Cla010518.1 (mRNA) Watermelon (97103) v1

NameCla010518
TypemRNA
OrganismCitrullus. lanatus (Watermelon (97103) v1)
DescriptionCytochrome P450 (AHRD V1 ***- B9NAD7_POPTR); contains Interpro domain(s) IPR001128 Cytochrome P450
LocationChr2 : 12246290 .. 12248749 (+)
Sequence length1305
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTCTCCGATTCGGTTCCCGTCTTGTCGTAGTTGTTTCGTCTCCCTCTGCCGTGAAAGAATGCTTTACAAAGAACGATATCATTCTTGCAAATCGGCCTTTGTTGAACACTACAAAGTGCTTAGCATACAACCAAACCACTATGGGGGTCTCCCCTTACGGTGAACATTGGCGAAACCTCCGTCGAATCGGCTCGTATGAGATTTTCTCAAAAAATCGCCTCAATTTCTTCTTGGGAATTCGGGAAGATGAAGTTAAACGTTTGCTATGTAAATTATGTGGTAATTGTTATAAGTTGGAAGATGAGTTTAGAGTAGTGGAATTGGAGCCTATGTTGCAAGATCTTACATCGAATATTGTTATGAGAATGGTGTGTGGGAAGAAGTTTTATGAAGAAGATTATTGTAGCAAGTTTAGAGATATTGTGACACAAATTCTGGCACATGGTGGGGCCACAAATCCAGGAGACTTCATACCCTTATGGAATTGGATTGATCCAACTGGTTTTATGAAGAGGGTAAAGAAGATTGCAAAGACATCAGATGAATTTCTTCAACAGTTAATTGATGAGATTAGGAACCAAAATGATGGAGGGGACACTATGATTCACCATTTACTTAGTTTGCAGAATATTGAACCTCAATATTACAACGATCAAACTATCAAAGGACTCATTCAAGTATACACATTCCTCTCTCTCCACCTTTTTTTTATGGTTAAATTACAAGCTTAGTCTTTGAACATGTTTTAAGATTTTGTCTATTTATATATCTTTAAACTTTTGAAAGCATTTAGTATGTTCTAAAATTTTCAATTTTGTGTCAAATAGGTTACTTAAATATTTTAAGAAAGTCTCTAATAGATTCCTAAATGTTTAATTTCATATGTCCACTAGGTTCATAAATGGTAAAAGGTGTCAAGTAGATCCTTAAACTTTTATTTTTTTTCCATTAGATCGTTAATTTTCTTTTATATATATCTAATAGATTATTGACCTATTCGACATATATGACATATATTTTTAACTTCACATATCTCTACGAAACATGAAATTAAAAATTTTAGAGTTTCATTAGACATATAATTCAATCTTAATATAAAATGAAATTATCAATTAAGATTTTAAGAGAAAATGAGTATACGAAAGACATATTAGATACAAACCTGAAAGGTTAGGAACATCTTAATATAGTAGACAAAAAAATAAAATTAAATAAAGTTTAGAGACTTATTGGATATAAAACTGAAAGTTCATGATACGCTTTCTAAAGTTTAAGAACCAAATAGACATTAAAATGCAAAAACTAAACTTCTAACTTTTTTTTTTTTTTTTTAATATGATAATTTCTTCTCAATTCGTTGTCTCTCAACATGTTGAAAGTGTTGATGAAATTATGGGATCTTTTGTACATTAGTTAAAAGAATAGGAACTTTTGGACATAATCGGAATATGTGGGCTTATTAGTGTCTTATTGTGTGCATAAAACCCAATGTAAAAACACAGATTCTGGATAATAAGTATTTAGGCCTAGTTTCAATCTCTAAAAGCATTGAACATTAGAGTATTCTACAGAAATCTGTCTTAGAAAATACTTAGAACATATTGACAAAATTAATTCTAACAGAAAATACTTAGAATATATTACCAAAATTCTTCTAACCATAAATTCAAGGGTGCCCACTAATTTAAATAGGAGTGATATCTTAAGAAGTCGTATCAAATCAAGAAAGAAAAACAAAAAGTGATCAATAAACACAATAAGTAGTATATTTCTCAAATGAAATTACTTGAAACATCGCAAAGCTTGTTACCAGAAGTGTTTGGTTTTTGTAGGATATATTTTTAGCTGGGATAGACACGTTAGCTTTGACTTTGGGGTGGGCACTATCTCACTTGCTCAACAATCCAGAGGTGTTAAAGAAGGCAAAAGTTGAGATAGATTGTTGGATTGGGGAAGAACGTGTAGTTAATGAAGCTGATGTACCAAGTTTGAGTTACCTTCAAGGAATAATCACCGAGACTTTGCGTCTTAACCCACCTGCTCCTCTGCTTGTGCCACACTGTGCATTATTTGAAGAGTGCAAAATAGAAGGATATGATGTTCCACGTGATACAATTATATTGATTAATGCTTGGGCTATCCATAGAGATCCAAGTCTTTGGGAAGATGCAACCAGCTTTAAACCTGAAAGACATGCAAGTGGGATTGAAGCTTCAGAGTCATATAAACTAATACCATTTGGAGCGGGGAGGAGAGCATGTCCTGGTGTGGGAATGGCGCAACGTATACTTGCCTTCACTTTAGCGTCCTTGATACAAAGCTTTGAATGGGAAAGAGCATTAATTGATATGAGTGAAGGTCATGGGCTCACAATGCCCAAAGCTCAACCATTGGTAGCTAAGTGTAAACCATGCCCAATCCTGAAAGCCATCCTGAAAGCACGTACCAATTGCTAA

mRNA sequence

ATGTCTCTCCGATTCGGTTCCCGTCTTGTCGTAGTTGTTTCGTCTCCCTCTGCCGTGAAAGAATGCTTTACAAAGAACGATATCATTCTTGCAAATCGGCCTTTGTTGAACACTACAAAGTGCTTAGCATACAACCAAACCACTATGGGGGTCTCCCCTTACGGTGAACATTGGCGAAACCTCCGTCGAATCGGCTCGTATGAGATTTTCTCAAAAAATCGCCTCAATTTCTTCTTGGGAATTCGGGAAGATGAAGTTAAACGTTTGCTATGTAAATTATGTGGTAATTGTTATAAGTTGGAAGATGAGTTTAGAGTAGTGGAATTGGAGCCTATGTTGCAAGATCTTACATCGAATATTGTTATGAGAATGGTGTGTGGGAAGAAGTTTTATGAAGAAGATTATTGTAGCAAGTTTAGAGATATTGTGACACAAATTCTGGCACATGGTGGGGCCACAAATCCAGGAGACTTCATACCCTTATGGAATTGGATTGATCCAACTGGTTTTATGAAGAGGGTAAAGAAGATTGCAAAGACATCAGATGAATTTCTTCAACAGTTAATTGATGAGATTAGGAACCAAAATGATGGAGGGGACACTATGATTCACCATTTACTTAGTTTGCAGAATATTGAACCTCAATATTACAACGATCAAACTATCAAAGGACTCATTCAAGATATATTTTTAGCTGGGATAGACACGTTAGCTTTGACTTTGGGGTGGGCACTATCTCACTTGCTCAACAATCCAGAGGTGTTAAAGAAGGCAAAAGTTGAGATAGATTGTTGGATTGGGGAAGAACGTGTAGTTAATGAAGCTGATGTACCAAGTTTGAGTTACCTTCAAGGAATAATCACCGAGACTTTGCGTCTTAACCCACCTGCTCCTCTGCTTGTGCCACACTGTGCATTATTTGAAGAGTGCAAAATAGAAGGATATGATGTTCCACGTGATACAATTATATTGATTAATGCTTGGGCTATCCATAGAGATCCAAGTCTTTGGGAAGATGCAACCAGCTTTAAACCTGAAAGACATGCAAGTGGGATTGAAGCTTCAGAGTCATATAAACTAATACCATTTGGAGCGGGGAGGAGAGCATGTCCTGGTGTGGGAATGGCGCAACGTATACTTGCCTTCACTTTAGCGTCCTTGATACAAAGCTTTGAATGGGAAAGAGCATTAATTGATATGAGTGAAGGTCATGGGCTCACAATGCCCAAAGCTCAACCATTGGTAGCTAAGTGTAAACCATGCCCAATCCTGAAAGCCATCCTGAAAGCACGTACCAATTGCTAA

Coding sequence (CDS)

