Cla003209.1 (mRNA) Watermelon (97103) v1

BLAST of Cla003209 vs. Swiss-Prot
Match: LOX21_SOLTU (Linoleate 13S-lipoxygenase 2-1, chloroplastic OS=Solanum tuberosum GN=LOX2.1 PE=1 SV=1)

HSP 1 Score: 681.8 bits (1758), Expect = 9.7e-195
Identity = 380/812 (46.80%), Postives = 492/812 (60.59%), Query Frame = 1

Query: 66  YEGKIEVQDGFGRIGAVIVKISKDNSSTETFIDTVTIQKSMSDNLITFSCKSWVQPKNDD 125
           YE    +   FG +GA++++   +    E ++  + I   +    +  +C SWV  K D+
Sbjct: 137 YEADFVIPQDFGEVGAILIE---NEHHKEMYVKNIVIDGFVHGK-VEITCNSWVHSKFDN 196

Query: 126 DDDDEYSQRRIFFSTKSYLPDNTPAGLVKLRAEDLRNLRGEKPDGTIDMKERKENERIYD 185
            D      +RIFF+ KSYLP  TP+G+ +LR E+L  LRG+       + ERK  ERIYD
Sbjct: 197 PD------KRIFFTNKSYLPSQTPSGVSRLREEELVTLRGD------GIGERKVFERIYD 256

Query: 186 YDVYNDLGETD-DVPMSKRHVFGGSKEYPYPRRYDSFETRG---------SGKFYVPRDE 245
           YDVYNDLGE D +   +KR V GG KE PYPRR  +   R          S   YVPRDE
Sbjct: 257 YDVYNDLGEADSNNDDAKRPVLGG-KELPYPRRCKTGRPRSKKDPLSETRSTFVYVPRDE 316

Query: 246 EFSKVKQSYFPDMGQKNNMDELGEDHFTDLREIESLYHGGIKTPTAPHKILHLEISSILS 305
            FS+VK   F            G   ++ L  +       +  P            ++  
Sbjct: 317 AFSEVKSVAFS-----------GNTVYSVLHAVVPALESVVTDP------------NLGF 376

Query: 306 PHQPPVLHSAISLTTILPPAESHISEYTFYINETLKSVKITVHLPHICLLIFP----RDR 365
           PH P +  S  ++   LP      S     +   +K++  T     + L   P    RD+
Sbjct: 377 PHFPAI-DSLFNVGVDLPGLGDKKSGLFNVVPRLIKAISDT--RKDVLLFESPQLVQRDK 436

Query: 366 YNWLSDGEFARQTLAGVNPCSIQLVKSLPFESALDIHKYGPKESKFTPTWFEESMKGSIT 425
           ++W  D EFARQTLAG+NP SI+LV   P  S LD   YGP ES+ T    E+ +   +T
Sbjct: 437 FSWFRDVEFARQTLAGLNPYSIRLVTEWPLRSKLDPKVYGPPESEITKELIEKEIGNYMT 496

Query: 426 YIQAITQKKLFVLDYHDILMPYVIDVRNIDNKTLYGSRTLFFLNSDDTLMPLGIELTRPP 485
             QA+ QKKLF+LDYHD+L+PYV  V  +    LYGSRT+FFL    TL PL IELTRPP
Sbjct: 497 VEQAVQQKKLFILDYHDLLLPYVNKVNELKGSMLYGSRTIFFLTPQGTLKPLAIELTRPP 556

Query: 486 MDGDGQWKEIFKPSI-KGTDLWQWRFAKAHVLSHDSCIHQLVIHWLRAHCCMETYAIATN 545
           +D   QWKE++ P+    T  W W+ AKAHVLSHDS  HQLV HWLR HCC E Y IA+N
Sbjct: 557 VDDKPQWKEVYSPNDWNATGAWLWKLAKAHVLSHDSGYHQLVSHWLRTHCCTEPYIIASN 616

Query: 546 RQLSTMHPIYRLLHPHFRYNMRINAQARKDLISAGGLIEGTFSTTSLSMELSSLVYKNEW 605
           RQLS MHPIYRLLHPHFRY M INA AR+ LI+A G+IE +F     ++ELSS+ Y  EW
Sbjct: 617 RQLSAMHPIYRLLHPHFRYTMEINALAREALINANGVIESSFFPGKYAIELSSIAYGAEW 676

Query: 606 QFDQQALPNDLIRRGMAKQKKDEEGHDVLELAIKDYPFANDGLILWNAILDWVKDYVEHY 665
           +FDQ+ALP +LI RG+A +  +E     L+LAI+DYPFANDGL+LW+ +  WV +YV HY
Sbjct: 677 RFDQEALPQNLISRGLAVEDPNEP--HGLKLAIEDYPFANDGLVLWDILKQWVTNYVNHY 736

Query: 666 YQGDKGNKLVKNDKELQAWWTEIKEKGHPDKKE--GWPKLETKEDLIKIVSTIAWVGSGH 725
           Y       L+++DKELQAWW+EIK  GH DK++   WP+L+T  DLI I++TI WV SGH
Sbjct: 737 Y---PQTNLIESDKELQAWWSEIKNVGHGDKRDEPWWPELKTPNDLIGIITTIVWVTSGH 796

Query: 726 HSTVNFIQYAYGGFIPNRPSIARINVPSEDSSKK----FINQPEEVLLKIFPSAAQGTAV 785
           H+ VNF QY+Y G+ PNRP++AR  +P+ED + +    F+N+PEE LL+ FPS  Q T V
Sbjct: 797 HAAVNFGQYSYAGYFPNRPTVARSKMPTEDPTAEEWEWFMNKPEEALLRCFPSQIQATKV 856

Query: 786 ATAMALLSTHSSDEQYIGEDIKIELAWDHDNKIKVAFEKFCRKLKQLEGTIDDNNKDSEK 845
              + +LS HS DE+YIGE  KIE  W  D  I  AFE F  KLK+LEG ID  N DS+ 
Sbjct: 857 MAILDVLSNHSPDEEYIGE--KIEPYWAEDPVINAAFEVFSGKLKELEGIIDARNNDSKL 898

Query: 846 KNRSGAGVIPYDVLKPYSKSGITGMGVPYSVS 857
            NR+GAGV+PY++LKPYS+ G+TG GVPYS+S
Sbjct: 917 SNRNGAGVMPYELLKPYSEPGVTGKGVPYSIS 898

BLAST of Cla003209 vs. Swiss-Prot
Match: LOX2_ARATH (Lipoxygenase 2, chloroplastic OS=Arabidopsis thaliana GN=LOX2 PE=1 SV=1)

HSP 1 Score: 674.1 bits (1738), Expect = 2.0e-192
Identity = 375/811 (46.24%), Postives = 494/811 (60.91%), Query Frame = 1

Query: 66  YEGKIEVQDGFGRIGAVIVKISKDNSSTETFIDTVTIQKSMSDNLITFSCKSWVQPKNDD 125
           YE + E+ + FG +GA+ ++   +    + F+  V ++  +    ITF+C+SWV PK+ D
Sbjct: 137 YECEFEMPEDFGPVGAIKIQ---NQYHRQLFLKGVELK--LPGGSITFTCESWVAPKSVD 196

Query: 126 DDDDEYSQRRIFFSTKSYLPDNTPAGLVKLRAEDLRNLRGEKPDGTIDMKERKENERIYD 185
                   +RIFFS KSYLP  TP  L K R E+L  L+G+  +   ++ E  + ERIYD
Sbjct: 197 PT------KRIFFSDKSYLPSQTPEPLKKYRKEELETLQGKNRE---EVGEFTKFERIYD 256

Query: 186 YDVYNDLGETDDVPMSKRHVFGGSKEYPYPRRYD----------SFETRGSGKFYVPRDE 245
           YDVYND+G+ D+ P   R V GG   +PYPRR            S E R  G+FYVPRDE
Sbjct: 257 YDVYNDVGDPDNDPELARPVIGGLT-HPYPRRCKTGRKPCETDPSSEQRYGGEFYVPRDE 316

Query: 246 EFSKVKQSYFPDMGQKNNMDELGEDHFTDLREIESLYHGGIKTPTAPHK----ILHLEIS 305
           EFS  K + F        +  +         +IES+    + +P  P      I +L   
Sbjct: 317 EFSTAKGTSFTGKAVLAALPSI-------FPQIESV----LLSPQEPFPHFKAIQNLFEE 376

Query: 306 SILSPHQPPVLHSAISLTTILPPAESHISEYTFYINETLKSVKITVHLPHICLLIFPRDR 365
            I  P    +L     +   L  A+  I ++   +                   +  RDR
Sbjct: 377 GIQLPKDAGLLPLLPRIIKALGEAQDDILQFDAPV-------------------LINRDR 436

Query: 366 YNWLSDGEFARQTLAGVNPCSIQLVKSLPFESALDIHKYGPKESKFTPTWFEESMKGSIT 425
           ++WL D EFARQTLAG+NP SIQLV+  P  S LD   YG   S  T    E  +KG++T
Sbjct: 437 FSWLRDDEFARQTLAGLNPYSIQLVEEWPLISKLDPAVYGDPTSLITWEIVEREVKGNMT 496

Query: 426 YIQAITQKKLFVLDYHDILMPYVIDVRNIDNKTLYGSRTLFFLNSDDTLMPLGIELTRPP 485
             +A+  K+LFVLDYHD+L+PYV  VR ++N TLY SRTLFFL+ D TL P+ IELT PP
Sbjct: 497 VDEALKNKRLFVLDYHDLLLPYVNKVRELNNTTLYASRTLFFLSDDSTLRPVAIELTCPP 556

Query: 486 MDGDGQWKEIFKPSIKGTDLWQWRFAKAHVLSHDSCIHQLVIHWLRAHCCMETYAIATNR 545
                QWK++F P    T  W W  AK H +SHD+  HQL+ HWLR H C E Y IA NR
Sbjct: 557 NINKPQWKQVFTPGYDATSCWLWNLAKTHAISHDAGYHQLISHWLRTHACTEPYIIAANR 616

Query: 546 QLSTMHPIYRLLHPHFRYNMRINAQARKDLISAGGLIEGTFSTTSLSMELSSLVYKNEWQ 605
           QLS MHPIYRLLHPHFRY M INA+AR+ L++ GG+IE  F     ++ELSS VY   W+
Sbjct: 617 QLSAMHPIYRLLHPHFRYTMEINARARQSLVNGGGIIETCFWPGKYALELSSAVYGKLWR 676

Query: 606 FDQQALPNDLIRRGMAKQKKDEEGHDVLELAIKDYPFANDGLILWNAILDWVKDYVEHYY 665
           FDQ+ LP DLI+RG+A++ K  E H V  L I DYPFANDGLILW+AI +WV DYV+HYY
Sbjct: 677 FDQEGLPADLIKRGLAEEDKTAE-HGV-RLTIPDYPFANDGLILWDAIKEWVTDYVKHYY 736

Query: 666 QGDKGNKLVKNDKELQAWWTEIKEKGHPDKKE--GWPKLETKEDLIKIVSTIAWVGSGHH 725
             +   +L+ +D+ELQ WW+E++  GH DKK+   WP L+T++DLI +V+TIAWV SGHH
Sbjct: 737 PDE---ELITSDEELQGWWSEVRNIGHGDKKDEPWWPVLKTQDDLIGVVTTIAWVTSGHH 796

Query: 726 STVNFIQYAYGGFIPNRPSIARINVPSEDSS----KKFINQPEEVLLKIFPSAAQGTAVA 785
           + VNF QY YGG+ PNRP+  RI +P+ED +    K+F   PE+VLLK +PS  Q T V 
Sbjct: 797 AAVNFGQYGYGGYFPNRPTTTRIRMPTEDPTDEALKEFYESPEKVLLKTYPSQKQATLVM 856

