Cla011134.1 (mRNA) Watermelon (97103) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cla011134
Type	mRNA
Organism	Citrullus. lanatus (Watermelon (97103) v1)
Description	Pectinesterase/pectinesterase inhibitor 3 (AHRD V1 **** PME3_ARATH); contains Interpro domain(s) IPR006501 Pectinesterase inhibitor
Location	Chr3 : 25581032 .. 25581631 (+)
Sequence length	600

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACTTTCCACTCTCTCCGCCTCCTCCTCCTCCTCTCACTCTGTCTCTCTGCCGCCGCCCACGACGGCGCTGCCGTCTCCCAAGATCTGATCCGCTCCTCCTGCCTCCAAGCAAGCTACCCAAATCTCTGCGTCAGAACCCTCTCCTCCTACGGCGGCGCCGTCAACACATCACAGGATCTTGCCCAAGCCGCCATCTCCGTCAGCCTCTCCCTCGCCCGCGACCTCTCCCGGTATCTCTCCGACGCCCGCCGGCAGGCGTCGAGGCGGCAGCGCGCGGCGGTGAATGACTGCGTGGATCAGATTGGGGACTCTGTTGAGGAGCTGAGCAACACTTTGAGCGTCCTCCGGCACCTCCCCTGCGGCAGCGACCGGCGGGCTTTCCGGCTGCAGATGGGGAATGCGAAGACGTGGGTTAGCGCTGCCTTGACGAATGAATACACGTGTCTCGATGGGTTTAAAGAAGTGGAAGGGGAAGTGAAATTGGATGTGAAGAAGAGGATTTCGAAGGTGGCTAAAGTTACTAGTAATGCTCTGTTTTTTATTAATCGCCTCGACGGTGGAAACCCCGCCGGAAGGAAGAAGTTGGCCGCTCCTTGA

mRNA sequence

ATGACTTTCCACTCTCTCCGCCTCCTCCTCCTCCTCTCACTCTGTCTCTCTGCCGCCGCCCACGACGGCGCTGCCGTCTCCCAAGATCTGATCCGCTCCTCCTGCCTCCAAGCAAGCTACCCAAATCTCTGCGTCAGAACCCTCTCCTCCTACGGCGGCGCCGTCAACACATCACAGGATCTTGCCCAAGCCGCCATCTCCGTCAGCCTCTCCCTCGCCCGCGACCTCTCCCGGTATCTCTCCGACGCCCGCCGGCAGGCGTCGAGGCGGCAGCGCGCGGCGGTGAATGACTGCGTGGATCAGATTGGGGACTCTGTTGAGGAGCTGAGCAACACTTTGAGCGTCCTCCGGCACCTCCCCTGCGGCAGCGACCGGCGGGCTTTCCGGCTGCAGATGGGGAATGCGAAGACGTGGGTTAGCGCTGCCTTGACGAATGAATACACGTGTCTCGATGGGTTTAAAGAAGTGGAAGGGGAAGTGAAATTGGATGTGAAGAAGAGGATTTCGAAGGTGGCTAAAGTTACTAGTAATGCTCTGTTTTTTATTAATCGCCTCGACGGTGGAAACCCCGCCGGAAGGAAGAAGTTGGCCGCTCCTTGA

Coding sequence (CDS)

ATGACTTTCCACTCTCTCCGCCTCCTCCTCCTCCTCTCACTCTGTCTCTCTGCCGCCGCCCACGACGGCGCTGCCGTCTCCCAAGATCTGATCCGCTCCTCCTGCCTCCAAGCAAGCTACCCAAATCTCTGCGTCAGAACCCTCTCCTCCTACGGCGGCGCCGTCAACACATCACAGGATCTTGCCCAAGCCGCCATCTCCGTCAGCCTCTCCCTCGCCCGCGACCTCTCCCGGTATCTCTCCGACGCCCGCCGGCAGGCGTCGAGGCGGCAGCGCGCGGCGGTGAATGACTGCGTGGATCAGATTGGGGACTCTGTTGAGGAGCTGAGCAACACTTTGAGCGTCCTCCGGCACCTCCCCTGCGGCAGCGACCGGCGGGCTTTCCGGCTGCAGATGGGGAATGCGAAGACGTGGGTTAGCGCTGCCTTGACGAATGAATACACGTGTCTCGATGGGTTTAAAGAAGTGGAAGGGGAAGTGAAATTGGATGTGAAGAAGAGGATTTCGAAGGTGGCTAAAGTTACTAGTAATGCTCTGTTTTTTATTAATCGCCTCGACGGTGGAAACCCCGCCGGAAGGAAGAAGTTGGCCGCTCCTTGA

