Cla020749.1 (mRNA) Watermelon (97103) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cla020749
Type	mRNA
Organism	Citrullus. lanatus (Watermelon (97103) v1)
Description	Cytochrome P450 (AHRD V1 -- B9MZU2_POPTR); contains Interpro domain(s) IPR001128 Cytochrome P450
Location	Chr5 : 27132213 .. 27134656 (+)
Sequence length	1107

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATCAAAGGACCTCCCTATCGTCCGATCACTGGTAACACCAACAATATCCACCGTCTGGATGCAGAGGCACAAGAGAAGGCGATGCCTTATATCCACCAGTGGTCCGAAGAGTATGGAAAGACGTTTGTGTACTGGTTTGGGTCGACACCCAGATTGACCATATCTGACCTGGATGTGATTAAGGAAGTGCTTATGAATACGAATGGGTCTTTTACAAAGACCAAGGCCAGTCGGTTTGTGAAGTTTCTGATTGGAAAAGGACTTGCCGGACTCGATGGGGAAAAATGGGCCCTCCATAGGAGGATTGCAAATCAGGCTTTCACTGTGGAGCGTGTGAAGGTTTAAAAACAAAACCTTTTTTATTTTTAAGTACTAGTCGTATAACATACTTTTTGGTCACTAGTGCATTGGTTTGCTAATTGAGTATTTTTTTTTTTTTAAATATTTTTTTGACATGCAACTTAACTTTTAATTAAGTAGTATATATTTAATGTCATTAAAATAGAGAGATTTAAACTGCCAACTTTAAAGGAAGTACATGTTAGCTTCCATTAAATTATGCTATTTTAGTTAAAAGTATTCTTTATATACTTTCTTTTTTCTCTTTGTCAAAAAGTATGATTATAAATATATTATATTTATTATGTCTAGGAGTTTTTCTTTTCTTATTTATTTCTTTTTAATTCTTATGAATAATATAGTTTTATTTGGTCACAACATGTAGGGATGAGAGATGTAAACTTTTGATCTTTTGAATAAAAATACATGTCTTAAGTAGATGAATTAGGTTGGAATTATGCTCATTTTTGAGCTCCCTTTTGGCAGAAGTAAACAAAGTTTATATATATATATATATATATCAAAACAAAGCTTAAAATTTTAAGATATGTTCAGTTCAAGTCTCTCTAAGTCTATTTTGTGTATTTAGAATTTTGAGTCATTTCAAGTACTTGTAAATATTAGATGAATTATTTTGTTTGTTTGTTTTTTTGTTTTTTTTTTTGTTTTTCCTTATAAATACATTTGTTTTCTCACATTTACCATAATTTCAAGTAAACAGTTGTTTTTCTAATGGCTTTTCAAGTAAACATTTTAAGTACTTGAAATTTTTTTTTTTTTTTTAAATTTTATTAACAAAAATTGTGTAAAAATATTTAATCCAAAATTTCACAATTTGCAGGAATGGTTACCAGAGATTAGGTTGAGTATTTATAGTGTATTGGAGAAATGGGAAGAGATGAAGGGAGGGAAGGAGGAGTTTGAATTAGATGTCCGTAAACAACTCCGTCTCCTTTCAGCAGACGTAAGTAATTTCAAGAACTGCTTTTGAAAGTAATTATAAAGAAGGCAAACACATTTTCCATTTGCAAGAAGAGCAAGTGCGTGATTTTTACCAAGACGGCACAAGTGTTTATATTCCTGGTTTCAGGTTCTATCATTTTTCTAATTTCTTAAAATTTTTATTGGGATAACCCATTACCCATAACCCATAACCCACTTTTTTTTATTGGTAAGTAATTTAATTTATCGAAATATTGAATTTCACATGGAAAAATATAGATTTTTGCCTACCAAAGCAAACAGAAAGAGGCAGTGGCTGGAAAAGGAAATTCGTGAATCAATAAAAACACTTATTGAAAGTGAGAAAAACAAGAAAGAAAGGGAAAACTCAACTAATCTATCTACCCTTTTAATGTCATCTTACAAGAACGAGAATGGAGAAGAAGAAATGCTTGGAGTGGAAGAAATCATGGATGAATACAAGACATTCTATTTTGCAGGAATGGAAACAACTGCAAATCTATTAACTTGGGCACTTCTACCACTAGCACAACATCAAGAATGGCAACACCGAGCTAGAGAAGAAGTCATCAAAGTTTGCGGCCACAAGACACCCCCTTCATCTAACCATTTAACTCAACTTAAACTGGTAACTATTAAATCACTAATCAACTCAAAAGCTTAAACTAAGATACGAGAGAGACAAGTATTTATGTTTGATGATGCATTTATTTTGACAGATGAGTATGATTATCAATGAAACACTTCTACTTTATCCTCCAGCGTTGTTGTTGGGGCCGCGGATAACAAAAGGAATGACTCTAGGAAGCATCGATGTTCCGAAAGGCACACAACTTCAAATTGTAGTGGCTGCAATCCACCACGACAAAGAGATTTGGGGAGAAGATGCAAGCGAATTCAACCCTCCGAGATTTAGTGCACCTAGGAAGCATCTGGCTTCATTTATGCCATTTGGTTTGGGTCCTAGAATTTGTGTAGGCCAACATTTGGCTTTGATTGAGGCCAAAGTTGCATTGGCTATGATAATTCAACGATTCTCTTTTACGATCTCTCCAACCTACATGCATGCCCCTGTGATGTTGGTAACCTTACAACCTCAGTTTGGTGTTCAACTCCTCATTAAGAGCTTATGGAATTGA

mRNA sequence

ATGATCAAAGGACCTCCCTATCGTCCGATCACTGGTAACACCAACAATATCCACCGTCTGGATGCAGAGGCACAAGAGAAGGCGATGCCTTATATCCACCAGTGGTCCGAAGAGTATGGAAAGACGTTTGTGTACTGGTTTGGGTCGACACCCAGATTGACCATATCTGACCTGGATGTGATTAAGGAAGTGCTTATGAATACGAATGGGTCTTTTACAAAGACCAAGGCCAGTCGGTTTGTGAAGTTTCTGATTGGAAAAGGACTTGCCGGACTCGATGGGGAAAAATGGGCCCTCCATAGGAGGATTGCAAATCAGGCTTTCACTGTGGAGCGTGTGAAGGAATGGTTACCAGAGATTAGGTTGAGTATTTATAGTGTATTGGAGAAATGGGAAGAGATGAAGGGAGGGAAGGAGGAGTTTGAATTAGATGTCCGTAAACAACTCCGTCTCCTTTCAGCAGACAACGAGAATGGAGAAGAAGAAATGCTTGGAGTGGAAGAAATCATGGATGAATACAAGACATTCTATTTTGCAGGAATGGAAACAACTGCAAATCTATTAACTTGGGCACTTCTACCACTAGCACAACATCAAGAATGGCAACACCGAGCTAGAGAAGAAGTCATCAAAGTTTGCGGCCACAAGACACCCCCTTCATCTAACCATTTAACTCAACTTAAACTGATGAGTATGATTATCAATGAAACACTTCTACTTTATCCTCCAGCGTTGTTGTTGGGGCCGCGGATAACAAAAGGAATGACTCTAGGAAGCATCGATGTTCCGAAAGGCACACAACTTCAAATTGTAGTGGCTGCAATCCACCACGACAAAGAGATTTGGGGAGAAGATGCAAGCGAATTCAACCCTCCGAGATTTAGTGCACCTAGGAAGCATCTGGCTTCATTTATGCCATTTGGTTTGGGTCCTAGAATTTGTGTAGGCCAACATTTGGCTTTGATTGAGGCCAAAGTTGCATTGGCTATGATAATTCAACGATTCTCTTTTACGATCTCTCCAACCTACATGCATGCCCCTGTGATGTTGGTAACCTTACAACCTCAGTTTGGTGTTCAACTCCTCATTAAGAGCTTATGGAATTGA

Coding sequence (CDS)

