Carg25350-RA (mRNA) Silver-seed gourd

NameCarg25350-RA
TypemRNA
OrganismCucurbita argyrosperma (Silver-seed gourd)
Description65-kDa microtubule-associated protein 1
LocationCucurbita_argyrosperma_scaffold_246 : 117980 .. 123057 (+)
Sequence length3122
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TCTTCGCCAACTCTTTTCAATCGTTCGTCTAATGCCTTACATCGCCTTGAGCTGCCAATCTTTGAACTCTTTGATTACAAGGGGACTATTTTTAGATCTGAAATTTTCGTCTCACATGCTTGAGCTTCGAGGATCATTCTAGTTCCCATCTTTCACTGCTGCTCTTCTTTTTCTTTTTTCTGACGCTGCTCTGTGCCTTCCATTTGATTTTTCCAGTTCCTTCTGTTTGAATGTGATGGTACTGGGAGAGAGTAGAGTGTCCCCAACTCTTTGATGCCCTTGATTGTATGAAATGTAGGGTTTAAATTTGGTTGCGATTCTTCATTTCGTGTGTATATGAGAAATGGGAGATTTGCACATTTTTTGTTAACTAAATTCTCAGAACCCCATGGTGAACTTTTGTGATTGGGAGATTGAGTTGGAGTCTTGATCTCAAAAACTTTGTTCTGGATTCTTGGGCTGTGTCTTTCGTTTGGTGGCTTATTTGGTTGATTTAGTTCTCTTACATGAAATGAAGTACTTAAATTTTGAATAATCTAGCTCTTGGGTTATGAGTAGAACAAATTGTTAACTGATCGGTGATGAAATGTCAGTTAAGTTTATGAATGTTTGCTTCTTTCTCTCACACACTCAAATACACACACCATGCACGGACTGCATATTTTCACAGAGATTATTGGAGGTAACTTGTATTGGTTCTGTAGTTCCATTCTTCCATATACTTGTTCTTTTTTAATGATATCAAAGACACATTTTGTGTGTGGATGGGGATGACACATTCTCGGGGCTTAATGTTGCATGATTTCATTTCATATGAACAACCTAACTTACAGGGTCTGATTAATGCAGATAAAAGTGTGAATTTTACAGATTCGTGAATGGCGGTGGAAGATACACAAAATCCTCTTCAAGGGGAAACCACATGTGGTACCCTACTGCAGAAACTGCAAGTAAATAAATGACGCTGTTGGTCTTTTGCTTCCCTTTTTTCCGTCTAAATATTTTCTATTGGTCTCACCAAAAGTTATCTCTTAGGAAATTTGGGATGAAGTTGGTGAAAGTGATGAGGATCGAGATAAAATGCTGCTTCAAATAGATCAGGAATGTTTGGATGTCTACAAAAGGAAAGTTGAGCTGGCTGATAAGTCAAGGGCGAAACTTCTGCAGTCTTTATCAGATGCAAAGCATGAGCTCTCCAATCTTCTATCTGCTCTTGGAGAAAAAAGTTTTTCTGAATTTGTGAGTGAATACTTCCATATAAGGCCGCCTTTTACATTTGAAAATGTTGCTGCATTTTTCTCATGATTCATATCAATTTTTTTTTTTTTTTTTTACAGCCTGAGAGTGCTTCTGGAACAATCAAGGAACAGCTTGCAGCCATAGCACCCGCTCTTGAACAGCTCTGGAAACAGAAAGAGAAGAGGGTTAAGGAATTTTTTGATGTGCAATCAGAGATACAGAAGATATGTGGAGAGATCACTGGGAGTTTAAACATTAGCGAGAATCCAGTAGTAGATGAGTCTCAACTTTCTGTGGAAAAGCTTGACGAATACCATTGCCAACTTCAAGAGTTGCAGAAAGAAAAGGTAAGTTTTAGTTTGAGGAGCTATATATTTTGTTTATCGTTCTTTCATCAAGCTAATCTTGGATCAATTATTCAAAGAACAGAGTGAAAGGTTGCACAAAGTTCTTGAATTTGTGAGCACCGTGCATGATCTCTGTGCTGTCCTTGGGATTGATTTCTTTGGTACTGTAACTGAGGTTCACCCAAGCTTAAACGACGCAACTGGTGTGCAATCTAAAAGTATTAGCAATGATACTTTATCCAAGCTAGCAAAGATGGTCTTAGCACTGAAAGAAGACAAAAAGCAGAGGCTACATAAGGTGAATTTAATATTCTGAACTTGTTTCTTTAAGGATGAGTTTGGCGCAACATCTAATTTAAACAATTCACTTCTTTTTTCCTCAAAACACTTTTTGTAAAACTAGTTTTAATTAAAATACTTGTGTGTTTGGTTACTTTTTCTTCAGAGTCATTTTAATCCAAAGTTCATTTGGCTGCATTGCATCTAAAAGTGATTCTACAAAGGCCATCATAAAGTAATTCTAAGCGATTACCAATCAACTATTTTATAATATCTCTTTTTGGATGATCAACTTTAAAATGGTTCTTCACAAAATGATTTTAAACTAATTTGATTGTTTCATAAGTGATTTTTATTTGTTACCGAGCACTCAAGATTCTTTATAGAACACTTTGGGCCTTTCAAAATCACTTATGAAGTTAATCCAAGCACCCACTAAGAAGAGAATTTCAAAGTTACGTTTGATTTGCCTCCTTGCTGCAAAATATGAAAATAGGTATTTATGTGGATGTGAAACATCTACGTAATATCAATCAAGCTTACCATTTAAAATGATATTTAAATAAACTTGTTATGGTATTCAATTTAGTTTGATAATTTAAAATCTTCCCACTAATATGCATCCTCATTTTTATCATTTAAAAGGATATTAGAATTACAATTACTTGTGGCATTGTATCTCGATTGAATTTATTGCTTTAGTCATACTAAAGTGGTAAACAGAAGTATGTCATGCACTCGGATTGTTTCTTAGCTTGGTATGTCTTTTCTTCTTTATTTGCAACTAGCACGTATAACAATGCATGGAAACTAGAGCTTTTTAGAGTTTGAAACAATGCATAGGCAGCTTACTATTTTTCAACAGCACTTATACATATCAGTTACAAGGATAAAGATTTGTACATTCTCATACATGCGCACTATTTCTAACTCAAGATTATGATGATATTCGTTTAGTTTTTTTTGTGTAGACCTTTTAATCTTTGTATTTTATTGTATTGTACTTTCATATACAGCTTCAAGAATTGGCAACTCAGCTGGTTGATCTTTGGAATTTAATGGACACCCCCTCGGAAGAAAGAAGTTTATTTGATCATGTCACGTGTAACATATCAGCATCAGTAGATGAAGTGACTGTCCCTGGGGCTCTTGCTCTTGATTTGATTGAGCAGGTATTTAAGTGTTCTTTATTTCTCTTGCTGTAAATTTGTACAGGTGTGGGTGTTGACTCTTTTTTGTACTGCATGCTAAATATAAATAAGGCTGAGGTGGAGGTTGAGAGGCTTGATCAACTCAAGGCCAGTAGGATGAAGGAAATTGCTTTCAAGAAGCAATCTGAACTTGAAGAGATATTTGCTCATGCTCACATAGAAATCGACCCAGAGGCAGCTCGAGAAAAAATTATGACACTGATTGATTCGGGTAATGTTGAGCCTTCTGAATTGCTGGATGACATGGACAATAAAATAGCAAAGGCCAAAGAAGAAGTTCTCAGCAGGAAAGAAATATTGGACAAGGTTGAAAAATGGATGTTAGCATGTGAAGAAGAGAGCTGGCTAGAAGACTACAATCGGGTACTTGTCCTTTCCCGCTATCATATTAAAACTATTAGCTAATAACCTAACTCTAAAAATTTGATTATGTTGATTTGCCTGTATGAAATCTTTTGATTTTGTGCTATTTTGTAATTCCCTGGACTAATTGATTGGAAAACTTACAGTTACAAATTTACTTCTTACGTGGTTGATATAGCTAAGTTCTAAACATCATCTCAATTTCACAGTTGAAAATTTGAATGTCGCTCCCAAAGAGACTGAGAGAGACTAAATGTATATGCTACTTTATTTTCTAAAAAATACTGGACTTGTGTTGTGTGTTTTGACGCCATTGGCATCACCTTGCAGGATGATAACAGGTATAATGCTAGCAGAGGGGCTCATTTGAATCTCAAACGTGCAGAGAAAGCTAGGATTCTGGTCAACAAAATTCCAGGTTTGTTGTATATATATATATGTCCGTACTTCAATATTGCTCTTACAATTCCGTGCATACTAATGCTTGGCTCTTGTTGATGACTTGATTATGAAGCTCTTGTTGACACTTTAATTGCCAAAACTCGGGCATGGGAGGAGGATCGTGGCATATCGTTTATTTACGATGGTGTTCCTCTTCTTGCTATGCTTGATGAATACACCATGCTCAGGCATGAAAGAGAGGAGGAAAAACGACGATTTAGGGTAAATTGTTTCTAGCCTCGTGACGTTGATCAACTTATTTGTGTACATGTTACTTTTGGTTTGACATTGCCTCAACTGAACACTAACACTTTGGACAGCTTTAATTTACTCATGTGAAATTTGAAGTTACCTGTTTTTTTTTTCCTCTTAGTGAGACCTCTTATGGTGGACATTGCAGGACCATAAAAAATTTCATGAGCAGCAAAGCACAGACCAAGAGAGCATCACGAATTCAAGGCCTAGCCCTGCTCGACCAATTGGTACGAAGAAGGCAGCTGGACCTCGTGCCAATGGAGCTGTGAACGGAACTCCTAGCAGCAGAAGGTTATCGCTTAATCAGAATGGATCTAGATCTATGAATAAAGATGGAAAAAGGGATGGTATGAGACCAATGACACCTGTGAAGTATGATGCAATCTCGAAAGAGGACTCTGGCTCGCACGTCTCAGGTACGGAGCCTGTTTCATCTTCACCATGAGATAGAGGATAGATGTACTGTACTGATAATAGGTCTATATAATACCATCTGTAATATTTGTAGCTGCTGGTGTAGTGGACACGTTCCACAAAATAACGGAAGGTTAATGATGAGAGTGGGCGGGTGTTCCTTGTAGTTGTGTATGTTAGAAGAAGAAGTTGGATGTTTGTGGGTTAATGTTAGTTATTTTGCCCTCAATCTTTTGTACTCTACGGTCCAGAAGTGGGGGCAATACAGCTCTTTCGTCGGGGCGAGTTCTTTTTGGTTTTTGACTAATCTACTTCTCAATTGGACTCTGATTTTGTGATCTCTGTTTGTGTATGCAAATTATGAGTTTTTGACGTATTCAAGACCTCCATGTTTGCTCAACCTCCGACTAAGTTCCGTCTATTCTTTTTCCTCTAAAATAGTTGTATTATTACAAAATGGACGATCCACCTCCCTTCGAGGCCTAGCGTCTTTACTGACACACTTCTTCATGGCCACCCTCTTCGTCGG

mRNA sequence

TCTTCGCCAACTCTTTTCAATCGTTCGTCTAATGCCTTACATCGCCTTGAGCTGCCAATCTTTGAACTCTTTGATTACAAGGGGACTATTTTTAGATCTGAAATTTTCGTCTCACATGCTTGAGCTTCGAGGATCATTCTAGTTCCCATCTTTCACTGCTGCTCTTCTTTTTCTTTTTTCTGACGCTGCTCTGTGCCTTCCATTTGATTTTTCCAGTTCCTTCTGTTTGAATGTGATGGTACTGGGAGAGAGTAGAGTGTCCCCAACTCTTTGATGCCCTTGATTGTATGAAATGTAGGGTTTAAATTTGGTTGCGATTCTTCATTTCGTGTGTATATGAGAAATGGGAGATTTGCACATTTTTTGTTAACTAAATTCTCAGAACCCCATGGTGAACTTTTGTGATTGGGAGATTGAGTTGGAGTCTTGATCTCAAAAACTTTGTTCTGGATTCTTGGGCTGTGTCTTTCGTTTGGTGGCTTATTTGGTTGATTTAGTTCTCTTACATGAAATGAAGTACTTAAATTTTGAATAATCTAGCTCTTGGGTTATGAGTAGAACAAATTGTTAACTGATCGGTGATGAAATGTCAGTTAAGTTTATGAATGTTTGCTTCTTTCTCTCACACACTCAAATACACACACCATGCACGGACTGCATATTTTCACAGAGATTATTGGAGGTAACTTGTATTGGTTCTGTAGTTCCATTCTTCCATATACTTGTTCTTTTTTAATGATATCAAAGACACATTTTGTGTGTGGATGGGGATGACACATTCTCGGGGCTTAATGTTGCATGATTTCATTTCATATGAACAACCTAACTTACAGGGTCTGATTAATGCAGATAAAAGTGTGAATTTTACAGATTCGTGAATGGCGGTGGAAGATACACAAAATCCTCTTCAAGGGGAAACCACATGTGGTACCCTACTGCAGAAACTGCAAGAAATTTGGGATGAAGTTGGTGAAAGTGATGAGGATCGAGATAAAATGCTGCTTCAAATAGATCAGGAATGTTTGGATGTCTACAAAAGGAAAGTTGAGCTGGCTGATAAGTCAAGGGCGAAACTTCTGCAGTCTTTATCAGATGCAAAGCATGAGCTCTCCAATCTTCTATCTGCTCTTGGAGAAAAAAGTTTTTCTGAATTTCCTGAGAGTGCTTCTGGAACAATCAAGGAACAGCTTGCAGCCATAGCACCCGCTCTTGAACAGCTCTGGAAACAGAAAGAGAAGAGGGTTAAGGAATTTTTTGATGTGCAATCAGAGATACAGAAGATATGTGGAGAGATCACTGGGAGTTTAAACATTAGCGAGAATCCAGTAGTAGATGAGTCTCAACTTTCTGTGGAAAAGCTTGACGAATACCATTGCCAACTTCAAGAGTTGCAGAAAGAAAAGAGTGAAAGGTTGCACAAAGTTCTTGAATTTGTGAGCACCGTGCATGATCTCTGTGCTGTCCTTGGGATTGATTTCTTTGGTACTGTAACTGAGGTTCACCCAAGCTTAAACGACGCAACTGGTGTGCAATCTAAAAGTATTAGCAATGATACTTTATCCAAGCTAGCAAAGATGGTCTTAGCACTGAAAGAAGACAAAAAGCAGAGGCTACATAAGCTTCAAGAATTGGCAACTCAGCTGGTTGATCTTTGGAATTTAATGGACACCCCCTCGGAAGAAAGAAGTTTATTTGATCATGTCACGTGTAACATATCAGCATCAGTAGATGAAGTGACTGTCCCTGGGGCTCTTGCTCTTGATTTGATTGAGCAGGCTGAGGTGGAGGTTGAGAGGCTTGATCAACTCAAGGCCAGTAGGATGAAGGAAATTGCTTTCAAGAAGCAATCTGAACTTGAAGAGATATTTGCTCATGCTCACATAGAAATCGACCCAGAGGCAGCTCGAGAAAAAATTATGACACTGATTGATTCGGGTAATGTTGAGCCTTCTGAATTGCTGGATGACATGGACAATAAAATAGCAAAGGCCAAAGAAGAAGTTCTCAGCAGGAAAGAAATATTGGACAAGGTTGAAAAATGGATGTTAGCATGTGAAGAAGAGAGCTGGCTAGAAGACTACAATCGGGATGATAACAGGTATAATGCTAGCAGAGGGGCTCATTTGAATCTCAAACGTGCAGAGAAAGCTAGGATTCTGGTCAACAAAATTCCAGCTCTTGTTGACACTTTAATTGCCAAAACTCGGGCATGGGAGGAGGATCGTGGCATATCGTTTATTTACGATGGTGTTCCTCTTCTTGCTATGCTTGATGAATACACCATGCTCAGGCATGAAAGAGAGGAGGAAAAACGACGATTTAGGGACCATAAAAAATTTCATGAGCAGCAAAGCACAGACCAAGAGAGCATCACGAATTCAAGGCCTAGCCCTGCTCGACCAATTGGTACGAAGAAGGCAGCTGGACCTCGTGCCAATGGAGCTGTGAACGGAACTCCTAGCAGCAGAAGGTTATCGCTTAATCAGAATGGATCTAGATCTATGAATAAAGATGGAAAAAGGGATGGTATGAGACCAATGACACCTGTGAAGTATGATGCAATCTCGAAAGAGGACTCTGGCTCGCACGTCTCAGGTACGGAGCCTGTTTCATCTTCACCATGAGATAGAGGATAGATGTACTGTACTGATAATAGGTCTATATAATACCATCTGTAATATTTGTAGCTGCTGGTGTAGTGGACACGTTCCACAAAATAACGGAAGGTTAATGATGAGAGTGGGCGGGTGTTCCTTGTAGTTGTGTATGTTAGAAGAAGAAGTTGGATGTTTGTGGGTTAATGTTAGTTATTTTGCCCTCAATCTTTTGTACTCTACGGTCCAGAAGTGGGGGCAATACAGCTCTTTCGTCGGGGCGAGTTCTTTTTGGTTTTTGACTAATCTACTTCTCAATTGGACTCTGATTTTGTGATCTCTGTTTGTGTATGCAAATTATGAGTTTTTGACGTATTCAAGACCTCCATGTTTGCTCAACCTCCGACTAAGTTCCGTCTATTCTTTTTCCTCTAAAATAGTTGTATTATTACAAAATGGACGATCCACCTCCCTTCGAGGCCTAGCGTCTTTACTGACACACTTCTTCATGGCCACCCTCTTCGTCGG

