ClCG03G003190.1 (mRNA) Watermelon (Charleston Gray)

NameClCG03G003190.1
TypemRNA
OrganismCitrullus lanatus (Watermelon (Charleston Gray))
DescriptionPectinesterase
LocationCG_Chr03 : 3698088 .. 3699562 (-)
Sequence length927
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCATCCTTGATCCAACAACTTCTTCCATGCATGAACTTTATTGTACCTGTTTGTAGTATGATGTTTAATGCTGCTGTATCGTTAGATGGAAGTGGAGAGTTTATAAGTATTGGTGCTGCTGTAGCAGCTGCACCGAACAACAGCAAGTCCAGATTTTACATTAATGTGAAACCTGGAATATATAGTGAACACATTGAAATTGAAAGTCATAAAACTTTCATAGCATTAATCGGTGATAATAATGCAGCCACCACTGTGATTGTTGATAATAGAACCAATGGCACTGGTTTTGGAACCTCCCAATCTGCTACTGTTGGTAAGCTTGAACTACCCACCGTTTTTTTTTTTTTTTTTTTTTTTGCGTTTCTATGCTTTAATTTGAATCTAGTTTCTATTTGGTATAATTTGAGTTTATCTTTTTTTTTTTGTTAAAGAAAGGATACTATATTGTGTATGTGACAAAGAATACTTAATTTTCTTTTAGTCGCTAGATTTCAAAGTGTTACATTTTTATTTTGAATTTTGAATTTTGTTCCATTTTGTCTATAAATTTCAAGATTCACACTTTTATCATTAATTTTTCACTAAATATTCATATTCAATTCTTGGTGTTAATGCGTATTGATTAATTTAAAAGTATTAAATTAATTGCAATTTTAAACTAGTTTTCACTCATGCTCAACGCTATATATTAATTCAAGTTAATAAATTTAAATTTAGTGCCAAATACCAAAAATGGTGTTTAGCAAAAACCCAAGGAAAGAAATATATATATATATATATATATATATATATATATTATTTATTTTTTTTTTATTTTTTTATTTTTTTTTTTTTTGCAGTCGTGAAGGGTAACAACTTTATGGCACAATTTATAACATTTCAAAATTCTGCTGGACCTGGAAACCACCAAGCAGTTGCTCTTCTTAATGACAATAACCAAACAACATATTTCAAGTGTGTTTTTGTGGGATACCAAGACACACTTTCTATAAATGTAAAATAATTGTTGTTCAAAGATTTGTGAAATTTATGGAACGGTAGATTTCATATTTGGAAATGGTCTTGCTGTATTTCAAGATTGCAAAATATATGCACTGTTCCCAAATAACAAGACAATTATTACCATCACCGCACAATCTAAAAATTCACAAGACCAATGTAGTGGCTTCTCTTTTCAAAATTGCAGCATCATGGCCTCTCTTGAACTTGAGTCAAGAAAGGTAAATAAGTTTGGAGTCTATCTTGGTCGACCTTGGAGAGACTATTCAACTGTAATATTCATGCAGTCATTTATGGACTATATAGTCGTCAATCCAGCAGGATGGTTAGCATGGTCAGCAGAGAGGCCTCTAAACTTATTATTTTATGCTGAGTTTAACAACACAGGTCCAGGAGCTGATTTATCTAAAAGGGCCAAATGGTTAGGCATCCATATAATAAATAAAATTTACCGTGCGAAATTTCATTAA

mRNA sequence

ATGGCATCCTTGATCCAACAACTTCTTCCATGCATGAACTTTATTGTACCTGTTTGTAGTATGATGTTTAATGCTGCTGTATCGTTAGATGGAAGTGGAGAGTTTATAAGTATTGGTGCTGCTGTAGCAGCTGCACCGAACAACAGCAAGTCCAGATTTTACATTAATGTGAAACCTGGAATATATAGTGAACACATTGAAATTGAAAGTCATAAAACTTTCATAGCATTAATCGGTGATAATAATGCAGCCACCACTGTGATTGTTGATAATAGAACCAATGGCACTGGTTTTGGAACCTCCCAATCTGCTACTGTTGTCGTGAAGGGTAACAACTTTATGGCACAATTTATAACATTTCAAAATTCTGCTGGACCTGGAAACCACCAAGCAGTTGCTCTTCTTAATGACAATAACCAAACAACATATTTCAAGTGTGTTTTTGTGGGATACCAAGACACACTTTCTATAAATATTTGTGAAATTTATGGAACGGTAGATTTCATATTTGGAAATGGTCTTGCTGTATTTCAAGATTGCAAAATATATGCACTGTTCCCAAATAACAAGACAATTATTACCATCACCGCACAATCTAAAAATTCACAAGACCAATGTAGTGGCTTCTCTTTTCAAAATTGCAGCATCATGGCCTCTCTTGAACTTGAGTCAAGAAAGGTAAATAAGTTTGGAGTCTATCTTGGTCGACCTTGGAGAGACTATTCAACTGTAATATTCATGCAGTCATTTATGGACTATATAGTCGTCAATCCAGCAGGATGGTTAGCATGGTCAGCAGAGAGGCCTCTAAACTTATTATTTTATGCTGAGTTTAACAACACAGGTCCAGGAGCTGATTTATCTAAAAGGGCCAAATGGTTAGGCATCCATATAATAAATAAAATTTACCGTGCGAAATTTCATTAA

Coding sequence (CDS)

ATGGCATCCTTGATCCAACAACTTCTTCCATGCATGAACTTTATTGTACCTGTTTGTAGTATGATGTTTAATGCTGCTGTATCGTTAGATGGAAGTGGAGAGTTTATAAGTATTGGTGCTGCTGTAGCAGCTGCACCGAACAACAGCAAGTCCAGATTTTACATTAATGTGAAACCTGGAATATATAGTGAACACATTGAAATTGAAAGTCATAAAACTTTCATAGCATTAATCGGTGATAATAATGCAGCCACCACTGTGATTGTTGATAATAGAACCAATGGCACTGGTTTTGGAACCTCCCAATCTGCTACTGTTGTCGTGAAGGGTAACAACTTTATGGCACAATTTATAACATTTCAAAATTCTGCTGGACCTGGAAACCACCAAGCAGTTGCTCTTCTTAATGACAATAACCAAACAACATATTTCAAGTGTGTTTTTGTGGGATACCAAGACACACTTTCTATAAATATTTGTGAAATTTATGGAACGGTAGATTTCATATTTGGAAATGGTCTTGCTGTATTTCAAGATTGCAAAATATATGCACTGTTCCCAAATAACAAGACAATTATTACCATCACCGCACAATCTAAAAATTCACAAGACCAATGTAGTGGCTTCTCTTTTCAAAATTGCAGCATCATGGCCTCTCTTGAACTTGAGTCAAGAAAGGTAAATAAGTTTGGAGTCTATCTTGGTCGACCTTGGAGAGACTATTCAACTGTAATATTCATGCAGTCATTTATGGACTATATAGTCGTCAATCCAGCAGGATGGTTAGCATGGTCAGCAGAGAGGCCTCTAAACTTATTATTTTATGCTGAGTTTAACAACACAGGTCCAGGAGCTGATTTATCTAAAAGGGCCAAATGGTTAGGCATCCATATAATAAATAAAATTTACCGTGCGAAATTTCATTAA

Protein sequence

MASLIQQLLPCMNFIVPVCSMMFNAAVSLDGSGEFISIGAAVAAAPNNSKSRFYINVKPGIYSEHIEIESHKTFIALIGDNNAATTVIVDNRTNGTGFGTSQSATVVVKGNNFMAQFITFQNSAGPGNHQAVALLNDNNQTTYFKCVFVGYQDTLSINICEIYGTVDFIFGNGLAVFQDCKIYALFPNNKTIITITAQSKNSQDQCSGFSFQNCSIMASLELESRKVNKFGVYLGRPWRDYSTVIFMQSFMDYIVVNPAGWLAWSAERPLNLLFYAEFNNTGPGADLSKRAKWLGIHIINKIYRAKFH
BLAST of ClCG03G003190.1 vs. Swiss-Prot
Match: PME39_ARATH (Probable pectinesterase/pectinesterase inhibitor 39 OS=Arabidopsis thaliana GN=PME39 PE=2 SV=1)

