDHVLNAYPDIGKSM 480

Query: 535 TVGEANRYAEDAVKRFLEAGKVALMAGANESAIVTMRPSLTSR 576
           TVGEANRYAEDAV+RFLEAG+ AL AGANESAIVTM+PSLTSR
Sbjct: 481 TVGEANRYAEDAVRRFLEAGRAALRAGANESAIVTMKPSLTSR 522

BLAST of CmaCh02G007460.1 vs. NCBI nr
Match: gi|224059300|ref|XP_002299814.1| (phospholipase family protein [Populus trichocarpa])

HSP 1 Score: 885.2 bits (2286), Expect = 6.4e-254
Identity = 428/532 (80.45%), Postives = 476/532 (89.47%), Query Frame = 1

Query: 55  MVLRRAILTSFFLIYLLFSSQALEFDFKKRRHE--IEGPIKTVVVLVMENRSFDHVLGWL 114
           M  RR  LTS   +YL+ SSQ+L+FD   +RH+  I+GPIKT+VVLVMENRSFDHVLGWL
Sbjct: 1   MTSRRLSLTSLLFLYLVVSSQSLDFDDLHKRHKPKIKGPIKTLVVLVMENRSFDHVLGWL 60

Query: 115 KSVRPEIDGLTGKESNRVSVSDPNSEEIFVSSDAVFIDSDPGHSFQAISEQIFGSNDSSA 174
           KS RP+IDGL+G ESNR+S SD NS+E+FVS DAVFIDSDPGHSFQAI EQIFGSNDS A
Sbjct: 61  KSTRPDIDGLSGSESNRISASDRNSDEVFVSDDAVFIDSDPGHSFQAIREQIFGSNDSFA 120

Query: 175 NPAPMNGFAQQAASMEAVDMPTTVMSGFKPELVPVYTELANQFAIFDRWFASVPASTQPN 234
           +PAPMNGF QQA SM    M  TVMSGFKP L+PVYTELAN+FA+FDRWFASVPASTQPN
Sbjct: 121 DPAPMNGFVQQAKSMGET-MSKTVMSGFKPSLLPVYTELANEFAVFDRWFASVPASTQPN 180

Query: 235 RFYVHSATSHGAMSNVRKDLIHGFPQKTIFDSLDENGLSFGIYYQNIPATLFFKSLRRLK 294
           RFYVHSATSHGAMSNVRKDLIHGFPQ+TIFDSLD+NGLSFGIYYQNIPATLFFKSLR+LK
Sbjct: 181 RFYVHSATSHGAMSNVRKDLIHGFPQRTIFDSLDDNGLSFGIYYQNIPATLFFKSLRKLK 240

Query: 295 HIVKFHSYAPKFKLHAKLGRLPNYAVIEQRYFDVNLFPANDDHPSHDVARGQQFVKEVYE 354
           H++KFHSY  KFKLHAKLG+LPNY V+EQRYFDV LFPANDDHPSHDVARGQ+FVKEVYE
Sbjct: 241 HLLKFHSYELKFKLHAKLGKLPNYVVVEQRYFDVELFPANDDHPSHDVARGQRFVKEVYE 300

Query: 355 ILRASPQWKEMALLITYDEHGGFYDHVPTPVSGVPSPDGIIGPDPYYFRFDRLGVRVPTI 414
           ILR+SPQWKEMALLITYDEHGGFYDHVPTPV GVP+PDGIIGPDPYYF+FDRLG+RVPT 
Sbjct: 301 ILRSSPQWKEMALLITYDEHGGFYDHVPTPVRGVPNPDGIIGPDPYYFQFDRLGIRVPTF 360

Query: 415 LVSPWVEKGTVIREPVGPKSTSQFEHSSIPATVKNLFNLKSNFLTKRDAWAGTFEHYLKL 474
           L+SPW++KGTVI EP GP+  SQFEHSSIPATVK LFNL SNFLT+RDAWAG+FE+Y  L
Sbjct: 361 LISPWIDKGTVIHEPDGPRPDSQFEHSSIPATVKKLFNLNSNFLTRRDAWAGSFENYFYL 420

Query: 475 RDSPRNDCPETLPKVTAPLRPWGPKEHAKLSEFQVELIQLASQLNGDYVLNSYPNIGKYM 534
           RD+PR+DCPETLP VT  LRPWGPKE A LSEFQVE+IQLASQLNGD+VLN+YP+IGK M
Sbjct: 421 RDTPRDDCPETLPDVTTSLRPWGPKEDASLSEFQVEMIQLASQLNGDHVLNAYPDIGKSM 480

Query: 535 TVGEANRYAEDAVKRFLEAGKVALMAGANESAIVTMRPSLTSRTADVHSGQF 585
           TVGEANRYAEDAV+RFLEAG+ AL AGANESAIVTM+PSLTSR     +G++
Sbjct: 481 TVGEANRYAEDAVRRFLEAGRAALRAGANESAIVTMKPSLTSRVPAGDNGRY 531