Coding sequence (CDS)

ATGGCGGTGGAAGATACACAAAATCCTCTTCAAGGGGAAACCACATGTGGTACCCTACTGCAGAAACTGCAAGAAATTTGGGATGAAGTTGGTGAAAGTGATGAGGATCGAGATAAAATGCTGCTTCAAATAGATCAGGAATGTTTGGATGTCTACAAAAGGAAAGTTGAGCTGGCTGATAAGTCAAGGGCGAAACTTCTGCAGTCTTTATCAGATGCAAAGCATGAGCTCTCCAATCTTCTATCTGCTCTTGGAGAAAAAAGTTTTTCTGAATTTCCTGAGAGTGCTTCTGGAACAATCAAGGAACAGCTTGCAGCCATAGCACCCGCTCTTGAACAGCTCTGGAAACAGAAAGAGAAGAGGGTTAAGGAATTTTTTGATGTGCAATCAGAGATACAGAAGATATGTGGAGAGATCACTGGGAGTTTAAACATTAGCGAGAATCCAGTAGTAGATGAGTCTCAACTTTCTGTGGAAAAGCTTGACGAATACCATTGCCAACTTCAAGAGTTGCAGAAAGAAAAGAGTGAAAGGTTGCACAAAGTTCTTGAATTTGTGAGCACCGTGCATGATCTCTGTGCTGTCCTTGGGATTGATTTCTTTGGTACTGTAACTGAGGTTCACCCAAGCTTAAACGACGCAACTGGTGTGCAATCTAAAAGTATTAGCAATGATACTTTATCCAAGCTAGCAAAGATGGTCTTAGCACTGAAAGAAGACAAAAAGCAGAGGCTACATAAGCTTCAAGAATTGGCAACTCAGCTGGTTGATCTTTGGAATTTAATGGACACCCCCTCGGAAGAAAGAAGTTTATTTGATCATGTCACGTGTAACATATCAGCATCAGTAGATGAAGTGACTGTCCCTGGGGCTCTTGCTCTTGATTTGATTGAGCAGGCTGAGGTGGAGGTTGAGAGGCTTGATCAACTCAAGGCCAGTAGGATGAAGGAAATTGCTTTCAAGAAGCAATCTGAACTTGAAGAGATATTTGCTCATGCTCACATAGAAATCGACCCAGAGGCAGCTCGAGAAAAAATTATGACACTGATTGATTCGGGTAATGTTGAGCCTTCTGAATTGCTGGATGACATGGACAATAAAATAGCAAAGGCCAAAGAAGAAGTTCTCAGCAGGAAAGAAATATTGGACAAGGTTGAAAAATGGATGTTAGCATGTGAAGAAGAGAGCTGGCTAGAAGACTACAATCGGGATGATAACAGGTATAATGCTAGCAGAGGGGCTCATTTGAATCTCAAACGTGCAGAGAAAGCTAGGATTCTGGTCAACAAAATTCCAGCTCTTGTTGACACTTTAATTGCCAAAACTCGGGCATGGGAGGAGGATCGTGGCATATCGTTTATTTACGATGGTGTTCCTCTTCTTGCTATGCTTGATGAATACACCATGCTCAGGCATGAAAGAGAGGAGGAAAAACGACGATTTAGGGACCATAAAAAATTTCATGAGCAGCAAAGCACAGACCAAGAGAGCATCACGAATTCAAGGCCTAGCCCTGCTCGACCAATTGGTACGAAGAAGGCAGCTGGACCTCGTGCCAATGGAGCTGTGAACGGAACTCCTAGCAGCAGAAGGTTATCGCTTAATCAGAATGGATCTAGATCTATGAATAAAGATGGAAAAAGGGATGGTATGAGACCAATGACACCTGTGAAGTATGATGCAATCTCGAAAGAGGACTCTGGCTCGCACGTCTCAGGTACGGAGCCTGTTTCATCTTCACCATGA

Protein sequence

MAVEDTQNPLQGETTCGTLLQKLQEIWDEVGESDEDRDKMLLQIDQECLDVYKRKVELADKSRAKLLQSLSDAKHELSNLLSALGEKSFSEFPESASGTIKEQLAAIAPALEQLWKQKEKRVKEFFDVQSEIQKICGEITGSLNISENPVVDESQLSVEKLDEYHCQLQELQKEKSERLHKVLEFVSTVHDLCAVLGIDFFGTVTEVHPSLNDATGVQSKSISNDTLSKLAKMVLALKEDKKQRLHKLQELATQLVDLWNLMDTPSEERSLFDHVTCNISASVDEVTVPGALALDLIEQAEVEVERLDQLKASRMKEIAFKKQSELEEIFAHAHIEIDPEAAREKIMTLIDSGNVEPSELLDDMDNKIAKAKEEVLSRKEILDKVEKWMLACEEESWLEDYNRDDNRYNASRGAHLNLKRAEKARILVNKIPALVDTLIAKTRAWEEDRGISFIYDGVPLLAMLDEYTMLRHEREEEKRRFRDHKKFHEQQSTDQESITNSRPSPARPIGTKKAAGPRANGAVNGTPSSRRLSLNQNGSRSMNKDGKRDGMRPMTPVKYDAISKEDSGSHVSGTEPVSSSP
BLAST of Carg25350-RA vs. NCBI nr
Match: XP_023005347.1 (65-kDa microtubule-associated protein 1-like [Cucurbita maxima])

HSP 1 Score: 1088.9 bits (2815), Expect = 0.0e+00
Identity = 575/583 (98.63%), Postives = 577/583 (98.97%), Query Frame = 0

Query: 1   MAVEDTQNPLQGETTCGTLLQKLQEIWDEVGESDEDRDKMLLQIDQECLDVYKRKVELAD 60
           MAVEDT+N LQGETTCGTLLQKLQEIWDEVGESDEDRDKMLLQIDQECLDVYKRKVE AD
Sbjct: 1   MAVEDTKNSLQGETTCGTLLQKLQEIWDEVGESDEDRDKMLLQIDQECLDVYKRKVEQAD 60

Query: 61  KSRAKLLQSLSDAKHELSNLLSALGEKSFSEFPESASGTIKEQLAAIAPALEQLWKQKEK 120
           KSRAKLLQSLSDAKHELSNLLSALGEKSFSEFPESASGTIKEQLAAIAPALEQLWKQKEK
Sbjct: 61  KSRAKLLQSLSDAKHELSNLLSALGEKSFSEFPESASGTIKEQLAAIAPALEQLWKQKEK 120

Query: 121 RVKEFFDVQSEIQKICGEITGSLNISENPVVDESQLSVEKLDEYHCQLQELQKEKSERLH 180
           RVKEFFDVQSEIQKICGEITGSLNISENPVVDESQLSVEKLDEYHCQLQELQKEKSERLH
Sbjct: 121 RVKEFFDVQSEIQKICGEITGSLNISENPVVDESQLSVEKLDEYHCQLQELQKEKSERLH 180

Query: 181 KVLEFVSTVHDLCAVLGIDFFGTVTEVHPSLNDATGVQSKSISNDTLSKLAKMVLALKED 240
           KVLEFVSTVHDLCAVLGIDFFGTVTEVHPSLNDATGVQSKSISNDTLSKLAKMVLALKED
Sbjct: 181 KVLEFVSTVHDLCAVLGIDFFGTVTEVHPSLNDATGVQSKSISNDTLSKLAKMVLALKED 240

Query: 241 KKQRLHKLQELATQLVDLWNLMDTPSEERSLFDHVTCNISASVDEVTVPGALALDLIEQA 300
           KKQRLHKLQELATQL+DLWNLMDTPSEERSLFDHVTCNISASVDEVTVPGALALDLIEQA
Sbjct: 241 KKQRLHKLQELATQLIDLWNLMDTPSEERSLFDHVTCNISASVDEVTVPGALALDLIEQA 300

Query: 301 EVEVERLDQLKASRMKEIAFKKQSELEEIFAHAHIEIDPEAAREKIMTLIDSGNVEPSEL 360
           EVEVERLDQLKASRMKEIAFKKQSELEEIFAHAHIEI PEAAREKIMTLIDSGNVEPSEL
Sbjct: 301 EVEVERLDQLKASRMKEIAFKKQSELEEIFAHAHIEIVPEAAREKIMTLIDSGNVEPSEL 360

Query: 361 LDDMDNKIAKAKEEVLSRKEILDKVEKWMLACEEESWLEDYNRDDNRYNASRGAHLNLKR 420
           LDDMDNKI KAKEEVLSRKEILDKVEKWMLACEEESWLEDYNRDDNRYNASRGAHLNLKR
Sbjct: 361 LDDMDNKIVKAKEEVLSRKEILDKVEKWMLACEEESWLEDYNRDDNRYNASRGAHLNLKR 420

Query: 421 AEKARILVNKIPALVDTLIAKTRAWEEDRGISFIYDGVPLLAMLDEYTMLRHEREEEKRR 480
           AEKARILVNKIPALVDTLIAKTRAWEEDRGISFIYDGVPLLAMLDEYTMLRHEREEEKRR
Sbjct: 421 AEKARILVNKIPALVDTLIAKTRAWEEDRGISFIYDGVPLLAMLDEYTMLRHEREEEKRR 480

Query: 481 FRDHKKFHEQQSTDQESITNSRPSPARPIGTKKAAGPRANGAVNGTPSSRRLSLNQNGSR 540
           FRDHKKFHEQQSTDQESITNSRPSPARPIGTKKAAGPRANGAVNGTPSSRRLSLNQNGSR
Sbjct: 481 FRDHKKFHEQQSTDQESITNSRPSPARPIGTKKAAGPRANGAVNGTPSSRRLSLNQNGSR 540

Query: 541 SMNKDGKRDGMRPMTPVKYDAISKED--SGSHVSGTEPVSSSP 582
           SMNKDGKRDGMRPMTPVKYDAISKED  SGSHVSGTEPVSSSP
Sbjct: 541 SMNKDGKRDGMRPMTPVKYDAISKEDSGSGSHVSGTEPVSSSP 583

BLAST of Carg25350-RA vs. NCBI nr
Match: XP_022942085.1 (65-kDa microtubule-associated protein 1-like [Cucurbita moschata])

HSP 1 Score: 1088.2 bits (2813), Expect = 0.0e+00
Identity = 571/579 (98.62%), Postives = 574/579 (99.14%), Query Frame = 0

Query: 1   MAVEDTQNPLQGETTCGTLLQKLQEIWDEVGESDEDRDKMLLQIDQECLDVYKRKVELAD 60
           MAVEDTQNPLQGETTCGTLLQKLQEIWDEVGESDEDRDKMLLQIDQECLDVYKRKVELAD
Sbjct: 1   MAVEDTQNPLQGETTCGTLLQKLQEIWDEVGESDEDRDKMLLQIDQECLDVYKRKVELAD 60

Query: 61  KSRAKLLQSLSDAKHELSNLLSALGEKSFSEFPESASGTIKEQLAAIAPALEQLWKQKEK 120
           KSRAKLLQSLSDAKHELSNLLSALGEKSFSEFPESASGTIKEQLAAIAPALEQLWKQKEK
Sbjct: 61  KSRAKLLQSLSDAKHELSNLLSALGEKSFSEFPESASGTIKEQLAAIAPALEQLWKQKEK 120

Query: 121 RVKEFFDVQSEIQKICGEITGSLNISENPVVDESQLSVEKLDEYHCQLQELQKEKSERLH 180
           RVKEFFDVQSEIQKICGEITGSLNISENPVVDESQLSVEKLDEYHCQLQELQKEKSERLH
Sbjct: 121 RVKEFFDVQSEIQKICGEITGSLNISENPVVDESQLSVEKLDEYHCQLQELQKEKSERLH 180

Query: 181 KVLEFVSTVHDLCAVLGIDFFGTVTEVHPSLNDATGVQSKSISNDTLSKLAKMVLALKED 240
           KVLEFVSTVHDLCAVLGIDFFGTVTEVHPSLNDATGVQSKSISNDTLSKLAKMVLALKED
Sbjct: 181 KVLEFVSTVHDLCAVLGIDFFGTVTEVHPSLNDATGVQSKSISNDTLSKLAKMVLALKED 240

Query: 241 KKQRLHKLQELATQLVDLWNLMDTPSEERSLFDHVTCNISASVDEVTVPGALALDLIEQA 300
           K+QRLHKLQELATQL+DLWNLMDTPSEERSLFDHVTCNISASVDEVTVPGALALDLIEQA
Sbjct: 241 KRQRLHKLQELATQLIDLWNLMDTPSEERSLFDHVTCNISASVDEVTVPGALALDLIEQA 300

Query: 301 EVEVERLDQLKASRMKEIAFKKQSELEEIFAHAHIEIDPEAAREKIMTLIDSGNVEPSEL 360
           EVEVERLDQLKASRMKEIAFKKQSELEEIFAHAHIEIDPEAAREKIMTLIDSGNVEPSEL
Sbjct: 301 EVEVERLDQLKASRMKEIAFKKQSELEEIFAHAHIEIDPEAAREKIMTLIDSGNVEPSEL 360

