Cla016474.1 (mRNA) Watermelon (97103) v1

BLAST of Cla016474 vs. Swiss-Prot
Match: PROD1_ARATH (Proline dehydrogenase 1, mitochondrial OS=Arabidopsis thaliana GN=POX1 PE=2 SV=2)

HSP 1 Score: 525.8 bits (1353), Expect = 5.1e-148
Identity = 267/464 (57.54%), Postives = 336/464 (72.41%), Query Frame = 1

Query: 26  NTAASSPAAAFTAAATSPPPPINLTDSPATPTVDFTDTRTLFGSLSTSELLRAAANLHVA 85
           +TA      +F   A  PP         +   +D +D   LF S+ TS+LLR+ A LH A
Sbjct: 31  STAVVPEILSFGQQAPEPPLHHPKPTEQSHDGLDLSDQARLFSSIPTSDLLRSTAVLHAA 90

Query: 86  AIGPVVDVGMWVMNSKLMDVEVIRDVVLGTVKHTFYRHFCAGEDDAAVAKTVRRLHDA-G 145
           AIGP+VD+G WVM+SKLMD  V R +VLG VK TFY HFCAGED  A A+ VR +++A G
Sbjct: 91  AIGPMVDLGTWVMSSKLMDASVTRGMVLGLVKSTFYDHFCAGEDADAAAERVRSVYEATG 150

Query: 146 LRSMLDYALEYADDEASCDRNLDGFLRTIEATRSLPPGSASFVVTKVSAICPLRLLERVS 205
           L+ ML Y +E+ADD  SCD N+  F+RTIEA +SLP    S VV K++AICP+ LL+RVS
Sbjct: 151 LKGMLVYGVEHADDAVSCDDNMQQFIRTIEAAKSLPTSHFSSVVVKITAICPISLLKRVS 210

Query: 206 DLLRWQQKNPSFNLPWKLKTLPIFSESSPLYHTLEQPKHLTHEEEKSLQMSHERLMKICQ 265
           DLLRW+ K+P+F L WKLK+ P+FSESSPLYHT  +P+ LT EEE+ L+ +H R+ +IC+
Sbjct: 211 DLLRWEYKSPNFKLSWKLKSFPVFSESSPLYHTNSEPEPLTAEEERELEAAHGRIQEICR 270

Query: 266 SCVEANVPLAIDAEHTKVQPAIDYFTYSAAIIHNKDHN-PIVYGTIQAYLKDAKDRLLLV 325
            C E+NVPL IDAE T +QPAIDY  YS+AI+ N D + PIVY TIQAYL+DA +RL L 
Sbjct: 271 KCQESNVPLLIDAEDTILQPAIDYMAYSSAIMFNADKDRPIVYNTIQAYLRDAGERLHLA 330

Query: 326 TKEAAKMKVPLGIKLVRGAYMSSESKLASSLGFESPIHNSIQDTHFCYNTCASFLLDNIA 385
            + A K  VP+G KLVRGAYMSSE+ LA SLG +SP+H++IQDTH CYN C +FL++  +
Sbjct: 331 VQNAEKENVPMGFKLVRGAYMSSEASLADSLGCKSPVHDTIQDTHSCYNDCMTFLMEKAS 390

Query: 386 KGSS-GAILATHNVESGKLAASKAYEIGIGKMKQKLEFAQLYGMSEALSFGLRNAGFQVS 445
            GS  G +LATHN +SG+LA+ KA ++GI K   K+EFAQLYGMS+ALSFGL+ AGF VS
Sbjct: 391 NGSGFGVVLATHNADSGRLASRKASDLGIDKQNGKIEFAQLYGMSDALSFGLKRAGFNVS 450

Query: 446 KYMPFGPVDMVMPYLLRRAEENRGLLSASNLDRQLMRKELGRRM 487
           KYMPFGPV   +PYLLRRA ENRG+++    DRQLMR EL RR+
Sbjct: 451 KYMPFGPVATAIPYLLRRAYENRGMMATGAHDRQLMRMELKRRL 494

BLAST of Cla016474 vs. Swiss-Prot
Match: PROD2_ARATH (Proline dehydrogenase 2, mitochondrial OS=Arabidopsis thaliana GN=POX2 PE=2 SV=1)

HSP 1 Score: 521.9 bits (1343), Expect = 7.4e-147
Identity = 264/458 (57.64%), Postives = 332/458 (72.49%), Query Frame = 1

Query: 37  TAAATSPP----PPINLTDSPATPT-VDFTDTRTLFGSLSTSELLRAAANLHVAAIGPVV 96
           T +   PP    P I   D P     +D +D   LF S+  S LLR+ A LH  +IGP+V
Sbjct: 16  TVSPVGPPTTIIPEILSFDQPKPEVDLDLSDQARLFASVPISTLLRSTAILHATSIGPMV 75

Query: 97  DVGMWVMNSKLMDVEVIRDVVLGTVKHTFYRHFCAGEDDAAVAKTVRRLHDA-GLRSMLD 156
           D+G W+M+SKLMD  V RD+VL  VK TFY HFCAGED AA A+ V  ++++ GL+ ML 
Sbjct: 76  DLGSWLMSSKLMDTTVTRDLVLRIVKGTFYDHFCAGEDAAAAARRVSSVYESTGLKGMLV 135

Query: 157 YALEYADDEASCDRNLDGFLRTIEATRSLPPGSASFVVTKVSAICPLRLLERVSDLLRWQ 216
           Y +E+A+D  +CD N+  F+ T+EA ++LP    S VV K++AICP+ +L+RVSDLLRWQ
Sbjct: 136 YGVEHAEDGGACDENIQKFIETVEAAKTLPSSHLSSVVVKITAICPMNVLKRVSDLLRWQ 195

Query: 217 QKNPSFNLPWKLKTLPIFSESSPLYHTLEQPKHLTHEEEKSLQMSHERLMKICQSCVEAN 276
            KNP+F LPWKL + P+FS  SPLYHT  +P+ LT EEE+ L+ +HERL  +C  C E+N
Sbjct: 196 YKNPNFKLPWKLNSFPVFSGLSPLYHTTSEPEPLTVEEERELEKAHERLKSVCLRCQESN 255

Query: 277 VPLAIDAEHTKVQPAIDYFTYSAAIIHNKDHN-PIVYGTIQAYLKDAKDRLLLVTKEAAK 336
           VPL IDAE T +QPAIDY  Y +AI+ N D + PIVY TIQAYLKDA +RL L  +E+ K
Sbjct: 256 VPLLIDAEDTILQPAIDYMAYWSAIMFNSDKDRPIVYNTIQAYLKDAGERLHLALRESEK 315

Query: 337 MKVPLGIKLVRGAYMSSESKLASSLGFESPIHNSIQDTHFCYNTCASFLLDNIAKGSS-G 396
           M VP+G KLVRGAYMSSE+KLA SLG++SP+H++IQ+TH CYN C SFL++  + GS   
Sbjct: 316 MNVPIGFKLVRGAYMSSEAKLADSLGYKSPVHDTIQNTHDCYNDCMSFLMEKASNGSGIA 375