ATGATCAAAGGACCTCCCTATCGTCCGATCACTGGTAACACCAACAATATCCACCGTCTGGATGCAGAGGCACAAGAGAAGGCGATGCCTTATATCCACCAGTGGTCCGAAGAGTATGGAAAGACGTTTGTGTACTGGTTTGGGTCGACACCCAGATTGACCATATCTGACCTGGATGTGATTAAGGAAGTGCTTATGAATACGAATGGGTCTTTTACAAAGACCAAGGCCAGTCGGTTTGTGAAGTTTCTGATTGGAAAAGGACTTGCCGGACTCGATGGGGAAAAATGGGCCCTCCATAGGAGGATTGCAAATCAGGCTTTCACTGTGGAGCGTGTGAAGGAATGGTTACCAGAGATTAGGTTGAGTATTTATAGTGTATTGGAGAAATGGGAAGAGATGAAGGGAGGGAAGGAGGAGTTTGAATTAGATGTCCGTAAACAACTCCGTCTCCTTTCAGCAGACAACGAGAATGGAGAAGAAGAAATGCTTGGAGTGGAAGAAATCATGGATGAATACAAGACATTCTATTTTGCAGGAATGGAAACAACTGCAAATCTATTAACTTGGGCACTTCTACCACTAGCACAACATCAAGAATGGCAACACCGAGCTAGAGAAGAAGTCATCAAAGTTTGCGGCCACAAGACACCCCCTTCATCTAACCATTTAACTCAACTTAAACTGATGAGTATGATTATCAATGAAACACTTCTACTTTATCCTCCAGCGTTGTTGTTGGGGCCGCGGATAACAAAAGGAATGACTCTAGGAAGCATCGATGTTCCGAAAGGCACACAACTTCAAATTGTAGTGGCTGCAATCCACCACGACAAAGAGATTTGGGGAGAAGATGCAAGCGAATTCAACCCTCCGAGATTTAGTGCACCTAGGAAGCATCTGGCTTCATTTATGCCATTTGGTTTGGGTCCTAGAATTTGTGTAGGCCAACATTTGGCTTTGATTGAGGCCAAAGTTGCATTGGCTATGATAATTCAACGATTCTCTTTTACGATCTCTCCAACCTACATGCATGCCCCTGTGATGTTGGTAACCTTACAACCTCAGTTTGGTGTTCAACTCCTCATTAAGAGCTTATGGAATTGA

Protein sequence

MIKGPPYRPITGNTNNIHRLDAEAQEKAMPYIHQWSEEYGKTFVYWFGSTPRLTISDLDVIKEVLMNTNGSFTKTKASRFVKFLIGKGLAGLDGEKWALHRRIANQAFTVERVKEWLPEIRLSIYSVLEKWEEMKGGKEEFELDVRKQLRLLSADNENGEEEMLGVEEIMDEYKTFYFAGMETTANLLTWALLPLAQHQEWQHRAREEVIKVCGHKTPPSSNHLTQLKLMSMIINETLLLYPPALLLGPRITKGMTLGSIDVPKGTQLQIVVAAIHHDKEIWGEDASEFNPPRFSAPRKHLASFMPFGLGPRICVGQHLALIEAKVALAMIIQRFSFTISPTYMHAPVMLVTLQPQFGVQLLIKSLWN

Homology

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BLAST of Cla020749 vs. Swiss-Prot
Match: C734A_ARATH (Cytochrome P450 734A1 OS=Arabidopsis thaliana GN=CYP734A1 PE=2 SV=1)

HSP 1 Score: 224.6 bits (571), Expect = 1.8e-57
Identity = 115/264 (43.56%), Postives = 170/264 (64.39%), Query Frame = 1


Query: 113 VKEWL--PEIRLSIYSVLEKWEE--MKGGKEEFELDVRKQLRLLSADNENGEEEMLGVEE 172
           +K W    EIR S+  ++E+  +  + G  EE +    K L  L    +N     + V++
Sbjct: 259 LKSWKLDKEIRKSLLKLIERRRQNAIDGEGEECKEPAAKDLLGLMIQAKN-----VTVQD 318

Query: 173 IMDEYKTFYFAGMETTANLLTWALLPLAQHQEWQHRAREEVIKVCGHKTPPSSNHLTQLK 232
           I++E K+F+FAG +TT+NLLTW  + L+ H EWQ +AR+EV++VCG +  P+ +H+ +LK
Sbjct: 319 IVEECKSFFFAGKQTTSNLLTWTTILLSMHPEWQAKARDEVLRVCGSRDVPTKDHVVKLK 378

Query: 233 LMSMIINETLLLYPPALLLGPRITKGMTLGSIDVPKGTQLQIVVAAIHHDKEIWGEDASE 292
            +SMI+NE+L LYPP +    R    + LG   +P GT+L I + A+HHD+ IWG D +E
Sbjct: 379 TLSMILNESLRLYPPIVATIRRAKSDVKLGGYKIPCGTELLIPIIAVHHDQAIWGNDVNE 438

Query: 293 FNPPRFS--APR--KHLASFMPFGLGPRICVGQHLALIEAKVALAMIIQRFSFTISPTYM 352
           FNP RF+   PR  KH   F+PFGLG R C+GQ+LA+++AK+ LA++IQRF+F ++PTY 
Sbjct: 439 FNPARFADGVPRAAKHPVGFIPFGLGVRTCIGQNLAILQAKLTLAVMIQRFTFHLAPTYQ 498

Query: 353 HAPVMLVTLQPQFGVQLLIKSLWN 369
           HAP +L+ L PQ G  +  + L N
Sbjct: 499 HAPTVLMLLYPQHGAPITFRRLTN 517

HSP 2 Score: 106.7 bits (265), Expect = 5.6e-22
Identity = 57/165 (34.55%), Postives = 87/165 (52.73%), Query Frame = 1


Query: 2   IKGPPYRPITGNTNNIHRLDAEAQEKAMPY-----------IHQWSEEYGKTFVYWFGST 61
           I+GPPY    GN   +  +  +A    MP+            H W + YG TF+ WFG T
Sbjct: 47  IRGPPYHFFIGNVKELVGMMLKASSHPMPFSHNILPRVLSFYHHWRKIYGATFLVWFGPT 106

Query: 62  PRLTISDLDVIKEVLMNTNGSFTKTKASRFVKFLIGKGLAGLDGEKWALHRRIANQAFTV 121
            RLT++D D+I+E+   +   + K +A   VK L G GL  L GEKWA HR+I +  F +
Sbjct: 107 FRLTVADPDLIREIFSKSE-FYEKNEAHPLVKQLEGDGLLSLKGEKWAHHRKIISPTFHM 166

Query: 122 ERVKEWLPEIRLSIYSVLEKWEEMKGGKEEFELDVRKQLRLLSAD 156
           E +K  +P +  S+  +++KW +      E E+DV +  ++L+ D
Sbjct: 167 ENLKLLVPVVLKSVTDMVDKWSDKLSENGEVEVDVYEWFQILTED 210

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BLAST of Cla020749 vs. Swiss-Prot
Match: C7345_ORYSJ (Cytochrome P450 734A5 OS=Oryza sativa subsp. japonica GN=CYP734A5 PE=2 SV=1)

HSP 1 Score: 223.0 bits (567), Expect = 5.3e-57
Identity = 104/204 (50.98%), Postives = 142/204 (69.61%), Query Frame = 1


Query: 168 EIMDEYKTFYFAGMETTANLLTWALLPLAQHQEWQHRAREEVIKVCGHKTPPSSNHLTQL 227
           EI++E K F+FAG ET +NLLTW  + LA H EWQ RAR EV+ VCG    P+ +HL +L
Sbjct: 329 EIIEESKNFFFAGKETLSNLLTWTTVALAMHPEWQERARREVVAVCGRGDLPTKDHLPKL 388

Query: 228 KLMSMIINETLLLYPPALLLGPRITKGMTLGSIDVPKGTQLQIVVAAIHHDKEIWGEDAS 287
           K + MI+NETL LYPPA+ +     + + LG   VP GT++ I + A+HHD   WG+DA+
Sbjct: 389 KTLGMILNETLRLYPPAVAMIRTAKEDVELGGCVVPAGTEVMIPIMAVHHDAAAWGDDAA 448

Query: 288 EFNPPRFSAP-----RKHLASFMPFGLGPRICVGQHLALIEAKVALAMIIQRFSFTISPT 347
           EFNP RF+A      R+H  +FMPFG G R+C+GQ++AL+EAKVALA++++RF F +SP 
Sbjct: 449 EFNPARFAADDDGGRRRHPMAFMPFGGGARVCIGQNMALMEAKVALAVVLRRFEFRLSPA 508

Query: 348 YMHAPVMLVTLQPQFGVQLLIKSL 367
           Y+HAP +L+ L PQFG  ++ + L
Sbjct: 509 YVHAPRVLMILSPQFGAPVIFRPL 532

HSP 2 Score: 87.8 bits (216), Expect = 2.7e-16
Identity = 50/159 (31.45%), Postives = 79/159 (49.69%), Query Frame = 1