ATGTCTCTCCGATTCGGTTCCCGTCTTGTCGTAGTTGTTTCGTCTCCCTCTGCCGTGAAAGAATGCTTTACAAAGAACGATATCATTCTTGCAAATCGGCCTTTGTTGAACACTACAAAGTGCTTAGCATACAACCAAACCACTATGGGGGTCTCCCCTTACGGTGAACATTGGCGAAACCTCCGTCGAATCGGCTCGTATGAGATTTTCTCAAAAAATCGCCTCAATTTCTTCTTGGGAATTCGGGAAGATGAAGTTAAACGTTTGCTATGTAAATTATGTGGTAATTGTTATAAGTTGGAAGATGAGTTTAGAGTAGTGGAATTGGAGCCTATGTTGCAAGATCTTACATCGAATATTGTTATGAGAATGGTGTGTGGGAAGAAGTTTTATGAAGAAGATTATTGTAGCAAGTTTAGAGATATTGTGACACAAATTCTGGCACATGGTGGGGCCACAAATCCAGGAGACTTCATACCCTTATGGAATTGGATTGATCCAACTGGTTTTATGAAGAGGGTAAAGAAGATTGCAAAGACATCAGATGAATTTCTTCAACAGTTAATTGATGAGATTAGGAACCAAAATGATGGAGGGGACACTATGATTCACCATTTACTTAGTTTGCAGAATATTGAACCTCAATATTACAACGATCAAACTATCAAAGGACTCATTCAAGATATATTTTTAGCTGGGATAGACACGTTAGCTTTGACTTTGGGGTGGGCACTATCTCACTTGCTCAACAATCCAGAGGTGTTAAAGAAGGCAAAAGTTGAGATAGATTGTTGGATTGGGGAAGAACGTGTAGTTAATGAAGCTGATGTACCAAGTTTGAGTTACCTTCAAGGAATAATCACCGAGACTTTGCGTCTTAACCCACCTGCTCCTCTGCTTGTGCCACACTGTGCATTATTTGAAGAGTGCAAAATAGAAGGATATGATGTTCCACGTGATACAATTATATTGATTAATGCTTGGGCTATCCATAGAGATCCAAGTCTTTGGGAAGATGCAACCAGCTTTAAACCTGAAAGACATGCAAGTGGGATTGAAGCTTCAGAGTCATATAAACTAATACCATTTGGAGCGGGGAGGAGAGCATGTCCTGGTGTGGGAATGGCGCAACGTATACTTGCCTTCACTTTAGCGTCCTTGATACAAAGCTTTGAATGGGAAAGAGCATTAATTGATATGAGTGAAGGTCATGGGCTCACAATGCCCAAAGCTCAACCATTGGTAGCTAAGTGTAAACCATGCCCAATCCTGAAAGCCATCCTGAAAGCACGTACCAATTGCTAA

Protein sequence

MSLRFGSRLVVVVSSPSAVKECFTKNDIILANRPLLNTTKCLAYNQTTMGVSPYGEHWRNLRRIGSYEIFSKNRLNFFLGIREDEVKRLLCKLCGNCYKLEDEFRVVELEPMLQDLTSNIVMRMVCGKKFYEEDYCSKFRDIVTQILAHGGATNPGDFIPLWNWIDPTGFMKRVKKIAKTSDEFLQQLIDEIRNQNDGGDTMIHHLLSLQNIEPQYYNDQTIKGLIQDIFLAGIDTLALTLGWALSHLLNNPEVLKKAKVEIDCWIGEERVVNEADVPSLSYLQGIITETLRLNPPAPLLVPHCALFEECKIEGYDVPRDTIILINAWAIHRDPSLWEDATSFKPERHASGIEASESYKLIPFGAGRRACPGVGMAQRILAFTLASLIQSFEWERALIDMSEGHGLTMPKAQPLVAKCKPCPILKAILKARTNC
BLAST of Cla010518 vs. Swiss-Prot
Match: C81E9_MEDTR (Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula GN=CYP81E9 PE=1 SV=1)

HSP 1 Score: 437.2 bits (1123), Expect = 2.1e-121
Identity = 234/441 (53.06%), Postives = 295/441 (66.89%), Query Frame = 1

Query: 2   SLRFGSRLVVVVSSPSAVKECFTKNDIILANRPLLNTTKCLAYNQTTMGVSPYGEHWRNL 61
           SL FGSRLVVVVSSPS   ECFTKNDIILANRP   T K + YN TT+G + YG+HWRNL
Sbjct: 66  SLWFGSRLVVVVSSPSLAHECFTKNDIILANRPRFLTGKYIFYNYTTLGSASYGDHWRNL 125

Query: 62  RRIGSYEIFSKNRLNFFLGIREDEVKRLLCKLCGNCYKLEDEFRVVELEPMLQDLTSNIV 121
           RRI + ++ S NRLN FLG+R DE  RL+ KL  +       F  VEL P L ++T N +
Sbjct: 126 RRITTIDVLSNNRLNSFLGVRRDETNRLIQKLLKDVVSEGFGFTKVELRPRLTEMTFNAM 185

Query: 122 MRMVCGKKFYEED-------YCSKFRDIVTQILAHGGATNPGDFIPLWNWIDPTGFMKRV 181
           MRM+ GK++Y +D          +FR+I++++++  GA N GDF+PL   +D     KR 
Sbjct: 186 MRMISGKRYYGDDGDVSDVEEAKQFREIISEMMSLLGANNKGDFLPLLRVVDLDNLEKRC 245

Query: 182 KKIAKTSDEFLQQLIDEIRNQN---DGGDTMIHHLLSLQNIEPQYYNDQTIKGLIQDIFL 241
           K+IAK S+ FL+ LI+E R  N   DGG TMI HLL L   +P+YY+D  IKGLIQ + L
Sbjct: 246 KRIAKRSNAFLEGLIEEHRRGNIHSDGG-TMIDHLLKLSESQPEYYSDHLIKGLIQGMLL 305

Query: 242 AGIDTLALTLGWALSHLLNNPEVLKKAKVEIDCWIGEERVVNEADVPSLSYLQGIITETL 301
           AG DT A+T+ W +S LLN+PEVLKKAK E+D  IG+ ++V+E D+  L YLQ II+ETL
Sbjct: 306 AGTDTSAVTIEWVMSELLNHPEVLKKAKEELDTQIGKNKLVDEQDLSKLPYLQNIISETL 365

Query: 302 RLNPPAPLLVPHCALFEECKIEGYDVPRDTIILINAWAIHRDPSLWEDATSFKPERHASG 361
           RL+PPAPLL+PH +  E+C I  ++VP+DTIIL N W IHRDP  W DA SFKPER    
Sbjct: 366 RLHPPAPLLLPHYS-SEDCTIGEFNVPKDTIILTNVWGIHRDPKHWNDALSFKPERFE-- 425

Query: 362 IEASESYKLIPFGAGRRACPGVGMAQRILAFTLASLIQSFEWER---ALIDMSEGHGLTM 421
            +  E  K++ FG GRRACPG+ +AQR + FT+  LIQ FEWER     +DM EG G+TM
Sbjct: 426 -KEEEVNKVMAFGLGRRACPGLSLAQRTVGFTVGLLIQCFEWERESEEKLDMMEGKGITM 485

Query: 422 PKAQPLVAKCKPCPILKAILK 430
           P   PL A CK  PI   + K
Sbjct: 486 PMKIPLRAMCKALPIANDVTK 501

BLAST of Cla010518 vs. Swiss-Prot
Match: C81E1_GLYEC (Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata GN=CYP81E1 PE=1 SV=2)

HSP 1 Score: 425.6 bits (1093), Expect = 6.4e-118
Identity = 221/438 (50.46%), Postives = 299/438 (68.26%), Query Frame = 1

Query: 2   SLRFGSRLVVVVSSPSAVKECFTKNDIILANRPLLNTTKCLAYNQTTMGVSPYGEHWRNL 61
           SL FGSRLVVVVSS S  ++CFTKND++LANRP   + K + YN TT+G + YGEHWRNL
Sbjct: 68  SLWFGSRLVVVVSSASEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWRNL 127

Query: 62  RRIGSYEIFSKNRLNFFLGIREDEVKRLLCKLCGNCYKLEDEFRVVELEPMLQDLTSNIV 121
           RRI + ++ S +R+N F GIR DE +RL+ +L  +       F  +EL   L D+T N +
Sbjct: 128 RRITALDVLSNHRINSFSGIRRDETQRLITRLADDS---STNFAEMELSSRLYDMTFNNI 187

Query: 122 MRMVCGKKFYEEDY-------CSKFRDIVTQILAHGGATNPGDFIPLWNWIDPTGFMKRV 181
           MRM+ GK++Y ED         S+FRD+V+++L   GA N  DF+PL  ++D     KR+
Sbjct: 188 MRMISGKRYYGEDCDTSDLQEASQFRDMVSELLQLSGANNKTDFMPLLRFLDFENLEKRL 247

Query: 182 KKIAKTSDEFLQQLIDEIRNQNDGGDTMIHHLLSLQNIEPQYYNDQTIKGLIQDIFLAGI 241
           K I+  +D FL+ LI+E R + +  +TMI HLL+LQ+ +P+YY DQ IKGL   + LAG 
Sbjct: 248 KDISGKTDAFLRGLIEEHRTKKERANTMIDHLLNLQDSQPEYYTDQIIKGLALAMLLAGT 307