Query: 397 AILATHNVESGKLAASKAYEIGIGKMKQKLEFAQLYGMSEALSFGLRNAGFQVSKYMPFG 456
            ILATHN +SGKL A KA E+GI K   K+EFAQLYGMS+ALSFGL+ AGF VSKYMP+G
Sbjct: 376 VILATHNTDSGKLGARKASELGINKENGKIEFAQLYGMSDALSFGLKRAGFNVSKYMPYG 435

Query: 457 PVDMVMPYLLRRAEENRGLLSASNLDRQLMRKELGRRM 487
           PVD  +PYL+RRA ENRG++S   LDRQLMRKEL RR+
Sbjct: 436 PVDTAIPYLIRRAYENRGMMSTGALDRQLMRKELKRRV 473

BLAST of Cla016474 vs. Swiss-Prot
Match: PROD_DICDI (Proline dehydrogenase 1, mitochondrial OS=Dictyostelium discoideum GN=prodh PE=3 SV=1)

HSP 1 Score: 205.7 bits (522), Expect = 1.2e-51
Identity = 147/441 (33.33%), Postives = 225/441 (51.02%), Query Frame = 1

Query: 62  DTRTLFGSLSTSELLRAAANLHVAAIGPVVDVGMWVMNSKLMDVEVIRDVVLGTVKHTFY 121
           DT  L+ S ST EL      L V +I  + D     +N  L +   +   +   +K++F+
Sbjct: 145 DTSKLYVSKSTGELFFTFMILKVCSINFISDNSQKFLN--LFEKLGLSKPLNFFIKYSFF 204

Query: 122 RHFCAGEDDAAVAKTVRRLHDAGLRSMLDYALE-YADDEASCDRNLDGFLRTIEATRSLP 181
           + FCAGE          +L+  G+ ++LDYA+E  A      D   +    TI      P
Sbjct: 205 KQFCAGETIRETEIFTEKLNKLGIGTILDYAIEELAGSSEGFDSVAENICETIRVAAKNP 264

Query: 182 PGSASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLKTLPIFSESSPLYHTLEQ 241
             S S V  K + +    +LE+++ L+         N+   +  LPI S +SPL   L Q
Sbjct: 265 TNSFSCV--KFTGLVTPSVLEKMNTLVS--------NVTTNVSELPIESFNSPLDFYLNQ 324

Query: 242 PKHL---------THEEEKSLQMSHERLMKICQSCVEANVPLAIDAEHTKVQPAIDYFTY 301
              L         T ++ K ++    R+ KI Q C +  VP+ +DAE +  Q AI + T 
Sbjct: 325 SSSLMKQGSEPLLTSKDIKEIKEFFNRMDKIFQLCHQRGVPILVDAEQSYYQVAIHHLTM 384

Query: 302 SAAIIHNKDHNPIVYGTIQAYLKDAKDRLL--LVTKEAAKMKVPLGIKLVRGAYMSSESK 361
           S +I +NK+  PI+Y T Q YL +  + L        + K    LG K+VRGAYM +ES+
Sbjct: 385 SYSIKYNKE-KPIIYNTYQMYLVNGMNVLKQHFELSSSQKFNFKLGAKIVRGAYMVTESE 444

Query: 362 LASSLGFESPIHNSIQDTHFCYNTCASFLLDNIAK--GSSGAILATHNVESGKLAASKAY 421
            +  L  E+P+  +IQDTH  YNT   FLL+ I     S G ++A+HN +S  L      
Sbjct: 445 RSQRLSTENPVLPTIQDTHKSYNTALDFLLNQIKSDPNSIGLMIASHNEDSINLGTKLIK 504

Query: 422 EIGIGKMKQKLEFAQLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGL 481
           +  I      ++F QL+GM++ LSF L +   ++ KY+PFGPV+ V+PYL+RR  EN+G 
Sbjct: 505 QYKIDPTNPNIQFGQLFGMADFLSFNLVDQHQRIFKYVPFGPVEEVLPYLIRRMHENKGF 564

Query: 482 LSASNLDRQL--MRKELGRRM 487
           +  SN D++L  ++KE+ RR+
Sbjct: 565 I-GSNSDKELFYLKKEIKRRL 571

BLAST of Cla016474 vs. Swiss-Prot
Match: PROD2_HUMAN (Probable proline dehydrogenase 2 OS=Homo sapiens GN=PRODH2 PE=2 SV=1)

HSP 1 Score: 167.9 bits (424), Expect = 2.7e-40
Identity = 132/431 (30.63%), Postives = 211/431 (48.96%), Query Frame = 1

Query: 72  TSELLRAAANLHVAAIGPVVDVGMWVM--NSKLMDVEVIRDVVLGTVKHTFYRHFCAGED 131
           T EL RA   L + A  P+V  G+ +   + +L+   +    +  +V    Y  F AGE 
Sbjct: 110 TGELTRALLVLRLCAWPPLVTHGLLLQAWSRRLLGSRLSGAFLRASV----YGQFVAGET 169

Query: 132 DAAVAKTVRRLHDAGLRSMLDYALEYADD------EASCDRNLDGFLRTIEATRSL--PP 191
              V   V++L    LR +L    E   D      EA  + NL   LR ++ +R L  PP
Sbjct: 170 AEEVKGCVQQLRTLSLRPLLAVPTEEEPDSAAKSGEAWYEGNLGAMLRCVDLSRGLLEPP 229

Query: 192 G--SASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLKTLPIFSESSPLYHTLE 251
               AS +  KV+A+   RL + ++  +R     P  +L    + L    +S        
Sbjct: 230 SLAEASLMQLKVTALTSTRLCKELASWVR----RPGASLELSPERLAEAMDSGQNL---- 289

Query: 252 QPKHLTHEEEKSLQMSHERLMKICQSCVEANVPLAIDAEHTKVQPAIDYFTYSAAIIHNK 311
           Q   L  E+ + L+ S  RL ++ Q     +V L +DAE+T + PA+     + A+  N 
Sbjct: 290 QVSCLNAEQNQHLRASLSRLHRVAQYARAQHVRLLVDAEYTSLNPALSLLVAALAVRWNS 349

Query: 312 --DHNPIVYGTIQAYLKDAKDRLLLVTKEAAKMKVPLGIKLVRGAYMSSESKLASSLGFE 371
             +  P V+ T QA LKD  +RL    + A +  +  G+KLVRGAY+  E  +A   G E
Sbjct: 350 PGEGGPWVWNTYQACLKDTFERLGRDAEAAHRAGLAFGVKLVRGAYLDKERAVAQLHGME 409

Query: 372 SPIHNSIQDTHFCYNTCASFLLDNIAKGSSGA--ILATHNVESGKLAASKAYEIGIGKMK 431
            P     + T   Y+ C   +L ++A+       ++A+HN ES + A  + +E+GI  + 
Sbjct: 410 DPTQPDYEATSQSYSRCLELMLTHVARHGPMCHLMVASHNEESVRQATKRMWELGI-PLD 469