Query: 786 TAMALLSTHSSDEQYIGEDIKIELAWDHDNKIKVAFEKFCRKLKQLEGTIDDNNKDSEKK 845
             + LLSTHS DE+YIGE  + E +W ++  I  AFE+F  KL+ LEG ID+ N +   K
Sbjct: 857 VTLDLLSTHSPDEEYIGE--QQEASWANEPVINAAFERFKGKLQYLEGVIDERNVNITLK 895

Query: 846 NRSGAGVIPYDVLKPYSKSGITGMGVPYSVS 857
           NR+GAGV+ Y++LKP S+ G+TGMGVPYS+S
Sbjct: 917 NRAGAGVVKYELLKPTSEHGVTGMGVPYSIS 895

BLAST of Cla003209 vs. Swiss-Prot
Match: LOX23_HORVU (Lipoxygenase 2.3, chloroplastic OS=Hordeum vulgare GN=LOX2.3 PE=1 SV=1)

HSP 1 Score: 661.4 bits (1705), Expect = 1.4e-188
Identity = 367/817 (44.92%), Postives = 479/817 (58.63%), Query Frame = 1

Query: 65  TYEGKIEVQDGFGRIGAVIVKISKDNSSTETFI-DTVTIQKSMSDNLITFSCKSWVQPKN 124
           TYE K+ V   FG +GAV+V+   +    E FI D   I        ITF   SWV  K 
Sbjct: 133 TYEAKMSVPASFGPVGAVLVE---NEHHREMFIKDIKLITGGDESTAITFDVASWVHSKF 192

Query: 125 DDDDDDEYSQRRIFFSTKSYLPDNTPAGLVKLRAEDLRNLRGEKPDGTIDMKERKENERI 184
           DD +       R FF+ KSYLP  TP G+  LR ++L  LRG+         ERK +ER+
Sbjct: 193 DDPEP------RAFFTVKSYLPSQTPPGIEALRKKELETLRGD------GHSERKFHERV 252

Query: 185 YDYDVYNDLGETDDVPMSKRHVFGGSKEYPYPRR---------YDSFETRGSGKFYVPRD 244
           YDYD YNDLG+ D     KR V  G+KE+PYPRR         YD      S   YVPRD
Sbjct: 253 YDYDTYNDLGDPDKNIDHKRPVL-GTKEHPYPRRCRTGRPKTLYDPETETRSSPVYVPRD 312

Query: 245 EEFSKVKQSYFPDMGQKNNMDE--------LGEDH-FTDLREIESLYHGGIKTPTAPHKI 304
           E+FS VK   F     ++ +          L   H F+    I++LY  GI  P   H  
Sbjct: 313 EQFSDVKGRTFSATTLRSGLHAILPAVAPLLNNSHGFSHFPAIDALYSDGIPLPVDGHGG 372

Query: 305 LHLEISSILSPHQPPVLHSAISLTTILPPAESHISEYTFYINETLKSVKITVHLPHICLL 364
               +                 +  ++P     I + T ++        +   +P     
Sbjct: 373 NSFNV-----------------INDVIPRVVQMIEDTTEHV--------LRFEVPE---- 432

Query: 365 IFPRDRYNWLSDGEFARQTLAGVNPCSIQLVKSLPFESALDIHKYGPKESKFTPTWFEES 424
           +  RDR++W  D EFARQTLAG+NP  I+ +   P  S LD   YGP ES  +    E+ 
Sbjct: 433 MLERDRFSWFRDEEFARQTLAGLNPICIRRLTEFPIVSKLDPAVYGPAESALSKEILEKM 492

Query: 425 MKGSITYIQAITQKKLFVLDYHDILMPYVIDVRNIDNKTLYGSRTLFFLNSDDTLMPLGI 484
           M G +T  +A+ +K+LF+LDYHD+ +PYV  VR + + TLYGSRT+FFL+ + TLMPL I
Sbjct: 493 MNGRMTVEEAMEKKRLFLLDYHDVFLPYVHRVRELPDTTLYGSRTVFFLSDEGTLMPLAI 552

Query: 485 ELTRPPMDGDGQWKEIFKPSIKGTDLWQWRFAKAHVLSHDSCIHQLVIHWLRAHCCMETY 544
           ELTRP      QWK  F      T+ W W+ AKAHVL+HD+  HQLV HWLR H C+E Y
Sbjct: 553 ELTRPQSPTKPQWKRAFTHGSDATESWLWKLAKAHVLTHDTGYHQLVSHWLRTHACVEPY 612

Query: 545 AIATNRQLSTMHPIYRLLHPHFRYNMRINAQARKDLISAGGLIEGTFSTTSLSMELSSLV 604
            IATNRQLS MHP+YRLLHPHFRY M INA AR+ LI+A G+IE  F     S+ELSS+ 
Sbjct: 613 IIATNRQLSRMHPVYRLLHPHFRYTMEINALAREALINADGIIEEAFLAGKYSIELSSVA 672

Query: 605 YKNEWQFDQQALPNDLIRRGMAKQKKDEEGHDVLELAIKDYPFANDGLILWNAILDWVKD 664
           Y   WQF+ +ALP DLI RG+A ++ D E    LELAIKDYP+A+DGL++W +I  W  D
Sbjct: 673 YGAAWQFNTEALPEDLINRGLAVRRDDGE----LELAIKDYPYADDGLLIWGSIKQWASD 732

Query: 665 YVEHYYQGDKGNKLVKNDKELQAWWTEIKEKGHPDKKE--GWPKLETKEDLIKIVSTIAW 724
           YV+ YY+ D     V  D+EL+AWW E++ KGH DKK+   WP  +TKE+L++I++ I W
Sbjct: 733 YVDFYYKSDGD---VAGDEELRAWWEEVRTKGHADKKDEPWWPVCDTKENLVQILTIIMW 792

Query: 725 VGSGHHSTVNFIQYAYGGFIPNRPSIARINVPSE----DSSKKFINQPEEVLLKIFPSAA 784
           V SGHH+ VNF QY Y G+ PNRP++ R N+P E    D  KKF+ +PEEVLL+  PS  
Sbjct: 793 VTSGHHAAVNFGQYHYAGYFPNRPTVVRRNIPVEENRDDEMKKFMARPEEVLLQSLPSQM 852

Query: 785 QGTAVATAMALLSTHSSDEQYIGEDIKIELAWDHDNKIKVAFEKFCRKLKQLEGTIDDNN 844
           Q   V   + +LS+HS DE+Y+GE    E AW  +  +K AFEKF  +LK+ EGTID  N
Sbjct: 853 QAIKVMATLDILSSHSPDEEYMGE--YAEPAWLAEPMVKAAFEKFSGRLKEAEGTIDMRN 895

Query: 845 KDSEKKNRSGAGVIPYDVLKPYSKSGITGMGVPYSVS 857
            + E KNR GAG++PY++LKP+S+ G+TG G+P S+S
Sbjct: 913 NNPENKNRCGAGIVPYELLKPFSEPGVTGRGIPNSIS 895

BLAST of Cla003209 vs. Swiss-Prot
Match: LOXC1_ORYSJ (Lipoxygenase 7, chloroplastic OS=Oryza sativa subsp. japonica GN=CM-LOX1 PE=2 SV=2)

HSP 1 Score: 649.0 bits (1673), Expect = 7.0e-185
Identity = 358/826 (43.34%), Postives = 486/826 (58.84%), Query Frame = 1

Query: 65  TYEGKIEVQDGFGRIGAVIVKISKDNSSTETFIDTVTIQKSM---SDNLITFSCKSWVQP 124
           TYE   +V  GFG IGA+IV    +    E F++ + +  S    +  ++   C SWVQP
Sbjct: 150 TYEADFDVPSGFGPIGAIIVT---NELRQEMFLEDINLTASDGAGNSTVLPIRCNSWVQP 209

Query: 125 KNDDDDDDEYSQRRIFFSTKSYLPDNTPAGLVKLRAEDLRNLRGEKPDGTIDMKERKENE 184
           K+  D+      +RIFF+ K+YLP  TPAGL   R  DL+  RG   DGT    ER+ ++
Sbjct: 210 KSVGDEGTP--SKRIFFANKTYLPGQTPAGLRSYRKNDLQQKRG---DGT---GEREADD 269

Query: 185 RIYDYDVYNDLGETDDVPMSKRHVFGGSKEYPYPRRY----------DSFETRGSGKFYV 244
           R+YDYDVYNDLG  D      R V GG+K++PYPRR              ETR  G  YV
Sbjct: 270 RVYDYDVYNDLGNPDSNGDLARPVLGGNKQFPYPRRCRTGRPPSKKDPKSETR-KGNVYV 329

Query: 245 PRDEEFSKVKQSYF------------PDMGQKNNMDELGED-HFTDLREIESLYHGGIKT 304
           PRDEEFS  K+ YF                Q   +D+L  +  F     I+ L+  G++ 
Sbjct: 330 PRDEEFSPEKEDYFLRKTVGSVLQAAVPAAQSLLLDKLKWNLPFPSFFVIDKLFEDGVEL 389

Query: 305 PTAPH-KILHLEISSILSPHQPPVLHSAISLTTILPPAESHISEYTFYINETLKSVKITV 364
           P       L   +  +L   +       +   T   PA     ++ +             
Sbjct: 390 PGVDKLNFLESVVPRLLEHLRDTPAEKILRFET---PANIQKDKFAW------------- 449

Query: 365 HLPHICLLIFPRDRYNWLSDGEFARQTLAGVNPCSIQLVKSLPFESALDIHKYGPKESKF 424
                            L D EFAR+TLAG+NP +I+LV+  P +S LD   YGP ES  
Sbjct: 450 -----------------LRDEEFARETLAGINPYAIELVREFPLKSKLDPAVYGPAESAI 509

Query: 425 TPTWFEESMKGSITYIQAITQKKLFVLDYHDILMPYVIDVRNIDNKTLYGSRTLFFLNSD 484
           T    EE M+  +T  +AI+QK+LF+LD+HD+ +PYV  +R++D+ T+YGSRT+FFL  D
Sbjct: 510 TADLLEEQMRRVMTVEEAISQKRLFMLDFHDLFLPYVHKIRSLDHTTMYGSRTVFFLTDD 569

Query: 485 DTLMPLGIELTRPPMDGDGQWKEIFKPSIKGTDLWQWRFAKAHVLSHDSCIHQLVIHWLR 544
            TL  L IELTRP      QW+++F PS   T  W WR AKAHV +HD+  H+L+ HWLR
Sbjct: 570 GTLQLLAIELTRPASPSQPQWRQVFTPSTDATMSWLWRMAKAHVRAHDAGHHELITHWLR 629

Query: 545 AHCCMETYAIATNRQLSTMHPIYRLLHPHFRYNMRINAQARKDLISAGGLIEGTFSTTSL 604
            HC +E Y IA NRQLS MHPIY+LL PHFRY MRINA+AR  LISAGG+IE +FS    
Sbjct: 630 THCAVEPYIIAANRQLSEMHPIYQLLRPHFRYTMRINARARSALISAGGIIERSFSPQKY 689

Query: 605 SMELSSLVYKNEWQFDQQALPNDLIRRGMAKQKKDEEGHDVLELAIKDYPFANDGLILWN 664
           SMELSS+ Y   W+FD +ALP DL+RRGMA++  D      L+LAI+DYPFANDGL++W+
Sbjct: 690 SMELSSVAYDKLWRFDTEALPADLVRRGMAEE--DPTAEHGLKLAIEDYPFANDGLLIWD 749

Query: 665 AILDWVKDYVEHYYQGDKGNKLVKNDKELQAWWTEIKEKGHPDKKEG--WPKLETKEDLI 724
           AI  WV+ YV  +Y        V  D+ELQA+WTE++ KGH DKK+   WPKL++ E L 
Sbjct: 750 AIKTWVQAYVARFYPDADS---VAGDEELQAFWTEVRTKGHGDKKDAPWWPKLDSPESLA 809

Query: 725 KIVSTIAWVGSGHHSTVNFIQYAYGGFIPNRPSIARINVPSED-----SSKKFINQPEEV 784
             ++TI WV + HH+ VNF QY +GG+ PNRPSIAR  +P E+     + ++F++ P++ 
Sbjct: 810 HTLTTIVWVAAAHHAAVNFGQYDFGGYFPNRPSIARTVMPVEEPVDGAAMERFLDNPDQA 869