Query: 361 LDDMDNKIAKAKEEVLSRKEILDKVEKWMLACEEESWLEDYNRDDNRYNASRGAHLNLKR 420
           LDDMDNKIAKAKEEVLSRKEILDKVEKWMLACEEESWLEDYNRDDNRYNASRGAHLNLKR
Sbjct: 361 LDDMDNKIAKAKEEVLSRKEILDKVEKWMLACEEESWLEDYNRDDNRYNASRGAHLNLKR 420

Query: 421 AEKARILVNKIPALVDTLIAKTRAWEEDRGISFIYDGVPLLAMLDEYTMLRHEREEEKRR 480
           AEKARILVNKIPALVDTLIAKTRAWEEDRGISFIYDGVPLLAMLDEYTMLRHEREEEKRR
Sbjct: 421 AEKARILVNKIPALVDTLIAKTRAWEEDRGISFIYDGVPLLAMLDEYTMLRHEREEEKRR 480

Query: 481 FRDHKKFHEQQSTDQESITNSRPSPARPIGTKKAAGPRANGAVNGTPSSRRLSLNQNGSR 540
           FRDHKKFHEQQSTDQESITNSRPSPARPIGTKKAAGPRANGAVNGTPSSRRLSLNQNGSR
Sbjct: 481 FRDHKKFHEQQSTDQESITNSRPSPARPIGTKKAAGPRANGAVNGTPSSRRLSLNQNGSR 540

Query: 541 SMNKDGKRDGMRPMTPVKYDAISKEDSGSHVSGTEPVSS 580
           SMNKDGKRDGMRPMTPVKYDAISKEDSGSHVS    V +
Sbjct: 541 SMNKDGKRDGMRPMTPVKYDAISKEDSGSHVSAAGVVDT 579

BLAST of Carg25350-RA vs. NCBI nr
Match: XP_023520760.1 (65-kDa microtubule-associated protein 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1085.1 bits (2805), Expect = 0.0e+00
Identity = 569/572 (99.48%), Postives = 570/572 (99.65%), Query Frame = 0

Query: 1   MAVEDTQNPLQGETTCGTLLQKLQEIWDEVGESDEDRDKMLLQIDQECLDVYKRKVELAD 60
           MAVEDTQNPLQGETTCGTLLQKLQEIWDEVGESDEDRDKMLLQIDQECLDVYKRKVELAD
Sbjct: 1   MAVEDTQNPLQGETTCGTLLQKLQEIWDEVGESDEDRDKMLLQIDQECLDVYKRKVELAD 60

Query: 61  KSRAKLLQSLSDAKHELSNLLSALGEKSFSEFPESASGTIKEQLAAIAPALEQLWKQKEK 120
           KSRAKLLQSLSDAKHELSNLLSALGEKSFSEFPESASGTIKEQLAAIAPALEQLWKQKEK
Sbjct: 61  KSRAKLLQSLSDAKHELSNLLSALGEKSFSEFPESASGTIKEQLAAIAPALEQLWKQKEK 120

Query: 121 RVKEFFDVQSEIQKICGEITGSLNISENPVVDESQLSVEKLDEYHCQLQELQKEKSERLH 180
           RVKEFFDVQSEIQKICGEITGSLNISENPVVDESQLSVEKLDEYHCQLQELQKEKSERLH
Sbjct: 121 RVKEFFDVQSEIQKICGEITGSLNISENPVVDESQLSVEKLDEYHCQLQELQKEKSERLH 180

Query: 181 KVLEFVSTVHDLCAVLGIDFFGTVTEVHPSLNDATGVQSKSISNDTLSKLAKMVLALKED 240
           KVLEFVSTVHDLCAVLGIDFFGTVTEVHPSLNDATGVQSKSISNDTLSKLAKMVLALKED
Sbjct: 181 KVLEFVSTVHDLCAVLGIDFFGTVTEVHPSLNDATGVQSKSISNDTLSKLAKMVLALKED 240

Query: 241 KKQRLHKLQELATQLVDLWNLMDTPSEERSLFDHVTCNISASVDEVTVPGALALDLIEQA 300
           KKQRLHKLQELATQL+DLWNLMDTPSEERSLFDHVTCNISASVDEVTVPGALALDLIEQA
Sbjct: 241 KKQRLHKLQELATQLIDLWNLMDTPSEERSLFDHVTCNISASVDEVTVPGALALDLIEQA 300

Query: 301 EVEVERLDQLKASRMKEIAFKKQSELEEIFAHAHIEIDPEAAREKIMTLIDSGNVEPSEL 360
            VEVERLDQLKASRMKEIAFKKQSELEEIFAHAHIEIDPEAAREKIMTLIDSGNVEPSEL
Sbjct: 301 AVEVERLDQLKASRMKEIAFKKQSELEEIFAHAHIEIDPEAAREKIMTLIDSGNVEPSEL 360

Query: 361 LDDMDNKIAKAKEEVLSRKEILDKVEKWMLACEEESWLEDYNRDDNRYNASRGAHLNLKR 420
           LDDMDNKIAKAKEEVLSRKEILDKVEKWMLACEEESWLEDYNRDDNRYNASRGAHLNLKR
Sbjct: 361 LDDMDNKIAKAKEEVLSRKEILDKVEKWMLACEEESWLEDYNRDDNRYNASRGAHLNLKR 420

Query: 421 AEKARILVNKIPALVDTLIAKTRAWEEDRGISFIYDGVPLLAMLDEYTMLRHEREEEKRR 480
           AEKARILVNKIPALVDTLIAKTRAWEEDRGISFIYDGVPLLAMLDEYTMLRHEREEEKRR
Sbjct: 421 AEKARILVNKIPALVDTLIAKTRAWEEDRGISFIYDGVPLLAMLDEYTMLRHEREEEKRR 480

Query: 481 FRDHKKFHEQQSTDQESITNSRPSPARPIGTKKAAGPRANGAVNGTPSSRRLSLNQNGSR 540
           FRDHKKFHEQQSTDQESITNSRPSPARPI TKKAAGPRANGAVNGTPSSRRLSLNQNGSR
Sbjct: 481 FRDHKKFHEQQSTDQESITNSRPSPARPISTKKAAGPRANGAVNGTPSSRRLSLNQNGSR 540

Query: 541 SMNKDGKRDGMRPMTPVKYDAISKEDSGSHVS 573
           SMNKDGKRDGMRPMTPVKYDAISKEDSGSHVS
Sbjct: 541 SMNKDGKRDGMRPMTPVKYDAISKEDSGSHVS 572

BLAST of Carg25350-RA vs. NCBI nr
Match: XP_011650516.1 (PREDICTED: 65-kDa microtubule-associated protein 1 [Cucumis sativus] >KGN56090.1 hypothetical protein Csa_3G073820 [Cucumis sativus])

HSP 1 Score: 1045.8 bits (2703), Expect = 5.4e-302
Identity = 546/581 (93.98%), Postives = 563/581 (96.90%), Query Frame = 0

Query: 1   MAVEDTQNPLQGETTCGTLLQKLQEIWDEVGESDEDRDKMLLQIDQECLDVYKRKVELAD 60
           MAV DTQ PLQGETTCGTLLQKLQEIWDEVGE+DE+RDKMLLQIDQECLDVYKRKVELAD
Sbjct: 1   MAVADTQIPLQGETTCGTLLQKLQEIWDEVGETDEERDKMLLQIDQECLDVYKRKVELAD 60

Query: 61  KSRAKLLQSLSDAKHELSNLLSALGEKSFSEFPESASGTIKEQLAAIAPALEQLWKQKEK 120
           KSRAKLLQSLSDAKHELS+LLSALGEKSFSEFPE ASGTIKEQLAAIAPALE+LWKQKE+
Sbjct: 61  KSRAKLLQSLSDAKHELSSLLSALGEKSFSEFPEKASGTIKEQLAAIAPALEKLWKQKEQ 120

Query: 121 RVKEFFDVQSEIQKICGEITGSLNISENPVVDESQLSVEKLDEYHCQLQELQKEKSERLH 180
           RVKEFFDVQSEIQKICGEI GSLN+SENPVVDESQLSVEKLDEYHCQLQELQKEKS+RLH
Sbjct: 121 RVKEFFDVQSEIQKICGEIAGSLNLSENPVVDESQLSVEKLDEYHCQLQELQKEKSDRLH 180

Query: 181 KVLEFVSTVHDLCAVLGIDFFGTVTEVHPSLNDATGVQSKSISNDTLSKLAKMVLALKED 240
           KVLEFVSTVHDLCAVLGIDFF TVTEVHPSLNDATGVQSKSISNDTLSKLAKMVLALKED
Sbjct: 181 KVLEFVSTVHDLCAVLGIDFFATVTEVHPSLNDATGVQSKSISNDTLSKLAKMVLALKED 240

Query: 241 KKQRLHKLQELATQLVDLWNLMDTPSEERSLFDHVTCNISASVDEVTVPGALALDLIEQA 300
           KKQRLHKLQELATQL+DLWNLMDTPSEERSLFDHVTCNISASVDEVTVPGALALDLIEQA
Sbjct: 241 KKQRLHKLQELATQLIDLWNLMDTPSEERSLFDHVTCNISASVDEVTVPGALALDLIEQA 300

Query: 301 EVEVERLDQLKASRMKEIAFKKQSELEEIFAHAHIEIDPEAAREKIMTLIDSGNVEPSEL 360
           EVEVERLDQLKASRMKEIAFKKQSELEEIF+HAHIEIDPEAAREKIM LIDSGNVEP+EL
Sbjct: 301 EVEVERLDQLKASRMKEIAFKKQSELEEIFSHAHIEIDPEAAREKIMALIDSGNVEPAEL 360

Query: 361 LDDMDNKIAKAKEEVLSRKEILDKVEKWMLACEEESWLEDYNRDDNRYNASRGAHLNLKR 420
           LDDMD++I KAKEEVLSRKEILDKVEKWMLACEEESWLEDYNRDDNRYNASRGAHLNLKR
Sbjct: 361 LDDMDDQITKAKEEVLSRKEILDKVEKWMLACEEESWLEDYNRDDNRYNASRGAHLNLKR 420

Query: 421 AEKARILVNKIPALVDTLIAKTRAWEEDRGISFIYDGVPLLAMLDEYTMLRHEREEEKRR 480
           AEKARILVNKIPALVDTLIAKTRAWEEDRGISFIYDGVPLLAMLDEYTMLRHEREEEKRR
Sbjct: 421 AEKARILVNKIPALVDTLIAKTRAWEEDRGISFIYDGVPLLAMLDEYTMLRHEREEEKRR 480

Query: 481 FRDHKKFHEQQSTDQESITNSRPSPARPIGTKKAAGPRANGAVNGTPSSRRLSLNQNGSR 540
            RDHKKFH+QQ+TD E ITNSRPSP R I TKKA G RANGA NGTPSSRRLSLNQNGSR
Sbjct: 481 LRDHKKFHDQQTTDPEIITNSRPSPVRTIATKKAGGARANGAANGTPSSRRLSLNQNGSR 540

Query: 541 SMNKDGKRDGMRPMTPVKYDAISKEDSGSHVSGTEPVSSSP 582
           SMNKD KRD MRPMTPVKYDAISK+DSGSH+SGTEPVS+SP
Sbjct: 541 SMNKDSKRDSMRPMTPVKYDAISKDDSGSHISGTEPVSASP 581

BLAST of Carg25350-RA vs. NCBI nr
Match: XP_022932234.1 (65-kDa microtubule-associated protein 1-like [Cucurbita moschata])

HSP 1 Score: 1045.0 bits (2701), Expect = 9.2e-302
Identity = 545/581 (93.80%), Postives = 564/581 (97.07%), Query Frame = 0

Query: 1   MAVEDTQNPLQGETTCGTLLQKLQEIWDEVGESDEDRDKMLLQIDQECLDVYKRKVELAD 60
           MAV D QNPLQGETTCGTLLQKLQEIWDEVGESD++RDKMLLQIDQECLDVYKRKVE AD
Sbjct: 1   MAVADIQNPLQGETTCGTLLQKLQEIWDEVGESDKERDKMLLQIDQECLDVYKRKVEQAD 60

Query: 61  KSRAKLLQSLSDAKHELSNLLSALGEKSFSEFPESASGTIKEQLAAIAPALEQLWKQKEK 120
           KSRAKLLQSLSDAK ELS+LLSALGEKSFSEFPE ASGTIKEQLAAIAPALEQLWKQKE+
Sbjct: 61  KSRAKLLQSLSDAKLELSSLLSALGEKSFSEFPEKASGTIKEQLAAIAPALEQLWKQKEQ 120

Query: 121 RVKEFFDVQSEIQKICGEITGSLNISENPVVDESQLSVEKLDEYHCQLQELQKEKSERLH 180
           RVKEFFDVQSEIQKICGEI+GSLN+SENPVVDES LSVEKLDEYHCQLQELQKEKS+RLH
Sbjct: 121 RVKEFFDVQSEIQKICGEISGSLNLSENPVVDESHLSVEKLDEYHCQLQELQKEKSDRLH 180

Query: 181 KVLEFVSTVHDLCAVLGIDFFGTVTEVHPSLNDATGVQSKSISNDTLSKLAKMVLALKED 240
           KVLEFVSTVHDLCAVLGIDFF TVTEVHPSLNDATGVQSKSISNDTLSKLAKMVLALKED
Sbjct: 181 KVLEFVSTVHDLCAVLGIDFFATVTEVHPSLNDATGVQSKSISNDTLSKLAKMVLALKED 240

Query: 241 KKQRLHKLQELATQLVDLWNLMDTPSEERSLFDHVTCNISASVDEVTVPGALALDLIEQA 300
           KKQRLHKLQELATQL+DLWNLMDTPSEERSLFDHVTCNISASVDEVTVPGALALDLIEQA
Sbjct: 241 KKQRLHKLQELATQLIDLWNLMDTPSEERSLFDHVTCNISASVDEVTVPGALALDLIEQA 300

Query: 301 EVEVERLDQLKASRMKEIAFKKQSELEEIFAHAHIEIDPEAAREKIMTLIDSGNVEPSEL 360
           EVEVERLDQLKAS+MKEIAFKKQSELEEIF+HAHIE+DP+AAR+KIM LIDSGNVEP+EL
Sbjct: 301 EVEVERLDQLKASKMKEIAFKKQSELEEIFSHAHIEVDPDAARDKIMALIDSGNVEPAEL 360

Query: 361 LDDMDNKIAKAKEEVLSRKEILDKVEKWMLACEEESWLEDYNRDDNRYNASRGAHLNLKR 420
           LDDMD++IAKAKEEVLSRKEILDKVEKWMLACEEESWLEDYNRDDNRYNASRGAHLNLKR
Sbjct: 361 LDDMDDQIAKAKEEVLSRKEILDKVEKWMLACEEESWLEDYNRDDNRYNASRGAHLNLKR 420