Query: 2   IKGPPYRPITGNTNNIHRLDAEAQEKAM-------------PYIHQWSEEYGKTFVYWFG 61
           ++GP YR   G++  + RL  +A  + M             P+ H W + YG   + WFG
Sbjct: 55  VRGPGYRFFVGSSIELVRLMVDAASRPMEPPTSHDILPRVLPFYHHWRKLYGPMHLIWFG 114

Query: 62  STPRLTISDLDVIKEVLMNTNGSFTKTKASRFVKFLIGKGLAGLDGEKWALHRRIANQAF 121
            TPRL +S+ ++I+EVL+     F + +A   +    G GL+ L GE+WA  RR+   AF
Sbjct: 115 RTPRLVVSEPELIREVLLTRADHFDRYEAHPMICQFEGYGLSNLHGERWARRRRVLTPAF 174

Query: 122 TVERVKEWLPEIRLSIYSVLEKWEE--MKGGKEEFELDV 146
             E ++   P +  ++  +L++  E    GG  E E+DV
Sbjct: 175 HTENLRMIAPFVAGTVTRMLDELAERARAGGAGEAEVDV 213

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BLAST of Cla020749 vs. Swiss-Prot
Match: C7A29_PANGI (Cytochrome P450 CYP72A219 OS=Panax ginseng PE=2 SV=1)

HSP 1 Score: 219.5 bits (558), Expect = 5.9e-56
Identity = 115/267 (43.07%), Postives = 172/267 (64.42%), Query Frame = 1


Query: 111 ERVKEWLPEIRLSIYSVLEK-WEEMKGGKEEFELDVRKQLRLLSADN-----ENGEEEM- 170
           +R+KE   E+++++ S++ K  + M+ G+     D+   L +L   N     ++G     
Sbjct: 255 KRMKEIAKEVKIALKSIINKRLKAMEAGERSSHDDL---LGILLESNSKEIKQHGNTNFG 314

Query: 171 LGVEEIMDEYKTFYFAGMETTANLLTWALLPLAQHQEWQHRAREEVIKVCGHKTPPSSNH 230
           L V+E+++E K F+FAG ETT+NLL W ++ L+QHQ+WQ RA+EEV++  G+  P     
Sbjct: 315 LTVDEVIEECKLFFFAGQETTSNLLVWTMILLSQHQDWQKRAKEEVLRTFGNNKPDFDG- 374

Query: 231 LTQLKLMSMIINETLLLYPPALLLGPRITKGMTLGSIDVPKGTQLQIVVAAIHHDKEIWG 290
           L  LK+++MI+ E L LYPP L L   I + + LG I +P G  L + +  +H+D+EIWG
Sbjct: 375 LNHLKVVNMILLEVLRLYPPILSLDRTIYEEIKLGEISLPAGVILLLPIILLHYDQEIWG 434

Query: 291 EDASEFNPPRFSA----PRKHLASFMPFGLGPRICVGQHLALIEAKVALAMIIQRFSFTI 350
           +DA EFNP RFS       K   ++ PF  GPRIC+GQ+ A++EAK+A+AMI+QRFSF +
Sbjct: 435 DDAKEFNPERFSEGVLKATKGRVTYFPFSWGPRICIGQNFAMLEAKMAMAMILQRFSFVL 494

Query: 351 SPTYMHAPVMLVTLQPQFGVQLLIKSL 367
           SP+Y HAP  ++TLQPQ+G  L++ SL
Sbjct: 495 SPSYAHAPHAIITLQPQYGAHLILHSL 517

HSP 2 Score: 85.1 bits (209), Expect = 1.7e-15
Identity = 47/117 (40.17%), Postives = 65/117 (55.56%), Query Frame = 1


Query: 39  YGKTFVYWFGSTPRLTISDLDVIKEVLMNTNGSFTKTKASRFVKFLIGKGLAGLDGEKWA 98
           YGK    W G  P + I + D IK+VL      F K + +   K L+  G+A  +G++WA
Sbjct: 92  YGKNSFLWLGPKPMVHIMNPDHIKDVLSKFY-QFQKPRHNPLTK-LLATGVADAEGDRWA 151

Query: 99  LHRRIANQAFTVERVKEWLPEIRLSIYSVLEKWEEMKGGKEEFELDVRKQLRLLSAD 156
            HR++ N AF +E++K  LP I LS   ++ KWEEM   K +FELDV   L  L++D
Sbjct: 152 KHRKLINPAFHLEKLKNMLPAIYLSSSEIVTKWEEMVSTKGQFELDVLPYLETLTSD 206

[Close]

BLAST of Cla020749 vs. Swiss-Prot
Match: C7A15_ARATH (Cytochrome P450 72A15 OS=Arabidopsis thaliana GN=CYP72A15 PE=2 SV=1)

HSP 1 Score: 218.0 bits (554), Expect = 1.7e-55
Identity = 108/220 (49.09%), Postives = 146/220 (66.36%), Query Frame = 1


Query: 151 LLSADNENGEEEMLGVEEIMDEYKTFYFAGMETTANLLTWALLPLAQHQEWQHRAREEVI 210
           LL ++    E   +  E++M+E K FYFAG ETT+ LL W ++ L+QHQ+WQ RAREEV 
Sbjct: 294 LLESNLRQTEGNGMSTEDLMEECKLFYFAGQETTSVLLVWTMVLLSQHQDWQARAREEVK 353

Query: 211 KVCGHKTPPSSNHLTQLKLMSMIINETLLLYPPALLLGPRITKGMTLGSIDVPKGTQLQI 270
           +V G K P +   L QLK+M+MI+ E L LYPP   L   I K + LG + +P G Q+ +
Sbjct: 354 QVFGDKEPDAEG-LNQLKVMTMILYEVLRLYPPVTQLTRAIHKELKLGDLTLPGGVQISL 413

Query: 271 VVAAIHHDKEIWGEDASEFNPPRF----SAPRKHLASFMPFGLGPRICVGQHLALIEAKV 330
            +  + HD E+WG DA+EFNP RF    S   K   SF PF  GPRIC+GQ+ AL+EAK+
Sbjct: 414 PILLVQHDIELWGNDAAEFNPDRFKDGLSKATKSQVSFFPFAWGPRICIGQNFALLEAKM 473

Query: 331 ALAMIIQRFSFTISPTYMHAPVMLVTLQPQFGVQLLIKSL 367
           A+A+I++RFSF ISP+Y+HAP  ++T+ PQFG QL++  L
Sbjct: 474 AMALILRRFSFEISPSYVHAPYTVITIHPQFGAQLIMHKL 512

HSP 2 Score: 94.7 bits (234), Expect = 2.2e-18
Identity = 58/167 (34.73%), Postives = 84/167 (50.30%), Query Frame = 1


Query: 2   IKGPPYRPITGNTNNIHRLDAEAQEKAM-----------PYIHQWSEEYGKTFVYWFGST 61
           + G PY P+ G+      + +EA+ K +           PY  Q  + YG+T+  WFG  
Sbjct: 44  LAGTPYTPLVGDLKKNFTMLSEARSKPLKLTDDISPRVVPYPLQMFKTYGRTYFTWFGPI 103

Query: 62  PRLTISDLDVIKEVLMNTNGSFTKTKASRF-VKFLIGKGLAGLDGEKWALHRRIANQAFT 121
           P +TI D + IKEV    N  +   K   F +  +I KGLA  DG+KWA HRRI N AF 
Sbjct: 104 PTITIMDPEQIKEVF---NKVYDFQKPHTFPLATIIAKGLANYDGDKWAKHRRIINPAFH 163

Query: 122 VERVKEWLPEIRLSIYSVLEKWEEMKGGK-EEFELDVRKQLRLLSAD 156
           +E++K  +P    S   V+ +W+++   K    E+DV   L  ++AD
Sbjct: 164 IEKIKNMVPAFHQSCREVVGEWDQLVSDKGSSCEVDVWPGLVSMTAD 207

[Close]

BLAST of Cla020749 vs. Swiss-Prot
Match: C7344_ORYSJ (Cytochrome P450 734A4 OS=Oryza sativa subsp. japonica GN=CYP734A4 PE=2 SV=1)

HSP 1 Score: 215.3 bits (547), Expect = 1.1e-54
Identity = 112/266 (42.11%), Postives = 163/266 (61.28%), Query Frame = 1


Query: 119 EIRLSIYSVLEKWEEMKGGKEEFELDVRKQLR------LLSADNENGEEEMLGVEEIMDE 178
           EIR  +  ++ +     GG+E+      K  +      LL      G +  + VE++++E
Sbjct: 265 EIRRGLVRLIGRRSGGDGGEEDETTTELKDKQDSGFNDLLGLMINAGVDRTMPVEDMVEE 324