Query: 785 LLKIFPSAAQGTAVATAMALLSTHSSDEQYIGEDIKIELAWDHDNKIKVAFEKFCRKLKQ 844
           L + FPS  Q T V   + +LS+HS+DE+Y+G +      W+ D  ++ A++ F  +LK+
Sbjct: 870 LRECFPSQVQATVVMAVLDVLSSHSTDEEYLGGE--QTRPWNSDAAVQAAYDGFAARLKE 923

Query: 845 LEGTIDDNNKDSEKKNRSGAGVIPYDVLKPYSKSGITGMGVPYSVS 857
           +EG ID  NKD + KNR GAG++PY ++KP+S SG+TGMG+P S S
Sbjct: 930 IEGVIDGRNKDRKLKNRCGAGILPYQLMKPFSDSGVTGMGIPNSTS 923

BLAST of Cla003209 vs. Swiss-Prot
Match: LOXC2_ORYSJ (Probable lipoxygenase 8, chloroplastic OS=Oryza sativa subsp. japonica GN=CM-LOX2 PE=2 SV=1)

HSP 1 Score: 649.0 bits (1673), Expect = 7.0e-185
Identity = 367/857 (42.82%), Postives = 507/857 (59.16%), Query Frame = 1

Query: 29  TIFLTFVSETSHSCEKDNVVEGTVTIKKKKPGEDYN---TYEGKIEVQDGFGRIGAVIVK 88
           ++FL  VS    +  K    + TV     K  +D +   TYE   +V  GFG IGAV+V 
Sbjct: 131 SLFLELVSSELEA--KTGKKKATVHSYAHKVDDDDHGVVTYEADFDVPTGFGPIGAVVVT 190

Query: 89  ISKDNSSTETFIDTVTIQK---SMSDNLITFSCKSWVQPKNDDDDDDEYSQRRIFFSTKS 148
              +    E F++ + +     + +  ++   C SWVQPK+  D+      +RIFF+ K+
Sbjct: 191 ---NELGQEMFLEDLNLTAGDGAGNSTVLPIRCNSWVQPKSSIDEGTP--GKRIFFA-KA 250

Query: 149 YLPDNTPAGLVKLRAEDLRNLRGEKPDGTIDMKERKENERIYDYDVYNDLGETDDVPMSK 208
           YLP  TPAGL   R EDL+  RG          +R+ ++R+YDYDVYNDLG  D      
Sbjct: 251 YLPGQTPAGLRSYREEDLKQKRGNGAG------QREADDRVYDYDVYNDLGNPDSNGDLA 310

Query: 209 RHVFGGSKEYPYPRRYDS----------FETRGSGKFYVPRDEEFSKVKQSYFPDMGQKN 268
           R V GGSK++PYPRR  +           ETR  G  YVPRDEEFS+VK + F       
Sbjct: 311 RPVLGGSKQFPYPRRCRTGRPPSKKDPKSETR-KGNVYVPRDEEFSEVKNAQF------- 370

Query: 269 NMDELGEDHFTDLREIESLYHGGIKTPTAPHKILHLEISSILSPHQPPVLHSAISLTTIL 328
                       L+ ++S+ H  +  P A   ++   +S  L      V+         L
Sbjct: 371 -----------LLKTLQSVLHAAV--PAAQSALID-NLSLNLPFPSFFVIDKLFEDGVEL 430

Query: 329 PPAESHISEYTFYINETLKSVKITVHLPHICLLIFP------RDRYNWLSDGEFARQTLA 388
           P  E    +  F  +   + +++    P   +L+F       +D++ WL D EFAR+TLA
Sbjct: 431 PGVE----KLGFLHSIVPRLLELLRDSPGDKILLFDTPANVQKDKFAWLRDEEFARETLA 490

Query: 389 GVNPCSIQLVKSLPFESALDIHKYGPKESKFTPTWFEESMKGSITYIQAITQKKLFVLDY 448
           G+NP +I+LV+  P +S LD   YGP ES  T    EE M+  +T  +AI+QK+LF+LD+
Sbjct: 491 GINPYAIELVREFPLKSKLDPAVYGPAESAITADLLEEQMRRVMTVEEAISQKRLFMLDF 550

Query: 449 HDILMPYVIDVRNIDNKTLYGSRTLFFLNSDDTLMPLGIELTRPPMDGDGQWKEIFKPSI 508
           HD+ +PYV  +R++ + T+YGSRT+FFL  D TL  L IELTRP      QW+++F PS 
Sbjct: 551 HDLFLPYVHKIRSLKHTTMYGSRTIFFLTDDGTLRLLAIELTRPASPSQPQWRQVFTPST 610

Query: 509 KGTDLWQWRFAKAHVLSHDSCIHQLVIHWLRAHCCMETYAIATNRQLSTMHPIYRLLHPH 568
             T  W WR AKAHV +HD+  H+L+ HWLR HC +E Y IA NRQLS MHPIY+LLHPH
Sbjct: 611 DTTKSWLWRMAKAHVRAHDAGHHELITHWLRTHCAVEPYIIAANRQLSEMHPIYQLLHPH 670

Query: 569 FRYNMRINAQARKDLISAGGLIEGTFSTTSLSMELSSLVYKNEWQFDQQALPNDLIRRGM 628
           FRY MRINA AR  LISA G+IE +FS    SMELSS+ Y   W+FD +ALP DL+RRGM
Sbjct: 671 FRYTMRINALARSRLISAAGIIELSFSPQKYSMELSSVAYDKLWRFDMEALPADLVRRGM 730

Query: 629 AKQKKDEEGHDVLELAIKDYPFANDGLILWNAILDWVKDYVEHYYQGDKGNKLVKNDKEL 688
           A++  D      L LAI+DYPFANDGL++W+AI  WV+ YV  +Y        V  D+EL
Sbjct: 731 AEE--DPTAEHGLRLAIEDYPFANDGLLIWDAIKTWVQAYVARFYPDADS---VAGDEEL 790

Query: 689 QAWWTEIKEKGHPDKKEG--WPKLETKEDLIKIVSTIAWVGSGHHSTVNFIQYAYGGFIP 748
           QA+WTE++ KGH DKK+   WPKL++ E L   ++TI WV + HH+ VNF QY +GG+ P
Sbjct: 791 QAFWTEVRTKGHGDKKDAPWWPKLDSPESLAHTLTTIVWVAAAHHAAVNFGQYDFGGYFP 850

Query: 749 NRPSIARINVPSED-----SSKKFINQPEEVLLKIFPSAAQGTAVATAMALLSTHSSDEQ 808
           NRPSIAR  +P E+     + ++F++ P++ L + FPS  Q T V   + +LSTHS+DE+
Sbjct: 851 NRPSIARTVMPVEEPVDGAAMERFLDNPDQALRECFPSQVQATVVMAVLDVLSTHSTDEE 910

Query: 809 YIGEDIKIELAWDHDNKIKVAFEKFCRKLKQLEGTIDDNNKDSEKKNRSGAGVIPYDVLK 857
           Y+G +      W+ D  ++ A+  F  +LK++EG ID  NKD + KNR GAG++PY ++K
Sbjct: 911 YLGGE--QTRPWNSDAAVQAAYAGFTARLKEIEGVIDGRNKDRKLKNRCGAGILPYQLMK 940

BLAST of Cla003209 vs. TrEMBL
Match: M5W1M2_PRUPE (Lipoxygenase OS=Prunus persica GN=PRUPE_ppa000968mg PE=3 SV=1)

HSP 1 Score: 731.9 bits (1888), Expect = 9.1e-208
Identity = 412/839 (49.11%), Postives = 515/839 (61.38%), Query Frame = 1

Query: 43  EKDNVVEGTVTIKKKKPGEDYNTYEGKIEVQDGFGRIGAVIVKISKDNSSTETFIDTVTI 102
           EK+ V  G     ++K GE    YE   +V   FG +GAV+V+   +    E F+  + +
Sbjct: 169 EKEKVA-GFAHKSRRKEGEII--YETDFKVPVHFGEVGAVLVE---NEHHKEMFLKDIVL 228

Query: 103 QKSMSDNLITFSCKSWVQPKNDDDDDDEYSQRRIFFSTKSYLPDNTPAGLVKLRAEDLRN 162
              +    +  SC SWV  K D+ +      +R+FF+ KSYLP  TP GLV+LR E+L  
Sbjct: 229 D-GLPYGSVHLSCNSWVHSKYDNPE------KRVFFTNKSYLPSQTPNGLVRLREEELVT 288

Query: 163 LRGEKPDGTIDMKERKENERIYDYDVYNDLGETDDVPMSKRHVFGGSKEYPYPRR----- 222
           LRG          ERK  ERIYDYDVYNDLGE D     +R V GG  E+PYPRR     
Sbjct: 289 LRGN------GQGERKFFERIYDYDVYNDLGEPDKNLRLERPVLGGI-EFPYPRRCRTGR 348

Query: 223 ----YDSFETRGSGKFYVPRDEEFSKVKQSYFPDMGQKNNMDEL---------GEDH-FT 282
                DS   + S K+YVPRDE FS+VKQ  F      + M  L           DH F 
Sbjct: 349 PQCETDSLSEKRSNKWYVPRDEAFSEVKQLTFSAKTVYSVMHALVPSLETAMADNDHGFA 408

Query: 283 DLREIESLYHGGIKTPTAPHKILHLEISSILSPHQPPVLHSAISLTTILPPAESHISEYT 342
               I+SL++ GI  P  P K     I   L P    V+ S   +   +PP         
Sbjct: 409 YFTAIDSLFNEGINLP--PFK--EQGILKTLLPRLVNVVASGDDVLRFVPP--------- 468

Query: 343 FYINETLKSVKITVHLPHICLLIFPRDRYNWLSDGEFARQTLAGVNPCSIQLVKSLPFES 402
               ET+                  RD++ W  D EFARQTLAG+NPCS++LV   P +S
Sbjct: 469 ----ETMN-----------------RDKFFWFRDEEFARQTLAGLNPCSLKLVTKWPLKS 528

Query: 403 ALDIHKYGPKESKFTPTWFEESMKGSITYIQAITQKKLFVLDYHDILMPYVIDVRNIDNK 462
            LD   YGP ES  T    E+ ++G  T  +AI +KKLF+LDYHD+ +PYV  VR I+  
Sbjct: 529 ELDPEIYGPPESAITKEIIEQEIRGFPTVQEAIREKKLFILDYHDLFLPYVSKVRKIEGT 588

Query: 463 TLYGSRTLFFLNSDDTLMPLGIELTRPPMDGDGQWKEIFKPSIKGTDLWQWRFAKAHVLS 522
           TLYGSRTLFFL  + TL PL IELTRPPMDG  QWK++F+PS   T +W WR AKAHVL+
Sbjct: 589 TLYGSRTLFFLTREGTLRPLAIELTRPPMDGKPQWKQVFQPSWNATGVWLWRLAKAHVLA 648

Query: 523 HDSCIHQLVIHWLRAHCCMETYAIATNRQLSTMHPIYRLLHPHFRYNMRINAQARKDLIS 582
           HDS  HQLV HWLR HC  E Y IAT+RQLS MHPIYR LHPHFRY M INA AR+ LI+
Sbjct: 649 HDSGYHQLVSHWLRTHCVTEPYIIATSRQLSVMHPIYRFLHPHFRYTMEINALARESLIN 708

Query: 583 AGGLIEGTFSTTSLSMELSSLVYKNEWQFDQQALPNDLIRRGMAKQKKDEEGHDVLELAI 642
           AGG+IE +FS    S+ELS++ Y  EW+FDQ+ALP DLIRRGMA +  D      L+L I
Sbjct: 709 AGGIIESSFSPGKYSLELSAVAYGKEWRFDQEALPADLIRRGMAVE--DPTAPHGLKLTI 768

