Cla021463.1 (mRNA) Watermelon (97103) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cla021463
Type	mRNA
Organism	Citrullus. lanatus (Watermelon (97103) v1)
Description	Glutathione S-transferase (AHRD V1 ***- Q8H9E5_CUCMA); contains Interpro domain(s) IPR004046 Glutathione S-transferase, C-terminal
Location	Chr5 : 3378784 .. 3379542 (+)
Sequence length	675

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACAGAAGTGAAGCTCCATGGAGCTTGGCCAAGTCCATTCAGTTACAGAGTCATTTGGGCTCTGGCTCTCAAAGGCATTCCATACGAATACATAGAAGAAGATCTCGCCAACAAATCTCCTCTGCTTTTGCAATACAATCCCGTTCACAAGAAGATTCCAGTCCTCGTCCATGCCGGAAAACCCATCTGCGAATCCTCCATCATCCTCCAGTACATCGACGATACCTGGCCAGAGAATCCCCTTTTGCCTTCCGATCCTTTTCAGAGAGCTTCCGCTCGGTTCTGGATCAAGTTCATAGAAGACAAGGTATTTTCGACTAATCAATTTCTAATTCAAACCCATCTTACATTCTTGCATTTCTAAACATTTATGGGAACTGATTTTTACCAGAGCTCGGAGATTTGGGGAGTATTTGTTGCGGAAAAGGAAGAAGATAAGGAAAAGGCGAGAAAAGCGACCTTGGAAGTGTTGAAGACGGTGGAAGAACAAGGACTCGGCGACAAGAAATTCTTCGGCGGCGATGAAATCAACATGGTGGATTTGGCGTATGGCTTCCTCGCGCGGTGGCTGCCGGTGGTTGAAGAGTTGGTGGATGTGAAAATCCTTGAACCAAATACGGTCCCTCGCCTCTATGCTTGGGCGGAGAACTTCCGGCAAGCCGCCGTGATCAGAGACAATCTGCCGGATCACGACGACATGGTGCCGCGGCTCAAGTTCAGGAGGGAGCAGTTATTGGCAATTGCGAAGAAATCATAA

mRNA sequence

ATGACAGAAGTGAAGCTCCATGGAGCTTGGCCAAGTCCATTCAGTTACAGAGTCATTTGGGCTCTGGCTCTCAAAGGCATTCCATACGAATACATAGAAGAAGATCTCGCCAACAAATCTCCTCTGCTTTTGCAATACAATCCCGTTCACAAGAAGATTCCAGTCCTCGTCCATGCCGGAAAACCCATCTGCGAATCCTCCATCATCCTCCAGTACATCGACGATACCTGGCCAGAGAATCCCCTTTTGCCTTCCGATCCTTTTCAGAGAGCTTCCGCTCGGTTCTGGATCAAGTTCATAGAAGACAAGAGCTCGGAGATTTGGGGAGTATTTGTTGCGGAAAAGGAAGAAGATAAGGAAAAGGCGAGAAAAGCGACCTTGGAAGTGTTGAAGACGGTGGAAGAACAAGGACTCGGCGACAAGAAATTCTTCGGCGGCGATGAAATCAACATGGTGGATTTGGCGTATGGCTTCCTCGCGCGGTGGCTGCCGGTGGTTGAAGAGTTGGTGGATGTGAAAATCCTTGAACCAAATACGGTCCCTCGCCTCTATGCTTGGGCGGAGAACTTCCGGCAAGCCGCCGTGATCAGAGACAATCTGCCGGATCACGACGACATGGTGCCGCGGCTCAAGTTCAGGAGGGAGCAGTTATTGGCAATTGCGAAGAAATCATAA

Coding sequence (CDS)

ATGACAGAAGTGAAGCTCCATGGAGCTTGGCCAAGTCCATTCAGTTACAGAGTCATTTGGGCTCTGGCTCTCAAAGGCATTCCATACGAATACATAGAAGAAGATCTCGCCAACAAATCTCCTCTGCTTTTGCAATACAATCCCGTTCACAAGAAGATTCCAGTCCTCGTCCATGCCGGAAAACCCATCTGCGAATCCTCCATCATCCTCCAGTACATCGACGATACCTGGCCAGAGAATCCCCTTTTGCCTTCCGATCCTTTTCAGAGAGCTTCCGCTCGGTTCTGGATCAAGTTCATAGAAGACAAGAGCTCGGAGATTTGGGGAGTATTTGTTGCGGAAAAGGAAGAAGATAAGGAAAAGGCGAGAAAAGCGACCTTGGAAGTGTTGAAGACGGTGGAAGAACAAGGACTCGGCGACAAGAAATTCTTCGGCGGCGATGAAATCAACATGGTGGATTTGGCGTATGGCTTCCTCGCGCGGTGGCTGCCGGTGGTTGAAGAGTTGGTGGATGTGAAAATCCTTGAACCAAATACGGTCCCTCGCCTCTATGCTTGGGCGGAGAACTTCCGGCAAGCCGCCGTGATCAGAGACAATCTGCCGGATCACGACGACATGGTGCCGCGGCTCAAGTTCAGGAGGGAGCAGTTATTGGCAATTGCGAAGAAATCATAA