Query: 179 YKTFYFAGMETTANLLTWALLPLAQHQEWQHRAREEVIKVCGHKTP--PSSNHLTQLKLM 238
            KTF+FAG +TT NLLTWA + LA H +WQ RAR EV+ VCG      P+ +HL +LK +
Sbjct: 325 CKTFFFAGKQTTTNLLTWATVLLAMHPDWQDRARREVLAVCGDAAGELPTKDHLPKLKTL 384

Query: 239 SMIINETLLLYPPALLLGPRITKGMTLGS--------IDVPKGTQLQIVVAAIHHDKEIW 298
            MI+NETL LYPPA+    R    +TLG         I +P+ T+L + + AIHHD  +W
Sbjct: 385 GMILNETLRLYPPAVATIRRAKFDVTLGGGGDGDAGGIHIPRDTELLVPIMAIHHDARLW 444

Query: 299 GEDASEFNPPRFSA----PRKHLASFMPFGLGPRICVGQHLALIEAKVALAMIIQRFSFT 358
           G DA++FNP RF++      KH  +F+PFGLG R+C+GQ LA++EAK+ +A+++QRF   
Sbjct: 445 GPDAAQFNPARFASGAARAAKHPLAFIPFGLGSRMCIGQSLAILEAKLTMAVLLQRFDLA 504

Query: 359 ISPTYMHAPVMLVTLQPQFGVQLLIK 365
           +SPTY+HAP +L+ L PQ+G  L+ +
Sbjct: 505 LSPTYVHAPTVLMLLHPQYGAPLIFR 530

HSP 2 Score: 106.3 bits (264), Expect = 7.3e-22
Identity = 54/168 (32.14%), Postives = 88/168 (52.38%), Query Frame = 1


Query: 2   IKGPPYRPITGNTNNIHRLDAEAQEKAMP-------------YIHQWSEEYGKTFVYWFG 61
           ++GPPYR + G    +  L AEA  K MP             + H W + YG TF+ WFG
Sbjct: 41  VRGPPYRFLVGCVREMVALMAEATAKPMPPAAPHNALPRVLAFYHYWRKIYGPTFLIWFG 100

Query: 62  STPRLTISDLDVIKEVLMNTNGSFTKTKASRFVKFLIGKGLAGLDGEKWALHRRIANQAF 121
            TPRLT+++ ++++E+ +    +F + +A   V+ L G GL  L G+KWA HRR+    F
Sbjct: 101 PTPRLTVAEPEMVREIFLTRAEAFDRYEAHPVVRQLEGDGLVSLHGDKWAHHRRVLTPGF 160

Query: 122 TVERVKEWLPEIRLSIYSVLEKWEEMK-GGKEEFELDVRKQLRLLSAD 156
             + +   +P +  S+ ++ E+W  M   G  E E+DV +  + ++ +
Sbjct: 161 YPDNLNRLVPHVGRSVAALAERWRAMACAGGGEVEVDVAEWFQAVAEE 208

[Close]

BLAST of Cla020749 vs. TrEMBL
Match: A0A067H0N4_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g012459mg PE=3 SV=1)

HSP 1 Score: 407.5 bits (1046), Expect = 1.7e-110
Identity = 208/418 (49.76%), Postives = 278/418 (66.51%), Query Frame = 1


Query: 2   IKGPPYRPITGNTNNIHRLDAEAQEKAM------------PYIHQWSEEYGKTFVYWFGS 61
           + GP YR ITGN+  I RL  EAQ K +            P  H+WS  YGKTF+YWFGS
Sbjct: 46  VGGPGYRLITGNSAEIRRLFIEAQSKPISPIHHDILHRTAPLYHEWSRMYGKTFLYWFGS 105

Query: 62  TPRLTISDLDVIKEVLMNTNGSFTKTKASRFVKFLIGKGLAGLDGEKWALHRRIANQAFT 121
            PRL ISD D+IKEVLM+T+GSF K   +   + L G+GL GL G+KWA+HRRI+NQAF 
Sbjct: 106 KPRLAISDPDMIKEVLMDTSGSFEKIGFNPLSRQLFGQGLVGLTGDKWAIHRRISNQAFN 165

Query: 122 VERVKEWLPEIRLSIYSVLEKWEEMKGG-------------------KEEFELDVRKQLR 181
           +ERVK W+P+I  S    L   ++++ G                   ++  E + R+ +R
Sbjct: 166 MERVKGWVPDIVASPRRCLRIGKKLEEGEMNLKLMCIKFLPTKKNRERQRLESETRESIR 225

Query: 182 LL--------------------SADNENGEEEMLGVEEIMDEYKTFYFAGMETTANLLTW 241
           +L                    S  N +G+EE L VEEI+DE KTFYFAG ETTANLLTW
Sbjct: 226 MLIKNNGKTRESSRNLLSLLMSSCKNHDGQEERLDVEEIIDECKTFYFAGKETTANLLTW 285

Query: 242 ALLPLAQHQEWQHRAREEVIKVCGHKTPPSSNHLTQLKLMSMIINETLLLYPPALLLGPR 301
           AL+ LA HQEWQ +AREEV +VCG      +  L+ LK+++MI+NETL LYPPA++L  +
Sbjct: 286 ALVLLAMHQEWQIKAREEVFQVCGDNDLRLAEKLSDLKIVNMILNETLRLYPPAVMLMRQ 345

Query: 302 ITKGMTLGSIDVPKGTQLQIVVAAIHHDKEIWGEDASEFNPPRFSAPRKHLASFMPFGLG 361
            ++G+ LG++DVP GTQL I + A+HHD ++WGEDA+EFNP RF+  RKHLASF PFGLG
Sbjct: 346 ASRGVKLGNLDVPAGTQLYIALTAVHHDTDLWGEDANEFNPLRFNESRKHLASFFPFGLG 405

Query: 362 PRICVGQHLALIEAKVALAMIIQRFSFTISPTYMHAPVMLVTLQPQFGVQLLIKSLWN 369
           PR+CVGQ+LA++EAKV LA+II+RF+F +SP+Y+HAP++ +++QPQ G  +L   + N
Sbjct: 406 PRVCVGQNLAMVEAKVVLAIIIRRFNFFLSPSYVHAPMLFISMQPQHGAHILFSRIHN 463

[Close]

BLAST of Cla020749 vs. TrEMBL
Match: A0A0A0LD75_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G855340 PE=3 SV=1)

HSP 1 Score: 317.4 bits (812), Expect = 2.3e-83
Identity = 150/216 (69.44%), Postives = 183/216 (84.72%), Query Frame = 1


Query: 153 SADNENGEEEMLGVEEIMDEYKTFYFAGMETTANLLTWALLPLAQHQEWQHRAREEVIKV 212
           S  N+NGEEE LG+E+I+DE KTFYFAGMETTA+LLTWALL LA+HQEWQ +AREEV+KV
Sbjct: 286 SYKNQNGEEERLGIEDIIDECKTFYFAGMETTAHLLTWALLLLAKHQEWQDKAREEVLKV 345

Query: 213 CGHKTPPSSNHLTQLKLMSMIINETLLLYPPALLLGPRITKGMTLGSIDVPKGTQLQIVV 272
           CG+K PP++ +L +LKL+ MIINETL LY PA++L    +K +TLGS+D+P GT+L + +
Sbjct: 346 CGYKKPPAAENLNELKLVGMIINETLRLYSPAVMLTRTASKQLTLGSLDIPAGTELFLAL 405

Query: 273 AAIHHDKEIWGEDASEFNPPRFSAPRKHLASFMPFGLGPRICVGQHLALIEAKVALAMII 332
           AA+HHDKE WGEDA+ FNP RF  PRKHLASF+PF LGPRICVGQ+LALIE KVALAMII
Sbjct: 406 AAVHHDKEFWGEDANCFNPLRFCEPRKHLASFLPFSLGPRICVGQNLALIETKVALAMII 465

Query: 333 QRFSFTISPTYMHAPVMLVTLQPQFGVQLLIKSLWN 369
           QRFSF +SPTY H+P++ +TLQPQFG QLL +SL N
Sbjct: 466 QRFSFAVSPTYTHSPMLFITLQPQFGAQLLFRSLRN 501

[Close]

BLAST of Cla020749 vs. TrEMBL
Match: A0A0A0LD75_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G855340 PE=3 SV=1)

HSP 1 Score: 203.0 bits (515), Expect = 6.3e-49
Identity = 115/249 (46.18%), Postives = 148/249 (59.44%), Query Frame = 1