HSP 1 Score: 241.1 bits (614), Expect = 1.6e-62
Identity = 130/290 (44.83%), Postives = 178/290 (61.38%), Query Frame = 1

Query: 17  PVCSMMFNAAVSLDGSGEFISIGAAVAAAPNNSKSRFYINVKPGIYSEHIEIESHKTFIA 76
           PV    +N +V++DG+G F +I  AV AAPN S++RF I +K G Y E++E+   KT I 
Sbjct: 214 PVQETNYNLSVAIDGTGNFTTINDAVFAAPNMSETRFIIYIKGGEYFENVELPKKKTMIM 273

Query: 77  LIGDNNAATTVIVDNRTNGTGFGTSQSATVVVKGNNFMAQFITFQNSAGPGNHQAVALLN 136
            IGD     TVI  NR+   G+ T Q+ TV VKG  ++A+ I+F NSAGP   QAVA  +
Sbjct: 274 FIGDG-IGKTVIKANRSRIDGWSTFQTPTVGVKGKGYIAKDISFVNSAGPAKAQAVAFRS 333

Query: 137 DNNQTTYFKCVFVGYQDTLSINI-------CEIYGTVDFIFGNGLAVFQDCKIYALFPNN 196
            ++ + +++C F GYQDTL ++        C+IYGT+DFIFGN   VFQ+  +YA  PN 
Sbjct: 334 GSDHSAFYRCEFDGYQDTLYVHSAKQFYRECDIYGTIDFIFGNAAVVFQNSSLYARKPNP 393

Query: 197 KTIITITAQSKNSQDQCSGFSFQNCSIMASLELESRKVNKFGVYLGRPWRDYSTVIFMQS 256
              I  TAQS+N  DQ +G S  NC I+A+ +L   K N F  YLGRPWR YS  + ++S
Sbjct: 394 GHKIAFTAQSRNQSDQPTGISILNCRILAAPDLIPVKEN-FKAYLGRPWRKYSRTVIIKS 453

Query: 257 FMDYIVVNPAGWLAWSAERPLNLLFYAEFNNTGPGADLSKRAKWLGIHII 300
           F+D + ++PAGWL    +  L  L+Y E+ N GPGA+++KR  W G   I
Sbjct: 454 FIDDL-IHPAGWLEGKKDFALETLYYGEYMNEGPGANMAKRVTWPGFRRI 500

BLAST of ClCG03G003190.1 vs. Swiss-Prot
Match: PME22_ARATH (Putative pectinesterase/pectinesterase inhibitor 22 OS=Arabidopsis thaliana GN=PME22 PE=3 SV=1)

HSP 1 Score: 236.9 bits (603), Expect = 3.0e-61
Identity = 125/285 (43.86%), Postives = 172/285 (60.35%), Query Frame = 1

Query: 24  NAAVSLDGSGEFISIGAAVAAAPNNSKSRFYINVKPGIYSEHIEIESHKTFIALIGDNNA 83
           N  V++DG G++ +I  A+  APN+S  R+ I VK G+Y E+I+++  KT I L+GD   
Sbjct: 240 NTVVAIDGKGKYRTINEAINEAPNHSTKRYVIYVKKGVYKENIDLKKKKTNIMLVGDG-I 299

Query: 84  ATTVIVDNRTNGTGFGTSQSATVVVKGNNFMAQFITFQNSAGPGNHQAVALLNDNNQTTY 143
             T+I  +R    G  T ++ATV V G  F+A+ ITF+N+AGP N QAVAL  D++Q+ +
Sbjct: 300 GQTIITGDRNFMQGLTTFRTATVAVSGRGFIAKDITFRNTAGPQNRQAVALRVDSDQSAF 359

Query: 144 FKCVFVGYQDTLSINI-------CEIYGTVDFIFGNGLAVFQDCKIYALFPNNKTIITIT 203
           ++C   GYQDTL  +        CEIYGT+DFIFGNG AV Q+CKIY   P     +TIT
Sbjct: 360 YRCSVEGYQDTLYAHSLRQFYRDCEIYGTIDFIFGNGAAVLQNCKIYTRVPLPLQKVTIT 419

Query: 204 AQSKNSQDQCSGFSFQNCSIMASLELESRKVNKFGVYLGRPWRDYSTVIFMQSFMDYIVV 263
           AQ + S +Q +GF  QN  ++A+             YLGRPW+ YS  ++M ++M  + V
Sbjct: 420 AQGRKSPNQNTGFVIQNSYVLATQP----------TYLGRPWKLYSRTVYMNTYMSQL-V 479

Query: 264 NPAGWLAWSAERPLNLLFYAEFNNTGPGADLSKRAKWLGIHIINK 302
            P GWL W     L+ L+Y E+NN GPG   S R KW G HI++K
Sbjct: 480 QPRGWLEWFGNFALDTLWYGEYNNIGPGWRSSGRVKWPGYHIMDK 512

BLAST of ClCG03G003190.1 vs. Swiss-Prot
Match: PME12_ARATH (Probable pectinesterase/pectinesterase inhibitor 12 OS=Arabidopsis thaliana GN=PME12 PE=2 SV=1)

HSP 1 Score: 236.9 bits (603), Expect = 3.0e-61
Identity = 124/280 (44.29%), Postives = 172/280 (61.43%), Query Frame = 1

Query: 27  VSLDGSGEFISIGAAVAAAPNNSKSRFYINVKPGIYSEHIEIESHKTFIALIGDNNAATT 86
           V+ DG+G F +I  A++ APN S  R  I VK G+Y E+I+I  +KT I LIGD +  T 
Sbjct: 240 VAADGTGNFSTINEAISFAPNMSNDRVLIYVKEGVYDENIDIPIYKTNIVLIGDGSDVT- 299

Query: 87  VIVDNRTNGTGFGTSQSATVVVKGNNFMAQFITFQNSAGPGNHQAVALLNDNNQTTYFKC 146
            I  NR+ G G+ T +SAT+ V G  F+A+ I   N+AGP  HQAVAL  + +    ++C
Sbjct: 300 FITGNRSVGDGWTTFRSATLAVSGEGFLARDIMITNTAGPEKHQAVALRVNADFVALYRC 359

Query: 147 VFVGYQDTLSINI-------CEIYGTVDFIFGNGLAVFQDCKIYALFPNNKTIITITAQS 206
           V  GYQDTL  +        C+IYGT+D+IFGN   VFQ C I +  P       ITAQS
Sbjct: 360 VIDGYQDTLYTHSFRQFYRECDIYGTIDYIFGNAAVVFQGCNIVSKLPMPGQFTVITAQS 419

Query: 207 KNSQDQCSGFSFQNCSIMASLELESRKVNKFGVYLGRPWRDYSTVIFMQSFMDYIVVNPA 266
           +++QD+ +G S QNCSI+AS +L +   NK   YLGRPWR++S  + M+S++D   ++ +
Sbjct: 420 RDTQDEDTGISMQNCSILASEDLFNSS-NKVKSYLGRPWREFSRTVVMESYIDEF-IDGS 479

Query: 267 GWLAWSAERPLNLLFYAEFNNTGPGADLSKRAKWLGIHII 300
           GW  W+    L+ L+Y E+NN GPG++  KR  W G HI+
Sbjct: 480 GWSKWNGGEALDTLYYGEYNNNGPGSETVKRVNWPGFHIM 516