Query: 643 KDYPFANDGLILWNAILDWVKDYVEHYYQGDKGNKLVKNDKELQAWWTEIKEKGHPDKKE 702
           +DYPFANDGL++W+AI  WV DYV HYY     + LV+ D ELQAWWTEIK  GH DKK+
Sbjct: 769 EDYPFANDGLLMWDAIKQWVTDYVNHYY---PDSSLVQTDGELQAWWTEIKTVGHADKKD 828

Query: 703 --GWPKLETKEDLIKIVSTIAWVGSGHHSTVNFIQYAYGGFIPNRPSIARINVPSEDSS- 762
              WP+L T EDLI I++T+ WV SGHH+ VNF QY Y G+ PNRP+IAR N+P+ED S 
Sbjct: 829 EPWWPELNTPEDLIGIITTMVWVTSGHHAAVNFGQYVYAGYFPNRPTIARTNMPTEDPSP 888

Query: 763 ---KKFINQPEEVLLKIFPSAAQGTAVATAMALLSTHSSDEQYIGEDIKIELAWDHDNKI 822
              K F+ +PE  LL+ FPS  Q T +   + +LS HS DE+YIGE  K+E AW  +  I
Sbjct: 889 EFWKSFLKKPEVALLRCFPSQIQATRIMAVLDILSNHSPDEEYIGE--KMEAAWAEEPVI 946

Query: 823 KVAFEKFCRKLKQLEGTIDDNNKDSEKKNRSGAGVIPYDVLKPYSKSGITGMGVPYSVS 857
           K AFE+F  +L  +EG IDD N +SE KNR+GAGV+PY++LKP+S+ G+TGMGVPYS+S
Sbjct: 949 KAAFERFKGRLLVIEGIIDDRNANSELKNRNGAGVVPYELLKPFSQPGVTGMGVPYSIS 946

BLAST of Cla003209 vs. TrEMBL
Match: A0A125S6K8_CUCME (Lipoxygenase OS=Cucumis melo var. makuwa GN=LOX13 PE=2 SV=1)

HSP 1 Score: 731.5 bits (1887), Expect = 1.2e-207
Identity = 423/873 (48.45%), Postives = 527/873 (60.37%), Query Frame = 1

Query: 9   VIVKTHKGETKKGD----SLPPEDTIFLTFVS---ETSHSCEKDNVVEGTVTIKKKKPGE 68
           VIVK   GET   +    S PP+  + L F S   +     EK  +   TV  K     E
Sbjct: 83  VIVKKITGETTSSNLMEASQPPQKLLQLGFASILLDPRTGSEKPPI---TVQAKLISEDE 142

Query: 69  DYNTYEGKIEVQDGFGRIGAVIVKISKDNSSTETFIDTVTIQKSMSDNLITFSCKSWVQP 128
               YE  +EV+  FG IGAVIV+    ++  E FI  V +   ++   +  SC SWVQP
Sbjct: 143 VEEIYEASLEVRSDFGEIGAVIVE---HHNEKEMFIKEVHLN-GLTSGPLNISCNSWVQP 202

Query: 129 KNDDDDDDEYSQRRIFFSTKSYLPDNTPAGLVKLRAEDLRNLRGEKPDGTIDMKERKENE 188
           K         +Q+R+FF+ KSYLP  TPAGL  +R  +L NLRG   +GT    ER+  +
Sbjct: 203 KTLVP-----TQKRVFFTNKSYLPSQTPAGLKSMRETELMNLRG---NGT---GERQSYD 262

Query: 189 RIYDYDVYNDLGETDDVPMSKRHVFGGSKEYPYPRRY----------DSFETRGSGKFYV 248
           RIYDYDVYNDLG+ D     KR + GG    PYPRR            + E R +   YV
Sbjct: 263 RIYDYDVYNDLGDPDKSEDLKRSILGGPDNSPYPRRCRTGRPATKTDPNSEQRATESIYV 322

Query: 249 PRDEEFSKVKQSYFPDMGQKNNMDELGEDHFTDLREIESLY--HGGIKTPTAPHKILHLE 308
           PRDE FS +K   F       N ++L     T +  ++  +  + G     A   +  ++
Sbjct: 323 PRDEAFSDIKSKAF-------NANQLSAALQTLIPRLQVHFDPNAGFPNFKAIDALFDVD 382

Query: 309 ISSILSPHQPPVLHSAISLTTILPPAESHISEYTFYINETLKSVKITVHLPHICLLIFPR 368
             ++  P       S  S   +LP     I +  +   E L   K  V +         R
Sbjct: 383 GFNLSPPDS-----SISSFKDLLP----WIFKLIYETGEFLFRFKPPVPM--------DR 442

Query: 369 DRYNWLSDGEFARQTLAGVNPCSIQLVKSLPFESALDIHKYGPKESKFTPTWFEESMKGS 428
           D++ WL D EFARQTLAG NPCSIQLVK  P  S LD   YGP ES F     ++ + GS
Sbjct: 443 DKFFWLRDEEFARQTLAGPNPCSIQLVKEWPLSSQLDPKVYGPPESAFNTQMIDQEI-GS 502

Query: 429 ITYIQAITQKKLFVLDYHDILMPYVIDVRNIDNKTLYGSRTLFFLNSDDTLMPLGIELTR 488
           +T  +AI +KKL++LDYHD+L+PYV  VR +   TLYGSRTLFFLN D+TL PL IELTR
Sbjct: 503 MTVHEAIQKKKLYMLDYHDMLLPYVQKVRALKGTTLYGSRTLFFLNEDETLRPLAIELTR 562

Query: 489 PPMDGDGQWKEIFKPSIKGTDLWQWRFAKAHVLSHDSCIHQLVIHWLRAHCCMETYAIAT 548
           PPM+G  QWK ++ PS K T LW WRFAKAHVL+HD+  HQLV HWLR HC +E Y IAT
Sbjct: 563 PPMNGKPQWKRVYGPSEKATSLWLWRFAKAHVLAHDAGYHQLVSHWLRTHCAVEPYVIAT 622

Query: 549 NRQLSTMHPIYRLLHPHFRYNMRINAQARKDLISAGGLIEGTFSTTSLSMELSSLVYKNE 608
           NRQLS MHPIYRLLHPHFRY M INA AR +LI+ GG IE TFS    SMELSS  Y  +
Sbjct: 623 NRQLSAMHPIYRLLHPHFRYTMEINASARTNLINGGGTIESTFSPLKYSMELSSTAYDLQ 682

Query: 609 WQFDQQALPNDLIRRGMAKQKKDEEGHDVLELAIKDYPFANDGLILWNAILDWVKDYVEH 668
           WQFD QALP DLI RG+A++  D      L+L IKDYPFANDGLILW+A+  WV +YV H
Sbjct: 683 WQFDLQALPADLIHRGLAEE--DPTASHGLKLHIKDYPFANDGLILWDALKQWVTEYVNH 742

Query: 669 YYQGDKGNKLVKNDKELQAWWTEIKEKGHPDKKE--GWPKLETKEDLIKIVSTIAWVGSG 728
           YY       LV  D ELQAWWTEI+  GH DK+E   WP L T +DLI IV+ IAWV S 
Sbjct: 743 YYPDP---YLVTTDTELQAWWTEIRTVGHADKQEKPWWPLLNTPQDLIDIVTNIAWVASA 802

Query: 729 HHSTVNFIQYAYGGFIPNRPSIARINVPSEDSS----KKFINQPEEVLLKIFPSAAQGTA 788
           HH+ VNF QYAY G+ PNRPSI R N+P+ED++    K F+ +PEE+LL  FPS  Q T 
Sbjct: 803 HHAAVNFGQYAYSGYFPNRPSITRTNMPTEDNNPALWKSFLEKPEELLLDSFPSQFQATQ 862

Query: 789 VATAMALLSTHSSDEQYIGEDIKIELAWDHDNKIKVAFEKFCRKLKQLEGTIDDNNKDSE 848
           V   M +LS+HS DE+YIG+D  +E +W  +  IK AFE+F   +K +E  ID  N DS 
Sbjct: 863 VMLVMNMLSSHSPDEEYIGKD--MEPSWGDNPTIKAAFERFSTSIKNMELIIDHRNSDSN 905

Query: 849 KKNRSGAGVIPYDVLKPYSKSGITGMGVPYSVS 857
            KNR+GAGV PY++LKP+S+SG+TG GVPYS+S
Sbjct: 923 LKNRTGAGVTPYELLKPFSESGVTGKGVPYSIS 905

BLAST of Cla003209 vs. TrEMBL
Match: S4UKZ5_MALDO (Lipoxygenase OS=Malus domestica GN=LOX6b PE=2 SV=1)

HSP 1 Score: 729.6 bits (1882), Expect = 4.5e-207
Identity = 405/843 (48.04%), Postives = 516/843 (61.21%), Query Frame = 1

Query: 43  EKDNVVEGTVTIKKKKPGEDYNTYEGKIEVQDGFGRIGAVIVKISKDNSSTETFIDTVTI 102
           E+   V G     +++ GE    YE   +V   FG IGA++V+   +    E F+  + +
Sbjct: 158 EEKEKVAGYAHRSRRQEGEII--YETDFKVPVDFGEIGAILVE---NEHRKEMFLKEIVV 217

Query: 103 QKSMSDNLITFSCKSWVQPKNDDDDDDEYSQRRIFFSTKSYLPDNTPAGLVKLRAEDLRN 162
              +    +  SC SW+  K D+        +R+FF+ KSYLP  TP+GL KLR E+L  
Sbjct: 218 D-GLPCGSVHHSCNSWIHSKYDNP------AKRVFFTNKSYLPSQTPSGLAKLREEELVT 277

Query: 163 LRGEKPDGTIDMKERKENERIYDYDVYNDLGETDDVPMSKRHVFGGSKEYPYPRRYDS-- 222
           LRG          ERK  ERIYDYDVYNDLG+ D     +R V GG KE+PYPRR  +  
Sbjct: 278 LRGN------GQGERKSFERIYDYDVYNDLGDPDKNLRLERPVLGG-KEFPYPRRCRTGR 337

Query: 223 --------FETRGSGKFYVPRDEEFSKVKQSYF-------------PDMGQKNNMDELGE 282
                   +E R    +YVPRDE FS+VKQ  F             P +       +LG 
Sbjct: 338 LPCDTGSLYEKRSRKHWYVPRDEAFSEVKQLTFSAKTLYSVMHALVPSLEIAIADTDLGF 397

Query: 283 DHFTDLREIESLYHGGIKTPTAPHKILHLEISSILSPHQPPVLHSAISLTTILPPAESHI 342
            +FT    I+SL+H GI+ P  P K     +   L P    V+ S   +   +PP     
Sbjct: 398 KYFT---AIDSLFHEGIQLP--PFK--EQGVLKALLPRLVKVMSSGDDVLRFVPP----- 457

Query: 343 SEYTFYINETLKSVKITVHLPHICLLIFPRDRYNWLSDGEFARQTLAGVNPCSIQLVKSL 402
                   ET+                  RD++ W  D EFARQTLAG+NP SI+LV   
Sbjct: 458 --------ETMN-----------------RDKFFWFRDDEFARQTLAGLNPYSIKLVTEW 517

Query: 403 PFESALDIHKYGPKESKFTPTWFEESMKGSITYIQAITQKKLFVLDYHDILMPYVIDVRN 462
           P +S LD   YGP ES  T    E+ ++G  T  +AI +KKLF+LDYHD+ +PYV  VR 
Sbjct: 518 PLKSELDPEIYGPPESAITKEIIEQEIRGFATITEAIEEKKLFILDYHDLFLPYVSKVRK 577

Query: 463 IDNKTLYGSRTLFFLNSDDTLMPLGIELTRPPMDGDGQWKEIFKPSIKGTDLWQWRFAKA 522
           ++  TLYGSRTLFFL  + TL PL IELTRPPMDG  QWK++F+P+   TD+W WR AKA
Sbjct: 578 LEGTTLYGSRTLFFLTPEGTLRPLVIELTRPPMDGKPQWKQVFQPAWNATDVWLWRLAKA 637

