Carg23210-RA (mRNA) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg23210-RA
Type	mRNA
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	L-lactate dehydrogenase
Location	Cucurbita_argyrosperma_scaffold_144 : 383277 .. 384973 (+)
Sequence length	1053

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGAAGAGTTCATCAGCATCTTCCTTAGGTCCAGGCGGCCTTGACATTTCGCAGGCTTTCTTCAAGCCTGTAAGCGACGCCTCTCCGCCGTCTACCTCTAAGCGCAACACCAAAATCTCCGTCATCGGCGCTGGAAATGTCGGCATGGCCATTGCTCAGACCATCCTCACTCAAGATCTTGCCGATGAACTCGTCCTTGTCGACGCCAAGCCTGACAAACTCCGAGGCGAAATGCTCGATCTCCAACACGCCGCTGCTTTCCTTCCACGCACTAAAATCAGCGCCTCTACCGATTACTCTGTTACTGCCGAGTCCGATCTTTGCATCGTCACTGCTGGTGCTCGCCAGATCGCTGGTGAATCTAGGTTGAATCTGCTGCAGAGGAATGTTGCTCTGTTTCAGAAGATCGTGCCGCCGCTTGTTCAGTACTCTCCCGAGACGATTCTGCTGATCGTTTCGAATCCGGTGGATGTTTTGACTTATGTTGCTTGGAAGCTCTCTGGTTTTCCGTCGAATCGAGTGATTGGATCGGGAACGAATCTGGATTCGTCTAGGTTTCGGTTTCTCATCGCTGATCATCTCGACGTTAACGCTCAGGATGTTCAGGTCAGTTTTCCGATCGACATTTCTTCTACCTTCTTTAATCGATTAGTGCTTCTTGTTAATTTGCTGTTGCTGAAAAACTTTCAACTTAGGGTTTGGAACTTTTCGATTTTATCGTTTTTTATTGATTGATATCAAATGTGGCTTAGAATAAGCTTCCCTGAAATTTGGAAGATAATTTCCTAATTTCTGGCAACTAAACCACAATAATTAACAATATACTATACTTTAAATAATATTTAATGGATATTTTCTTTGAATTTTGTTAGATATGGAAAGTTTAGAGATCCATTTGGCACTTTTTAAAGTTAGATGGTTATAGGTGTTTTTTTTGTCTCATTTTGGTCTTACTATATCCTCTAGTGAGCATAGTAAGAGATAAAAACGAGAAAAAAGTATGTTTGAACGTCTTAATGGGAGCTCGTCTGAAAAATGAAGAATGTCGTCAATAGTACTCCTACAAAAAATGGCTCGGGACTGTTATCTATTGAAAATGTAGGGTCAATTTCATGGAATACGAACATTCTGCATAAAACAATTGGTCTTAACTCAGTTTTTTCTCATAATGTTTCAAACGCCTTAAATAGAGTGCTCATGTGTAATTTTAGATGGTAAGGTCTCGTGAACATAGCTATATTTGTTTGAAGGCATACATAGTGGGAGAGCACGGCGACAGCTCGGTGGCGCTATGGTCAAGCATAAGCGTGGGCGGAGTGCCAATACTGAGCTTTCTACAGAATCAACAAATCACCTACGAGAAGGAAACATTAGAGAGCATTCACAAAGAGGTCATAGGCGGGGCTTATGAAGTGATAAACTTGAAGGGCTACACATCGTGGGCGATCGGCTACTCGGTGGCAAGCCTCGCCCGGTCGCTCCTCCGTGACCAGCGTAGGATCCACCCTGTCTCTGTCCTTGCTAAAGGGTTCTACGGCATCAATGGAGGTGAGCTGTTCCTCAGCCTGCCCGCGCAGCTTGGTAGGGGCGGAGTATTGGGGATCACCAATGTGCATCTAACCGACGAGGAGTCGGGACGGCTGAGGGACTCAGCCAACACCATCTTGCAGGTGCAGACCCAGTTGGGGATCTGA

mRNA sequence

ATGGAGAAGAGTTCATCAGCATCTTCCTTAGGTCCAGGCGGCCTTGACATTTCGCAGGCTTTCTTCAAGCCTGTAAGCGACGCCTCTCCGCCGTCTACCTCTAAGCGCAACACCAAAATCTCCGTCATCGGCGCTGGAAATGTCGGCATGGCCATTGCTCAGACCATCCTCACTCAAGATCTTGCCGATGAACTCGTCCTTGTCGACGCCAAGCCTGACAAACTCCGAGGCGAAATGCTCGATCTCCAACACGCCGCTGCTTTCCTTCCACGCACTAAAATCAGCGCCTCTACCGATTACTCTGTTACTGCCGAGTCCGATCTTTGCATCGTCACTGCTGGTGCTCGCCAGATCGCTGGTGAATCTAGGTTGAATCTGCTGCAGAGGAATGTTGCTCTGTTTCAGAAGATCGTGCCGCCGCTTGTTCAGTACTCTCCCGAGACGATTCTGCTGATCGTTTCGAATCCGGTGGATGTTTTGACTTATGTTGCTTGGAAGCTCTCTGGTTTTCCGTCGAATCGAGTGATTGGATCGGGAACGAATCTGGATTCGTCTAGGTTTCGGTTTCTCATCGCTGATCATCTCGACGTTAACGCTCAGGATGTTCAGGCATACATAGTGGGAGAGCACGGCGACAGCTCGGTGGCGCTATGGTCAAGCATAAGCGTGGGCGGAGTGCCAATACTGAGCTTTCTACAGAATCAACAAATCACCTACGAGAAGGAAACATTAGAGAGCATTCACAAAGAGGTCATAGGCGGGGCTTATGAAGTGATAAACTTGAAGGGCTACACATCGTGGGCGATCGGCTACTCGGTGGCAAGCCTCGCCCGGTCGCTCCTCCGTGACCAGCGTAGGATCCACCCTGTCTCTGTCCTTGCTAAAGGGTTCTACGGCATCAATGGAGGTGAGCTGTTCCTCAGCCTGCCCGCGCAGCTTGGTAGGGGCGGAGTATTGGGGATCACCAATGTGCATCTAACCGACGAGGAGTCGGGACGGCTGAGGGACTCAGCCAACACCATCTTGCAGGTGCAGACCCAGTTGGGGATCTGA

