Carg23447-RA (mRNA) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg23447-RA
Type	mRNA
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	Calcium-binding EF hand family protein
Location	Cucurbita_argyrosperma_scaffold_335 : 232610 .. 236032 (+)
Sequence length	901

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

CGCGTTTGAAGTAAACTCTCCATTTCCCTCTCTCGAGCTTTTCTTCCGCTCTGCGAAAATGTCAGAAGCCACAATAACGATCCTGGACGGCACGCAGCTAAGAGCCATCGACCTCTCTCTGCTCTTCTCCGATCGTAGTGTCACAGGAGCTCAGGTCCTCGACCTTGCCGATTCCTCAGTCTCCTCGTCTCTGTTCGGGATCGCCTTGCCGGAGACTCTCAGATCATCTGCTCTTAATCGCATTGGCTTCCACGACATTGTTGCTTTCCGACGATCGGAGCTCACTCGTGAACGAGCCTCTGAGATTCTGAAGGCCTATGTAGCTGCGATCGCTGATGGTTTACGAGGTATCTTGTTTTCTCGTGTTTGTATGGTGTCTTAGTGCGTAATGTTCGTGAATTTCGTTATCGGAGCTTTTGGCAGCTCATCAAGAAGTACGATTGAGTGTTTTTTTTTAAAAAAAATCATGAATTTAACATTTTAGATCTTGCGAATAGTGAATTAGACTGTGAAGGATGAACAGAAAAGTAAGGAGAAATTGTTTCAGCATGATGAAATTGAACTGATGGATTAAATGTTTGTTGTTTAACTGGATTCCAAGCACGTGTAGGGCGTTGATTGCCAATGTGAACTAGATAATCCGCTCAAAAGTTATAATCTGATAATTTAGATATATATATATATACCTTGGTACAGATATCATGTATCTGTCTGGTTCAACCATTTTAGTCTAGTAGAATGATCGAGGATTACCTAGATAACTGTGTATTATGAACGATCTGTTGGATATATCTCTTTGTTTTATTTCTATAGCAAGAAAACGTTACTAATAAATTTTTGTTTTCCCAGATGATCCTGTTGTAGTGTCAATTTTGGATGGGAACAATCTGCGTGTTTTCTTAGATGATGAAGATGATTTTGCAATGCTAGCTGAGTCCCTTTTCACTGATTTAGATACAGAAGATAAAGGAAAGATCAAGAAGAGTGAGATTAAAAATGCTCTTATCCACATGGGGGTTGAAACGGGTGTACCACCTCTCTCAGGTTTATTCATATTTTACTTTCCTCCTTTTCTCCTTTTTTCTTGTTTAATTCTTGGTAATCTACATTCCATTTTTACTTTGGTCTATGTCTTTAGACTGAGAATTGTCCTGTACACGCTTCTACAACGAATTTATTTGCAAGTTGGTCTTCCTGCCTTTGTTTGTGGAATTTTTCAAAACTGTCTGCTCAATTATATCCACTGTTTCATGTGTTCAACATATCATGTTTAAAACTCCCGTGCATAAGCTTCTACAAGGGTATTTTGAATCTCCTCTTAGTGGTACAGTAGCTAGCTACATTATACTGGTACAAGTCTCTAACTATTTGCAGAAAAAAACATACTGCATCACATGTTTATCACGACTATGAATGCTTTCCACGTTAAATGGATGGATGAATTTCTAGAATGTTGTAATACATTAAAGAGATGCAGATCAGACTCATCAATGAGAAATTTTTGTATGAACATTCTCTTTGGATTTTCCTAACTTGGAAATGGTTTTTAAACACCTTTTCTCTACTATGACGTAAGGAACTTTAGTTTTGAGCATTTGTTCTATTTTTTAACTGATCAGTAAAAGGACCCTACTTCACATATGAAGTTATAACCTGTGTGCATTTGCAACGAACTTCTCTTTGTAACATACCAGTGATGAAACAGAATATCCTTTGCTGAGCGACATCTTGCAAAAACACGAAGTGGAGAGCAGCAATGAATTGGGCCAAGCTCAATTTGCAGAAGTGCTTCAGCCTGTCCTTCAAGAGTTAGCAGATACTCTTGCAAAAAAGCCTTATGTTTTCATTCAAAGTATCAAGGTCGCTAATGGAGCTCGGATAAAAAAGGTAAATCGGTACCTTGACTTTTCTTCCTTGGACATACCCTTCAATGATATCTTCCCCTTGACATTGGGAATTGGGAAGTGTTGTAAACCCTTTTTTTGTGAGAAACTTTGAAAGTGTAGTAAACCCTTAATACCATCATTAGCATATTTTCTTAGACAAGTTTACTGGCCTGGTTTATCTGCATCCGACATTTTACATACTAGAAGGATTACACCCATCAAATTGTGGATTCATTAATTTTGGAAATTTTAACATTATACTATCAGTAACAGGCACTGACAAAAAATGGGTAAGCCCCGACCTGTAGTTTCTTTTCCCCTTAATTTCTGCACTGTGTTGACTAGCTTCTGGATCTTAGAACTATCCACTGCACTTAATACCATGTGATATTGTTGTTATTATTTTATACTCGAGGAAAATATTCTTTCTGTTGGTCTTATGATGATAGATACCACTGAATGCAGTGAGCTAGTTCACTATTTTATCTTTCCTCCAACAACCTCCTGTCGACAATATCCTCTCGGTGATTTCCCTGGCCAATTATCGAAATTCAACAAGTGGTATCAGAGCCTCGTTGAAGCAATCGGTAGTATACCATCACAGAAGAGCCAACCTATCAACAATCAAGTTGAATAGCTCAAGTGGTTAGAGCGTCGACCTAACAACACACAGGTCTCAGGTTCGAGTCTCGGGGATCTCCTTTAACACAGAGGTAATCGACGACGGCATAACTGCTCGTCGATGAACGTCGAACCCGAGGAACTGCTCCCTGACGTCGAACCCGAGGAAGAACTCCCAAATCCACAGAGGTAATCGACGACGGCATAACTGCTCCGGTGAACGTCGAACCCGAGGAAGAACTCCAACTGTGCATAACAAATGCACCTGGGGAGCCAATTCGACTAAAGGAGGAGCGAGTGCTCGCTCAAATCGGTGAAAGAGGCAAGCAGCAAGAGCACCGGCGATGGGTCCTCGACACGGGGGCATCAAACCATATGACCGGGGCCAAGTCTGCGTTCTCTGAGCTTGACTCGGGGATTCGCGGGACGGTGAAATTTCGGCGATGGCTCCGTCGTCGAGATCGAAGGGCACGGTACCATCCTATTCGTCGGCAAGGGAGGCAATCATCACAAGCTGACCGCGTCTACTTCATCCCGATTTACGCATTTTATCTTTCCTCCAACAACCTCCTGTCGACAATATCCTCTCGGTGATTTCCCTGGCCAATTATCGAAATTCTACAATCTACTCTAGTGAACATTGACTTGCATGACTTCTTACTACTATTTGGGAACTATGAATACTTTTGAAAATATTGTAATTCCTGATGTGAGTTGCAGCTTTTGGCTGACGAGAAGCAATTCAGCAATGTCATAGAAAAGCTCCGGCAGTGGCAGGTTTGCTGATATAGCGAAGGAGAAGTGTGGTTCAATATCCGAAAAGAATCAAGTTGAAAAACTTGCAAAGGAGATTTTGGAGATATTTGTGGAGCAGCTTGAAGCCAGTCCTGTGTATTATGATTATGACCCCAAATGA

