Carg25777-RA (mRNA) Silver-seed gourd

NameCarg25777-RA
TypemRNA
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionPhospholipase a1-igamma2, chloroplastic
LocationCucurbita_argyrosperma_scaffold_318 : 72743 .. 75211 (+)
Sequence length1804
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGATTCACCTCTCCAATGTCCTCTTCTTCCCAATCACAAACTCACTCCACCAATCCAAACAATCATCTTCTTCACTTCATTTCTCAACCTCAAATCGTCACTTTCGTGGTTTCAGATTATTCAGTACAAGGAGAACACAACGACGACGACAATCGACATACGAAGTAGCTTCAAGAACCAGAGACACATCCTCCATTATTACAGACCTCGAAAAACAAACAGAGGGAGATGAACATACCGAAAACGAATCAGTGGCAGCAGAAATCGCTAATTCCTGGCGTGAAATCCATGGCAGCAACGACTGGACCGGGTTGCTTGATCCGATGAACGATCTCCTCCGCTCCGAGATAATCCGCTACGGTGAAATGGCGCAATCCTGCTACGACGCTTTTGATTACGATCCGTTCTCGAAATACTGCGGGAGTTGTCGATTTAGTCGAAGCAAATTCTTCGAACGACTCGGAATGGAAAGCGGAGGTTACGAAGTAACGCGGTACCTCTACGCAACATCAAACATCAACCTACCGAATTTCTTCAAGAAATCGCGATGGCCAAAAGTATGGAGCAAGAACGCGAACTGGATCGGCTACGTCGCGGTATCAAACGACGCGCAATCGAAACTCCTCGGCCGGCGAGACATCGCCATAGCGTGGAGAGGCACCGTAACGCGGTTGGAATGGATCGTGGATCTAATGGATTTTCTGAAACCAGTAGCGGCGGTGAAGATTCCATGCCCTAATTTGGCGGTGAAGGCGGAATCGGGATTTCTGGCTCTGTATACAGACAAGGACGAAGGTTGTGGATACAGCAAGTTGTCAGCGAGAGAGCAGATTGTGACGGAGGTGAAGCGGTTGGTGGAGAGATTCGCCGGAGAAGAGATGAGCATTACGATCACTGGACACAGTTTGGGAAGCGCTCTGGCGGTGTTGAGCGGCTTCGACGTTGCGGAAATGGGTGTGAACCGGTTGGAGAACGGTCGGGTTGTACCGGTTTCGGTTTTCTCATTCTCGGGGCCCCGAGTTGGGAACTTCTCCTTCAAGGAACGCCTCCAAGAATTGGGAGTTAAGGTAATTAATCTTAATTAATTAATTAATTAATTAATAATTAGTGGAAATAATAATATTAAAATGAAAACAGGTATTGAGAGTGGTGAATGTCCATGACGTGGTACCCAAGACGCCAGGATTTTTATTCAACGAAAGCGTACCAACGGCTGTGATGAAATTTGCGGAGGAATTACCGTGGAGTTATTCACACGTGGGCGTGGAGTTAAAATTGGATCATAAAATTTCACCGTTCCTCAAGGATACAAACGACCCCGTTTGTGCACATAATTTGGAAGCTCACTTACATTTACTTGATGGGTAACTTTCTGACCTTTTTCTTTTTATCTTCCTATATTATACTAATTACCATTCATTTTGCTAATTCTATATCATGTGAAATCATCATTTTTAGCTTAATAAATATGTCACATAATTATTTAAATAACAATATCAAATGGTACTTGTTAAAATACCCTTTGATATAAATGAAATAAAAATATAATAAATTAATAACCATAATTCTATATATATATATATATATATATTAAAATAATATTATCTTCTAGGGTATCTAAAATGATTGAACAGGATAATATTATATCACCAAATTTTCAATATATATATATATATTAATAAAAAAAATCAAGGATTTGGGATATGTTTCTGACATTATTATATTGTATTATTTCTTTACAATATTTGATGAATATATTATATTAAACTGTGACGATAGGGACAATTATGCATTATGATAAGTGAGAATTTTATATGGTAGTTTGGTAGGATGATCGAGTTTGGTAGCACGTTGAAGAGAAATGTTTTGTTAAATTACAAAATTTGTTCTAATTTTAATAATTAGATTATTTATTTAAAAGTTATTTTAAACTTATTTGAATTGATTTTGGTGGAACAGATACCATGGAAAGGGTCGTAGGTTCGTGTTAACGAGTGGGAGAGATCCGGCGCTGGTGAACAAAGCTTGTGATTTTCTTAAAGATCATTACTTGGTCCCACCAAATTGGAGGCAAGACGAAAACAAAGGTATGATAAGGAATAAGGATGGTCGTTGGATACAACCCGATAGATCAAAATTTGACGATCATCCAGAAGATATACATCATCATCTCAAACAATTAGGCTTAACTTTTGACTCCTAAATCTTATTTTGTTCATTCAAAATTCATATGAAATCAGAAGCCATTTAGAACGTTGTTTCCTTCTTCTTCATTGATATTTGAACAATAGAAGCTTCTAAGATTAGCCACAACGATCGTAATAATAAGATGACAAGTTTGGTTTACAGAGATAAGCCTGATTCTTCGTCTTCTTCGAGAGTAGAAGTGTAAACGATATAGAATGTCCAAATGAAACCAAAAACTCCCAACAGAATCCAACCAAGAAGATTGTTGCTCAAACCAA

mRNA sequence

ATGGCGATTCACCTCTCCAATGTCCTCTTCTTCCCAATCACAAACTCACTCCACCAATCCAAACAATCATCTTCTTCACTTCATTTCTCAACCTCAAATCGTCACTTTCGTGGTTTCAGATTATTCAGTACAAGGAGAACACAACGACGACGACAATCGACATACGAAGTAGCTTCAAGAACCAGAGACACATCCTCCATTATTACAGACCTCGAAAAACAAACAGAGGGAGATGAACATACCGAAAACGAATCAGTGGCAGCAGAAATCGCTAATTCCTGGCGTGAAATCCATGGCAGCAACGACTGGACCGGGTTGCTTGATCCGATGAACGATCTCCTCCGCTCCGAGATAATCCGCTACGGTGAAATGGCGCAATCCTGCTACGACGCTTTTGATTACGATCCGTTCTCGAAATACTGCGGGAGTTGTCGATTTAGTCGAAGCAAATTCTTCGAACGACTCGGAATGGAAAGCGGAGGTTACGAAGTAACGCGGTACCTCTACGCAACATCAAACATCAACCTACCGAATTTCTTCAAGAAATCGCGATGGCCAAAAGTATGGAGCAAGAACGCGAACTGGATCGGCTACGTCGCGGTATCAAACGACGCGCAATCGAAACTCCTCGGCCGGCGAGACATCGCCATAGCGTGGAGAGGCACCGTAACGCGGTTGGAATGGATCGTGGATCTAATGGATTTTCTGAAACCAGTAGCGGCGGTGAAGATTCCATGCCCTAATTTGGCGGTGAAGGCGGAATCGGGATTTCTGGCTCTGTATACAGACAAGGACGAAGGTTGTGGATACAGCAAGTTGTCAGCGAGAGAGCAGATTGTGACGGAGGTGAAGCGGTTGGTGGAGAGATTCGCCGGAGAAGAGATGAGCATTACGATCACTGGACACAGTTTGGGAAGCGCTCTGGCGGTGTTGAGCGGCTTCGACGTTGCGGAAATGGGTGTGAACCGGTTGGAGAACGGTCGGGTTGTACCGGTTTCGGTTTTCTCATTCTCGGGGCCCCGAGTTGGGAACTTCTCCTTCAAGGAACGCCTCCAAGAATTGGGAGTTAAGGTATTGAGAGTGGTGAATGTCCATGACGTGGTACCCAAGACGCCAGGATTTTTATTCAACGAAAGCGTACCAACGGCTGTGATGAAATTTGCGGAGGAATTACCGTGGAGTTATTCACACGTGGGCGTGGAGTTAAAATTGGATCATAAAATTTCACCGTTCCTCAAGGATACAAACGACCCCGTTTGTGCACATAATTTGGAAGCTCACTTACATTTACTTGATGGATACCATGGAAAGGGTCGTAGGTTCGTGTTAACGAGTGGGAGAGATCCGGCGCTGGTGAACAAAGCTTGTGATTTTCTTAAAGATCATTACTTGGTCCCACCAAATTGGAGGCAAGACGAAAACAAAGGTATGATAAGGAATAAGGATGGTCGTTGGATACAACCCGATAGATCAAAATTTGACGATCATCCAGAAGATATACATCATCATCTCAAACAATTAGGCTTAACTTTTGACTCCTAAATCTTATTTTGTTCATTCAAAATTCATATGAAATCAGAAGCCATTTAGAACGTTGTTTCCTTCTTCTTCATTGATATTTGAACAATAGAAGCTTCTAAGATTAGCCACAACGATCGTAATAATAAGATGACAAGTTTGGTTTACAGAGATAAGCCTGATTCTTCGTCTTCTTCGAGAGTAGAAGTGTAAACGATATAGAATGTCCAAATGAAACCAAAAACTCCCAACAGAATCCAACCAAGAAGATTGTTGCTCAAACCAA

Coding sequence (CDS)

ATGGCGATTCACCTCTCCAATGTCCTCTTCTTCCCAATCACAAACTCACTCCACCAATCCAAACAATCATCTTCTTCACTTCATTTCTCAACCTCAAATCGTCACTTTCGTGGTTTCAGATTATTCAGTACAAGGAGAACACAACGACGACGACAATCGACATACGAAGTAGCTTCAAGAACCAGAGACACATCCTCCATTATTACAGACCTCGAAAAACAAACAGAGGGAGATGAACATACCGAAAACGAATCAGTGGCAGCAGAAATCGCTAATTCCTGGCGTGAAATCCATGGCAGCAACGACTGGACCGGGTTGCTTGATCCGATGAACGATCTCCTCCGCTCCGAGATAATCCGCTACGGTGAAATGGCGCAATCCTGCTACGACGCTTTTGATTACGATCCGTTCTCGAAATACTGCGGGAGTTGTCGATTTAGTCGAAGCAAATTCTTCGAACGACTCGGAATGGAAAGCGGAGGTTACGAAGTAACGCGGTACCTCTACGCAACATCAAACATCAACCTACCGAATTTCTTCAAGAAATCGCGATGGCCAAAAGTATGGAGCAAGAACGCGAACTGGATCGGCTACGTCGCGGTATCAAACGACGCGCAATCGAAACTCCTCGGCCGGCGAGACATCGCCATAGCGTGGAGAGGCACCGTAACGCGGTTGGAATGGATCGTGGATCTAATGGATTTTCTGAAACCAGTAGCGGCGGTGAAGATTCCATGCCCTAATTTGGCGGTGAAGGCGGAATCGGGATTTCTGGCTCTGTATACAGACAAGGACGAAGGTTGTGGATACAGCAAGTTGTCAGCGAGAGAGCAGATTGTGACGGAGGTGAAGCGGTTGGTGGAGAGATTCGCCGGAGAAGAGATGAGCATTACGATCACTGGACACAGTTTGGGAAGCGCTCTGGCGGTGTTGAGCGGCTTCGACGTTGCGGAAATGGGTGTGAACCGGTTGGAGAACGGTCGGGTTGTACCGGTTTCGGTTTTCTCATTCTCGGGGCCCCGAGTTGGGAACTTCTCCTTCAAGGAACGCCTCCAAGAATTGGGAGTTAAGGTATTGAGAGTGGTGAATGTCCATGACGTGGTACCCAAGACGCCAGGATTTTTATTCAACGAAAGCGTACCAACGGCTGTGATGAAATTTGCGGAGGAATTACCGTGGAGTTATTCACACGTGGGCGTGGAGTTAAAATTGGATCATAAAATTTCACCGTTCCTCAAGGATACAAACGACCCCGTTTGTGCACATAATTTGGAAGCTCACTTACATTTACTTGATGGATACCATGGAAAGGGTCGTAGGTTCGTGTTAACGAGTGGGAGAGATCCGGCGCTGGTGAACAAAGCTTGTGATTTTCTTAAAGATCATTACTTGGTCCCACCAAATTGGAGGCAAGACGAAAACAAAGGTATGATAAGGAATAAGGATGGTCGTTGGATACAACCCGATAGATCAAAATTTGACGATCATCCAGAAGATATACATCATCATCTCAAACAATTAGGCTTAACTTTTGACTCCTAA