Protein sequence

MTFHSLRLLLLLSLCLSAAAHDGAAVSQDLIRSSCLQASYPNLCVRTLSSYGGAVNTSQDLAQAAISVSLSLARDLSRYLSDARRQASRRQRAAVNDCVDQIGDSVEELSNTLSVLRHLPCGSDRRAFRLQMGNAKTWVSAALTNEYTCLDGFKEVEGEVKLDVKKRISKVAKVTSNALFFINRLDGGNPAGRKKLAAP

Homology

[Close]

BLAST of Cla011134 vs. Swiss-Prot
Match: 21KD_DAUCA (21 kDa protein OS=Daucus carota PE=2 SV=1)

HSP 1 Score: 113.2 bits (282), Expect = 3.2e-24
Identity = 65/181 (35.91%), Postives = 107/181 (59.12%), Query Frame = 1


Query: 10  LLLSLCLSAAAHDGAAVSQDLIRSSCLQASYPNLCVRTLSSYGGAV-NTSQDLAQAAISV 69
           L+ S  L   A   AA +   I++SC   +YP +C ++LS+Y   + N  Q+LA  A+ V
Sbjct: 8   LVFSALLVILAAASAAPANQFIKTSCTLTTYPAVCEQSLSAYAKTIQNNPQELASTALQV 67

Query: 70  SLSLARDLSRYLSDARR--QASRRQRAAVNDCVDQIGDSVEELSNTLSVLRHLPC--GSD 129
           SL+  +    ++    +      RQ AA++DC++++ DS++ +S +   +++L    G+D
Sbjct: 68  SLTRTQQAQTFMKRLNKFKGLKARQYAAIHDCLEEVEDSLDRVSRSCDEMKNLSHAKGND 127

Query: 130 RRAFRLQMGNAKTWVSAALTNEYTCLDGF--KEVEGEVKLDVKKRISKVAKVTSNALFFI 184
              F  +M N +TWVSAALT+E TC+DGF  K ++G++K  V+ ++  VA+VTSNAL  +
Sbjct: 128 ---FTFRMSNVETWVSAALTDETTCMDGFAGKGMDGKIKESVRAQVVAVARVTSNALALV 185

[Close]

BLAST of Cla011134 vs. Swiss-Prot
Match: PME3_ARATH (Pectinesterase/pectinesterase inhibitor 3 OS=Arabidopsis thaliana GN=PME3 PE=2 SV=2)

HSP 1 Score: 82.8 bits (203), Expect = 4.6e-15
Identity = 50/165 (30.30%), Postives = 90/165 (54.55%), Query Frame = 1


Query: 27  SQDLIRSSCLQASYPNLCVRTLSSYGGAVNTSQ-DLAQAAISVSLSLARD---LSRYLSD 86
           S  ++RSSC    YP LC+  + + GG   TSQ D+ +A+++++++         + L  
Sbjct: 55  SHAVLRSSCSSTRYPELCISAVVTAGGVELTSQKDVIEASVNLTITAVEHNYFTVKKLIK 114

Query: 87  ARRQASRRQRAAVNDCVDQIGDSVEELSNTLSVLRHLPCGSDRRAFRLQMGNAKTWVSAA 146
            R+  + R++ A++DC++ I ++++EL  T+  L   P    ++  R   G+ KT +S+A
Sbjct: 115 KRKGLTPREKTALHDCLETIDETLDELHETVEDLHLYPT---KKTLREHAGDLKTLISSA 174

Query: 147 LTNEYTCLDGFK--EVEGEVKLDVKKRISKVAKVTSNALFFINRL 186
           +TN+ TCLDGF   + + +V+  + K    V  + SNAL  I  +
Sbjct: 175 ITNQETCLDGFSHDDADKQVRKALLKGQIHVEHMCSNALAMIKNM 216

[Close]

BLAST of Cla011134 vs. Swiss-Prot
Match: PMEU1_SOLLC (Pectinesterase/pectinesterase inhibitor U1 OS=Solanum lycopersicum GN=PMEU1 PE=2 SV=1)

HSP 1 Score: 77.8 bits (190), Expect = 1.5e-13
Identity = 49/194 (25.26%), Postives = 103/194 (53.09%), Query Frame = 1