mRNA sequence

CGCGTTTGAAGTAAACTCTCCATTTCCCTCTCTCGAGCTTTTCTTCCGCTCTGCGAAAATGTCAGAAGCCACAATAACGATCCTGGACGGCACGCAGCTAAGAGCCATCGACCTCTCTCTGCTCTTCTCCGATCGTAGTGTCACAGGAGCTCAGGTCCTCGACCTTGCCGATTCCTCAGTCTCCTCGTCTCTGTTCGGGATCGCCTTGCCGGAGACTCTCAGATCATCTGCTCTTAATCGCATTGGCTTCCACGACATTGTTGCTTTCCGACGATCGGAGCTCACTCGTGAACGAGCCTCTGAGATTCTGAAGGCCTATGTAGCTGCGATCGCTGATGGTTTACGAGATGATCCTGTTGTAGTGTCAATTTTGGATGGGAACAATCTGCGTGTTTTCTTAGATGATGAAGATGATTTTGCAATGCTAGCTGAGTCCCTTTTCACTGATTTAGATACAGAAGATAAAGGAAAGATCAAGAAGAGTGAGATTAAAAATGCTCTTATCCACATGGGGGTTGAAACGGGTGTACCACCTCTCTCAGAATATCCTTTGCTGAGCGACATCTTGCAAAAACACGAAGTGGAGAGCAGCAATGAATTGGGCCAAGCTCAATTTGCAGAAGTGCTTCAGCCTGTCCTTCAAGAGTTAGCAGATACTCTTGCAAAAAAGCCTTATGTTTTCATTCAAAGTATCAAGGTCGCTAATGGAGCTCGGATAAAAAAGCTTTTGGCTGACGAGAAGCAATTCAGCAATGTCATAGAAAAGCTCCGGCAGTGGCAGGAGAAGTGTGGTTCAATATCCGAAAAGAATCAAGTTGAAAAACTTGCAAAGGAGATTTTGGAGATATTTGTGGAGCAGCTTGAAGCCAGTCCTGTGTATTATGATTATGACCCCAAATGA

Coding sequence (CDS)

ATGTCAGAAGCCACAATAACGATCCTGGACGGCACGCAGCTAAGAGCCATCGACCTCTCTCTGCTCTTCTCCGATCGTAGTGTCACAGGAGCTCAGGTCCTCGACCTTGCCGATTCCTCAGTCTCCTCGTCTCTGTTCGGGATCGCCTTGCCGGAGACTCTCAGATCATCTGCTCTTAATCGCATTGGCTTCCACGACATTGTTGCTTTCCGACGATCGGAGCTCACTCGTGAACGAGCCTCTGAGATTCTGAAGGCCTATGTAGCTGCGATCGCTGATGGTTTACGAGATGATCCTGTTGTAGTGTCAATTTTGGATGGGAACAATCTGCGTGTTTTCTTAGATGATGAAGATGATTTTGCAATGCTAGCTGAGTCCCTTTTCACTGATTTAGATACAGAAGATAAAGGAAAGATCAAGAAGAGTGAGATTAAAAATGCTCTTATCCACATGGGGGTTGAAACGGGTGTACCACCTCTCTCAGAATATCCTTTGCTGAGCGACATCTTGCAAAAACACGAAGTGGAGAGCAGCAATGAATTGGGCCAAGCTCAATTTGCAGAAGTGCTTCAGCCTGTCCTTCAAGAGTTAGCAGATACTCTTGCAAAAAAGCCTTATGTTTTCATTCAAAGTATCAAGGTCGCTAATGGAGCTCGGATAAAAAAGCTTTTGGCTGACGAGAAGCAATTCAGCAATGTCATAGAAAAGCTCCGGCAGTGGCAGGAGAAGTGTGGTTCAATATCCGAAAAGAATCAAGTTGAAAAACTTGCAAAGGAGATTTTGGAGATATTTGTGGAGCAGCTTGAAGCCAGTCCTGTGTATTATGATTATGACCCCAAATGA