BLAST of ClCG03G003190.1 vs. Swiss-Prot
Match: PME_PRUPE (Pectinesterase/pectinesterase inhibitor PPE8B OS=Prunus persica PE=2 SV=1)

HSP 1 Score: 236.5 bits (602), Expect = 3.9e-61
Identity = 129/292 (44.18%), Postives = 178/292 (60.96%), Query Frame = 1

Query: 24  NAAVSLDGSGEFISIGAAVAAAPNNSKSRFYINVKPGIYSEHIEIESHKTFIALIGDNNA 83
           +A V+ DG+G F ++  AV AAP+ S  R+ I +K G Y E++EI+  K  + +IGD   
Sbjct: 208 DAIVAQDGTGNFTNVTDAVLAAPDYSMRRYVIYIKRGTYKENVEIKKKKWNLMMIGDGMD 267

Query: 84  ATTVIVDNRTNGTGFGTSQSATVVVKGNNFMAQFITFQNSAGPGNHQAVALLNDNNQTTY 143
           AT +I  NR+   G+ T +SAT  V G  F+A+ ITF+N+AGP  HQAVAL +D++ + +
Sbjct: 268 AT-IISGNRSFVDGWTTFRSATFAVSGRGFIARDITFENTAGPEKHQAVALRSDSDLSVF 327

Query: 144 FKCVFVGYQDTLSINI-------CEIYGTVDFIFGNGLAVFQDCKIYAL--FPNNKTIIT 203
           ++C   GYQDTL  +        C+I GTVDFIFG+   VFQ+C+I A    PN K   +
Sbjct: 328 YRCNIRGYQDTLYTHTMRQFYRDCKISGTVDFIFGDATVVFQNCQILAKKGLPNQKN--S 387

Query: 204 ITAQSKNSQDQCSGFSFQNCSIMASLELESRKVNKFGVYLGRPWRDYSTVIFMQSFMDYI 263
           ITAQ +   ++ +G S Q C+I A  +LE+  VN    YLGRPW+ YS  + MQSF+   
Sbjct: 388 ITAQGRKDPNEPTGISIQFCNITADSDLEAASVNSTPTYLGRPWKLYSRTVIMQSFLSN- 447

Query: 264 VVNPAGWLAWSAERPLNLLFYAEFNNTGPGADLSKRAKWLGIHIINKIYRAK 307
           V+ P GWL W+ +  LN LFY E+ N GPGA L  R KW G  + N+  +AK
Sbjct: 448 VIRPEGWLEWNGDFALNSLFYGEYMNYGPGAGLGSRVKWPGYQVFNESTQAK 495

BLAST of ClCG03G003190.1 vs. Swiss-Prot
Match: PME2_CITSI (Pectinesterase 2 OS=Citrus sinensis GN=PECS-2.1 PE=2 SV=1)

HSP 1 Score: 235.0 bits (598), Expect = 1.1e-60
Identity = 121/283 (42.76%), Postives = 179/283 (63.25%), Query Frame = 1

Query: 24  NAAVSLDGSGEFISIGAAVAAAPNNSKSRFYINVKPGIYSEHIEIESHKTFIALIGDNNA 83
           N  V+ DGSG   +I  AVAAA     SR+ I +K G Y+E+IE++     I  +GD   
Sbjct: 202 NIVVAQDGSGNVKTIQEAVAAASRAGGSRYVIYIKAGTYNENIEVKLKN--IMFVGDG-I 261

Query: 84  ATTVIVDNRTNGTGFGTSQSATVVVKGNNFMAQFITFQNSAGPGNHQAVALLNDNNQTTY 143
             T+I  +++ G G  T +SATV V G+NF+A+ IT +N+AGP NHQAVAL + ++ + +
Sbjct: 262 GKTIITGSKSVGGGATTFKSATVAVVGDNFIARDITIRNTAGPNNHQAVALRSGSDLSVF 321

Query: 144 FKCVFVGYQDTLSINI-------CEIYGTVDFIFGNGLAVFQDCKIYALFPNNKTIITIT 203
           ++C F GYQDTL ++        C+IYGTVDFIFGN   V Q+C I+A  P N+T  T+T
Sbjct: 322 YRCSFEGYQDTLYVHSQRQFYRECDIYGTVDFIFGNAAVVLQNCNIFARKPPNRT-NTLT 381

Query: 204 AQSKNSQDQCSGFSFQNCSIMASLELESRKVNKFGVYLGRPWRDYSTVIFMQSFMDYIVV 263
           AQ +   +Q +G    NC + A+ +L+  + +    +LGRPW+ YS  +++++F+D + +
Sbjct: 382 AQGRTDPNQSTGIIIHNCRVTAASDLKPVQ-SSVKTFLGRPWKQYSRTVYIKTFLDSL-I 441

Query: 264 NPAGWLAWSAERPLNLLFYAEFNNTGPGADLSKRAKWLGIHII 300
           NPAGW+ WS +  LN L+YAE+ NTGPG+  + R KW G H++
Sbjct: 442 NPAGWMEWSGDFALNTLYYAEYMNTGPGSSTANRVKWRGYHVL 478

BLAST of ClCG03G003190.1 vs. TrEMBL
Match: A0A0A0L1M3_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_4G260430 PE=4 SV=1)

HSP 1 Score: 309.7 bits (792), Expect = 4.0e-81
Identity = 165/286 (57.69%), Postives = 203/286 (70.98%), Query Frame = 1

Query: 23  FNAAVSLDGSGEFISIGAAVAAAPNNSKSRFYINVKPGIYSEHIEIESHKTFIALIGDNN 82
           FN+ VSLDG+  FI I  A+AAAPN S +RFYI+VKPG Y E IE+   KT IALIGD +
Sbjct: 21  FNSTVSLDGTENFIKINDAIAAAPNFSTTRFYIHVKPGTYQEIIEVPYEKTCIALIGD-D 80

Query: 83  AATTVIVDNRTNGTGFGTSQSATVVVKGNNFMAQFITFQNSAGPGNHQAVALLNDNNQTT 142
           ++TT+IV+NR+NGTG  T+ SAT+ V G+NFMAQF+TFQN+AGP   QA+ALL+    T 
Sbjct: 81  SSTTIIVNNRSNGTGSSTASSATLTVNGSNFMAQFLTFQNTAGPNEGQAIALLDQATHTA 140

Query: 143 YFKCVFVGYQDTLSINI-------CEIYGTVDFIFGNGLAVFQDCKIYA-LFPNNKTIIT 202
           Y+KCVF+GYQDTL           C+IYG+VDFIFGNGL VFQDC IYA LF      IT
Sbjct: 141 YYKCVFLGYQDTLYAGALPQFFKECDIYGSVDFIFGNGLVVFQDCNIYARLF---DVQIT 200

Query: 203 ITAQSKNSQDQCSGFSFQNCSIMASLELESRKVNKFGVYLGRPWRDYSTVIFMQSFMDYI 262
           +TAQSK S +  SGF FQNC +  S E+ S K +K  V+LGRPWR YS V+F+ SF+D  
Sbjct: 201 VTAQSKPSLNSLSGFIFQNCKVTVSPEIASSK-DKVTVFLGRPWRMYSMVVFIDSFLDN- 260

Query: 263 VVNPAGWLAWSAERPLNLLFYAEFNNTGPGADLSKRAKWLGIHIIN 301
           VV P GWL W    P NLL+YAE+NN+G GA+ S+R  W G H +N
Sbjct: 261 VVQPKGWLEWPGV-PENLLYYAEYNNSGGGANTSQRVNWPGYHALN 299

BLAST of ClCG03G003190.1 vs. TrEMBL
Match: A0A0A0LR14_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_1G049450 PE=4 SV=1)

HSP 1 Score: 307.0 bits (785), Expect = 2.6e-80
Identity = 161/311 (51.77%), Postives = 211/311 (67.85%), Query Frame = 1