Query: 421 AEKARILVNKIPALVDTLIAKTRAWEEDRGISFIYDGVPLLAMLDEYTMLRHEREEEKRR 480
           AEKARILVNKIPALVDTLIAKTRAWEEDRGISFIYDGVPLLAMLDEYTMLRHEREEEKRR
Sbjct: 421 AEKARILVNKIPALVDTLIAKTRAWEEDRGISFIYDGVPLLAMLDEYTMLRHEREEEKRR 480

Query: 481 FRDHKKFHEQQSTDQESITNSRPSPARPIGTKKAAGPRANGAVNGTPSSRRLSLNQNGSR 540
            RD KKFHEQQSTDQE+I N RPSP RP+GTKKAAGPRANGA NGTPSSRRLSLNQNGSR
Sbjct: 481 SRDQKKFHEQQSTDQETINNPRPSPVRPVGTKKAAGPRANGAANGTPSSRRLSLNQNGSR 540

Query: 541 SMNKDGKRDGMRPMTPVKYDAISKEDSGSHVSGTEPVSSSP 582
           SMNKDG+RD MRPMTPVKYDAISKEDS SH SGTEPVSSSP
Sbjct: 541 SMNKDGRRDSMRPMTPVKYDAISKEDSASHFSGTEPVSSSP 581

BLAST of Carg25350-RA vs. TAIR10
Match: AT5G55230.2 (microtubule-associated proteins 65-1)

HSP 1 Score: 741.5 bits (1913), Expect = 4.0e-214
Identity = 418/618 (67.64%), Postives = 484/618 (78.32%), Query Frame = 0

Query: 1   MAVEDTQNPLQGETTCGTLLQKLQEIWDEVGESDEDRDKMLLQIDQECLDVYKRKVELAD 60
           MAV DT++P  GE TCGTLL+KLQEIWDEVGESD++RDK+LLQI+QECLDVYKRKVE A 
Sbjct: 1   MAVTDTESPHLGEITCGTLLEKLQEIWDEVGESDDERDKLLLQIEQECLDVYKRKVEQAA 60

Query: 61  KSRAKLLQSLSDAKHELSNLLSALGEKSFSEFPESASGTIKEQLAAIAPALEQLWKQKEK 120
           KSRA+LLQ+LSDA  ELS+L  +LG+KS    P+ +SGTIKEQLAAIAPALEQLW+QKE+
Sbjct: 61  KSRAELLQTLSDANAELSSLTMSLGDKSLVGIPDKSSGTIKEQLAAIAPALEQLWQQKEE 120

Query: 121 RVKEFFDVQSEIQKICGEITGSLNISENPVVDESQLSVEKLDEYHCQLQELQKEKSERLH 180
           RV+EF DVQS+IQKICG+I G L+ +E P+VDES LS++KLD++  QLQELQKEKS+RL 
Sbjct: 121 RVREFSDVQSQIQKICGDIAGGLS-NEVPIVDESDLSLKKLDDFQSQLQELQKEKSDRLR 180

Query: 181 KVLEFVSTVHDLCAVLGIDFFGTVTEVHPSLNDATGVQSKSISNDTLSKLAKMVLALKED 240
           KVLEFVSTVHDLCAVLG+DF  TVTEVHPSL++ T VQSKSISN+TLS+LAK VL LK+D
Sbjct: 181 KVLEFVSTVHDLCAVLGLDFLSTVTEVHPSLDEDTSVQSKSISNETLSRLAKTVLTLKDD 240

Query: 241 KKQRLHKLQELATQLVDLWNLMDTPSEERSLFDHVTCNISASVDEVTVPGALALDLIEQ- 300
           K           TQL+DLWNLMDTP EER LFDHVTCNIS+SVDEVTVPGALA DLIEQ 
Sbjct: 241 KXXXXXXXXXXXTQLIDLWNLMDTPDEERELFDHVTCNISSSVDEVTVPGALARDLIEQV 300

Query: 301 ----------------------------AEVEVERLDQLKASRMKEIAFKKQSELEEIFA 360
                                       AEVEV+RLDQLKASRMKEIAFKKQSELEEI+A
Sbjct: 301 IYIALINLPMSSLRNQLLLANIHVKFVKAEVEVDRLDQLKASRMKEIAFKKQSELEEIYA 360

Query: 361 HAHIEIDPEAAREKIMTLIDSGNVEPSELLDDMDNKIAKAKEEVLSRKEILDKVEKWMLA 420
            AH+E++PE+ARE+IM+LIDSGNVEP+ELL DMD++I+KAKEE  SRK+ILD+VEKWM A
Sbjct: 361 RAHVEVNPESARERIMSLIDSGNVEPTELLADMDSQISKAKEEAFSRKDILDRVEKWMSA 420

Query: 421 CEEESWLEDYNRDDNRYNASRGAHLNLKRAEKARILVNKIPALVDTLIAKTRAWEEDRGI 480
           CEEESWLEDYNRD NRY+ASRGAHLNLKRAEKARILV+KIPA+VDTL+AKTRAWEE+  +
Sbjct: 421 CEEESWLEDYNRDQNRYSASRGAHLNLKRAEKARILVSKIPAMVDTLVAKTRAWEEEHSM 480

Query: 481 SFIYDGVPLLAMLDEYTMLRHEREEEKRRFRDHKKFHEQQSTDQESITNSRPSPARPIGT 540
           SF YDGVPLLAMLDEY MLR EREEE                  ES  ++RPSPARP+  
Sbjct: 481 SFAYDGVPLLAMLDEYGMLRQEREEEXXXXXXXXXXXXXXXXXXESAFSTRPSPARPVSA 540

Query: 541 KKAAGPRA-NGAVNGTPSSRRLSL--NQNGSRSMNKD-GKRDGM-RPMTPVKYDAISKED 582
           KK  GPRA NG  NGT  +RRLSL  NQNGSRS  K+ G+R+ + RP  P  Y AISKE+
Sbjct: 541 KKTVGPRANNGGANGT-HNRRLSLNANQNGSRSTAKEAGRRETLNRPAAPTNYVAISKEE 600

BLAST of Carg25350-RA vs. TAIR10
Match: AT4G26760.1 (microtubule-associated protein 65-2)

HSP 1 Score: 678.7 bits (1750), Expect = 3.2e-195
Identity = 385/585 (65.81%), Postives = 448/585 (76.58%), Query Frame = 0

Query: 1   MAVEDTQNPLQGETTCGTLLQKLQEIWDEVGESDEDRDKMLLQIDQECLDVYKRKVELAD 60
           MAV + +NPL GE TCGTLLQKLQEIWDEVGESDE+RDK+LLQI++ECL+VYK+KVELA 
Sbjct: 1   MAVTEAENPLLGEITCGTLLQKLQEIWDEVGESDEERDKLLLQIEEECLNVYKKKVELAA 60

Query: 61  KSRAKLLQSLSDAKHELSNLLSALGEKSFSEFPESASGTIKEQLAAIAPALEQLWKQKEK 120
           KSRA+LLQ+LSDA  ELSNL +ALGEKS+ + P+  SGTIKEQL+AIAPALEQLW+QKE+
Sbjct: 61  KSRAELLQTLSDATVELSNLTTALGEKSYIDIPDKTSGTIKEQLSAIAPALEQLWQQKEE 120

Query: 121 RVKEFFDVQSEIQKICGEITGSLNISENPVVDESQLSVEKLDEYHCQLQELQKEKSERLH 180
           RV+ F DVQS+IQKIC EI G LN   + VVDE+ LS+++LD++  +LQELQKEKS+RL 
Sbjct: 121 RVRAFSDVQSQIQKICEEIAGGLNNGPH-VVDETDLSLKRLDDFQRKLQELQKEKSDRLQ 180

Query: 181 KVLEFVSTVHDLCAVLGIDFFGTVTEVHPSLNDATGVQSKSISNDTLSKLAKMVLALKED 240
           KVLEFVSTVHDLCAVL +DF  TVTEVHPSL++A GVQ+KSISN+TL++L          
Sbjct: 181 KVLEFVSTVHDLCAVLRLDFLSTVTEVHPSLDEANGVQTKSISNETLARLXXXXXXXXXX 240

Query: 241 KKQRLHKLQELATQLVDLWNLMDTPSEERSLFDHVTCNISASVDEVTVPGALALDLIEQA 300
                                     EER LFDHVT NISASV EVT  GALALDLIEQA
Sbjct: 241 XXXXXXXXXXXXXXXXXXXXXXXXXXEERELFDHVTSNISASVHEVTASGALALDLIEQA 300

Query: 301 EVEVERLDQLKASRMKEIAFKKQSELEEIFAHAHIEIDPEAAREKIMTLIDSGNVEPSEL 360
           EVEV+RLDQLK+SRMKEIAFKKQSELEEI+A AHIEI PE  RE+IM+LID+GN EP+EL
Sbjct: 301 EVEVDRLDQLKSSRMKEIAFKKQSELEEIYARAHIEIKPEVVRERIMSLIDAGNTEPTEL 360

Query: 361 LDDMDNKIAKAKEEVLSRKEILDKVEKWMLACEEESWLEDYNRDDNRYNASRGAHLNLKR 420
           L DMD++IAKAKEE  SRKEILD+VEKWM ACEEESWLEDYNRD NRY+ASRGAHLNLKR
Sbjct: 361 LADMDSQIAKAKEEAFSRKEILDRVEKWMSACEEESWLEDYNRDQNRYSASRGAHLNLKR 420

Query: 421 AEKARILVNKIPALVDTLIAKTRAWEEDRGISFIYDGVPLLAMLDEYTMLRHEREEEKRR 480
           AEKARILV+KI A+VDTLIAKTRAWEE+  +SF YDGVPLLAMLDEYTMLR ERE     
Sbjct: 421 AEKARILVSKITAMVDTLIAKTRAWEEENSMSFEYDGVPLLAMLDEYTMLRQEREXXXXX 480

Query: 481 FRDHKKFHEQQSTDQESITNSRPSPARPIGTKKAAGPRAN-GAVNGTPSSRRLSL--NQN 540
                       TDQES   S+PSPARP+  KK  G R N G +N TP  RRLS+  NQN
Sbjct: 481 XXXXXXXXXXPHTDQESAFGSKPSPARPVSAKKPVGTRVNGGGLNETP-MRRLSMNSNQN 540

Query: 541 GSRSMNKDGKRDGMRPM-TPVKYDAISKEDSGSHVSGTEPVSSSP 582
           GS+S     KRD +  + +P    A +K+D+ S VS  +PV +SP
Sbjct: 541 GSKS-----KRDSLNKIASPSNIVANTKDDAASPVSRADPVMASP 578

BLAST of Carg25350-RA vs. TAIR10
Match: AT5G51600.1 (Microtubule associated protein (MAP65/ASE1) family protein)

HSP 1 Score: 469.2 bits (1206), Expect = 3.8e-132
Identity = 247/502 (49.20%), Postives = 363/502 (72.31%), Query Frame = 0

Query: 10  LQGETTCGTLLQKLQEIWDEVGESDEDRDKMLLQIDQECLDVYKRKVELADKSRAKLLQS 69
           LQ ETTCG+LL +LQ IWDEVGE++ DRD+MLL++++ECL+VY+RKV+ A++ RA+L Q+
Sbjct: 10  LQVETTCGSLLFELQIIWDEVGETETDRDQMLLELERECLEVYRRKVDQANRCRAQLRQA 69

Query: 70  LSDAKHELSNLLSALGEKSFS-EFPESASGTIKEQLAAIAPALEQLWKQKEKRVKEFFDV 129
           ++DA+ +L+ + SA+GE+       + + G++K++L  I P LE++ K+K +R  +F  V
Sbjct: 70  IADAEAQLAAICSAMGERPVHIRQSDQSVGSLKQELGRILPELEEMQKRKVERRNQFIVV 129

Query: 130 QSEIQKICGEITGSLN-ISENPVVDESQLSVEKLDEYHCQLQELQKEKSERLHKVLEFVS 189
             +I  I  +I G    +   P++DE+ LS+ KL+E HCQLQ LQKEK +R+  + + + 
Sbjct: 130 MEQIDSITNDIKGQGELVHSEPLIDETNLSMRKLEELHCQLQVLQKEKIDRVETIRKHLC 189

Query: 190 TVHDLCAVLGIDFFGTVTEVHPSLNDATGVQSKSISNDTLSKLAKMVLALKEDKKQRLHK 249
           T++  C+VLG+DF   V +V+P+L+D  G   +S+S+ T+ KL   V  L E K QR+ +
Sbjct: 190 TLYSHCSVLGMDFNEVVGQVNPTLSDPEG--PRSLSDHTIEKLGAAVQKLMEVKIQRMQR 249

Query: 250 LQELATQLVDLWNLMDTPSEERSLFDHVTCNISASVDEVTVPGALALDLIEQAEVEVERL 309
           LQ+LAT +++LWNLMDTP EE+  + H+TCNI+AS  E+T   +L+ D I+  E EV RL
Sbjct: 250 LQDLATTMLELWNLMDTPIEEQQEYQHITCNIAASEHEITEANSLSEDFIKYVEAEVVRL 309

Query: 310 DQLKASRMKEIAFKKQSELEEIFAHAHIEIDPEAAREKIMTLIDSGNVEPSELLDDMDNK 369
           D++KAS+MKE+  KK+SELEEI    H+    ++A ++ +  I+SG V+ + +L+ ++  
Sbjct: 310 DEVKASKMKELVLKKRSELEEICRKTHLLPVSDSAIDQTIVAIESGIVDATMVLEHLEQH 369

Query: 370 IAKAKEEVLSRKEILDKVEKWMLACEEESWLEDYNRDDNRYNASRGAHLNLKRAEKARIL 429
           I+K KEE LSRKEIL++VEKW+ AC+EESWLE+YNRDDNRYNA RGAHL LKRAEKAR L
Sbjct: 370 ISKIKEEALSRKEILERVEKWLSACDEESWLEEYNRDDNRYNAGRGAHLTLKRAEKARNL 429

Query: 430 VNKIPALVDTLIAKTRAWEEDRGISFIYDGVPLLAMLDEYTMLRHEREEEKRRFRDHKKF 489
           V K+P +V+ L +KT  WE++ GI F+YDG+ LL+ML+EY +LR EREEE RR RD KK 
Sbjct: 430 VTKLPGMVEALASKTIVWEQENGIEFLYDGIRLLSMLEEYNILRQEREEEHRRQRDQKKL 489

Query: 490 HEQQSTDQESITNSRPSPARPI 510
             Q   +QE++  S+PSP++P+
Sbjct: 490 QGQLIAEQEALYGSKPSPSKPL 509

BLAST of Carg25350-RA vs. TAIR10
Match: AT2G38720.1 (microtubule-associated protein 65-5)

HSP 1 Score: 434.5 bits (1116), Expect = 1.0e-121
Identity = 252/590 (42.71%), Postives = 373/590 (63.22%), Query Frame = 0

Query: 14  TTCGTLLQKLQEIWDEVGESDEDRDKMLLQIDQECLDVYKRKVELADKSRAKLLQSLSDA 73
           TTC +LL++LQ IWDE+GES  +RDKMLL+++QECLD+Y +KVE   K RA+L +SL+ A
Sbjct: 7   TTCTSLLEELQMIWDEIGESYNERDKMLLELEQECLDIYNKKVEKTRKFRAELQRSLAQA 66