Protein sequence

MSEATITILDGTQLRAIDLSLLFSDRSVTGAQVLDLADSSVSSSLFGIALPETLRSSALNRIGFHDIVAFRRSELTRERASEILKAYVAAIADGLRDDPVVVSILDGNNLRVFLDDEDDFAMLAESLFTDLDTEDKGKIKKSEIKNALIHMGVETGVPPLSEYPLLSDILQKHEVESSNELGQAQFAEVLQPVLQELADTLAKKPYVFIQSIKVANGARIKKLLADEKQFSNVIEKLRQWQEKCGSISEKNQVEKLAKEILEIFVEQLEASPVYYDYDPK

Homology

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BLAST of Carg23447-RA vs. NCBI nr
Match: XP_022946830.1 (uncharacterized protein LOC111450774 [Cucurbita moschata])

HSP 1 Score: 498.8 bits (1283), Expect = 1.2e-137
Identity = 278/330 (84.24%), Postives = 279/330 (84.55%), Query Frame = 0


Query: 1   MSEATITILDGTQLRAIDLSLLFSDRSVTGAQVLDLADSSVSSSLFGIALPETLRSSALN 60
           MSEATITILDGTQLR+IDLSLLFSDRSVTGAQVLDLADSSVSSSLFGIALPETLRSSALN
Sbjct: 1   MSEATITILDGTQLRSIDLSLLFSDRSVTGAQVLDLADSSVSSSLFGIALPETLRSSALN 60

Query: 61  RIGFHDIVAFRRSELTRERASEILKAYVAAIADGLRDDPVVVSILDGNNLRVFLDDEDDF 120
           RIGFHDIVAFRRSELTRERASEILKAYVAAIADGLRDDPVVVSILDGNNLRVFLDDEDDF
Sbjct: 61  RIGFHDIVAFRRSELTRERASEILKAYVAAIADGLRDDPVVVSILDGNNLRVFLDDEDDF 120

Query: 121 AMLAESLFTDLDTEDKGKIKKSEIKNALIHMGVETGVPPLSEYPLLSDILQKHEVESSNE 180
           AMLAESLFTDLDTEDKGKIKKSEIKNALIHMGVETGVPPLSEYPLLSDILQKHEVESSNE
Sbjct: 121 AMLAESLFTDLDTEDKGKIKKSEIKNALIHMGVETGVPPLSEYPLLSDILQKHEVESSNE 180

Query: 181 LGQAQFAEVLQPVLQELADTLAKKPYVFIQSIKVANGARIKKLLADEKQFSNVIEKLRQW 240
           LGQAQFAEVLQPVLQELADTLAKKPYVFIQSIKVANGARIKKLLADEKQFSNVIEKL QW
Sbjct: 181 LGQAQFAEVLQPVLQELADTLAKKPYVFIQSIKVANGARIKKLLADEKQFSNVIEKLWQW 240

Query: 241 Q--------------------------------------------------EKCGSISEK 281
           Q                                                  EKCGSISEK
Sbjct: 241 QGTHKEEDEVTTSQNIRNYFEKEWRELGLPPTEANDAVVLLYDAIFADIAKEKCGSISEK 300

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BLAST of Carg23447-RA vs. NCBI nr
Match: XP_023547272.1 (uncharacterized protein LOC111806141 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 483.0 bits (1242), Expect = 6.7e-133
Identity = 270/330 (81.82%), Postives = 275/330 (83.33%), Query Frame = 0


Query: 1   MSEATITILDGTQLRAIDLSLLFSDRSVTGAQVLDLADSSVSSSLFGIALPETLRSSALN 60
           MSEATITILDGTQLR+IDLSL FSDRSVTGAQVLDLADSSVSSSLFGIAL ETLRSSALN
Sbjct: 1   MSEATITILDGTQLRSIDLSLSFSDRSVTGAQVLDLADSSVSSSLFGIALSETLRSSALN 60

Query: 61  RIGFHDIVAFRRSELTRERASEILKAYVAAIADGLRDDPVVVSILDGNNLRVFLDDEDDF 120
           RIGFHDIVAFRRSELTRERASEILK YVAAIADGLRDDPVVVSILDGNNLRVFLDDEDDF
Sbjct: 61  RIGFHDIVAFRRSELTRERASEILKDYVAAIADGLRDDPVVVSILDGNNLRVFLDDEDDF 120

Query: 121 AMLAESLFTDLDTEDKGKIKKSEIKNALIHMGVETGVPPLSEYPLLSDILQKHEVESSNE 180
           AMLAE+LFTDLDTEDKGKIKKSE+KNALIHMGVETGVPPLSEYPLLSDILQKHEVESSNE
Sbjct: 121 AMLAETLFTDLDTEDKGKIKKSEVKNALIHMGVETGVPPLSEYPLLSDILQKHEVESSNE 180

Query: 181 LGQAQFAEVLQPVLQELADTLAKKPYVFIQSIKVANGARIKKLLADEKQFSNVIEKLRQW 240
           LGQAQFAEVLQPVLQELADTLAKKPYVFI+SIKVANGA+IKKLLADEKQFSNVIEKL QW
Sbjct: 181 LGQAQFAEVLQPVLQELADTLAKKPYVFIRSIKVANGAQIKKLLADEKQFSNVIEKLWQW 240