Query: 432 QKLEFAQLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSASNLDR 487
             + F QL GM + +S  L  AG+ V K +P+G ++ V+PYL+RRA+ENR +L  +  ++
Sbjct: 470 GTVCFGQLLGMCDHVSLALGQAGYVVYKSIPYGSLEEVIPYLIRRAQENRSVLQGARREQ 527

BLAST of Cla016474 vs. Swiss-Prot
Match: PROD2_BOVIN (Probable proline dehydrogenase 2 OS=Bos taurus GN=PRODH2 PE=2 SV=1)

HSP 1 Score: 164.5 bits (415), Expect = 3.0e-39
Identity = 130/433 (30.02%), Postives = 210/433 (48.50%), Query Frame = 1

Query: 71  STSELLRAAANLHVAAIGPVVDVGMWVM--NSKLMDVEVIRDVVLGTVKHTFYRHFCAGE 130
           S  EL RA   L + A  P+V  G+ +   + +L+   +   ++  ++    Y  F AGE
Sbjct: 33  SIGELTRALLVLRLCAWPPLVTHGLALQAWSQRLLGSRLSGALLRASI----YGQFVAGE 92

Query: 131 DDAAVAKTVRRLHDAGLRSMLDYALEYADD------EASCDRNLDGFLRTIEATRSL--- 190
               V   V +L + GLR +L    E   D      EA  + NL   LR ++ +R L   
Sbjct: 93  TAEEVRSCVLQLQNLGLRPLLAVPTEEEPDSAVKTGEAWYEGNLSAMLRCVDLSRGLLET 152

Query: 191 --PPGSASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLKTLPIFSESSPLYHT 250
             P G+A  +  K++A+   RL ++++  +R     P  +L    + L    +S      
Sbjct: 153 PDPTGNA-LMQLKMTALMSTRLCKQLTSWVR----RPGDSLELSPERLAEAMDSGQDL-- 212

Query: 251 LEQPKHLTHEEEKSLQMSHERLMKICQSCVEANVPLAIDAEHTKVQPAIDYFTYSAAIIH 310
             Q   L  E+ + LQ S  RL ++ Q      V L +DAE+T + PA+     + A   
Sbjct: 213 --QVSCLNTEQTRHLQASLSRLHRVVQHARAQRVRLLVDAEYTSLNPALSLLVAALATRW 272

Query: 311 NK--DHNPIVYGTIQAYLKDAKDRLLLVTKEAAKMKVPLGIKLVRGAYMSSESKLASSLG 370
           N   +  P V+ T QAYLKD  +RL    + A +  +  G+KLVRGAY+  E + A   G
Sbjct: 273 NSSGEGGPWVWNTYQAYLKDTYERLRWDAEAADRAGLAFGVKLVRGAYLDKERETARLQG 332

Query: 371 FESPIHNSIQDTHFCYNTCASFLLDNIAKGSSGA--ILATHNVESGKLAASKAYEIGIGK 430
            E P     + T   Y+ C   +L  ++        ++A+HN +S + A  + +E+GI  
Sbjct: 333 TEDPTQPDYEATSQSYSRCLELMLTQVSHRGPMCHLMVASHNEDSVRQATKRMWELGIPP 392

Query: 431 MKQKLEFAQLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSASNL 487
               + F QL GM + +S  L  AG+ V K +P+G ++ V+PYL+RRA+ENR +L  +  
Sbjct: 393 -DGPVCFGQLLGMCDHVSLALGQAGYAVYKSIPYGSLEEVIPYLIRRAQENRSVLRGARR 451

BLAST of Cla016474 vs. TrEMBL
Match: A0A0H4CNX3_BETPL (Proline dehydrogenase OS=Betula platyphylla GN=ProDH PE=2 SV=1)

HSP 1 Score: 627.5 bits (1617), Expect = 1.4e-176
Identity = 306/466 (65.67%), Postives = 370/466 (79.40%), Query Frame = 1

Query: 21  FNRSLNTAASSPAAAFTAAATSPPPPINLTDSPATPTVDFTDTRTLFGSLSTSELLRAAA 80
           F R L +++SS ++        P P       P T +++  D   LF S+ TS+LLRA+A
Sbjct: 18  FTRPLTSSSSSSSSVAVNHTVKPEPTFQ--KPPTTTSLELDDAEKLFSSVPTSKLLRASA 77

Query: 81  NLHVAAIGPVVDVGMWVMNSKLMDVEVIRDVVLGTVKHTFYRHFCAGEDDAAVAKTVRRL 140
            LH AAI P+VD G WVM SKLM V VIR  VLG ++HTFY HFCAGED  A  ++VR L
Sbjct: 78  ILHAAAIEPMVDFGTWVMQSKLMHVNVIRGAVLGALRHTFYEHFCAGEDADAAGESVRSL 137

Query: 141 HDAGLRSMLDYALEYADDEASCDRNLDGFLRTIEATRSLPPGSASFVVTKVSAICPLRLL 200
           H  GLR+MLDYA+EYA D  SCDRNL+ FL T+E+  SLPP S SF++ K++AICP+ L+
Sbjct: 138 HRCGLRAMLDYAVEYAGDNESCDRNLEAFLHTVESATSLPPSSVSFLIVKITAICPMELV 197

Query: 201 ERVSDLLRWQQKNPSFNLPWKLKTLPIFSESSPLYHTLEQPKHLTHEEEKSLQMSHERLM 260
           ERVSDLLRWQ K+PSFNLPWK  TLPI S+SSP Y TL++P+ LT EEE+ LQ+ H+RL+
Sbjct: 198 ERVSDLLRWQYKDPSFNLPWKQDTLPILSDSSPTYQTLKKPEPLTPEEERDLQLGHQRLL 257

Query: 261 KICQSCVEANVPLAIDAEHTKVQPAIDYFTYSAAIIHNKDHNPIVYGTIQAYLKDAKDRL 320
           KICQ CVEANVPLA+DAE+T +QPAIDYFTYS+AI+ NKD +PIV+GTIQAYLKDAK+RL
Sbjct: 258 KICQKCVEANVPLAVDAEYTSIQPAIDYFTYSSAIMCNKDEHPIVHGTIQAYLKDAKERL 317

Query: 321 LLVTKEAAKMKVPLGIKLVRGAYMSSESKLASSLGFESPIHNSIQDTHFCYNTCASFLLD 380
           LL +  A KM  P+G KLVRGAYMSSE KLAS+LGFESPIH SI +TH CYN CASFLL+
Sbjct: 318 LLASMAADKMGFPMGFKLVRGAYMSSERKLASALGFESPIHKSIHETHVCYNDCASFLLE 377

Query: 381 NIAKGSSGAILATHNVESGKLAASKAYEIGIGKMKQKLEFAQLYGMSEALSFGLRNAGFQ 440
            I+ G    +LATHNVESG++A +KA+++GIGK+  K++FAQLYGM++ALSFGLRNAGFQ
Sbjct: 378 KISNGPGALVLATHNVESGRIAVAKAHDLGIGKVNHKMQFAQLYGMADALSFGLRNAGFQ 437