Query: 242 DTLALTLGWALSHLLNNPEVLKKAKVEIDCWIGEERVVNEADVPSLSYLQGIITETLRLN 301
           D+ A+TL W++S+LLN+PEVLKK K E+D  +G++R+V+E+D+P L+YL+ +I ETLRL 
Sbjct: 308 DSSAVTLEWSMSNLLNHPEVLKKVKDELDTHVGQDRLVDESDLPKLTYLKNVINETLRLY 367

Query: 302 PPAPLLVPHCALFEECKIEGYDVPRDTIILINAWAIHRDPSLWEDATSFKPERHASGIEA 361
            PAPLL+PH +  +EC I GY VP+DTI+LINAWAIHRDP LW +AT+FKPER     + 
Sbjct: 368 TPAPLLLPH-STSDECNIGGYKVPQDTIVLINAWAIHRDPELWTEATTFKPERFE---KK 427

Query: 362 SESYKLIPFGAGRRACPGVGMAQRILAFTLASLIQSFEWERA---LIDMSEGHGLTMPKA 421
            E  KLI FG GRRACPG G+A R ++ TLA LIQ F+W+      ID++E  G T+ K 
Sbjct: 428 GELEKLIAFGMGRRACPGEGLAIRAISMTLALLIQCFDWKLINGDKIDLAERDGFTLTKL 487

Query: 422 QPLVAKCKPCPILKAILK 430
            PL A CK  P++  + K
Sbjct: 488 VPLKAMCKSRPVINKVFK 498

BLAST of Cla010518 vs. Swiss-Prot
Match: C8D11_ARATH (Cytochrome P450 81D11 OS=Arabidopsis thaliana GN=CYP81D11 PE=2 SV=1)

HSP 1 Score: 417.9 bits (1073), Expect = 1.3e-115
Identity = 228/438 (52.05%), Postives = 291/438 (66.44%), Query Frame = 1

Query: 2   SLRFGSRLVVVVSSPSAVKECFTKNDIILANRPLLNTTKCLAYNQTTMGVSPYGEHWRNL 61
           SL  GSRLV VVSS +  +ECFTKND++LANRP     K + YN TTM  + YG+ WRNL
Sbjct: 78  SLSLGSRLVFVVSSHAVAEECFTKNDVVLANRPEFLVGKHIGYNSTTMVGAAYGDSWRNL 137

Query: 62  RRIGSYEIFSKNRLNFFLGIREDEVKRLLCKLCGNCYKLEDEFRVVELEPMLQDLTSNIV 121
           RRIG+ EIFS  RLN F+ IR+DE++RL+  L  N    +  F  VE++P+   LT N +
Sbjct: 138 RRIGTIEIFSSLRLNSFVSIRQDEIRRLIICLAKNS---QHGFVKVEMKPLFMCLTINNI 197

Query: 122 MRMVCGKKFY----EEDYCSKF-RDIVTQILAHGGATNPGDFIPLWNWIDPTGFMKRVKK 181
           +RMV GK+FY    E D  +K  R ++ +++  GGA N  D+ P+  ++  T + K VKK
Sbjct: 198 IRMVAGKRFYGDGTENDNEAKHVRQLIAEVVVSGGAGNAADYFPILRYV--TNYEKHVKK 257

Query: 182 IAKTSDEFLQQLIDEIRNQNDGGDTMIHHLLSLQNIEPQYYNDQTIKGLIQDIFLAGIDT 241
           +A   DEFLQ L++E R +   G+TMI HLLSLQ  +P YY D  IKG+I  + LAG DT
Sbjct: 258 LAGRVDEFLQSLVNEKRVEKVKGNTMIDHLLSLQETQPDYYTDVIIKGIILVMILAGTDT 317

Query: 242 LALTLGWALSHLLNNPEVLKKAKVEIDCWIGEERVVNEADVPSLSYLQGIITETLRLNPP 301
            A TL WA+S+LLN+PEVL+KAK EID  IG +R+V E D+  L YLQ I++ETLRL P 
Sbjct: 318 SAGTLEWAMSNLLNHPEVLRKAKTEIDDQIGVDRLVEEQDIVKLPYLQHIVSETLRLYPV 377

Query: 302 APLLVPHCALFEECKIEGYDVPRDTIILINAWAIHRDPSLWEDATSFKPERHASGIEASE 361
           AP+L+PH A  E+C ++GYDVPR TIIL+NAWAIHRDP LWE+   FKPER     +  E
Sbjct: 378 APMLLPHLA-SEDCIVDGYDVPRGTIILVNAWAIHRDPKLWEEPEKFKPERFE---KKGE 437

Query: 362 SYKLIPFGAGRRACPGVGMAQRILAFTLASLIQSFEWERA---LIDMSEGH-GLTMPKAQ 421
             KL+PFG GRR+CPG G+AQR++   L SL+Q FEWER     +DM E   G TM KA 
Sbjct: 438 DKKLMPFGIGRRSCPGSGLAQRLVTLALGSLVQCFEWERVEEKYLDMRESEKGTTMRKAT 497

Query: 422 PLVAKCKPCPILKAILKA 431
            L A CK  PI+  +L A
Sbjct: 498 SLQAMCKARPIVHKVLDA 506

BLAST of Cla010518 vs. Swiss-Prot
Match: C81E7_MEDTR (Isoflavone 2'-hydroxylase OS=Medicago truncatula GN=CYP81E7 PE=1 SV=1)

HSP 1 Score: 416.4 bits (1069), Expect = 3.9e-115
Identity = 219/439 (49.89%), Postives = 295/439 (67.20%), Query Frame = 1

Query: 1   MSLRFGSRLVVVVSSPSAVKECFTKNDIILANRPLLNTTKCLAYNQTTMGVSPYGEHWRN 60
           +SL FGSRLVVVVSS S  +ECFTKND++LANRP   + K + YN TT+G + YGEHWRN
Sbjct: 67  ISLWFGSRLVVVVSSLSEFQECFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWRN 126

Query: 61  LRRIGSYEIFSKNRLNFFLGIREDEVKRLLCKLCGNCYKLEDEFRVVELEPMLQDLTSNI 120
           LRRI S ++ S +R+N F  IR DE +RL+ KL  +      +F  VEL     D+T N 
Sbjct: 127 LRRITSLDVLSNHRINNFAPIRRDETQRLIKKLAEDS---STKFAEVELTFRFFDMTFNN 186

Query: 121 VMRMVCGKKFYEEDY-------CSKFRDIVTQILAHGGATNPGDFIPLWNWIDPTGFMKR 180
           +MRM+ GK++Y +D         S+FRD+V+++L   GA N  DF+PL  ++D     KR
Sbjct: 187 IMRMISGKRYYGDDCDISEVQEASQFRDMVSELLQLSGANNKTDFMPLLKFLDFENLEKR 246

Query: 181 VKKIAKTSDEFLQQLIDEIRNQNDGGDTMIHHLLSLQNIEPQYYNDQTIKGLIQDIFLAG 240
           VK+I + +D FL  L+ E R++ +  +TMI HLL++Q  +P+YY D  IKGL   + LAG
Sbjct: 247 VKRIGEKNDVFLSGLLQEQRSKKERTNTMIDHLLNMQESQPEYYTDTIIKGLCLAMLLAG 306

Query: 241 IDTLALTLGWALSHLLNNPEVLKKAKVEIDCWIGEERVVNEADVPSLSYLQGIITETLRL 300
            D+ A+TL W +S++LN PEVLKK + E+D  +G++R+V+E+D+P L+YL+ +I ETLRL
Sbjct: 307 TDSSAVTLEWTMSNILNYPEVLKKVRDEVDTHVGQDRLVDESDLPKLTYLRNVIYETLRL 366

Query: 301 NPPAPLLVPHCALFEECKIEGYDVPRDTIILINAWAIHRDPSLWEDATSFKPERHASGIE 360
             PAPLL+PH +  +EC + GY VPRDTI+LINAWAIHRDP  W +AT+FKPER     +
Sbjct: 367 YTPAPLLLPH-STADECIMGGYKVPRDTIVLINAWAIHRDPETWSEATTFKPERFD---K 426

Query: 361 ASESYKLIPFGAGRRACPGVGMAQRILAFTLASLIQSFEWER---ALIDMSEGHGLTMPK 420
             E  K+I FG GRRACPG G+A R ++ TLA L+Q F+W+R     IDMSE  G TM K
Sbjct: 427 KGELEKMIAFGMGRRACPGEGLALRAISMTLALLVQCFDWKRINDEKIDMSERDGFTMTK 486

Query: 421 AQPLVAKCKPCPILKAILK 430
             PL A CK  P++  + K
Sbjct: 487 LLPLKAMCKTRPVVNKVFK 498

BLAST of Cla010518 vs. Swiss-Prot
Match: C81E8_MEDTR (Cytochrome P450 81E8 OS=Medicago truncatula GN=CYP81E8 PE=2 SV=1)