Query: 241 Q--------------------------------------------------EKCGSISEK 281
           Q                                                  EKCGSISEK
Sbjct: 241 QGTHKEEDEVTTLQKIRNYFEKEWRELGLPPTEANDAMVLLYDAIFADIAKEKCGSISEK 300

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BLAST of Carg23447-RA vs. NCBI nr
Match: XP_022999409.1 (uncharacterized protein LOC111493790 [Cucurbita maxima])

HSP 1 Score: 480.7 bits (1236), Expect = 3.3e-132
Identity = 271/330 (82.12%), Postives = 273/330 (82.73%), Query Frame = 0


Query: 1   MSEATITILDGTQLRAIDLSLLFSDRSVTGAQVLDLADSSVSSSLFGIALPETLRSSALN 60
           MSE TITILDGTQLR+IDLSL FSD SV+GAQVLDLADSSVSSSLF IAL ETLRSSALN
Sbjct: 1   MSETTITILDGTQLRSIDLSLPFSDGSVSGAQVLDLADSSVSSSLFEIALSETLRSSALN 60

Query: 61  RIGFHDIVAFRRSELTRERASEILKAYVAAIADGLRDDPVVVSILDGNNLRVFLDDEDDF 120
           RIGFHDIVAFRRSELTRERASEILKAYVAAIADGLRDDPVVVSILDGNNLRVFLDDEDDF
Sbjct: 61  RIGFHDIVAFRRSELTRERASEILKAYVAAIADGLRDDPVVVSILDGNNLRVFLDDEDDF 120

Query: 121 AMLAESLFTDLDTEDKGKIKKSEIKNALIHMGVETGVPPLSEYPLLSDILQKHEVESSNE 180
           AMLAESLFTDLDTEDKGKIKKSEIKNALIHMGVETGVPPLSEYPLLSDILQKHEVESSNE
Sbjct: 121 AMLAESLFTDLDTEDKGKIKKSEIKNALIHMGVETGVPPLSEYPLLSDILQKHEVESSNE 180

Query: 181 LGQAQFAEVLQPVLQELADTLAKKPYVFIQSIKVANGARIKKLLADEKQFSNVIEKLRQW 240
           LGQAQFAEVLQPVLQELADTLAKKPYVFIQSIKVANGARIKKLLADEKQFSNVIEKL QW
Sbjct: 181 LGQAQFAEVLQPVLQELADTLAKKPYVFIQSIKVANGARIKKLLADEKQFSNVIEKLWQW 240

Query: 241 Q--------------------------------------------------EKCGSISEK 281
           Q                                                  EKCGSISEK
Sbjct: 241 QGTHKEEDEVTTSQKIRNYFEKEWRELGLPPTEANDAVVLLYDAIFADIAKEKCGSISEK 300

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BLAST of Carg23447-RA vs. NCBI nr
Match: XP_004139231.1 (PREDICTED: uncharacterized protein LOC101222315 [Cucumis sativus] >KGN60814.1 hypothetical protein Csa_2G011470 [Cucumis sativus])

HSP 1 Score: 412.1 bits (1058), Expect = 1.5e-111
Identity = 228/327 (69.72%), Postives = 252/327 (77.06%), Query Frame = 0


Query: 1   MSEATITILDGTQLRAIDLSLLFSDRSVTGAQVLDLADSSVSSSLFGIALPETLRSSALN 60
           M+E  IT+LDGT LR IDL+  FSD  +TGA +LDLADS+ SSSLFGI LPETL+SSALN
Sbjct: 1   MAEGPITVLDGTHLRDIDLTPPFSDSVLTGAHLLDLADSTASSSLFGIPLPETLKSSALN 60

Query: 61  RIGFHDIVAFRRSELTRERASEILKAYVAAIADGLRDDPVVVSILDGNNLRVFLDDEDDF 120
            IG HDIVAFRRSELT +RAS+ILK YV+AIAD LRDDP++VSILDGN LR+FLDDEDDF
Sbjct: 61  IIGLHDIVAFRRSELTSQRASQILKDYVSAIADILRDDPLLVSILDGNTLRIFLDDEDDF 120

Query: 121 AMLAESLFTDLDTEDKGKIKKSEIKNALIHMGVETGVPPLSEYPLLSDILQKHEVESSNE 180
           AMLAE+LFTDLDTEDKGKIKKSEIKNAL+HMGVETGVPPLSEYPLLSDILQKHEVESS E
Sbjct: 121 AMLAETLFTDLDTEDKGKIKKSEIKNALVHMGVETGVPPLSEYPLLSDILQKHEVESSTE 180

Query: 181 LGQAQFAEVLQPVLQELADTLAKKPYVFIQSIKVANGARIKKLLADEKQFSNVIEKLRQW 240
           LGQAQ+AEVLQ VLQELAD LAKKPYVFIQ+IK+ NGA++KKLL DEKQF++VIEKL QW
Sbjct: 181 LGQAQYAEVLQAVLQELADALAKKPYVFIQNIKITNGAQVKKLLTDEKQFNDVIEKLWQW 240

Query: 241 Q---------------------------------------------------EKCGSISE 277
           Q                                                   EKC SIS+
Sbjct: 241 QGTHKEENGVTTLQKIRNYFEKEWKELGLPPPTETNEAIVLLYDEIFADIAKEKCDSISD 300

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BLAST of Carg23447-RA vs. NCBI nr
Match: XP_022142975.1 (uncharacterized protein LOC111012973 [Momordica charantia])

HSP 1 Score: 406.4 bits (1043), Expect = 8.0e-110
Identity = 224/328 (68.29%), Postives = 252/328 (76.83%), Query Frame = 0