Coding sequence (CDS)

ATGGAGAAGAGTTCATCAGCATCTTCCTTAGGTCCAGGCGGCCTTGACATTTCGCAGGCTTTCTTCAAGCCTGTAAGCGACGCCTCTCCGCCGTCTACCTCTAAGCGCAACACCAAAATCTCCGTCATCGGCGCTGGAAATGTCGGCATGGCCATTGCTCAGACCATCCTCACTCAAGATCTTGCCGATGAACTCGTCCTTGTCGACGCCAAGCCTGACAAACTCCGAGGCGAAATGCTCGATCTCCAACACGCCGCTGCTTTCCTTCCACGCACTAAAATCAGCGCCTCTACCGATTACTCTGTTACTGCCGAGTCCGATCTTTGCATCGTCACTGCTGGTGCTCGCCAGATCGCTGGTGAATCTAGGTTGAATCTGCTGCAGAGGAATGTTGCTCTGTTTCAGAAGATCGTGCCGCCGCTTGTTCAGTACTCTCCCGAGACGATTCTGCTGATCGTTTCGAATCCGGTGGATGTTTTGACTTATGTTGCTTGGAAGCTCTCTGGTTTTCCGTCGAATCGAGTGATTGGATCGGGAACGAATCTGGATTCGTCTAGGTTTCGGTTTCTCATCGCTGATCATCTCGACGTTAACGCTCAGGATGTTCAGGCATACATAGTGGGAGAGCACGGCGACAGCTCGGTGGCGCTATGGTCAAGCATAAGCGTGGGCGGAGTGCCAATACTGAGCTTTCTACAGAATCAACAAATCACCTACGAGAAGGAAACATTAGAGAGCATTCACAAAGAGGTCATAGGCGGGGCTTATGAAGTGATAAACTTGAAGGGCTACACATCGTGGGCGATCGGCTACTCGGTGGCAAGCCTCGCCCGGTCGCTCCTCCGTGACCAGCGTAGGATCCACCCTGTCTCTGTCCTTGCTAAAGGGTTCTACGGCATCAATGGAGGTGAGCTGTTCCTCAGCCTGCCCGCGCAGCTTGGTAGGGGCGGAGTATTGGGGATCACCAATGTGCATCTAACCGACGAGGAGTCGGGACGGCTGAGGGACTCAGCCAACACCATCTTGCAGGTGCAGACCCAGTTGGGGATCTGA

Protein sequence

MEKSSSASSLGPGGLDISQAFFKPVSDASPPSTSKRNTKISVIGAGNVGMAIAQTILTQDLADELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSVTAESDLCIVTAGARQIAGESRLNLLQRNVALFQKIVPPLVQYSPETILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGTNLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLQNQQITYEKETLESIHKEVIGGAYEVINLKGYTSWAIGYSVASLARSLLRDQRRIHPVSVLAKGFYGINGGELFLSLPAQLGRGGVLGITNVHLTDEESGRLRDSANTILQVQTQLGI

Homology

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BLAST of Carg23210-RA vs. NCBI nr
Match: XP_022922404.1 (L-lactate dehydrogenase A-like isoform X1 [Cucurbita moschata] >XP_022922405.1 L-lactate dehydrogenase A-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 673.3 bits (1736), Expect = 4.4e-190
Identity = 350/350 (100.00%), Postives = 350/350 (100.00%), Query Frame = 0


Query: 1   MEKSSSASSLGPGGLDISQAFFKPVSDASPPSTSKRNTKISVIGAGNVGMAIAQTILTQD 60
           MEKSSSASSLGPGGLDISQAFFKPVSDASPPSTSKRNTKISVIGAGNVGMAIAQTILTQD
Sbjct: 1   MEKSSSASSLGPGGLDISQAFFKPVSDASPPSTSKRNTKISVIGAGNVGMAIAQTILTQD 60

Query: 61  LADELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSVTAESDLCIVTAGARQIAG 120
           LADELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSVTAESDLCIVTAGARQIAG
Sbjct: 61  LADELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSVTAESDLCIVTAGARQIAG 120

Query: 121 ESRLNLLQRNVALFQKIVPPLVQYSPETILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT 180
           ESRLNLLQRNVALFQKIVPPLVQYSPETILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT
Sbjct: 121 ESRLNLLQRNVALFQKIVPPLVQYSPETILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT 180