Protein sequence

MAIHLSNVLFFPITNSLHQSKQSSSSLHFSTSNRHFRGFRLFSTRRTQRRRQSTYEVASRTRDTSSIITDLEKQTEGDEHTENESVAAEIANSWREIHGSNDWTGLLDPMNDLLRSEIIRYGEMAQSCYDAFDYDPFSKYCGSCRFSRSKFFERLGMESGGYEVTRYLYATSNINLPNFFKKSRWPKVWSKNANWIGYVAVSNDAQSKLLGRRDIAIAWRGTVTRLEWIVDLMDFLKPVAAVKIPCPNLAVKAESGFLALYTDKDEGCGYSKLSAREQIVTEVKRLVERFAGEEMSITITGHSLGSALAVLSGFDVAEMGVNRLENGRVVPVSVFSFSGPRVGNFSFKERLQELGVKVLRVVNVHDVVPKTPGFLFNESVPTAVMKFAEELPWSYSHVGVELKLDHKISPFLKDTNDPVCAHNLEAHLHLLDGYHGKGRRFVLTSGRDPALVNKACDFLKDHYLVPPNWRQDENKGMIRNKDGRWIQPDRSKFDDHPEDIHHHLKQLGLTFDS
BLAST of Carg25777-RA vs. NCBI nr
Match: XP_022959614.1 (phospholipase A1-Igamma1, chloroplastic [Cucurbita moschata])

HSP 1 Score: 1047.0 bits (2706), Expect = 2.1e-302
Identity = 510/513 (99.42%), Postives = 511/513 (99.61%), Query Frame = 0

Query: 1   MAIHLSNVLFFPITNSLHQSKQSSSSLHFSTSNRHFRGFRLFSTRRTQRRRQSTYEVASR 60
           MAIHLSNVLFFPITNSLHQSKQSSSSLHFSTSNRHFRGFRLFSTRRTQRRRQSTYEVASR
Sbjct: 1   MAIHLSNVLFFPITNSLHQSKQSSSSLHFSTSNRHFRGFRLFSTRRTQRRRQSTYEVASR 60

Query: 61  TRDTSSIITDLEKQTEGDEHTENESVAAEIANSWREIHGSNDWTGLLDPMNDLLRSEIIR 120
           TRDTSSIITDLEKQTEGDEHTENESVAAEIANSWREIHGSNDWTGLLDPMNDLLRSEIIR
Sbjct: 61  TRDTSSIITDLEKQTEGDEHTENESVAAEIANSWREIHGSNDWTGLLDPMNDLLRSEIIR 120

Query: 121 YGEMAQSCYDAFDYDPFSKYCGSCRFSRSKFFERLGMESGGYEVTRYLYATSNINLPNFF 180
           YGEMAQSCYDAFDYDPFSKYCGSCRFSRSKFFERLGMESGGYEVTRYLYATSNINLPNFF
Sbjct: 121 YGEMAQSCYDAFDYDPFSKYCGSCRFSRSKFFERLGMESGGYEVTRYLYATSNINLPNFF 180

Query: 181 KKSRWPKVWSKNANWIGYVAVSNDAQSKLLGRRDIAIAWRGTVTRLEWIVDLMDFLKPVA 240
           KKSRWPKVWSKNANWIGYVAVSND +SKLLGRRDIAIAWRGTVTRLEWIVDLMDFLKPVA
Sbjct: 181 KKSRWPKVWSKNANWIGYVAVSNDGESKLLGRRDIAIAWRGTVTRLEWIVDLMDFLKPVA 240

Query: 241 AVKIPCPNLAVKAESGFLALYTDKDEGCGYSKLSAREQIVTEVKRLVERFAGEEMSITIT 300
           A KIPCPNLAVKAESGFLALYTDKDEGCGYSKLSAREQIVTEVKRLVERFAGEEMSITIT
Sbjct: 241 AAKIPCPNLAVKAESGFLALYTDKDEGCGYSKLSAREQIVTEVKRLVERFAGEEMSITIT 300

Query: 301 GHSLGSALAVLSGFDVAEMGVNRLENGRVVPVSVFSFSGPRVGNFSFKERLQELGVKVLR 360
           GHSLGSALAVLSGFDVAEMGVNRLENGRVVPVSVFSFSGPRVGNFSFKERLQELGVKVLR
Sbjct: 301 GHSLGSALAVLSGFDVAEMGVNRLENGRVVPVSVFSFSGPRVGNFSFKERLQELGVKVLR 360

Query: 361 VVNVHDVVPKTPGFLFNESVPTAVMKFAEELPWSYSHVGVELKLDHKISPFLKDTNDPVC 420
           VVNVHDVVPKTPGFLFNESVPTAVMKFAEELPWSYSHVGVELKLDHKISPFLKDTNDPVC
Sbjct: 361 VVNVHDVVPKTPGFLFNESVPTAVMKFAEELPWSYSHVGVELKLDHKISPFLKDTNDPVC 420

Query: 421 AHNLEAHLHLLDGYHGKGRRFVLTSGRDPALVNKACDFLKDHYLVPPNWRQDENKGMIRN 480
           AHNLEAHLHLLDGYHGKGRRFVLTSGRDPALVNKACDFLKDHYLVPPNWRQDENKGMIRN
Sbjct: 421 AHNLEAHLHLLDGYHGKGRRFVLTSGRDPALVNKACDFLKDHYLVPPNWRQDENKGMIRN 480

Query: 481 KDGRWIQPDRSKFDDHPEDIHHHLKQLGLTFDS 514
           KDGRWIQPDRSKFDDHPEDIHHHLKQLGLTFDS
Sbjct: 481 KDGRWIQPDRSKFDDHPEDIHHHLKQLGLTFDS 513

BLAST of Carg25777-RA vs. NCBI nr
Match: XP_023514756.1 (phospholipase A1-Igamma1, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1035.8 bits (2677), Expect = 4.9e-299
Identity = 507/514 (98.64%), Postives = 509/514 (99.03%), Query Frame = 0

Query: 1   MAIHLSNVLFFPITNSLHQSKQSSSSLHFSTSNRHFRGFRLFSTRRTQ-RRRQSTYEVAS 60
           MAIHLSNVLFFPITNSLHQSKQSSSSLHFSTSNRHF GFRLFSTRRTQ RRRQSTYEVAS
Sbjct: 26  MAIHLSNVLFFPITNSLHQSKQSSSSLHFSTSNRHFHGFRLFSTRRTQRRRRQSTYEVAS 85

Query: 61  RTRDTSSIITDLEKQTEGDEHTENESVAAEIANSWREIHGSNDWTGLLDPMNDLLRSEII 120
           R RDTSSIITDLEKQTEGDEHTENESVAAEIANSWREIHGSNDWTGLLDPMNDLLRSEII
Sbjct: 86  RIRDTSSIITDLEKQTEGDEHTENESVAAEIANSWREIHGSNDWTGLLDPMNDLLRSEII 145

Query: 121 RYGEMAQSCYDAFDYDPFSKYCGSCRFSRSKFFERLGMESGGYEVTRYLYATSNINLPNF 180
           RYGEMAQSCYDAFDYDPFSKYCGSCRFSRSKFFERLGMESGGYEVTRYLYATSNINLPNF
Sbjct: 146 RYGEMAQSCYDAFDYDPFSKYCGSCRFSRSKFFERLGMESGGYEVTRYLYATSNINLPNF 205

Query: 181 FKKSRWPKVWSKNANWIGYVAVSNDAQSKLLGRRDIAIAWRGTVTRLEWIVDLMDFLKPV 240
           FKKSRWPKVWSKNANWIGYVAVSNDA+SKLLGRRDIAIAWRGTVTRLEWIVDLMDFLKPV
Sbjct: 206 FKKSRWPKVWSKNANWIGYVAVSNDAKSKLLGRRDIAIAWRGTVTRLEWIVDLMDFLKPV 265

Query: 241 AAVKIPCPNLAVKAESGFLALYTDKDEGCGYSKLSAREQIVTEVKRLVERFAGEEMSITI 300
           AA KIPCPNLAVKAESGFLALYTDKDEGCGYSKLSAREQIVTEVKRLVERFAGEEMSITI
Sbjct: 266 AAAKIPCPNLAVKAESGFLALYTDKDEGCGYSKLSAREQIVTEVKRLVERFAGEEMSITI 325

Query: 301 TGHSLGSALAVLSGFDVAEMGVNRLENGRVVPVSVFSFSGPRVGNFSFKERLQELGVKVL 360
           TGHSLGSALAVLSGFDVAEMGVNRLENGRVVPV+VFSFSGPRVGNFSFKERLQELGVKVL
Sbjct: 326 TGHSLGSALAVLSGFDVAEMGVNRLENGRVVPVAVFSFSGPRVGNFSFKERLQELGVKVL 385

Query: 361 RVVNVHDVVPKTPGFLFNESVPTAVMKFAEELPWSYSHVGVELKLDHKISPFLKDTNDPV 420
           RVVNVHDVVPKTPGFL NESVPTAVMKFAEELPWSYSHVGVELKLDHKISPFLKDTNDPV
Sbjct: 386 RVVNVHDVVPKTPGFLINESVPTAVMKFAEELPWSYSHVGVELKLDHKISPFLKDTNDPV 445

Query: 421 CAHNLEAHLHLLDGYHGKGRRFVLTSGRDPALVNKACDFLKDHYLVPPNWRQDENKGMIR 480
           CAHNLEAHLHLLDGYHGKGRRFVLTSGRDPALVNKACDFLKDHYLVPPNWRQDENKGMIR
Sbjct: 446 CAHNLEAHLHLLDGYHGKGRRFVLTSGRDPALVNKACDFLKDHYLVPPNWRQDENKGMIR 505

Query: 481 NKDGRWIQPDRSKFDDHPEDIHHHLKQLGLTFDS 514
           NKDGRWIQPDRSKFDDHPEDIHHHLKQLGLTFDS
Sbjct: 506 NKDGRWIQPDRSKFDDHPEDIHHHLKQLGLTFDS 539

BLAST of Carg25777-RA vs. NCBI nr
Match: XP_023004255.1 (phospholipase A1-Igamma1, chloroplastic [Cucurbita maxima])

HSP 1 Score: 1030.4 bits (2663), Expect = 2.1e-297
Identity = 501/513 (97.66%), Postives = 509/513 (99.22%), Query Frame = 0

Query: 1   MAIHLSNVLFFPITNSLHQSKQSSSSLHFSTSNRHFRGFRLFSTRRTQRRRQSTYEVASR 60
           MAIHLSNVLFFPITNSLHQSKQSSSSLHFSTSNRHFRGFRLFSTRRTQR+RQS YEVASR
Sbjct: 1   MAIHLSNVLFFPITNSLHQSKQSSSSLHFSTSNRHFRGFRLFSTRRTQRQRQSIYEVASR 60

Query: 61  TRDTSSIITDLEKQTEGDEHTENESVAAEIANSWREIHGSNDWTGLLDPMNDLLRSEIIR 120
           TRDTSSIITDLEKQT+GDEHTE +S AAEIA+SWREIHGSNDWTGLLDPMNDLLRSEIIR
Sbjct: 61  TRDTSSIITDLEKQTKGDEHTETKSAAAEIADSWREIHGSNDWTGLLDPMNDLLRSEIIR 120

Query: 121 YGEMAQSCYDAFDYDPFSKYCGSCRFSRSKFFERLGMESGGYEVTRYLYATSNINLPNFF 180
           YGEMAQSCYDAFDYDPFSKYCGSCRFSR+KFFE+LGMESGGYEVTRYLYATSNINLPNFF
Sbjct: 121 YGEMAQSCYDAFDYDPFSKYCGSCRFSRNKFFEQLGMESGGYEVTRYLYATSNINLPNFF 180