Query: 1   MSEATITILDGTQLRAIDLSLLFSDRSVTGAQVLDLADSSVSSSLFGIALPETLRSSALN 60
           MSEA IT+LDGT L+ IDLSL F D  +TGAQ+L LADS  SSSLFG+ALPETLRSSAL+
Sbjct: 1   MSEAAITVLDGTHLQHIDLSLPFPDGGLTGAQLLHLADSKASSSLFGLALPETLRSSALS 60

Query: 61  RIGFHDIVAFRRSELTRERASEILKAYVAAIADGLRDDPVVVSILDGNNLRVFLDDEDDF 120
           RIG  DIVAFRRSELT ERASEIL+ YV+AIADGL +DP+VVSILDGN LRVFLDDEDDF
Sbjct: 61  RIGLPDIVAFRRSELTPERASEILRGYVSAIADGLGEDPLVVSILDGNTLRVFLDDEDDF 120

Query: 121 AMLAESLFTDLDTEDKGKIKKSEIKNALIHMGVETGVPPLSEYPLLSDILQKHEVESSNE 180
           AM+AE+LFTDLDTEDKGKI+KSEI+NAL+HMG E GVPPLSEYPLLSDILQKH+V+S+ E
Sbjct: 121 AMIAENLFTDLDTEDKGKIRKSEIQNALVHMGFEMGVPPLSEYPLLSDILQKHDVKSNKE 180

Query: 181 LGQAQFAEVLQPVLQELADTLAKKPYVFIQSIKVANGARIKKLLADEKQFSNVIEKL--- 240
           LGQAQFAEVLQPVLQELAD LAKKPYVFIQ++K+ANGA+IKKLLADEK F+NVIEKL   
Sbjct: 181 LGQAQFAEVLQPVLQELADALAKKPYVFIQNLKIANGAQIKKLLADEKHFNNVIEKLWQE 240

Query: 241 -------------------RQWQ--------------------------EKCGSISEKNQ 281
                              ++W+                          E CGSIS+KNQ
Sbjct: 241 THKAEDGITTLQKIRNYFEKEWKELGLPPTEANEAVVLLYDAVFADTANENCGSISDKNQ 300

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BLAST of Carg23447-RA vs. TAIR10
Match: AT5G28830.1 (calcium-binding EF hand family protein)

HSP 1 Score: 255.4 bits (651), Expect = 4.1e-68
Identity = 128/233 (54.94%), Postives = 179/233 (76.82%), Query Frame = 0


Query: 4   ATITILDGTQLRAIDLSLLFSDRSVTGAQVLDLADSSVSSSLFGIALPETLRSSALNRIG 63
           A +TI DG  LR+IDL+L      VTGAQ+L++++S VS SL G++LP  L+ +A++++ 
Sbjct: 5   AGLTIFDGDLLRSIDLNLPEHQHRVTGAQLLEISESKVSQSLSGLSLPPHLKETAISQVS 64

Query: 64  FHDIVAFRRSELTRERASEILKAYVAAIADGLRDDPVVVSILDGNNLRVFLDDEDDFAML 123
             D V FRR+   +++ASE L  + + +AD L+D P+VVSILDG  L++FL+DEDDFAML
Sbjct: 65  DGDHVTFRRTMFNKQQASEKLGVFFSTVADALKDTPIVVSILDGTMLKMFLEDEDDFAML 124

Query: 124 AESLFTDLDTEDKGKIKKSEIKNALIHMGVETGVPPLSEYPLLSDILQKHEVESSNELGQ 183
           AE+LFTDLD EDKGK+ KSEI+ AL+HMGVE GVPPLSE+P+L DI++KH+ +S  ELGQ
Sbjct: 125 AENLFTDLDEEDKGKLCKSEIRKALVHMGVEMGVPPLSEFPILDDIIKKHDADSDEELGQ 184

Query: 184 AQFAEVLQPVLQELADTLAKKPYVFIQSIKVANGARIKKLLADEKQFSNVIEK 237
           AQFAE+LQ VLQE+AD L +KP   + ++++  G+RI+K+LADEK    ++EK
Sbjct: 185 AQFAELLQQVLQEIADVLHEKPITIVLNVEIFTGSRIRKILADEKTLKCLVEK 237

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BLAST of Carg23447-RA vs. TAIR10
Match: AT4G38810.2 (Calcium-binding EF-hand family protein)

HSP 1 Score: 52.0 bits (123), Expect = 7.0e-07
Identity = 29/111 (26.13%), Postives = 58/111 (52.25%), Query Frame = 0


Query: 104 ILDGNNLRVFLDDEDDFAMLAESLFTDLDTEDKGKIKKSEIKNALIHMGVETGVPPLSEY 163
           +LDG+++   +++E  F    E  F  LD ++ GK+  +E++ A+  +G   G+P     
Sbjct: 14  VLDGSDIVELVENEKVFDKFVEQKFQQLDQDEDGKLSVTELQPAVADIGAALGLPAQGTS 73

Query: 164 P----LLSDILQKHEVESSNELGQAQFAEVLQPVLQELADTLAKKPYVFIQ 211
           P    + S++L +    S  ++ + +F EVL  +L  +A  L + P V ++
Sbjct: 74  PDSDHIYSEVLNEFTHGSQEKVSKTEFKEVLSDILLGMAAGLKRDPIVILR 124

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BLAST of Carg23447-RA vs. TAIR10
Match: AT2G44310.1 (Calcium-binding EF-hand family protein)

HSP 1 Score: 50.8 bits (120), Expect = 1.6e-06
Identity = 29/115 (25.22%), Postives = 52/115 (45.22%), Query Frame = 0