Query: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLQNQQITYE 240
           NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLQNQQITYE
Sbjct: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLQNQQITYE 240

Query: 241 KETLESIHKEVIGGAYEVINLKGYTSWAIGYSVASLARSLLRDQRRIHPVSVLAKGFYGI 300
           KETLESIHKEVIGGAYEVINLKGYTSWAIGYSVASLARSLLRDQRRIHPVSVLAKGFYGI
Sbjct: 241 KETLESIHKEVIGGAYEVINLKGYTSWAIGYSVASLARSLLRDQRRIHPVSVLAKGFYGI 300

Query: 301 NGGELFLSLPAQLGRGGVLGITNVHLTDEESGRLRDSANTILQVQTQLGI 351
           NGGELFLSLPAQLGRGGVLGITNVHLTDEESGRLRDSANTILQVQTQLGI
Sbjct: 301 NGGELFLSLPAQLGRGGVLGITNVHLTDEESGRLRDSANTILQVQTQLGI 350

[Close]

BLAST of Carg23210-RA vs. NCBI nr
Match: XP_022969721.1 (L-lactate dehydrogenase A-like [Cucurbita maxima])

HSP 1 Score: 670.6 bits (1729), Expect = 2.8e-189
Identity = 348/350 (99.43%), Postives = 350/350 (100.00%), Query Frame = 0


Query: 1   MEKSSSASSLGPGGLDISQAFFKPVSDASPPSTSKRNTKISVIGAGNVGMAIAQTILTQD 60
           MEKSSSASSLGPGGL+ISQAFFKPVSDASPPSTSKRNTKISVIGAGNVGMAIAQTILTQD
Sbjct: 1   MEKSSSASSLGPGGLNISQAFFKPVSDASPPSTSKRNTKISVIGAGNVGMAIAQTILTQD 60

Query: 61  LADELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSVTAESDLCIVTAGARQIAG 120
           LADELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSVTAESDLCIVTAGARQIAG
Sbjct: 61  LADELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSVTAESDLCIVTAGARQIAG 120

Query: 121 ESRLNLLQRNVALFQKIVPPLVQYSPETILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT 180
           ESRLNLLQRNVALFQKIVPPLVQYSPETILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT
Sbjct: 121 ESRLNLLQRNVALFQKIVPPLVQYSPETILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT 180

Query: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLQNQQITYE 240
           NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLQNQQITYE
Sbjct: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLQNQQITYE 240

Query: 241 KETLESIHKEVIGGAYEVINLKGYTSWAIGYSVASLARSLLRDQRRIHPVSVLAKGFYGI 300
           KETLESIHKEVIGGAYEVINLKGYTSWAIGYSVASLARSLLRDQRRIHPVSVLAKGFYGI
Sbjct: 241 KETLESIHKEVIGGAYEVINLKGYTSWAIGYSVASLARSLLRDQRRIHPVSVLAKGFYGI 300

Query: 301 NGGELFLSLPAQLGRGGVLGITNVHLTDEESGRLRDSANTILQVQTQLGI 351
           NGGE+FLSLPAQLGRGGVLGITNVHLTDEESGRLRDSANTILQVQTQLGI
Sbjct: 301 NGGEMFLSLPAQLGRGGVLGITNVHLTDEESGRLRDSANTILQVQTQLGI 350

[Close]

BLAST of Carg23210-RA vs. NCBI nr
Match: XP_023549806.1 (L-lactate dehydrogenase A-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 670.6 bits (1729), Expect = 2.8e-189
Identity = 349/350 (99.71%), Postives = 349/350 (99.71%), Query Frame = 0


Query: 1   MEKSSSASSLGPGGLDISQAFFKPVSDASPPSTSKRNTKISVIGAGNVGMAIAQTILTQD 60
           MEKSSSASSLGPGGLDISQAFFKPVSDASPPSTSKRNTKISVIGAGNVGMAIAQTILTQD
Sbjct: 1   MEKSSSASSLGPGGLDISQAFFKPVSDASPPSTSKRNTKISVIGAGNVGMAIAQTILTQD 60

Query: 61  LADELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSVTAESDLCIVTAGARQIAG 120
           LADELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSVTAESDLCIVTAGARQIAG
Sbjct: 61  LADELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSVTAESDLCIVTAGARQIAG 120

Query: 121 ESRLNLLQRNVALFQKIVPPLVQYSPETILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT 180
           ESRLNLLQRNVALFQKIVPPLVQYSP TILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT
Sbjct: 121 ESRLNLLQRNVALFQKIVPPLVQYSPGTILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT 180

Query: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLQNQQITYE 240
           NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLQNQQITYE
Sbjct: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLQNQQITYE 240

Query: 241 KETLESIHKEVIGGAYEVINLKGYTSWAIGYSVASLARSLLRDQRRIHPVSVLAKGFYGI 300
           KETLESIHKEVIGGAYEVINLKGYTSWAIGYSVASLARSLLRDQRRIHPVSVLAKGFYGI
Sbjct: 241 KETLESIHKEVIGGAYEVINLKGYTSWAIGYSVASLARSLLRDQRRIHPVSVLAKGFYGI 300