Query: 2   IKGPPYRPITGNTNNIHRLDAEAQEKAMPYIH-----------QWSEEYGKTFVYWFGST 61
           I GPPYRP  GN+ +I RL  EAQ   +P+ H           QWS +YGKTF+YWFGS 
Sbjct: 36  ITGPPYRPFIGNSAHIRRLFKEAQSNPIPFHHDILSRVLPFYFQWSRQYGKTFLYWFGSK 95

Query: 62  PRLTISDLDVIKEVLMNTNGSFTKTKASRFVKFLIGKGLAGLDGEKWALHRRIANQAFTV 121
           PRL ISD D+IKEVL+NT G F +   +   K L G+GL GL+GEKW   R+IANQAF +
Sbjct: 96  PRLAISDPDLIKEVLVNTRGYFRRIHFNPLSKVLFGEGLIGLEGEKWVARRKIANQAFNI 155

Query: 122 ERVKEWLPEIRLSIYSVLEKWEEMKGGKEEFELDVRKQLRLLSADNENGEEEMLGVEE-- 181
           ERVK W+PEI +S+ SVLEKWEEMKGG EEFELDV K++R LSAD  +        EE  
Sbjct: 156 ERVKGWVPEIVVSVLSVLEKWEEMKGGMEEFELDVHKEVRRLSADVISRTAFGSNFEEGK 215

Query: 182 ---IMDEYKTFYFAGMETTANLLTWALLPLAQHQE-WQ-HRAREEVIKVCGHKTPPSSNH 233
               + E +T+ F+    +  +  +  LP  +++E W   +   E+IKV          +
Sbjct: 216 RIFSLQEQQTYLFSQAIRSVYIPGFRFLPTKKNRERWSLEKETRELIKVLIETNSKGREN 275

HSP 2 Score: 302.0 bits (772), Expect = 1.0e-78
Identity = 163/410 (39.76%), Postives = 250/410 (60.98%), Query Frame = 1


Query: 2   IKGPPYRPITGNTNNIHRLDAEAQEKAMPY-----------IHQWSEEYGKTFVYWFGST 61
           I+GP Y PI GN+  I ++  EA+ K++P+            +QWS +YGKTF++WFG  
Sbjct: 470 IEGPGYCPIFGNSREIMKMTIEAESKSIPFNQDILHRIAPHYYQWSAKYGKTFLFWFGMK 529

Query: 62  PRLTISDLDVIKEVLMNTNG-SFTKTKASRFVKFLIGKGLAGLDGEKWALHRRIANQAFT 121
           PRL ++D +++K +L N++  +F K   +     LIG+GL GL GEKW LHR+IA+ AF 
Sbjct: 530 PRLAVADPEMVKMILPNSSSDTFDKIGFNPLAGVLIGQGLNGLVGEKWTLHRKIASPAFN 589

Query: 122 VERVKEWLPEIRLSIYSVLEKWEEMKGGKEEFELDVRKQLRLL----------SADNENG 181
           +ERVK W+PE+  S+  ++EKWEE   GK+  EL+++ +   L          S+  E G
Sbjct: 590 MERVKAWVPEMVASVVKMMEKWEEQSRGKDHCELEMQNEFHDLIAEILSKTAFSSSFEEG 649

Query: 182 ------EEEMLGVEEIMDEYKTFYFAG---METTANLLTWALLPLAQHQEWQHRAREEVI 241
                 +E+ +G+  +    ++ YF G   + T  N L W+L         + + R+ + 
Sbjct: 650 KHIFQLQEQQVGLALL--ALRSVYFPGFRFLPTKTNRLRWSL---------EKQTRDSIR 709

Query: 242 KV--CGHKTPPSSNHL---------TQLKLMSMIINETLLLYPPALLLGPRITKGMTLGS 301
           K+     KT  +S +L          +L  +  I++ETL LYPP   +  + TK + +G 
Sbjct: 710 KIIRTNEKTRDNSKNLLNQLMSANMNELGQVGWILDETLRLYPPIANMTRKTTKKVVVGG 769

Query: 302 IDVPKGTQLQIVVAAIHHDKEIWGEDASEFNPPRFSAP-RKHLASFMPFGLGPRICVGQH 361
           +++P GT+  + VAAIHHD+EIWG DA EFNP RFS    +HLAS+MPFGLG RIC+GQ+
Sbjct: 770 LNIPAGTEFYLAVAAIHHDEEIWGADAKEFNPSRFSGTCSRHLASYMPFGLGSRICIGQN 829

Query: 362 LALIEAKVALAMIIQRFSFTISPTYMHAPVMLVTLQPQFGVQLLIKSLWN 369
           LA++EAK+ LA I+++F+F +SP+Y+HAPVM  T+QPQ+G  +L++ + N
Sbjct: 830 LAVVEAKIILATILKKFAFVVSPSYVHAPVMSFTVQPQYGAHVLVQKILN 868

[Close]

BLAST of Cla020749 vs. TrEMBL
Match: A0A164ZR13_DAUCA (Uncharacterized protein OS=Daucus carota subsp. sativus GN=DCAR_019516 PE=4 SV=1)

HSP 1 Score: 131.7 bits (330), Expect = 1.8e-27
Identity = 79/220 (35.91%), Postives = 113/220 (51.36%), Query Frame = 1


Query: 2   IKGPPYRPITGNTNNIHRLDAEAQEKAM------------PYIHQWSEEYGKTFVYWFGS 61
           + GP YRP  GNT  +  +  EA  K++            P  +QWS +YGKTF+ WFG 
Sbjct: 37  VNGPNYRPFYGNTAEMTHMTKEALSKSISSLNHDILHRVSPDYYQWSAKYGKTFLCWFGM 96

Query: 62  TPRLTISDLDVIKEVLMNTNGSFTKTKASRFVKFLIGKGLAGLDGEKWALHRRIANQAFT 121
            PRL + D D+IK+V++ T  +  +   +   + L  +GL GL G KWA HR+I   AF 
Sbjct: 97  RPRLVLVDPDMIKDVMLKTTDTNDRDDYNPLSRLLFAQGLPGLMGHKWAAHRKIEAPAFN 156

Query: 122 VERVKEWLPEIRLSIYSVLEKWEEMKGGKEEFELDVRKQLRLLSA----------DNENG 181
           V+R+K W+P I  ++  +L  WE+  G K EFE+DV +++  LSA          D E G
Sbjct: 157 VDRIKAWVPGIVATVTIMLNNWEQKIGEKIEFEVDVHQEIHNLSAEVISEIAIGSDFEEG 216

Query: 182 EE--EMLGVEEIM--DEYKTFYFAG---METTANLLTWAL 193
               E+L  +EI+        Y  G   + T  N L W L
Sbjct: 217 RRIFEILQQQEILAHQAMHNVYIPGFRFLPTKMNKLRWRL 256

HSP 2 Score: 112.1 bits (279), Expect = 1.5e-21
Identity = 54/122 (44.26%), Postives = 80/122 (65.57%), Query Frame = 1


Query: 150 RLLSADNENGEEEMLGVEEIMDEYKTFYFAGMETTANLLTWALLPLAQHQEWQHRAREEV 209
           RL+   N N     L +EEI+DE KT ++ G E  AN L WALL LAQHQEWQ++AREEV
Sbjct: 284 RLMPGSNMNKHGPGLEIEEIIDECKTIFYGGKEAVANALAWALLLLAQHQEWQNKAREEV 343

Query: 210 IKVCGHKTPPSSNHLTQLKLMSMIINETLLLYPPALLLGPRITKGMTLGSIDVPKGTQLQ 269
           ++VC      ++ +L +LK++ +II ETL LYP    +  R  K + +GS+++P GT++ 
Sbjct: 344 LRVCKGNEHLAAENLQELKIVGLIIKETLRLYPVDNSISRRTLKNVKVGSLNIPAGTEVS 403

Query: 270 IV 272
           ++
Sbjct: 404 LL 405

HSP 3 Score: 294.7 bits (753), Expect = 1.6e-76
Identity = 141/237 (59.49%), Postives = 181/237 (76.37%), Query Frame = 1


Query: 130 KWEEMKGGKEEFELDVRKQLRLLSADNENGEEEMLGVEEIMDEYKTFYFAGMETTANLLT 189
           K  E K  + E   +V   L + S  N+ GEEE LG+EEI+DE KT YFAG ETTANLLT
Sbjct: 267 KLVETKNNERENARNVLSSL-MCSYKNDAGEEEKLGLEEIIDECKTIYFAGKETTANLLT 326

Query: 190 WALLPLAQHQEWQHRAREEVIKVCGHKTPPSSNHLTQLKLMSMIINETLLLYPPALLLGP 249
           WALL LA+HQEWQ +AREEV++  GH  PP +++L  LK +SMIINETL LYPPA++L  
Sbjct: 327 WALLLLAKHQEWQSKAREEVLRFVGHNRPPVADNLNDLKTVSMIINETLRLYPPAVMLMR 386