Query: 523 HVLSHDSCIHQLVIHWLRAHCCMETYAIATNRQLSTMHPIYRLLHPHFRYNMRINAQARK 582
           HVL+HDS  HQLV HWLR HC  E Y IATNRQLS MHPIYRLLHPHFRY M IN+ AR+
Sbjct: 638 HVLAHDSGYHQLVSHWLRTHCATEPYIIATNRQLSVMHPIYRLLHPHFRYTMEINSLARE 697

Query: 583 DLISAGGLIEGTFSTTSLSMELSSLVYKNEWQFDQQALPNDLIRRGMAKQKKDEEGHDVL 642
            LI+AGG+IE +FS    S+EL S+VY  EW+FDQ+ALP DLIRRGMA +  D      L
Sbjct: 698 SLINAGGIIETSFSPKKYSLELCSVVYGKEWRFDQEALPADLIRRGMAVE--DPTAPHGL 757

Query: 643 ELAIKDYPFANDGLILWNAILDWVKDYVEHYYQGDKGNKLVKNDKELQAWWTEIKEKGHP 702
            L I+DYPFANDGL+LW+AI  WV DYV HYY     + +V+ D+ELQAWWTEIK  GH 
Sbjct: 758 RLTIEDYPFANDGLLLWDAIKQWVTDYVNHYY---PDSSIVQTDQELQAWWTEIKTVGHA 817

Query: 703 DKKE--GWPKLETKEDLIKIVSTIAWVGSGHHSTVNFIQYAYGGFIPNRPSIARINVPSE 762
           DKK+   WP+L T EDL+ I++T+ WV SGHH+ VNF QYAY G+ P+RP+IAR N+P+E
Sbjct: 818 DKKDEPWWPELNTPEDLMGIITTMVWVASGHHAAVNFGQYAYAGYFPSRPTIARTNMPTE 877

Query: 763 DSS----KKFINQPEEVLLKIFPSAAQGTAVATAMALLSTHSSDEQYIGEDIKIELAWDH 822
           D S    K F+ +P+  LL+ FP+  Q T +   + +LS HS DE+YIGE  K+E AW  
Sbjct: 878 DPSEEDWKNFVRKPDSALLQCFPTQIQATTIMAVLDILSNHSPDEEYIGE--KMEQAWAE 937

Query: 823 DNKIKVAFEKFCRKLKQLEGTIDDNNKDSEKKNRSGAGVIPYDVLKPYSKSGITGMGVPY 857
           +  IK AFE+F  +L  LE TIDD N  SE KNR+GAGV+PY++LKP+S+ G+TG GVPY
Sbjct: 938 EPVIKAAFERFKGRLLALERTIDDRNASSELKNRNGAGVVPYELLKPFSQPGVTGKGVPY 937

BLAST of Cla003209 vs. TrEMBL
Match: S4UKU6_MALDO (Lipoxygenase OS=Malus domestica GN=LOX6b PE=2 SV=1)

HSP 1 Score: 728.0 bits (1878), Expect = 1.3e-206
Identity = 404/843 (47.92%), Postives = 515/843 (61.09%), Query Frame = 1

Query: 43  EKDNVVEGTVTIKKKKPGEDYNTYEGKIEVQDGFGRIGAVIVKISKDNSSTETFIDTVTI 102
           E+   V G     +++ GE    YE   +V   FG IGA++V+   +    E F+  + +
Sbjct: 158 EEKEKVAGYAHRSRRQEGEII--YETDFKVPVDFGEIGAILVE---NEHRKEMFLKEIVV 217

Query: 103 QKSMSDNLITFSCKSWVQPKNDDDDDDEYSQRRIFFSTKSYLPDNTPAGLVKLRAEDLRN 162
              +    +  SC SW+  K D+        +R+FF+ KSYLP  TP+GL KLR E+L  
Sbjct: 218 D-GLPCGSVHHSCNSWIHSKYDNP------AKRVFFTNKSYLPSQTPSGLAKLREEELVT 277

Query: 163 LRGEKPDGTIDMKERKENERIYDYDVYNDLGETDDVPMSKRHVFGGSKEYPYPRRYDS-- 222
           LRG          ERK  ERIYDYDVYNDLG+ D     +R V GG KE+PYPRR  +  
Sbjct: 278 LRGN------GQGERKSFERIYDYDVYNDLGDPDKNLRLERPVLGG-KEFPYPRRCRTGR 337

Query: 223 --------FETRGSGKFYVPRDEEFSKVKQSYF-------------PDMGQKNNMDELGE 282
                   +E R    +YVPRDE FS+VKQ  F             P +       +LG 
Sbjct: 338 LPCDTGSLYEKRSRKHWYVPRDEAFSEVKQLTFSAKTLYSVMHALVPSLEIAIADTDLGF 397

Query: 283 DHFTDLREIESLYHGGIKTPTAPHKILHLEISSILSPHQPPVLHSAISLTTILPPAESHI 342
            +FT    I+SL+H GI+ P  P K     +   L P    V+ S   +   +PP     
Sbjct: 398 KYFT---AIDSLFHEGIQLP--PFK--EQGVLKALLPRLVKVMSSGDDVLRFVPP----- 457

Query: 343 SEYTFYINETLKSVKITVHLPHICLLIFPRDRYNWLSDGEFARQTLAGVNPCSIQLVKSL 402
                   ET+                  RD++ W  D EFARQTLAG+NP SI+LV   
Sbjct: 458 --------ETMN-----------------RDKFFWFRDDEFARQTLAGLNPYSIKLVTEW 517

Query: 403 PFESALDIHKYGPKESKFTPTWFEESMKGSITYIQAITQKKLFVLDYHDILMPYVIDVRN 462
           P +S LD   YGP ES  T    E+ ++G  T  +AI +KKLF+LDYHD+ +PYV  VR 
Sbjct: 518 PLKSELDPEIYGPPESAITKEIIEQEIRGFATITEAIEEKKLFILDYHDLFLPYVSKVRK 577

Query: 463 IDNKTLYGSRTLFFLNSDDTLMPLGIELTRPPMDGDGQWKEIFKPSIKGTDLWQWRFAKA 522
           ++  TLYGSRTLFFL  + TL PL IELTRPPMDG  QWK++F+P+   TD+W WR AKA
Sbjct: 578 LEGTTLYGSRTLFFLTPEGTLRPLVIELTRPPMDGKPQWKQVFQPAWNATDVWLWRLAKA 637

Query: 523 HVLSHDSCIHQLVIHWLRAHCCMETYAIATNRQLSTMHPIYRLLHPHFRYNMRINAQARK 582
           HVL+HDS  HQLV HWLR HC  E Y IATNRQLS MHPIYRLLHPHFRY M IN+ AR+
Sbjct: 638 HVLAHDSGYHQLVSHWLRTHCATEPYIIATNRQLSVMHPIYRLLHPHFRYTMEINSLARE 697

Query: 583 DLISAGGLIEGTFSTTSLSMELSSLVYKNEWQFDQQALPNDLIRRGMAKQKKDEEGHDVL 642
            LI+AGG+IE +FS    S+EL S+ Y  EW+FDQ+ALP DLIRRGMA +  D      L
Sbjct: 698 SLINAGGIIETSFSPKKYSLELCSVAYGKEWRFDQEALPADLIRRGMAVE--DPTAPHGL 757

Query: 643 ELAIKDYPFANDGLILWNAILDWVKDYVEHYYQGDKGNKLVKNDKELQAWWTEIKEKGHP 702
            L I+DYPFANDGL+LW+AI  WV DYV HYY     + +V+ D+ELQAWWTEIK  GH 
Sbjct: 758 RLTIEDYPFANDGLLLWDAIKQWVTDYVNHYY---PDSSIVQTDQELQAWWTEIKTVGHA 817

Query: 703 DKKE--GWPKLETKEDLIKIVSTIAWVGSGHHSTVNFIQYAYGGFIPNRPSIARINVPSE 762
           DKK+   WP+L T EDL+ I++T+ WV SGHH+ VNF QYAY G+ P+RP+IAR N+P+E
Sbjct: 818 DKKDEPWWPELNTPEDLMGIITTMVWVASGHHAAVNFGQYAYAGYFPSRPTIARTNMPTE 877

Query: 763 DSS----KKFINQPEEVLLKIFPSAAQGTAVATAMALLSTHSSDEQYIGEDIKIELAWDH 822
           D S    K F+ +P+  LL+ FP+  Q T +   + +LS HS DE+YIGE  K+E AW  
Sbjct: 878 DPSEEDWKNFVRKPDSALLQCFPTQIQATTIMAVLDILSNHSPDEEYIGE--KMEQAWAE 937

Query: 823 DNKIKVAFEKFCRKLKQLEGTIDDNNKDSEKKNRSGAGVIPYDVLKPYSKSGITGMGVPY 857
           +  IK AFE+F  +L  LE TIDD N  SE KNR+GAGV+PY++LKP+S+ G+TG GVPY
Sbjct: 938 EPVIKAAFERFKGRLLALERTIDDRNASSELKNRNGAGVVPYELLKPFSQPGVTGKGVPY 937

BLAST of Cla003209 vs. TrEMBL
Match: F6GUA6_VITVI (Lipoxygenase OS=Vitis vinifera GN=VIT_06s0004g01480 PE=3 SV=1)

HSP 1 Score: 726.9 bits (1875), Expect = 2.9e-206
Identity = 400/819 (48.84%), Postives = 505/819 (61.66%), Query Frame = 1

Query: 66  YEGKIEVQDGFGRIGAVIVKISKDNSSTETFIDTVTIQKSMSDNLITFSCKSWVQPKNDD 125
           YE +  +   FG IGAV+V+   +   +E F+  + +   + +  I F+C SWVQ K DD
Sbjct: 33  YESEFVIPGDFGEIGAVLVQ---NEYRSEMFLKYIVLN-GLPNGPIAFNCGSWVQSKFDD 92

Query: 126 DDDDEYSQRRIFFSTKSYLPDNTPAGLVKLRAEDLRNLRGEKPDGTIDMKERKENERIYD 185
            +      +RIFFS KSYLP  TP GL  LR ++L NLRG          ERK ++RIYD
Sbjct: 93  PE------KRIFFSNKSYLPSQTPRGLKDLREKELANLRGNGEG------ERKTSDRIYD 152

Query: 186 YDVYNDLGETDDVPMSKRHVFGGSKEYPYPRRY---------DSFETRGSGKFYVPRDEE 245
           YDVYNDLG  D      R V GG++ YPYPRR          D      SG FYVPRDEE
Sbjct: 153 YDVYNDLGNPDSKSELGRSVLGGNENYPYPRRCRTGRAPCQTDPLSETRSGSFYVPRDEE 212

Query: 246 FSKVKQSYF-------------PDMGQKNNMDELGEDHFTDLREIESLYHGGIKTPTAPH 305
           FS+VK++ F             P +        LG   F+D   I+ LY+ GI  P   +
Sbjct: 213 FSEVKEASFLTKTVDSVLHALKPSLETSLLDSNLGFPLFSD---IDQLYYQGITIPKLKN 272

Query: 306 KILHLEISSILSPHQPPVLHSAISLTTILPPAESHISEYTFYINETLKSVKITVHLPHIC 365
             L                     L  ILP     +S+         K   +    P   
Sbjct: 273 PGL---------------------LQRILPRLVKAVSD--------AKDRLLKFETP--- 332

Query: 366 LLIFPRDRYNWLSDGEFARQTLAGVNPCSIQLVKSLPFESALDIHKYGPKESKFTPTWFE 425
             +F +D+++WL D EF+RQTLAGVNP SI+LV   P +SALD   YGP ES  T    E
Sbjct: 333 -AMFLKDKFSWLRDEEFSRQTLAGVNPYSIKLVMEWPLKSALDPDVYGPPESAITKELVE 392