Query: 102 VSILDGNNLRVFLDDEDDFAMLAESLFTDLDTEDKGKIKKSEIKNAL-------IHMGVE 161
           V ++DG+ +R F+DDE+ F    +  F  LD    G + +SE++ A         H GV+
Sbjct: 3   VVLIDGSTVRSFVDDEEQFKKSVDERFAALDLNKDGVLSRSELRKAFESMRLLESHFGVD 62

Query: 162 TGVPPLSEYPLLSDILQKHEVESSNELGQAQFAEVLQPVLQELADTLAKKPYVFI 210
              P      L   I +K + + S  +   +F   ++ ++  +AD L   P   +
Sbjct: 63  VVTPQDELTNLYDSIFEKFDTDQSGSVDLEEFRSEMKKIVLAIADGLGSCPITMV 117

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BLAST of Carg23447-RA vs. TrEMBL
Match: tr|A0A0A0LID8|A0A0A0LID8_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G011470 PE=4 SV=1)

HSP 1 Score: 412.1 bits (1058), Expect = 9.7e-112
Identity = 228/327 (69.72%), Postives = 252/327 (77.06%), Query Frame = 0


Query: 1   MSEATITILDGTQLRAIDLSLLFSDRSVTGAQVLDLADSSVSSSLFGIALPETLRSSALN 60
           M+E  IT+LDGT LR IDL+  FSD  +TGA +LDLADS+ SSSLFGI LPETL+SSALN
Sbjct: 1   MAEGPITVLDGTHLRDIDLTPPFSDSVLTGAHLLDLADSTASSSLFGIPLPETLKSSALN 60

Query: 61  RIGFHDIVAFRRSELTRERASEILKAYVAAIADGLRDDPVVVSILDGNNLRVFLDDEDDF 120
            IG HDIVAFRRSELT +RAS+ILK YV+AIAD LRDDP++VSILDGN LR+FLDDEDDF
Sbjct: 61  IIGLHDIVAFRRSELTSQRASQILKDYVSAIADILRDDPLLVSILDGNTLRIFLDDEDDF 120

Query: 121 AMLAESLFTDLDTEDKGKIKKSEIKNALIHMGVETGVPPLSEYPLLSDILQKHEVESSNE 180
           AMLAE+LFTDLDTEDKGKIKKSEIKNAL+HMGVETGVPPLSEYPLLSDILQKHEVESS E
Sbjct: 121 AMLAETLFTDLDTEDKGKIKKSEIKNALVHMGVETGVPPLSEYPLLSDILQKHEVESSTE 180

Query: 181 LGQAQFAEVLQPVLQELADTLAKKPYVFIQSIKVANGARIKKLLADEKQFSNVIEKLRQW 240
           LGQAQ+AEVLQ VLQELAD LAKKPYVFIQ+IK+ NGA++KKLL DEKQF++VIEKL QW
Sbjct: 181 LGQAQYAEVLQAVLQELADALAKKPYVFIQNIKITNGAQVKKLLTDEKQFNDVIEKLWQW 240

Query: 241 Q---------------------------------------------------EKCGSISE 277
           Q                                                   EKC SIS+
Sbjct: 241 QGTHKEENGVTTLQKIRNYFEKEWKELGLPPPTETNEAIVLLYDEIFADIAKEKCDSISD 300

[Close]

BLAST of Carg23447-RA vs. TrEMBL
Match: tr|A0A1S3C1Z5|A0A1S3C1Z5_CUCME (uncharacterized protein LOC103495980 OS=Cucumis melo OX=3656 GN=LOC103495980 PE=4 SV=1)

HSP 1 Score: 402.9 bits (1034), Expect = 5.9e-109
Identity = 227/327 (69.42%), Postives = 250/327 (76.45%), Query Frame = 0


Query: 1   MSEATITILDGTQLRAIDLSLLFSDRSVTGAQVLDLADSSVSSSLFGIALPETLRSSALN 60
           MSE  IT+LDGT LR IDL+  FSD  +TGA +LDLADS+VSSSLF I LPE L+SSALN
Sbjct: 1   MSEGPITVLDGTHLRDIDLTPHFSDALLTGAHLLDLADSTVSSSLFRIPLPEALKSSALN 60

Query: 61  RIGFHDIVAFRRSELTRERASEILKAYVAAIADGLRDDPVVVSILDGNNLRVFLDDEDDF 120
            IG HDIVAFRRSELT +RASEILK YV+AIAD LRDDP++VSILDG+ LR+FLDDEDDF
Sbjct: 61  IIGLHDIVAFRRSELTPQRASEILKDYVSAIADILRDDPLLVSILDGSTLRIFLDDEDDF 120

Query: 121 AMLAESLFTDLDTEDKGKIKKSEIKNALIHMGVETGVPPLSEYPLLSDILQKHEVESSNE 180
           AMLAE+LFTDLDTEDKGKIKKSEIKNAL+HMGVETGVPPLSEYPLL DILQKHEVESS E
Sbjct: 121 AMLAETLFTDLDTEDKGKIKKSEIKNALVHMGVETGVPPLSEYPLLRDILQKHEVESSTE 180

Query: 181 LGQAQFAEVLQPVLQELADTLAKKPYVFIQSIKVANGARIKKLLADEKQFSNVIEKLRQW 240
           LGQAQFAEVLQ VLQELAD LAKKPYVFIQ+IK+ NGA+IKKLLAD+KQF++VIEKL QW
Sbjct: 181 LGQAQFAEVLQAVLQELADALAKKPYVFIQNIKITNGAQIKKLLADKKQFNDVIEKLWQW 240