Query: 441 VSKYMPFGPVDMVMPYLLRRAEENRGLLSASNLDRQLMRKELGRRM 487
           VSKYMPFGPV+MVMPYLLRRAEENRGLLSAS LDRQLMRKEL RR+
Sbjct: 438 VSKYMPFGPVEMVMPYLLRRAEENRGLLSASTLDRQLMRKELKRRL 481

BLAST of Cla016474 vs. TrEMBL
Match: M5X4N6_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa004722mg PE=4 SV=1)

HSP 1 Score: 627.1 bits (1616), Expect = 1.8e-176
Identity = 314/500 (62.80%), Postives = 387/500 (77.40%), Query Frame = 1

Query: 1   MARSIVSTQSKISKKVFFYAFNRSLNTAASSPAAAFTAAATSPP-----PPINLTDSPAT 60
           MA  ++   SK+ K +      R LN+AASS     T +A SPP      P+      A 
Sbjct: 1   MANRVIPAASKLLKTL------RPLNSAASST----TISAISPPIGYAEKPLQSPSGHAI 60

Query: 61  PTVD--------FTDTRTLFGSLSTSELLRAAANLHVAAIGPVVDVGMWVMNSKLMDVEV 120
              D          D   LF S+ST  LLRAA NLH  A  P+VD+GMWVM S+LMD  +
Sbjct: 61  TAADTITDNIFNLDDVDKLFASVSTPTLLRAALNLHAVAFEPLVDIGMWVMKSRLMDTPL 120

Query: 121 IRDVVLGTVKHTFYRHFCAGEDDAAVAKTVRRLHDAGLRSMLDYALEYADDEASCDRNLD 180
           IRDV++G ++ TFY HFCAGE+  A  ++V  L++AGLR ML YALEYA D  +CDRNL 
Sbjct: 121 IRDVIMGIIRSTFYEHFCAGENAMAAGRSVLELNEAGLRGMLVYALEYAGDNDACDRNLQ 180

Query: 181 GFLRTIEATRSLPPGSASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLKTLPI 240
           GFL T EAT+SLPP S SF++ K++AICP+R+LERVSDLLRWQ K+PSF LPWKL TLPI
Sbjct: 181 GFLDTAEATKSLPPSSVSFIIMKITAICPMRVLERVSDLLRWQHKDPSFKLPWKLDTLPI 240

Query: 241 FSESSPLYHTLEQPKHLTHEEEKSLQMSHERLMKICQSCVEANVPLAIDAEHTKVQPAID 300
           FS++SPLYHTLE+P+ LT +EE+ L++ H+RL+K+ Q C EANVPL++DAE++ VQPAID
Sbjct: 241 FSDNSPLYHTLEKPEPLTPDEERDLELVHQRLLKLSQKCSEANVPLSVDAEYSSVQPAID 300

Query: 301 YFTYSAAIIHNKDHNPIVYGTIQAYLKDAKDRLLLVTKEAAKMKVPLGIKLVRGAYMSSE 360
           Y TYS+AII+NKD NPIVYGTIQAYLKDAK+RLLL TK A  M +P+G K+VRGAYMSSE
Sbjct: 301 YLTYSSAIIYNKDDNPIVYGTIQAYLKDAKERLLLATKAADDMGIPMGFKVVRGAYMSSE 360

Query: 361 SKLASSLGFESPIHNSIQDTHFCYNTCASFLLDNIAKGSSGAILATHNVESGKLAASKAY 420
           +K+ASSLG++SPIH+SI+DTH CYN CASF+L+ IA GS G +LATHNVESGKLA +KA+
Sbjct: 361 AKVASSLGYKSPIHDSIEDTHACYNDCASFMLEKIANGSGGVVLATHNVESGKLAVAKAH 420

Query: 421 EIGIGKMKQKLEFAQLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGL 480
           EIGIGK++QK+EFAQLYGM+E+LSFGLRNAGFQVSKYMPFGP+++V+PYLLRRAEENRGL
Sbjct: 421 EIGIGKVRQKIEFAQLYGMAESLSFGLRNAGFQVSKYMPFGPIELVLPYLLRRAEENRGL 480

Query: 481 LSASNLDRQLMRKELGRRMK 488
           LSAS  DRQ  R+ L RR+K
Sbjct: 481 LSASTQDRQRTREVLKRRLK 490

BLAST of Cla016474 vs. TrEMBL
Match: A0A061EI66_THECC (Methylenetetrahydrofolate reductase family protein isoform 1 OS=Theobroma cacao GN=TCM_019628 PE=4 SV=1)

HSP 1 Score: 621.7 bits (1602), Expect = 7.6e-175
Identity = 306/477 (64.15%), Postives = 376/477 (78.83%), Query Frame = 1

Query: 21  FNRSLNTAASSPAAAFT----------AAATSPPPPINLTDSPATPTVDFTDTRTLFGSL 80
           F R LN+A+S+   A +          AAA    PPIN   +    T D  D + LF S+
Sbjct: 17  FTRPLNSASSASITAVSPLNFDEKPEPAAAAIEKPPINQPSA----TFDLNDHQKLFDSV 76

Query: 81  STSELLRAAANLHVAAIGPVVDVGMWVMNSKLMDVEVIRDVVLGTVKHTFYRHFCAGEDD 140
           ST +LLR++ANL +A+I P VD GMWVMNS+LM+  +IRDV+L TVKHTF+ HFCAGE+ 
Sbjct: 77  STLKLLRSSANLALASIEPFVDFGMWVMNSRLMETSLIRDVILKTVKHTFFEHFCAGENT 136

Query: 141 AAVAKTVRRLHDAGLRSMLDYALEYADDEASCDRNLDGFLRTIEATRSLPPGSASFVVTK 200
               + VR++H+AGLR ML YA+E+  D A C+RNL+ FLR++E  +SLPP S SFV+ K
Sbjct: 137 KEAGECVRKIHEAGLRGMLGYAVEHTSDNAGCERNLEAFLRSVEFAKSLPPSSVSFVIAK 196

Query: 201 VSAICPLRLLERVSDLLRWQQKNPSFNLPWKLKTLPIFSESSPLYHTLEQPKHLTHEEEK 260
           ++AICP+ L+ RVSDLLRWQ K+PSFNLPWKL T PIFS+SSPLYHTL++P+ LT +EE 
Sbjct: 197 ITAICPINLVRRVSDLLRWQYKDPSFNLPWKLNTFPIFSDSSPLYHTLQRPEPLTPQEEL 256

Query: 261 SLQMSHERLMKICQSCVEANVPLAIDAEHTKVQPAIDYFTYSAAIIHNKDHNPIVYGTIQ 320
             Q++H+RL+K+CQ CVE NV L IDAE T +QPAIDY TYS+A+++N+D NPIVYGTIQ
Sbjct: 257 DFQLAHQRLLKLCQKCVEDNVALTIDAEDTSLQPAIDYLTYSSALMYNRDDNPIVYGTIQ 316