HSP 1 Score: 414.1 bits (1063), Expect = 1.9e-114
Identity = 222/428 (51.87%), Postives = 281/428 (65.65%), Query Frame = 1

Query: 2   SLRFGSRLVVVVSSPSAVKECFTKNDIILANRPLLNTTKCLAYNQTTMGVSPYGEHWRNL 61
           SL FGSRLVVVVSS +  +ECFTKNDI+LANRP   T K + YN TT+  SPYG+HWRNL
Sbjct: 68  SLWFGSRLVVVVSSLTIAQECFTKNDIVLANRPHFLTGKYIGYNNTTVAQSPYGDHWRNL 127

Query: 62  RRIGSYEIFSKNRLNFFLGIREDEVKRLLCKLCGNCYKLEDEFRVVELEPMLQDLTSNIV 121
           RRI S EI S +RLN FL IR DE+ RL+ KL    Y   + F  VEL PM  ++T N +
Sbjct: 128 RRILSIEILSSHRLNSFLEIRRDEIMRLIQKLAQKSY---NGFTEVELRPMFSEMTFNTI 187

Query: 122 MRMVCGKKFYEEDY-------CSKFRDIVTQILAHGGATNPGDFIPLWNWIDPTGFMKRV 181
           MRMV GK++Y  D           FR I+ ++++ GGA N GDF+    W D  G  KR+
Sbjct: 188 MRMVSGKRYYGNDCDVSDVEEARLFRGIIKEVVSLGGANNVGDFLGFLRWFDFDGLEKRL 247

Query: 182 KKIAKTSDEFLQQLIDEIRNQNDGGDTMIHHLLSLQNIEPQYYNDQTIKGLIQDIFLAGI 241
           KKI+K +D FLQ LIDE R      +TMI HLL+ Q  +P+YY DQ IKGL+  + LAG 
Sbjct: 248 KKISKRTDAFLQGLIDEHRFGKRNSNTMIDHLLTQQQSQPEYYTDQIIKGLMVVMLLAGT 307

Query: 242 DTLALTLGWALSHLLNNPEVLKKAKVEIDCWIGEERVVNEADVPSLSYLQGIITETLRLN 301
           DT ++T+ WA+S+LLN+PE++KKAK E+D  IG +R V+E D+  L YLQ I+ ETLRL+
Sbjct: 308 DTSSVTIEWAMSNLLNHPEIMKKAKNELDTHIGHDRQVDEHDISKLPYLQSIVYETLRLH 367

Query: 302 PPAPLLVPHCALFEECKIEGYDVPRDTIILINAWAIHRDPSLWEDATSFKPERHASGIEA 361
             APLLVPH +  E+  + GY++P++TI+++NAW IHRDP+LW D T FKPER     + 
Sbjct: 368 AAAPLLVPHLS-SEDFSLGGYNIPQNTILMVNAWVIHRDPNLWSDPTCFKPERFE---KE 427

Query: 362 SESYKLIPFGAGRRACPGVGMAQRILAFTLASLIQSFEWER---ALIDMSEGHGLTMPKA 420
            E  KL+ FG GRRACPG  ++QR    TL  LIQ FEW+R     IDM E  G+T  K 
Sbjct: 428 GEVNKLLSFGLGRRACPGENLSQRTEGLTLGLLIQCFEWKRIGEEKIDMVEAKGITAGKK 487

BLAST of Cla010518 vs. TrEMBL
Match: A0A0A0LQI5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G423640 PE=3 SV=1)

HSP 1 Score: 617.1 bits (1590), Expect = 1.7e-173
Identity = 306/441 (69.39%), Postives = 359/441 (81.41%), Query Frame = 1

Query: 2   SLRFGSRLVVVVSSPSAVKECFTKNDIILANRPLLNTTKCLAYNQTTMGVSPYGEHWRNL 61
           SL+FGSRLV++VSSPSAV+ECFTKNDIILANRP LN+ K L+YN TTM VSPYGEHWRNL
Sbjct: 68  SLKFGSRLVLLVSSPSAVQECFTKNDIILANRPSLNSGKYLSYNNTTMAVSPYGEHWRNL 127

Query: 62  RRIGSYEIFSKNRLNFFLGIREDEVKRLLCKLCGNCYKLEDEFRVVELEPMLQDLTSNIV 121
           RRI + EIFS  RLN F  IRE+EVKRLLCKLCGN YK EDEFRVVELE ML DLTSNIV
Sbjct: 128 RRICTLEIFSTTRLNSFSRIREEEVKRLLCKLCGN-YKFEDEFRVVELESMLLDLTSNIV 187

Query: 122 MRMVCGKKFYEED-----------YCSKFRDIVTQILAHGGATNPGDFIPLWNWIDPTGF 181
           MRMV GKKF +E+           YC + +++VTQI+AH G+TNPGDFIPLWNWIDP+G+
Sbjct: 188 MRMVGGKKFCKENNKNINVLEDEGYCKRLKELVTQIMAHAGSTNPGDFIPLWNWIDPSGY 247

Query: 182 MKRVKKIAKTSDEFLQQLIDEIRNQNDGGDTMIHHLLSLQNIEPQYYNDQTIKGLIQDIF 241
            KR+ KI +  DE LQ+L+DEIRN+ D G+TMI HLL LQ  +P+YY+D  IKGLIQDI 
Sbjct: 248 NKRIMKIGRRMDEVLQRLVDEIRNEEDEGNTMIQHLLRLQKTDPKYYSDLIIKGLIQDIL 307

Query: 242 LAGIDTLALTLGWALSHLLNNPEVLKKAKVEIDCWIGEERVVNEADVPSLSYLQGIITET 301
           +AGIDT A+TL WALSHLLNNP VL KAK EID +IG+ER+VNE D+ SLSYLQGII+ET
Sbjct: 308 IAGIDTSAVTLQWALSHLLNNPIVLDKAKAEIDSYIGQERMVNEVDLSSLSYLQGIISET 367

Query: 302 LRLNPPAPLLVPHCALFEECKIEGYDVPRDTIILINAWAIHRDPSLWEDATSFKPERHAS 361
           LRL+PP PLLVPHCA  E+CKI GYDVPR+TI+LINAWAIHRDP++WEDA SFKPERH +
Sbjct: 368 LRLSPPGPLLVPHCA-SEDCKIGGYDVPRNTIVLINAWAIHRDPNVWEDAGSFKPERHVN 427

Query: 362 GIEASESYKLIPFGAGRRACPGVGMAQRILAFTLASLIQSFEWER---ALIDMSEGHGLT 421
            +    SYKL+PFG GRRACPG+ MAQR++  TLASLIQ FEW++    L+DM EG GLT
Sbjct: 428 AVGFENSYKLLPFGLGRRACPGMAMAQRVVGLTLASLIQCFEWKKMSNLLVDMREGEGLT 487

Query: 422 MPKAQPLVAKCKPCPILKAIL 429
           MPK + LVAKC+P  I+K +L
Sbjct: 488 MPKVESLVAKCRPRFIMKVVL 506

BLAST of Cla010518 vs. TrEMBL
Match: A0A0A0LTD0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G425740 PE=3 SV=1)

HSP 1 Score: 590.5 bits (1521), Expect = 1.7e-165
Identity = 294/437 (67.28%), Postives = 354/437 (81.01%), Query Frame = 1

Query: 2   SLRFGSRLVVVVSSPSAVKECFTKNDIILANRPLLNTTKCLAYNQTTMGVSPYGEHWRNL 61
           SLRFGS LVVV+SSPSAV+ECFTKNDIILANRP LNT K L+YN T + V+PYGE WR L
Sbjct: 67  SLRFGSHLVVVISSPSAVRECFTKNDIILANRPFLNTGKHLSYNFTVLAVAPYGELWRRL 126

Query: 62  RRIGSYEIFSKNRLNFFLGIREDEVKRLLCKLCGNCYKLEDE--FRVVELEPMLQDLTSN 121
           RRI + EIFS ++LN F  IR+DEVKRLL KLC N   +E+   F  VELEP+L D TSN
Sbjct: 127 RRISTCEIFSTSKLNSFSCIRQDEVKRLLHKLC-NRNSVEELLLFSAVELEPILLDFTSN 186

Query: 122 IVMRMVCGKKFYEEDY-----CSKFRDIVTQILAHGGATNPGDFIPLWNWIDPTGFMKRV 181
           IVMRMV GK+++ +D        KFRD+V +++ + GATNPGDFIPLWNWIDPTG  K++
Sbjct: 187 IVMRMVGGKRYFGDDVLDEGQAEKFRDVVKRVMLYAGATNPGDFIPLWNWIDPTGLEKKI 246