Query: 301 NGGELFLSLPAQLGRGGVLGITNVHLTDEESGRLRDSANTILQVQTQLGI 351
           NGGELFLSLPAQLGRGGVLGITNVHLTDEESGRLRDSANTILQVQTQLGI
Sbjct: 301 NGGELFLSLPAQLGRGGVLGITNVHLTDEESGRLRDSANTILQVQTQLGI 350

[Close]

BLAST of Carg23210-RA vs. NCBI nr
Match: XP_008437100.2 (PREDICTED: L-lactate dehydrogenase A [Cucumis melo])

HSP 1 Score: 638.3 bits (1645), Expect = 1.6e-179
Identity = 329/350 (94.00%), Postives = 339/350 (96.86%), Query Frame = 0


Query: 1   MEKSSSASSLGPGGLDISQAFFKPVSDASPPSTSKRNTKISVIGAGNVGMAIAQTILTQD 60
           M+KS S SSLGPGGLD+SQAFFKP+ DASPPSTS RNTKISVIGAGNVGMAIAQTILTQD
Sbjct: 1   MQKSPSTSSLGPGGLDLSQAFFKPIRDASPPSTSNRNTKISVIGAGNVGMAIAQTILTQD 60

Query: 61  LADELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSVTAESDLCIVTAGARQIAG 120
           L DELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYS TA S+LCIVTAGARQIAG
Sbjct: 61  LVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSTTAGSNLCIVTAGARQIAG 120

Query: 121 ESRLNLLQRNVALFQKIVPPLVQYSPETILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT 180
           ESRLNLLQRNVALFQKIVPPLVQ+SPETILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT
Sbjct: 121 ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT 180

Query: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLQNQQITYE 240
           NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFL+NQQI YE
Sbjct: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIAYE 240

Query: 241 KETLESIHKEVIGGAYEVINLKGYTSWAIGYSVASLARSLLRDQRRIHPVSVLAKGFYGI 300
           KETLE IHKEVIGGAYEVINLKGYTSWAIGYSVASLARS+LRDQRRIHPVSVLAKGFYGI
Sbjct: 241 KETLEKIHKEVIGGAYEVINLKGYTSWAIGYSVASLARSILRDQRRIHPVSVLAKGFYGI 300

Query: 301 NGGELFLSLPAQLGRGGVLGITNVHLTDEESGRLRDSANTILQVQTQLGI 351
           +GG+LFLSLPAQLGRGGVLGITNVHLT EES RLRDSANTILQVQT+LGI
Sbjct: 301 DGGDLFLSLPAQLGRGGVLGITNVHLTAEESKRLRDSANTILQVQTELGI 350

[Close]

BLAST of Carg23210-RA vs. NCBI nr
Match: XP_004151663.2 (PREDICTED: LOW QUALITY PROTEIN: L-lactate dehydrogenase A-like [Cucumis sativus])

HSP 1 Score: 634.4 bits (1635), Expect = 2.3e-178
Identity = 326/350 (93.14%), Postives = 339/350 (96.86%), Query Frame = 0


Query: 1   MEKSSSASSLGPGGLDISQAFFKPVSDASPPSTSKRNTKISVIGAGNVGMAIAQTILTQD 60
           M+KSSS SSLGPGGLD+SQAFFKP+ DASPPST+ RNTKISVIGAGNVGMAIAQTILTQD
Sbjct: 1   MQKSSSISSLGPGGLDLSQAFFKPIRDASPPSTANRNTKISVIGAGNVGMAIAQTILTQD 60

Query: 61  LADELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSVTAESDLCIVTAGARQIAG 120
           L DELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYS+TA SDLCIVT GARQIAG
Sbjct: 61  LVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTGGARQIAG 120

Query: 121 ESRLNLLQRNVALFQKIVPPLVQYSPETILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT 180
           ESRLNLLQRNVALFQKIVPPLVQ+SPETILLIVSNPVDVLTY+AWKLSGFPSNRVI SGT
Sbjct: 121 ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYIAWKLSGFPSNRVIXSGT 180

Query: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLQNQQITYE 240
           NL+SSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFL+NQQI YE
Sbjct: 181 NLNSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIGYE 240

Query: 241 KETLESIHKEVIGGAYEVINLKGYTSWAIGYSVASLARSLLRDQRRIHPVSVLAKGFYGI 300
           KETLE IHKEVIGGAYEVINLKGYTSWAIGYSVASLARS+LRDQRRIHPVSVLAKGFYGI
Sbjct: 241 KETLEKIHKEVIGGAYEVINLKGYTSWAIGYSVASLARSILRDQRRIHPVSVLAKGFYGI 300

Query: 301 NGGELFLSLPAQLGRGGVLGITNVHLTDEESGRLRDSANTILQVQTQLGI 351
           +GG+LFLSLPAQLGRGGVLGITNVHLT EES RLRDSANTILQVQT+LGI
Sbjct: 301 DGGDLFLSLPAQLGRGGVLGITNVHLTAEESKRLRDSANTILQVQTELGI 350

[Close]

BLAST of Carg23210-RA vs. TAIR10
Match: AT4G17260.1 (Lactate/malate dehydrogenase family protein)

HSP 1 Score: 546.6 bits (1407), Expect = 1.1e-155
Identity = 271/353 (76.77%), Postives = 316/353 (89.52%), Query Frame = 0