Query: 321 AYLKDAKDRLLLVTKEAAKMKVPLGIKLVRGAYMSSESKLASSLGFESPIHNSIQDTHFC 380
           AYLKDAK+RL L  K A K+ +P+G KLVRGAYM+SE KLASSLG++SPIHNSIQ+TH C
Sbjct: 317 AYLKDAKERLFLAEKTAEKLGIPMGFKLVRGAYMTSERKLASSLGYDSPIHNSIQETHAC 376

Query: 381 YNTCASFLLDNIAKGSSGAILATHNVESGKLAASKAYEIGIGKMKQKLEFAQLYGMSEAL 440
           YN CASF+L+ IA G    +LATHNVESG+LAASKA ++GI K  QKLEFAQLYGMSEAL
Sbjct: 377 YNDCASFMLERIADGHGAVVLATHNVESGQLAASKARDLGIQKGNQKLEFAQLYGMSEAL 436

Query: 441 SFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSASNLDRQLMRKELGRRMK 488
           SFGLRNAGFQVSKY+P+GPVDMVMPYLLRRAEENRGLLS S+LDR+LM KEL RR+K
Sbjct: 437 SFGLRNAGFQVSKYLPYGPVDMVMPYLLRRAEENRGLLSTSSLDRELMGKELKRRLK 489

BLAST of Cla016474 vs. TrEMBL
Match: V9M5A2_MANES (Proline oxidase OS=Manihot esculenta GN=ProDH PE=2 SV=1)

HSP 1 Score: 619.8 bits (1597), Expect = 2.9e-174
Identity = 316/491 (64.36%), Postives = 389/491 (79.23%), Query Frame = 1

Query: 7   STQSKISKKVFFY--AFNRSLNTAASSPAAAFTAAATSPPPPINLTDSPATPTV------ 66
           +T   IS K+  +  +F R L T++SS +++  +++ S  P +NL   P  PT+      
Sbjct: 5   TTTRVISPKLLKHISSFIRPLVTSSSSTSSS--SSSISAIPSLNLATKPE-PTITTPPSI 64

Query: 67  -DFTDTRTLFGSLSTSELLRAAANLHVAAIGPVVDVGMWVMNSKLMDVE-VIRDVVLGTV 126
            D +D + LF +L + +LL A+  LH+AA+ PVVD+GMWVMNSKLM V+ ++RD++L  V
Sbjct: 65  LDLSDHQKLFATLPSRKLLHASLILHMAAMNPVVDLGMWVMNSKLMGVDNIVRDLILSAV 124

Query: 127 KHTFYRHFCAGEDDAAVAKTVRRLHDAGLRSMLDYALEYADDEASCDRNLDGFLRTIEAT 186
           +H+FY HFCAGED   VA  V++L+++GLR MLD+++E+  D  +CDRN  GFL TIE  
Sbjct: 125 RHSFYEHFCAGEDTVEVANCVKKLNESGLRGMLDFSVEFTVDNDACDRNFQGFLDTIETA 184

Query: 187 RSLPPGSASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLKTLPIFSESSPLYH 246
           +SL P S S VV K++AICPL LLERV+DLLRWQ+K+PSFNLPWK  T PIFSES+PLYH
Sbjct: 185 KSLSPSSVSAVVVKITAICPLNLLERVADLLRWQEKDPSFNLPWKQNTFPIFSESTPLYH 244

Query: 247 TLEQPKHLTHEEEKSLQMSHERLMKICQSCVEANVPLAIDAEHTKVQPAIDYFTYSAAII 306
           TL++P+ LT EEE+ L +  ++L+K+CQ+C EANVPL IDAEHTKVQPAIDYFTYS+AI 
Sbjct: 245 TLKKPEPLTPEEEQHLLLGQQKLLKLCQACAEANVPLVIDAEHTKVQPAIDYFTYSSAIK 304

Query: 307 HNKDHNPIVYGTIQAYLKDAKDRLLLVTKEAAKMKVPLGIKLVRGAYMSSESKLASSLGF 366
           +NKD NPIVYGTIQAYLKDAK+RLLL TK A KM +P+G KLVRGAYMSSE KLASSLG+
Sbjct: 305 YNKDDNPIVYGTIQAYLKDAKERLLLATKAADKMGLPMGFKLVRGAYMSSERKLASSLGY 364

Query: 367 ESPIHNSIQDTHFCYNTCASFLLDNIAKGSSGAILATHNVESGKLAASKAYEIGIGKMKQ 426
           +SPIHNSIQ TH CYN CASFLL+ IA  S G ILATHNVESG LAA KA E+GIG   Q
Sbjct: 365 QSPIHNSIQQTHDCYNDCASFLLEAIADRSHGLILATHNVESGTLAAKKAEELGIGNGNQ 424

Query: 427 KLEFAQLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSASNLDRQ 486
           +LEFAQLYGM+EALSFGLRNAGFQVSKY+PFGPV+MV+PYLLRRAEENRGLLSAS LDR+
Sbjct: 425 RLEFAQLYGMAEALSFGLRNAGFQVSKYLPFGPVEMVIPYLLRRAEENRGLLSASGLDRE 484

Query: 487 LMRKELGRRMK 488
           L RKEL RR+K
Sbjct: 485 LTRKELSRRLK 492

BLAST of Cla016474 vs. TrEMBL
Match: W9RE00_9ROSA (Proline dehydrogenase 2 OS=Morus notabilis GN=L484_009682 PE=4 SV=1)

HSP 1 Score: 616.3 bits (1588), Expect = 3.2e-173
Identity = 295/461 (63.99%), Postives = 379/461 (82.21%), Query Frame = 1

Query: 27  TAASSPAAAFTAAATSP----PPPINLTDSPATPTVD----FTDTRTLFGSLSTSELLRA 86
           TA+SS  +A  AAA SP        +L++ P +   D      D   LF ++ T++L+RA
Sbjct: 21  TASSSSPSAAVAAAVSPLIVTSDDADLSERPRSSRSDALLNLDDYEKLFATVPTAKLVRA 80

Query: 87  AANLHVAAIGPVVDVGMWVMNSKLMDVEVIRDVVLGTVKHTFYRHFCAGEDDAAVAKTVR 146
           +ANL  +AI P+VD G+ +M S+LM++EV+RD++LGT++HTFY HFCAGED  A  +TV 
Sbjct: 81  SANLQASAIEPLVDFGIRLMKSRLMEMEVVRDIILGTIRHTFYEHFCAGEDAEAAGRTVL 140

Query: 147 RLHDAGLRSMLDYALEYADDEASCDRNLDGFLRTIEATRSLPPGSASFVVTKVSAICPLR 206
           RL + G+R ML YALEYA +  +CDRNLDGF+RT+++T+SLP  S SF++ K++AICP+R
Sbjct: 141 RLRETGIRGMLVYALEYATENDACDRNLDGFIRTVQSTKSLPSSSTSFIIVKITAICPMR 200

Query: 207 LLERVSDLLRWQQKNPSFNLPWKLKTLPIFSESSPLYHTLEQPKHLTHEEEKSLQMSHER 266
           +LERVSDLLRWQ K+PS +LPWKL + P+FS+SSP+YHTL++P+ L+ EEE+ LQ+ HER
Sbjct: 201 VLERVSDLLRWQHKDPSCHLPWKLNSFPLFSDSSPMYHTLKRPEPLSPEEERDLQLGHER 260