Query: 181 KKSRWPKVWSKNANWIGYVAVSNDAQSKLLGRRDIAIAWRGTVTRLEWIVDLMDFLKPVA 240
           KKSRWPKVWSKNANWIGYVAVSNDA+SKLLGRRDIAIAWRGTVTRLEWIVDLMDFLKPVA
Sbjct: 181 KKSRWPKVWSKNANWIGYVAVSNDAKSKLLGRRDIAIAWRGTVTRLEWIVDLMDFLKPVA 240

Query: 241 AVKIPCPNLAVKAESGFLALYTDKDEGCGYSKLSAREQIVTEVKRLVERFAGEEMSITIT 300
           A KIPCPNLAVKAESGFLALYTDKDEGCGYSKLSAREQIVTEVKRLVERFAGEEMSITIT
Sbjct: 241 AAKIPCPNLAVKAESGFLALYTDKDEGCGYSKLSAREQIVTEVKRLVERFAGEEMSITIT 300

Query: 301 GHSLGSALAVLSGFDVAEMGVNRLENGRVVPVSVFSFSGPRVGNFSFKERLQELGVKVLR 360
           GHSLGSALAVLSGFDVAEMGVNRLENGRVVPVSVFSFSGPRVGNFSFKERLQELGVKVLR
Sbjct: 301 GHSLGSALAVLSGFDVAEMGVNRLENGRVVPVSVFSFSGPRVGNFSFKERLQELGVKVLR 360

Query: 361 VVNVHDVVPKTPGFLFNESVPTAVMKFAEELPWSYSHVGVELKLDHKISPFLKDTNDPVC 420
           VVNVHDVVPKTPGFLFNESVPTAVMKFAEELPWSYSHVGVELKLDHKISPFLKDTNDPVC
Sbjct: 361 VVNVHDVVPKTPGFLFNESVPTAVMKFAEELPWSYSHVGVELKLDHKISPFLKDTNDPVC 420

Query: 421 AHNLEAHLHLLDGYHGKGRRFVLTSGRDPALVNKACDFLKDHYLVPPNWRQDENKGMIRN 480
           AHNLEAHLHLLDGYHGKGRRFVLTSGRDPALVNKACDFLKDHYLVPPNWRQDENKGMIRN
Sbjct: 421 AHNLEAHLHLLDGYHGKGRRFVLTSGRDPALVNKACDFLKDHYLVPPNWRQDENKGMIRN 480

Query: 481 KDGRWIQPDRSKFDDHPEDIHHHLKQLGLTFDS 514
           KDGRWIQPDRSKFDDHPEDIH+HLKQLGLTFDS
Sbjct: 481 KDGRWIQPDRSKFDDHPEDIHYHLKQLGLTFDS 513

BLAST of Carg25777-RA vs. NCBI nr
Match: XP_004146952.1 (PREDICTED: phospholipase A1-Igamma1, chloroplastic [Cucumis sativus] >KGN44758.1 hypothetical protein Csa_7G378470 [Cucumis sativus])

HSP 1 Score: 797.3 bits (2058), Expect = 3.0e-227
Identity = 397/517 (76.79%), Postives = 436/517 (84.33%), Query Frame = 0

Query: 1   MAIHLSNVLF-FPITNSLHQSKQSSSSLHFSTSNRHFRGFRLFSTRRTQRRRQSTYEVAS 60
           MAI LSN LF FP        KQ SS L +STSN  F+  RLF TR  ++  Q   +V S
Sbjct: 1   MAILLSNFLFPFP--------KQFSSPLPYSTSNSWFQTSRLFKTRNAEQSSQC--QVIS 60

Query: 61  RTRDTSSIITDLEKQTEGDEHTENESVAAEIANSWREIHGSNDWTGLLDPMNDLLRSEII 120
           RTR + + I + E+Q +   + E     ++IA+SWREIHGSNDWTGLLDPMNDLLRSE+I
Sbjct: 61  RTRHSYASIEEHEQQQQ--INMEESLPESKIADSWREIHGSNDWTGLLDPMNDLLRSELI 120

Query: 121 RYGEMAQSCYDAFDYDPFSKYCGSCRFSRSKFFERLGMESGGYEVTRYLYATSNINLPNF 180
           RYGEM+QSCYDAFDYDPFSKYCGSCRFSR KFFERLGME+ GYEVTRYLYATSNIN+PNF
Sbjct: 121 RYGEMSQSCYDAFDYDPFSKYCGSCRFSRGKFFERLGMENVGYEVTRYLYATSNINMPNF 180

Query: 181 FKKSRWPKVWSKNANWIGYVAVSNDAQSKLLGRRDIAIAWRGTVTRLEWIVDLMDFLKPV 240
           FKKSRWPKVWSK+ANWIGYVAVSND +SK LGRRDI +AWRGTVTRLEWI DLMDFLKP+
Sbjct: 181 FKKSRWPKVWSKSANWIGYVAVSNDEKSKELGRRDIVVAWRGTVTRLEWITDLMDFLKPI 240

Query: 241 AAVKIPCPNLAVKAESGFLALYTDK-DEGCGYSKLSAREQIVTEVKRLVERFAG--EEMS 300
           AA KI CPNL VK ESGF+ LYT+K +EGCGY + SAREQ++ EVKRL ERF G  EEMS
Sbjct: 241 AAAKIGCPNLGVKVESGFVDLYTEKEEEGCGYCRFSAREQVMAEVKRLTERFGGAEEEMS 300

Query: 301 ITITGHSLGSALAVLSGFDVAEMGVNRLENGRVVPVSVFSFSGPRVGNFSFKERLQELGV 360
           ITITGHSLGSALAVLS FD+AE G+NRL NGRVVPV VFSFSGPRVGNFSFKERL ELGV
Sbjct: 301 ITITGHSLGSALAVLSAFDLAETGLNRLGNGRVVPVCVFSFSGPRVGNFSFKERLHELGV 360

Query: 361 KVLRVVNVHDVVPKTPGFLFNESVPTAVMKFAEELPWSYSHVGVELKLDHKISPFLKDTN 420
           KVLRV+N+HD+VPK+PGFL NES+P AVM++AE LPWSYSHVGVELKLDHK+SPFLK TN
Sbjct: 361 KVLRVINIHDIVPKSPGFLLNESIPRAVMQYAEGLPWSYSHVGVELKLDHKVSPFLKQTN 420

Query: 421 DPVCAHNLEAHLHLLDGYHGKGRRFVLTSGRDPALVNKACDFLKDHYLVPPNWRQDENKG 480
           DPVCAHNLEA LHLLDGYH K  RFVL SGRDPALVNK CDFLKDHYLVPPNWRQDENKG
Sbjct: 421 DPVCAHNLEALLHLLDGYHEKDGRFVLASGRDPALVNKGCDFLKDHYLVPPNWRQDENKG 480

Query: 481 MIRNKDGRWIQPDRSKFDDHPEDIHHHLKQLGLTFDS 514
           MIRNKDGRWIQPDR KF+DHP DIHHHL QLGL F S
Sbjct: 481 MIRNKDGRWIQPDRLKFEDHPHDIHHHLTQLGLHFYS 505

BLAST of Carg25777-RA vs. NCBI nr
Match: XP_008451275.1 (PREDICTED: phospholipase A1-Igamma1, chloroplastic-like isoform X1 [Cucumis melo])

HSP 1 Score: 777.7 bits (2007), Expect = 2.4e-221
Identity = 386/517 (74.66%), Postives = 420/517 (81.24%), Query Frame = 0

Query: 1   MAIHLSNVLF-FPITNSLHQSKQSSSSLHFSTSNRHFRGFRLFSTRRTQRRRQSTYEVAS 60
           MAI LSN LF FP    LH                            T++  Q   +V S
Sbjct: 1   MAILLSNFLFLFP--KQLHHXXXXXXXXXXXXXXXXXXXXXXXXXXNTEQSSQC--QVIS 60

Query: 61  RTRDTSSIITDLEKQTEGDEHTENESVAAEIANSWREIHGSNDWTGLLDPMNDLLRSEII 120
           RTRD+ +   +         + E     ++IA+SWREIHGSNDWTGLLDPMNDLLRSE+I
Sbjct: 61  RTRDSYAFTEEHXXXXXXXINMEESLPESKIADSWREIHGSNDWTGLLDPMNDLLRSELI 120

Query: 121 RYGEMAQSCYDAFDYDPFSKYCGSCRFSRSKFFERLGMESGGYEVTRYLYATSNINLPNF 180
           RYGEM+QSCYDAFDYDPFSKYCGSCRFSR KFFERLGME+ GYEVTRYLYATSNIN+PNF
Sbjct: 121 RYGEMSQSCYDAFDYDPFSKYCGSCRFSRGKFFERLGMENVGYEVTRYLYATSNINMPNF 180

Query: 181 FKKSRWPKVWSKNANWIGYVAVSNDAQSKLLGRRDIAIAWRGTVTRLEWIVDLMDFLKPV 240
           FKKSRWPKVWSK+ANWIGYVAVSND +SK LGRRDI +AWRGTVTRLEWI DLMDFLKP+
Sbjct: 181 FKKSRWPKVWSKSANWIGYVAVSNDEKSKELGRRDIVVAWRGTVTRLEWITDLMDFLKPI 240

Query: 241 AAVKIPCPNLAVKAESGFLALYTDKDE-GCGYSKLSAREQIVTEVKRLVERFAG--EEMS 300
           A  KI CPN  VK ESGF+ LYT+K+E GCGY + SAREQ++ EVKRL ERF G  EEMS
Sbjct: 241 AEAKIGCPNSGVKVESGFVDLYTEKEEKGCGYCRFSAREQVMAEVKRLTERFGGAEEEMS 300

Query: 301 ITITGHSLGSALAVLSGFDVAEMGVNRLENGRVVPVSVFSFSGPRVGNFSFKERLQELGV 360
           ITITGHSLGSALAVLSGFD+AE G+NRL NGR+VPV VFSFSGPRVGNFSFKE L ELGV
Sbjct: 301 ITITGHSLGSALAVLSGFDIAETGLNRLGNGRLVPVCVFSFSGPRVGNFSFKEHLHELGV 360

Query: 361 KVLRVVNVHDVVPKTPGFLFNESVPTAVMKFAEELPWSYSHVGVELKLDHKISPFLKDTN 420
           KVLRVVN+HD+VPKTPGFLFNES+P AVM+FAE LPWSYSHVGVELKLDHK+SPFLK TN
Sbjct: 361 KVLRVVNIHDIVPKTPGFLFNESIPRAVMQFAEGLPWSYSHVGVELKLDHKVSPFLKQTN 420

Query: 421 DPVCAHNLEAHLHLLDGYHGKGRRFVLTSGRDPALVNKACDFLKDHYLVPPNWRQDENKG 480
           DPVCAHNLEA LHLLDGYHGKG RFVL SGRDPALVNK CDFLKDHYLVPPNWRQDENKG
Sbjct: 421 DPVCAHNLEALLHLLDGYHGKGGRFVLASGRDPALVNKGCDFLKDHYLVPPNWRQDENKG 480

Query: 481 MIRNKDGRWIQPDRSKFDDHPEDIHHHLKQLGLTFDS 514
           MIRN+DGRWIQPDR KFDDHP+DIHHHL QLGL F S
Sbjct: 481 MIRNQDGRWIQPDRLKFDDHPQDIHHHLTQLGLHFYS 513

BLAST of Carg25777-RA vs. TAIR10
Match: AT1G06800.1 (alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 632.9 bits (1631), Expect = 1.8e-181
Identity = 316/478 (66.11%), Postives = 367/478 (76.78%), Query Frame = 0

Query: 43  STRRTQRRRQSTYEVASRTRDTSSIITDLEKQTEG----DEHTENESVAAE---IANSWR 102
           ST  T   R S     SRT + +SI T LE+++ G    ++    +  A E   + ++WR
Sbjct: 31  STLITFPARSSPARAMSRTDEEASISTRLEQESYGLTTAEDIRRRDGEAKESKRLRDTWR 90