Query: 14  LCLSAAAHDGAAVSQDLIRSSCLQASYPNLC---VRTLSSYGGAVNTSQDLAQAAISVSL 73
           + +S++AH        +++S+C    +P LC   +  +S +   V + +D+ + ++++++
Sbjct: 56  MAISSSAHA-------IVKSACSNTLHPELCYSAIVNVSDFSKKVTSQKDVIELSLNITV 115

Query: 74  SLARD---LSRYLSDARRQASRRQRAAVNDCVDQIGDSVEELSNTLSVLRHLPCGSDRRA 133
              R      + L   R+  + R++ A++DC++ + ++++EL   +  L   P   ++++
Sbjct: 116 KAVRRNYYAVKELIKTRKGLTPREKVALHDCLETMDETLDELHTAVEDLELYP---NKKS 175

Query: 134 FRLQMGNAKTWVSAALTNEYTCLDGFK--EVEGEVKLDVKKRISKVAKVTSNALFFINRL 193
            +  + + KT +S+A+TN+ TCLDGF   E + +V+  + K    V K+ SNAL  I  +
Sbjct: 176 LKEHVEDLKTLISSAITNQETCLDGFSHDEADKKVRKVLLKGQKHVEKMCSNALAMICNM 235

Query: 194 DGGNPAGRKKLAAP 200
              + A   KL+AP
Sbjct: 236 TDTDIANEMKLSAP 239

[Close]

BLAST of Cla011134 vs. Swiss-Prot
Match: PME2_ARATH (Pectinesterase 2 OS=Arabidopsis thaliana GN=PME2 PE=2 SV=2)

HSP 1 Score: 69.3 bits (168), Expect = 5.3e-11
Identity = 45/168 (26.79%), Postives = 88/168 (52.38%), Query Frame = 1


Query: 24  AAVSQDLIRSSCLQASYPNLCVRTLSSYGGAVNTSQ-DLAQAAISVSLSLARD---LSRY 83
           ++ S  +++S C    YP LC   +++ GG   TSQ ++ +A+++++    +      + 
Sbjct: 55  SSTSHAILKSVCSSTLYPELCFSAVAATGGKELTSQKEVIEASLNLTTKAVKHNYFAVKK 114

Query: 84  LSDARRQASRRQRAAVNDCVDQIGDSVEELSNTLSVLRHLPCGSDRRAFRLQMGNAKTWV 143
           L   R+  + R+  A++DC++ I ++++EL   +  L   P    +++ R    + KT +
Sbjct: 115 LIAKRKGLTPREVTALHDCLETIDETLDELHVAVEDLHQYP---KQKSLRKHADDLKTLI 174

Query: 144 SAALTNEYTCLDGFK--EVEGEVKLDVKKRISKVAKVTSNALFFINRL 186
           S+A+TN+ TCLDGF   + + +V+  + K    V  + SNAL  I  +
Sbjct: 175 SSAITNQGTCLDGFSYDDADRKVRKALLKGQVHVEHMCSNALAMIKNM 219

[Close]

BLAST of Cla011134 vs. Swiss-Prot
Match: PME32_ARATH (Probable pectinesterase/pectinesterase inhibitor 32 OS=Arabidopsis thaliana GN=PME32 PE=2 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 5.3e-11
Identity = 57/175 (32.57%), Postives = 85/175 (48.57%), Query Frame = 1


Query: 9   LLLLSLCLSAAAHDGAAVSQDLIRSSCLQASYPNLCVRTLSSYGGAVNTSQDLAQAAISV 68
           L+++SLC   +AH  A  S DL++  CL+   P L       +  A  T  D    A+++
Sbjct: 16  LIIISLC---SAHKEAFSSTDLVQMECLRV--PPL------EFAEAAKTVVDAITKAVAI 75

Query: 69  SLSLARDLSRYLSDARRQASRRQRAAVNDCVDQIGDSVEELSNTLSVLRHLPCGSDRRAF 128
                  +S++  D +   SR   A V DCVD +  + EELS  +S  +  P G D    
Sbjct: 76  -------VSKF--DKKAGKSRVSNAIV-DCVDLLDSAAEELSWIISASQS-PNGKDNSTG 135

Query: 129 RLQMGNAKTWVSAALTNEYTCLDGFKEVEGEVKLDVKKRISKVAKVTSNALFFIN 184
            +   + +TW+SAAL+N+ TCLDGF+   G +K  V   +SKV     N L  ++
Sbjct: 136 DVG-SDLRTWISAALSNQDTCLDGFEGTNGIIKKIVAGGLSKVGTTVRNLLTMVH 167

[Close]