Query: 182 KKIAKTSDEFLQQLIDEIRNQNDGGDTMIHHLLSLQNIEPQYYNDQTIKGLIQDIFLAGI 241
            K+ + +DE  Q LIDEIRN+ + G+TMIHHLL LQN +P+Y++DQ IKGLI  IFLAGI
Sbjct: 247 MKVGEEADEIFQGLIDEIRNEEEDGNTMIHHLLHLQNTQPEYFSDQIIKGLIHVIFLAGI 306

Query: 242 DTLALTLGWALSHLLNNPEVLKKAKVEIDCWIGEERVVNEADVPSLSYLQGIITETLRLN 301
           DT+++TL W LSHLLNNP+V+KKA++EI+  +G+ER+VNE D+ SLSYLQGII ETLRL 
Sbjct: 307 DTISVTLEWGLSHLLNNPKVIKKARLEIEHIVGQERLVNEDDLSSLSYLQGIILETLRLT 366

Query: 302 PPAPLLVPHCALFEECKIEGYDVPRDTIILINAWAIHRDPSLWEDATSFKPERHASGIEA 361
           P APLLVPHCA  E+C+IEGYD+PR TII +NAWAIHRD SLWED TSFKPERH + IE 
Sbjct: 367 PAAPLLVPHCA-SEDCQIEGYDIPRGTIIFVNAWAIHRDSSLWEDVTSFKPERHENAIEL 426

Query: 362 SESYKLIPFGAGRRACPGVGMAQRILAFTLASLIQSFEWER---ALIDMSEGHGLTMPKA 421
           S+SYKL+PFG GRRACPGVGMAQR+L  TLASLIQ F+WER   +L+DM+EG G+TMPKA
Sbjct: 427 SDSYKLLPFGLGRRACPGVGMAQRVLGLTLASLIQCFDWERMDSSLVDMTEGQGITMPKA 486

Query: 422 QPLVAKCKPCPILKAIL 429
           QPLVAKCKP PI++A L
Sbjct: 487 QPLVAKCKPRPIMRAHL 501

BLAST of Cla010518 vs. TrEMBL
Match: A0A0A0LNA4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G425750 PE=3 SV=1)

HSP 1 Score: 578.6 bits (1490), Expect = 6.5e-162
Identity = 287/431 (66.59%), Postives = 356/431 (82.60%), Query Frame = 1

Query: 2   SLRFGSRLVVVVSSPSAVKECFTKNDIILANRPLLNTTKCLAYNQTTMGVSPYGEHWRNL 61
           SLRFGSRLVV++SSPS V+ECFTKNDIILANRPLL+T K LAYN TTM V+PYGEHWRNL
Sbjct: 67  SLRFGSRLVVIISSPSVVQECFTKNDIILANRPLLDTGKHLAYNHTTMAVAPYGEHWRNL 126

Query: 62  RRIGSYEIFSKNRLNFFLGIREDEVKRLLCKLCGNCYKLEDEFRVVELEPMLQDLTSNIV 121
           RRIG+ E+FS +R+N F  +RE+EVK L+ +LCG+   LE  FR VE+E ML DL  N++
Sbjct: 127 RRIGALELFSTSRINLFSRMREEEVKALMVRLCGSS-SLEG-FRAVEMESMLLDLMYNVI 186

Query: 122 MRMVCGKKFYEEDY--CSKFRDIVTQILAHGGATNPGDFIPLWNWIDPTGFMKRVKKIAK 181
           M M+ GKK  EED     +FR++VT+I+A GGA+NPGDFI +WNWIDP+G  K++ K+ +
Sbjct: 187 MGMMGGKKGCEEDEGKSKEFREMVTKIMAVGGASNPGDFIAIWNWIDPSGLKKKILKLGQ 246

Query: 182 TSDEFLQQLIDEIRNQNDGGDTMIHHLLSLQNIEPQYYNDQTIKGLIQDIFLAGIDTLAL 241
           T D  LQ+L+D +RN++  G+TMIH LL LQ IEP+ ++DQ IKGLIQ I +AGIDT A+
Sbjct: 247 TMDVLLQELVDGMRNESGEGNTMIHRLLQLQKIEPENHSDQIIKGLIQIILIAGIDTAAV 306

Query: 242 TLGWALSHLLNNPEVLKKAKVEIDCWIGEERVVNEADVPSLSYLQGIITETLRLNPPAPL 301
           TL WALSHLLNNP+VL+KAK+EID  +G++R+VNEAD+PSL+YLQGII ETLRL+P APL
Sbjct: 307 TLEWALSHLLNNPDVLEKAKIEIDNVVGQKRLVNEADLPSLTYLQGIIFETLRLSPAAPL 366

Query: 302 LVPHCALFEECKIEGYDVPRDTIILINAWAIHRDPSLWEDATSFKPERHASGIEASESYK 361
           LVPHC+  E+CKI GYDVPRDTI++INAWAIHRDP+LWEDATSFKPERH + I   ESYK
Sbjct: 367 LVPHCS-SEDCKIGGYDVPRDTIVIINAWAIHRDPNLWEDATSFKPERHTNPI-GVESYK 426

Query: 362 LIPFGAGRRACPGVGMAQRILAFTLASLIQSFEWER---ALIDMSEGHGLTMPKAQPLVA 421
            +PFG GRRACPG+G+AQR+   TLA++IQ FEW+R   +L+DMSEG GLTMPKAQPL+A
Sbjct: 427 FLPFGLGRRACPGIGIAQRMTNLTLATMIQCFEWKREGSSLVDMSEGEGLTMPKAQPLIA 486

Query: 422 KCKPCPILKAI 428
           KCKP PI+KA+
Sbjct: 487 KCKPRPIMKAM 493

BLAST of Cla010518 vs. TrEMBL
Match: A0A061EHP2_THECC (Cytochrome P450, family 81, subfamily D, polypeptide 8, putative OS=Theobroma cacao GN=TCM_019147 PE=3 SV=1)

HSP 1 Score: 490.7 bits (1262), Expect = 1.8e-135
Identity = 253/436 (58.03%), Postives = 317/436 (72.71%), Query Frame = 1

Query: 2   SLRFGSRLVVVVSSPSAVKECFTKNDIILANRPLLNTTKCLAYNQTTMGVSPYGEHWRNL 61
           SLRFGSRLVVVVSS +A +ECFTKNDI+LA RP L   K L YN +T+  SPYG+HWRNL
Sbjct: 112 SLRFGSRLVVVVSSSTAAEECFTKNDIVLAYRPKLLIGKYLGYNWSTVVASPYGDHWRNL 171

Query: 62  RRIGSYEIFSKNRLNFFLGIREDEVKRLLCKLCGNCYKLEDEFRVVELEPMLQDLTSNIV 121
           RRIG+ EIFS +RLN FL IR+DEVKRLL KL  +    + EF  VEL+ +  DLT N +
Sbjct: 172 RRIGAIEIFSSSRLNAFLSIRKDEVKRLLMKLSSDDNSGQ-EFTKVELKSLFVDLTFNNI 231

Query: 122 MRMVCGKKFY------EEDYCSKFRDIVTQILAHGGATNPGDFIPLWNWIDPTGFMKRVK 181
           MRMV GK+++      +E    +FR+++ +  A GG  +PGDF+P+ NWI    ++K++ 
Sbjct: 232 MRMVAGKRYHGGDSVTDEGEAKEFRELMKESFASGGVAHPGDFLPILNWIGGRSYLKKLM 291

Query: 182 KIAKTSDEFLQQLIDEIRNQNDGGDTMIHHLLSLQNIEPQYYNDQTIKGLIQDIFLAGID 241
            + +  D  LQ+LIDE+R +  G +TMI HLLSLQ  EP YY DQ IKGLI  + LAG D
Sbjct: 292 NLGERMDRLLQKLIDEVRAKRQG-NTMIDHLLSLQQTEPDYYTDQLIKGLILVMLLAGTD 351

Query: 242 TLALTLGWALSHLLNNPEVLKKAKVEIDCWIGEERVVNEADVPSLSYLQGIITETLRLNP 301
           T A+TL WA+S+LLNNP+VLKKA+ E+D  IG+E +++E DV  L YLQ II ETLRLNP
Sbjct: 352 TSAVTLEWAMSNLLNNPDVLKKARTELDSQIGQENLIDEPDVSKLQYLQSIIFETLRLNP 411

Query: 302 PAPLLVPHCALFEECKIEGYDVPRDTIILINAWAIHRDPSLWEDATSFKPERHASGIEAS 361
             PLL+PH A   +C+I GYDVPRDTI+LINAWAIHRDP+LW+D TSFKPERH +G    
Sbjct: 412 SVPLLLPHMA-STDCRICGYDVPRDTIVLINAWAIHRDPTLWDDPTSFKPERHGNG--ER 471

Query: 362 ESYKLIPFGAGRRACPGVGMAQRILAFTLASLIQSFEWERAL---IDMSEGHGLTMPKAQ 421
           ES KL+PFG GRRACPG G+AQR++  TL SLIQ FEWER     +DM+EG+G+TMPK  
Sbjct: 472 ESNKLMPFGLGRRACPGAGLAQRVVGLTLGSLIQCFEWERVSEKEVDMAEGNGITMPKVV 531