Query: 103 EIHGSNDWTGLLDPMNDLLRSEIIRYGEMAQSCYDAFDYDPFSKYCGSCRFSRSKFFERL 162
           +I G +DW GL+DPM+ +LRSE+IRYGEMAQ+CYDAFD+DPFS+YCGSCRF+R   F+ L
Sbjct: 91  KIQGEDDWAGLMDPMDPVLRSELIRYGEMAQACYDAFDFDPFSRYCGSCRFTRRHLFDSL 150

Query: 163 GMESGGYEVTRYLYATSNINLPNFFKKSRWPKVWSKNANWIGYVAVS--NDAQSKLLGRR 222
           G+   GYEV RYLYATSNINLPNFF KSRW KVWSKNANW+GYVAVS  N+A    LGRR
Sbjct: 151 GIIDSGYEVARYLYATSNINLPNFFSKSRWSKVWSKNANWMGYVAVSDDNEATRCRLGRR 210

Query: 223 DIAIAWRGTVTRLEWIVDLMDFLKPVAAVKIPCPNLAVKAESGFLALYTDKDEGCGYSKL 282
           DIAIAWRGTVTRLEWI DL DFLKPV+     CP+ AVKAESGFL LYTDKD  C +SK 
Sbjct: 211 DIAIAWRGTVTRLEWIADLKDFLKPVSGNGFRCPDPAVKAESGFLDLYTDKDTSCNFSKF 270

Query: 283 SAREQIVTEVKRLVERFA---GEEMSITITGHSLGSALAVLSGFDVAEMGVNRLENGRVV 342
           SAREQ++TEVKRLVER+    GEE+SIT+TGHSLG ALAVLS +DVAEMGVNR   G+V+
Sbjct: 271 SAREQVLTEVKRLVERYGDEEGEELSITVTGHSLGGALAVLSAYDVAEMGVNRTRKGKVI 330

Query: 343 PVSVFSFSGPRVGNFSFKERLQELGVKVLRVVNVHDVVPKTPGFLFNESVPTAVMKFAEE 402
           PV+ F++ GPRVGN  FKER+++LGVKVLRVVN HDVV K+PG   NE  P A+MK A  
Sbjct: 331 PVTAFTYGGPRVGNIRFKERIEKLGVKVLRVVNEHDVVAKSPGLFLNERAPQALMKLAGG 390

Query: 403 LPWSYSHVGVELKLDHKISPFLKDTNDPVCAHNLEAHLHLLDGYHGKGRRFVLTSGRDPA 462
           LPW YSHVG  L LDH+ SPFLK T D   AHNLEA LHLLDGYHGKG+RFVL+SGRDPA
Sbjct: 391 LPWCYSHVGEMLPLDHQKSPFLKPTVDLSTAHNLEALLHLLDGYHGKGQRFVLSSGRDPA 450

Query: 463 LVNKACDFLKDHYLVPPNWRQDENKGMIRNKDGRWIQPDRSKFDD-HPEDIHHHLKQL 508
           LVNKA DFLKDH++VPP WRQD NKGM+RN DGRWIQPDR + DD H  DIH  L QL
Sbjct: 451 LVNKASDFLKDHFMVPPYWRQDANKGMVRNTDGRWIQPDRIRADDQHAPDIHQLLTQL 508

BLAST of Carg25777-RA vs. TAIR10
Match: AT2G30550.2 (alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 611.7 bits (1576), Expect = 4.2e-175
Identity = 298/471 (63.27%), Postives = 359/471 (76.22%), Query Frame = 0

Query: 44  TRRTQRRRQSTYEVASRTRDTSSIITDLEKQTEGDEHTENESVAAEIANSWREIHGSNDW 103
           +R  + RR+    +         +    E     D+ TE E     + ++WR+I G +DW
Sbjct: 58  SRLERERRERQGLLIEEAEGAGELWMTAEDIRRRDKKTEEE---RRLRDTWRKIQGEDDW 117

Query: 104 TGLLDPMNDLLRSEIIRYGEMAQSCYDAFDYDPFSKYCGSCRFSRSKFFERLGMESGGYE 163
            GL+DPM+ +LRSE+IRYGEMAQ+CYDAFD+DP SKYCG+ RF+R +FF+ LGM   GYE
Sbjct: 118 AGLMDPMDPILRSELIRYGEMAQACYDAFDFDPASKYCGTSRFTRLEFFDSLGMIDSGYE 177

Query: 164 VTRYLYATSNINLPNFFKKSRWPKVWSKNANWIGYVAVSNDAQSK-LLGRRDIAIAWRGT 223
           V RYLYATSNINLPNFF KSRW KVWSKNANW+GYVAVS+D  S+  LGRRDIAIAWRGT
Sbjct: 178 VARYLYATSNINLPNFFSKSRWSKVWSKNANWMGYVAVSDDETSRNRLGRRDIAIAWRGT 237

Query: 224 VTRLEWIVDLMDFLKPVAAVKIPCPNLAVKAESGFLALYTDKDEGCGYSKLSAREQIVTE 283
           VT+LEWI DL D+LKPV   KI CP+ AVK ESGFL LYTDKD  C +++ SAREQI+TE
Sbjct: 238 VTKLEWIADLKDYLKPVTENKIRCPDPAVKVESGFLDLYTDKDTTCKFARFSAREQILTE 297

Query: 284 VKRLVERFAGE---EMSITITGHSLGSALAVLSGFDVAEMGVNRLENGRVVPVSVFSFSG 343
           VKRLVE    +   ++SIT+TGHSLG ALA+LS +D+AEM +NR + G+V+PV+V ++ G
Sbjct: 298 VKRLVEEHGDDDDSDLSITVTGHSLGGALAILSAYDIAEMRLNRSKKGKVIPVTVLTYGG 357

Query: 344 PRVGNFSFKERLQELGVKVLRVVNVHDVVPKTPGFLFNESVPTAVMKFAEELPWSYSHVG 403
           PRVGN  F+ER++ELGVKV+RVVNVHDVVPK+PG   NES P A+MK AE LPW YSHVG
Sbjct: 358 PRVGNVRFRERMEELGVKVMRVVNVHDVVPKSPGLFLNESRPHALMKIAEGLPWCYSHVG 417

Query: 404 VELKLDHKISPFLKDTNDPVCAHNLEAHLHLLDGYHGKGRRFVLTSGRDPALVNKACDFL 463
            EL LDH+ SPFLK + D   AHNLEA LHLLDGYHGKG RFVL+SGRD ALVNKA DFL
Sbjct: 418 EELALDHQNSPFLKPSVDVSTAHNLEAMLHLLDGYHGKGERFVLSSGRDHALVNKASDFL 477

Query: 464 KDHYLVPPNWRQDENKGMIRNKDGRWIQPDRSKFDD-HPEDIHHHLKQLGL 510
           K+H  +PP WRQD NKGM+RN +GRWIQ +R +F+D H  DIHHHL QL L
Sbjct: 478 KEHLQIPPFWRQDANKGMVRNSEGRWIQAERLRFEDHHSPDIHHHLSQLRL 525

BLAST of Carg25777-RA vs. TAIR10
Match: AT1G51440.1 (alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 511.1 bits (1315), Expect = 7.7e-145
Identity = 254/429 (59.21%), Postives = 309/429 (72.03%), Query Frame = 0

Query: 94  WREIHGSNDWTGLLDPMNDLLRSEIIRYGEMAQSCYDAFDYDPFSKYCGSCRFSRSKFFE 153
           WRE+ G N+W G LDPMN+ LR EIIRYGE AQ+CYD+FD+DP SKYCGSC++  S FF 
Sbjct: 91  WREVQGCNNWEGQLDPMNNHLRREIIRYGEFAQACYDSFDFDPHSKYCGSCKYHPSDFFL 150

Query: 154 RLGME-SGGYEVTRYLYATSNINLPNFFKKSRWPKVWSKNANWIGYVAVSNDAQS-KLLG 213
            L +    GY +TRYLYATSNINLPNFF+KS+   +WS++ANW+G+VAV+ D +    LG
Sbjct: 151 NLDLHLHKGYTITRYLYATSNINLPNFFQKSKLSSIWSQHANWMGFVAVATDEEEVSRLG 210

Query: 214 RRDIAIAWRGTVTRLEWIVDLMDFLKPVAAVKIPCPNLAVKAESGFLALYTDKDEGCGYS 273
           RRDI IAWRGTVT LEWI DL D L        P    ++K E GF  LYT K++ C +S
Sbjct: 211 RRDIVIAWRGTVTYLEWIYDLKDILCSANFGDDP----SIKIELGFHDLYTKKEDSCKFS 270

Query: 274 KLSAREQIVTEVKRLVERFAGEE----MSITITGHSLGSALAVLSGFDVAEMGVNRL-EN 333
             SAREQ++ EVKRL+E +  EE     SIT+TGHSLG++LA++S +D+AE+ +N + EN
Sbjct: 271 SFSAREQVLAEVKRLIEYYGTEEEGHKTSITVTGHSLGASLALVSAYDIAELNLNHVPEN 330

Query: 334 GRVVPVSVFSFSGPRVGNFSFKERLQELGVKVLRVVNVHDVVPKTPGFLFNESVPTAVMK 393
              +P++VFSFSGPRVGN  FKER  ELGVKVLRVVNVHD VP  PG   NE       K
Sbjct: 331 NYKIPITVFSFSGPRVGNLRFKERCDELGVKVLRVVNVHDKVPSVPGIFTNEKF--QFQK 390

Query: 394 FAEE---LPWSYSHVGVELKLDHKISPFLKDTNDPVCAHNLEAHLHLLDGYHGKG----R 453
           + EE    PWSY+HVGVEL LDHK SPFLK T D  CAHNLEA LHL+DGYHGK     +
Sbjct: 391 YVEEKTSFPWSYAHVGVELALDHKKSPFLKPTKDLGCAHNLEALLHLVDGYHGKDEEAEK 450

Query: 454 RFVLTSGRDPALVNKACDFLKDHYLVPPNWRQDENKGMIRNKDGRWIQPDRSKFDDH-PE 508
           RF L + RD ALVNK+CDFL+  Y VPP WRQDENKGM++N DG+W+ PDR   + H PE
Sbjct: 451 RFCLVTKRDIALVNKSCDFLRGEYHVPPCWRQDENKGMVKNGDGQWVLPDRPLLEPHGPE 510

BLAST of Carg25777-RA vs. TAIR10
Match: AT1G30370.1 (alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 393.7 bits (1010), Expect = 1.8e-109
Identity = 215/484 (44.42%), Postives = 298/484 (61.57%), Query Frame = 0

Query: 29  FSTSNRHFRGFRLFSTRRTQRRRQSTYEVASRTRDTS---SIITDLEKQT------EGDE 88
           F ++ +H   F + S +  Q   Q    +   + D++   + +  L K +       GD 
Sbjct: 46  FLSTKKHL--FHIKSRKEEQDLSQVAQRICKISNDSTKSLAFLLQLPKYSADDFLDRGDL 105

Query: 89  HTENESVAAEIANSWREIHGSNDWTGLLDPMNDLLRSEIIRYGEMAQSCYDAFDYDPFSK 148
            T   S   +I+  WRE+HGSN+W  LLDP++  LR E+ +YGE  +S YD+ D+DP S+
Sbjct: 106 MTPAASPREKISKMWRELHGSNNWENLLDPLHPWLRREVTKYGEFVESVYDSLDFDPLSE 165

Query: 149 YCGSCRFSRSKFFERLGMESGGYEVTRYLYATSNINLPNFFKKSRWPKVWSKNANWIGYV 208
           +CGS R++R+K FE LG+   GY+VT+Y+YA S +++P +F  S   + WSK++NW+G+V
Sbjct: 166 FCGSSRYNRNKLFEELGLTRHGYKVTKYIYAMSRVDVPQWFLSSALGETWSKDSNWMGFV 225