Query: 241 Q---------------------------------------------------EKCGSISE 277
           Q                                                   EKC SI++
Sbjct: 241 QGTYKEENGVTTLQKIRNYFEKEWKELGLPPPTETNEAVVLLYDEIFADIAKEKCCSITD 300

[Close]

BLAST of Carg23447-RA vs. TrEMBL
Match: tr|A0A251P1E3|A0A251P1E3_PRUPE (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_6G342700 PE=4 SV=1)

HSP 1 Score: 313.5 bits (802), Expect = 4.7e-82
Identity = 173/312 (55.45%), Postives = 224/312 (71.79%), Query Frame = 0


Query: 1   MSEATITILDGTQLRAIDLSLLFSDRSVTGAQVLDLADSSVSSSLFGIALPETLRSSALN 60
           MS+  +T+LDG  LRAIDLSL  ++ S+TGAQVLDLADS  SSSLFG++LP++L+SSAL 
Sbjct: 1   MSDGALTVLDGNHLRAIDLSLPEAEVSLTGAQVLDLADSKASSSLFGLSLPQSLKSSALK 60

Query: 61  RIGFHDIVAFRRSELTRERASEILKAYVAAIADGLRDDPVVVSILDGNNLRVFLDDEDDF 120
           RI   D   FR  EL RE+A +++  Y+ AIAD L+DDP+V+S+LDG  LR+FL+DEDDF
Sbjct: 61  RISLQDDDVFRLKELDREQALKVITDYITAIADELKDDPLVISVLDGYTLRLFLEDEDDF 120

Query: 121 AMLAESLFTDLDTEDKGKIKKSEIKNALIHMGVETGVPPLSEYPLLSDILQKHEVESSNE 180
           AMLAE+LFTDLD ED GKI K+EI+NAL+HMGVE GVPP+SE+P LSDIL+KH+ +   E
Sbjct: 121 AMLAENLFTDLDVEDTGKINKNEIRNALVHMGVEMGVPPISEFPPLSDILKKHKADGEEE 180

Query: 181 LGQAQFAEVLQPVLQELADTLAKKPYVFIQSIKVANGARIKKLLADEKQFSNVI------ 240
           LGQAQFAE+LQPVLQEL++ LAKK +V IQ+IK+ NG++++KLLADEKQ + ++      
Sbjct: 181 LGQAQFAELLQPVLQELSEALAKKHFVTIQNIKIVNGSKLRKLLADEKQLNIIVEKILAD 240

Query: 241 -----EKLRQWQEKCGS-----ISEKNQV--------------------EKLAKEILEIF 277
                EK+R + EK G+      SE N+                       L K+ILE F
Sbjct: 241 GSGNTEKIRSFLEKTGTELGLPPSEANEAVALLYDAVFADLEEAGEDKFGNLVKQILEKF 300

[Close]

BLAST of Carg23447-RA vs. TrEMBL
Match: tr|M1BPP2|M1BPP2_SOLTU (Uncharacterized protein OS=Solanum tuberosum OX=4113 GN=102584392 PE=4 SV=1)

HSP 1 Score: 308.9 bits (790), Expect = 1.2e-80
Identity = 172/324 (53.09%), Postives = 224/324 (69.14%), Query Frame = 0


Query: 1   MSEATITILDGTQLRAIDLSLLF-SDRSVTGAQVLDLADSSVSSSLFGIALPETLRSSAL 60
           MS+  +TILDGTQLRA+DLSL   +D SVTGAQ+LD+A+S VS SLFG +LP+TL+S+AL
Sbjct: 1   MSDGGLTILDGTQLRAVDLSLPSPADSSVTGAQLLDVAESKVSESLFGFSLPDTLKSAAL 60

Query: 61  NRIGFHDIVAFRRSELTRERASEILKAYVAAIADGLRDDPVVVSILDGNNLRVFLDDEDD 120
            RIG  D + FRR +L RE A  +L+ YV AIA+ L+DDP+V++ILDG  L++FL+DEDD
Sbjct: 61  KRIGVSDDLEFRREQLDRENALSVLRNYVDAIANELQDDPIVIAILDGKTLQMFLEDEDD 120

Query: 121 FAMLAESLFTDLDTEDKGKIKKSEIKNALIHMGVETGVPPLSEYPLLSDILQKHEVESSN 180
           FAMLAE+LFTDLDTEDKGKIK+++I++AL HMGVE G+PPLSE+PLLSDIL++H  E  +
Sbjct: 121 FAMLAENLFTDLDTEDKGKIKRNQIRDALFHMGVEMGIPPLSEFPLLSDILKRHGAEGED 180

Query: 181 ELGQAQFAEVLQPVLQELADTLAKKPYVFIQSIKVANGARIKKLLADEKQFSNVIEK--- 240
           ELGQAQFA +LQPVLQELAD LA+ P V +Q IK+ NG++++K+LADEKQ S  +EK   
Sbjct: 181 ELGQAQFAHLLQPVLQELADVLAENPMVVLQKIKINNGSKLRKILADEKQLSETVEKIMQ 240

Query: 241 -----------LRQWQEKCGS-------------------------------ISEKNQVE 279
                      +R + EK G+                                SEK++  
Sbjct: 241 EEKDGLSTKDVIRHYLEKNGASLGLPPLNDEVVILLYDTVLGAIENGNTDAKTSEKDEFL 300

[Close]

BLAST of Carg23447-RA vs. TrEMBL
Match: tr|A0A2P6R5Z0|A0A2P6R5Z0_ROSCH (Uncharacterized protein OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr3g0451171 PE=4 SV=1)

HSP 1 Score: 305.4 bits (781), Expect = 1.3e-79
Identity = 172/321 (53.58%), Postives = 217/321 (67.60%), Query Frame = 0