Query: 250 RITKGMTLGSIDVPKGTQLQIVVAAIHHDKEIWGEDASEFNPPRFSAPRKHLASFMPFGL 309
           + +K + LGSI+VP  TQL + + A+HHD+EIWGED   FNP RFS PRKHLA+F PFGL
Sbjct: 387 QTSKDVVLGSINVPAKTQLYLALTAVHHDREIWGEDCHSFNPMRFSEPRKHLAAFFPFGL 446

Query: 310 GPRICVGQHLALIEAKVALAMIIQRFSFTISPTYMHAPVMLVTLQPQFGVQLLIKSL 367
           GPR CVGQ+LA++EAK+ALAMIIQ +SF +SP Y+HAP++ +TLQPQ+G Q++ + +
Sbjct: 447 GPRTCVGQNLAMVEAKIALAMIIQSYSFVVSPNYVHAPILFITLQPQYGAQIVFRRI 502

[Close]

BLAST of Cla020749 vs. TrEMBL
Match: A0A151QX44_CAJCA (Secologanin synthase OS=Cajanus cajan GN=KK1_044052 PE=3 SV=1)

HSP 1 Score: 166.4 bits (420), Expect = 6.6e-38
Identity = 81/166 (48.80%), Postives = 111/166 (66.87%), Query Frame = 1


Query: 2   IKGPPYRPITGNTNNIHRLDAEAQE------------KAMPYIHQWSEEYGKTFVYWFGS 61
           I GP YRPI GN++ I RL AEA+             + +P+  +WS  YGKTF+YWFGS
Sbjct: 38  IGGPGYRPILGNSSEIRRLYAEAKSEPTHRFDHDVLRRVVPFYDRWSGAYGKTFLYWFGS 97

Query: 62  TPRLTISDLDVIKEVLMNTNGSFTKTKASRFVKFLIGKGLAGLDGEKWALHRRIANQAFT 121
           TPRL I++ D+IKEVL N +G + +   +   K L G+GL GL G +WALHRRI N AF 
Sbjct: 98  TPRLAITEPDMIKEVLSNKSGEYERVSFNPQSKLLFGQGLVGLQGPQWALHRRIINLAFN 157

Query: 122 VERVKEWLPEIRLSIYSVLEKWEEMKGGKEEFELDVRKQLRLLSAD 156
           +E VK W+P+I  S+  ++E+WEE +GGK+E E++V ++L  LSAD
Sbjct: 158 MELVKGWVPDIVASVTKMVERWEEQRGGKDEVEINVHRELLHLSAD 203

HSP 2 Score: 287.3 bits (734), Expect = 2.6e-74
Identity = 143/248 (57.66%), Postives = 185/248 (74.60%), Query Frame = 1


Query: 119 EIRLSIYSVLEKWEEMKGGKEEFELDVRKQLRLLSADNENGEEEMLGVEEIMDEYKTFYF 178
           E R SI  ++E     K    E   +V   L + S  N+ G EE LGVEEI+DE KT YF
Sbjct: 263 ETRESILKLIET----KSNTRENARNVLSSL-MCSYKNDAGGEEKLGVEEIIDECKTIYF 322

Query: 179 AGMETTANLLTWALLPLAQHQEWQHRAREEVIKVCGHKTPPSSNHLTQLKLMSMIINETL 238
           AG ETTANLLTWALL LA+HQEWQ +AR+EV+ V G    P++++L  LK+++MIINETL
Sbjct: 323 AGKETTANLLTWALLLLAKHQEWQSKARKEVLHVIGRNRLPAADNLNDLKIVTMIINETL 382

Query: 239 LLYPPALLLGPRITKGMTLGSIDVPKGTQLQIVVAAIHHDKEIWGEDASEFNPPRFSAPR 298
            LYPPA++L  + +K + LGSI++P  TQL + + A+HHD+EIWGED   FNP RFS PR
Sbjct: 383 RLYPPAVMLMRQASKDVMLGSINIPAKTQLFLALTAVHHDREIWGEDYHNFNPMRFSEPR 442

Query: 299 KHLASFMPFGLGPRICVGQHLALIEAKVALAMIIQRFSFTISPTYMHAPVMLVTLQPQFG 358
           KHLA+F PFGLGPRICVGQ+LAL+EAK+ALA+IIQ +SF +SP YMHAP++ VTLQPQ+G
Sbjct: 443 KHLAAFFPFGLGPRICVGQNLALVEAKIALALIIQSYSFVLSPNYMHAPILFVTLQPQYG 502

Query: 359 VQLLIKSL 367
            Q++ + +
Sbjct: 503 AQIIFRKI 505

[Close]

BLAST of Cla020749 vs. NCBI nr
Match: gi|641865795|gb|KDO84480.1| (hypothetical protein CISIN_1g012459mg [Citrus sinensis])

HSP 1 Score: 407.5 bits (1046), Expect = 2.4e-110
Identity = 208/418 (49.76%), Postives = 278/418 (66.51%), Query Frame = 1


Query: 2   IKGPPYRPITGNTNNIHRLDAEAQEKAM------------PYIHQWSEEYGKTFVYWFGS 61
           + GP YR ITGN+  I RL  EAQ K +            P  H+WS  YGKTF+YWFGS
Sbjct: 46  VGGPGYRLITGNSAEIRRLFIEAQSKPISPIHHDILHRTAPLYHEWSRMYGKTFLYWFGS 105

Query: 62  TPRLTISDLDVIKEVLMNTNGSFTKTKASRFVKFLIGKGLAGLDGEKWALHRRIANQAFT 121
            PRL ISD D+IKEVLM+T+GSF K   +   + L G+GL GL G+KWA+HRRI+NQAF 
Sbjct: 106 KPRLAISDPDMIKEVLMDTSGSFEKIGFNPLSRQLFGQGLVGLTGDKWAIHRRISNQAFN 165

Query: 122 VERVKEWLPEIRLSIYSVLEKWEEMKGG-------------------KEEFELDVRKQLR 181
           +ERVK W+P+I  S    L   ++++ G                   ++  E + R+ +R
Sbjct: 166 MERVKGWVPDIVASPRRCLRIGKKLEEGEMNLKLMCIKFLPTKKNRERQRLESETRESIR 225

Query: 182 LL--------------------SADNENGEEEMLGVEEIMDEYKTFYFAGMETTANLLTW 241
           +L                    S  N +G+EE L VEEI+DE KTFYFAG ETTANLLTW
Sbjct: 226 MLIKNNGKTRESSRNLLSLLMSSCKNHDGQEERLDVEEIIDECKTFYFAGKETTANLLTW 285

Query: 242 ALLPLAQHQEWQHRAREEVIKVCGHKTPPSSNHLTQLKLMSMIINETLLLYPPALLLGPR 301
           AL+ LA HQEWQ +AREEV +VCG      +  L+ LK+++MI+NETL LYPPA++L  +
Sbjct: 286 ALVLLAMHQEWQIKAREEVFQVCGDNDLRLAEKLSDLKIVNMILNETLRLYPPAVMLMRQ 345

Query: 302 ITKGMTLGSIDVPKGTQLQIVVAAIHHDKEIWGEDASEFNPPRFSAPRKHLASFMPFGLG 361
            ++G+ LG++DVP GTQL I + A+HHD ++WGEDA+EFNP RF+  RKHLASF PFGLG
Sbjct: 346 ASRGVKLGNLDVPAGTQLYIALTAVHHDTDLWGEDANEFNPLRFNESRKHLASFFPFGLG 405

Query: 362 PRICVGQHLALIEAKVALAMIIQRFSFTISPTYMHAPVMLVTLQPQFGVQLLIKSLWN 369
           PR+CVGQ+LA++EAKV LA+II+RF+F +SP+Y+HAP++ +++QPQ G  +L   + N
Sbjct: 406 PRVCVGQNLAMVEAKVVLAIIIRRFNFFLSPSYVHAPMLFISMQPQHGAHILFSRIHN 463

[Close]

BLAST of Cla020749 vs. NCBI nr
Match: gi|778688995|ref|XP_004147813.2| (PREDICTED: cytochrome P450 734A6-like [Cucumis sativus])

HSP 1 Score: 352.1 bits (902), Expect = 1.2e-93
Identity = 176/250 (70.40%), Postives = 205/250 (82.00%), Query Frame = 1


Query: 119 EIRLSIYSVLEKWEEMKGGKEEFELDVRKQLRLLSADNENGEEEMLGVEEIMDEYKTFYF 178
           E R SI +++E     K  KE+        L L S  N+NGE E LGV+E+++E KTFYF
Sbjct: 260 ETRASIKALVES---EKNNKEKENSSNLLSLLLSSYKNQNGEIEKLGVDEVVNECKTFYF 319