Query: 209 AVSNDAQSKLLGRRDIAIAWRGTVTRLEWIVDLMDFLKPVAAVKIPCPNLAVKAESGFLA 268
           AVS D +S  +GRRDI +AWRGTVT  EW +DL   ++P    +       VK +SGFL+
Sbjct: 226 AVSGDRESLRIGRRDIVVAWRGTVTPTEWFMDLRTSMEPFDC-EGKHGKTVVKVQSGFLS 285

Query: 269 LYTDKDEGCGYSKLSAREQIVTEVKRLVERF--AGEEMSITITGHSLGSALAVLSGFDVA 328
           +Y  K E   Y+K SA EQ + EVKRLV  F   GEE+S+TITGHSLG ALA+++ ++ A
Sbjct: 286 IYNSKSELTRYNKESASEQTMDEVKRLVNFFKDRGEEVSLTITGHSLGGALALMNAYEAA 345

Query: 329 EMGVNRLENGRVVPVSVFSFSGPRVGNFSFKERLQELGVKVLRVVNVHDVVPKTPGFLFN 388
              V  L       +SV SF  PRVGN +FKE+L  LGVKVLRVVN  D+VPK PG +FN
Sbjct: 346 R-DVPALSGN----ISVISFGAPRVGNLAFKEKLNSLGVKVLRVVNKQDIVPKLPGIVFN 405

Query: 389 ESVPTAVMKFAEELPWSYSHVGVELKLDHKISPFLKDTNDPVCAHNLEAHLHLLDGYHGK 448
           + V   +      L W Y HVG +LKLD   SP++K  +D   AHNLE +LH+LDG+H K
Sbjct: 406 K-VLNKLNPITSRLNWVYRHVGTQLKLDVFSSPYVKRDSDLGRAHNLEVYLHVLDGFHRK 465

Query: 449 GRRFVLTSGRDPALVNKACDFLKDHYLVPPNWRQDENKGMIRNKD-GRWIQPDRSKFDDH 501
              F + + RD A VNK+ D L DH  +P  W Q  +KG+I NK  GRW++P R+     
Sbjct: 466 KSGFRVNARRDVASVNKSTDMLLDHLRIPEFWYQVAHKGLILNKQTGRWVKPVRA----- 515

BLAST of Carg25777-RA vs. TAIR10
Match: AT2G31690.1 (alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 307.0 bits (785), Expect = 2.2e-83
Identity = 164/375 (43.73%), Postives = 228/375 (60.80%), Query Frame = 0

Query: 90  IANSWREIHGSNDWTGLLDPMNDLLRSEIIRYGEMAQSCYDAFDYDPFSKYCGSCRFSRS 149
           ++  WREI G N+W  L++P+N LL+ EI RYG +  +CY AFD DP SK   +C++ + 
Sbjct: 88  LSRVWREIQGCNNWKDLIEPLNPLLQQEITRYGNLVSTCYKAFDLDPNSKRYLNCKYGKQ 147

Query: 150 KFFERLGMES-GGYEVTRYLYATSNINLPNFFKKSRWPKVWSKNANWIGYVAVSNDAQSK 209
              +   ++    Y+VT+Y+YAT +IN+      S      ++ A W+GYVA S+D   K
Sbjct: 148 TLLKETEIDQPEDYQVTKYIYATPDINI----NISPIQNEMNRRARWVGYVAASSDDSVK 207

Query: 210 LLGRRDIAIAWRGTVTRLEWIVDLMDFLKPVAAVKIPCPNLAVKAESGFLALYTDKDEGC 269
            LGRRDI + +RGTVT  EW+ + M  L P A      P L VK ESGFL+LYT  +   
Sbjct: 208 RLGRRDIVVTFRGTVTNPEWLANFMSSLTP-ARFHPHNPRLDVKVESGFLSLYTSDESES 267

Query: 270 GYSKLSAREQIVTEVKRLVERFAGEEMSITITGHSLGSALAVLSGFDVAEMGVNRLENGR 329
            +   S R+Q+++E+ RL+ ++ GEEMSIT+ GHS+GS+LA L  +D+AE+G+NR     
Sbjct: 268 KFGLESCRQQLLSEISRLMNKYKGEEMSITLAGHSMGSSLAQLLAYDIAELGLNRRIGKG 327

Query: 330 VVPVSVFSFSGPRVGNFSFKERLQELGVKVLRVVNVHDVVPKTPGFLFNESVPTAVMKFA 389
            +PV+VFSF+GPRVGN  FK+R +ELGVKVLR+ NV+D V K PG LFNE+    V+   
Sbjct: 328 DIPVTVFSFAGPRVGNLEFKKRCEELGVKVLRITNVNDPVTKLPGVLFNENF--RVLGGF 387

Query: 390 EELPWS---YSHVGVELKLDHKISPFLKDTNDPVCAHNLEAHLHLLDGYHGKGRRFVLTS 449
            ELPWS   Y HVGVEL LD        D  +  C H+L+ ++ LL+      R   + S
Sbjct: 388 YELPWSCSCYVHVGVELTLD------FFDVQNISCVHDLQTYIDLLNQRRTNSRS--VDS 447

Query: 450 GRDPALVNKACDFLK 461
                  N A +FLK
Sbjct: 448 XXXXXXXNVALEFLK 447

BLAST of Carg25777-RA vs. Swiss-Prot
Match: sp|Q941F1|PLA15_ARATH (Phospholipase A1-Igamma1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At1g06800 PE=1 SV=2)

HSP 1 Score: 632.9 bits (1631), Expect = 3.2e-180
Identity = 316/478 (66.11%), Postives = 367/478 (76.78%), Query Frame = 0

Query: 43  STRRTQRRRQSTYEVASRTRDTSSIITDLEKQTEG----DEHTENESVAAE---IANSWR 102
           ST  T   R S     SRT + +SI T LE+++ G    ++    +  A E   + ++WR
Sbjct: 31  STLITFPARSSPARAMSRTDEEASISTRLEQESYGLTTAEDIRRRDGEAKESKRLRDTWR 90

Query: 103 EIHGSNDWTGLLDPMNDLLRSEIIRYGEMAQSCYDAFDYDPFSKYCGSCRFSRSKFFERL 162
           +I G +DW GL+DPM+ +LRSE+IRYGEMAQ+CYDAFD+DPFS+YCGSCRF+R   F+ L
Sbjct: 91  KIQGEDDWAGLMDPMDPVLRSELIRYGEMAQACYDAFDFDPFSRYCGSCRFTRRHLFDSL 150

Query: 163 GMESGGYEVTRYLYATSNINLPNFFKKSRWPKVWSKNANWIGYVAVS--NDAQSKLLGRR 222
           G+   GYEV RYLYATSNINLPNFF KSRW KVWSKNANW+GYVAVS  N+A    LGRR
Sbjct: 151 GIIDSGYEVARYLYATSNINLPNFFSKSRWSKVWSKNANWMGYVAVSDDNEATRCRLGRR 210

Query: 223 DIAIAWRGTVTRLEWIVDLMDFLKPVAAVKIPCPNLAVKAESGFLALYTDKDEGCGYSKL 282
           DIAIAWRGTVTRLEWI DL DFLKPV+     CP+ AVKAESGFL LYTDKD  C +SK 
Sbjct: 211 DIAIAWRGTVTRLEWIADLKDFLKPVSGNGFRCPDPAVKAESGFLDLYTDKDTSCNFSKF 270

Query: 283 SAREQIVTEVKRLVERFA---GEEMSITITGHSLGSALAVLSGFDVAEMGVNRLENGRVV 342
           SAREQ++TEVKRLVER+    GEE+SIT+TGHSLG ALAVLS +DVAEMGVNR   G+V+
Sbjct: 271 SAREQVLTEVKRLVERYGDEEGEELSITVTGHSLGGALAVLSAYDVAEMGVNRTRKGKVI 330

Query: 343 PVSVFSFSGPRVGNFSFKERLQELGVKVLRVVNVHDVVPKTPGFLFNESVPTAVMKFAEE 402
           PV+ F++ GPRVGN  FKER+++LGVKVLRVVN HDVV K+PG   NE  P A+MK A  
Sbjct: 331 PVTAFTYGGPRVGNIRFKERIEKLGVKVLRVVNEHDVVAKSPGLFLNERAPQALMKLAGG 390

Query: 403 LPWSYSHVGVELKLDHKISPFLKDTNDPVCAHNLEAHLHLLDGYHGKGRRFVLTSGRDPA 462
           LPW YSHVG  L LDH+ SPFLK T D   AHNLEA LHLLDGYHGKG+RFVL+SGRDPA
Sbjct: 391 LPWCYSHVGEMLPLDHQKSPFLKPTVDLSTAHNLEALLHLLDGYHGKGQRFVLSSGRDPA 450

Query: 463 LVNKACDFLKDHYLVPPNWRQDENKGMIRNKDGRWIQPDRSKFDD-HPEDIHHHLKQL 508
           LVNKA DFLKDH++VPP WRQD NKGM+RN DGRWIQPDR + DD H  DIH  L QL
Sbjct: 451 LVNKASDFLKDHFMVPPYWRQDANKGMVRNTDGRWIQPDRIRADDQHAPDIHQLLTQL 508

BLAST of Carg25777-RA vs. Swiss-Prot
Match: sp|Q3EBR6|PLA16_ARATH (Phospholipase A1-Igamma2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At2g30550 PE=1 SV=2)

HSP 1 Score: 611.7 bits (1576), Expect = 7.5e-174
Identity = 298/471 (63.27%), Postives = 359/471 (76.22%), Query Frame = 0

Query: 44  TRRTQRRRQSTYEVASRTRDTSSIITDLEKQTEGDEHTENESVAAEIANSWREIHGSNDW 103
           +R  + RR+    +         +    E     D+ TE E     + ++WR+I G +DW
Sbjct: 58  SRLERERRERQGLLIEEAEGAGELWMTAEDIRRRDKKTEEE---RRLRDTWRKIQGEDDW 117

Query: 104 TGLLDPMNDLLRSEIIRYGEMAQSCYDAFDYDPFSKYCGSCRFSRSKFFERLGMESGGYE 163
            GL+DPM+ +LRSE+IRYGEMAQ+CYDAFD+DP SKYCG+ RF+R +FF+ LGM   GYE
Sbjct: 118 AGLMDPMDPILRSELIRYGEMAQACYDAFDFDPASKYCGTSRFTRLEFFDSLGMIDSGYE 177

Query: 164 VTRYLYATSNINLPNFFKKSRWPKVWSKNANWIGYVAVSNDAQSK-LLGRRDIAIAWRGT 223
           V RYLYATSNINLPNFF KSRW KVWSKNANW+GYVAVS+D  S+  LGRRDIAIAWRGT
Sbjct: 178 VARYLYATSNINLPNFFSKSRWSKVWSKNANWMGYVAVSDDETSRNRLGRRDIAIAWRGT 237

Query: 224 VTRLEWIVDLMDFLKPVAAVKIPCPNLAVKAESGFLALYTDKDEGCGYSKLSAREQIVTE 283
           VT+LEWI DL D+LKPV   KI CP+ AVK ESGFL LYTDKD  C +++ SAREQI+TE
Sbjct: 238 VTKLEWIADLKDYLKPVTENKIRCPDPAVKVESGFLDLYTDKDTTCKFARFSAREQILTE 297

Query: 284 VKRLVERFAGE---EMSITITGHSLGSALAVLSGFDVAEMGVNRLENGRVVPVSVFSFSG 343
           VKRLVE    +   ++SIT+TGHSLG ALA+LS +D+AEM +NR + G+V+PV+V ++ G
Sbjct: 298 VKRLVEEHGDDDDSDLSITVTGHSLGGALAILSAYDIAEMRLNRSKKGKVIPVTVLTYGG 357

Query: 344 PRVGNFSFKERLQELGVKVLRVVNVHDVVPKTPGFLFNESVPTAVMKFAEELPWSYSHVG 403
           PRVGN  F+ER++ELGVKV+RVVNVHDVVPK+PG   NES P A+MK AE LPW YSHVG
Sbjct: 358 PRVGNVRFRERMEELGVKVMRVVNVHDVVPKSPGLFLNESRPHALMKIAEGLPWCYSHVG 417