Query: 9   LPC-----MNFIVPVCSMMFNAAVSLDGSGEFISIGAAVAAAPNNSKSRFYINVKPGIYS 68
           +PC      N ++ + S+ FN  VSLDGSG F  I  A+AAAPNNS +RFYI+V PG Y 
Sbjct: 7   VPCSLFCFFNLMLLIHSLSFNLTVSLDGSGNFKRISDAIAAAPNNSNTRFYIHVAPGTYH 66

Query: 69  EHIEIESHKTFIALIGDNNAATTVIVDNRTNGTGFGTSQSATVVVKGNNFMAQFITFQNS 128
           E ++I   K FIAL+GD+   TT+I+DNR+NGTGF T+ SAT+ V+G+NFMA+ +TF+NS
Sbjct: 67  ETLQIPRLKKFIALVGDD-PFTTIIIDNRSNGTGFKTNNSATLTVQGDNFMAESLTFENS 126

Query: 129 AGPGNHQAVALLNDNNQTTYFKCVFVGYQDTLSIN-------ICEIYGTVDFIFGNGLAV 188
           AGP N+QAVA+ +  N T Y+KC F+ +QDTL +N         +IYG+VDFI G G  +
Sbjct: 127 AGPQNNQAVAVFDKANHTAYYKCRFLSFQDTLYVNGKPQFFKESDIYGSVDFICGYGQVM 186

Query: 189 FQDCKIYALFPNNKTIITITAQSKNSQDQCSGFSFQNCSIMASLELESRKVNKFGVYLGR 248
           FQDC IYA  P N   IT+TAQSK      SGFSFQNC++  S E+ S K N   V+LGR
Sbjct: 187 FQDCNIYARMPINS--ITVTAQSKYILRSVSGFSFQNCTVTVSREISSNKQN-VKVFLGR 246

Query: 249 PWRDYSTVIFMQSFMDYIVVNPAGWLAWSAERPLNLLFYAEFNNTGPGADLSKRAKWLGI 308
           PW+ YS V+FM+SF+D +V +  GW+ W    P+N LFY EFNN GPGAD+SKR  W   
Sbjct: 247 PWKQYSKVVFMESFLDDVVAS-EGWVEWIGV-PVNNLFYGEFNNCGPGADVSKRVNWTSY 306

BLAST of ClCG03G003190.1 vs. TrEMBL
Match: V7B127_PHAVU (Pectinesterase (Fragment) OS=Phaseolus vulgaris GN=PHAVU_009G2224000g PE=4 SV=1)

HSP 1 Score: 260.4 bits (664), Expect = 2.8e-66
Identity = 130/281 (46.26%), Postives = 186/281 (66.19%), Query Frame = 1

Query: 27  VSLDGSGEFISIGAAVAAAPNNSKSRFYINVKPGIYSEHIEIESHKTFIALIGDNNAATT 86
           V+ DG+G F SI  A+  APNNS  R  I VK GIY E++EI S+KT I ++GD +  T 
Sbjct: 83  VAADGTGNFSSITEAINFAPNNSMGRIVIYVKEGIYEENVEIPSYKTNIVMLGDGSDVT- 142

Query: 87  VIVDNRTNGTGFGTSQSATVVVKGNNFMAQFITFQNSAGPGNHQAVALLNDNNQTTYFKC 146
           VI  NR+ G G+ T +SAT+ V G+ F+A+ I  +NSAGP  HQAVAL  + + T +++C
Sbjct: 143 VITGNRSVGDGWTTFRSATLAVSGDGFLARDIAIENSAGPEKHQAVALRVNADLTAFYRC 202

Query: 147 VFVGYQDTLSINI-------CEIYGTVDFIFGNGLAVFQDCKIYALFPNNKTIITITAQS 206
              GYQDTL ++        C+I+GT+DFIFGN  A+ QDC I +  P       ITAQS
Sbjct: 203 AIYGYQDTLYVHSFRQFYRECDIFGTIDFIFGNAAAILQDCNIISRKPLPGQFTVITAQS 262

Query: 207 KNSQDQCSGFSFQNCSIMASLELESRKVNKFGVYLGRPWRDYSTVIFMQSFMDYIVVNPA 266
           ++S D+ +G S QNCSI+A+L+L S   + F  YLGRPWR YS  ++++S++D   ++P 
Sbjct: 263 RDSPDEDTGISIQNCSIIATLDLYSNS-SSFKSYLGRPWRVYSRTVYLESYIDNF-IDPK 322

Query: 267 GWLAWSAERPLNLLFYAEFNNTGPGADLSKRAKWLGIHIIN 301
           GW  WS E+PL+ L+Y E++N GPG+ +  R +WLG H+++
Sbjct: 323 GWTKWSTEQPLDTLYYGEYDNYGPGSSIDNRVQWLGYHLMD 360

BLAST of ClCG03G003190.1 vs. TrEMBL
Match: V7AY59_PHAVU (Pectinesterase OS=Phaseolus vulgaris GN=PHAVU_009G2224000g PE=4 SV=1)

HSP 1 Score: 260.4 bits (664), Expect = 2.8e-66
Identity = 130/281 (46.26%), Postives = 186/281 (66.19%), Query Frame = 1

Query: 27  VSLDGSGEFISIGAAVAAAPNNSKSRFYINVKPGIYSEHIEIESHKTFIALIGDNNAATT 86
           V+ DG+G F SI  A+  APNNS  R  I VK GIY E++EI S+KT I ++GD +  T 
Sbjct: 100 VAADGTGNFSSITEAINFAPNNSMGRIVIYVKEGIYEENVEIPSYKTNIVMLGDGSDVT- 159

Query: 87  VIVDNRTNGTGFGTSQSATVVVKGNNFMAQFITFQNSAGPGNHQAVALLNDNNQTTYFKC 146
           VI  NR+ G G+ T +SAT+ V G+ F+A+ I  +NSAGP  HQAVAL  + + T +++C
Sbjct: 160 VITGNRSVGDGWTTFRSATLAVSGDGFLARDIAIENSAGPEKHQAVALRVNADLTAFYRC 219

Query: 147 VFVGYQDTLSINI-------CEIYGTVDFIFGNGLAVFQDCKIYALFPNNKTIITITAQS 206
              GYQDTL ++        C+I+GT+DFIFGN  A+ QDC I +  P       ITAQS
Sbjct: 220 AIYGYQDTLYVHSFRQFYRECDIFGTIDFIFGNAAAILQDCNIISRKPLPGQFTVITAQS 279

Query: 207 KNSQDQCSGFSFQNCSIMASLELESRKVNKFGVYLGRPWRDYSTVIFMQSFMDYIVVNPA 266
           ++S D+ +G S QNCSI+A+L+L S   + F  YLGRPWR YS  ++++S++D   ++P 
Sbjct: 280 RDSPDEDTGISIQNCSIIATLDLYSNS-SSFKSYLGRPWRVYSRTVYLESYIDNF-IDPK 339

Query: 267 GWLAWSAERPLNLLFYAEFNNTGPGADLSKRAKWLGIHIIN 301
           GW  WS E+PL+ L+Y E++N GPG+ +  R +WLG H+++
Sbjct: 340 GWTKWSTEQPLDTLYYGEYDNYGPGSSIDNRVQWLGYHLMD 377

BLAST of ClCG03G003190.1 vs. TrEMBL
Match: B0L3S3_TAICR (Pectinesterase OS=Taiwania cryptomerioides PE=2 SV=1)

HSP 1 Score: 255.0 bits (650), Expect = 1.2e-64
Identity = 129/297 (43.43%), Postives = 179/297 (60.27%), Query Frame = 1