Query: 74  KHELSNLLSALGEKSFSEFPESASGTIKEQLAAIAPALEQLWKQKEKRVKEFFDVQSEIQ 133
           + E+++L+SALGEK    F +   G++KEQ++++ P LE L  +K++R KE  +  ++I 
Sbjct: 67  EAEIASLMSALGEK--VSFAKKKEGSLKEQISSVKPVLEDLLMKKDRRRKELSETLNQIA 126

Query: 134 KICGEITGS-LNISENPVVDESQLSVEKLDEYHCQLQELQKEKSERLHKVLEFVSTVHDL 193
           +I   I G+   +S    VDES L+  KLDE    LQ+L+ EK+ RL KV  ++S VH+L
Sbjct: 127 EITSNIAGNDYTVSSGSEVDESDLTQRKLDELRADLQDLRNEKAVRLQKVNSYISAVHEL 186

Query: 194 CAVLGIDFFGTVTEVHPSLNDATGVQSKSISNDTLSKLAKMVLALKEDKKQRLHKLQELA 253
             +L  DF   +  VH SL + +   SKSISNDTL++  ++V +LK +K +RL KLQ L 
Sbjct: 187 SEILSFDFSKALNSVHSSLTEFSKTHSKSISNDTLARFTELVKSLKAEKHERLLKLQGLG 246

Query: 254 TQLVDLWNLMDTPSEERSLFDHVTCNISASVDEVTVPGALALDLIEQAEVEVERLDQLKA 313
             + +LWNLM+TP +ER  FDH +  +S+  D+    G L+LD+I +AE EV RL+ LK+
Sbjct: 247 RSMQELWNLMETPMDERRRFDHCSSLLSSLPDDALKKGCLSLDIIREAEDEVRRLNSLKS 306

Query: 314 SRMKEIAFKKQSELEEIFAHAHIEIDPEAAREKIMTLIDSGNVEPSELLDDMDNKIAKAK 373
           S+MKE+ FK+Q ELEEI    H++I+ +AAR+ ++ LI+SG+ + S++L  +D +I KA+
Sbjct: 307 SKMKELVFKRQCELEEICRGNHMDINSDAARKSLVELIESGDGDLSDILASIDGQIEKAR 366

Query: 374 EEVLSRKEILDKVEKWMLACEEESWLEDYNRDDNRYNASRGAHLNLKRAEKARILVNKIP 433
           EE LSRKEILDKV+KW  A EEE+WL+DY +D+NR++A RGAH NLKRAEKAR L++KIP
Sbjct: 367 EEALSRKEILDKVDKWRHAKEEETWLDDYEKDENRFSAVRGAHKNLKRAEKARSLISKIP 426

Query: 434 ------------------------------------ALVDTLIAKTRAWEEDRGISFIYD 493
                                               ++VD L  K +AWE++RG+ F+ D
Sbjct: 427 VFKQFNKKFWALLFSPKQLFKFSNSIIDYCENSTTHSMVDVLTTKVKAWEKERGVPFLCD 486

Query: 494 GVPLLAMLDEYTMLRHEREEEKRRFRDHKKFHEQQSTDQESITNSRPSPARPIGTKKAAG 553
             PLL  L++  ++R +REEEKR+FR+ K+   Q +T++E+   S+ +  +P+G      
Sbjct: 487 KQPLLQTLEDDIVIRAQREEEKRQFREQKRLQGQLATEKEAKYGSKSAKKKPLGQS---- 546

Query: 554 PRANGAVNGTPSSRRLSLNQNGSRSMNKDGKRDGMRPMTPVKYDAISKED 567
                 V  TP  RR  +     RS+   GK      + P+ Y A+ K+D
Sbjct: 547 -LNTDNVTKTPIGRR--IGNTPGRSVTSGGKDYRGNAVIPLNYVALQKDD 587

BLAST of Carg25350-RA vs. TAIR10
Match: AT2G01910.1 (Microtubule associated protein (MAP65/ASE1) family protein)

HSP 1 Score: 433.0 bits (1112), Expect = 3.0e-121
Identity = 257/592 (43.41%), Postives = 380/592 (64.19%), Query Frame = 0

Query: 14  TTCGTLLQKLQEIWDEVGESDEDRDKMLLQIDQECLDVYKRKVELADKSRAKLLQSLSDA 73
           TTC  LL++LQ+IW E+GE++ ++D+ML+++++ECL +Y+RKV+ A  S+AKL QS++  
Sbjct: 16  TTCNNLLRELQKIWVEIGETETEKDRMLMELERECLQIYQRKVDEAANSKAKLHQSVASI 75

Query: 74  KHELSNLLSALGEKSFSE--FPESASGTIKEQLAAIAPALEQLWKQKEKRVKEFFDVQSE 133
           + E+++L++ALG  + +     +  S ++KE+LAA+ P +E+L  QKE+R+K+F D++++
Sbjct: 76  EAEVASLMAALGVLNINSPIKLDKGSKSLKEKLAAVTPLVEELRIQKEERMKQFSDIKAQ 135

Query: 134 IQKICGEITG-------SLNISENPVVDESQLSVEKLDEYHCQLQELQKEKSERLHKVLE 193
           I+KI GEI+G       ++NIS    ++E  L++  L+EY   L+ LQKEKS+RL+KVL 
Sbjct: 136 IEKISGEISGYSDHLNKAMNIS--LTLEEQDLTLRNLNEYQTHLRTLQKEKSDRLNKVLG 195

Query: 194 FVSTVHDLCAVLGIDFFGTVTEVHPSLNDATGVQSKSISNDTLSKLAKMVLALKEDKKQR 253
           +V+ VH LC VLG+DF  TV+ VHPSL+     QS +IS+ TL  L  M+  LK ++K R
Sbjct: 196 YVNEVHALCGVLGVDFSQTVSAVHPSLHRTDQEQSTNISDSTLEGLEHMIQKLKTERKSR 255

Query: 254 LHKLQELATQLVDLWNLMDTPSEERSLFDHVTCNISASVDEVTVPGALALDLIEQAEVEV 313
             KL+++   L +LWNLMDTP E+R+ F  VT  + +S   +T PG L+ + IEQ   EV
Sbjct: 256 FQKLKDVVASLFELWNLMDTPQEDRTKFGKVTYVVRSSEANITEPGILSTETIEQVSTEV 315

Query: 314 ERLDQLKASRMKEIAFKKQSELEEIFAHAHIEIDPEAAREKIMTLIDSGNVEPSELLDDM 373
           + L +LKASRMKE+  K++SELE++    HI+ D   + EK   LIDSG V+PSELL ++
Sbjct: 316 DSLSKLKASRMKELVMKRRSELEDLCRLTHIQPDTSTSAEKSTALIDSGLVDPSELLANI 375

Query: 374 DNKIAKAKEEVLSRKEILDKVEKWMLACEEESWLEDYNRDDNRYNASRGAHLNLKRAEKA 433
           + +I K K+E  SRK+I+D++++W+ ACEEE+WLE+YN D+NRY+A RG H+NLKRAE+A
Sbjct: 376 EMQINKIKDEAQSRKDIMDRIDRWLSACEEENWLEEYNLDENRYSAGRGGHVNLKRAERA 435

Query: 434 RILVNKIPALVDTLIAKTRAWEEDRGISFIYDGVPLLAMLDEYTMLRHEREEEKRRFRDH 493
           R+ +NKIP +VDTLI KT  WEED   SF+YDGV L+ +L++Y + R             
Sbjct: 436 RVTINKIPGMVDTLIKKTLVWEEDMQKSFLYDGVRLVNILEDYKLTRXXXXXXXXXXXXX 495

Query: 494 KKFHEQQSTDQESITNSRPSPARPIGTKKAAG---PRANGAVNGTPSSRRLSL------- 553
                   T +ESI  S+PSP R    +K  G      NG+V  TP  RR S+       
Sbjct: 496 XXXXXXXXTQRESIYGSKPSPRRSSSFRKPNGFNISNGNGSVPPTP--RRGSVGTTTPDV 555

Query: 554 -----NQNGSRSMNKDGKRDGMRPMTPVKYDAISKEDSGSHVSGTEPVSSSP 582
                + +G    N   K       TP+ Y A+ KED+ S  + T   SS P
Sbjct: 556 LLTPRSYSGHHRQNGYFKEVRRLSTTPLNYVAMQKEDTVS-TTYTSIYSSEP 602

BLAST of Carg25350-RA vs. Swiss-Prot
Match: sp|Q9FLP0|MA651_ARATH (65-kDa microtubule-associated protein 1 OS=Arabidopsis thaliana OX=3702 GN=MAP65-1 PE=1 SV=1)

HSP 1 Score: 756.9 bits (1953), Expect = 1.6e-217
Identity = 418/589 (70.97%), Postives = 484/589 (82.17%), Query Frame = 0

Query: 1   MAVEDTQNPLQGETTCGTLLQKLQEIWDEVGESDEDRDKMLLQIDQECLDVYKRKVELAD 60
           MAV DT++P  GE TCGTLL+KLQEIWDEVGESD++RDK+LLQI+QECLDVYKRKVE A 
Sbjct: 1   MAVTDTESPHLGEITCGTLLEKLQEIWDEVGESDDERDKLLLQIEQECLDVYKRKVEQAA 60

Query: 61  KSRAKLLQSLSDAKHELSNLLSALGEKSFSEFPESASGTIKEQLAAIAPALEQLWKQKEK 120
           KSRA+LLQ+LSDA  ELS+L  +LG+KS    P+ +SGTIKEQLAAIAPALEQLW+QKE+
Sbjct: 61  KSRAELLQTLSDANAELSSLTMSLGDKSLVGIPDKSSGTIKEQLAAIAPALEQLWQQKEE 120

Query: 121 RVKEFFDVQSEIQKICGEITGSLNISENPVVDESQLSVEKLDEYHCQLQELQKEKSERLH 180
           RV+EF DVQS+IQKICG+I G L+ +E P+VDES LS++KLD++  QLQELQKEKS+RL 
Sbjct: 121 RVREFSDVQSQIQKICGDIAGGLS-NEVPIVDESDLSLKKLDDFQSQLQELQKEKSDRLR 180

Query: 181 KVLEFVSTVHDLCAVLGIDFFGTVTEVHPSLNDATGVQSKSISNDTLSKLAKMVLALKED 240
           KVLEFVSTVHDLCAVLG+DF  TVTEVHPSL++ T VQSKSISN+TLS+LAK VL LK+D
Sbjct: 181 KVLEFVSTVHDLCAVLGLDFLSTVTEVHPSLDEDTSVQSKSISNETLSRLAKTVLTLKDD 240

Query: 241 KKQRLHKLQELATQLVDLWNLMDTPSEERSLFDHVTCNISASVDEVTVPGALALDLIEQA 300
           K           TQL+DLWNLMDTP EER LFDHVTCNIS+SVDEVTVPGALA DLIEQA
Sbjct: 241 KXXXXXXXXXXXTQLIDLWNLMDTPDEERELFDHVTCNISSSVDEVTVPGALARDLIEQA 300

Query: 301 EVEVERLDQLKASRMKEIAFKKQSELEEIFAHAHIEIDPEAAREKIMTLIDSGNVEPSEL 360
           EVEV+RLDQLKASRMKEIAFKKQSELEEI+A AH+E++PE+ARE+IM+LIDSGNVEP+EL
Sbjct: 301 EVEVDRLDQLKASRMKEIAFKKQSELEEIYARAHVEVNPESARERIMSLIDSGNVEPTEL 360

Query: 361 LDDMDNKIAKAKEEVLSRKEILDKVEKWMLACEEESWLEDYNRDDNRYNASRGAHLNLKR 420
           L DMD++I+KAKEE  SRK+ILD+VEKWM ACEEESWLEDYNRD NRY+ASRGAHLNLKR
Sbjct: 361 LADMDSQISKAKEEAFSRKDILDRVEKWMSACEEESWLEDYNRDQNRYSASRGAHLNLKR 420

Query: 421 AEKARILVNKIPALVDTLIAKTRAWEEDRGISFIYDGVPLLAMLDEYTMLRHEREEEKRR 480
           AEKARILV+KIPA+VDTL+AKTRAWEE+  +SF YDGVPLLAMLDEY MLR EREEE   
Sbjct: 421 AEKARILVSKIPAMVDTLVAKTRAWEEEHSMSFAYDGVPLLAMLDEYGMLRQEREEEXXX 480

Query: 481 FRDHKKFHEQQSTDQESITNSRPSPARPIGTKKAAGPRA-NGAVNGTPSSRRLSL--NQN 540
                          ES  ++RPSPARP+  KK  GPRA NG  NGT  +RRLSL  NQN
Sbjct: 481 XXXXXXXXXXXXXXXESAFSTRPSPARPVSAKKTVGPRANNGGANGT-HNRRLSLNANQN 540

Query: 541 GSRSMNKD-GKRDGM-RPMTPVKYDAISKEDSGSH-VSGT--EPVSSSP 582
           GSRS  K+ G+R+ + RP  P  Y AISKE++ S  VSG     V +SP
Sbjct: 541 GSRSTAKEAGRRETLNRPAAPTNYVAISKEEAASSPVSGAADHQVPASP 587

BLAST of Carg25350-RA vs. Swiss-Prot
Match: sp|Q8LEG3|MA652_ARATH (65-kDa microtubule-associated protein 2 OS=Arabidopsis thaliana OX=3702 GN=MAP65-2 PE=1 SV=1)

HSP 1 Score: 678.7 bits (1750), Expect = 5.7e-194
Identity = 385/585 (65.81%), Postives = 448/585 (76.58%), Query Frame = 0

Query: 1   MAVEDTQNPLQGETTCGTLLQKLQEIWDEVGESDEDRDKMLLQIDQECLDVYKRKVELAD 60
           MAV + +NPL GE TCGTLLQKLQEIWDEVGESDE+RDK+LLQI++ECL+VYK+KVELA 
Sbjct: 1   MAVTEAENPLLGEITCGTLLQKLQEIWDEVGESDEERDKLLLQIEEECLNVYKKKVELAA 60

Query: 61  KSRAKLLQSLSDAKHELSNLLSALGEKSFSEFPESASGTIKEQLAAIAPALEQLWKQKEK 120
           KSRA+LLQ+LSDA  ELSNL +ALGEKS+ + P+  SGTIKEQL+AIAPALEQLW+QKE+
Sbjct: 61  KSRAELLQTLSDATVELSNLTTALGEKSYIDIPDKTSGTIKEQLSAIAPALEQLWQQKEE 120

Query: 121 RVKEFFDVQSEIQKICGEITGSLNISENPVVDESQLSVEKLDEYHCQLQELQKEKSERLH 180
           RV+ F DVQS+IQKIC EI G LN   + VVDE+ LS+++LD++  +LQELQKEKS+RL 
Sbjct: 121 RVRAFSDVQSQIQKICEEIAGGLNNGPH-VVDETDLSLKRLDDFQRKLQELQKEKSDRLQ 180

Query: 181 KVLEFVSTVHDLCAVLGIDFFGTVTEVHPSLNDATGVQSKSISNDTLSKLAKMVLALKED 240
           KVLEFVSTVHDLCAVL +DF  TVTEVHPSL++A GVQ+KSISN+TL++L          
Sbjct: 181 KVLEFVSTVHDLCAVLRLDFLSTVTEVHPSLDEANGVQTKSISNETLARLXXXXXXXXXX 240