Protein sequence

MTEVKLHGAWPSPFSYRVIWALALKGIPYEYIEEDLANKSPLLLQYNPVHKKIPVLVHAGKPICESSIILQYIDDTWPENPLLPSDPFQRASARFWIKFIEDKSSEIWGVFVAEKEEDKEKARKATLEVLKTVEEQGLGDKKFFGGDEINMVDLAYGFLARWLPVVEELVDVKILEPNTVPRLYAWAENFRQAAVIRDNLPDHDDMVPRLKFRREQLLAIAKKS

Homology

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BLAST of Cla021463 vs. Swiss-Prot
Match: GST23_MAIZE (Glutathione transferase GST 23 OS=Zea mays PE=2 SV=1)

HSP 1 Score: 204.1 bits (518), Expect = 1.6e-51
Identity = 102/218 (46.79%), Postives = 142/218 (65.14%), Query Frame = 1


Query: 4   VKLHGAWPSPFSYRVIWALALKGIPYEYIEEDLANKSPLLLQYNPVHKKIPVLVHAGKPI 63
           VK+ G W SP   RV WAL LKG+ YEY++EDLANKS  LL++NPV KK+PVLVH GKP+
Sbjct: 6   VKVLGMWASPMVIRVEWALRLKGVEYEYVDEDLANKSADLLRHNPVTKKVPVLVHDGKPV 65

Query: 64  CESSIILQYIDDTWPEN-PLLPSDPFQRASARFWIKFIEDK-SSEIWGVFVAEKEEDKEK 123
            ES+II++YID+ W    P++P DP++RA ARFW +F EDK ++ ++ +F A  E  ++ 
Sbjct: 66  AESTIIVEYIDEVWKGGYPIMPGDPYERAQARFWARFAEDKCNAALYPIFTATGEAQRKA 125

Query: 124 ARKATLEVLKTVEEQGLGDKKFFGGDEINMVDLAYGFLARWLPVVEELVDVKILEPNTVP 183
             +A  + LKT+ E  L  KKFFGGD +  +D+  G+ A WLPV+EE+    ++    +P
Sbjct: 126 VHEAQ-QCLKTL-ETALEGKKFFGGDAVGYLDIVVGWFAHWLPVIEEVTGASVVTHEELP 185

Query: 184 RLYAWAENFRQAAVIRDNLPDHDDMVPRLKFRREQLLA 220
            + AW   F    V++  LPD D ++   K RREQLL+
Sbjct: 186 LMKAWFGRFLALDVVKAALPDRDRLLAANKARREQLLS 221

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BLAST of Cla021463 vs. Swiss-Prot
Match: GSTX6_SOYBN (Probable glutathione S-transferase OS=Glycine max GN=HSP26-A PE=2 SV=1)

HSP 1 Score: 200.7 bits (509), Expect = 1.7e-50
Identity = 103/219 (47.03%), Postives = 142/219 (64.84%), Query Frame = 1


Query: 3   EVKLHGAWPSPFSYRVIWALALKGIPYEYIEEDLANKSPLLLQYNPVHKKIPVLVHAGKP 62
           +VKL G   SPF  RV  AL LKG+ Y+++EE+L NKS LLL+YNPVHKK+PV VH  +P
Sbjct: 7   DVKLLGIVGSPFVCRVQIALKLKGVEYKFLEENLGNKSDLLLKYNPVHKKVPVFVHNEQP 66

Query: 63  ICESSIILQYIDDTWPENPLLPSDPFQRASARFWIKFIEDK--SSEIWGVFVAEKEEDKE 122
           I ES +I++YID+TW  NP+LPSDP+QRA ARFW KFI+DK   +    VF  + E+++E
Sbjct: 67  IAESLVIVEYIDETWKNNPILPSDPYQRALARFWSKFIDDKIVGAVSKSVFTVD-EKERE 126

Query: 123 KARKATLEVLKTVEEQGLGDKKFFGGDEINMVDLAYGFLARWLPVVEELVDVKILEPNTV 182
           K  + T E L+ +E + L DKKFFGG+E  +VD+A  F+A W+P+ +E+  +++      
Sbjct: 127 KNVEETYEALQFLENE-LKDKKFFGGEEFGLVDIAAVFIAFWIPIFQEIAGLQLFTSEKF 186

Query: 183 PRLYAWAENFRQAAVIRDNLPDHDDMVPRLKFRREQLLA 220
           P LY W++ F     + + LP  D +    K R E L A
Sbjct: 187 PILYKWSQEFLNHPFVHEVLPPRDPLFAYFKARYESLSA 223

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BLAST of Cla021463 vs. Swiss-Prot
Match: GSTX3_TOBAC (Probable glutathione S-transferase OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 198.0 bits (502), Expect = 1.1e-49
Identity = 99/219 (45.21%), Postives = 138/219 (63.01%), Query Frame = 1


Query: 1   MTEVKLHGAWPSPFSYRVIWALALKGIPYEYIEEDLANKSPLLLQYNPVHKKIPVLVHAG 60
           M EVKL G W SPF++RV WAL LKG+ YEYIEED  NKS LLLQ NPVHKK+PVL+H G
Sbjct: 1   MAEVKLLGFWYSPFTHRVEWALKLKGVKYEYIEEDRDNKSSLLLQSNPVHKKVPVLIHNG 60