BLAST of Cla011134 vs. TrEMBL
Match: A0A0A0LWR8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G597770 PE=4 SV=1)

HSP 1 Score: 301.2 bits (770), Expect = 9.3e-79
Identity = 159/196 (81.12%), Postives = 170/196 (86.73%), Query Frame = 1


Query: 1   MTFHSLRLLLLLSLCLSAAAHDGAAVSQDLIRSSCLQASYPNLCVRTLSSYGGAVNTSQD 60
           MT  SL LLLL SL LSAA     A+SQDLI SSCLQASYP LC+RTLSSY GAV T +D
Sbjct: 28  MTVLSL-LLLLFSLSLSAAHGGRGAISQDLIHSSCLQASYPTLCIRTLSSYAGAVKTPRD 87

Query: 61  LAQAAISVSLSLARDLSRYLSDARRQASRRQRAAVNDCVDQIGDSVEELSNTLSVLRHLP 120
           LAQA ISVSLSLA++LS YLSD+ RQASR+QRAAV+DCVDQIGDSVEELSNTL VLRHLP
Sbjct: 88  LAQATISVSLSLAQNLSEYLSDSLRQASRQQRAAVDDCVDQIGDSVEELSNTLGVLRHLP 147

Query: 121 CGSDRRAFRLQMGNAKTWVSAALTNEYTCLDGFKEVEGEVKLDVKKRISKVAKVTSNALF 180
           CG DRR FRL+MGNAKTWVSAALTNE TCLDGFKEV+GEVKLDVK+RI KVAKVTSNALF
Sbjct: 148 CGDDRRKFRLEMGNAKTWVSAALTNEETCLDGFKEVDGEVKLDVKRRILKVAKVTSNALF 207

Query: 181 FINRLDGGNPAGRKKL 197
            INRLDGGNP GRK L
Sbjct: 208 MINRLDGGNPTGRKML 222

[Close]

BLAST of Cla011134 vs. TrEMBL
Match: A0A061GSW0_THECC (Plant invertase/pectin methylesterase inhibitor superfamily protein OS=Theobroma cacao GN=TCM_037478 PE=4 SV=1)

HSP 1 Score: 203.8 bits (517), Expect = 2.0e-49
Identity = 109/186 (58.60%), Postives = 135/186 (72.58%), Query Frame = 1


Query: 1   MTFHSLRLLLLLSLCLSAAAHDGAAVSQDLIRSSCLQASYPNLCVRTLSSYGGAVNTSQD 60
           MT     LL    LCLS+AA       +DL+RSSC+ ASYP+LC+RTLSSY G  NT +D
Sbjct: 1   MTTIPFFLLTTSLLCLSSAAASKQDAPRDLVRSSCVHASYPSLCLRTLSSYSGPANTPRD 60

Query: 61  LAQAAISVSLSLARDLSRYLSDARRQASRRQRAAVNDCVDQIGDSVEELSNTLSVLRHLP 120
           LAQAA+ VSLS AR +S YL+ +    S+R+R A++DCV+QI +SVEELS TL  L+HL 
Sbjct: 61  LAQAAVKVSLSRARKVSNYLTTSVTGKSKRERGALSDCVEQIAESVEELSRTLGELKHL- 120

Query: 121 CGSDRRAFRLQMGNAKTWVSAALTNEYTCLDGFKEVEGEVKLDVKKRISKVAKVTSNALF 180
                  F  QM NA+TWVSAALTNE TCLDGF+ V+G+VK DVK++I+ VAKVTSNAL+
Sbjct: 121 ---RGETFEFQMSNAQTWVSAALTNEDTCLDGFEGVDGKVKSDVKRKITNVAKVTSNALY 180

Query: 181 FINRLD 187
            INRLD
Sbjct: 181 MINRLD 182

[Close]

BLAST of Cla011134 vs. TrEMBL
Match: B9RR57_RICCO (21 kDa protein, putative OS=Ricinus communis GN=RCOM_0710110 PE=4 SV=1)

HSP 1 Score: 199.5 bits (506), Expect = 3.8e-48
Identity = 106/179 (59.22%), Postives = 133/179 (74.30%), Query Frame = 1


Query: 9   LLLLSLCLSAA-AHDGAAVSQDLIRSSCLQASYPNLCVRTLSSYGGAVNTSQDLAQAAIS 68
           +L LSLC +AA  H   A  QDL+ SSCL ASYPNLC+RTLSSY G  NT  DLA AA+ 
Sbjct: 73  ILFLSLCSAAAKTHSAGAAPQDLVHSSCLHASYPNLCMRTLSSYSGPANTPHDLALAAVE 132