Query: 404 VELKLDHKISPFLKDTNDPVCAHNLEAHLHLLDGYHGKGRRFVLTSGRDPALVNKACDFL 463
            EL LDH+ SPFLK + D   AHNLEA LHLLDGYHGKG RFVL+SGRD ALVNKA DFL
Sbjct: 418 EELALDHQNSPFLKPSVDVSTAHNLEAMLHLLDGYHGKGERFVLSSGRDHALVNKASDFL 477

Query: 464 KDHYLVPPNWRQDENKGMIRNKDGRWIQPDRSKFDD-HPEDIHHHLKQLGL 510
           K+H  +PP WRQD NKGM+RN +GRWIQ +R +F+D H  DIHHHL QL L
Sbjct: 478 KEHLQIPPFWRQDANKGMVRNSEGRWIQAERLRFEDHHSPDIHHHLSQLRL 525

BLAST of Carg25777-RA vs. Swiss-Prot
Match: sp|Q9C8J6|PLA17_ARATH (Phospholipase A1-Igamma3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At1g51440 PE=1 SV=1)

HSP 1 Score: 511.1 bits (1315), Expect = 1.4e-143
Identity = 254/429 (59.21%), Postives = 309/429 (72.03%), Query Frame = 0

Query: 94  WREIHGSNDWTGLLDPMNDLLRSEIIRYGEMAQSCYDAFDYDPFSKYCGSCRFSRSKFFE 153
           WRE+ G N+W G LDPMN+ LR EIIRYGE AQ+CYD+FD+DP SKYCGSC++  S FF 
Sbjct: 91  WREVQGCNNWEGQLDPMNNHLRREIIRYGEFAQACYDSFDFDPHSKYCGSCKYHPSDFFL 150

Query: 154 RLGME-SGGYEVTRYLYATSNINLPNFFKKSRWPKVWSKNANWIGYVAVSNDAQS-KLLG 213
            L +    GY +TRYLYATSNINLPNFF+KS+   +WS++ANW+G+VAV+ D +    LG
Sbjct: 151 NLDLHLHKGYTITRYLYATSNINLPNFFQKSKLSSIWSQHANWMGFVAVATDEEEVSRLG 210

Query: 214 RRDIAIAWRGTVTRLEWIVDLMDFLKPVAAVKIPCPNLAVKAESGFLALYTDKDEGCGYS 273
           RRDI IAWRGTVT LEWI DL D L        P    ++K E GF  LYT K++ C +S
Sbjct: 211 RRDIVIAWRGTVTYLEWIYDLKDILCSANFGDDP----SIKIELGFHDLYTKKEDSCKFS 270

Query: 274 KLSAREQIVTEVKRLVERFAGEE----MSITITGHSLGSALAVLSGFDVAEMGVNRL-EN 333
             SAREQ++ EVKRL+E +  EE     SIT+TGHSLG++LA++S +D+AE+ +N + EN
Sbjct: 271 SFSAREQVLAEVKRLIEYYGTEEEGHKTSITVTGHSLGASLALVSAYDIAELNLNHVPEN 330

Query: 334 GRVVPVSVFSFSGPRVGNFSFKERLQELGVKVLRVVNVHDVVPKTPGFLFNESVPTAVMK 393
              +P++VFSFSGPRVGN  FKER  ELGVKVLRVVNVHD VP  PG   NE       K
Sbjct: 331 NYKIPITVFSFSGPRVGNLRFKERCDELGVKVLRVVNVHDKVPSVPGIFTNEKF--QFQK 390

Query: 394 FAEE---LPWSYSHVGVELKLDHKISPFLKDTNDPVCAHNLEAHLHLLDGYHGKG----R 453
           + EE    PWSY+HVGVEL LDHK SPFLK T D  CAHNLEA LHL+DGYHGK     +
Sbjct: 391 YVEEKTSFPWSYAHVGVELALDHKKSPFLKPTKDLGCAHNLEALLHLVDGYHGKDEEAEK 450

Query: 454 RFVLTSGRDPALVNKACDFLKDHYLVPPNWRQDENKGMIRNKDGRWIQPDRSKFDDH-PE 508
           RF L + RD ALVNK+CDFL+  Y VPP WRQDENKGM++N DG+W+ PDR   + H PE
Sbjct: 451 RFCLVTKRDIALVNKSCDFLRGEYHVPPCWRQDENKGMVKNGDGQWVLPDRPLLEPHGPE 510

BLAST of Carg25777-RA vs. Swiss-Prot
Match: sp|Q6F358|PLA6_ORYSJ (Phospholipase A1-II 6 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0574000 PE=2 SV=1)

HSP 1 Score: 313.2 bits (801), Expect = 5.5e-84
Identity = 178/421 (42.28%), Postives = 241/421 (57.24%), Query Frame = 0

Query: 91  ANSWREIHGSNDWTGLLDPMNDLLRSEIIRYGEMAQSCYDAFDYDPFSKYCGSCRFSRSK 150
           A  WRE+HG +DW GLLDP +  LR  +IRYGEMAQ+ YDAF+++  S + G  RF+  +
Sbjct: 12  ARRWRELHGESDWDGLLDPFDLDLRRTVIRYGEMAQATYDAFNHEKLSPHAGLSRFAARR 71

Query: 151 FFER--LGMESGGYEVTRYLYATSNINLPN-FFKKSRWPKVWSKNANWIGYVAVSNDAQS 210
           FFER  L   S  Y V R++YATS + +P     +S       + +NWIGYVAV+ D   
Sbjct: 72  FFERAQLPGHSAAYRVARFVYATSCVAVPEPLILRSASRARRCRESNWIGYVAVATDEGK 131

Query: 211 KLLGRRDIAIAWRGTVTRLEWIVDLMDF--LKPVAAVKIPCPNLAVKAESGFLALYTDKD 270
             LGRRDI +AWRGTV  LEWI D MDF  + P   ++    +  V    G+L++YT +D
Sbjct: 132 AALGRRDIVVAWRGTVQSLEWIKD-MDFVMVPPKGLLRDKASDAMV--HRGWLSMYTSRD 191

Query: 271 EGCGYSKLSAREQIVTEVKRLVERFAGEEMSITITGHSLGSALAVLSGFDVAEMGVNRLE 330
               ++K SAR+Q+++EV +LV  +  EE+SIT+TGHSLG+ALA L+ FD+ E G NR  
Sbjct: 192 SESSHNKDSARDQVLSEVAKLVSMYQDEELSITVTGHSLGAALATLNAFDIVENGYNRAP 251

Query: 331 NGRVV----PVSVFSFSGPRVGNFSFKERL---QELGVKVLRVVNVHDVVPKTPGFLFNE 390
                    PV+ F F+ PRVG   FK R    + LG+++LRV N  DVVP+ P      
Sbjct: 252 RAAAAAAGCPVTAFVFASPRVGGHGFKRRFDGARGLGLRLLRVRNARDVVPRYP------ 311

Query: 391 SVPTAVMKFAEELPWSYSHVGVELKLDHKISPFLKDTNDPVCAHNLEAHLHLLDGYH-GK 450
             P             Y  VG EL +D   SP+L+   + +  HNLE +LH + G   G+
Sbjct: 312 --PAP----------PYHGVGTELAIDTGESPYLRRPGNELVWHNLECYLHGVAGARGGE 371

Query: 451 GRRFVLTSGRDPALVNKACDFLKDHYLVPPNWRQDENKGMIRNKDGRWIQPDRSKFDDHP 499
             RF L   RD AL NK+   L+D + VP  W    N+GM+R  DGRW   DR + +D  
Sbjct: 372 AGRFKLAVERDVALANKSYGALRDEHAVPAGWWIPSNRGMVRGADGRWTLMDREEDEDSA 411

BLAST of Carg25777-RA vs. Swiss-Prot
Match: sp|B9EYD3|PLA4_ORYSJ (Phospholipase A1-II 4 OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0652300 PE=2 SV=2)

HSP 1 Score: 308.9 bits (790), Expect = 1.0e-82
Identity = 171/406 (42.12%), Postives = 239/406 (58.87%), Query Frame = 0

Query: 90  IANSWREIHGSNDWTGLLDPMNDLLRSEIIRYGEMAQSCYDAFDYDPFSKYCGSCRFSRS 149
           +A  WRE+HG + W GLLDP++  LR  +I YGE+AQ+  DAF  + +S + G+CR+SR 
Sbjct: 9   VAERWRELHGEDHWKGLLDPLDADLRRSVIGYGELAQATNDAFIREAWSPHAGACRYSRD 68

Query: 150 KFFERLGME---SGGYEVTRYLYATSNI-NLPNFFKKSRWPKVWSKNANWIGYVAVSNDA 209
           +F E+       +G YEVT + YAT+    +P  F       V ++ +NW+GYVAV+ DA
Sbjct: 69  RFLEKAQASTQLAGLYEVTAFFYATAGAGGVPAPF------MVRNRESNWMGYVAVATDA 128

Query: 210 QSKLLGRRDIAIAWRGTVTRLEWIVDLMDFLKPVAAV-----KIPCPNLAVKAESGFLAL 269
               LGRRD+ +AWRGTV  +EW+ DL   L   A V     + P P    +   G+L++
Sbjct: 129 GVAALGRRDVVVAWRGTVRPMEWLNDLDFTLVSAAGVLGAGGRSPAP----RVHRGWLSI 188

Query: 270 YTDKDEGCGYSKLSAREQIVTEVKRLVERFAGEEMSITITGHSLGSALAVLSGFDVAEMG 329
           YT  D    YSKLSAREQI  E+KRL++++  EE SIT+ GHSLG+A+A L+  D+   G
Sbjct: 189 YTASDPASKYSKLSAREQISDEIKRLMDKYKDEETSITVVGHSLGAAVATLNAADIVSNG 248

Query: 330 VNRLENGRVVPVSVFSFSGPRVGNFSFKERLQEL-GVKVLRVVNVHDVVPKTPGFLFNES 389
           +N  ++G   PV+  +F+ PRVG+  F++   EL G+++LRV N  DVVPK P       
Sbjct: 249 LN--QHG-ACPVTAVAFACPRVGDSGFRKLFDELPGLRLLRVCNSPDVVPKYP------- 308

Query: 390 VPTAVMKFAEELPWSYSHVGVELKLDHKISPFLKDTNDPVCAHNLEAHLHLLDGYHGKGR 449
                       P  Y+ VGVEL +D + SP+LK   +    H+LE ++H + G  GK  
Sbjct: 309 ------------PMGYADVGVELPVDTRRSPYLKSPGNQAVWHSLECYMHGVAGAQGKRG 368

Query: 450 RFVLTSGRDPALVNKACDFLKDHYLVPPNWRQDENKGMIRNKDGRW 486
            F L   RD ALVNK  D LK+ Y VPP+W    +KGM+R  DG W
Sbjct: 369 GFKLEVDRDVALVNKNVDALKEEYHVPPSWSVQRDKGMVRGADGHW 382

BLAST of Carg25777-RA vs. TrEMBL
Match: tr|A0A0A0K5I4|A0A0A0K5I4_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G378470 PE=4 SV=1)

HSP 1 Score: 797.3 bits (2058), Expect = 2.0e-227
Identity = 397/517 (76.79%), Postives = 436/517 (84.33%), Query Frame = 0

Query: 1   MAIHLSNVLF-FPITNSLHQSKQSSSSLHFSTSNRHFRGFRLFSTRRTQRRRQSTYEVAS 60
           MAI LSN LF FP        KQ SS L +STSN  F+  RLF TR  ++  Q   +V S
Sbjct: 1   MAILLSNFLFPFP--------KQFSSPLPYSTSNSWFQTSRLFKTRNAEQSSQC--QVIS 60

Query: 61  RTRDTSSIITDLEKQTEGDEHTENESVAAEIANSWREIHGSNDWTGLLDPMNDLLRSEII 120
           RTR + + I + E+Q +   + E     ++IA+SWREIHGSNDWTGLLDPMNDLLRSE+I
Sbjct: 61  RTRHSYASIEEHEQQQQ--INMEESLPESKIADSWREIHGSNDWTGLLDPMNDLLRSELI 120