Query: 61  KPICESSIILQYIDDTWPENPLLPSDPFQRASARFWIKFIEDKSSEIWGVFVAEKEEDKE 120
           KPI ES +IL+YID+T+    +LP DP+ RA ARFW KF+ DK + +   F   K E++E
Sbjct: 61  KPIVESMVILEYIDETFEGPSILPKDPYDRALARFWSKFLGDKVAAVVNTFF-RKGEEQE 120

Query: 121 KARKATLEVLKTVEEQGLGDKKFFGGDEINMVDLAYGFLARWLPVVEELVDVKILEPNTV 180
           K ++   E+LK ++ + L DKKFF GD+    D+A   +  WL V EE   V ++     
Sbjct: 121 KGKEEVYEMLKVLDNE-LKDKKFFVGDKFGFADIAANLVGFWLGVFEEGYGVVLVTSEKF 180

Query: 181 PRLYAWAENFRQAAVIRDNLPDHDDMVPRLKFRREQLLA 220
           P    W + +   + ++++LP  D+++   + R + ++A
Sbjct: 181 PNFSRWRDEYINCSQVKESLPSRDELLAFFRARFQAVVA 217

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BLAST of Cla021463 vs. Swiss-Prot
Match: GSTX2_TOBAC (Probable glutathione S-transferase OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 196.4 bits (498), Expect = 3.2e-49
Identity = 98/219 (44.75%), Postives = 138/219 (63.01%), Query Frame = 1


Query: 1   MTEVKLHGAWPSPFSYRVIWALALKGIPYEYIEEDLANKSPLLLQYNPVHKKIPVLVHAG 60
           M EVKL G W SPFS+RV WAL +KG+ YEYIEED  NKS LLLQ NPV+KK+PVL+H G
Sbjct: 1   MAEVKLLGFWYSPFSHRVEWALKIKGVKYEYIEEDRDNKSSLLLQSNPVYKKVPVLIHNG 60

Query: 61  KPICESSIILQYIDDTWPENPLLPSDPFQRASARFWIKFIEDKSSEIWGVFVAEKEEDKE 120
           KPI ES IIL+YID+T+    +LP DP+ RA ARFW KF++DK + +   F   K E++E
Sbjct: 61  KPIVESMIILEYIDETFEGPSILPKDPYDRALARFWAKFLDDKVAAVVNTFF-RKGEEQE 120

Query: 121 KARKATLEVLKTVEEQGLGDKKFFGGDEINMVDLAYGFLARWLPVVEELVDVKILEPNTV 180
           K ++   E+LK ++ + L DKKFF GD+    D+A   +  WL V EE     +++    
Sbjct: 121 KGKEEVYEMLKVLDNE-LKDKKFFAGDKFGFADIAANLVGFWLGVFEEGYGDVLVKSEKF 180

Query: 181 PRLYAWAENFRQAAVIRDNLPDHDDMVPRLKFRREQLLA 220
           P    W + +   + + ++LP  D+++   + R + ++A
Sbjct: 181 PNFSKWRDEYINCSQVNESLPPRDELLAFFRARFQAVVA 217

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BLAST of Cla021463 vs. Swiss-Prot
Match: GSTX1_TOBAC (Probable glutathione S-transferase OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 193.0 bits (489), Expect = 3.6e-48
Identity = 102/221 (46.15%), Postives = 136/221 (61.54%), Query Frame = 1


Query: 1   MTEVKLHGAWPSPFSYRVIWALALKGIPYEYIEEDLANKSPLLLQYNPVHKKIPVLVHAG 60
           M EVKL G W SPFS RV WAL +KG+ YEYIEED  NKS LLLQ NP+HKK+PVL+H G
Sbjct: 1   MAEVKLLGFWYSPFSRRVEWALKIKGVKYEYIEEDRDNKSSLLLQSNPIHKKVPVLIHNG 60

Query: 61  KPICESSIILQYIDDTWPENPLLPSDPFQRASARFWIKFIEDKSSEIWGVFVAEKEEDKE 120
           K I ES +IL+YID+T+    +LP DP+ RA ARFW KF++DK   +   F+  K E++E
Sbjct: 61  KRIVESMVILEYIDETFEGPSILPKDPYDRALARFWAKFLDDKVPAVVKTFL-RKGEEQE 120

Query: 121 KARKATLEVLKTVEEQGLGDKKFFGGDEINMVDLAYGFLARWLPVVEELVDVKILEPNTV 180
           K ++   E+LK ++ + L DKKFF GD+    D+A   +A WL V EE   V ++     
Sbjct: 121 KDKEEVCEMLKVLDNE-LKDKKFFVGDKFGFADIAANLVAFWLGVFEEASGVVLVTSEKF 180

Query: 181 PRLYAWAENFRQAAVIRDNLPDHDDMVPRLKFRREQLLAIA 222
           P    W   +   + I+++LP  D++   L F R +  A A
Sbjct: 181 PNFCKWRGEYINCSQIKESLPPRDEL---LAFYRSRCQAAA 216

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BLAST of Cla021463 vs. TrEMBL
Match: A0A0A0L835_CUCSA (Glutathione S-transferase OS=Cucumis sativus GN=Csa_3G133380 PE=4 SV=1)