Query: 426 ESMKGSITYIQAITQKKLFVLDYHDILMPYVIDVRNIDNKTLYGSRTLFFLNSDDTLMPL 485
             +KG +T  +A+ QKKLF++DYHD+L+PYV  VR I+  TLYGSR LFFL  D TL PL
Sbjct: 393 RGIKGFMTVDEALEQKKLFIIDYHDLLLPYVSKVRQIEGTTLYGSRALFFLTPDCTLKPL 452

Query: 486 GIELTRPPMDGDGQWKEIFKPSIKGTDLWQWRFAKAHVLSHDSCIHQLVIHWLRAHCCME 545
            IELTRPPMDG  QWK++F P ++ TD W W+FAK H L+HDS  H+LV HWLR HC  E
Sbjct: 453 AIELTRPPMDGKPQWKQVFTPGLEATDHWLWKFAKTHFLAHDSGYHELVSHWLRTHCATE 512

Query: 546 TYAIATNRQLSTMHPIYRLLHPHFRYNMRINAQARKDLISAGGLIEGTFSTTSLSMELSS 605
            Y IATNRQLS MHPIY+LLHPH RY M+INA AR+ LI+A G+IE +FST   SMELSS
Sbjct: 513 PYVIATNRQLSVMHPIYKLLHPHLRYTMQINALAREVLINADGIIETSFSTRKYSMELSS 572

Query: 606 LVYKNEWQFDQQALPNDLIRRGMAKQKKDEEGHDVLELAIKDYPFANDGLILWNAILDWV 665
             Y  +W+FD++ALP DLI RG+A +  D      L+L I+DYPFANDGLILW+A+  WV
Sbjct: 573 AAYDQQWRFDREALPADLINRGIAVE--DPSASHGLKLLIEDYPFANDGLILWDALKQWV 632

Query: 666 KDYVEHYYQGDKGNKLVKNDKELQAWWTEIKEKGHPDKKE--GWPKLETKEDLIKIVSTI 725
            DYV +YY   K   +V++D ELQAWWTEI+ KGH DKK+   WP L+T EDLI I++TI
Sbjct: 633 ADYVNYYY---KDASMVQSDPELQAWWTEIRTKGHEDKKDEPWWPVLQTPEDLIGIIATI 692

Query: 726 AWVGSGHHSTVNFIQYAYGGFIPNRPSIARINVPSEDSS----KKFINQPEEVLLKIFPS 785
           AWV S HHS VNF QYA+  + PNRP+IAR N+PSED +    K+F++ P   LL  FPS
Sbjct: 693 AWVASAHHSAVNFGQYAFAAYFPNRPTIARTNMPSEDPTREGWKRFLDNPHFELLVCFPS 752

Query: 786 AAQGTAVATAMALLSTHSSDEQYIGEDIKIELAWDHDNKIKVAFEKFCRKLKQLEGTIDD 845
             Q T V   + +LS HS DE+YIGE   +E AW  +  IK AFE+F  +LK+LE  ID 
Sbjct: 753 QVQATKVIAILDVLSNHSPDEEYIGE--YMEPAWGEEPDIKEAFERFSARLKELEVIIDA 792

Query: 846 NNKDSEKKNRSGAGVIPYDVLKPYSKSGITGMGVPYSVS 857
            N D+  KNR GAGV+PY++LKP+S++G+TG GVPYS+S
Sbjct: 813 RNADNSLKNRGGAGVVPYELLKPFSEAGVTGKGVPYSIS 792

BLAST of Cla003209 vs. NCBI nr
Match: gi|659097677|ref|XP_008449754.1| (PREDICTED: lipoxygenase 2, chloroplastic-like [Cucumis melo])

HSP 1 Score: 958.0 bits (2475), Expect = 1.1e-275
Identity = 512/875 (58.51%), Postives = 608/875 (69.49%), Query Frame = 1

Query: 1   MECTNTATVIVKTHKGETKKGDSLPPEDTIFLTFVS-ETSHSCEKDNVVEGTVT-IKKKK 60
           +E T + TV  KT  G T       P   I L F S E     ++   ++  V  I+   
Sbjct: 3   VEATVSVTVKPKT-TGPTY------PYANIILKFASIELDSDQQQKQFIKCVVEPIRPDD 62

Query: 61  PGEDYNTYEGKIEVQDGFGRIGAVIVKISKDNSSTETFIDTVTIQKSMSDNLITFSCKSW 120
             E+Y  YEG++EV +G+G IGAVIV++  D +  E FIDT+++    S +  TFSCKSW
Sbjct: 63  DTEEYKEYEGEVEVPEGYGEIGAVIVELECDTAKYEKFIDTISLTDKKSRHSTTFSCKSW 122

Query: 121 VQPKNDDDDDDEYSQRRIFFSTKSYLPDNTPAGLVKLRAEDLRNLRGEKPDGTIDMKERK 180
           VQ K+  D      QRRIFFSTKSYLP  TP GL+KLRAEDL NLRG KPDGT+DM ERK
Sbjct: 123 VQSKSVLD------QRRIFFSTKSYLPGKTPGGLLKLRAEDLSNLRGIKPDGTVDMNERK 182

Query: 181 ENERIYDYDVYNDLGETDDVPMSKRHVFGGSKEYPYPRRYDS----------FETRGSGK 240
             ERIYDYD YNDLG+ D     KR V GGS E PYPRR  +           E R   +
Sbjct: 183 AFERIYDYDFYNDLGDCDGPAEWKRPVLGGS-ERPYPRRCRTGRLHCIADPVSEKRSKER 242

Query: 241 FYVPRDEEFSKVKQSYFPDMGQKNNMDELGEDHFTDLREIESLYHGGIKTPTAPHKILHL 300
           FYVPRDEEFS+VKQ YFP   + +N D LG++ F DL  IES++  GIK P APHK+L  
Sbjct: 243 FYVPRDEEFSEVKQHYFPS-SEPDNKDLLGKNSFHDLPHIESMFREGIKAPDAPHKLLKF 302

Query: 301 EISSILSPHQPPVLHSAISLTTIL---PPAESHISEYTFYINETLKSVKITVHLPHICLL 360
            +S+I++P   P LHS  S+ + L   PP ES                            
Sbjct: 303 NLSTIVTPDHKPDLHSNSSVPSSLLHYPPPES---------------------------- 362

Query: 361 IFPRDRYNWLSDGEFARQTLAGVNPCSIQLVKSLPFESALDIHKYGPKESKFTPTWFEES 420
            + RDRY+WLSD EFARQTLAG+NP SIQLV  LP  S LD   YGP+ES F  T  +E 
Sbjct: 363 -YRRDRYSWLSDTEFARQTLAGLNPYSIQLVTRLPLMSELDPEIYGPQESAFNHTKVQEL 422

Query: 421 MKGSITYIQAITQKKLFVLDYHDILMPYVIDVRNIDNKTLYGSRTLFFLNSDDTLMPLGI 480
           +       +AI  K+LFV+DYHD LMPYV  VR I+  TLYGSRTLFFL SD TL+PLGI
Sbjct: 423 LGCFNEVNEAIECKRLFVVDYHDTLMPYVNKVRTINGTTLYGSRTLFFLKSDGTLIPLGI 482

Query: 481 ELTRPPMDGDGQWKEIFKPSIKGTDLWQWRFAKAHVLSHDSCIHQLVIHWLRAHCCMETY 540
           ELTRPP+DG  QWK+IF P  + TD+W WR AKAHVL+HDSCIHQLVIHWLRAHCCME Y
Sbjct: 483 ELTRPPVDGYPQWKQIFTPGTEATDVWLWRIAKAHVLAHDSCIHQLVIHWLRAHCCMEPY 542

Query: 541 AIATNRQLSTMHPIYRLLHPHFRYNMRINAQARKDLISAGGLIEGTFSTTSLSMELSSLV 600
           AIATNRQLST+HPIYRLLHPHFRYNMRINA AR+ LI+AGG+IEGTFST S SMELSS V
Sbjct: 543 AIATNRQLSTLHPIYRLLHPHFRYNMRINANARESLINAGGIIEGTFSTASYSMELSSSV 602

Query: 601 YKNEWQFDQQALPNDLIRRGMAKQKKDEEGHDVLELAIKDYPFANDGLILWNAILDWVKD 660
           Y+++W+FD+QA P DLIRRGMA++KKDE G D+LEL IKDYPFANDGLILWNA+L+WV +
Sbjct: 603 YRDQWRFDEQAFPEDLIRRGMAERKKDEYGRDILELTIKDYPFANDGLILWNALLEWVTE 662

Query: 661 YVEHYYQGDKGNKLVKNDKELQAWWTEIKEKGHPDKKEGWPKLETKEDLIKIVSTIAWVG 720
           YV HYY GD  N ++ NDKELQAWW EI+EKGHPDKKEGWP L+T+ DLIKI STIAWVG
Sbjct: 663 YVNHYY-GDDENAVI-NDKELQAWWNEIQEKGHPDKKEGWPTLKTRNDLIKIASTIAWVG 722

Query: 721 SGHHSTVNFIQYAYGGFIPNRPSIARINVPSED---SSKKFINQPEEVLLKIFPSAAQGT 780
           SGHH++VNFIQYAY G+ PNRPSIAR N+ +ED     ++FI+ PE VLL++FPS  Q T
Sbjct: 723 SGHHASVNFIQYAYAGYTPNRPSIARTNILTEDYHQLPEEFIDLPENVLLQVFPSVDQAT 782

Query: 781 AVATAMALLSTHSSDEQYIGEDIKIELAWDHDNKIKVAFEKFCRKLKQLEGTIDDNNKDS 840
            V T M LLS HS DE+YIG  +++E AW  +  I  AF++F   LK LE  ID+NNK+ 
Sbjct: 783 MVTTTMILLSAHSPDEEYIG--VEVEPAWALEPAIDKAFKRFQANLKDLEQQIDENNKNK 829

Query: 841 EKKNRSGAGVIPYDVLKPYSKSGITGMGVPYSVST 858
             KNR GAGV+PY+VLKP S  GITG GVPYSVST
Sbjct: 843 SLKNRHGAGVVPYEVLKPTSTDGITGRGVPYSVST 829

BLAST of Cla003209 vs. NCBI nr
Match: gi|449448968|ref|XP_004142237.1| (PREDICTED: lipoxygenase 2, chloroplastic-like [Cucumis sativus])

HSP 1 Score: 952.2 bits (2460), Expect = 6.2e-274
Identity = 495/814 (60.81%), Postives = 591/814 (72.60%), Query Frame = 1

Query: 61  EDYNTYEGKIEVQDGFGRIGAVIVKISKDNSSTETFIDTVTIQKSMSDNLITFSCKSWVQ 120
           E+Y  Y G++EV +G+G IGAVIV++  D +  E FIDT++I    S N  TFSCKSWVQ
Sbjct: 58  EEYKKYVGEVEVPEGYGEIGAVIVELEGDTTKYEKFIDTISITDKKSRNSTTFSCKSWVQ 117

Query: 121 PKNDDDDDDEYSQRRIFFSTKSYLPDNTPAGLVKLRAEDLRNLRGEKPDGTIDMKERKEN 180
            K+  D      QRR+FFSTKSYLP+ TP GL+KLRAEDL NLRG K D T+DM ERK  
Sbjct: 118 SKSVLD------QRRVFFSTKSYLPERTPGGLLKLRAEDLSNLRGIKADETVDMNERKAF 177

Query: 181 ERIYDYDVYNDLGETDDVPMSKRHVFGGSKEYPYPRRYDS----------FETRGSGKFY 240
           ERIYDYD YNDLG+ D     KR V GGS ++PYPRR  +           E R   +FY
Sbjct: 178 ERIYDYDFYNDLGDCDGPAEWKRPVLGGS-DHPYPRRCRTGRLHCKTDPVSEKRSKERFY 237