Query: 241 KKQRLHKLQELATQLVDLWNLMDTPSEERSLFDHVTCNISASVDEVTVPGALALDLIEQA 300
                                     EER LFDHVT NISASV EVT  GALALDLIEQA
Sbjct: 241 XXXXXXXXXXXXXXXXXXXXXXXXXXEERELFDHVTSNISASVHEVTASGALALDLIEQA 300

Query: 301 EVEVERLDQLKASRMKEIAFKKQSELEEIFAHAHIEIDPEAAREKIMTLIDSGNVEPSEL 360
           EVEV+RLDQLK+SRMKEIAFKKQSELEEI+A AHIEI PE  RE+IM+LID+GN EP+EL
Sbjct: 301 EVEVDRLDQLKSSRMKEIAFKKQSELEEIYARAHIEIKPEVVRERIMSLIDAGNTEPTEL 360

Query: 361 LDDMDNKIAKAKEEVLSRKEILDKVEKWMLACEEESWLEDYNRDDNRYNASRGAHLNLKR 420
           L DMD++IAKAKEE  SRKEILD+VEKWM ACEEESWLEDYNRD NRY+ASRGAHLNLKR
Sbjct: 361 LADMDSQIAKAKEEAFSRKEILDRVEKWMSACEEESWLEDYNRDQNRYSASRGAHLNLKR 420

Query: 421 AEKARILVNKIPALVDTLIAKTRAWEEDRGISFIYDGVPLLAMLDEYTMLRHEREEEKRR 480
           AEKARILV+KI A+VDTLIAKTRAWEE+  +SF YDGVPLLAMLDEYTMLR ERE     
Sbjct: 421 AEKARILVSKITAMVDTLIAKTRAWEEENSMSFEYDGVPLLAMLDEYTMLRQEREXXXXX 480

Query: 481 FRDHKKFHEQQSTDQESITNSRPSPARPIGTKKAAGPRAN-GAVNGTPSSRRLSL--NQN 540
                       TDQES   S+PSPARP+  KK  G R N G +N TP  RRLS+  NQN
Sbjct: 481 XXXXXXXXXXPHTDQESAFGSKPSPARPVSAKKPVGTRVNGGGLNETP-MRRLSMNSNQN 540

Query: 541 GSRSMNKDGKRDGMRPM-TPVKYDAISKEDSGSHVSGTEPVSSSP 582
           GS+S     KRD +  + +P    A +K+D+ S VS  +PV +SP
Sbjct: 541 GSKS-----KRDSLNKIASPSNIVANTKDDAASPVSRADPVMASP 578

BLAST of Carg25350-RA vs. Swiss-Prot
Match: sp|Q9FHM4|MA653_ARATH (65-kDa microtubule-associated protein 3 OS=Arabidopsis thaliana OX=3702 GN=MAP65-3 PE=1 SV=1)

HSP 1 Score: 469.2 bits (1206), Expect = 6.8e-131
Identity = 247/502 (49.20%), Postives = 363/502 (72.31%), Query Frame = 0

Query: 10  LQGETTCGTLLQKLQEIWDEVGESDEDRDKMLLQIDQECLDVYKRKVELADKSRAKLLQS 69
           LQ ETTCG+LL +LQ IWDEVGE++ DRD+MLL++++ECL+VY+RKV+ A++ RA+L Q+
Sbjct: 10  LQVETTCGSLLFELQIIWDEVGETETDRDQMLLELERECLEVYRRKVDQANRCRAQLRQA 69

Query: 70  LSDAKHELSNLLSALGEKSFS-EFPESASGTIKEQLAAIAPALEQLWKQKEKRVKEFFDV 129
           ++DA+ +L+ + SA+GE+       + + G++K++L  I P LE++ K+K +R  +F  V
Sbjct: 70  IADAEAQLAAICSAMGERPVHIRQSDQSVGSLKQELGRILPELEEMQKRKVERRNQFIVV 129

Query: 130 QSEIQKICGEITGSLN-ISENPVVDESQLSVEKLDEYHCQLQELQKEKSERLHKVLEFVS 189
             +I  I  +I G    +   P++DE+ LS+ KL+E HCQLQ LQKEK +R+  + + + 
Sbjct: 130 MEQIDSITNDIKGQGELVHSEPLIDETNLSMRKLEELHCQLQVLQKEKIDRVETIRKHLC 189

Query: 190 TVHDLCAVLGIDFFGTVTEVHPSLNDATGVQSKSISNDTLSKLAKMVLALKEDKKQRLHK 249
           T++  C+VLG+DF   V +V+P+L+D  G   +S+S+ T+ KL   V  L E K QR+ +
Sbjct: 190 TLYSHCSVLGMDFNEVVGQVNPTLSDPEG--PRSLSDHTIEKLGAAVQKLMEVKIQRMQR 249

Query: 250 LQELATQLVDLWNLMDTPSEERSLFDHVTCNISASVDEVTVPGALALDLIEQAEVEVERL 309
           LQ+LAT +++LWNLMDTP EE+  + H+TCNI+AS  E+T   +L+ D I+  E EV RL
Sbjct: 250 LQDLATTMLELWNLMDTPIEEQQEYQHITCNIAASEHEITEANSLSEDFIKYVEAEVVRL 309

Query: 310 DQLKASRMKEIAFKKQSELEEIFAHAHIEIDPEAAREKIMTLIDSGNVEPSELLDDMDNK 369
           D++KAS+MKE+  KK+SELEEI    H+    ++A ++ +  I+SG V+ + +L+ ++  
Sbjct: 310 DEVKASKMKELVLKKRSELEEICRKTHLLPVSDSAIDQTIVAIESGIVDATMVLEHLEQH 369

Query: 370 IAKAKEEVLSRKEILDKVEKWMLACEEESWLEDYNRDDNRYNASRGAHLNLKRAEKARIL 429
           I+K KEE LSRKEIL++VEKW+ AC+EESWLE+YNRDDNRYNA RGAHL LKRAEKAR L
Sbjct: 370 ISKIKEEALSRKEILERVEKWLSACDEESWLEEYNRDDNRYNAGRGAHLTLKRAEKARNL 429

Query: 430 VNKIPALVDTLIAKTRAWEEDRGISFIYDGVPLLAMLDEYTMLRHEREEEKRRFRDHKKF 489
           V K+P +V+ L +KT  WE++ GI F+YDG+ LL+ML+EY +LR EREEE RR RD KK 
Sbjct: 430 VTKLPGMVEALASKTIVWEQENGIEFLYDGIRLLSMLEEYNILRQEREEEHRRQRDQKKL 489

Query: 490 HEQQSTDQESITNSRPSPARPI 510
             Q   +QE++  S+PSP++P+
Sbjct: 490 QGQLIAEQEALYGSKPSPSKPL 509

BLAST of Carg25350-RA vs. Swiss-Prot
Match: sp|Q9ZVJ3|MA655_ARATH (65-kDa microtubule-associated protein 5 OS=Arabidopsis thaliana OX=3702 GN=MAP65-5 PE=1 SV=2)

HSP 1 Score: 451.4 bits (1160), Expect = 1.5e-125
Identity = 254/555 (45.77%), Postives = 375/555 (67.57%), Query Frame = 0

Query: 14  TTCGTLLQKLQEIWDEVGESDEDRDKMLLQIDQECLDVYKRKVELADKSRAKLLQSLSDA 73
           TTC +LL++LQ IWDE+GES  +RDKMLL+++QECLD+Y +KVE   K RA+L +SL+ A
Sbjct: 7   TTCTSLLEELQMIWDEIGESYNERDKMLLELEQECLDIYNKKVEKTRKFRAELQRSLAQA 66

Query: 74  KHELSNLLSALGEK-SFSEFPESASGTIKEQLAAIAPALEQLWKQKEKRVKEFFDVQSEI 133
           + E+++L+SALGEK SF++      G++KEQ++++ P LE L  +K++R KE  +  ++I
Sbjct: 67  EAEIASLMSALGEKVSFAK----KEGSLKEQISSVKPVLEDLLMKKDRRRKELSETLNQI 126

Query: 134 QKICGEITGS-LNISENPVVDESQLSVEKLDEYHCQLQELQKEKSERLHKVLEFVSTVHD 193
            +I   I G+   +S    VDES L+  KLDE    LQ+L+ EK+ RL KV  ++S VH+
Sbjct: 127 AEITSNIAGNDYTVSSGSEVDESDLTQRKLDELRADLQDLRNEKAVRLQKVNSYISAVHE 186

Query: 194 LCAVLGIDFFGTVTEVHPSLNDATGVQSKSISNDTLSKLAKMVLALKEDKKQRLHKLQEL 253
           L  +L  DF   +  VH SL + +   SKSISNDTL++  ++V +LK +K +RL KLQ L
Sbjct: 187 LSEILSFDFSKALNSVHSSLTEFSKTHSKSISNDTLARFTELVKSLKAEKHERLLKLQGL 246

Query: 254 ATQLVDLWNLMDTPSEERSLFDHVTCNISASVDEVTVPGALALDLIEQAEVEVERLDQLK 313
              + +LWNLM+TP +ER  FDH +  +S+  D+    G L+LD+I +AE EV RL+ LK
Sbjct: 247 GRSMQELWNLMETPMDERRRFDHCSSLLSSLPDDALKKGCLSLDIIREAEDEVRRLNSLK 306

Query: 314 ASRMKEIAFKKQSELEEIFAHAHIEIDPEAAREKIMTLIDSGNVEPSELLDDMDNKIAKA 373
           +S+MKE+ FK+Q ELEEI    H++I+ +AAR+ ++ LI+SG+ + S++L  +D +I KA
Sbjct: 307 SSKMKELVFKRQCELEEICRGNHMDINSDAARKSLVELIESGDGDLSDILASIDGQIEKA 366

Query: 374 KEEVLSRKEILDKVEKWMLACEEESWLEDYNRDDNRYNASRGAHLNLKRAEKARILVNKI 433
           +EE LSRKEILDKV+KW  A EEE+WL+DY +D+NR++A RGAH NLKRAEKAR L++KI
Sbjct: 367 REEALSRKEILDKVDKWRHAKEEETWLDDYEKDENRFSAVRGAHKNLKRAEKARSLISKI 426

Query: 434 PALVDTLIAKTRAWEEDRGISFIYDGVPLLAMLDEYTMLRHEREEEKRRFRDHKKFHEQQ 493
           PA+VD L  K +AWE++RG+ F+ D  PLL  L++  ++R +REEEKR+FR+ K+   Q 
Sbjct: 427 PAMVDVLTTKVKAWEKERGVPFLCDKQPLLQTLEDDIVIRAQREEEKRQFREQKRLQGQL 486

Query: 494 STDQESITNSRPSPARPIGTKKAAGPRANGAVNGTPSSRRLSLNQNGSRSMNKDGKRDGM 553
           +T++E+   S+ +  +P+G            V  TP  RR  +     RS+   GK    
Sbjct: 487 ATEKEAKYGSKSAKKKPLGQS-----LNTDNVTKTPIGRR--IGNTPGRSVTSGGKDYRG 546

Query: 554 RPMTPVKYDAISKED 567
             + P+ Y A+ K+D
Sbjct: 547 NAVIPLNYVALQKDD 550

BLAST of Carg25350-RA vs. Swiss-Prot
Match: sp|Q9SIS3|MA656_ARATH (65-kDa microtubule-associated protein 6 OS=Arabidopsis thaliana OX=3702 GN=MAP65-6 PE=1 SV=1)

HSP 1 Score: 433.0 bits (1112), Expect = 5.4e-120
Identity = 257/592 (43.41%), Postives = 380/592 (64.19%), Query Frame = 0

Query: 14  TTCGTLLQKLQEIWDEVGESDEDRDKMLLQIDQECLDVYKRKVELADKSRAKLLQSLSDA 73
           TTC  LL++LQ+IW E+GE++ ++D+ML+++++ECL +Y+RKV+ A  S+AKL QS++  
Sbjct: 16  TTCNNLLRELQKIWVEIGETETEKDRMLMELERECLQIYQRKVDEAANSKAKLHQSVASI 75

Query: 74  KHELSNLLSALGEKSFSE--FPESASGTIKEQLAAIAPALEQLWKQKEKRVKEFFDVQSE 133
           + E+++L++ALG  + +     +  S ++KE+LAA+ P +E+L  QKE+R+K+F D++++
Sbjct: 76  EAEVASLMAALGVLNINSPIKLDKGSKSLKEKLAAVTPLVEELRIQKEERMKQFSDIKAQ 135

Query: 134 IQKICGEITG-------SLNISENPVVDESQLSVEKLDEYHCQLQELQKEKSERLHKVLE 193
           I+KI GEI+G       ++NIS    ++E  L++  L+EY   L+ LQKEKS+RL+KVL 
Sbjct: 136 IEKISGEISGYSDHLNKAMNIS--LTLEEQDLTLRNLNEYQTHLRTLQKEKSDRLNKVLG 195

Query: 194 FVSTVHDLCAVLGIDFFGTVTEVHPSLNDATGVQSKSISNDTLSKLAKMVLALKEDKKQR 253
           +V+ VH LC VLG+DF  TV+ VHPSL+     QS +IS+ TL  L  M+  LK ++K R
Sbjct: 196 YVNEVHALCGVLGVDFSQTVSAVHPSLHRTDQEQSTNISDSTLEGLEHMIQKLKTERKSR 255

Query: 254 LHKLQELATQLVDLWNLMDTPSEERSLFDHVTCNISASVDEVTVPGALALDLIEQAEVEV 313
             KL+++   L +LWNLMDTP E+R+ F  VT  + +S   +T PG L+ + IEQ   EV
Sbjct: 256 FQKLKDVVASLFELWNLMDTPQEDRTKFGKVTYVVRSSEANITEPGILSTETIEQVSTEV 315

Query: 314 ERLDQLKASRMKEIAFKKQSELEEIFAHAHIEIDPEAAREKIMTLIDSGNVEPSELLDDM 373
           + L +LKASRMKE+  K++SELE++    HI+ D   + EK   LIDSG V+PSELL ++
Sbjct: 316 DSLSKLKASRMKELVMKRRSELEDLCRLTHIQPDTSTSAEKSTALIDSGLVDPSELLANI 375

Query: 374 DNKIAKAKEEVLSRKEILDKVEKWMLACEEESWLEDYNRDDNRYNASRGAHLNLKRAEKA 433
           + +I K K+E  SRK+I+D++++W+ ACEEE+WLE+YN D+NRY+A RG H+NLKRAE+A
Sbjct: 376 EMQINKIKDEAQSRKDIMDRIDRWLSACEEENWLEEYNLDENRYSAGRGGHVNLKRAERA 435

Query: 434 RILVNKIPALVDTLIAKTRAWEEDRGISFIYDGVPLLAMLDEYTMLRHEREEEKRRFRDH 493
           R+ +NKIP +VDTLI KT  WEED   SF+YDGV L+ +L++Y + R             
Sbjct: 436 RVTINKIPGMVDTLIKKTLVWEEDMQKSFLYDGVRLVNILEDYKLTRXXXXXXXXXXXXX 495

Query: 494 KKFHEQQSTDQESITNSRPSPARPIGTKKAAG---PRANGAVNGTPSSRRLSL------- 553
                   T +ESI  S+PSP R    +K  G      NG+V  TP  RR S+       
Sbjct: 496 XXXXXXXXTQRESIYGSKPSPRRSSSFRKPNGFNISNGNGSVPPTP--RRGSVGTTTPDV 555