Query: 267 LMKICQSCVEANVPLAIDAEHTKVQPAIDYFTYSAAIIHNKDHNPIVYGTIQAYLKDAKD 326
           L+K+C+ C EANVPL++DAEHT VQPAIDY TY++AI+ NK   PIVYGTIQAYLKDAK+
Sbjct: 261 LLKLCRECAEANVPLSVDAEHTAVQPAIDYLTYASAIMRNKGSYPIVYGTIQAYLKDAKE 320

Query: 327 RLLLVTKEAAKMKVPLGIKLVRGAYMSSESKLASSLGFESPIHNSIQDTHFCYNTCASFL 386
           RLLL ++ A KM +P+G K+VRGAYMSSE+KLA+SLG +SPIHNSIQ+TH CYN CASF+
Sbjct: 321 RLLLASEAADKMGIPMGFKVVRGAYMSSETKLANSLGCDSPIHNSIQETHDCYNDCASFM 380

Query: 387 LDNIAKGSSGAILATHNVESGKLAASKAYEIGIGKMKQKLEFAQLYGMSEALSFGLRNAG 446
           LD IA GS G +LATHNVESGK+AA+KA+++GIGK+ Q+LEFAQLYGM+E+LSFGL+NAG
Sbjct: 381 LDRIANGSGGLVLATHNVESGKMAAAKAHDVGIGKVNQRLEFAQLYGMAESLSFGLKNAG 440

Query: 447 FQVSKYMPFGPVDMVMPYLLRRAEENRGLLSASNLDRQLMR 480
           FQVSKYMPFGP+DMVMPYLLRRAEENRGLLSAS +DRQLMR
Sbjct: 441 FQVSKYMPFGPIDMVMPYLLRRAEENRGLLSASTVDRQLMR 481

BLAST of Cla016474 vs. NCBI nr
Match: gi|778707546|ref|XP_004141603.2| (PREDICTED: proline dehydrogenase 2, mitochondrial-like [Cucumis sativus])

HSP 1 Score: 872.1 bits (2252), Expect = 4.7e-250
Identity = 438/491 (89.21%), Postives = 466/491 (94.91%), Query Frame = 1

Query: 1   MARSIVSTQSKISKKVFFYAFNRSLNTAASSPAAAFTAAA--TSPPPPINLTDSPATPTV 60
           MA++IVSTQSKISK+VFFY FNR LNTAASSPAAAFTAA   TSPP P++L + P+TPTV
Sbjct: 1   MAQAIVSTQSKISKRVFFYVFNRPLNTAASSPAAAFTAATATTSPPSPLHL-NPPSTPTV 60

Query: 61  DFTDTRTLFGSLSTSELLRAAANLHVAAIGPVVDVGMWVMNSKLMDVEVIRDVVLGTVKH 120
           DFTD+R LFGS+ TS+LL A A LH AA+GPVVDVGMWVMNSKLMDVE+ RDVVLGTVKH
Sbjct: 61  DFTDSRALFGSIPTSDLLHATATLHAAAVGPVVDVGMWVMNSKLMDVELFRDVVLGTVKH 120

Query: 121 TFYRHFCAGEDDAAVAKTVRRLHDAGLRSMLDYALEYADDEASCDRNLDGFLRTIEATRS 180
           +FYRHFCAGEDD +VAKTVRRLHD GLRSMLDYALEYADDEASCDRNLDGFL T+EAT+S
Sbjct: 121 SFYRHFCAGEDDTSVAKTVRRLHDVGLRSMLDYALEYADDEASCDRNLDGFLSTVEATKS 180

Query: 181 LPPGSASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLKTLPIFSESSPLYHTL 240
           LP GSASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKL+TLPIFSESSPLYHTL
Sbjct: 181 LPSGSASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLQTLPIFSESSPLYHTL 240

Query: 241 EQPKHLTHEEEKSLQMSHERLMKICQSCVEANVPLAIDAEHTKVQPAIDYFTYSAAIIHN 300
           EQP+ LT EEEKSLQMSHERLMKICQSCV+ANVPLAIDAEHTKVQPAIDYFTYSAAIIHN
Sbjct: 241 EQPEPLTREEEKSLQMSHERLMKICQSCVDANVPLAIDAEHTKVQPAIDYFTYSAAIIHN 300

Query: 301 KDHNPIVYGTIQAYLKDAKDRLLLVTKEAAKMKVPLGIKLVRGAYMSSESKLASSLGFES 360
           KD NPIVYGTIQAYLKDAKDRLLL  KEA+K+KVPLGIKLVRGAYMSSESKLASSLGFES
Sbjct: 301 KDRNPIVYGTIQAYLKDAKDRLLLANKEASKLKVPLGIKLVRGAYMSSESKLASSLGFES 360

Query: 361 PIHNSIQDTHFCYNTCASFLLDNIAKGSSGAILATHNVESGKLAASKAYEIGIGKMKQKL 420
           PIH++IQDTH CYNTCASFLLD+IAKGS+GAILATHNVESGKLAAS+AYEIGIGK+KQKL
Sbjct: 361 PIHDTIQDTHSCYNTCASFLLDDIAKGSTGAILATHNVESGKLAASRAYEIGIGKLKQKL 420

Query: 421 EFAQLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSASNLDRQLM 480
           EFAQLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSASNLDR+LM
Sbjct: 421 EFAQLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSASNLDRELM 480

Query: 481 RKELGRRMKEY 490
           RKELGRRMKEY
Sbjct: 481 RKELGRRMKEY 490

BLAST of Cla016474 vs. NCBI nr
Match: gi|659119046|ref|XP_008459446.1| (PREDICTED: proline dehydrogenase 2, mitochondrial [Cucumis melo])

HSP 1 Score: 859.8 bits (2220), Expect = 2.4e-246
Identity = 430/485 (88.66%), Postives = 459/485 (94.64%), Query Frame = 1

Query: 1   MARSIVSTQSKISKKVFFYAFNRSLNTAASSPAAAFTAAATSPPPPINLTDSPATPTVDF 60
           MAR++VSTQSKISK+VF Y FNR LNTAASSPAAAFTA    PPPP++L + P+TPTVDF
Sbjct: 1   MARALVSTQSKISKRVFLYVFNRPLNTAASSPAAAFTA----PPPPLHL-NPPSTPTVDF 60

Query: 61  TDTRTLFGSLSTSELLRAAANLHVAAIGPVVDVGMWVMNSKLMDVEVIRDVVLGTVKHTF 120
           TD+R LFGS+ TS+LL A A LH +AIGPVVDVGMWVMNSKLMDVE+ RDVVLGTVKHTF
Sbjct: 61  TDSRALFGSIPTSDLLHATATLHASAIGPVVDVGMWVMNSKLMDVELFRDVVLGTVKHTF 120