Query: 1   MEKSSSASS---LGPGGLDISQAFFKPVSDASPPSTSKRNTKISVIGAGNVGMAIAQTIL 60
           MEK++S SS   LGP GLD++ AFFKP+ ++ P   S R TK+SV+G GNVGMAIAQTIL
Sbjct: 1   MEKNASTSSLKDLGPSGLDLTSAFFKPIHNSDPSLPSNRRTKVSVVGVGNVGMAIAQTIL 60

Query: 61  TQDLADELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSVTAESDLCIVTAGARQ 120
           TQDLADE+ LVDAKPDKLRGEMLDLQHAAAFLPRTKI+AS DY VTA SDLCIVTAGARQ
Sbjct: 61  TQDLADEIALVDAKPDKLRGEMLDLQHAAAFLPRTKITASVDYEVTAGSDLCIVTAGARQ 120

Query: 121 IAGESRLNLLQRNVALFQKIVPPLVQYSPETILLIVSNPVDVLTYVAWKLSGFPSNRVIG 180
             GESRLNLLQRNVALF+ I+PPL + SP++IL+IVSNPVDVLTYVAWKLSGFP NRV+G
Sbjct: 121 NPGESRLNLLQRNVALFRHIIPPLAKASPDSILIIVSNPVDVLTYVAWKLSGFPVNRVLG 180

Query: 181 SGTNLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLQNQQI 240
           SGTNLDSSRFRFLIADHLDVNAQDVQA+IVGEHGDSSVALWSSISVGG+P+LSFL+  QI
Sbjct: 181 SGTNLDSSRFRFLIADHLDVNAQDVQAFIVGEHGDSSVALWSSISVGGIPVLSFLEKNQI 240

Query: 241 TYEKETLESIHKEVIGGAYEVINLKGYTSWAIGYSVASLARSLLRDQRRIHPVSVLAKGF 300
            YEK+TLE IH+ V+G AYEVI LKGYTSWAIGYSVA+LAR++LRDQR+IHPV+VLA+GF
Sbjct: 241 AYEKQTLEDIHQAVVGSAYEVIGLKGYTSWAIGYSVANLARTILRDQRKIHPVTVLARGF 300

Query: 301 YGINGGELFLSLPAQLGRGGVLGITNVHLTDEESGRLRDSANTILQVQTQLGI 351
           YG++GG++FLSLPA LGR GV+ +TNVH+TDEE+ +L+ SA TIL++Q+QLG+
Sbjct: 301 YGVDGGDVFLSLPALLGRNGVVAVTNVHMTDEEAEKLQKSAKTILEMQSQLGL 353

[Close]

BLAST of Carg23210-RA vs. Swiss-Prot
Match: sp|P22988|LDHA_HORVU (L-lactate dehydrogenase A OS=Hordeum vulgare OX=4513 PE=1 SV=1)

HSP 1 Score: 468.8 bits (1205), Expect = 5.4e-131
Identity = 246/348 (70.69%), Postives = 291/348 (83.62%), Query Frame = 0


Query: 1   MEKSSSASSLGPGGLDISQAFFKPVSDASP--PSTS----KRNTKISVIGAGNVGMAIAQ 60
           M K+SS S LG      S  FF+PV+D  P  P++S    +R TKISVIGAGNVGMAIAQ
Sbjct: 1   MHKASSLSELGFDAGGASSGFFRPVADGCPATPTSSAVPHRRLTKISVIGAGNVGMAIAQ 60

Query: 61  TILTQDLADELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSVTAESDLCIVTAG 120
           TILTQ+LADE+ LVDA PDKLRGE LDLQHAAAFLPR +IS  TD +VT  SDL IVTAG
Sbjct: 61  TILTQNLADEIALVDALPDKLRGEALDLQHAAAFLPRVRIS-GTDAAVTKNSDLVIVTAG 120

Query: 121 ARQIAGESRLNLLQRNVALFQKIVPPLVQYSPETILLIVSNPVDVLTYVAWKLSGFPSNR 180
           ARQI GE+RLNLLQRNVAL++KIVPP+ ++SP+ +LL+VSNPVDVLTYVAWKLSGFP++R
Sbjct: 121 ARQIPGETRLNLLQRNVALYRKIVPPVAEHSPDALLLVVSNPVDVLTYVAWKLSGFPASR 180

Query: 181 VIGSGTNLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLQN 240
           VIGSGTNLDSSRFRFLIA+HLDVNAQDVQAY+VGEHGDSSVA+WSSISVGG+P    L++
Sbjct: 181 VIGSGTNLDSSRFRFLIAEHLDVNAQDVQAYMVGEHGDSSVAIWSSISVGGMPAFKSLRD 240

Query: 241 QQITYEKETLESIHKEVIGGAYEVINLKGYTSWAIGYSVASLARSLLRDQRRIHPVSVLA 300
              ++++  LE I + V+GGAYEVI LKGYTSWAIGYSVASLA SLLRDQRR+HPVSVLA
Sbjct: 241 SHRSFDEAALEGIRRAVVGGAYEVIGLKGYTSWAIGYSVASLAASLLRDQRRVHPVSVLA 300

Query: 301 KGFYGINGG-ELFLSLPAQLGRGGVLGITNVHLTDEESGRLRDSANTI 342
            GF+GI+ G E+FLSLPA+LGRGG+LG+  + LT+ E+ +LR SA T+
Sbjct: 301 SGFHGISDGHEVFLSLPARLGRGGILGVAEMDLTEAEAAQLRRSAKTL 347