Query: 69  VSLSLARDLSRYLSD-ARRQASRRQRAAVNDCVDQIGDSVEELSNTLSVLRHLPCGSDRR 128
           VSL  A  +S+YLS  +    +R++R A++DCV+QI DSV+ELSNTL+ L+HL       
Sbjct: 133 VSLGRASKVSKYLSSLSSGLKTRKERVALSDCVEQISDSVDELSNTLNELKHLR----GE 192

Query: 129 AFRLQMGNAKTWVSAALTNEYTCLDGFKEVEGEVKLDVKKRISKVAKVTSNALFFINRL 186
            FR QM NA+TWVSAALTNE TCLDGF++V+ +VK DVK++I+ VA+VTSNAL+ INRL
Sbjct: 193 TFRWQMNNAQTWVSAALTNEETCLDGFEQVQRKVKCDVKRKITNVARVTSNALYMINRL 247

[Close]

BLAST of Cla011134 vs. TrEMBL
Match: U5GAM6_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0006s14010g PE=4 SV=1)

HSP 1 Score: 194.9 bits (494), Expect = 9.3e-47
Identity = 108/196 (55.10%), Postives = 136/196 (69.39%), Query Frame = 1


Query: 5   SLRLLLLLSLCLSAAAHDGAAVS----QDLIRSSCLQASYPNLCVRTLSSYGGAVNTSQD 64
           SL  LL + +  S + +  AA S    +DL+RSSC+ ASYPNLC+RTLSSY G  NT +D
Sbjct: 12  SLFNLLFIFISFSTSPYSAAAKSHNAPRDLVRSSCVHASYPNLCLRTLSSYAGPANTPRD 71

Query: 65  LAQAAISVSLSLARDLSRYLSDARRQASRRQRAAVNDCVDQIGDSVEELSNTLSVLRHLP 124
           LAQAA+ VS++ AR +S YLS       +R+R A++DC++QI DSV+ELS TL  L+HL 
Sbjct: 72  LAQAAVKVSIARARKVSNYLSTLSGLKKKRERVALSDCIEQIYDSVDELSKTLGELKHL- 131

Query: 125 CGSDRRAFRLQMGNAKTWVSAALTNEYTCLDGFKEVEGEVKLDVKKRISKVAKVTSNALF 184
                  F  QM NA+TWVSAALTNE TCLDGF EVE + K DVK++I+ VA+VTSNAL+
Sbjct: 132 ---REETFGWQMSNAQTWVSAALTNEDTCLDGFHEVESKAKDDVKRKITNVARVTSNALY 191

Query: 185 FINRLDGGNPAGRKKL 197
            INRLD     GR KL
Sbjct: 192 MINRLD--ESRGRPKL 201

[Close]

BLAST of Cla011134 vs. TrEMBL
Match: F6HGM6_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_11s0016g00590 PE=4 SV=1)

HSP 1 Score: 193.4 bits (490), Expect = 2.7e-46
Identity = 99/162 (61.11%), Postives = 128/162 (79.01%), Query Frame = 1


Query: 27  SQDLIRSSCLQASYPNLCVRTLSSYGGAVNTSQDLAQAAISVSLSLARDLSRYLSDARRQ 86
           ++DL+ SSC+ ASYPN+C+RTLSSY G   T +DLAQAA+ VSLS A+ +S YL+    Q
Sbjct: 21  AEDLVHSSCIHASYPNICLRTLSSYSGPAKTPRDLAQAAVKVSLSRAQRVSTYLNGVAGQ 80

Query: 87  --ASRRQRAAVNDCVDQIGDSVEELSNTLSVLRHLPCGSDRRAFRLQMGNAKTWVSAALT 146
             AS+R++ A++DCV+QI D+VEELSNTL  L+HL  G+    FR QM NA+TWVSAALT
Sbjct: 81  GKASKREQVALSDCVEQISDTVEELSNTLKELKHLRPGT----FRWQMSNAETWVSAALT 140

Query: 147 NEYTCLDGFKEVEGEVKLDVKKRISKVAKVTSNALFFINRLD 187
           NE TCLDGF+ V+G+VK DVK++I+ VA+VTSNAL+ INRLD
Sbjct: 141 NEDTCLDGFQGVDGKVKADVKRKITNVARVTSNALYLINRLD 178

[Close]

BLAST of Cla011134 vs. NCBI nr
Match: gi|449452771|ref|XP_004144132.1| (PREDICTED: 21 kDa protein [Cucumis sativus])