Query: 422 PLVAKCKPCPILKAIL 429
           PL A CK  PI+  +L
Sbjct: 532 PLEAMCKARPIVNKVL 542

BLAST of Cla010518 vs. TrEMBL
Match: A0A0A0KJM0_CUCSA (Cytochrome P450 OS=Cucumis sativus GN=Csa_6G492230 PE=3 SV=1)

HSP 1 Score: 488.4 bits (1256), Expect = 8.9e-135
Identity = 260/441 (58.96%), Postives = 318/441 (72.11%), Query Frame = 1

Query: 1   MSLRFGSRLVVVVSSPSAVKECFTKNDIILANRPLLNTTKCLAYNQTTMGVSPYGEHWRN 60
           +SL FGSRLVVVVSS  AV+ECFTKNDI+LANRP L   K + YN TTM  SPYG+HWRN
Sbjct: 64  ISLWFGSRLVVVVSSLPAVEECFTKNDIVLANRPRLLVGKHVGYNHTTMVASPYGDHWRN 123

Query: 61  LRRIGSYEIFSKNRLNFFLGIREDEVKRLLCKLCGNCYKLEDEFRVVELEPMLQDLTSNI 120
           LRRIG+ EIFS +RLN F G R+DEV+RLL KL  N       F  VE++  + +LT NI
Sbjct: 124 LRRIGAIEIFSASRLNKFAGTRKDEVERLLKKLSRNSMY---GFSKVEMQSSISELTFNI 183

Query: 121 VMRMVCGKKFYEEDY-----CSKFRDIVTQILAHGGATNPGDFIPLWNWIDPTGFMKRVK 180
            MRM  GK+++ E+        + R+++ QI++ GG +NPGDFIP+ NWI P GF ++V 
Sbjct: 184 SMRMAAGKRYFGEEVRDVEEARQSRELIKQIVSMGGVSNPGDFIPMMNWI-PNGFKRKVW 243

Query: 181 KIAKTSDEFLQQLIDEIR-NQNDGGDTMIHHLLSLQNIEPQYYNDQTIKGLIQDIFLAGI 240
           ++AK  D FLQ LIDE R N+ +  +TMI HLLSLQ  EP+YY DQ IKG+I  + LAG 
Sbjct: 244 RVAKRMDGFLQGLIDEHRSNKEEERNTMIDHLLSLQETEPEYYGDQIIKGIILVLLLAGT 303

Query: 241 DTLALTLGWALSHLLNNPEVLKKAKVEIDCWIGEERVVNEADVPSLSYLQGIITETLRLN 300
           DT A+T+ WAL+HLLNNPEVLKKA+ E+D  IGE+R+V E+DV  L YLQGII+ETLRLN
Sbjct: 304 DTSAVTIEWALAHLLNNPEVLKKAREELDTQIGEKRLVEESDVSKLPYLQGIISETLRLN 363

Query: 301 PPAPLLVPHCALFEECKIEGYDVPRDTIILINAWAIHRDPSLWEDATSFKPERHASGIEA 360
           P AP+LVPH     +C I GY +PRDTI+L+NAWAIHRDP  WE+ T FKPERH    + 
Sbjct: 364 PAAPMLVPHLT-SNDCTISGYKIPRDTIVLVNAWAIHRDPHQWEEPTLFKPERH----QK 423

Query: 361 SESY-----KLIPFGAGRRACPGVGMAQRILAFTLASLIQSFEWER---ALIDMSEGHGL 420
           SES      KLIPFG GRRACPG GMAQR++  TLA+LIQ +EWER     +DMSEG G+
Sbjct: 424 SESIDHHISKLIPFGVGRRACPGSGMAQRVVGLTLAALIQCYEWERIGDEKVDMSEGRGV 483

Query: 421 TMPKAQPLVAKCKPCPILKAI 428
           TMPK  PL A CKP PI+  I
Sbjct: 484 TMPKMVPLEAMCKPRPIIHNI 495

BLAST of Cla010518 vs. NCBI nr
Match: gi|659111621|ref|XP_008455824.1| (PREDICTED: cytochrome P450 81D1-like [Cucumis melo])

HSP 1 Score: 656.4 bits (1692), Expect = 3.5e-185
Identity = 322/434 (74.19%), Postives = 372/434 (85.71%), Query Frame = 1

Query: 2   SLRFGSRLVVVVSSPSAVKECFTKNDIILANRPLLNTTKCLAYNQTTMGVSPYGEHWRNL 61
           SLRFGSRLVVVVSSPSAV+ECFTKNDIILANRPLL + K L YN +++G+SPYGE WRNL
Sbjct: 67  SLRFGSRLVVVVSSPSAVQECFTKNDIILANRPLLESGKYLGYNHSSIGLSPYGEQWRNL 126

Query: 62  RRIGSYEIFSKNRLNFFLGIREDEVKRLLCKLCGNCYKLED-EFRVVELEPMLQDLTSNI 121
           RRIGS E+FS  RLN FLGIREDEVKRLL KLCGN  KLED EFRVVE+ P+L+DLT NI
Sbjct: 127 RRIGSLEVFSTKRLNMFLGIREDEVKRLLRKLCGNHCKLEDDEFRVVEIGPLLKDLTLNI 186

Query: 122 VMRMVCGKKFYEEDYCSKFRDIVTQILAHGGATNPGDFIPLWNWIDPTGFMKRVKKIAKT 181
           VMRMV GK FYEED  +KFR+IVTQI+ H GA+NPGDFIPLWNWIDPTGFMK+VKK+ KT
Sbjct: 187 VMRMVSGKMFYEED-SNKFREIVTQIMEHAGASNPGDFIPLWNWIDPTGFMKKVKKLGKT 246

Query: 182 SDEFLQQLIDEIRNQNDGGDTMIHHLLSLQNIEPQYYNDQTIKGLIQDIFLAGIDTLALT 241
           SDEFLQQLID IRNQNDGG+TMIHHLL+LQN+EP++YND   K +IQDI LAGIDT A+T
Sbjct: 247 SDEFLQQLIDGIRNQNDGGNTMIHHLLTLQNVEPEFYNDHAFKAIIQDILLAGIDTSAVT 306

Query: 242 LGWALSHLLNNPEVLKKAKVEIDCWIGEERVVNEADVPSLSYLQGIITETLRLNPPAPLL 301
           L WALSHLLNNP VL+KAK EID +IG+ER+VNEAD+ SLSYLQGII+ETLRL+P  PLL
Sbjct: 307 LQWALSHLLNNPIVLEKAKAEIDSYIGQERMVNEADLSSLSYLQGIISETLRLSPAGPLL 366

Query: 302 VPHCALFEECKIEGYDVPRDTIILINAWAIHRDPSLWEDATSFKPERHASGIEASESYKL 361
           VPHC+  E+CKI GYDVPR+TI+LINAWAIHRDP+LWEDA+SFKPERH +      SYKL
Sbjct: 367 VPHCS-SEDCKIGGYDVPRNTIVLINAWAIHRDPNLWEDASSFKPERHVNAAGFENSYKL 426

Query: 362 IPFGAGRRACPGVGMAQRILAFTLASLIQSFEWER---ALIDMSEGHGLTMPKAQPLVAK 421
           +PFG GRRACPG+ MAQR++  TLASL+Q FEW+R    L+DM EG GLTMPK +PLVAK
Sbjct: 427 LPFGMGRRACPGMAMAQRVIGLTLASLVQCFEWKRMSDLLVDMREGEGLTMPKVEPLVAK 486

Query: 422 CKPCPILKAILKAR 432
           C+PC I++A+L  +
Sbjct: 487 CRPCFIMEAVLSEK 498

BLAST of Cla010518 vs. NCBI nr
Match: gi|778674449|ref|XP_004151924.2| (PREDICTED: cytochrome P450 81D1-like [Cucumis sativus])

HSP 1 Score: 617.1 bits (1590), Expect = 2.4e-173
Identity = 306/441 (69.39%), Postives = 359/441 (81.41%), Query Frame = 1

Query: 2   SLRFGSRLVVVVSSPSAVKECFTKNDIILANRPLLNTTKCLAYNQTTMGVSPYGEHWRNL 61
           SL+FGSRLV++VSSPSAV+ECFTKNDIILANRP LN+ K L+YN TTM VSPYGEHWRNL
Sbjct: 68  SLKFGSRLVLLVSSPSAVQECFTKNDIILANRPSLNSGKYLSYNNTTMAVSPYGEHWRNL 127

Query: 62  RRIGSYEIFSKNRLNFFLGIREDEVKRLLCKLCGNCYKLEDEFRVVELEPMLQDLTSNIV 121
           RRI + EIFS  RLN F  IRE+EVKRLLCKLCGN YK EDEFRVVELE ML DLTSNIV
Sbjct: 128 RRICTLEIFSTTRLNSFSRIREEEVKRLLCKLCGN-YKFEDEFRVVELESMLLDLTSNIV 187