Query: 121 YRHFCAGEDDAAVAKTVRRLHDAGLRSMLDYALEYADDEASCDRNLDGFLRTIEATRSLP 180
           YRHFCAGEDD +VAKTVRRLHD GLRSMLDYALEYADDEASCDRNLDGFLRT+EAT+SLP
Sbjct: 121 YRHFCAGEDDTSVAKTVRRLHDVGLRSMLDYALEYADDEASCDRNLDGFLRTVEATKSLP 180

Query: 181 PGSASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLKTLPIFSESSPLYHTLEQ 240
            GSASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKL+TLPIFSESSPLYHTLEQ
Sbjct: 181 SGSASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLQTLPIFSESSPLYHTLEQ 240

Query: 241 PKHLTHEEEKSLQMSHERLMKICQSCVEANVPLAIDAEHTKVQPAIDYFTYSAAIIHNKD 300
           P+ LT EEEKSLQ+SHERLMKICQSCV+ANVPLAIDAEHTKVQPAIDYFTYSAAIIHNKD
Sbjct: 241 PEPLTREEEKSLQISHERLMKICQSCVDANVPLAIDAEHTKVQPAIDYFTYSAAIIHNKD 300

Query: 301 HNPIVYGTIQAYLKDAKDRLLLVTKEAAKMKVPLGIKLVRGAYMSSESKLASSLGFESPI 360
            NPIVYGTIQAYLKDAKDRLLL  KEA+K+K+PLGIKLVRGAYMSSESKLASSLGFESPI
Sbjct: 301 RNPIVYGTIQAYLKDAKDRLLLANKEASKLKIPLGIKLVRGAYMSSESKLASSLGFESPI 360

Query: 361 HNSIQDTHFCYNTCASFLLDNIAKGSSGAILATHNVESGKLAASKAYEIGIGKMKQKLEF 420
           HN+IQDTH CYNTCASFLLD+IAKGSSGAILATHNVESGKLAA++AYEIGIGK+KQKLEF
Sbjct: 361 HNTIQDTHSCYNTCASFLLDDIAKGSSGAILATHNVESGKLAATRAYEIGIGKLKQKLEF 420

Query: 421 AQLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSASNLDRQLMRK 480
           AQLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSASNLDRQLMRK
Sbjct: 421 AQLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSASNLDRQLMRK 480

Query: 481 ELGRR 486
           E G++
Sbjct: 481 EFGKK 480

BLAST of Cla016474 vs. NCBI nr
Match: gi|1009127021|ref|XP_015880472.1| (PREDICTED: proline dehydrogenase 2, mitochondrial-like [Ziziphus jujuba])

HSP 1 Score: 633.6 bits (1633), Expect = 2.8e-178
Identity = 308/473 (65.12%), Postives = 385/473 (81.40%), Query Frame = 1

Query: 24  SLNTAASSPAAAFTA------AATSPPPPINLTDSPATPT----VDFTDTRTLFGSLSTS 83
           S ++ +++ A AF+A      A T   P I  T +   P     +DF D   LF S+STS
Sbjct: 32  SSSSTSATAATAFSALNLAGAADTKIEPTITTTTTTTIPKSTSILDFDDLDKLFSSVSTS 91

Query: 84  ELLRAAANLHVAAIGPVVDVGMWVMNSKLMDVEVIRDVVLGTVKHTFYRHFCAGEDDAAV 143
           +LLRA+ANL++A++ P+VD+GMWVM SKLM+ ++++D+VLG +K TF+ HFCAGE+    
Sbjct: 92  KLLRASANLYMASVEPIVDLGMWVMRSKLMEKQLVKDMVLGLIKRTFFEHFCAGENTVEA 151

Query: 144 AKTVRRLHDAGLRSMLDYALEYADDEASCDRNLDGFLRTIEATRSLPPGSASFVVTKVSA 203
             +  RL DAGLR MLDYALE+A D  SCDRN++GFL TIE+T+SLPP   SFVV K+SA
Sbjct: 152 GNSALRLRDAGLRGMLDYALEFACDNESCDRNMEGFLTTIESTKSLPPSIVSFVVVKISA 211

Query: 204 ICPLRLLERVSDLLRWQQKNPSFNLPWKLKTLPIFSESSPLYHTLEQPKHLTHEEEKSLQ 263
           ICPLRLLERVSDLLRWQ KNPS +LPWKL T PIFS+SSP YHTL +P+ LT EEE+ LQ
Sbjct: 212 ICPLRLLERVSDLLRWQHKNPSCSLPWKLNTFPIFSDSSPTYHTLTEPQPLTPEEERDLQ 271

Query: 264 MSHERLMKICQSCVEANVPLAIDAEHTKVQPAIDYFTYSAAIIHNKDHNPIVYGTIQAYL 323
           ++H+RL+ +CQ C+EAN+PL +DAEHT VQPAIDY TYS+AI++NKD+NPIVYGT+QAYL
Sbjct: 272 LAHQRLLTLCQECLEANLPLLVDAEHTAVQPAIDYLTYSSAIMYNKDNNPIVYGTLQAYL 331

Query: 324 KDAKDRLLLVTKEAAKMKVPLGIKLVRGAYMSSESKLASSLGFESPIHNSIQDTHFCYNT 383
           KDA DRL L TK A KM VPLG+KLVRGAYMSSE +LASSLG+ESPIHN+IQDTH  +N 
Sbjct: 332 KDAGDRLFLATKAAEKMGVPLGVKLVRGAYMSSERELASSLGYESPIHNTIQDTHSSFND 391

Query: 384 CASFLLDNIAKGSSGAILATHNVESGKLAASKAYEIGIGKMKQKLEFAQLYGMSEALSFG 443
           CASF+L+ +A GS   +LATHNV+SGKLAA+KA+++GIG++ QKLEFAQLYGM+++LS+G
Sbjct: 392 CASFMLERLANGSDALVLATHNVQSGKLAAAKAHDLGIGRVNQKLEFAQLYGMADSLSYG 451

Query: 444 LRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSASNLDRQLMRKELGRRM 487
           L NAGFQVSKYMPFGP+D+VMPYLLRRAEENRG+LS+S LDR+LMRKEL RR+
Sbjct: 452 LGNAGFQVSKYMPFGPIDVVMPYLLRRAEENRGMLSSSTLDRELMRKELKRRL 504

BLAST of Cla016474 vs. NCBI nr
Match: gi|657971712|ref|XP_008377648.1| (PREDICTED: proline dehydrogenase 2, mitochondrial-like [Malus domestica])

HSP 1 Score: 631.7 bits (1628), Expect = 1.1e-177
Identity = 309/493 (62.68%), Postives = 388/493 (78.70%), Query Frame = 1

Query: 1   MARSIVSTQSKISKKVFFYAFNRSLNTAASS------PAAAFTAAATSPPPPINLTDSPA 60
           MA  ++   SK+ K +      R LN+A++S      P   F+         I  +D+ A
Sbjct: 1   MANRVIPAASKLLKTI------RPLNSASTSTISGISPPIGFSEKTPQATTAITPSDNAA 60