Query: 16  VPVCSMMFNAAVSLDGSGEFISIGAAVAAAPNNSKSRFYINVKPGIYSEHIEIESHKTFI 75
           V V ++  NA V+ DGSG + +I AAV AAP  S +RF I VK G+Y E++EI   K F+
Sbjct: 274 VNVTNITANAVVAKDGSGHYSTISAAVDAAPEKSTTRFIIYVKKGVYQENVEIHKKKHFL 333

Query: 76  ALIGDNNAATTVIVDNRTNGTGFGTSQSATVVVKGNNFMAQFITFQNSAGPGNHQAVALL 135
             IGD    T V       G+   T  SATV V G  F+A+ +TF+N+AGP NHQAVAL 
Sbjct: 334 MFIGDGEGVTVVTASRSVRGSNHTTFHSATVAVTGKGFIARDMTFENTAGPSNHQAVALR 393

Query: 136 NDNNQTTYFKCVFVGYQDTLSINI-------CEIYGTVDFIFGNGLAVFQDCKIYALFPN 195
             ++ + +++C F GYQDTL ++        C+IYGTVDFIFGN   VFQ+C +YA  P 
Sbjct: 394 VGSDFSVFYRCSFKGYQDTLYVHSLRQFFRDCDIYGTVDFIFGNAAVVFQNCNLYARKPL 453

Query: 196 NKTIITITAQSKNSQDQCSGFSFQNCSIMASLELESRKVNKFGVYLGRPWRDYSTVIFMQ 255
               I  TAQ +   +Q +G S  NC + A  ++ + K + F  YLGRPW++YS  +F+Q
Sbjct: 454 ENQQIMYTAQGRQDPNQNTGISIHNCRVTADSDMAAVK-SSFKTYLGRPWKEYSRTVFLQ 513

Query: 256 SFMDYIVVNPAGWLAWSAERPLNLLFYAEFNNTGPGADLSKRAKWLGIHIINKIYRA 306
           S++D + ++PAGWL W+    L+ L+Y E+ NTGPGA  + R  W G  +I     A
Sbjct: 514 SYLDDL-IHPAGWLEWNETFALSTLYYGEYMNTGPGAGTANRVNWPGYRVITSATEA 568

BLAST of ClCG03G003190.1 vs. TAIR10
Match: AT4G02300.1 (AT4G02300.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 241.1 bits (614), Expect = 8.9e-64
Identity = 130/290 (44.83%), Postives = 178/290 (61.38%), Query Frame = 1

Query: 17  PVCSMMFNAAVSLDGSGEFISIGAAVAAAPNNSKSRFYINVKPGIYSEHIEIESHKTFIA 76
           PV    +N +V++DG+G F +I  AV AAPN S++RF I +K G Y E++E+   KT I 
Sbjct: 214 PVQETNYNLSVAIDGTGNFTTINDAVFAAPNMSETRFIIYIKGGEYFENVELPKKKTMIM 273

Query: 77  LIGDNNAATTVIVDNRTNGTGFGTSQSATVVVKGNNFMAQFITFQNSAGPGNHQAVALLN 136
            IGD     TVI  NR+   G+ T Q+ TV VKG  ++A+ I+F NSAGP   QAVA  +
Sbjct: 274 FIGDG-IGKTVIKANRSRIDGWSTFQTPTVGVKGKGYIAKDISFVNSAGPAKAQAVAFRS 333

Query: 137 DNNQTTYFKCVFVGYQDTLSINI-------CEIYGTVDFIFGNGLAVFQDCKIYALFPNN 196
            ++ + +++C F GYQDTL ++        C+IYGT+DFIFGN   VFQ+  +YA  PN 
Sbjct: 334 GSDHSAFYRCEFDGYQDTLYVHSAKQFYRECDIYGTIDFIFGNAAVVFQNSSLYARKPNP 393

Query: 197 KTIITITAQSKNSQDQCSGFSFQNCSIMASLELESRKVNKFGVYLGRPWRDYSTVIFMQS 256
              I  TAQS+N  DQ +G S  NC I+A+ +L   K N F  YLGRPWR YS  + ++S
Sbjct: 394 GHKIAFTAQSRNQSDQPTGISILNCRILAAPDLIPVKEN-FKAYLGRPWRKYSRTVIIKS 453

Query: 257 FMDYIVVNPAGWLAWSAERPLNLLFYAEFNNTGPGADLSKRAKWLGIHII 300
           F+D + ++PAGWL    +  L  L+Y E+ N GPGA+++KR  W G   I
Sbjct: 454 FIDDL-IHPAGWLEGKKDFALETLYYGEYMNEGPGANMAKRVTWPGFRRI 500

BLAST of ClCG03G003190.1 vs. TAIR10
Match: AT3G05620.1 (AT3G05620.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 236.9 bits (603), Expect = 1.7e-62
Identity = 125/285 (43.86%), Postives = 172/285 (60.35%), Query Frame = 1

Query: 24  NAAVSLDGSGEFISIGAAVAAAPNNSKSRFYINVKPGIYSEHIEIESHKTFIALIGDNNA 83
           N  V++DG G++ +I  A+  APN+S  R+ I VK G+Y E+I+++  KT I L+GD   
Sbjct: 240 NTVVAIDGKGKYRTINEAINEAPNHSTKRYVIYVKKGVYKENIDLKKKKTNIMLVGDG-I 299

Query: 84  ATTVIVDNRTNGTGFGTSQSATVVVKGNNFMAQFITFQNSAGPGNHQAVALLNDNNQTTY 143
             T+I  +R    G  T ++ATV V G  F+A+ ITF+N+AGP N QAVAL  D++Q+ +
Sbjct: 300 GQTIITGDRNFMQGLTTFRTATVAVSGRGFIAKDITFRNTAGPQNRQAVALRVDSDQSAF 359

Query: 144 FKCVFVGYQDTLSINI-------CEIYGTVDFIFGNGLAVFQDCKIYALFPNNKTIITIT 203
           ++C   GYQDTL  +        CEIYGT+DFIFGNG AV Q+CKIY   P     +TIT
Sbjct: 360 YRCSVEGYQDTLYAHSLRQFYRDCEIYGTIDFIFGNGAAVLQNCKIYTRVPLPLQKVTIT 419

Query: 204 AQSKNSQDQCSGFSFQNCSIMASLELESRKVNKFGVYLGRPWRDYSTVIFMQSFMDYIVV 263
           AQ + S +Q +GF  QN  ++A+             YLGRPW+ YS  ++M ++M  + V
Sbjct: 420 AQGRKSPNQNTGFVIQNSYVLATQP----------TYLGRPWKLYSRTVYMNTYMSQL-V 479

Query: 264 NPAGWLAWSAERPLNLLFYAEFNNTGPGADLSKRAKWLGIHIINK 302
            P GWL W     L+ L+Y E+NN GPG   S R KW G HI++K
Sbjct: 480 QPRGWLEWFGNFALDTLWYGEYNNIGPGWRSSGRVKWPGYHIMDK 512

BLAST of ClCG03G003190.1 vs. TAIR10
Match: AT2G26440.1 (AT2G26440.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 236.9 bits (603), Expect = 1.7e-62
Identity = 124/280 (44.29%), Postives = 172/280 (61.43%), Query Frame = 1

Query: 27  VSLDGSGEFISIGAAVAAAPNNSKSRFYINVKPGIYSEHIEIESHKTFIALIGDNNAATT 86
           V+ DG+G F +I  A++ APN S  R  I VK G+Y E+I+I  +KT I LIGD +  T 
Sbjct: 240 VAADGTGNFSTINEAISFAPNMSNDRVLIYVKEGVYDENIDIPIYKTNIVLIGDGSDVT- 299

Query: 87  VIVDNRTNGTGFGTSQSATVVVKGNNFMAQFITFQNSAGPGNHQAVALLNDNNQTTYFKC 146
            I  NR+ G G+ T +SAT+ V G  F+A+ I   N+AGP  HQAVAL  + +    ++C
Sbjct: 300 FITGNRSVGDGWTTFRSATLAVSGEGFLARDIMITNTAGPEKHQAVALRVNADFVALYRC 359