[Close]

BLAST of Carg23210-RA vs. Swiss-Prot
Match: sp|P22989|LDHB_HORVU (L-lactate dehydrogenase B (Fragment) OS=Hordeum vulgare OX=4513 PE=1 SV=1)

HSP 1 Score: 459.1 bits (1180), Expect = 4.3e-128
Identity = 235/342 (68.71%), Postives = 284/342 (83.04%), Query Frame = 0


Query: 16  DISQAFFKPVSDASP------PSTSKRNTKISVIGAGNVGMAIAQTILTQDLADELVLVD 75
           D S  FF+ V+D  P       +  +R TK+SVIGAGNVGMAIAQTILTQ+LADE+ LVD
Sbjct: 3   DASSGFFRAVADGCPITHTSCSAPHRRLTKVSVIGAGNVGMAIAQTILTQNLADEIALVD 62

Query: 76  AKPDKLRGEMLDLQHAAAFLPRTKISASTDYSVTAESDLCIVTAGARQIAGESRLNLLQR 135
           A PDKLRGE LDLQHAAAFLPR +I + TD +VT  SDL +VTAGARQI GE+RLNLLQR
Sbjct: 63  ALPDKLRGEALDLQHAAAFLPRVRIVSGTDAAVTKNSDLIVVTAGARQIPGETRLNLLQR 122

Query: 136 NVALFQKIVPPLVQYSPETILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGTNLDSSRFRF 195
           NVAL++KIVPP+ ++SP+ +LL+VSNPVDVLTYVAWKLSGFP++RVIGSGTNLDSSRFRF
Sbjct: 123 NVALYRKIVPPVAEHSPDALLLVVSNPVDVLTYVAWKLSGFPASRVIGSGTNLDSSRFRF 182

Query: 196 LIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLQNQQITYEKETLESIHK 255
           L+A+HLDV+AQDVQAY+VGEHGDSSVA+WSSISVGG+P L  L++   ++++  LE I +
Sbjct: 183 LVAEHLDVSAQDVQAYMVGEHGDSSVAIWSSISVGGMPALKSLRDSHRSFDEAALEGIRR 242

Query: 256 EVIGGAYEVINLKGYTSWAIGYSVASLARSLLRDQRRIHPVSVLAKGFYGINGG-ELFLS 315
            V+GGAYEVI LKGYTSWAIGYSVASLA SLLRDQRR+HPVSVLA GF+GI+ G E+FLS
Sbjct: 243 AVVGGAYEVIGLKGYTSWAIGYSVASLATSLLRDQRRVHPVSVLAAGFHGISDGHEVFLS 302

Query: 316 LPAQLGRGGVLGITNVHLTDEESGRLRDSANTILQVQTQLGI 351
           LPA+LGR GVLG+  + LT+ E+ +LR SA T+ +    LG+
Sbjct: 303 LPARLGRAGVLGVAEMDLTEAEAAQLRRSAKTLWENCQLLGL 344

[Close]

BLAST of Carg23210-RA vs. Swiss-Prot
Match: sp|P29038|LDH_MAIZE (L-lactate dehydrogenase OS=Zea mays OX=4577 PE=2 SV=1)

HSP 1 Score: 441.0 bits (1133), Expect = 1.2e-122
Identity = 237/354 (66.95%), Postives = 280/354 (79.10%), Query Frame = 0


Query: 1   MEKSSSASSLGPGGLDISQAFFKPVS-DASPPSTS---KRNTKISVIGAGNVGMAIAQTI 60
           M+K++S S LG    D S  FF+PVS D+S P++    +R TK+SVIGAGNVGMAIAQTI
Sbjct: 1   MKKATSLSELGFDAGDASSGFFRPVSGDSSTPTSQHHRRRLTKVSVIGAGNVGMAIAQTI 60

Query: 61  LTQDLADELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSVTAESDLCIVTAGAR 120
           LT+DLADE+ LVDA PDKLRGEMLDLQHAAAFLPRT++ + TD SVT  SDL IVTAGAR
Sbjct: 61  LTRDLADEIALVDAVPDKLRGEMLDLQHAAAFLPRTRLVSGTDMSVTRGSDLVIVTAGAR 120

Query: 121 QIAGESRLNLLQRNVALFQKIVPPLVQYSPETILLIVSNPVDVLTYVAWKLSGFPSNRVI 180
           QI GE+RL+LLQRNVALF+KIVPPL + S + +LL+VSNPVDVLTYVAWKLSGFP++RVI
Sbjct: 121 QIQGETRLDLLQRNVALFRKIVPPLAEQSHDALLLVVSNPVDVLTYVAWKLSGFPASRVI 180

Query: 181 GSGTNLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLQNQQ 240
           GSGTNLDSSRFRFL+A+HLDVNAQDVQAY+VGEHGDSSVA+WSS+SV G+P+L  LQ   
Sbjct: 181 GSGTNLDSSRFRFLLAEHLDVNAQDVQAYMVGEHGDSSVAVWSSVSVAGMPVLKSLQESH 240