Query: 61  TPTVDFTDTRTLFGSLSTSELLRAAANLHVAAIGPVVDVGMWVMNSKLMDVEVIRDVVLG 120
           T   +F D   LF S+STS LLR A NLH+  + P+VD+GMWVM S+LM   +I+D +LG
Sbjct: 61  TNLSNFDDADKLFASVSTSRLLRTALNLHLVGMEPMVDIGMWVMRSRLMQTPLIKDAILG 120

Query: 121 TVKHTFYRHFCAGEDDAAVAKTVRRLHDAGLRSMLDYALEYADDEASCDRNLDGFLRTIE 180
           T++ T Y HFCAGE+ AA  K+V  L++AGLR ML YALEYA D  +CDRNL GFL T+E
Sbjct: 121 TIRSTIYDHFCAGENPAATGKSVLELNEAGLRGMLVYALEYAGDNEACDRNLQGFLDTVE 180

Query: 181 ATRSLPPGSASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLKTLPIFSESSPL 240
           +T+SLPP S SF++ K++AICP+ LL+RVSDLLRWQQ++PSFNLPWK  T PIFSESSPL
Sbjct: 181 STKSLPPSSVSFIILKITAICPMNLLQRVSDLLRWQQQDPSFNLPWKRDTFPIFSESSPL 240

Query: 241 YHTLEQPKHLTHEEEKSLQMSHERLMKICQSCVEANVPLAIDAEHTKVQPAIDYFTYSAA 300
           YHTL++P+ LT EEE+ L++ H+RL+K+ Q+CVE NVPL+IDAE+T +QPAIDY TYS+A
Sbjct: 241 YHTLKKPEPLTPEEERDLELVHQRLLKLSQNCVEVNVPLSIDAEYTSIQPAIDYLTYSSA 300

Query: 301 IIHNKDHNPIVYGTIQAYLKDAKDRLLLVTKEAAKMKVPLGIKLVRGAYMSSESKLASSL 360
           II+NKD NPIVYGTIQAYLKDAK+RLLL TK A KM VP+G K+VRGAYMSSE KLASSL
Sbjct: 301 IIYNKDDNPIVYGTIQAYLKDAKERLLLATKAADKMGVPMGFKVVRGAYMSSEGKLASSL 360

Query: 361 GFESPIHNSIQDTHFCYNTCASFLLDNIAKGSSGAILATHNVESGKLAASKAYEIGIGKM 420
           G++SPIH+ I+DTH CYN CASF+L+ IA GS   +LATHNVESG+LA +KA+EIG+ K+
Sbjct: 361 GYKSPIHDCIEDTHVCYNDCASFMLERIANGSDAVVLATHNVESGRLAMAKAHEIGVRKV 420

Query: 421 KQKLEFAQLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSASNLD 480
            QKLEFAQLYGM+E+LSFGL+NAGFQVSKYMPFGP+ +V+PYLLRRAEENRGLL+AS+LD
Sbjct: 421 HQKLEFAQLYGMAESLSFGLKNAGFQVSKYMPFGPIQLVLPYLLRRAEENRGLLNASSLD 480

Query: 481 RQLMRKELGRRMK 488
           +QL R+EL RR+K
Sbjct: 481 KQLTREELMRRLK 487

BLAST of Cla016474 vs. NCBI nr
Match: gi|645248575|ref|XP_008230359.1| (PREDICTED: proline dehydrogenase 2, mitochondrial [Prunus mume])

HSP 1 Score: 627.9 bits (1618), Expect = 1.5e-176
Identity = 314/500 (62.80%), Postives = 387/500 (77.40%), Query Frame = 1

Query: 1   MARSIVSTQSKISKKVFFYAFNRSLNTAASSPAAAFTAAATSPP-----PPINLTDSPAT 60
           MA  ++   SK+ K +      R LN+AASS     T +A SPP      P+      A 
Sbjct: 1   MANRVIPAASKLLKTL------RPLNSAASST----TISAISPPIGYAEKPLQSPSGHAI 60

Query: 61  PTVD--------FTDTRTLFGSLSTSELLRAAANLHVAAIGPVVDVGMWVMNSKLMDVEV 120
              D          D   LF S+ST  LLRAA NLH  A  P+VD+GMWVM S+LMD  +
Sbjct: 61  TAADTITDNIFNLDDVDKLFASVSTPTLLRAALNLHAVAFEPLVDIGMWVMKSRLMDTPL 120

Query: 121 IRDVVLGTVKHTFYRHFCAGEDDAAVAKTVRRLHDAGLRSMLDYALEYADDEASCDRNLD 180
           IRDV++G ++ TFY HFCAGE+  A  ++V  L++AGLR ML YALEYA D  +CDRNL 
Sbjct: 121 IRDVIMGIIRSTFYEHFCAGENAMAAGRSVLELNEAGLRGMLVYALEYAGDNDACDRNLQ 180

Query: 181 GFLRTIEATRSLPPGSASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLKTLPI 240
           GFL T+EAT+SLPP S SF++ K++AICP+R+LERVSDLLRWQ K+PSF LPWKL TLPI
Sbjct: 181 GFLDTVEATKSLPPSSVSFIIMKITAICPMRVLERVSDLLRWQHKDPSFKLPWKLDTLPI 240

Query: 241 FSESSPLYHTLEQPKHLTHEEEKSLQMSHERLMKICQSCVEANVPLAIDAEHTKVQPAID 300
           FS++SPLYHTLE+P+ LT +EE+ L++ H+RL+K+ Q C EANVPL++DAE++ VQPAID
Sbjct: 241 FSDNSPLYHTLEKPEPLTPDEERDLELVHQRLLKLSQKCSEANVPLSVDAEYSSVQPAID 300

Query: 301 YFTYSAAIIHNKDHNPIVYGTIQAYLKDAKDRLLLVTKEAAKMKVPLGIKLVRGAYMSSE 360
           Y TYS+AII+NKD NPIVYGTIQAYLKDAK+RLLL TK A  M +P+G K+VRGAYMSSE
Sbjct: 301 YLTYSSAIIYNKDDNPIVYGTIQAYLKDAKERLLLATKAADDMGIPMGFKVVRGAYMSSE 360

Query: 361 SKLASSLGFESPIHNSIQDTHFCYNTCASFLLDNIAKGSSGAILATHNVESGKLAASKAY 420
           +K+ASSLG++SPIH+SI+DTH CYN C SF+L+ IA GS G +LATHNVESGKLA +KA+
Sbjct: 361 AKVASSLGYKSPIHDSIEDTHACYNDCTSFMLEKIANGSGGVVLATHNVESGKLAVAKAH 420

Query: 421 EIGIGKMKQKLEFAQLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGL 480
           EIGIGK++QKLEFAQLYGM+E+LSFGLRNAGFQVSKYMPFGP+++V+PYLLRRAEENRGL
Sbjct: 421 EIGIGKVRQKLEFAQLYGMAESLSFGLRNAGFQVSKYMPFGPIELVLPYLLRRAEENRGL 480

Query: 481 LSASNLDRQLMRKELGRRMK 488
           LSAS  DRQ  R+ L RR+K
Sbjct: 481 LSASTQDRQRTREVLKRRLK 490