HSP 1 Score: 412.1 bits (1058), Expect = 4.2e-112
Identity = 196/224 (87.50%), Postives = 211/224 (94.20%), Query Frame = 1


Query: 1   MTEVKLHGAWPSPFSYRVIWALALKGIPYEYIEEDLANKSPLLLQYNPVHKKIPVLVHAG 60
           MTEVKL+GAWPSPFSYRVIWALALKGIPYEY+EEDL+NKS LLLQYNPVHKKIPV VHAG
Sbjct: 1   MTEVKLYGAWPSPFSYRVIWALALKGIPYEYVEEDLSNKSSLLLQYNPVHKKIPVFVHAG 60

Query: 61  KPICESSIILQYIDDTWPENPLLPSDPFQRASARFWIKFIEDKSSEIWGVFVAEKEEDKE 120
           KPICES IILQYID+TWPENPLLPSDP Q+A+ARFWIKF+EDKS  +WG+F+AE EEDKE
Sbjct: 61  KPICESMIILQYIDETWPENPLLPSDPIQKAAARFWIKFVEDKSLVMWGLFIAEGEEDKE 120

Query: 121 KARKATLEVLKTVEEQGLGDKKFFGGDEINMVDLAYGFLARWLPVVEELVDVKILEPNTV 180
           KARKATLEVLKTVEEQ LGDKKFFGG EINMVDLAYGFLARWLPV+EELVDVK+LEPNT 
Sbjct: 121 KARKATLEVLKTVEEQALGDKKFFGGHEINMVDLAYGFLARWLPVMEELVDVKVLEPNTN 180

Query: 181 PRLYAWAENFRQAAVIRDNLPDHDDMVPRLKFRREQLLAIAKKS 225
           PRLY WAENFRQAAVIRDNLPDHD+MVPRL+ RREQLL +AKKS
Sbjct: 181 PRLYVWAENFRQAAVIRDNLPDHDEMVPRLRSRREQLLEMAKKS 224

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BLAST of Cla021463 vs. TrEMBL
Match: Q8H9E5_CUCMA (Glutathione S-transferase OS=Cucurbita maxima GN=Pugc PE=2 SV=1)

HSP 1 Score: 322.4 bits (825), Expect = 4.4e-85
Identity = 152/223 (68.16%), Postives = 184/223 (82.51%), Query Frame = 1


Query: 1   MTEVKLHGAWPSPFSYRVIWALALKGIPYEYIEEDLANKSPLLLQYNPVHKKIPVLVHAG 60
           M EVKLHG WPSPFSYRVIWAL LKGIPYEY+EEDL+NKSPLLL YNPVHKKIPVLVH G
Sbjct: 1   MAEVKLHGTWPSPFSYRVIWALKLKGIPYEYVEEDLSNKSPLLLHYNPVHKKIPVLVHGG 60

Query: 61  KPICESSIILQYIDDTWPENPLLPSDPFQRASARFWIKFIEDKSSEIWGVFVAEKEEDKE 120
           KPICES IIL+YID+TWPE PLLPSDPF+RA+ARFWIKFIEDK   ++ +F A+ +E++E
Sbjct: 61  KPICESMIILEYIDETWPEKPLLPSDPFERATARFWIKFIEDKGPAVFMIFRAKSDEEQE 120

Query: 121 KARKATLEVLKTVEEQGL--GD---KKFFGGDEINMVDLAYGFLARWLPVVEELVDVKIL 180
            A+K +LE+L+TVEE G   GD   +KFFGGDEI M DLA+G++A W+ V+EE+V+VK+L
Sbjct: 121 NAKKQSLEMLRTVEEWGFRGGDGRERKFFGGDEIGMTDLAFGWIAHWIGVIEEVVNVKLL 180

Query: 181 EPNTVPRLYAWAENFRQAAVIRDNLPDHDDMVPRLKFRREQLL 219
           + N  P++YAW E F+ AAVIRDNLPD + M    + RREQLL
Sbjct: 181 DANEFPKVYAWTERFKDAAVIRDNLPDRNLMAAAFRRRREQLL 223

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BLAST of Cla021463 vs. TrEMBL
Match: B9RWR5_RICCO (Glutathione s-transferase, putative OS=Ricinus communis GN=RCOM_1023660 PE=1 SV=1)

HSP 1 Score: 309.3 bits (791), Expect = 3.8e-81
Identity = 150/224 (66.96%), Postives = 178/224 (79.46%), Query Frame = 1


Query: 1   MTEV-KLHGAWPSPFSYRVIWALALKGIPYEYIEEDLANKSPLLLQYNPVHKKIPVLVHA 60
           M EV KLHGAWPSPFS RVIWAL LKGIPYEY+EEDL NKSPLLLQYNPVHKKIPVLVH 
Sbjct: 1   MAEVLKLHGAWPSPFSCRVIWALKLKGIPYEYVEEDLFNKSPLLLQYNPVHKKIPVLVHG 60

Query: 61  GKPICESSIILQYIDDTWPENPLLPSDPFQRASARFWIKFIEDKSSEIWGVFVAEKEEDK 120
           GKPICES+IIL+Y+D+TWPENPLLPSDP +RA ARFW+KFIEDK + IW +F   K E+ 
Sbjct: 61  GKPICESTIILEYLDETWPENPLLPSDPHERAVARFWVKFIEDKGTAIWNIF-RTKGEEL 120