Query: 121 RYGEMAQSCYDAFDYDPFSKYCGSCRFSRSKFFERLGMESGGYEVTRYLYATSNINLPNF 180
           RYGEM+QSCYDAFDYDPFSKYCGSCRFSR KFFERLGME+ GYEVTRYLYATSNIN+PNF
Sbjct: 121 RYGEMSQSCYDAFDYDPFSKYCGSCRFSRGKFFERLGMENVGYEVTRYLYATSNINMPNF 180

Query: 181 FKKSRWPKVWSKNANWIGYVAVSNDAQSKLLGRRDIAIAWRGTVTRLEWIVDLMDFLKPV 240
           FKKSRWPKVWSK+ANWIGYVAVSND +SK LGRRDI +AWRGTVTRLEWI DLMDFLKP+
Sbjct: 181 FKKSRWPKVWSKSANWIGYVAVSNDEKSKELGRRDIVVAWRGTVTRLEWITDLMDFLKPI 240

Query: 241 AAVKIPCPNLAVKAESGFLALYTDK-DEGCGYSKLSAREQIVTEVKRLVERFAG--EEMS 300
           AA KI CPNL VK ESGF+ LYT+K +EGCGY + SAREQ++ EVKRL ERF G  EEMS
Sbjct: 241 AAAKIGCPNLGVKVESGFVDLYTEKEEEGCGYCRFSAREQVMAEVKRLTERFGGAEEEMS 300

Query: 301 ITITGHSLGSALAVLSGFDVAEMGVNRLENGRVVPVSVFSFSGPRVGNFSFKERLQELGV 360
           ITITGHSLGSALAVLS FD+AE G+NRL NGRVVPV VFSFSGPRVGNFSFKERL ELGV
Sbjct: 301 ITITGHSLGSALAVLSAFDLAETGLNRLGNGRVVPVCVFSFSGPRVGNFSFKERLHELGV 360

Query: 361 KVLRVVNVHDVVPKTPGFLFNESVPTAVMKFAEELPWSYSHVGVELKLDHKISPFLKDTN 420
           KVLRV+N+HD+VPK+PGFL NES+P AVM++AE LPWSYSHVGVELKLDHK+SPFLK TN
Sbjct: 361 KVLRVINIHDIVPKSPGFLLNESIPRAVMQYAEGLPWSYSHVGVELKLDHKVSPFLKQTN 420

Query: 421 DPVCAHNLEAHLHLLDGYHGKGRRFVLTSGRDPALVNKACDFLKDHYLVPPNWRQDENKG 480
           DPVCAHNLEA LHLLDGYH K  RFVL SGRDPALVNK CDFLKDHYLVPPNWRQDENKG
Sbjct: 421 DPVCAHNLEALLHLLDGYHEKDGRFVLASGRDPALVNKGCDFLKDHYLVPPNWRQDENKG 480

Query: 481 MIRNKDGRWIQPDRSKFDDHPEDIHHHLKQLGLTFDS 514
           MIRNKDGRWIQPDR KF+DHP DIHHHL QLGL F S
Sbjct: 481 MIRNKDGRWIQPDRLKFEDHPHDIHHHLTQLGLHFYS 505

BLAST of Carg25777-RA vs. TrEMBL
Match: tr|A0A1S3BQI0|A0A1S3BQI0_CUCME (phospholipase A1-Igamma1, chloroplastic-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103492623 PE=4 SV=1)

HSP 1 Score: 777.7 bits (2007), Expect = 1.6e-221
Identity = 386/517 (74.66%), Postives = 420/517 (81.24%), Query Frame = 0

Query: 1   MAIHLSNVLF-FPITNSLHQSKQSSSSLHFSTSNRHFRGFRLFSTRRTQRRRQSTYEVAS 60
           MAI LSN LF FP    LH                            T++  Q   +V S
Sbjct: 1   MAILLSNFLFLFP--KQLHHXXXXXXXXXXXXXXXXXXXXXXXXXXNTEQSSQC--QVIS 60

Query: 61  RTRDTSSIITDLEKQTEGDEHTENESVAAEIANSWREIHGSNDWTGLLDPMNDLLRSEII 120
           RTRD+ +   +         + E     ++IA+SWREIHGSNDWTGLLDPMNDLLRSE+I
Sbjct: 61  RTRDSYAFTEEHXXXXXXXINMEESLPESKIADSWREIHGSNDWTGLLDPMNDLLRSELI 120

Query: 121 RYGEMAQSCYDAFDYDPFSKYCGSCRFSRSKFFERLGMESGGYEVTRYLYATSNINLPNF 180
           RYGEM+QSCYDAFDYDPFSKYCGSCRFSR KFFERLGME+ GYEVTRYLYATSNIN+PNF
Sbjct: 121 RYGEMSQSCYDAFDYDPFSKYCGSCRFSRGKFFERLGMENVGYEVTRYLYATSNINMPNF 180

Query: 181 FKKSRWPKVWSKNANWIGYVAVSNDAQSKLLGRRDIAIAWRGTVTRLEWIVDLMDFLKPV 240
           FKKSRWPKVWSK+ANWIGYVAVSND +SK LGRRDI +AWRGTVTRLEWI DLMDFLKP+
Sbjct: 181 FKKSRWPKVWSKSANWIGYVAVSNDEKSKELGRRDIVVAWRGTVTRLEWITDLMDFLKPI 240

Query: 241 AAVKIPCPNLAVKAESGFLALYTDKDE-GCGYSKLSAREQIVTEVKRLVERFAG--EEMS 300
           A  KI CPN  VK ESGF+ LYT+K+E GCGY + SAREQ++ EVKRL ERF G  EEMS
Sbjct: 241 AEAKIGCPNSGVKVESGFVDLYTEKEEKGCGYCRFSAREQVMAEVKRLTERFGGAEEEMS 300

Query: 301 ITITGHSLGSALAVLSGFDVAEMGVNRLENGRVVPVSVFSFSGPRVGNFSFKERLQELGV 360
           ITITGHSLGSALAVLSGFD+AE G+NRL NGR+VPV VFSFSGPRVGNFSFKE L ELGV
Sbjct: 301 ITITGHSLGSALAVLSGFDIAETGLNRLGNGRLVPVCVFSFSGPRVGNFSFKEHLHELGV 360

Query: 361 KVLRVVNVHDVVPKTPGFLFNESVPTAVMKFAEELPWSYSHVGVELKLDHKISPFLKDTN 420
           KVLRVVN+HD+VPKTPGFLFNES+P AVM+FAE LPWSYSHVGVELKLDHK+SPFLK TN
Sbjct: 361 KVLRVVNIHDIVPKTPGFLFNESIPRAVMQFAEGLPWSYSHVGVELKLDHKVSPFLKQTN 420

Query: 421 DPVCAHNLEAHLHLLDGYHGKGRRFVLTSGRDPALVNKACDFLKDHYLVPPNWRQDENKG 480
           DPVCAHNLEA LHLLDGYHGKG RFVL SGRDPALVNK CDFLKDHYLVPPNWRQDENKG
Sbjct: 421 DPVCAHNLEALLHLLDGYHGKGGRFVLASGRDPALVNKGCDFLKDHYLVPPNWRQDENKG 480

Query: 481 MIRNKDGRWIQPDRSKFDDHPEDIHHHLKQLGLTFDS 514
           MIRN+DGRWIQPDR KFDDHP+DIHHHL QLGL F S
Sbjct: 481 MIRNQDGRWIQPDRLKFDDHPQDIHHHLTQLGLHFYS 513

BLAST of Carg25777-RA vs. TrEMBL
Match: tr|A0A251N298|A0A251N298_PRUPE (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_8G231900 PE=4 SV=1)

HSP 1 Score: 721.5 bits (1861), Expect = 1.4e-204
Identity = 344/468 (73.50%), Postives = 391/468 (83.55%), Query Frame = 0

Query: 53  STYEVASRTRDT-SSIITDLEKQT--EGDEHTENESV-----AAEIANSWREIHGSNDWT 112
           ST  + S+T D+ +SIIT+LEK++  + D++   E +        +++ WRE+HG +DW 
Sbjct: 42  STKALHSKTSDSLTSIITELEKESLNKDDQNQRQEDINTTHHERRLSDVWRELHGQDDWV 101

Query: 113 GLLDPMNDLLRSEIIRYGEMAQSCYDAFDYDPFSKYCGSCRFSRSKFFERLGMESGGYEV 172
           GLLDPM+ LLRSE+IRYGEMAQ+CYDAFD+DPFSKYCGSCRF R  FF  LGM   GY V
Sbjct: 102 GLLDPMDPLLRSELIRYGEMAQACYDAFDFDPFSKYCGSCRFVRRSFFPSLGMAHHGYHV 161

Query: 173 TRYLYATSNINLPNFFKKSRWPKVWSKNANWIGYVAVSNDAQSKLLGRRDIAIAWRGTVT 232
           +RYL+ATSNINLPNFFKKSRWPKVWSKNANWIGYVAVS+D  +  LGRRDI+IAWRGTVT
Sbjct: 162 SRYLFATSNINLPNFFKKSRWPKVWSKNANWIGYVAVSDDETTARLGRRDISIAWRGTVT 221

Query: 233 RLEWIVDLMDFLKPVAAVKIPCPNLAVKAESGFLALYTDKDEGCGYSKLSAREQIVTEVK 292
           RLEWIVDLMDFLKPV+  KIPCP+  VK ESGFL LYTDKDE C YS  SAREQI+TE+K
Sbjct: 222 RLEWIVDLMDFLKPVSGNKIPCPDRTVKVESGFLDLYTDKDETCRYSTFSAREQILTEIK 281

Query: 293 RLVERFAGEEMSITITGHSLGSALAVLSGFDVAEMGVNRLENGRVVPVSVFSFSGPRVGN 352
           RLVE ++GEE+SITITGHSLGSALA+LS +D+ E G+N   +G+VVPVSV SFSGPRVGN
Sbjct: 282 RLVEEYSGEELSITITGHSLGSALAILSSYDITETGLNVTADGQVVPVSVLSFSGPRVGN 341

Query: 353 FSFKERLQELGVKVLRVVNVHDVVPKTPGFLFNESVPTAVMKFAEELPWSYSHVGVELKL 412
             FKERL+ LGVKVLRVVNVHDVVPK+PG  FNE V   +MK AE LPWSYSHVGV+L+L
Sbjct: 342 VRFKERLESLGVKVLRVVNVHDVVPKSPGLFFNEHVAPRLMKLAEGLPWSYSHVGVQLEL 401

Query: 413 DHKISPFLKDTNDPVCAHNLEAHLHLLDGYHGKGRRFVLTSGRDPALVNKACDFLKDHYL 472
           DH  SPFLK T+DPVCAHNLEAHLHLLDGYHGKG RFVL SGRDPALVNKA DFLKDHYL
Sbjct: 402 DHTHSPFLKQTSDPVCAHNLEAHLHLLDGYHGKGHRFVLASGRDPALVNKASDFLKDHYL 461

Query: 473 VPPNWRQDENKGMIRNKDGRWIQPDRSKFDDHPEDIHHHLKQLGLTFD 513
           VPP WRQDENKGM+R+KDGRWIQP+R K DDHPEDIHHHLKQLGL  D
Sbjct: 462 VPPYWRQDENKGMVRSKDGRWIQPERPKLDDHPEDIHHHLKQLGLASD 509

BLAST of Carg25777-RA vs. TrEMBL
Match: tr|M5W0K0|M5W0K0_PRUPE (Uncharacterized protein (Fragment) OS=Prunus persica OX=3760 GN=PRUPE_ppa017780mg PE=4 SV=1)

HSP 1 Score: 719.9 bits (1857), Expect = 4.0e-204
Identity = 343/467 (73.45%), Postives = 390/467 (83.51%), Query Frame = 0