Query: 241 ITYEKETLESIHKEVIGGAYEVINLKGYTSWAIGYSVASLARSLLRDQRRIHPVSVLAKG 300
             +++E LE I + V+  AYEVI+LKGYTSWAIGYSVASLA SLLRDQRRIHPVSVLA+G
Sbjct: 241 RCFDEEALEGIRRAVVDSAYEVISLKGYTSWAIGYSVASLAASLLRDQRRIHPVSVLARG 300

Query: 301 FYGINGGELFLSLPAQLGRGGVLGITNVHLTDEESGRLRDSANTILQVQTQLGI 351
           F+GI  G    S               + LT+EE+ RLR SA TI +    LG+
Sbjct: 301 FHGIPDGTTSSSACPPRRXXXXXXRREMELTEEEAKRLRRSAKTIWENCQLLGL 354

[Close]

BLAST of Carg23210-RA vs. Swiss-Prot
Match: sp|P06151|LDHA_MOUSE (L-lactate dehydrogenase A chain OS=Mus musculus OX=10090 GN=Ldha PE=1 SV=3)

HSP 1 Score: 349.0 bits (894), Expect = 6.2e-95
Identity = 176/310 (56.77%), Postives = 228/310 (73.55%), Query Frame = 0


Query: 39  KISVIGAGNVGMAIAQTILTQDLADELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISAST 98
           KI+V+G G VGMA A +IL +DLADEL LVD   DKL+GEM+DLQH + FL   KI +S 
Sbjct: 22  KITVVGVGAVGMACAISILMKDLADELALVDVMEDKLKGEMMDLQHGSLFLKTPKIVSSK 81

Query: 99  DYSVTAESDLCIVTAGARQIAGESRLNLLQRNVALFQKIVPPLVQYSPETILLIVSNPVD 158
           DY VTA S L I+TAGARQ  GESRLNL+QRNV +F+ I+P +V+YSP   LLIVSNPVD
Sbjct: 82  DYCVTANSKLVIITAGARQQEGESRLNLVQRNVNIFKFIIPNIVKYSPHCKLLIVSNPVD 141

Query: 159 VLTYVAWKLSGFPSNRVIGSGTNLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALW 218
           +LTYVAWK+SGFP NRVIGSG NLDS+RFR+L+ + L V+A     +++GEHGDSSV +W
Sbjct: 142 ILTYVAWKISGFPKNRVIGSGCNLDSARFRYLMGERLGVHALSCHGWVLGEHGDSSVPVW 201

Query: 219 SSISVGGVPILSFLQNQQITYEKETLESIHKEVIGGAYEVINLKGYTSWAIGYSVASLAR 278
           S ++V GV + S         +KE  + +HK+V+  AYEVI LKGYTSWAIG SVA LA 
Sbjct: 202 SGVNVAGVSLKSLNPELGTDADKEQWKEVHKQVVDSAYEVIKLKGYTSWAIGLSVADLAE 261

Query: 279 SLLRDQRRIHPVSVLAKGFYGINGGELFLSLPAQLGRGGVLGITNVHLTDEESGRLRDSA 338
           S++++ RR+HP+S + KG YGIN  ++FLS+P  LG+ G+  +  V LT EE  RL+ SA
Sbjct: 262 SIMKNLRRVHPISTMIKGLYGIN-EDVFLSVPCILGQNGISDVVKVTLTPEEEARLKKSA 321

Query: 339 NTILQVQTQL 349
           +T+  +Q +L
Sbjct: 322 DTLWGIQKEL 330

[Close]

BLAST of Carg23210-RA vs. Swiss-Prot
Match: sp|Q9XT87|LDHA_MONDO (L-lactate dehydrogenase A chain OS=Monodelphis domestica OX=13616 GN=LDHA PE=2 SV=3)

HSP 1 Score: 345.9 bits (886), Expect = 5.3e-94
Identity = 173/312 (55.45%), Postives = 229/312 (73.40%), Query Frame = 0


Query: 37  NTKISVIGAGNVGMAIAQTILTQDLADELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISA 96
           + KI+V+G G VGMA A +IL +DLADEL LVD   DKL+GEM+DLQH + FL   KI +
Sbjct: 20  HNKITVVGVGAVGMACAISILMKDLADELALVDVIEDKLKGEMMDLQHGSLFLKTPKIVS 79

Query: 97  STDYSVTAESDLCIVTAGARQIAGESRLNLLQRNVALFQKIVPPLVQYSPETILLIVSNP 156
           S DY+VTA S L ++TAGARQ  GESRLNL+QRNV +F+ I+P +V+YSP   LL+VSNP
Sbjct: 80  SKDYAVTANSKLVVITAGARQQEGESRLNLVQRNVNIFKFIIPNIVKYSPNCKLLVVSNP 139

Query: 157 VDVLTYVAWKLSGFPSNRVIGSGTNLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVA 216
           VD+LTYVAWKLSGFP NRVIGSG NLDS+RFR+L+ + L +++     +I+GEHGDSSV 
Sbjct: 140 VDILTYVAWKLSGFPKNRVIGSGCNLDSARFRYLMGEKLGIHSSSCHGWILGEHGDSSVP 199

Query: 217 LWSSISVGGVPILSFLQNQQITYEKETLESIHKEVIGGAYEVINLKGYTSWAIGYSVASL 276
           +WS ++V GV + S         + E  + +HK+V+  AYEVI LKGYTSWAIG SVA L
Sbjct: 200 VWSGVNVAGVSLKSLHPALGTDSDSEQWKDVHKQVVESAYEVIKLKGYTSWAIGLSVADL 259