Query: 241 VPRDEEFSKVKQSYFPDMGQKNNMDELGEDHFTDLREIESLYHGGIKTPTAPHKILHLEI 300
           VPRDEEFS+VKQ YFP   + +N D LG+D F+DL  IES++  GIK P APHK+L   +
Sbjct: 238 VPRDEEFSEVKQHYFPS-SEPDNKDLLGKDSFSDLPHIESMFREGIKAPHAPHKLLTFNL 297

Query: 301 SSILSPHQPPV-LHSAISLTTIL---PPAESHISEYTFYINETLKSVKITVHLPHICLLI 360
           S+I++P +P +  +S  S+ + L   PP ES                             
Sbjct: 298 STIVTPDKPDLHSNSNSSVPSSLLHYPPPES----------------------------- 357

Query: 361 FPRDRYNWLSDGEFARQTLAGVNPCSIQLVKSLPFESALDIHKYGPKESKFTPTWFEESM 420
           + RDRY+WLSD EFARQTLAG+NP SIQLV  LP  S LD   YGP+ES F  T  +E +
Sbjct: 358 YRRDRYSWLSDTEFARQTLAGLNPYSIQLVTRLPLMSELDPDTYGPQESAFNHTKVQELI 417

Query: 421 KGSITYIQAITQKKLFVLDYHDILMPYVIDVRNIDNKTLYGSRTLFFLNSDDTLMPLGIE 480
             SI   +AI  K+LFVLDYHD LMPYV  VR I+  TLYGSRTLFFL SDDTL+PLGIE
Sbjct: 418 GCSIEVNEAIELKRLFVLDYHDTLMPYVNKVRKINGTTLYGSRTLFFLKSDDTLIPLGIE 477

Query: 481 LTRPPMDGDGQWKEIFKPSIKGTDLWQWRFAKAHVLSHDSCIHQLVIHWLRAHCCMETYA 540
           LTRPP+DG  QWK+IF P I+ TDLW WR AKAHVL+HDSCIHQLVIHWLRAHCCME YA
Sbjct: 478 LTRPPVDGYPQWKQIFTPGIEATDLWLWRIAKAHVLAHDSCIHQLVIHWLRAHCCMEPYA 537

Query: 541 IATNRQLSTMHPIYRLLHPHFRYNMRINAQARKDLISAGGLIEGTFSTTSLSMELSSLVY 600
           IATNRQLST+HPIYRLLHPHFRYNMRINA AR+ LI+AGG+IE TFST S S+ELSS +Y
Sbjct: 538 IATNRQLSTVHPIYRLLHPHFRYNMRINANARESLINAGGIIESTFSTASYSVELSSSMY 597

Query: 601 KNEWQFDQQALPNDLIRRGMAKQKKDEEGHDVLELAIKDYPFANDGLILWNAILDWVKDY 660
           +++W+FD+QALP DLIRRGMA++KK + G DVLELAIKDYPFANDGL+LWNA+L+WV +Y
Sbjct: 598 RDQWRFDEQALPEDLIRRGMAERKKGDYGRDVLELAIKDYPFANDGLLLWNALLEWVTEY 657

Query: 661 VEHYYQGDKGNKLVKNDKELQAWWTEIKEKGHPDKKEGWPKLETKEDLIKIVSTIAWVGS 720
           V HYY GD  N ++ NDKELQAWW EI+EKGHPDKKEGWP L+T+ DLIKI STIAWVGS
Sbjct: 658 VNHYY-GDDENAVI-NDKELQAWWNEIQEKGHPDKKEGWPTLKTRNDLIKIASTIAWVGS 717

Query: 721 GHHSTVNFIQYAYGGFIPNRPSIARINVPSED---SSKKFINQPEEVLLKIFPSAAQGTA 780
           GHH++VNFIQYAY G+ PNRPSIAR N+ +ED     ++FI+ PE  LL++FPS  Q + 
Sbjct: 718 GHHASVNFIQYAYAGYTPNRPSIARTNILTEDYHQLPEEFIDLPENELLQVFPSVDQASV 777

Query: 781 VATAMALLSTHSSDEQYIGEDIKIELAWDHDNKIKVAFEKFCRKLKQLEGTIDDNNKDSE 840
           V T M LLS HS DE+YIG++  IE AW  +  I  AF++F   LK LE  ID+NNK+++
Sbjct: 778 VTTTMILLSAHSPDEEYIGDE--IEPAWALEPSISKAFKRFQASLKDLEQQIDENNKNNK 830

Query: 841 KKNRSGAGVIPYDVLKPYSKSGITGMGVPYSVST 858
            KNR GAGV+PYDVLKP S  GITG GVPYSVST
Sbjct: 838 LKNRHGAGVVPYDVLKPTSTYGITGRGVPYSVST 830

BLAST of Cla003209 vs. NCBI nr
Match: gi|778693086|ref|XP_004142240.2| (PREDICTED: linoleate 13S-lipoxygenase 2-1, chloroplastic-like [Cucumis sativus])

HSP 1 Score: 910.6 bits (2352), Expect = 2.1e-261
Identity = 486/859 (56.58%), Postives = 580/859 (67.52%), Query Frame = 1

Query: 19  KKGDSLPPEDTIFLTFVS-ETSHSCEKDNVVEGTVTIKKKKPGEDYNTYEGKIEVQDGFG 78
           K  ++ P +DT+ L F S +     ++  ++ G   + K +   +   Y  KI+V  GFG
Sbjct: 14  KINETFPSDDTLLLFFASSDLDRDGQQKPLIGGVAELIKNESTAEKYKYVAKIQVPKGFG 73

Query: 79  RIGAVIVKISKDNSSTETFIDTVTIQKSMSDNLITFSCKSWVQPKNDDDDDDEYSQRRIF 138
            IGAVIV++  D+S  E FIDTV +    S N ITFSC SWVQPK+   D     QRR+F
Sbjct: 74  EIGAVIVELKDDSS--EKFIDTVIVANPTSHNTITFSCTSWVQPKSLIPD-----QRRVF 133

Query: 139 FSTKSYLPDNTPAGLVKLRAEDLRNLRGEKPDGTIDMKERKENERIYDYDVYNDLGETDD 198
           FSTKSYLP +TPAGL+KLRAEDL NLRG+K DGT+D  ERK  ERIYDYDVYNDLG+ D 
Sbjct: 134 FSTKSYLPGDTPAGLLKLRAEDLENLRGQKEDGTVDTNERKAFERIYDYDVYNDLGDPDV 193

Query: 199 VPMSKRHVFGGSKEYPYPRRY----------DSFETRGSGKFYVPRDEEFSKVKQSYFPD 258
               KR V GGS +YPYPRR              E R +  FYVPRDE FS+VKQ  FP 
Sbjct: 194 DTKWKRPVLGGSVQYPYPRRCRTGRPATVSDPKSEQRSTESFYVPRDEAFSEVKQKMFPS 253

Query: 259 MGQKNNMDELGEDHFTDLREIESLYHGGIKTPTAPHKILHLEISSILS----PHQPPVLH 318
              K   D+LG   FTD  +I+ ++  GI  P+ PH+IL   I S L+    P  PPV  
Sbjct: 254 NPGKK--DKLGAKPFTDFTQIDLMFRDGIAPPSNPHEILQFNIISTLNASNQPAPPPVQP 313

Query: 319 SAISLTTILPPAESHISEYTFYINETLKSVKITVHLPHICLLIFPRDRYNWLSDGEFARQ 378
                     P + +     F   E LK                 RD++NWLSD EFARQ
Sbjct: 314 I---------PVQPNTPVIKFPPPEALK-----------------RDKFNWLSDSEFARQ 373

Query: 379 TLAGVNPCSIQLVKSLPFESALDIHKYGPKESKFTPTWFEESMKGSITYIQAITQKKLFV 438
           T+AG+NP  IQLVKS P +S LD  +YGP+ES FT    ++ +  SIT  +AI QKKLFV
Sbjct: 374 TIAGLNPYCIQLVKSWPLKSELDPVEYGPQESAFTTELVQKLIGSSITVEEAIAQKKLFV 433

Query: 439 LDYHDILMPYVIDVRNIDNKTLYGSRTLFFLNSDDTLMPLGIELTRPPMDGDGQWKEIFK 498
           LDYHD LM YV  VR+I   TLYGSRTLFFLN DDTL PLGIELTRPPM G  QWK++F 
Sbjct: 434 LDYHDTLMQYVEKVRSIKRTTLYGSRTLFFLNDDDTLSPLGIELTRPPMAGKPQWKQVFS 493

Query: 499 PSIKGTDLWQWRFAKAHVLSHDSCIHQLVIHWLRAHCCMETYAIATNRQLSTMHPIYRLL 558
           PS + T+ W WR AKAHVL+HDSC+HQLVIHWLR HCCME YAIA NRQLSTMHPIYRLL
Sbjct: 494 PSTQATNAWLWRLAKAHVLAHDSCVHQLVIHWLRTHCCMEPYAIALNRQLSTMHPIYRLL 553

Query: 559 HPHFRYNMRINAQARKDLISAGGLIEGTFSTTSLSMELSSLVYKNEWQFDQQALPNDLIR 618
           HPHFR+NMRIN+ AR+ LI+AGG+IE TFS    SME SSLVYK EWQFD QALP DLIR
Sbjct: 554 HPHFRFNMRINSNAREILINAGGIIESTFSAGGYSMEFSSLVYKKEWQFDTQALPEDLIR 613

Query: 619 RGMAKQKKDEEGHDVLELAIKDYPFANDGLILWNAILDWVKDYVEHYYQGDKGNKLVKND 678
           RGMA  ++D      L+LAIKDYPFANDGLILW A+++W+ +YV HYY  +K    +KND
Sbjct: 614 RGMA--ERDASAPHGLKLAIKDYPFANDGLILWEALVEWISEYVNHYYPDEKA---IKND 673

Query: 679 KELQAWWTEIKEKGHPDKKE--GWPKLETKEDLIKIVSTIAWVGSGHHSTVNFIQYAYGG 738
           KEL+AWW EI EKGHPDKK+  GWP L+T  DLI+IVSTIAWVG GHHS VNFIQYA+ G
Sbjct: 674 KELKAWWKEIMEKGHPDKKKEAGWPSLKTPNDLIQIVSTIAWVGCGHHSAVNFIQYAHAG 733

Query: 739 FIPNRPSIARINVPSEDSS---KKFINQPEEVLLKIFPSAAQGTAVATAMALLSTHSSDE 798
           + PNRPSI R N+P+ED     + FIN PE V+L+ FPS AQ + VA  M +LS HS DE
Sbjct: 734 YFPNRPSITRTNMPTEDYDQIPEAFINNPESVILEAFPSIAQASTVAQTMLILSAHSPDE 793

Query: 799 QYIGEDIKIELAWDHDNKIKVAFEKFCRKLKQLEGTIDDNNKDSEKKNRSGAGVIPYDVL 858
           + IG+  KIE AW  D  I  AF+KF  KL +LE TID  N+++E KNR GAG++PY+VL
Sbjct: 794 EDIGK--KIEPAWAEDPMIAKAFDKFKMKLDKLEKTIDQRNENNELKNRRGAGLVPYEVL 830

BLAST of Cla003209 vs. NCBI nr
Match: gi|778693083|ref|XP_011653576.1| (PREDICTED: linoleate 13S-lipoxygenase 2-1, chloroplastic [Cucumis sativus])

HSP 1 Score: 909.8 bits (2350), Expect = 3.5e-261
Identity = 480/857 (56.01%), Postives = 583/857 (68.03%), Query Frame = 1

Query: 21  GDSLPPEDTIFLTFVS-ETSHSCEKDNVVEGTVTIKKKKPGEDYNTYEGKIEVQDGFGRI 80
           G  +   D++ L F S +     ++  ++ G   + K +  +  + Y  K++V  G+G I
Sbjct: 17  GSIIHYNDSLLLFFASSDLDRDGQQKPLIRGVAELIKNESTKAKHKYVAKVQVPKGYGEI 76