Query: 1   MSEATITILDGTQLRAIDLSLLFSDRSVTGAQVLDLADSSVSSSLFGIALPETLRSSALN 60
           MS+  +T+LDGT LRA+DLSL  SD  +TG  VLDLADS  SSSLFG++LP+ L+SSAL 
Sbjct: 1   MSDGALTVLDGTHLRAVDLSLPDSDVILTGGHVLDLADSKASSSLFGLSLPQGLKSSALK 60

Query: 61  RIGFHDIVAFRRSELTRERASEILKAYVAAIADGLRDDPVVVSILDGNNLRVFLDDEDDF 120
           R+   D   FR++EL R +A ++   Y+AAIAD L+DDP+V+SILDGN +R+FL+DEDDF
Sbjct: 61  RVNVQDDDVFRQAELDRHQALKLAGDYIAAIADELKDDPLVISILDGNTIRLFLEDEDDF 120

Query: 121 AMLAESLFTDLDTEDKGKIKKSEIKNALIHMGVETGVPPLSEYPLLSDILQKHEVESSNE 180
           AMLAE+LFTDLD ED GKI KS I++AL+HMGVE GVPP SE+P LSDIL KHE     E
Sbjct: 121 AMLAENLFTDLDAEDVGKINKSAIRSALVHMGVEMGVPPASEFPPLSDILTKHEANGEEE 180

Query: 181 LGQAQFAEVLQPVLQELADTLAKKPYVFIQSIKVANGARIKKLLADEKQFSNVIEKLRQW 240
           LGQAQFAE+LQP+LQELA+ LAKK  V IQ+IK+ NG++++KLLADEKQ +NV+EK+ Q 
Sbjct: 181 LGQAQFAELLQPILQELAEALAKKNVVLIQNIKIVNGSKLRKLLADEKQLNNVVEKILQG 240

Query: 241 Q-------EKCGSISEKN--------------------------------------QVEK 277
           +       E   S  EKN                                      + E 
Sbjct: 241 KKDDLGSAEVIRSYLEKNGLELGLPPSEGDEAVAILYDLVFAELKEDKSALKEGEDKFEN 300

The following BLAST results are available for this feature:

BLAST of Carg23447-RA vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022946830.1	1.2e-137	84.24	uncharacterized protein LOC111450774 [Cucurbita moschata]	[more]
XP_023547272.1	6.7e-133	81.82	uncharacterized protein LOC111806141 [Cucurbita pepo subsp. pepo]	[more]
XP_022999409.1	3.3e-132	82.12	uncharacterized protein LOC111493790 [Cucurbita maxima]	[more]
XP_004139231.1	1.5e-111	69.72	PREDICTED: uncharacterized protein LOC101222315 [Cucumis sativus] >KGN60814.1 hy...	[more]
XP_022142975.1	8.0e-110	68.29	uncharacterized protein LOC111012973 [Momordica charantia]	[more]

BLAST of Carg23447-RA vs. TAIR10


Match Name	E-value	Identity	Description
AT5G28830.1	4.1e-68	54.94	calcium-binding EF hand family protein	[more]
AT4G38810.2	7.0e-07	26.13	Calcium-binding EF-hand family protein	[more]
AT2G44310.1	1.6e-06	25.22	Calcium-binding EF-hand family protein	[more]

BLAST of Carg23447-RA vs. Swiss-Prot


Match Name	E-value	Identity	Description

BLAST of Carg23447-RA vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A0A0LID8\|A0A0A0LID8_CUCSA	9.7e-112	69.72	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G011470 PE=4 SV=1	[more]
tr\|A0A1S3C1Z5\|A0A1S3C1Z5_CUCME	5.9e-109	69.42	uncharacterized protein LOC103495980 OS=Cucumis melo OX=3656 GN=LOC103495980 PE=...	[more]
tr\|A0A251P1E3\|A0A251P1E3_PRUPE	4.7e-82	55.45	Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_6G342700 PE=4 SV=1	[more]
tr\|M1BPP2\|M1BPP2_SOLTU	1.2e-80	53.09	Uncharacterized protein OS=Solanum tuberosum OX=4113 GN=102584392 PE=4 SV=1	[more]
tr\|A0A2P6R5Z0\|A0A2P6R5Z0_ROSCH	1.3e-79	53.58	Uncharacterized protein OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr3g0451171 PE=4...	[more]

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003674	molecular_function

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Carg23447	Carg23447	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Carg23447-RA	Carg23447-RA-protein	polypeptide

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg23447-RA.exon.1	Carg23447-RA.exon.1	exon
Carg23447-RA.exon.2	Carg23447-RA.exon.2	exon
Carg23447-RA.exon.3	Carg23447-RA.exon.3	exon
Carg23447-RA.exon.4	Carg23447-RA.exon.4	exon
Carg23447-RA.exon.5	Carg23447-RA.exon.5	exon

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg23447-RA.five_prime_UTR.1	Carg23447-RA.five_prime_UTR.1	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg23447-RA.CDS.1	Carg23447-RA.CDS.1	CDS
Carg23447-RA.CDS.2	Carg23447-RA.CDS.2	CDS
Carg23447-RA.CDS.3	Carg23447-RA.CDS.3	CDS
Carg23447-RA.CDS.4	Carg23447-RA.CDS.4	CDS
Carg23447-RA.CDS.5	Carg23447-RA.CDS.5	CDS

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR34574	FAMILY NOT NAMED	coord: 1..243
None	No IPR available	PANTHER	PTHR34574:SF3	SUBFAMILY NOT NAMED	coord: 1..243