Query: 122 MRMVCGKKFYEED-----------YCSKFRDIVTQILAHGGATNPGDFIPLWNWIDPTGF 181
           MRMV GKKF +E+           YC + +++VTQI+AH G+TNPGDFIPLWNWIDP+G+
Sbjct: 188 MRMVGGKKFCKENNKNINVLEDEGYCKRLKELVTQIMAHAGSTNPGDFIPLWNWIDPSGY 247

Query: 182 MKRVKKIAKTSDEFLQQLIDEIRNQNDGGDTMIHHLLSLQNIEPQYYNDQTIKGLIQDIF 241
            KR+ KI +  DE LQ+L+DEIRN+ D G+TMI HLL LQ  +P+YY+D  IKGLIQDI 
Sbjct: 248 NKRIMKIGRRMDEVLQRLVDEIRNEEDEGNTMIQHLLRLQKTDPKYYSDLIIKGLIQDIL 307

Query: 242 LAGIDTLALTLGWALSHLLNNPEVLKKAKVEIDCWIGEERVVNEADVPSLSYLQGIITET 301
           +AGIDT A+TL WALSHLLNNP VL KAK EID +IG+ER+VNE D+ SLSYLQGII+ET
Sbjct: 308 IAGIDTSAVTLQWALSHLLNNPIVLDKAKAEIDSYIGQERMVNEVDLSSLSYLQGIISET 367

Query: 302 LRLNPPAPLLVPHCALFEECKIEGYDVPRDTIILINAWAIHRDPSLWEDATSFKPERHAS 361
           LRL+PP PLLVPHCA  E+CKI GYDVPR+TI+LINAWAIHRDP++WEDA SFKPERH +
Sbjct: 368 LRLSPPGPLLVPHCA-SEDCKIGGYDVPRNTIVLINAWAIHRDPNVWEDAGSFKPERHVN 427

Query: 362 GIEASESYKLIPFGAGRRACPGVGMAQRILAFTLASLIQSFEWER---ALIDMSEGHGLT 421
            +    SYKL+PFG GRRACPG+ MAQR++  TLASLIQ FEW++    L+DM EG GLT
Sbjct: 428 AVGFENSYKLLPFGLGRRACPGMAMAQRVVGLTLASLIQCFEWKKMSNLLVDMREGEGLT 487

Query: 422 MPKAQPLVAKCKPCPILKAIL 429
           MPK + LVAKC+P  I+K +L
Sbjct: 488 MPKVESLVAKCRPRFIMKVVL 506

BLAST of Cla010518 vs. NCBI nr
Match: gi|659111617|ref|XP_008455822.1| (PREDICTED: LOW QUALITY PROTEIN: cytochrome P450 81D1-like [Cucumis melo])

HSP 1 Score: 614.8 bits (1584), Expect = 1.2e-172
Identity = 310/428 (72.43%), Postives = 354/428 (82.71%), Query Frame = 1

Query: 2   SLRFGSRLVVVVSSPSAVKECFTKNDIILANRPLLNTTKCLAYNQTTMGVSPYGEHWRNL 61
           SL+FGSRLVV+VSSPSA++ECFTKNDIILANRP LN+ K LAYN TTM VS YG+HWRNL
Sbjct: 68  SLKFGSRLVVLVSSPSAIQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSSYGKHWRNL 127

Query: 62  RRIGSYEIFSKNRLNFFLGIREDEVKRLLCKLCGNCYKLEDEFRVVELEPMLQDLTSNIV 121
           RRI + EIFS  RLN F   RE+EVKRLL KL GN YKLEDEFRVVELEPML DLT NIV
Sbjct: 128 RRISTLEIFSTTRLNSFSRTREEEVKRLLRKLWGN-YKLEDEFRVVELEPMLLDLTFNIV 187

Query: 122 MRMVCGKKFYEED---------YCSKFRDIVTQILAHGGATNPGDFIPLWNWIDPTGFMK 181
           MRMV GKKF EE          YC +F+++VTQI+AHGG+TNPGDFIPL NWIDP+G+ K
Sbjct: 188 MRMVGGKKFCEEKNNNVLEDEGYCKRFKELVTQIMAHGGSTNPGDFIPLLNWIDPSGYEK 247

Query: 182 RVKKIAKTSDEFLQQLIDEIRNQNDGGDTMIHHLLSLQNIEPQYYNDQTIKGLIQDIFLA 241
            + KI + +DE LQ L+DEIRN+ D G+TMI HLL LQ  +P+YY+DQ IKGL+QDI LA
Sbjct: 248 DIMKIGRKTDEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDPEYYSDQIIKGLVQDIILA 307

Query: 242 GIDTLALTLGWALSHLLNNPEVLKKAKVEIDCWIGEERVVNEADVPSLSYLQGIITETLR 301
           GIDT  ++L WALS LLNNPEVLKKAK EIDC IG +R+V+E D+PSLSYLQGII+ETLR
Sbjct: 308 GIDTSTISLEWALSQLLNNPEVLKKAKEEIDCLIGHKRLVSEVDLPSLSYLQGIISETLR 367

Query: 302 LNPPAPLLVPHCALFEECKIEGYDVPRDTIILINAWAIHRDPSLWEDATSFKPERHASGI 361
           L+PPAPLLVPHCALFEECKI+ Y++PRDTI+LINAWAIHRD  LWEDAT FKPERH+  +
Sbjct: 368 LHPPAPLLVPHCALFEECKIKRYNIPRDTIVLINAWAIHRDSGLWEDATEFKPERHS--V 427

Query: 362 EASESYKLIPFGAGRRACPGVGMAQRILAFTLASLIQSFEWER---ALIDMSEGHGLTMP 418
             SE YKLIPFG GRRACPG GMAQR++   LASLIQSFEWER   +LIDMSEG GLTMP
Sbjct: 428 IESELYKLIPFGVGRRACPGAGMAQRVVGLALASLIQSFEWERVDDSLIDMSEGKGLTMP 487

BLAST of Cla010518 vs. NCBI nr
Match: gi|659111619|ref|XP_008455823.1| (PREDICTED: cytochrome P450 81D1-like [Cucumis melo])

HSP 1 Score: 595.9 bits (1535), Expect = 5.7e-167
Identity = 306/442 (69.23%), Postives = 355/442 (80.32%), Query Frame = 1

Query: 1   MSLRFGSRLVVVVSSPSAVKECFTKNDIILANRPLLNTTKCLAYNQTTMGVSPYGEHWRN 60
           +SLRFGS L VVVSS S V+ECFTKND++LANRPLL + K L YN T + +SPYGEHWRN
Sbjct: 67  LSLRFGSCLAVVVSSSSLVQECFTKNDVVLANRPLLKSAKHLTYNYTNLAISPYGEHWRN 126

Query: 61  LRRIGSYEIFSKNRLNFFLGIREDEVKRLLCKLCGNCYKLEDEFRVVE-LEPMLQDLTSN 120
           LRRI + EI S +RLN F+G+REDEVKRLLCKL    Y  E EF+VV+ + PM  DL  N
Sbjct: 127 LRRISTLEILSLSRLNLFMGVREDEVKRLLCKLWDG-YSFE-EFKVVDQMLPMFMDLMFN 186

Query: 121 IVMRMVCGKKFY-----EEDYCSKFRDIVTQILAHGGATNPGDFIPLWNWIDPTGFMKRV 180
           IVMRM+CGKK+      +E+   KF+++V Q+LA  GA NPGDFIPLWNWIDPT + KRV
Sbjct: 187 IVMRMMCGKKYCGDDLKDEERSKKFKEMVKQVLAISGAGNPGDFIPLWNWIDPTRYEKRV 246

Query: 181 KKIAKTSDEFLQQLIDEIRNQNDGGDTMIHHLLSLQNIEPQYYNDQTIKGLIQDIFLAGI 240
           KK+ KTSDE +QQLIDEIRNQNDG +TMI HLL LQN +P+ Y+DQ IKGLIQ I L GI
Sbjct: 247 KKLTKTSDELIQQLIDEIRNQNDGENTMIQHLLGLQNTQPENYSDQIIKGLIQVILLGGI 306

Query: 241 DTLALTLGWALSHLLNNPEVLKKAKVEIDCWIGEERVVNEADVPSLSYLQGIITETLRLN 300
           DT A+TL WALSHLLNNPEVLKKA  EID  IG+E +V E D  SLSYLQGII+ETLRLN
Sbjct: 307 DTAAVTLEWALSHLLNNPEVLKKAIDEIDSSIGQECLVKEVDSFSLSYLQGIISETLRLN 366

Query: 301 PPAPLLVPHCALFEECKIEGYDVPRDTIILINAWAIHRDPSLWEDATSFKPERH--ASGI 360
           P APLLVPHCA FE+CKI GYDVPRDTI+LINAW IHRDPSLWEDATSFKPERH  A+G+
Sbjct: 367 PAAPLLVPHCA-FEDCKIGGYDVPRDTIVLINAWTIHRDPSLWEDATSFKPERHENANGV 426