Query: 121 EKARKATLEVLKTVEEQGLG--DKKFFGGDEINMVDLAYGFLARWLPVVEELVDVKILEP 180
           EKA K  LEVLKT+EE  +G  D K+FGGD+I +VD+A+  +A WL V+EE+  VK+LE 
Sbjct: 121 EKAVKNCLEVLKTIEEHAMGVSDDKYFGGDKIGIVDIAFCGIAHWLGVIEEVAGVKVLES 180

Query: 181 NTVPRLYAWAENFRQAAVIRDNLPDHDDMVPRLKFRREQLLAIA 222
              PRL+AW ENF++A +I++NLPD D M    K RRE +LA A
Sbjct: 181 QKFPRLHAWTENFKEAPIIKENLPDRDQMTAFFKRRREMILASA 223

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BLAST of Cla021463 vs. TrEMBL
Match: A0A0A0L9K2_CUCSA (Glutathione S-transferase OS=Cucumis sativus GN=Csa_3G133370 PE=3 SV=1)

HSP 1 Score: 308.5 bits (789), Expect = 6.5e-81
Identity = 144/224 (64.29%), Postives = 184/224 (82.14%), Query Frame = 1


Query: 1   MTEVKLHGAWPSPFSYRVIWALALKGIPYEYIEEDLANKSPLLLQYNPVHKKIPVLVHAG 60
           M E+KLHG WPSPFSYRVIWALALKGIPY+YIEEDL+NKSPLLLQYNPVHKKIPVLVHAG
Sbjct: 1   MEELKLHGTWPSPFSYRVIWALALKGIPYQYIEEDLSNKSPLLLQYNPVHKKIPVLVHAG 60

Query: 61  KPICESSIILQYIDDTWPENPLLPSDPFQRASARFWIKFIEDKSSEIWGVFVAEKEEDKE 120
           KPI ES IIL+YI+ TWP+NPLLPSDP++RA+ARFWIKFI+DK + ++ +F A+ EE++E
Sbjct: 61  KPISESMIILEYIEQTWPQNPLLPSDPYERATARFWIKFIDDKGTVVFKIFRAKNEEERE 120

Query: 121 KARKATLEVLKTVEEQGL--GDKKFFGGDEINMVDLAYGFLARWLPVVEELVDVKILEPN 180
            A+K +LE+L+TVEE G   G++KFFGGD+I M DLA+G +A WL ++E++  VK+L+ +
Sbjct: 121 DAKKQSLEMLRTVEEWGFSGGERKFFGGDDIGMADLAFGAIAYWLGMIEQVTGVKLLDVD 180

Query: 181 TVPRLYAWAENFRQAAVIRDNLPDHDDMVPRLKFRREQLLAIAK 223
             P++  W   F++A VIRDNLPD + M+   + RREQLL  AK
Sbjct: 181 EFPQVCGWIGRFKEAPVIRDNLPDWNPMMENFRRRREQLLESAK 224

[Close]

BLAST of Cla021463 vs. TrEMBL
Match: A0A061ET20_THECC (Glutathione S-transferase tau 7 OS=Theobroma cacao GN=TCM_046764 PE=4 SV=1)

HSP 1 Score: 300.4 bits (768), Expect = 1.8e-78
Identity = 136/219 (62.10%), Postives = 178/219 (81.28%), Query Frame = 1


Query: 1   MTEVKLHGAWPSPFSYRVIWALALKGIPYEYIEEDLANKSPLLLQYNPVHKKIPVLVHAG 60
           M EVKLHG+W SPFS+RVIWAL +KG+ YEYIEEDL+NKS LLL+YNPV+KKIPVLVH G
Sbjct: 1   MAEVKLHGSWASPFSHRVIWALKIKGVDYEYIEEDLSNKSELLLKYNPVYKKIPVLVHGG 60

Query: 61  KPICESSIILQYIDDTWPENPLLPSDPFQRASARFWIKFIEDKSSEIWGVFVAEKEEDKE 120
           KPI ES +IL+YI++TWPENPLLPSDP++RA ARFWI+F+EDK       F++   E++E
Sbjct: 61  KPIAESLVILEYIEETWPENPLLPSDPYERAMARFWIQFVEDKGPIFRAFFLSTGREEQE 120

Query: 121 KARKATLEVLKTVEEQGLGDKKFFGGDEINMVDLAYGFLARWLPVVEELVDVKILEPNTV 180
           KA K  LEVLK +EE+ LG+KKFFGGD IN+VD++YG LA W   VEE++ + +LEPNT+
Sbjct: 121 KAAKELLEVLKIIEEKALGEKKFFGGDTINLVDISYGVLAYWFKNVEEVIGIMVLEPNTL 180

Query: 181 PRLYAWAENFRQAAVIRDNLPDHDDMVPRLKFRREQLLA 220
           PRL+ WA+NF++ AVI++ +PD D M+  L+  RE+++A
Sbjct: 181 PRLHEWAQNFKEVAVIKETIPDRDKMLAYLRRVREKIVA 219

[Close]