Query: 147 VFVGYQDTLSINI-------CEIYGTVDFIFGNGLAVFQDCKIYALFPNNKTIITITAQS 206
           V  GYQDTL  +        C+IYGT+D+IFGN   VFQ C I +  P       ITAQS
Sbjct: 360 VIDGYQDTLYTHSFRQFYRECDIYGTIDYIFGNAAVVFQGCNIVSKLPMPGQFTVITAQS 419

Query: 207 KNSQDQCSGFSFQNCSIMASLELESRKVNKFGVYLGRPWRDYSTVIFMQSFMDYIVVNPA 266
           +++QD+ +G S QNCSI+AS +L +   NK   YLGRPWR++S  + M+S++D   ++ +
Sbjct: 420 RDTQDEDTGISMQNCSILASEDLFNSS-NKVKSYLGRPWREFSRTVVMESYIDEF-IDGS 479

Query: 267 GWLAWSAERPLNLLFYAEFNNTGPGADLSKRAKWLGIHII 300
           GW  W+    L+ L+Y E+NN GPG++  KR  W G HI+
Sbjct: 480 GWSKWNGGEALDTLYYGEYNNNGPGSETVKRVNWPGFHIM 516

BLAST of ClCG03G003190.1 vs. TAIR10
Match: AT1G02810.1 (AT1G02810.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 235.0 bits (598), Expect = 6.4e-62
Identity = 129/291 (44.33%), Postives = 172/291 (59.11%), Query Frame = 1

Query: 27  VSLDGSGEFISIGAAVAAAPNN---SKSRFYINVKPGIYSEHIEIESHKTFIALIGDNNA 86
           VS DG+G F +I AAVAAAPNN   S   F I V  GIY E+I I  +K ++ +IGD   
Sbjct: 267 VSQDGTGNFTNITAAVAAAPNNTDGSAGFFLIYVTAGIYEEYISIAKNKRYMMMIGDG-I 326

Query: 87  ATTVIVDNRTNGTGFGTSQSATVVVKGNNFMAQFITFQNSAGPGNHQAVALLNDNNQTTY 146
             TV+  NR+   G+ T  SAT  V   NF+A  ITF+N+AGP  HQAVAL +  + + +
Sbjct: 327 NQTVVTGNRSVVDGWTTFNSATFAVTAPNFVAVNITFRNTAGPEKHQAVALRSGADFSIF 386

Query: 147 FKCVFVGYQDTLSINI-------CEIYGTVDFIFGNGLAVFQDCKIYALFPNNKTIITIT 206
           + C F  YQDTL  +        C++YGTVDFIFGN   VFQ+C +Y   P       IT
Sbjct: 387 YSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVFQNCNLYPRKPMPNQFNAIT 446

Query: 207 AQSKNSQDQCSGFSFQNCSIMASLELESRKVNKFGVYLGRPWRDYSTVIFMQSFMDYIVV 266
           AQ ++  +Q +G S QNC+I  + +L S        YLGRPW++YS  ++MQS++D   V
Sbjct: 447 AQGRSDPNQNTGTSIQNCTIKPADDLVSSNYT-VKTYLGRPWKEYSRTVYMQSYIDGF-V 506

Query: 267 NPAGWLAWSAERPLNLLFYAEFNNTGPGADLSKRAKWLGIHIINKIYRAKF 308
            P GW  W+ +  L+ L+YAE+NNTGPG++ + R  W G H+IN    A F
Sbjct: 507 EPVGWREWNGDFALSTLYYAEYNNTGPGSNTTNRVTWPGYHVINSTDAANF 554

BLAST of ClCG03G003190.1 vs. TAIR10
Match: AT4G33220.1 (AT4G33220.1 pectin methylesterase 44)

HSP 1 Score: 234.2 bits (596), Expect = 1.1e-61
Identity = 132/287 (45.99%), Postives = 176/287 (61.32%), Query Frame = 1

Query: 23  FNAAVSLDGSGEFISIGAAVAAAPNNSKSRFYINVKPGIYSEHIEIESHKTFIALIGDNN 82
           ++ +V+LDG+G F  I  A+  AP+ S +RF I +K G+Y E++EI+  K  I ++GD  
Sbjct: 211 YDVSVALDGTGNFTKIMDAIKKAPDYSSTRFVIYIKKGLYLENVEIKKKKWNIVMLGDG- 270

Query: 83  AATTVIVDNRTNGTGFGTSQSATVVVKGNNFMAQFITFQNSAGPGNHQAVALLNDNNQTT 142
              TVI  NR+   G+ T +SAT  V G  F+A+ ITFQN+AGP  HQAVAL +D++ + 
Sbjct: 271 IDVTVISGNRSFIDGWTTFRSATFAVSGRGFLARDITFQNTAGPEKHQAVALRSDSDLSV 330

Query: 143 YFKCVFVGYQDTLSINI-------CEIYGTVDFIFGNGLAVFQDCKIYAL--FPNNKTII 202
           +F+C   GYQDTL  +        C I GTVDFIFG+G  VFQ+C+I A    PN K   
Sbjct: 331 FFRCAMRGYQDTLYTHTMRQFYRECTITGTVDFIFGDGTVVFQNCQILAKRGLPNQKN-- 390

Query: 203 TITAQSKNSQDQCSGFSFQNCSIMASLELESRKVNKFGVYLGRPWRDYSTVIFMQSFMDY 262
           TITAQ +   +Q SGFS Q  +I A  +L    +N    YLGRPW+ YS  +F+++ M  
Sbjct: 391 TITAQGRKDVNQPSGFSIQFSNISADADLVP-YLNTTRTYLGRPWKLYSRTVFIRNNMS- 450

Query: 263 IVVNPAGWLAWSAERPLNLLFYAEFNNTGPGADLSKRAKWLGIHIIN 301
            VV P GWL W+A+  L+ LFY EF N GPG+ LS R KW G H+ N
Sbjct: 451 DVVRPEGWLEWNADFALDTLFYGEFMNYGPGSGLSSRVKWPGYHVFN 492

BLAST of ClCG03G003190.1 vs. NCBI nr
Match: gi|778697621|ref|XP_011654361.1| (PREDICTED: probable pectinesterase/pectinesterase inhibitor 39 [Cucumis sativus])

HSP 1 Score: 309.7 bits (792), Expect = 5.8e-81
Identity = 165/286 (57.69%), Postives = 203/286 (70.98%), Query Frame = 1

Query: 23  FNAAVSLDGSGEFISIGAAVAAAPNNSKSRFYINVKPGIYSEHIEIESHKTFIALIGDNN 82
           FN+ VSLDG+  FI I  A+AAAPN S +RFYI+VKPG Y E IE+   KT IALIGD +
Sbjct: 28  FNSTVSLDGTENFIKINDAIAAAPNFSTTRFYIHVKPGTYQEIIEVPYEKTCIALIGD-D 87

Query: 83  AATTVIVDNRTNGTGFGTSQSATVVVKGNNFMAQFITFQNSAGPGNHQAVALLNDNNQTT 142
           ++TT+IV+NR+NGTG  T+ SAT+ V G+NFMAQF+TFQN+AGP   QA+ALL+    T 
Sbjct: 88  SSTTIIVNNRSNGTGSSTASSATLTVNGSNFMAQFLTFQNTAGPNEGQAIALLDQATHTA 147

Query: 143 YFKCVFVGYQDTLSINI-------CEIYGTVDFIFGNGLAVFQDCKIYA-LFPNNKTIIT 202
           Y+KCVF+GYQDTL           C+IYG+VDFIFGNGL VFQDC IYA LF      IT
Sbjct: 148 YYKCVFLGYQDTLYAGALPQFFKECDIYGSVDFIFGNGLVVFQDCNIYARLF---DVQIT 207