Query: 554 -----NQNGSRSMNKDGKRDGMRPMTPVKYDAISKEDSGSHVSGTEPVSSSP 582
                + +G    N   K       TP+ Y A+ KED+ S  + T   SS P
Sbjct: 556 LLTPRSYSGHHRQNGYFKEVRRLSTTPLNYVAMQKEDTVS-TTYTSIYSSEP 602

BLAST of Carg25350-RA vs. TrEMBL
Match: tr|A0A0A0L2B7|A0A0A0L2B7_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G073820 PE=4 SV=1)

HSP 1 Score: 1045.8 bits (2703), Expect = 3.6e-302
Identity = 546/581 (93.98%), Postives = 563/581 (96.90%), Query Frame = 0

Query: 1   MAVEDTQNPLQGETTCGTLLQKLQEIWDEVGESDEDRDKMLLQIDQECLDVYKRKVELAD 60
           MAV DTQ PLQGETTCGTLLQKLQEIWDEVGE+DE+RDKMLLQIDQECLDVYKRKVELAD
Sbjct: 1   MAVADTQIPLQGETTCGTLLQKLQEIWDEVGETDEERDKMLLQIDQECLDVYKRKVELAD 60

Query: 61  KSRAKLLQSLSDAKHELSNLLSALGEKSFSEFPESASGTIKEQLAAIAPALEQLWKQKEK 120
           KSRAKLLQSLSDAKHELS+LLSALGEKSFSEFPE ASGTIKEQLAAIAPALE+LWKQKE+
Sbjct: 61  KSRAKLLQSLSDAKHELSSLLSALGEKSFSEFPEKASGTIKEQLAAIAPALEKLWKQKEQ 120

Query: 121 RVKEFFDVQSEIQKICGEITGSLNISENPVVDESQLSVEKLDEYHCQLQELQKEKSERLH 180
           RVKEFFDVQSEIQKICGEI GSLN+SENPVVDESQLSVEKLDEYHCQLQELQKEKS+RLH
Sbjct: 121 RVKEFFDVQSEIQKICGEIAGSLNLSENPVVDESQLSVEKLDEYHCQLQELQKEKSDRLH 180

Query: 181 KVLEFVSTVHDLCAVLGIDFFGTVTEVHPSLNDATGVQSKSISNDTLSKLAKMVLALKED 240
           KVLEFVSTVHDLCAVLGIDFF TVTEVHPSLNDATGVQSKSISNDTLSKLAKMVLALKED
Sbjct: 181 KVLEFVSTVHDLCAVLGIDFFATVTEVHPSLNDATGVQSKSISNDTLSKLAKMVLALKED 240

Query: 241 KKQRLHKLQELATQLVDLWNLMDTPSEERSLFDHVTCNISASVDEVTVPGALALDLIEQA 300
           KKQRLHKLQELATQL+DLWNLMDTPSEERSLFDHVTCNISASVDEVTVPGALALDLIEQA
Sbjct: 241 KKQRLHKLQELATQLIDLWNLMDTPSEERSLFDHVTCNISASVDEVTVPGALALDLIEQA 300

Query: 301 EVEVERLDQLKASRMKEIAFKKQSELEEIFAHAHIEIDPEAAREKIMTLIDSGNVEPSEL 360
           EVEVERLDQLKASRMKEIAFKKQSELEEIF+HAHIEIDPEAAREKIM LIDSGNVEP+EL
Sbjct: 301 EVEVERLDQLKASRMKEIAFKKQSELEEIFSHAHIEIDPEAAREKIMALIDSGNVEPAEL 360

Query: 361 LDDMDNKIAKAKEEVLSRKEILDKVEKWMLACEEESWLEDYNRDDNRYNASRGAHLNLKR 420
           LDDMD++I KAKEEVLSRKEILDKVEKWMLACEEESWLEDYNRDDNRYNASRGAHLNLKR
Sbjct: 361 LDDMDDQITKAKEEVLSRKEILDKVEKWMLACEEESWLEDYNRDDNRYNASRGAHLNLKR 420

Query: 421 AEKARILVNKIPALVDTLIAKTRAWEEDRGISFIYDGVPLLAMLDEYTMLRHEREEEKRR 480
           AEKARILVNKIPALVDTLIAKTRAWEEDRGISFIYDGVPLLAMLDEYTMLRHEREEEKRR
Sbjct: 421 AEKARILVNKIPALVDTLIAKTRAWEEDRGISFIYDGVPLLAMLDEYTMLRHEREEEKRR 480

Query: 481 FRDHKKFHEQQSTDQESITNSRPSPARPIGTKKAAGPRANGAVNGTPSSRRLSLNQNGSR 540
            RDHKKFH+QQ+TD E ITNSRPSP R I TKKA G RANGA NGTPSSRRLSLNQNGSR
Sbjct: 481 LRDHKKFHDQQTTDPEIITNSRPSPVRTIATKKAGGARANGAANGTPSSRRLSLNQNGSR 540

Query: 541 SMNKDGKRDGMRPMTPVKYDAISKEDSGSHVSGTEPVSSSP 582
           SMNKD KRD MRPMTPVKYDAISK+DSGSH+SGTEPVS+SP
Sbjct: 541 SMNKDSKRDSMRPMTPVKYDAISKDDSGSHISGTEPVSASP 581

BLAST of Carg25350-RA vs. TrEMBL
Match: tr|A0A1S3BM91|A0A1S3BM91_CUCME (65-kDa microtubule-associated protein 1-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103491082 PE=4 SV=1)

HSP 1 Score: 1023.1 bits (2644), Expect = 2.5e-295
Identity = 538/581 (92.60%), Postives = 555/581 (95.52%), Query Frame = 0

Query: 1   MAVEDTQNPLQGETTCGTLLQKLQEIWDEVGESDEDRDKMLLQIDQECLDVYKRKVELAD 60
           MAV DTQ PLQGETTCGTLLQKLQEIWDEVGE+DE+RDKMLLQIDQECLDVYKRKVELAD
Sbjct: 1   MAVADTQIPLQGETTCGTLLQKLQEIWDEVGETDEERDKMLLQIDQECLDVYKRKVELAD 60

Query: 61  KSRAKLLQSLSDAKHELSNLLSALGEKSFSEFPESASGTIKEQLAAIAPALEQLWKQKEK 120
           KSRAKLLQSLSDAKHELS+LLSALGEKSFSEFPE ASGTIKEQLAAIAPALE+LWKQKE+
Sbjct: 61  KSRAKLLQSLSDAKHELSSLLSALGEKSFSEFPEKASGTIKEQLAAIAPALEKLWKQKEQ 120

Query: 121 RVKEFFDVQSEIQKICGEITGSLNISENPVVDESQLSVEKLDEYHCQLQELQKEKSERLH 180
           RVKEFFDVQSEIQKICGEI GSLN+SENPVVDESQLS+EKLDEYHCQLQELQKEKSERLH
Sbjct: 121 RVKEFFDVQSEIQKICGEIAGSLNLSENPVVDESQLSIEKLDEYHCQLQELQKEKSERLH 180

Query: 181 KVLEFVSTVHDLCAVLGIDFFGTVTEVHPSLNDATGVQSKSISNDTLSKLAKMVLALKED 240
           KVLEFVSTVHDLCAVLGIDFF TVTEVHPSLNDATGVQSKSISNDTLSKLAKMVLALKED
Sbjct: 181 KVLEFVSTVHDLCAVLGIDFFATVTEVHPSLNDATGVQSKSISNDTLSKLAKMVLALKED 240

Query: 241 KKQRLHKLQELATQLVDLWNLMDTPSEERSLFDHVTCNISASVDEVTVPGALALDLIEQA 300
           KKQRLHKLQELATQL+DLWNLMDTPSEERSLFDHVTCNISASVDEVTVPGALALDLIEQA
Sbjct: 241 KKQRLHKLQELATQLIDLWNLMDTPSEERSLFDHVTCNISASVDEVTVPGALALDLIEQA 300

Query: 301 EVEVERLDQLKASRMKEIAFKKQSELEEIFAHAHIEIDPEAAREKIMTLIDSGNVEPSEL 360
           EVEVERLDQLKASRMKEIAFKKQSELEEIF+HAHIEIDPEAAREKIM LIDSGNVEP+EL
Sbjct: 301 EVEVERLDQLKASRMKEIAFKKQSELEEIFSHAHIEIDPEAAREKIMALIDSGNVEPAEL 360

Query: 361 LDDMDNKIAKAKEEVLSRKEILDKVEKWMLACEEESWLEDYNRDDNRYNASRGAHLNLKR 420
           LDDMD++IAKAKEEVLSRKEILDKVEKWMLACEEESWLEDYNRDDNRYNASRGAHLNLKR
Sbjct: 361 LDDMDDQIAKAKEEVLSRKEILDKVEKWMLACEEESWLEDYNRDDNRYNASRGAHLNLKR 420

Query: 421 AEKARILVNKIPALVDTLIAKTRAWEEDRGISFIYDGVPLLAMLDEYTMLRHEREEEKRR 480
           AEKARILVNKIPALVDTLIAKTR WEEDRGISFIYDGVPLLAMLDEYTMLRHEREE    
Sbjct: 421 AEKARILVNKIPALVDTLIAKTRTWEEDRGISFIYDGVPLLAMLDEYTMLRHEREEXXXX 480

Query: 481 FRDHKKFHEQQSTDQESITNSRPSPARPIGTKKAAGPRANGAVNGTPSSRRLSLNQNGSR 540
               KKFH+QQ+TD E ITNSRPSP R   TKKA GPR NGA NGTPSSRRLSLNQNGSR
Sbjct: 481 XXVKKKFHDQQTTDPEIITNSRPSPVRTT-TKKAGGPRVNGAANGTPSSRRLSLNQNGSR 540

Query: 541 SMNKDGKRDGMRPMTPVKYDAISKEDSGSHVSGTEPVSSSP 582
           SMNKD KRD MRPMTPVKYDAISK+DSGSH+SGTEPVS+SP
Sbjct: 541 SMNKDSKRDSMRPMTPVKYDAISKDDSGSHISGTEPVSASP 580

BLAST of Carg25350-RA vs. TrEMBL
Match: tr|A0A2P4H132|A0A2P4H132_QUESU (65-kDa microtubule-associated protein 1 OS=Quercus suber OX=58331 GN=CFP56_48531 PE=4 SV=1)

HSP 1 Score: 921.8 bits (2381), Expect = 7.8e-265
Identity = 488/588 (82.99%), Postives = 532/588 (90.48%), Query Frame = 0

Query: 1   MAVEDTQNPLQGETTCGTLLQKLQEIWDEVGESDEDRDKMLLQIDQECLDVYKRKVELAD 60
           MAV D QNPL GETTCG+LLQKLQEIWDEVGESDE+RDKMLLQ++Q+CLDVYKRKVE A 
Sbjct: 1   MAVTDVQNPLLGETTCGSLLQKLQEIWDEVGESDEERDKMLLQLEQDCLDVYKRKVEQAA 60

Query: 61  KSRAKLLQSLSDAKHELSNLLSALGEKSFSEFPESASGTIKEQLAAIAPALEQLWKQKEK 120
           KSRA+LLQ+LSDAK ELS+LLSALGEKSF+  P+  SGTIKEQLAAIAPALEQLWKQKE+
Sbjct: 61  KSRAQLLQALSDAKLELSSLLSALGEKSFAGIPDKTSGTIKEQLAAIAPALEQLWKQKEE 120

Query: 121 RVKEFFDVQSEIQKICGEITGSLNISE---NPVVDESQLSVEKLDEYHCQLQELQKEKSE 180
           R+KEF DVQS+IQKICGEI G+LN+S+    P VDE+ LS+++LDEYH QLQELQKEKSE
Sbjct: 121 RIKEFSDVQSQIQKICGEIAGTLNLSDQAGTPAVDEADLSLKRLDEYHGQLQELQKEKSE 180

Query: 181 RLHKVLEFVSTVHDLCAVLGIDFFGTVTEVHPSLNDATGVQSKSISNDTLSKLAKMVLAL 240
           RLHKVLEFVSTVHDLCAVLG+DFF TVTEVHPSLND T VQSKSISNDTLS+LAK VLAL
Sbjct: 181 RLHKVLEFVSTVHDLCAVLGMDFFSTVTEVHPSLNDTTSVQSKSISNDTLSRLAKTVLAL 240

Query: 241 KEDKKQRLHKLQELATQLVDLWNLMDTPSEERSLFDHVTCNISASVDEVTVPGALALDLI 300
           KEDKKQRLHKLQELATQL+DLWNLMDTP EERSLFDHVTCNISASVDEVTVPGALALDLI
Sbjct: 241 KEDKKQRLHKLQELATQLIDLWNLMDTPVEERSLFDHVTCNISASVDEVTVPGALALDLI 300

Query: 301 EQAEVEVERLDQLKASRMKEIAFKKQSELEEIFAHAHIEIDPEAAREKIMTLIDSGNVEP 360
           EQAEVEVERLDQLKASRMKEIAFKKQ+ELE+IFAHAHIEIDPEAAREKIM LIDSG+VEP
Sbjct: 301 EQAEVEVERLDQLKASRMKEIAFKKQTELEDIFAHAHIEIDPEAAREKIMALIDSGDVEP 360

Query: 361 SELLDDMDNKIAKAKEEVLSRKEILDKVEKWMLACEEESWLEDYNRDDNRYNASRGAHLN 420
           +ELL DMDN+IA AK+E LSRK+ILDKVEKWM ACEEESWLEDYNRD+NRYNASRGAHLN
Sbjct: 361 AELLADMDNQIANAKDEALSRKDILDKVEKWMSACEEESWLEDYNRDENRYNASRGAHLN 420

Query: 421 LKRAEKARILVNKIPALVDTLIAKTRAWEEDRGISFIYDGVPLLAMLDEYTMLRHEREEE 480
           LKRAEKARILVNKIPALV+TL+ KTRAWEE+RGI F YDGVPLLAML+EY MLRHEREEE
Sbjct: 421 LKRAEKARILVNKIPALVETLVTKTRAWEEERGIPFTYDGVPLLAMLEEYAMLRHEREEE 480

Query: 481 KRRFRDHKKFHEQQSTDQESITNSRPSPARPIGTKKAAGPRAN--GAVNGTPSSRRLSLN 540
           KR+ RD KKFHEQQ+T+QE+I  SRPSPARP GTKK  GPRAN  G  NGTP SRRLSLN
Sbjct: 481 KRKMRDQKKFHEQQNTEQEAIFGSRPSPARPAGTKKVVGPRANGGGGANGTP-SRRLSLN 540

Query: 541 --QNGSRSMNKDGKRDGMRPMTPVKYDAISKEDSGSHVSGTEPVSSSP 582
             QNGSRS  KDGKRD  RP+ PV Y AISKED+ SHVSG++PV +SP
Sbjct: 541 AHQNGSRSTTKDGKRDNTRPVAPVNYVAISKEDAASHVSGSDPVPASP 587

BLAST of Carg25350-RA vs. TrEMBL
Match: tr|W9R8X5|W9R8X5_9ROSA (Uncharacterized protein OS=Morus notabilis OX=981085 GN=L484_012448 PE=4 SV=1)

HSP 1 Score: 907.1 bits (2343), Expect = 2.0e-260
Identity = 481/584 (82.36%), Postives = 524/584 (89.73%), Query Frame = 0