BLAST of Cla021463 vs. NCBI nr
Match: gi|659075966|ref|XP_008438427.1| (PREDICTED: probable glutathione S-transferase isoform X2 [Cucumis melo])

HSP 1 Score: 425.2 bits (1092), Expect = 6.9e-116
Identity = 202/224 (90.18%), Postives = 215/224 (95.98%), Query Frame = 1


Query: 1   MTEVKLHGAWPSPFSYRVIWALALKGIPYEYIEEDLANKSPLLLQYNPVHKKIPVLVHAG 60
           MTEVKLHGAWPSPFSYRVIWAL LKGIPYEYIEEDL+NKSPLLLQYNPVHKKIPVLVHAG
Sbjct: 1   MTEVKLHGAWPSPFSYRVIWALELKGIPYEYIEEDLSNKSPLLLQYNPVHKKIPVLVHAG 60

Query: 61  KPICESSIILQYIDDTWPENPLLPSDPFQRASARFWIKFIEDKSSEIWGVFVAEKEEDKE 120
           KPICES IILQYID+TWPENPLLPSDP QRA+ARFWIKFIEDKS EIWG+F+AE EEDKE
Sbjct: 61  KPICESMIILQYIDETWPENPLLPSDPIQRAAARFWIKFIEDKSMEIWGLFIAEGEEDKE 120

Query: 121 KARKATLEVLKTVEEQGLGDKKFFGGDEINMVDLAYGFLARWLPVVEELVDVKILEPNTV 180
           KARKATLEVLKTVEEQ LGDKKFFGGDEINMVDLAYGFLARWLPV+EELVDVK+LEPNT+
Sbjct: 121 KARKATLEVLKTVEEQALGDKKFFGGDEINMVDLAYGFLARWLPVMEELVDVKVLEPNTI 180

Query: 181 PRLYAWAENFRQAAVIRDNLPDHDDMVPRLKFRREQLLAIAKKS 225
           PRLY WAENFRQAAVIR+NLPDHD+MVPRL+ RREQ+L +AKKS
Sbjct: 181 PRLYVWAENFRQAAVIRNNLPDHDEMVPRLRSRREQVLKMAKKS 224

[Close]

BLAST of Cla021463 vs. NCBI nr
Match: gi|659075968|ref|XP_008438428.1| (PREDICTED: probable glutathione S-transferase isoform X3 [Cucumis melo])

HSP 1 Score: 418.7 bits (1075), Expect = 6.4e-114
Identity = 200/224 (89.29%), Postives = 213/224 (95.09%), Query Frame = 1


Query: 1   MTEVKLHGAWPSPFSYRVIWALALKGIPYEYIEEDLANKSPLLLQYNPVHKKIPVLVHAG 60
           MTEVKLHG WPSPFSYRVIWALALKGI YEYIEEDL+NKSPLLLQYNPVHKKIPVLVHAG
Sbjct: 1   MTEVKLHGTWPSPFSYRVIWALALKGISYEYIEEDLSNKSPLLLQYNPVHKKIPVLVHAG 60

Query: 61  KPICESSIILQYIDDTWPENPLLPSDPFQRASARFWIKFIEDKSSEIWGVFVAEKEEDKE 120
           KPI ES IILQYID+TWPENPLLPSDP QRA+ARFWIKFIEDKS EIWG+F+AE EEDKE
Sbjct: 61  KPISESMIILQYIDETWPENPLLPSDPLQRAAARFWIKFIEDKSMEIWGLFIAEGEEDKE 120

Query: 121 KARKATLEVLKTVEEQGLGDKKFFGGDEINMVDLAYGFLARWLPVVEELVDVKILEPNTV 180
           KARKATLEVLKTVEEQ LGDKKFFGGDEINMVDLAYGFLARWLPV+EELVDVK+LEPNT+
Sbjct: 121 KARKATLEVLKTVEEQALGDKKFFGGDEINMVDLAYGFLARWLPVMEELVDVKVLEPNTI 180

Query: 181 PRLYAWAENFRQAAVIRDNLPDHDDMVPRLKFRREQLLAIAKKS 225
           PRLY WAENFRQAAVIR+NLPDHD+MVPRL+ RREQ+L +AKKS
Sbjct: 181 PRLYVWAENFRQAAVIRNNLPDHDEMVPRLRSRREQVLKMAKKS 224

[Close]

BLAST of Cla021463 vs. NCBI nr
Match: gi|778678021|ref|XP_011650902.1| (PREDICTED: probable glutathione S-transferase [Cucumis sativus])

HSP 1 Score: 412.1 bits (1058), Expect = 6.0e-112
Identity = 196/224 (87.50%), Postives = 211/224 (94.20%), Query Frame = 1


Query: 1   MTEVKLHGAWPSPFSYRVIWALALKGIPYEYIEEDLANKSPLLLQYNPVHKKIPVLVHAG 60
           MTEVKL+GAWPSPFSYRVIWALALKGIPYEY+EEDL+NKS LLLQYNPVHKKIPV VHAG
Sbjct: 1   MTEVKLYGAWPSPFSYRVIWALALKGIPYEYVEEDLSNKSSLLLQYNPVHKKIPVFVHAG 60