Query: 203 ITAQSKNSQDQCSGFSFQNCSIMASLELESRKVNKFGVYLGRPWRDYSTVIFMQSFMDYI 262
           +TAQSK S +  SGF FQNC +  S E+ S K +K  V+LGRPWR YS V+F+ SF+D  
Sbjct: 208 VTAQSKPSLNSLSGFIFQNCKVTVSPEIASSK-DKVTVFLGRPWRMYSMVVFIDSFLDN- 267

Query: 263 VVNPAGWLAWSAERPLNLLFYAEFNNTGPGADLSKRAKWLGIHIIN 301
           VV P GWL W    P NLL+YAE+NN+G GA+ S+R  W G H +N
Sbjct: 268 VVQPKGWLEWPGV-PENLLYYAEYNNSGGGANTSQRVNWPGYHALN 306

BLAST of ClCG03G003190.1 vs. NCBI nr
Match: gi|700198848|gb|KGN54006.1| (hypothetical protein Csa_4G260430 [Cucumis sativus])

HSP 1 Score: 309.7 bits (792), Expect = 5.8e-81
Identity = 165/286 (57.69%), Postives = 203/286 (70.98%), Query Frame = 1

Query: 23  FNAAVSLDGSGEFISIGAAVAAAPNNSKSRFYINVKPGIYSEHIEIESHKTFIALIGDNN 82
           FN+ VSLDG+  FI I  A+AAAPN S +RFYI+VKPG Y E IE+   KT IALIGD +
Sbjct: 21  FNSTVSLDGTENFIKINDAIAAAPNFSTTRFYIHVKPGTYQEIIEVPYEKTCIALIGD-D 80

Query: 83  AATTVIVDNRTNGTGFGTSQSATVVVKGNNFMAQFITFQNSAGPGNHQAVALLNDNNQTT 142
           ++TT+IV+NR+NGTG  T+ SAT+ V G+NFMAQF+TFQN+AGP   QA+ALL+    T 
Sbjct: 81  SSTTIIVNNRSNGTGSSTASSATLTVNGSNFMAQFLTFQNTAGPNEGQAIALLDQATHTA 140

Query: 143 YFKCVFVGYQDTLSINI-------CEIYGTVDFIFGNGLAVFQDCKIYA-LFPNNKTIIT 202
           Y+KCVF+GYQDTL           C+IYG+VDFIFGNGL VFQDC IYA LF      IT
Sbjct: 141 YYKCVFLGYQDTLYAGALPQFFKECDIYGSVDFIFGNGLVVFQDCNIYARLF---DVQIT 200

Query: 203 ITAQSKNSQDQCSGFSFQNCSIMASLELESRKVNKFGVYLGRPWRDYSTVIFMQSFMDYI 262
           +TAQSK S +  SGF FQNC +  S E+ S K +K  V+LGRPWR YS V+F+ SF+D  
Sbjct: 201 VTAQSKPSLNSLSGFIFQNCKVTVSPEIASSK-DKVTVFLGRPWRMYSMVVFIDSFLDN- 260

Query: 263 VVNPAGWLAWSAERPLNLLFYAEFNNTGPGADLSKRAKWLGIHIIN 301
           VV P GWL W    P NLL+YAE+NN+G GA+ S+R  W G H +N
Sbjct: 261 VVQPKGWLEWPGV-PENLLYYAEYNNSGGGANTSQRVNWPGYHALN 299

BLAST of ClCG03G003190.1 vs. NCBI nr
Match: gi|778708551|ref|XP_011656233.1| (PREDICTED: probable pectinesterase/pectinesterase inhibitor 39 [Cucumis sativus])

HSP 1 Score: 308.9 bits (790), Expect = 9.9e-81
Identity = 165/287 (57.49%), Postives = 202/287 (70.38%), Query Frame = 1

Query: 22  MFNAAVSLDGSGEFISIGAAVAAAPNNSKSRFYINVKPGIYSEHIEIESHKTFIALIGDN 81
           +FN  VSLDG+G F+ I  A+AAAPN S +RFYI+VKPG Y E IE+   KT IALIGD 
Sbjct: 27  IFNFTVSLDGTGNFVKINDAIAAAPNFSTTRFYIHVKPGTYKEIIEVPYEKTCIALIGD- 86

Query: 82  NAATTVIVDNRTNGTGFGTSQSATVVVKGNNFMAQFITFQNSAGPGNHQAVALLNDNNQT 141
           +A+TT+IV+NR+NGTG  T+ SAT+ V G NFMAQF+TFQN+AGP   QA+ALL+    T
Sbjct: 87  DASTTIIVNNRSNGTGSSTASSATLTVNGANFMAQFLTFQNTAGPNEGQAIALLDQATHT 146

Query: 142 TYFKCVFVGYQDTLSINI-------CEIYGTVDFIFGNGLAVFQDCKIYA-LFPNNKTII 201
            Y+KCVF+GYQDTL           C+IYG+VDFIFGNGL VFQDC IYA LF      I
Sbjct: 147 AYYKCVFLGYQDTLYAGALPQFFKECDIYGSVDFIFGNGLVVFQDCNIYARLF---DVQI 206

Query: 202 TITAQSKNSQDQCSGFSFQNCSIMASLELESRKVNKFGVYLGRPWRDYSTVIFMQSFMDY 261
           T+TAQSK S +  SGF FQNC++  S E+   K N   V+LGRPWR YS V+F+ SF+D 
Sbjct: 207 TVTAQSKPSLNSLSGFIFQNCNVTVSPEIAPSKDN-VTVFLGRPWRMYSMVVFIDSFLDN 266

Query: 262 IVVNPAGWLAWSAERPLNLLFYAEFNNTGPGADLSKRAKWLGIHIIN 301
            VV P GWL W    P NLL+YAE+NN+G GA+ S+R  W G H +N
Sbjct: 267 -VVQPKGWLEWPGV-PENLLYYAEYNNSGGGANTSQRVNWPGYHALN 306

BLAST of ClCG03G003190.1 vs. NCBI nr
Match: gi|778657958|ref|XP_004152582.2| (PREDICTED: pectinesterase 2-like [Cucumis sativus])

HSP 1 Score: 307.0 bits (785), Expect = 3.7e-80
Identity = 161/311 (51.77%), Postives = 211/311 (67.85%), Query Frame = 1

Query: 9   LPC-----MNFIVPVCSMMFNAAVSLDGSGEFISIGAAVAAAPNNSKSRFYINVKPGIYS 68
           +PC      N ++ + S+ FN  VSLDGSG F  I  A+AAAPNNS +RFYI+V PG Y 
Sbjct: 7   VPCSLFCFFNLMLLIHSLSFNLTVSLDGSGNFKRISDAIAAAPNNSNTRFYIHVAPGTYH 66

Query: 69  EHIEIESHKTFIALIGDNNAATTVIVDNRTNGTGFGTSQSATVVVKGNNFMAQFITFQNS 128
           E ++I   K FIAL+GD+   TT+I+DNR+NGTGF T+ SAT+ V+G+NFMA+ +TF+NS
Sbjct: 67  ETLQIPRLKKFIALVGDD-PFTTIIIDNRSNGTGFKTNNSATLTVQGDNFMAESLTFENS 126

Query: 129 AGPGNHQAVALLNDNNQTTYFKCVFVGYQDTLSIN-------ICEIYGTVDFIFGNGLAV 188
           AGP N+QAVA+ +  N T Y+KC F+ +QDTL +N         +IYG+VDFI G G  +
Sbjct: 127 AGPQNNQAVAVFDKANHTAYYKCRFLSFQDTLYVNGKPQFFKESDIYGSVDFICGYGQVM 186