Query: 54  TYEVASRTRDT-SSIITDLEKQT--EGDEHTENESV-----AAEIANSWREIHGSNDWTG 113
           T  + S+T D+ +SIIT+LEK++  + D++   E +        +++ WRE+HG +DW G
Sbjct: 1   TKALHSKTSDSLTSIITELEKESLNKDDQNQRQEDINTTHHERRLSDVWRELHGQDDWVG 60

Query: 114 LLDPMNDLLRSEIIRYGEMAQSCYDAFDYDPFSKYCGSCRFSRSKFFERLGMESGGYEVT 173
           LLDPM+ LLRSE+IRYGEMAQ+CYDAFD+DPFSKYCGSCRF R  FF  LGM   GY V+
Sbjct: 61  LLDPMDPLLRSELIRYGEMAQACYDAFDFDPFSKYCGSCRFVRRSFFPSLGMAHHGYHVS 120

Query: 174 RYLYATSNINLPNFFKKSRWPKVWSKNANWIGYVAVSNDAQSKLLGRRDIAIAWRGTVTR 233
           RYL+ATSNINLPNFFKKSRWPKVWSKNANWIGYVAVS+D  +  LGRRDI+IAWRGTVTR
Sbjct: 121 RYLFATSNINLPNFFKKSRWPKVWSKNANWIGYVAVSDDETTARLGRRDISIAWRGTVTR 180

Query: 234 LEWIVDLMDFLKPVAAVKIPCPNLAVKAESGFLALYTDKDEGCGYSKLSAREQIVTEVKR 293
           LEWIVDLMDFLKPV+  KIPCP+  VK ESGFL LYTDKDE C YS  SAREQI+TE+KR
Sbjct: 181 LEWIVDLMDFLKPVSGNKIPCPDRTVKVESGFLDLYTDKDETCRYSTFSAREQILTEIKR 240

Query: 294 LVERFAGEEMSITITGHSLGSALAVLSGFDVAEMGVNRLENGRVVPVSVFSFSGPRVGNF 353
           LVE ++GEE+SITITGHSLGSALA+LS +D+ E G+N   +G+VVPVSV SFSGPRVGN 
Sbjct: 241 LVEEYSGEELSITITGHSLGSALAILSSYDITETGLNVTADGQVVPVSVLSFSGPRVGNV 300

Query: 354 SFKERLQELGVKVLRVVNVHDVVPKTPGFLFNESVPTAVMKFAEELPWSYSHVGVELKLD 413
            FKERL+ LGVKVLRVVNVHDVVPK+PG  FNE V   +MK AE LPWSYSHVGV+L+LD
Sbjct: 301 RFKERLESLGVKVLRVVNVHDVVPKSPGLFFNEHVAPRLMKLAEGLPWSYSHVGVQLELD 360

Query: 414 HKISPFLKDTNDPVCAHNLEAHLHLLDGYHGKGRRFVLTSGRDPALVNKACDFLKDHYLV 473
           H  SPFLK T+DPVCAHNLEAHLHLLDGYHGKG RFVL SGRDPALVNKA DFLKDHYLV
Sbjct: 361 HTHSPFLKQTSDPVCAHNLEAHLHLLDGYHGKGHRFVLASGRDPALVNKASDFLKDHYLV 420

Query: 474 PPNWRQDENKGMIRNKDGRWIQPDRSKFDDHPEDIHHHLKQLGLTFD 513
           PP WRQDENKGM+R+KDGRWIQP+R K DDHPEDIHHHLKQLGL  D
Sbjct: 421 PPYWRQDENKGMVRSKDGRWIQPERPKLDDHPEDIHHHLKQLGLASD 467

BLAST of Carg25777-RA vs. TrEMBL
Match: tr|A0A2P5FB63|A0A2P5FB63_9ROSA (Fungal lipase-like domain containing protein OS=Trema orientalis OX=63057 GN=TorDALL3 PE=4 SV=1)

HSP 1 Score: 715.7 bits (1846), Expect = 7.5e-203
Identity = 334/454 (73.57%), Postives = 380/454 (83.70%), Query Frame = 0

Query: 64  TSSIITDLEKQTEGDEHTENESVAAE--------IANSWREIHGSNDWTGLLDPMNDLLR 123
           T + ITDL K  +     + E +  +        ++  WRE+HG +DW GLLDPM+ LLR
Sbjct: 55  TPTNITDLHKTEQRTNRNQEEDIMVKQHEPERRRLSEVWRELHGQDDWVGLLDPMDPLLR 114

Query: 124 SEIIRYGEMAQSCYDAFDYDPFSKYCGSCRFSRSKFFERLGMESGGYEVTRYLYATSNIN 183
           SE++RYGEMAQ+CYDAFD+D FSKYCGSCRF R KFFE LGM    Y+V+RYLYATSNIN
Sbjct: 115 SELVRYGEMAQACYDAFDFDSFSKYCGSCRFIRRKFFESLGMAHHDYDVSRYLYATSNIN 174

Query: 184 LPNFFKKSRWPKVWSKNANWIGYVAVSNDAQSKLLGRRDIAIAWRGTVTRLEWIVDLMDF 243
           LPNFFKKSRWPKVWS++ANWIGYVAVS D  ++ LGRRD+ IAWRGTVTRLEWI DLMDF
Sbjct: 175 LPNFFKKSRWPKVWSRSANWIGYVAVSTDEATERLGRRDVTIAWRGTVTRLEWIADLMDF 234

Query: 244 LKPVAAVKIPCPNLAVKAESGFLALYTDKDEGCGYSKLSAREQIVTEVKRLVERFAGEEM 303
           LKP++  KIPCP+  VK ESGF+ LYTDKDE C + K SAREQI+TEVKRLVE + GEE+
Sbjct: 235 LKPISGNKIPCPDPTVKVESGFVDLYTDKDEACRFCKYSAREQILTEVKRLVEMYPGEEL 294

Query: 304 SITITGHSLGSALAVLSGFDVAEMGVNRLENGRVVPVSVFSFSGPRVGNFSFKERLQELG 363
           SITITGHSLG+ALA+LS +D+AE G+N   NGRVVPVSVFSFSGPRVGN  FKERL+ LG
Sbjct: 295 SITITGHSLGAALAILSAYDIAETGINVNGNGRVVPVSVFSFSGPRVGNIRFKERLEALG 354

Query: 364 VKVLRVVNVHDVVPKTPGFLFNESVPTAVMKFAEELPWSYSHVGVELKLDHKISPFLKDT 423
           VKVLRVVNVHDVVPK+PG +FNESVP  +MK AE LPWSYSHVGVEL+LDHK SPFLK T
Sbjct: 355 VKVLRVVNVHDVVPKSPGLVFNESVPPILMKIAEGLPWSYSHVGVELELDHKNSPFLKPT 414

Query: 424 NDPVCAHNLEAHLHLLDGYHGKGRRFVLTSGRDPALVNKACDFLKDHYLVPPNWRQDENK 483
           NDPVC+HNLEAHLHLLDGYHGKG RFVL+SGRDPALVNKA DFLKDHYLVPP WRQDENK
Sbjct: 415 NDPVCSHNLEAHLHLLDGYHGKGHRFVLSSGRDPALVNKASDFLKDHYLVPPFWRQDENK 474

Query: 484 GMIRNKDGRWIQPDRSKFDDHPEDIHHHLKQLGL 510
           GMIR+KDGRW+QP+R K DDHPED+H HLKQ+GL
Sbjct: 475 GMIRSKDGRWVQPERPKLDDHPEDMHRHLKQIGL 508

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022959614.12.1e-30299.42phospholipase A1-Igamma1, chloroplastic [Cucurbita moschata][more]
XP_023514756.14.9e-29998.64phospholipase A1-Igamma1, chloroplastic [Cucurbita pepo subsp. pepo][more]
XP_023004255.12.1e-29797.66phospholipase A1-Igamma1, chloroplastic [Cucurbita maxima][more]
XP_004146952.13.0e-22776.79PREDICTED: phospholipase A1-Igamma1, chloroplastic [Cucumis sativus] >KGN44758.1... [more]
XP_008451275.12.4e-22174.66PREDICTED: phospholipase A1-Igamma1, chloroplastic-like isoform X1 [Cucumis melo... [more]
Match NameE-valueIdentityDescription
AT1G06800.11.8e-18166.11alpha/beta-Hydrolases superfamily protein[more]
AT2G30550.24.2e-17563.27alpha/beta-Hydrolases superfamily protein[more]
AT1G51440.17.7e-14559.21alpha/beta-Hydrolases superfamily protein[more]
AT1G30370.11.8e-10944.42alpha/beta-Hydrolases superfamily protein[more]
AT2G31690.12.2e-8343.73alpha/beta-Hydrolases superfamily protein[more]
Match NameE-valueIdentityDescription
sp|Q941F1|PLA15_ARATH3.2e-18066.11Phospholipase A1-Igamma1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At1g0... [more]
sp|Q3EBR6|PLA16_ARATH7.5e-17463.27Phospholipase A1-Igamma2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At2g3... [more]
sp|Q9C8J6|PLA17_ARATH1.4e-14359.21Phospholipase A1-Igamma3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At1g5... [more]
sp|Q6F358|PLA6_ORYSJ5.5e-8442.28Phospholipase A1-II 6 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0574000 P... [more]
sp|B9EYD3|PLA4_ORYSJ1.0e-8242.12Phospholipase A1-II 4 OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0652300 P... [more]
Match NameE-valueIdentityDescription
tr|A0A0A0K5I4|A0A0A0K5I4_CUCSA2.0e-22776.79Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G378470 PE=4 SV=1[more]
tr|A0A1S3BQI0|A0A1S3BQI0_CUCME1.6e-22174.66phospholipase A1-Igamma1, chloroplastic-like isoform X1 OS=Cucumis melo OX=3656 ... [more]
tr|A0A251N298|A0A251N298_PRUPE1.4e-20473.50Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_8G231900 PE=4 SV=1[more]
tr|M5W0K0|M5W0K0_PRUPE4.0e-20473.45Uncharacterized protein (Fragment) OS=Prunus persica OX=3760 GN=PRUPE_ppa017780m... [more]
tr|A0A2P5FB63|A0A2P5FB63_9ROSA7.5e-20373.57Fungal lipase-like domain containing protein OS=Trema orientalis OX=63057 GN=Tor... [more]
The following terms have been associated with this mRNA:
Vocabulary: Biological Process
TermDefinition
GO:0006629lipid metabolic process
Vocabulary: INTERPRO
TermDefinition
IPR029058AB_hydrolase
IPR002921Fungal_lipase-like
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006629 lipid metabolic process
cellular_component GO:0005575 cellular_component
molecular_function GO:0003674 molecular_function

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Carg25777Carg25777gene


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Carg25777-RACarg25777-RA-proteinpolypeptide


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg25777-RA.exon.1Carg25777-RA.exon.1exon
Carg25777-RA.exon.2Carg25777-RA.exon.2exon
Carg25777-RA.exon.3Carg25777-RA.exon.3exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg25777-RA.CDS.1Carg25777-RA.CDS.1CDS
Carg25777-RA.CDS.2Carg25777-RA.CDS.2CDS
Carg25777-RA.CDS.3Carg25777-RA.CDS.3CDS


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg25777-RA.three_prime_UTR.1Carg25777-RA.three_prime_UTR.1three_prime_UTR


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3DG3DSA:3.40.50.12520coord: 90..501
e-value: 1.8E-157
score: 526.4
NoneNo IPR availablePANTHERPTHR31403:SF6PHOSPHOLIPASE A1-IGAMMA3, CHLOROPLASTICcoord: 16..505
NoneNo IPR availablePANTHERPTHR31403FAMILY NOT NAMEDcoord: 16..505
NoneNo IPR availableCDDcd00519Lipase_3coord: 148..406
e-value: 1.74692E-45
score: 159.564
IPR002921Fungal lipase-like domainPFAMPF01764Lipase_3coord: 217..374
e-value: 3.0E-35
score: 121.1
IPR029058Alpha/Beta hydrolase foldSUPERFAMILYSSF53474alpha/beta-Hydrolasescoord: 122..130
coord: 161..435