Query: 61  KPICESSIILQYIDDTWPENPLLPSDPFQRASARFWIKFIEDKSSEIWGVFVAEKEEDKE 120
           KPICES IILQYID+TWPENPLLPSDP Q+A+ARFWIKF+EDKS  +WG+F+AE EEDKE
Sbjct: 61  KPICESMIILQYIDETWPENPLLPSDPIQKAAARFWIKFVEDKSLVMWGLFIAEGEEDKE 120

Query: 121 KARKATLEVLKTVEEQGLGDKKFFGGDEINMVDLAYGFLARWLPVVEELVDVKILEPNTV 180
           KARKATLEVLKTVEEQ LGDKKFFGG EINMVDLAYGFLARWLPV+EELVDVK+LEPNT 
Sbjct: 121 KARKATLEVLKTVEEQALGDKKFFGGHEINMVDLAYGFLARWLPVMEELVDVKVLEPNTN 180

Query: 181 PRLYAWAENFRQAAVIRDNLPDHDDMVPRLKFRREQLLAIAKKS 225
           PRLY WAENFRQAAVIRDNLPDHD+MVPRL+ RREQLL +AKKS
Sbjct: 181 PRLYVWAENFRQAAVIRDNLPDHDEMVPRLRSRREQLLEMAKKS 224

[Close]

BLAST of Cla021463 vs. NCBI nr
Match: gi|659075964|ref|XP_008438426.1| (PREDICTED: probable glutathione S-transferase isoform X1 [Cucumis melo])

HSP 1 Score: 398.3 bits (1022), Expect = 9.0e-108
Identity = 198/225 (88.00%), Postives = 207/225 (92.00%), Query Frame = 1


Query: 1   MTEVKLHGAWPSPFSYRVIWALALKGIPYEYIEEDLANKSPLLLQYNPVHKKIPVLVHAG 60
           MTEVKLHG WPSPFSYRVIWALALKGI YEYIEEDL+NKSPLLLQYNPVHKKIPVLVHAG
Sbjct: 1   MTEVKLHGTWPSPFSYRVIWALALKGISYEYIEEDLSNKSPLLLQYNPVHKKIPVLVHAG 60

Query: 61  KPICESSIILQYIDDTWPENPLLPSDPFQRASARFWIKFIEDKSSEIWGVF-VAEKEEDK 120
           KPI ES IILQYID+TWPENPLLPSDP QRA+ARFWIKFIEDKS  I GV  + E EE K
Sbjct: 61  KPISESMIILQYIDETWPENPLLPSDPLQRAAARFWIKFIEDKSLVILGVSDILEGEEYK 120

Query: 121 EKARKATLEVLKTVEEQGLGDKKFFGGDEINMVDLAYGFLARWLPVVEELVDVKILEPNT 180
           EKARKA+LEVLKTVEEQ LGDKKFFGGDEINMVDLAYGFLARWLPVVEE+V+VKILEPNT
Sbjct: 121 EKARKASLEVLKTVEEQALGDKKFFGGDEINMVDLAYGFLARWLPVVEEVVEVKILEPNT 180

Query: 181 VPRLYAWAENFRQAAVIRDNLPDHDDMVPRLKFRREQLLAIAKKS 225
           VPRLYAWAENFRQAAVIRDNLPDHD+MV RLKFRREQ L I +KS
Sbjct: 181 VPRLYAWAENFRQAAVIRDNLPDHDEMVSRLKFRREQRLQIGRKS 225

[Close]

BLAST of Cla021463 vs. NCBI nr
Match: gi|23978432|dbj|BAC21263.1| (glutathione S-transferase [Cucurbita maxima])

HSP 1 Score: 322.4 bits (825), Expect = 6.3e-85
Identity = 152/223 (68.16%), Postives = 184/223 (82.51%), Query Frame = 1


Query: 1   MTEVKLHGAWPSPFSYRVIWALALKGIPYEYIEEDLANKSPLLLQYNPVHKKIPVLVHAG 60
           M EVKLHG WPSPFSYRVIWAL LKGIPYEY+EEDL+NKSPLLL YNPVHKKIPVLVH G
Sbjct: 1   MAEVKLHGTWPSPFSYRVIWALKLKGIPYEYVEEDLSNKSPLLLHYNPVHKKIPVLVHGG 60

Query: 61  KPICESSIILQYIDDTWPENPLLPSDPFQRASARFWIKFIEDKSSEIWGVFVAEKEEDKE 120
           KPICES IIL+YID+TWPE PLLPSDPF+RA+ARFWIKFIEDK   ++ +F A+ +E++E
Sbjct: 61  KPICESMIILEYIDETWPEKPLLPSDPFERATARFWIKFIEDKGPAVFMIFRAKSDEEQE 120

Query: 121 KARKATLEVLKTVEEQGL--GD---KKFFGGDEINMVDLAYGFLARWLPVVEELVDVKIL 180
            A+K +LE+L+TVEE G   GD   +KFFGGDEI M DLA+G++A W+ V+EE+V+VK+L
Sbjct: 121 NAKKQSLEMLRTVEEWGFRGGDGRERKFFGGDEIGMTDLAFGWIAHWIGVIEEVVNVKLL 180