Query: 179 AGMETTANLLTWALLPLAQHQEWQHRAREEVIKVCGHKTPPSSNHLTQLKLMSMIINETL 238
           AGMETTANLLTWALL LA+HQEWQ RAREEVI VCG KTPP+S++LT+LKL+ MIINETL
Sbjct: 320 AGMETTANLLTWALLLLAEHQEWQDRAREEVINVCGQKTPPTSDNLTELKLVGMIINETL 379

Query: 239 LLYPPALLLGPRITKGMTLGSIDVPKGTQLQIVVAAIHHDKEIWGEDASEFNPPRFSAPR 298
            LYPPA ++  R  K +TLG+IDVP+GTQLQ+ V AIHHDKE+WGEDAS FNP RFS PR
Sbjct: 380 RLYPPATMMMRRTMKRLTLGNIDVPEGTQLQLSVVAIHHDKELWGEDASNFNPMRFSEPR 439

Query: 299 KHLASFMPFGLGPRICVGQHLALIEAKVALAMIIQRFSFTISPTYMHAPVMLVTLQPQFG 358
           KHLASF+PFGLGPRICVGQHLALIEAKVALAMIIQ+FSFT+SPTY HAP+M V+L PQFG
Sbjct: 440 KHLASFLPFGLGPRICVGQHLALIEAKVALAMIIQQFSFTVSPTYTHAPMMFVSLNPQFG 499

Query: 359 VQLLIKSLWN 369
           VQLL++SLWN
Sbjct: 500 VQLLVRSLWN 506

[Close]

BLAST of Cla020749 vs. NCBI nr
Match: gi|778688995|ref|XP_004147813.2| (PREDICTED: cytochrome P450 734A6-like [Cucumis sativus])

HSP 1 Score: 195.3 bits (495), Expect = 1.9e-46
Identity = 98/165 (59.39%), Postives = 115/165 (69.70%), Query Frame = 1


Query: 2   IKGPPYRPITGNTNNIHRLDAEAQEKA-----------MPYIHQWSEEYGKTFVYWFGST 61
           I GP Y PI GN  ++ R+  EAQ K            +PYIHQWS EYGK FVYWFG  
Sbjct: 39  ITGPRYCPIIGNATDMRRMYMEAQAKTIPLTHDILCRVLPYIHQWSTEYGKMFVYWFGPK 98

Query: 62  PRLTISDLDVIKEVLMNTNGSFTKTKASRFVKFLIGKGLAGLDGEKWALHRRIANQAFTV 121
           PRL ISD  +IKEVL NT G F K   +   K L G+GL GL+ ++W +HRRIANQAFT+
Sbjct: 99  PRLAISDPVMIKEVLTNTGGPFRKVGFTPVSKLLFGEGLVGLEDKQWVIHRRIANQAFTI 158

Query: 122 ERVKEWLPEIRLSIYSVLEKWEEMKGGKEEFELDVRKQLRLLSAD 156
           +RVK WLPEI LS+ +VLEKWEEMK G EEFE+DV KQLRLL+AD
Sbjct: 159 DRVKGWLPEITLSVRNVLEKWEEMKEGMEEFEVDVHKQLRLLTAD 203

HSP 2 Score: 344.4 bits (882), Expect = 2.5e-91
Identity = 170/250 (68.00%), Postives = 204/250 (81.60%), Query Frame = 1


Query: 119 EIRLSIYSVLEKWEEMKGGKEEFELDVRKQLRLLSADNENGEEEMLGVEEIMDEYKTFYF 178
           E R SI +++E  +  K  +    L    +L L S  N+NGE E LGV+E+++E KTFYF
Sbjct: 259 ETRASIKALVESEKNRKERENSTNL---LRLLLSSYKNQNGEIETLGVDEVVNECKTFYF 318

Query: 179 AGMETTANLLTWALLPLAQHQEWQHRAREEVIKVCGHKTPPSSNHLTQLKLMSMIINETL 238
           AGMETTANLLTWALL LA+HQEWQ R REEVI VCG KTPP++++LT+LKL+ MI+NETL
Sbjct: 319 AGMETTANLLTWALLLLAEHQEWQDRVREEVINVCGQKTPPTTDNLTELKLVGMIVNETL 378

Query: 239 LLYPPALLLGPRITKGMTLGSIDVPKGTQLQIVVAAIHHDKEIWGEDASEFNPPRFSAPR 298
            LYPPA+++  R  K +TLG+IDVP+GTQLQ+ V AIHHD E+WGEDA  FNP RFS  R
Sbjct: 379 RLYPPAIMMMRRTMKRLTLGNIDVPEGTQLQLSVVAIHHDTELWGEDAHTFNPMRFSESR 438

Query: 299 KHLASFMPFGLGPRICVGQHLALIEAKVALAMIIQRFSFTISPTYMHAPVMLVTLQPQFG 358
           KHLASF+PFGLGPRICVGQHLALIEAKVALAMIIQRF+FT+SPTYMHAP+M V+L PQFG
Sbjct: 439 KHLASFLPFGLGPRICVGQHLALIEAKVALAMIIQRFAFTVSPTYMHAPMMFVSLNPQFG 498

Query: 359 VQLLIKSLWN 369
           VQLL++SLWN
Sbjct: 499 VQLLVRSLWN 505

[Close]

BLAST of Cla020749 vs. NCBI nr
Match: gi|659133184|ref|XP_008466599.1| (PREDICTED: cytochrome P450 734A6-like [Cucumis melo])

HSP 1 Score: 203.8 bits (517), Expect = 5.3e-49
Identity = 101/165 (61.21%), Postives = 118/165 (71.52%), Query Frame = 1


Query: 2   IKGPPYRPITGNTNNIHRLDAEAQEKA-----------MPYIHQWSEEYGKTFVYWFGST 61
           I GP YRPI GN  ++ R+  EAQ K            +PYIHQWS EYGK FVYWFGS 
Sbjct: 38  ITGPSYRPIIGNATDMRRMYMEAQAKTIPLTHDIVCRVLPYIHQWSMEYGKMFVYWFGSK 97

Query: 62  PRLTISDLDVIKEVLMNTNGSFTKTKASRFVKFLIGKGLAGLDGEKWALHRRIANQAFTV 121
           PRL ISD  +IKEVL NT G F K   +   K L G+GL GL+ E+W +HRRIANQAFT+
Sbjct: 98  PRLAISDPVMIKEVLANTGGPFRKVGFTAVSKLLFGEGLVGLEDEQWVVHRRIANQAFTI 157

Query: 122 ERVKEWLPEIRLSIYSVLEKWEEMKGGKEEFELDVRKQLRLLSAD 156
           +RVK WLPEI LS+ +VL+KWEEMKGG EEFE+DV KQLRLL+AD
Sbjct: 158 DRVKGWLPEITLSVRNVLDKWEEMKGGMEEFEVDVHKQLRLLTAD 202

HSP 2 Score: 344.0 bits (881), Expect = 3.3e-91
Identity = 171/250 (68.40%), Postives = 204/250 (81.60%), Query Frame = 1


Query: 119 EIRLSIYSVLEKWEEMKGGKEEFELDVRKQLRLLSADNENGEEEMLGVEEIMDEYKTFYF 178
           E R SI +++E  +  K  +    L     L L S  N+NGE E L V+E+++E KTFYF
Sbjct: 202 ETRASIKALVESEKNRKERENSTNL---LSLLLSSYKNQNGEIENLEVDEVVNECKTFYF 261

Query: 179 AGMETTANLLTWALLPLAQHQEWQHRAREEVIKVCGHKTPPSSNHLTQLKLMSMIINETL 238
           AGMETTANLLTWALL LA+HQEWQ RAREEVI VCG KTPP++++LT+LKL+ MI+NETL
Sbjct: 262 AGMETTANLLTWALLLLAEHQEWQDRAREEVINVCGQKTPPTADNLTELKLVGMIVNETL 321

Query: 239 LLYPPALLLGPRITKGMTLGSIDVPKGTQLQIVVAAIHHDKEIWGEDASEFNPPRFSAPR 298
            LYPPA+++  R  K +TLG+IDVP+GTQLQ+ V AIHHDKE+WGEDA  FNP RFS PR
Sbjct: 322 RLYPPAIMMMRRTMKRLTLGNIDVPEGTQLQLSVVAIHHDKELWGEDAHNFNPMRFSEPR 381