Query: 81  GAVIVKISKDNSSTETFIDTVTIQKSMSDNLITFSCKSWVQPKNDDDDDDEYSQRRIFFS 140
           GAV+V++  +  S+E FIDTV +  S S N ITFSC SWVQPK+   D     QRRIFFS
Sbjct: 77  GAVVVEL--ETHSSEKFIDTVIVANSTSQNTITFSCTSWVQPKSLIPD-----QRRIFFS 136

Query: 141 TKSYLPDNTPAGLVKLRAEDLRNLRGEKPDGTIDMKERKENERIYDYDVYNDLGETDDVP 200
           TKSYLP++TPAGL+KLRAEDL NLRG+K DGT+D  ER + ERIYDYD+YNDLG+ D   
Sbjct: 137 TKSYLPEDTPAGLLKLRAEDLENLRGQKQDGTVDTNERTKFERIYDYDLYNDLGDPDVDT 196

Query: 201 MSKRHVFGGSKEYPYPRRY----------DSFETRGSGKFYVPRDEEFSKVKQSYFPDMG 260
             KR V GGS +YPYPRR              E R +  FYVPRDE FS+VKQ+ FP   
Sbjct: 197 KWKRPVLGGSVQYPYPRRCRTGRPHTVSDPKSEQRSAKSFYVPRDEAFSEVKQTMFP--S 256

Query: 261 QKNNMDELGEDHFTDLREIESLYHGGIKTPTAPHKILHLEISSILS----PHQPPVLHSA 320
              N D+LG   FTD  +I+ ++  GI  P+ PH+IL   I S L+    P  PPV    
Sbjct: 257 NSGNKDKLGTKPFTDFTQIDLMFRDGIALPSNPHEILQFNILSTLNASNQPAPPPVQPI- 316

Query: 321 ISLTTILPPAESHISEYTFYINETLKSVKITVHLPHICLLIFPRDRYNWLSDGEFARQTL 380
                   P + +     F   E LK                 RD++NWLSD EFARQT+
Sbjct: 317 --------PVQPNTPVIKFPPPEALK-----------------RDKFNWLSDSEFARQTI 376

Query: 381 AGVNPCSIQLVKSLPFESALDIHKYGPKESKFTPTWFEESMKGSITYIQAITQKKLFVLD 440
           AG+NP  IQLVKS P +S LD  +YGP+ES FT    ++ +  SIT  +AI QKKLFVLD
Sbjct: 377 AGLNPYCIQLVKSWPLKSELDPVEYGPQESAFTTELVQKLIGSSITVEEAIAQKKLFVLD 436

Query: 441 YHDILMPYVIDVRNIDNKTLYGSRTLFFLNSDDTLMPLGIELTRPPMDGDGQWKEIFKPS 500
           YHD LM YV  VR+I   TLYGSRTLFFL  DDTL P+GIELTRPPMDG  QWK++F PS
Sbjct: 437 YHDTLMQYVEKVRSIKRTTLYGSRTLFFLEYDDTLTPMGIELTRPPMDGKPQWKQVFSPS 496

Query: 501 IKGTDLWQWRFAKAHVLSHDSCIHQLVIHWLRAHCCMETYAIATNRQLSTMHPIYRLLHP 560
            +GT+ W WR AKAHVL+HDSC+HQLVIHWLR HCCME YAIA NRQLSTMHPIYRLLHP
Sbjct: 497 TQGTNAWLWRLAKAHVLAHDSCVHQLVIHWLRTHCCMEPYAIALNRQLSTMHPIYRLLHP 556

Query: 561 HFRYNMRINAQARKDLISAGGLIEGTFSTTSLSMELSSLVYKNEWQFDQQALPNDLIRRG 620
           HFR+NMRINA AR+ L +AGG+IE TFS  + SME SSLVYK EWQFD QALP DLIRRG
Sbjct: 557 HFRFNMRINANARESLNNAGGIIESTFSVGAYSMEFSSLVYKKEWQFDTQALPEDLIRRG 616

Query: 621 MAKQKKDEEGHDVLELAIKDYPFANDGLILWNAILDWVKDYVEHYYQGDKGNKLVKNDKE 680
           MA  ++D      L+LAIKDYPFANDGLILW  +++W+ +YV HYY  +K    +KNDKE
Sbjct: 617 MA--ERDASAPHGLKLAIKDYPFANDGLILWEVLVEWISEYVNHYYPDEKA---IKNDKE 676

Query: 681 LQAWWTEIKEKGHPDKKE--GWPKLETKEDLIKIVSTIAWVGSGHHSTVNFIQYAYGGFI 740
           L+AWW EI EKGHPDKK+  GWP L+T +DLI+IVSTIAWVG GHHS VNFIQYA+ G+ 
Sbjct: 677 LKAWWKEIMEKGHPDKKKEAGWPSLKTPKDLIQIVSTIAWVGCGHHSAVNFIQYAHAGYF 736

Query: 741 PNRPSIARINVPSEDSS---KKFINQPEEVLLKIFPSAAQGTAVATAMALLSTHSSDEQY 800
           PNRPSI R N+P+ED     + FIN PE V+L+ FPS AQ +AVA  M +LS HS DE+Y
Sbjct: 737 PNRPSITRTNMPTEDYDQIPEAFINNPESVILEAFPSIAQASAVAQTMLILSAHSPDEEY 796

Query: 801 IGEDIKIELAWDHDNKIKVAFEKFCRKLKQLEGTIDDNNKDSEKKNRSGAGVIPYDVLKP 858
           I +  KIE AW  D  I  AF+KF  KL +LE TID  N+++E KNR GAG++PY+VLKP
Sbjct: 797 IAK--KIEPAWAEDPMIAKAFDKFKMKLDELEKTIDQRNENNELKNRRGAGLVPYEVLKP 831

BLAST of Cla003209 vs. NCBI nr
Match: gi|778693100|ref|XP_011653578.1| (PREDICTED: LOW QUALITY PROTEIN: lipoxygenase 2, chloroplastic-like [Cucumis sativus])

HSP 1 Score: 903.7 bits (2334), Expect = 2.5e-259
Identity = 491/859 (57.16%), Postives = 583/859 (67.87%), Query Frame = 1

Query: 19  KKGDSLPPEDTIFLTFVSETSHSCEKDN-VVEGTVTIKKKKPGEDYNTYEGKIEVQDGFG 78
           KK +  P  + I+L F S    S E+    ++    +++     +Y  Y+G I+V +GFG
Sbjct: 14  KKNEQFPWVEIIYLKFASVELDSNEQPKEFIKCEAQLQEVDESVEYKKYKGDIKVAEGFG 73

Query: 79  RIGAVIVKISKDNSSTETFIDTVTIQKSMSDNLITFSCKSWVQPKNDDDDDDEYSQRRIF 138
           +IGAVIV++   +   E F+DT++++       +TFSCKSWVQPK   D       RRIF
Sbjct: 74  KIGAVIVELQ--DGVNERFVDTISVEAEKPPISVTFSCKSWVQPKGLID------HRRIF 133

Query: 139 FST-KSYLPDNTPAGLVKLRAEDLRNLRGEKPDGTIDMKERKENERIYDYDVYNDLGETD 198
           FS+ KSYL   TP GLVKLR EDL NLRGEK DG++D  ERK  ERIYDYD+YNDLG+ D
Sbjct: 134 FSSNKSYLSGKTPGGLVKLREEDLANLRGEKADGSVDRNERKAFERIYDYDLYNDLGDPD 193

Query: 199 DVPMSKRHVFGGSKEYPYPRRY----------DSFETRGSGKFYVPRDEEFSKVKQSYFP 258
                KR V GGS+EYPYPRR            + E R    FYVPRDEEFS++KQS   
Sbjct: 194 QSMNLKRPVLGGSEEYPYPRRCRTGRPLTQNDPASEKRIEEWFYVPRDEEFSEIKQS--- 253

Query: 259 DMGQKNNMDELGEDHFTDLREIESLYHGGIKTPTAPHKI-LHLEISSILSPHQPPVLHSA 318
              Q  N   L +  F+DL E+E      IKTP+A  K  L   I+SILS H+PP L S+
Sbjct: 254 -SSQPGNEKLLRKSPFSDLPELE------IKTPSAASKFNLQFNIASILSSHRPPALKSS 313

Query: 319 ---ISLTTILPPAESHISEYTFYINETLKSVKITVHLPHICLLIFPRDRYNWLSDGEFAR 378
               S    LPP ES                             + RD+YNWLSD EFAR
Sbjct: 314 DTPSSTLVELPPPES-----------------------------YKRDKYNWLSDIEFAR 373

Query: 379 QTLAGVNPCSIQLVKSLPFESALDIHKYGPKESKFTPTWFEESMKGSITYIQAITQKKLF 438
            TL G+NP SIQLVKSLPF S LD   Y P+ESKFTP   +E +   IT  +A+  K+LF
Sbjct: 374 LTLVGLNPYSIQLVKSLPFMSKLDEGDYRPRESKFTPEXVQELLGCYITVDKALADKRLF 433

Query: 439 VLDYHDILMPYVIDVRNIDNKTLYGSRTLFFLNSDDTLMPLGIELTRPPMDGD-GQWKEI 498
           V+DYHD LMPYV  VR I   TLYGSRTLFFLNSD  LMPLGIELTRPPMDG   QWKE+
Sbjct: 434 VVDYHDTLMPYVSKVRKIKGTTLYGSRTLFFLNSDGILMPLGIELTRPPMDGHLEQWKEV 493

Query: 499 FKPSIKGTDLWQWRFAKAHVLSHDSCIHQLVIHWLRAHCCMETYAIATNRQLSTMHPIYR 558
           F P    TD+W WR AKAHVLSHDSCIHQLVIHWLRAHCCME+YAIATNRQLSTMHPIYR
Sbjct: 494 FTPGTNSTDVWLWRLAKAHVLSHDSCIHQLVIHWLRAHCCMESYAIATNRQLSTMHPIYR 553

Query: 559 LLHPHFRYNMRINAQARKDLISAGGLIEGTFSTTSLSMELSSLVYKNEWQFDQQALPNDL 618
           LLHPHFRYNMRINA ARK+LI+A G+IE  FS  S S+ELSS  YK  W+FD+QALP DL
Sbjct: 554 LLHPHFRYNMRINANARKNLINARGIIENAFSGASYSVELSSSAYK-AWRFDEQALPEDL 613

Query: 619 IRRGMAKQKKDEEGHDVLELAIKDYPFANDGLILWNAILDWVKDYVEHYYQGDKGNKLVK 678
           I RGMA++K+D EG DVLEL IKDYPFANDGL+LWNA+L+WV +YV HYY GD+ N  V 
Sbjct: 614 IHRGMAERKRDSEGRDVLELTIKDYPFANDGLLLWNALLEWVTEYVNHYY-GDEEN-AVT 673

Query: 679 NDKELQAWWTEIKEKGHPDKKEGWPKLETKEDLIKIVSTIAWVGSGHHSTVNFIQYAYGG 738
           NDKELQAWW EI+EKGHPDK+EGWP L T++DLIKI STIAWVGSGHH++VNF+QYAYGG
Sbjct: 674 NDKELQAWWGEIQEKGHPDKREGWPALRTRDDLIKIASTIAWVGSGHHASVNFLQYAYGG 733

Query: 739 FIPNRPSIARINVPSEDSS----KKFINQPEEVLLKIFPSAAQGTAVATAMALLSTHSSD 798
           ++PNRP+IAR N+ +E+ S    K F N PE  L ++FPS  Q   V   M LLS HS D
Sbjct: 734 YMPNRPTIARTNMLTENHSDEILKDFRNYPENKLNELFPSVVQAAIVKQTMFLLSIHSPD 793

Query: 799 EQYIGEDIKIELAWDHDNKIKVAFEKFCRKLKQLEGTIDDNNKDSEKKNRSGAGVIPYDV 857
           E+YIG+   IE AW  D  I  AFEKF   L  LE  ID+ N++ + KNR GAG+IPY+V
Sbjct: 794 EEYIGD--AIEPAWALDQSISKAFEKFKANLTDLEKKIDELNENKDLKNRYGAGIIPYEV 820