Query: 189 FQDCKIYALFPNNKTIITITAQSKNSQDQCSGFSFQNCSIMASLELESRKVNKFGVYLGR 248
           FQDC IYA  P N   IT+TAQSK      SGFSFQNC++  S E+ S K N   V+LGR
Sbjct: 187 FQDCNIYARMPINS--ITVTAQSKYILRSVSGFSFQNCTVTVSREISSNKQN-VKVFLGR 246

Query: 249 PWRDYSTVIFMQSFMDYIVVNPAGWLAWSAERPLNLLFYAEFNNTGPGADLSKRAKWLGI 308
           PW+ YS V+FM+SF+D +V +  GW+ W    P+N LFY EFNN GPGAD+SKR  W   
Sbjct: 247 PWKQYSKVVFMESFLDDVVAS-EGWVEWIGV-PVNNLFYGEFNNCGPGADVSKRVNWTSY 306

BLAST of ClCG03G003190.1 vs. NCBI nr
Match: gi|659131731|ref|XP_008465826.1| (PREDICTED: probable pectinesterase/pectinesterase inhibitor 39 [Cucumis melo])

HSP 1 Score: 306.6 bits (784), Expect = 4.9e-80
Identity = 160/282 (56.74%), Postives = 203/282 (71.99%), Query Frame = 1

Query: 27  VSLDGSGEFISIGAAVAAAPNNSKSRFYINVKPGIYSEHIEIESHKTFIALIGDNNAATT 86
           +SLDG+G F+ +  A+AAAPN S +RFYI+VKPG Y E IE+ S KTFIALIGD +++TT
Sbjct: 19  LSLDGTGNFVKVNDAIAAAPNFSMTRFYIHVKPGTYQEIIEVPSEKTFIALIGD-DSSTT 78

Query: 87  VIVDNRTNGTGFGTSQSATVVVKGNNFMAQFITFQNSAGPGNHQAVALLNDNNQTTYFKC 146
           +IV+NR+NGTG  T+ SAT+ V G NFMAQF+TFQNSAGP   QA+A+L+    T Y++C
Sbjct: 79  IIVNNRSNGTGSSTASSATLTVYGENFMAQFLTFQNSAGPNEGQAIAVLDQAKHTAYYRC 138

Query: 147 VFVGYQDTL-------SINICEIYGTVDFIFGNGLAVFQDCKIYA-LFPNNKTIITITAQ 206
           VF+GYQDTL           C+IYG+VDFIFGNGL VFQDC IYA LF      IT+TAQ
Sbjct: 139 VFLGYQDTLYAGSVPQFFKECDIYGSVDFIFGNGLVVFQDCNIYARLF---DVQITVTAQ 198

Query: 207 SKNSQDQCSGFSFQNCSIMASLELESRKVNKFGVYLGRPWRDYSTVIFMQSFMDYIVVNP 266
           SK S    SGF FQNC++  S E+ S K +K  V+LGRPWR YS V+F+ SF+D  VV P
Sbjct: 199 SKQSLSAPSGFIFQNCNVTVSPEIASSK-DKVTVFLGRPWRMYSMVVFIDSFLD-SVVQP 258

Query: 267 AGWLAWSAERPLNLLFYAEFNNTGPGADLSKRAKWLGIHIIN 301
            GWL W    P N+L+YAE+NN+G GA+ S+R  W G H+++
Sbjct: 259 KGWLEWPGV-PENVLYYAEYNNSGGGANTSQRVNWPGYHVLD 293

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
PME39_ARATH1.6e-6244.83Probable pectinesterase/pectinesterase inhibitor 39 OS=Arabidopsis thaliana GN=P... [more]
PME22_ARATH3.0e-6143.86Putative pectinesterase/pectinesterase inhibitor 22 OS=Arabidopsis thaliana GN=P... [more]
PME12_ARATH3.0e-6144.29Probable pectinesterase/pectinesterase inhibitor 12 OS=Arabidopsis thaliana GN=P... [more]
PME_PRUPE3.9e-6144.18Pectinesterase/pectinesterase inhibitor PPE8B OS=Prunus persica PE=2 SV=1[more]
PME2_CITSI1.1e-6042.76Pectinesterase 2 OS=Citrus sinensis GN=PECS-2.1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0L1M3_CUCSA4.0e-8157.69Pectinesterase OS=Cucumis sativus GN=Csa_4G260430 PE=4 SV=1[more]
A0A0A0LR14_CUCSA2.6e-8051.77Pectinesterase OS=Cucumis sativus GN=Csa_1G049450 PE=4 SV=1[more]
V7B127_PHAVU2.8e-6646.26Pectinesterase (Fragment) OS=Phaseolus vulgaris GN=PHAVU_009G2224000g PE=4 SV=1[more]
V7AY59_PHAVU2.8e-6646.26Pectinesterase OS=Phaseolus vulgaris GN=PHAVU_009G2224000g PE=4 SV=1[more]
B0L3S3_TAICR1.2e-6443.43Pectinesterase OS=Taiwania cryptomerioides PE=2 SV=1[more]
Match NameE-valueIdentityDescription
AT4G02300.18.9e-6444.83 Plant invertase/pectin methylesterase inhibitor superfamily[more]
AT3G05620.11.7e-6243.86 Plant invertase/pectin methylesterase inhibitor superfamily[more]
AT2G26440.11.7e-6244.29 Plant invertase/pectin methylesterase inhibitor superfamily[more]
AT1G02810.16.4e-6244.33 Plant invertase/pectin methylesterase inhibitor superfamily[more]
AT4G33220.11.1e-6145.99 pectin methylesterase 44[more]
Match NameE-valueIdentityDescription
gi|778697621|ref|XP_011654361.1|5.8e-8157.69PREDICTED: probable pectinesterase/pectinesterase inhibitor 39 [Cucumis sativus][more]
gi|700198848|gb|KGN54006.1|5.8e-8157.69hypothetical protein Csa_4G260430 [Cucumis sativus][more]
gi|778708551|ref|XP_011656233.1|9.9e-8157.49PREDICTED: probable pectinesterase/pectinesterase inhibitor 39 [Cucumis sativus][more]
gi|778657958|ref|XP_004152582.2|3.7e-8051.77PREDICTED: pectinesterase 2-like [Cucumis sativus][more]
gi|659131731|ref|XP_008465826.1|4.9e-8056.74PREDICTED: probable pectinesterase/pectinesterase inhibitor 39 [Cucumis melo][more]
The following terms have been associated with this mRNA:
Vocabulary: INTERPRO
TermDefinition
IPR000070Pectinesterase_cat
IPR011050Pectin_lyase_fold/virulence
IPR012334Pectin_lyas_fold
Vocabulary: Cellular Component
TermDefinition
GO:0005618cell wall
Vocabulary: Molecular Function
TermDefinition
GO:0030599pectinesterase activity
Vocabulary: Biological Process
TermDefinition
GO:0042545cell wall modification
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0005982 starch metabolic process
biological_process GO:0005985 sucrose metabolic process
cellular_component GO:0005618 cell wall
molecular_function GO:0030599 pectinesterase activity

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
ClCG03G003190ClCG03G003190gene


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
ClCG03G003190.1ClCG03G003190.1-proteinpolypeptide


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
ClCG03G003190.1.cds3ClCG03G003190.1.cds3CDS
ClCG03G003190.1.cds2ClCG03G003190.1.cds2CDS
ClCG03G003190.1.cds1ClCG03G003190.1.cds1CDS


Analysis Name: InterPro Annotations of watermelon (Charleston Gray)
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 25..302
score: 6.6
IPR011050Pectin lyase fold/virulence factorunknownSSF51126Pectin lyase-likecoord: 26..300
score: 1.26
IPR012334Pectin lyase foldGENE3DG3DSA:2.160.20.10coord: 24..297
score: 8.3
NoneNo IPR availablePANTHERPTHR31707FAMILY NOT NAMEDcoord: 20..300
score: 9.1E