HSP 1 Score: 301.2 bits (770), Expect = 1.3e-78
Identity = 159/196 (81.12%), Postives = 170/196 (86.73%), Query Frame = 1


Query: 1   MTFHSLRLLLLLSLCLSAAAHDGAAVSQDLIRSSCLQASYPNLCVRTLSSYGGAVNTSQD 60
           MT  SL LLLL SL LSAA     A+SQDLI SSCLQASYP LC+RTLSSY GAV T +D
Sbjct: 1   MTVLSL-LLLLFSLSLSAAHGGRGAISQDLIHSSCLQASYPTLCIRTLSSYAGAVKTPRD 60

Query: 61  LAQAAISVSLSLARDLSRYLSDARRQASRRQRAAVNDCVDQIGDSVEELSNTLSVLRHLP 120
           LAQA ISVSLSLA++LS YLSD+ RQASR+QRAAV+DCVDQIGDSVEELSNTL VLRHLP
Sbjct: 61  LAQATISVSLSLAQNLSEYLSDSLRQASRQQRAAVDDCVDQIGDSVEELSNTLGVLRHLP 120

Query: 121 CGSDRRAFRLQMGNAKTWVSAALTNEYTCLDGFKEVEGEVKLDVKKRISKVAKVTSNALF 180
           CG DRR FRL+MGNAKTWVSAALTNE TCLDGFKEV+GEVKLDVK+RI KVAKVTSNALF
Sbjct: 121 CGDDRRKFRLEMGNAKTWVSAALTNEETCLDGFKEVDGEVKLDVKRRILKVAKVTSNALF 180

Query: 181 FINRLDGGNPAGRKKL 197
            INRLDGGNP GRK L
Sbjct: 181 MINRLDGGNPTGRKML 195

[Close]

BLAST of Cla011134 vs. NCBI nr
Match: gi|700211234|gb|KGN66330.1| (hypothetical protein Csa_1G597770 [Cucumis sativus])

HSP 1 Score: 301.2 bits (770), Expect = 1.3e-78
Identity = 159/196 (81.12%), Postives = 170/196 (86.73%), Query Frame = 1


Query: 1   MTFHSLRLLLLLSLCLSAAAHDGAAVSQDLIRSSCLQASYPNLCVRTLSSYGGAVNTSQD 60
           MT  SL LLLL SL LSAA     A+SQDLI SSCLQASYP LC+RTLSSY GAV T +D
Sbjct: 28  MTVLSL-LLLLFSLSLSAAHGGRGAISQDLIHSSCLQASYPTLCIRTLSSYAGAVKTPRD 87

Query: 61  LAQAAISVSLSLARDLSRYLSDARRQASRRQRAAVNDCVDQIGDSVEELSNTLSVLRHLP 120
           LAQA ISVSLSLA++LS YLSD+ RQASR+QRAAV+DCVDQIGDSVEELSNTL VLRHLP
Sbjct: 88  LAQATISVSLSLAQNLSEYLSDSLRQASRQQRAAVDDCVDQIGDSVEELSNTLGVLRHLP 147

Query: 121 CGSDRRAFRLQMGNAKTWVSAALTNEYTCLDGFKEVEGEVKLDVKKRISKVAKVTSNALF 180
           CG DRR FRL+MGNAKTWVSAALTNE TCLDGFKEV+GEVKLDVK+RI KVAKVTSNALF
Sbjct: 148 CGDDRRKFRLEMGNAKTWVSAALTNEETCLDGFKEVDGEVKLDVKRRILKVAKVTSNALF 207

Query: 181 FINRLDGGNPAGRKKL 197
            INRLDGGNP GRK L
Sbjct: 208 MINRLDGGNPTGRKML 222

[Close]

BLAST of Cla011134 vs. NCBI nr
Match: gi|659100173|ref|XP_008450965.1| (PREDICTED: 21 kDa protein [Cucumis melo])

HSP 1 Score: 296.6 bits (758), Expect = 3.3e-77
Identity = 154/194 (79.38%), Postives = 168/194 (86.60%), Query Frame = 1


Query: 1   MTFHSLRLLLLLSLCLSAAAHDGAAVSQDLIRSSCLQASYPNLCVRTLSSYGGAVNTSQD 60
           MT  SL LLLLLSL LSAA   G AVSQDLIRSSCLQA YP LC+RTLSSY G+V T +D
Sbjct: 1   MTVLSLLLLLLLSLSLSAAHGGGGAVSQDLIRSSCLQARYPTLCIRTLSSYAGSVKTPRD 60