Query: 299 KHLASFMPFGLGPRICVGQHLALIEAKVALAMIIQRFSFTISPTYMHAPVMLVTLQPQFG 358
           KHLASF+PFGLGPRICVGQHLALIEAKVALAMIIQRF+FTISPTY HAP+M V+L PQFG
Sbjct: 382 KHLASFLPFGLGPRICVGQHLALIEAKVALAMIIQRFAFTISPTYTHAPMMFVSLNPQFG 441

Query: 359 VQLLIKSLWN 369
           VQLL++SLW+
Sbjct: 442 VQLLVRSLWS 448

[Close]

BLAST of Cla020749 vs. NCBI nr
Match: gi|778688998|ref|XP_004147812.2| (PREDICTED: cytochrome P450 734A1-like [Cucumis sativus])

HSP 1 Score: 181.4 bits (459), Expect = 2.8e-42
Identity = 85/129 (65.89%), Postives = 101/129 (78.29%), Query Frame = 1


Query: 27  KAMPYIHQWSEEYGKTFVYWFGSTPRLTISDLDVIKEVLMNTNGSFTKTKASRFVKFLIG 86
           + +PYIHQWS EYGK FVYWFG  PRLTISD  +IKE+L NT G F K   +   K L G
Sbjct: 17  RVLPYIHQWSMEYGKMFVYWFGPKPRLTISDPVMIKEILTNTGGPFRKVGFTPVSKLLFG 76

Query: 87  KGLAGLDGEKWALHRRIANQAFTVERVKEWLPEIRLSIYSVLEKWEEMKGGKEEFELDVR 146
           +GL GL+ E+W +HRRIANQAFT++RVK WLPEI LS+ +VL+KWEEMK G EEFE+DV 
Sbjct: 77  EGLVGLEDEQWVVHRRIANQAFTIDRVKGWLPEITLSVRNVLDKWEEMKEGMEEFEVDVH 136

Query: 147 KQLRLLSAD 156
           KQLRLL+AD
Sbjct: 137 KQLRLLTAD 145

HSP 2 Score: 337.8 bits (865), Expect = 2.4e-89
Identity = 167/248 (67.34%), Postives = 201/248 (81.05%), Query Frame = 1


Query: 119 EIRLSIYSVLEKWEEMKGGKEEFELDVRKQLRLLSADNENGEEEMLGVEEIMDEYKTFYF 178
           E R SI +++E  +  K  +    L     L L S  N+NGE E LGV+E+++E KTFYF
Sbjct: 260 ETRASIKALVESEKNRKERENSTNL---LSLLLSSYKNQNGEIETLGVDEVVNECKTFYF 319

Query: 179 AGMETTANLLTWALLPLAQHQEWQHRAREEVIKVCGHKTPPSSNHLTQLKLMSMIINETL 238
           AGMETTANLLTWALL LA+HQEWQ RAREE++ VCG KTPP++++LT+LKL+ MI+NETL
Sbjct: 320 AGMETTANLLTWALLLLAEHQEWQDRAREEIVNVCGQKTPPTADNLTELKLVGMIVNETL 379

Query: 239 LLYPPALLLGPRITKGMTLGSIDVPKGTQLQIVVAAIHHDKEIWGEDASEFNPPRFSAPR 298
            LYPPA+++  R  K +TLGSID+P+GTQLQ+ V AIHHD E+WGEDA  FNP RFS  R
Sbjct: 380 RLYPPAIMMMRRTMKRLTLGSIDIPEGTQLQLSVVAIHHDTELWGEDAHNFNPKRFSESR 439

Query: 299 KHLASFMPFGLGPRICVGQHLALIEAKVALAMIIQRFSFTISPTYMHAPVMLVTLQPQFG 358
           KHLASF+PFGLGPRICVGQHLALIEAKVALAMIIQRF+FTISPTY HAP+M V+L PQFG
Sbjct: 440 KHLASFLPFGLGPRICVGQHLALIEAKVALAMIIQRFAFTISPTYTHAPMMFVSLNPQFG 499

Query: 359 VQLLIKSL 367
           VQLL++SL
Sbjct: 500 VQLLVRSL 504

The following BLAST results are available for this feature:

BLAST of Cla020749 vs. Swiss-Prot


Match Name	E-value	Identity	Description
C734A_ARATH	1.8e-57	43.56	Cytochrome P450 734A1 OS=Arabidopsis thaliana GN=CYP734A1 PE=2 SV=1	[more]
C7345_ORYSJ	5.3e-57	50.98	Cytochrome P450 734A5 OS=Oryza sativa subsp. japonica GN=CYP734A5 PE=2 SV=1	[more]
C7A29_PANGI	5.9e-56	43.07	Cytochrome P450 CYP72A219 OS=Panax ginseng PE=2 SV=1	[more]
C7A15_ARATH	1.7e-55	49.09	Cytochrome P450 72A15 OS=Arabidopsis thaliana GN=CYP72A15 PE=2 SV=1	[more]
C7344_ORYSJ	1.1e-54	42.11	Cytochrome P450 734A4 OS=Oryza sativa subsp. japonica GN=CYP734A4 PE=2 SV=1	[more]

BLAST of Cla020749 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A067H0N4_CITSI	1.7e-110	49.76	Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g012459mg PE=3 SV=1	[more]
A0A0A0LD75_CUCSA	2.3e-83	69.44	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G855340 PE=3 SV=1	[more]
A0A0A0LD75_CUCSA	6.3e-49	46.18	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G855340 PE=3 SV=1	[more]
A0A164ZR13_DAUCA	1.8e-27	35.91	Uncharacterized protein OS=Daucus carota subsp. sativus GN=DCAR_019516 PE=4 SV=1	[more]
A0A151QX44_CAJCA	6.6e-38	48.80	Secologanin synthase OS=Cajanus cajan GN=KK1_044052 PE=3 SV=1	[more]

BLAST of Cla020749 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|641865795\|gb\|KDO84480.1\|	2.4e-110	49.76	hypothetical protein CISIN_1g012459mg [Citrus sinensis]	[more]
gi\|778688995\|ref\|XP_004147813.2\|	1.2e-93	70.40	PREDICTED: cytochrome P450 734A6-like [Cucumis sativus]	[more]
gi\|778688995\|ref\|XP_004147813.2\|	1.9e-46	59.39	PREDICTED: cytochrome P450 734A6-like [Cucumis sativus]	[more]
gi\|659133184\|ref\|XP_008466599.1\|	5.3e-49	61.21	PREDICTED: cytochrome P450 734A6-like [Cucumis melo]	[more]
gi\|778688998\|ref\|XP_004147812.2\|	2.8e-42	65.89	PREDICTED: cytochrome P450 734A1-like [Cucumis sativus]	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **INTERPRO**
Term	Definition
IPR001128	Cyt_P450
IPR002403	Cyt_P450_E_grp-IV
IPR017972	Cyt_P450_CS

Vocabulary: **Molecular Function**
Term	Definition
GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
GO:0005506	iron ion binding
GO:0020037	heme binding
GO:0004497	monooxygenase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0055114	oxidation-reduction process

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055114	oxidation-reduction process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cla020749	Cla020749	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cla020749	Cla020749.1-protein	polypeptide

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cla020749.1.cds1	Cla020749.1.cds1	CDS
Cla020749.1.cds2	Cla020749.1.cds2	CDS
Cla020749.1.cds3	Cla020749.1.cds3	CDS
Cla020749.1.cds4	Cla020749.1.cds4	CDS

InterPro

Analysis Name: InterPro Annotations of watermelon (97103)
Date Performed: 2016-09-28


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 232..243 score: 8.9E-14coord: 305..314 score: 8.9E-14coord: 314..325 score: 8.9E-14coord: 179..196 score: 8.9
IPR001128	Cytochrome P450	GENE3D	G3DSA:1.10.630.10		coord: 153..365 score: 2.2E-67coord: 2..152 score: 4.4
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 4..150 score: 8.0E-14coord: 151..342 score: 2.2
IPR001128	Cytochrome P450	unknown	SSF48264	Cytochrome P450	coord: 2..364 score: 1.09
IPR002403	Cytochrome P450, E-class, group IV	PRINTS	PR00465	EP450IV	coord: 314..332 score: 1.9E-8coord: 227..243 score: 1.9E-8coord: 170..196 score: 1.9E-8coord: 298..314 score: 1.
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 307..316 scor
None	No IPR available	PANTHER	PTHR24282	CYTOCHROME P450 FAMILY MEMBER	coord: 2..368 score: 3.7E
None	No IPR available	PANTHER	PTHR24282:SF45	CYTOCHROME P450, FAMILY 721, SUBFAMILY A, POLYPEPTIDE 1	coord: 2..368 score: 3.7E