Query: 181 EPNTVPRLYAWAENFRQAAVIRDNLPDHDDMVPRLKFRREQLL 219
           + N  P++YAW E F+ AAVIRDNLPD + M    + RREQLL
Sbjct: 181 DANEFPKVYAWTERFKDAAVIRDNLPDRNLMAAAFRRRREQLL 223

The following BLAST results are available for this feature:

BLAST of Cla021463 vs. Swiss-Prot


Match Name	E-value	Identity	Description
GST23_MAIZE	1.6e-51	46.79	Glutathione transferase GST 23 OS=Zea mays PE=2 SV=1	[more]
GSTX6_SOYBN	1.7e-50	47.03	Probable glutathione S-transferase OS=Glycine max GN=HSP26-A PE=2 SV=1	[more]
GSTX3_TOBAC	1.1e-49	45.21	Probable glutathione S-transferase OS=Nicotiana tabacum PE=2 SV=1	[more]
GSTX2_TOBAC	3.2e-49	44.75	Probable glutathione S-transferase OS=Nicotiana tabacum PE=2 SV=1	[more]
GSTX1_TOBAC	3.6e-48	46.15	Probable glutathione S-transferase OS=Nicotiana tabacum PE=2 SV=1	[more]

BLAST of Cla021463 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L835_CUCSA	4.2e-112	87.50	Glutathione S-transferase OS=Cucumis sativus GN=Csa_3G133380 PE=4 SV=1	[more]
Q8H9E5_CUCMA	4.4e-85	68.16	Glutathione S-transferase OS=Cucurbita maxima GN=Pugc PE=2 SV=1	[more]
B9RWR5_RICCO	3.8e-81	66.96	Glutathione s-transferase, putative OS=Ricinus communis GN=RCOM_1023660 PE=1 SV=...	[more]
A0A0A0L9K2_CUCSA	6.5e-81	64.29	Glutathione S-transferase OS=Cucumis sativus GN=Csa_3G133370 PE=3 SV=1	[more]
A0A061ET20_THECC	1.8e-78	62.10	Glutathione S-transferase tau 7 OS=Theobroma cacao GN=TCM_046764 PE=4 SV=1	[more]

BLAST of Cla021463 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659075966\|ref\|XP_008438427.1\|	6.9e-116	90.18	PREDICTED: probable glutathione S-transferase isoform X2 [Cucumis melo]	[more]
gi\|659075968\|ref\|XP_008438428.1\|	6.4e-114	89.29	PREDICTED: probable glutathione S-transferase isoform X3 [Cucumis melo]	[more]
gi\|778678021\|ref\|XP_011650902.1\|	6.0e-112	87.50	PREDICTED: probable glutathione S-transferase [Cucumis sativus]	[more]
gi\|659075964\|ref\|XP_008438426.1\|	9.0e-108	88.00	PREDICTED: probable glutathione S-transferase isoform X1 [Cucumis melo]	[more]
gi\|23978432\|dbj\|BAC21263.1\|	6.3e-85	68.16	glutathione S-transferase [Cucurbita maxima]	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **INTERPRO**
Term	Definition
IPR004045	Glutathione_S-Trfase_N
IPR004046	GST_C
IPR010987	Glutathione-S-Trfase_C-like
IPR012336	Thioredoxin-like_fold

Vocabulary: **Molecular Function**
Term	Definition
GO:0005515	protein binding

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
biological_process	GO:0006090	pyruvate metabolic process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0005515	protein binding
molecular_function	GO:0004462	lactoylglutathione lyase activity
molecular_function	GO:0016740	transferase activity

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cla021463	Cla021463	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cla021463	Cla021463.1-protein	polypeptide

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cla021463.1.cds1	Cla021463.1.cds1	CDS
Cla021463.1.cds2	Cla021463.1.cds2	CDS

InterPro

Analysis Name: InterPro Annotations of watermelon (97103)
Date Performed: 2016-09-28


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004045	Glutathione S-transferase, N-terminal	PFAM	PF02798	GST_N	coord: 4..74 score: 8.8
IPR004045	Glutathione S-transferase, N-terminal	PROFILE	PS50404	GST_NTER	coord: 2..81 score: 22
IPR004046	Glutathione S-transferase, C-terminal	PFAM	PF00043	GST_C	coord: 103..190 score: 2.
IPR010987	Glutathione S-transferase, C-terminal-like	GENE3D	G3DSA:1.20.1050.10		coord: 92..217 score: 9.0
IPR010987	Glutathione S-transferase, C-terminal-like	PROFILE	PS50405	GST_CTER	coord: 86..210 score: 14
IPR010987	Glutathione S-transferase, C-terminal-like	unknown	SSF47616	GST C-terminal domain-like	coord: 75..215 score: 1.34
IPR012336	Thioredoxin-like fold	GENE3D	G3DSA:3.40.30.10		coord: 3..91 score: 6.4
IPR012336	Thioredoxin-like fold	unknown	SSF52833	Thioredoxin-like	coord: 4..102 score: 3.92
None	No IPR available	PANTHER	PTHR11260	GLUTATHIONE S-TRANSFERASE, GST, SUPERFAMILY, GST DOMAIN CONTAINING	coord: 1..220 score: 1.4E
None	No IPR available	PANTHER	PTHR11260:SF227	SUBFAMILY NOT NAMED	coord: 1..220 score: 1.4E