Query: 61  LAQAAISVSLSLARDLSRYLSDARRQASRRQRAAVNDCVDQIGDSVEELSNTLSVLRHLP 120
           LAQ  ISVSLSLA++LS YLSD+ R+ASR+QRAAV+DCVDQIGDSVEELSNTL VLRHLP
Sbjct: 61  LAQVTISVSLSLAQNLSEYLSDSLRKASRQQRAAVDDCVDQIGDSVEELSNTLGVLRHLP 120

Query: 121 CGSDRRAFRLQMGNAKTWVSAALTNEYTCLDGFKEVEGEVKLDVKKRISKVAKVTSNALF 180
           CG DRR FRL+MGNAKTWVSAALTNE TCLDGFKEV+GEVKLDVK+RI KVAKVTSNALF
Sbjct: 121 CGDDRRKFRLEMGNAKTWVSAALTNEETCLDGFKEVDGEVKLDVKRRIVKVAKVTSNALF 180

Query: 181 FINRLDGGNPAGRK 195
            INRLD GN  G++
Sbjct: 181 MINRLDSGNSTGKE 194

[Close]

BLAST of Cla011134 vs. NCBI nr
Match: gi|720018304|ref|XP_010261745.1| (PREDICTED: 21 kDa protein-like [Nelumbo nucifera])

HSP 1 Score: 206.8 bits (525), Expect = 3.4e-50
Identity = 110/191 (57.59%), Postives = 141/191 (73.82%), Query Frame = 1


Query: 3   FHSLRLLLLLSLCLSAAAHDGAAVS-----QDLIRSSCLQASYPNLCVRTLSSYGGAVNT 62
           F S    L+  LC S+A   GAA +     QDL+RSSC+ ASYPNLC+RTLSSY G+ NT
Sbjct: 5   FFSFITFLVFFLCFSSATFYGAATTKSGAKQDLVRSSCIHASYPNLCLRTLSSYAGSANT 64

Query: 63  SQDLAQAAISVSLSLARDLSRYLSDARR--QASRRQRAAVNDCVDQIGDSVEELSNTLSV 122
             DLAQAA+++SL  AR +S YL+  ++    S+RQ+ AV+DCV+Q+ DSV+EL  TLS 
Sbjct: 65  PNDLAQAAVTISLGRARSVSLYLARLQKLHNGSKRQQGAVSDCVEQLSDSVDELQQTLSE 124

Query: 123 LRHLPCGSDRRAFRLQMGNAKTWVSAALTNEYTCLDGFKEVEGEVKLDVKKRISKVAKVT 182
           LRHL  G+    FR QM NA+TWVSAALTNE TCLDGF+ V+G+VK DVK++I+ VA++T
Sbjct: 125 LRHLRRGT----FREQMSNAQTWVSAALTNEDTCLDGFQNVDGKVKSDVKQKINNVARIT 184

Query: 183 SNALFFINRLD 187
           SNAL+FIN LD
Sbjct: 185 SNALYFINLLD 191

[Close]

BLAST of Cla011134 vs. NCBI nr
Match: gi|590574990|ref|XP_007012560.1| (Plant invertase/pectin methylesterase inhibitor superfamily protein [Theobroma cacao])

HSP 1 Score: 203.8 bits (517), Expect = 2.9e-49
Identity = 109/186 (58.60%), Postives = 135/186 (72.58%), Query Frame = 1


Query: 1   MTFHSLRLLLLLSLCLSAAAHDGAAVSQDLIRSSCLQASYPNLCVRTLSSYGGAVNTSQD 60
           MT     LL    LCLS+AA       +DL+RSSC+ ASYP+LC+RTLSSY G  NT +D
Sbjct: 1   MTTIPFFLLTTSLLCLSSAAASKQDAPRDLVRSSCVHASYPSLCLRTLSSYSGPANTPRD 60

Query: 61  LAQAAISVSLSLARDLSRYLSDARRQASRRQRAAVNDCVDQIGDSVEELSNTLSVLRHLP 120
           LAQAA+ VSLS AR +S YL+ +    S+R+R A++DCV+QI +SVEELS TL  L+HL 
Sbjct: 61  LAQAAVKVSLSRARKVSNYLTTSVTGKSKRERGALSDCVEQIAESVEELSRTLGELKHL- 120