Query: 1   MAVEDTQNPLQGETTCGTLLQKLQEIWDEVGESDEDRDKMLLQIDQECLDVYKRKVELAD 60
           MAV D+QNPL GETTCG+LLQKLQEIWDEVGESD++RDKMLLQ++QECLDVYKRKVE A 
Sbjct: 98  MAVTDSQNPLLGETTCGSLLQKLQEIWDEVGESDDERDKMLLQLEQECLDVYKRKVEQAA 157

Query: 61  KSRAKLLQSLSDAKHELSNLLSALGEKSFSEFPESASGTIKEQLAAIAPALEQLWKQKEK 120
           KSRA+LLQ+LSD K ELS LLSALGEKS    PE  SGTIKEQLAAIAPALEQLWKQKE+
Sbjct: 158 KSRAQLLQALSDGKLELSTLLSALGEKSSVGIPEKTSGTIKEQLAAIAPALEQLWKQKEE 217

Query: 121 RVKEFFDVQSEIQKICGEITGSLNISE---NPVVDESQLSVEKLDEYHCQLQELQKEKSE 180
           RVKE  DVQS+IQKICGEI G+ N+SE    PV DES L+++KLDEYH QLQELQKEKS+
Sbjct: 218 RVKEIADVQSQIQKICGEIAGNSNLSEQAGTPVFDESDLTLKKLDEYHAQLQELQKEKSD 277

Query: 181 RLHKVLEFVSTVHDLCAVLGIDFFGTVTEVHPSLNDATGVQSKSISNDTLSKLAKMVLAL 240
           RLHKVLEFVSTVHDLCAVLGIDFF TVTEVHPSLND+TG+QSKSISNDTLS+LAK VLAL
Sbjct: 278 RLHKVLEFVSTVHDLCAVLGIDFFSTVTEVHPSLNDSTGMQSKSISNDTLSRLAKTVLAL 337

Query: 241 KEDKKQRLHKLQELATQLVDLWNLMDTPSEERSLFDHVTCNISASVDEVTVPGALALDLI 300
           KEDKKQRLHKLQELA+QL+DLWNLMDTP EERSLFDHVTCNISASVDEVTVPGALALDLI
Sbjct: 338 KEDKKQRLHKLQELASQLIDLWNLMDTPDEERSLFDHVTCNISASVDEVTVPGALALDLI 397

Query: 301 EQAEVEVERLDQLKASRMKEIAFKKQSELEEIFAHAHIEIDPEAAREKIMTLIDSGNVEP 360
           EQAEVEVERLDQLK+SRMKEIAFK+Q+ELEEIFA AHIEIDPEAAR+KIM +IDSGNVEP
Sbjct: 398 EQAEVEVERLDQLKSSRMKEIAFKRQAELEEIFARAHIEIDPEAARDKIMAMIDSGNVEP 457

Query: 361 SELLDDMDNKIAKAKEEVLSRKEILDKVEKWMLACEEESWLEDYNRDDNRYNASRGAHLN 420
           +ELL  MDN+IAKAKEE LSRKEILDKVEKWM ACEEESWLEDYNRDDNRYNASRGAHLN
Sbjct: 458 AELLAGMDNEIAKAKEEALSRKEILDKVEKWMSACEEESWLEDYNRDDNRYNASRGAHLN 517

Query: 421 LKRAEKARILVNKIPALVDTLIAKTRAWEEDRGISFIYDGVPLLAMLDEYTMLRHEREEE 480
           LKRAEKARILVNKIPALVDTL+AKTRAWEEDRGISF YDGVPLLAMLDEY MLR EREEE
Sbjct: 518 LKRAEKARILVNKIPALVDTLVAKTRAWEEDRGISFTYDGVPLLAMLDEYAMLRQEREEE 577

Query: 481 KRRFRDHKKFHEQQSTDQESITNSRPSPARPIGTKKAAGPRANGAVNGTPSSRRLSLNQN 540
           KRR +D KK+ +QQ T+QE+I  S+PSPARP+GTKK  GPR NG  NGTP +RRLSLNQN
Sbjct: 578 KRRMKDQKKYTDQQHTEQEAIFGSKPSPARPVGTKKVVGPRVNGGANGTP-NRRLSLNQN 637

Query: 541 GSRSMNKDGKRDGMRPMTPVKYDAISKEDSGSHVSGTEPVSSSP 582
           GSRS  KD KR+  R   PV Y ++SKED+ SHVSGT+PV +SP
Sbjct: 638 GSRSAAKDAKREN-RLSAPVNYVSMSKEDAASHVSGTDPVPASP 679

BLAST of Carg25350-RA vs. TrEMBL
Match: tr|A0A2P5F268|A0A2P5F268_9ROSA (Microtubule-associated protein, MAP65/Ase1/PRC OS=Trema orientalis OX=63057 GN=TorRG33x02_122150 PE=4 SV=1)

HSP 1 Score: 904.0 bits (2335), Expect = 1.7e-259
Identity = 480/585 (82.05%), Postives = 522/585 (89.23%), Query Frame = 0

Query: 1   MAVEDTQNPLQGETTCGTLLQKLQEIWDEVGESDEDRDKMLLQIDQECLDVYKRKVELAD 60
           MAV D QNPL GETTCG+LLQKLQEIWDEVGE+DE+RDKMLLQ++QECLDVYKRKVE A 
Sbjct: 1   MAVTDAQNPLHGETTCGSLLQKLQEIWDEVGENDEERDKMLLQLEQECLDVYKRKVEQAA 60

Query: 61  KSRAKLLQSLSDAKHELSNLLSALGEKSFSEFPESASGTIKEQLAAIAPALEQLWKQKEK 120
           KSRA+LLQ+LSD K ELS LLSALG KS    PE  SGTIKEQLAAIAPALEQLWKQKE+
Sbjct: 61  KSRAQLLQALSDGKLELSGLLSALGGKSSVGIPEKTSGTIKEQLAAIAPALEQLWKQKEE 120

Query: 121 RVKEFFDVQSEIQKICGEITGSLNISEN---PVVDESQLSVEKLDEYHCQLQELQKEKSE 180
           RVKEF DVQS+IQKICGEI G+LN+S+N   P VDES L+++KLDEYH QLQELQKEKS+
Sbjct: 121 RVKEFSDVQSQIQKICGEIAGNLNVSDNVGTPTVDESDLTLKKLDEYHAQLQELQKEKSD 180

Query: 181 RLHKVLEFVSTVHDLCAVLGIDFFGTVTEVHPSLNDATGVQSKSISNDTLSKLAKMVLAL 240
           RLHKVLEFVS+VHDLCAVLG+DFF TVTEVHPSLND+TGVQSKSISNDTLS+LAK VLAL
Sbjct: 181 RLHKVLEFVSSVHDLCAVLGMDFFSTVTEVHPSLNDSTGVQSKSISNDTLSRLAKTVLAL 240

Query: 241 KEDKKQRLHKLQELATQLVDLWNLMDTPSEERSLFDHVTCNISASVDEVTVPGALALDLI 300
           KEDKKQRL KLQELA+QL+DLWNLMDTP EER LFDHVTCNISASVDEVTVPGALALDLI
Sbjct: 241 KEDKKQRLQKLQELASQLIDLWNLMDTPEEERELFDHVTCNISASVDEVTVPGALALDLI 300

Query: 301 EQAEVEVERLDQLKASRMKEIAFKKQSELEEIFAHAHIEIDPEAAREKIMTLIDSGNVEP 360
           EQAEVEVERLDQLK+SRMKEIAFK+Q+ELEEIF HAHIEIDPEAAR KIM +IDSGNVEP
Sbjct: 301 EQAEVEVERLDQLKSSRMKEIAFKRQTELEEIFVHAHIEIDPEAARAKIMAMIDSGNVEP 360

Query: 361 SELLDDMDNKIAKAKEEVLSRKEILDKVEKWMLACEEESWLEDYNRDDNRYNASRGAHLN 420
           +ELL DMDN+I KAK+E LSRKEILDKVEKWM ACEEESWLEDYNRDDNRYNASRGAHLN
Sbjct: 361 AELLADMDNQIVKAKDEALSRKEILDKVEKWMSACEEESWLEDYNRDDNRYNASRGAHLN 420

Query: 421 LKRAEKARILVNKIPALVDTLIAKTRAWEEDRGISFIYDGVPLLAMLDEYTMLRHEREEE 480
           LKRAEKARILVNKIPALV+TL+AKTRAWEEDRGISF YDGVPLLAMLDEY MLR EREEE
Sbjct: 421 LKRAEKARILVNKIPALVETLVAKTRAWEEDRGISFTYDGVPLLAMLDEYAMLRQEREEE 480

Query: 481 KRRFRDHKKFHEQQSTDQESITNSRPSPARPI-GTKKAAGPRANGAVNGTPSSRRLSLNQ 540
           KRR RD KK+ +QQ T+QE+I  S+PSPARP   TKK  GPRANG  NGTP SRRLSLNQ
Sbjct: 481 KRRLRDQKKYQDQQHTEQEAIFGSKPSPARPASSTKKVVGPRANGGANGTP-SRRLSLNQ 540

Query: 541 NGSRSMNKDGKRDGMRPMTPVKYDAISKEDSGSHVSGTEPVSSSP 582
           NGSRS  KD KR+  R   PV Y A+SKED+GSHVSGT+P+ +SP
Sbjct: 541 NGSRSATKDAKREN-RLSAPVNYVAMSKEDAGSHVSGTDPIPASP 583

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023005347.10.0e+0098.6365-kDa microtubule-associated protein 1-like [Cucurbita maxima][more]
XP_022942085.10.0e+0098.6265-kDa microtubule-associated protein 1-like [Cucurbita moschata][more]
XP_023520760.10.0e+0099.4865-kDa microtubule-associated protein 1-like [Cucurbita pepo subsp. pepo][more]
XP_011650516.15.4e-30293.98PREDICTED: 65-kDa microtubule-associated protein 1 [Cucumis sativus] >KGN56090.1... [more]
XP_022932234.19.2e-30293.8065-kDa microtubule-associated protein 1-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT5G55230.24.0e-21467.64microtubule-associated proteins 65-1[more]
AT4G26760.13.2e-19565.81microtubule-associated protein 65-2[more]
AT5G51600.13.8e-13249.20Microtubule associated protein (MAP65/ASE1) family protein[more]
AT2G38720.11.0e-12142.71microtubule-associated protein 65-5[more]
AT2G01910.13.0e-12143.41Microtubule associated protein (MAP65/ASE1) family protein[more]
Match NameE-valueIdentityDescription
sp|Q9FLP0|MA651_ARATH1.6e-21770.9765-kDa microtubule-associated protein 1 OS=Arabidopsis thaliana OX=3702 GN=MAP65... [more]
sp|Q8LEG3|MA652_ARATH5.7e-19465.8165-kDa microtubule-associated protein 2 OS=Arabidopsis thaliana OX=3702 GN=MAP65... [more]
sp|Q9FHM4|MA653_ARATH6.8e-13149.2065-kDa microtubule-associated protein 3 OS=Arabidopsis thaliana OX=3702 GN=MAP65... [more]
sp|Q9ZVJ3|MA655_ARATH1.5e-12545.7765-kDa microtubule-associated protein 5 OS=Arabidopsis thaliana OX=3702 GN=MAP65... [more]
sp|Q9SIS3|MA656_ARATH5.4e-12043.4165-kDa microtubule-associated protein 6 OS=Arabidopsis thaliana OX=3702 GN=MAP65... [more]
Match NameE-valueIdentityDescription
tr|A0A0A0L2B7|A0A0A0L2B7_CUCSA3.6e-30293.98Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G073820 PE=4 SV=1[more]
tr|A0A1S3BM91|A0A1S3BM91_CUCME2.5e-29592.6065-kDa microtubule-associated protein 1-like isoform X2 OS=Cucumis melo OX=3656 ... [more]
tr|A0A2P4H132|A0A2P4H132_QUESU7.8e-26582.9965-kDa microtubule-associated protein 1 OS=Quercus suber OX=58331 GN=CFP56_48531... [more]
tr|W9R8X5|W9R8X5_9ROSA2.0e-26082.36Uncharacterized protein OS=Morus notabilis OX=981085 GN=L484_012448 PE=4 SV=1[more]
tr|A0A2P5F268|A0A2P5F268_9ROSA1.7e-25982.05Microtubule-associated protein, MAP65/Ase1/PRC OS=Trema orientalis OX=63057 GN=T... [more]
The following terms have been associated with this mRNA:
Vocabulary: Biological Process
TermDefinition
GO:0000226microtubule cytoskeleton organization
Vocabulary: Molecular Function
TermDefinition
GO:0008017microtubule binding
Vocabulary: INTERPRO
TermDefinition
IPR007145MAP65_Ase1_PRC1
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000226 microtubule cytoskeleton organization
cellular_component GO:0045298 tubulin complex
molecular_function GO:0008017 microtubule binding

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Carg25350Carg25350gene


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Carg25350-RACarg25350-RA-proteinpolypeptide


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg25350-RA.exon.1Carg25350-RA.exon.1exon
Carg25350-RA.exon.2Carg25350-RA.exon.2exon
Carg25350-RA.exon.3Carg25350-RA.exon.3exon
Carg25350-RA.exon.4Carg25350-RA.exon.4exon
Carg25350-RA.exon.5Carg25350-RA.exon.5exon
Carg25350-RA.exon.6Carg25350-RA.exon.6exon
Carg25350-RA.exon.7Carg25350-RA.exon.7exon
Carg25350-RA.exon.8Carg25350-RA.exon.8exon
Carg25350-RA.exon.9Carg25350-RA.exon.9exon


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg25350-RA.five_prime_UTR.1Carg25350-RA.five_prime_UTR.1five_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg25350-RA.CDS.1Carg25350-RA.CDS.1CDS
Carg25350-RA.CDS.2Carg25350-RA.CDS.2CDS
Carg25350-RA.CDS.3Carg25350-RA.CDS.3CDS
Carg25350-RA.CDS.4Carg25350-RA.CDS.4CDS
Carg25350-RA.CDS.5Carg25350-RA.CDS.5CDS
Carg25350-RA.CDS.6Carg25350-RA.CDS.6CDS
Carg25350-RA.CDS.7Carg25350-RA.CDS.7CDS
Carg25350-RA.CDS.8Carg25350-RA.CDS.8CDS
Carg25350-RA.CDS.9Carg25350-RA.CDS.9CDS


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg25350-RA.three_prime_UTR.1Carg25350-RA.three_prime_UTR.1three_prime_UTR


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 297..317
NoneNo IPR availableCOILSCoilCoilcoord: 238..258
NoneNo IPR availableGENE3DG3DSA:1.20.58.1520coord: 368..501
e-value: 3.5E-27
score: 97.0
NoneNo IPR availablePFAMPF03999MAP65_ASE1coord: 53..541
e-value: 3.7E-91
score: 306.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 478..492
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 522..541
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 567..581
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 478..581
NoneNo IPR availablePANTHERPTHR19321:SF15SUBFAMILY NOT NAMEDcoord: 1..572
IPR007145Microtubule-associated protein, MAP65/Ase1/PRC1PANTHERPTHR19321PROTEIN REGULATOR OF CYTOKINESIS 1 PRC1-RELATEDcoord: 1..572