Query: 361 EASESYKLIPFGAGRRACPGVGMAQRILAFTLASLIQSFEWER---ALIDMSEGHGLTMP 420
           +A   YKL+PFG GRRACPG+GMAQR++A TLASLIQ FEW+R   +L+DM+EG GLTMP
Sbjct: 427 DA---YKLLPFGLGRRACPGMGMAQRVVALTLASLIQCFEWQRLGNSLVDMTEGEGLTMP 486

Query: 421 KAQPLVAKCKPCPILKAILKAR 432
           KAQPL AKC+P PI+K IL  +
Sbjct: 487 KAQPLTAKCRPRPIMKKILSMK 502

BLAST of Cla010518 vs. NCBI nr
Match: gi|778673620|ref|XP_011650030.1| (PREDICTED: cytochrome P450 81D1-like [Cucumis sativus])

HSP 1 Score: 590.5 bits (1521), Expect = 2.4e-165
Identity = 294/437 (67.28%), Postives = 354/437 (81.01%), Query Frame = 1

Query: 2   SLRFGSRLVVVVSSPSAVKECFTKNDIILANRPLLNTTKCLAYNQTTMGVSPYGEHWRNL 61
           SLRFGS LVVV+SSPSAV+ECFTKNDIILANRP LNT K L+YN T + V+PYGE WR L
Sbjct: 67  SLRFGSHLVVVISSPSAVRECFTKNDIILANRPFLNTGKHLSYNFTVLAVAPYGELWRRL 126

Query: 62  RRIGSYEIFSKNRLNFFLGIREDEVKRLLCKLCGNCYKLEDE--FRVVELEPMLQDLTSN 121
           RRI + EIFS ++LN F  IR+DEVKRLL KLC N   +E+   F  VELEP+L D TSN
Sbjct: 127 RRISTCEIFSTSKLNSFSCIRQDEVKRLLHKLC-NRNSVEELLLFSAVELEPILLDFTSN 186

Query: 122 IVMRMVCGKKFYEEDY-----CSKFRDIVTQILAHGGATNPGDFIPLWNWIDPTGFMKRV 181
           IVMRMV GK+++ +D        KFRD+V +++ + GATNPGDFIPLWNWIDPTG  K++
Sbjct: 187 IVMRMVGGKRYFGDDVLDEGQAEKFRDVVKRVMLYAGATNPGDFIPLWNWIDPTGLEKKI 246

Query: 182 KKIAKTSDEFLQQLIDEIRNQNDGGDTMIHHLLSLQNIEPQYYNDQTIKGLIQDIFLAGI 241
            K+ + +DE  Q LIDEIRN+ + G+TMIHHLL LQN +P+Y++DQ IKGLI  IFLAGI
Sbjct: 247 MKVGEEADEIFQGLIDEIRNEEEDGNTMIHHLLHLQNTQPEYFSDQIIKGLIHVIFLAGI 306

Query: 242 DTLALTLGWALSHLLNNPEVLKKAKVEIDCWIGEERVVNEADVPSLSYLQGIITETLRLN 301
           DT+++TL W LSHLLNNP+V+KKA++EI+  +G+ER+VNE D+ SLSYLQGII ETLRL 
Sbjct: 307 DTISVTLEWGLSHLLNNPKVIKKARLEIEHIVGQERLVNEDDLSSLSYLQGIILETLRLT 366

Query: 302 PPAPLLVPHCALFEECKIEGYDVPRDTIILINAWAIHRDPSLWEDATSFKPERHASGIEA 361
           P APLLVPHCA  E+C+IEGYD+PR TII +NAWAIHRD SLWED TSFKPERH + IE 
Sbjct: 367 PAAPLLVPHCA-SEDCQIEGYDIPRGTIIFVNAWAIHRDSSLWEDVTSFKPERHENAIEL 426

Query: 362 SESYKLIPFGAGRRACPGVGMAQRILAFTLASLIQSFEWER---ALIDMSEGHGLTMPKA 421
           S+SYKL+PFG GRRACPGVGMAQR+L  TLASLIQ F+WER   +L+DM+EG G+TMPKA
Sbjct: 427 SDSYKLLPFGLGRRACPGVGMAQRVLGLTLASLIQCFDWERMDSSLVDMTEGQGITMPKA 486

Query: 422 QPLVAKCKPCPILKAIL 429
           QPLVAKCKP PI++A L
Sbjct: 487 QPLVAKCKPRPIMRAHL 501

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
C81E9_MEDTR2.1e-12153.06Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula GN=CYP81E9 PE=1 SV=1[more]
C81E1_GLYEC6.4e-11850.46Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata GN=CYP81E1 PE=1 SV=2[more]
C8D11_ARATH1.3e-11552.05Cytochrome P450 81D11 OS=Arabidopsis thaliana GN=CYP81D11 PE=2 SV=1[more]
C81E7_MEDTR3.9e-11549.89Isoflavone 2'-hydroxylase OS=Medicago truncatula GN=CYP81E7 PE=1 SV=1[more]
C81E8_MEDTR1.9e-11451.87Cytochrome P450 81E8 OS=Medicago truncatula GN=CYP81E8 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LQI5_CUCSA1.7e-17369.39Uncharacterized protein OS=Cucumis sativus GN=Csa_2G423640 PE=3 SV=1[more]
A0A0A0LTD0_CUCSA1.7e-16567.28Uncharacterized protein OS=Cucumis sativus GN=Csa_2G425740 PE=3 SV=1[more]
A0A0A0LNA4_CUCSA6.5e-16266.59Uncharacterized protein OS=Cucumis sativus GN=Csa_2G425750 PE=3 SV=1[more]
A0A061EHP2_THECC1.8e-13558.03Cytochrome P450, family 81, subfamily D, polypeptide 8, putative OS=Theobroma ca... [more]
A0A0A0KJM0_CUCSA8.9e-13558.96Cytochrome P450 OS=Cucumis sativus GN=Csa_6G492230 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
gi|659111621|ref|XP_008455824.1|3.5e-18574.19PREDICTED: cytochrome P450 81D1-like [Cucumis melo][more]
gi|778674449|ref|XP_004151924.2|2.4e-17369.39PREDICTED: cytochrome P450 81D1-like [Cucumis sativus][more]
gi|659111617|ref|XP_008455822.1|1.2e-17272.43PREDICTED: LOW QUALITY PROTEIN: cytochrome P450 81D1-like [Cucumis melo][more]
gi|659111619|ref|XP_008455823.1|5.7e-16769.23PREDICTED: cytochrome P450 81D1-like [Cucumis melo][more]
gi|778673620|ref|XP_011650030.1|2.4e-16567.28PREDICTED: cytochrome P450 81D1-like [Cucumis sativus][more]
The following terms have been associated with this mRNA:
Vocabulary: INTERPRO
TermDefinition
IPR001128Cyt_P450
IPR002401Cyt_P450_E_grp-I
IPR017972Cyt_P450_CS
Vocabulary: Molecular Function
TermDefinition
GO:0016705oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
GO:0005506iron ion binding
GO:0020037heme binding
Vocabulary: Biological Process
TermDefinition
GO:0055114oxidation-reduction process
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055114 oxidation-reduction process
cellular_component GO:0005575 cellular_component
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
molecular_function GO:0004497 monooxygenase activity

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Cla010518Cla010518gene


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Cla010518Cla010518.1-proteinpolypeptide


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cla010518.1.cds1Cla010518.1.cds1CDS
Cla010518.1.cds2Cla010518.1.cds2CDS


Analysis Name: InterPro Annotations of watermelon (97103)
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001128Cytochrome P450PRINTSPR00385P450coord: 361..370
score: 8.4E-11coord: 285..296
score: 8.4E-11coord: 370..381
score: 8.4E-11coord: 232..249
score: 8.4
IPR001128Cytochrome P450GENE3DG3DSA:1.10.630.10coord: 1..420
score: 6.5E
IPR001128Cytochrome P450PFAMPF00067p450coord: 2..394
score: 3.0
IPR001128Cytochrome P450unknownSSF48264Cytochrome P450coord: 2..420
score: 9.04E
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 284..302
score: 1.0E-41coord: 370..393
score: 1.0E-41coord: 326..350
score: 1.0E-41coord: 18..39
score: 1.0E-41coord: 221..238
score: 1.0E-41coord: 116..134
score: 1.0E-41coord: 241..267
score: 1.0E-41coord: 360..370
score: 1.0
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 363..372
scor
NoneNo IPR availablePANTHERPTHR24298FAMILY NOT NAMEDcoord: 1..425
score: 1.3E
NoneNo IPR availablePANTHERPTHR24298:SF61CYTOCHROME P450 81D1-RELATEDcoord: 1..425
score: 1.3E