Query: 121 CGSDRRAFRLQMGNAKTWVSAALTNEYTCLDGFKEVEGEVKLDVKKRISKVAKVTSNALF 180
                  F  QM NA+TWVSAALTNE TCLDGF+ V+G+VK DVK++I+ VAKVTSNAL+
Sbjct: 121 ---RGETFEFQMSNAQTWVSAALTNEDTCLDGFEGVDGKVKSDVKRKITNVAKVTSNALY 180

Query: 181 FINRLD 187
            INRLD
Sbjct: 181 MINRLD 182

The following BLAST results are available for this feature:

BLAST of Cla011134 vs. Swiss-Prot


Match Name	E-value	Identity	Description
21KD_DAUCA	3.2e-24	35.91	21 kDa protein OS=Daucus carota PE=2 SV=1	[more]
PME3_ARATH	4.6e-15	30.30	Pectinesterase/pectinesterase inhibitor 3 OS=Arabidopsis thaliana GN=PME3 PE=2 S...	[more]
PMEU1_SOLLC	1.5e-13	25.26	Pectinesterase/pectinesterase inhibitor U1 OS=Solanum lycopersicum GN=PMEU1 PE=2...	[more]
PME2_ARATH	5.3e-11	26.79	Pectinesterase 2 OS=Arabidopsis thaliana GN=PME2 PE=2 SV=2	[more]
PME32_ARATH	5.3e-11	32.57	Probable pectinesterase/pectinesterase inhibitor 32 OS=Arabidopsis thaliana GN=P...	[more]

BLAST of Cla011134 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LWR8_CUCSA	9.3e-79	81.12	Uncharacterized protein OS=Cucumis sativus GN=Csa_1G597770 PE=4 SV=1	[more]
A0A061GSW0_THECC	2.0e-49	58.60	Plant invertase/pectin methylesterase inhibitor superfamily protein OS=Theobroma...	[more]
B9RR57_RICCO	3.8e-48	59.22	21 kDa protein, putative OS=Ricinus communis GN=RCOM_0710110 PE=4 SV=1	[more]
U5GAM6_POPTR	9.3e-47	55.10	Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0006s14010g PE=4 SV=1	[more]
F6HGM6_VITVI	2.7e-46	61.11	Putative uncharacterized protein OS=Vitis vinifera GN=VIT_11s0016g00590 PE=4 SV=...	[more]

BLAST of Cla011134 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449452771\|ref\|XP_004144132.1\|	1.3e-78	81.12	PREDICTED: 21 kDa protein [Cucumis sativus]	[more]
gi\|700211234\|gb\|KGN66330.1\|	1.3e-78	81.12	hypothetical protein Csa_1G597770 [Cucumis sativus]	[more]
gi\|659100173\|ref\|XP_008450965.1\|	3.3e-77	79.38	PREDICTED: 21 kDa protein [Cucumis melo]	[more]
gi\|720018304\|ref\|XP_010261745.1\|	3.4e-50	57.59	PREDICTED: 21 kDa protein-like [Nelumbo nucifera]	[more]
gi\|590574990\|ref\|XP_007012560.1\|	2.9e-49	58.60	Plant invertase/pectin methylesterase inhibitor superfamily protein [Theobroma c...	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **INTERPRO**
Term	Definition
IPR006501	Pectinesterase_inhib_dom

Vocabulary: **Molecular Function**
Term	Definition
GO:0004857	enzyme inhibitor activity

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
biological_process	GO:0005982	starch metabolic process
biological_process	GO:0005985	sucrose metabolic process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004857	enzyme inhibitor activity
molecular_function	GO:0030599	pectinesterase activity

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cla011134	Cla011134	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cla011134	Cla011134.1-protein	polypeptide

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cla011134.1.cds1	Cla011134.1.cds1	CDS

InterPro

Analysis Name: InterPro Annotations of watermelon (97103)
Date Performed: 2016-09-28


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	GENE3D	G3DSA:1.20.140.40		coord: 26..185 score: 2.4
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 29..179 score: 1.9
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 26..181 score: 3.7
IPR006501	Pectinesterase inhibitor domain	TIGRFAMs	TIGR01614	TIGR01614	coord: 6..185 score: 1.2
IPR006501	Pectinesterase inhibitor domain	unknown	SSF101148	Plant invertase/pectin methylesterase inhibitor	coord: 28..185 score: 2.49
None	No IPR available	PANTHER	PTHR31080	FAMILY NOT NAMED	coord: 1..194 score: 2.2
None	No IPR available	PANTHER	PTHR31080:SF26	SUBFAMILY NOT NAMED	coord: 1..194 score: 2.2