Query: 277 ARSLLRDQRRIHPVSVLAKGFYGINGGELFLSLPAQLGRGGVLGITNVHLTDEESGRLRD 336
           A S++++ RR+HP+S + KG YGIN  ++FLS+P  LG+ G+  +  V LT EE  RL+ 
Sbjct: 260 AESIMKNLRRVHPISTMIKGLYGIN-EDVFLSVPCILGQNGISDVVKVTLTTEEESRLKQ 319

Query: 337 SANTILQVQTQL 349
           SA+T+  +Q +L
Sbjct: 320 SADTLWGIQKEL 330

[Close]

BLAST of Carg23210-RA vs. TrEMBL
Match: tr|A0A1S3ATB9|A0A1S3ATB9_CUCME (L-lactate dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103482619 PE=3 SV=1)

HSP 1 Score: 638.3 bits (1645), Expect = 1.0e-179
Identity = 329/350 (94.00%), Postives = 339/350 (96.86%), Query Frame = 0


Query: 1   MEKSSSASSLGPGGLDISQAFFKPVSDASPPSTSKRNTKISVIGAGNVGMAIAQTILTQD 60
           M+KS S SSLGPGGLD+SQAFFKP+ DASPPSTS RNTKISVIGAGNVGMAIAQTILTQD
Sbjct: 1   MQKSPSTSSLGPGGLDLSQAFFKPIRDASPPSTSNRNTKISVIGAGNVGMAIAQTILTQD 60

Query: 61  LADELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSVTAESDLCIVTAGARQIAG 120
           L DELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYS TA S+LCIVTAGARQIAG
Sbjct: 61  LVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSTTAGSNLCIVTAGARQIAG 120

Query: 121 ESRLNLLQRNVALFQKIVPPLVQYSPETILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT 180
           ESRLNLLQRNVALFQKIVPPLVQ+SPETILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT
Sbjct: 121 ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT 180

Query: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLQNQQITYE 240
           NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFL+NQQI YE
Sbjct: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIAYE 240

Query: 241 KETLESIHKEVIGGAYEVINLKGYTSWAIGYSVASLARSLLRDQRRIHPVSVLAKGFYGI 300
           KETLE IHKEVIGGAYEVINLKGYTSWAIGYSVASLARS+LRDQRRIHPVSVLAKGFYGI
Sbjct: 241 KETLEKIHKEVIGGAYEVINLKGYTSWAIGYSVASLARSILRDQRRIHPVSVLAKGFYGI 300

Query: 301 NGGELFLSLPAQLGRGGVLGITNVHLTDEESGRLRDSANTILQVQTQLGI 351
           +GG+LFLSLPAQLGRGGVLGITNVHLT EES RLRDSANTILQVQT+LGI
Sbjct: 301 DGGDLFLSLPAQLGRGGVLGITNVHLTAEESKRLRDSANTILQVQTELGI 350

[Close]

BLAST of Carg23210-RA vs. TrEMBL
Match: tr|A0A0A0KKU9|A0A0A0KKU9_CUCSA (L-lactate dehydrogenase OS=Cucumis sativus OX=3659 GN=Csa_5G161920 PE=3 SV=1)

HSP 1 Score: 618.2 bits (1593), Expect = 1.1e-173
Identity = 320/350 (91.43%), Postives = 331/350 (94.57%), Query Frame = 0


Query: 1   MEKSSSASSLGPGGLDISQAFFKPVSDASPPSTSKRNTKISVIGAGNVGMAIAQTILTQD 60
           M+KS S SSLGPGGLD+SQAFFKP+ DASP STS  NTKISVIG GNVGMAIAQTILTQD
Sbjct: 63  MQKSPSTSSLGPGGLDLSQAFFKPIRDASPLSTSNCNTKISVIGTGNVGMAIAQTILTQD 122

Query: 61  LADELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSVTAESDLCIVTAGARQIAG 120
           L DELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYS+TA SDLCIVTAGARQIAG
Sbjct: 123 LVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITARSDLCIVTAGARQIAG 182

Query: 121 ESRLNLLQRNVALFQKIVPPLVQYSPETILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT 180
           ESRLNLLQRNV LFQKIVP LVQ+SPETILLIVSNPVDVLTYVAWKLSGF SNRVIGSGT
Sbjct: 183 ESRLNLLQRNVTLFQKIVPLLVQFSPETILLIVSNPVDVLTYVAWKLSGFSSNRVIGSGT 242

Query: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLQNQQITYE 240
           NLDSSRFRFLIADHLDVNAQDVQA+IVGEHGDSSVALWSSISVGGVPILSFL+NQQI YE
Sbjct: 243 NLDSSRFRFLIADHLDVNAQDVQAFIVGEHGDSSVALWSSISVGGVPILSFLKNQQIGYE 302

Query: 241 KETLESIHKEVIGGAYEVINLKGYTSWAIGYSVASLARSLLRDQRRIHPVSVLAKGFYGI 300
           KETLE IHKEVIGGAYEVINLKGYTSWAIGYSVASLARS+LRDQRRIHPVSV+AKGFYGI
Sbjct: 303 KETLEKIHKEVIGGAYEVINLKGYTSWAIGYSVASLARSILRDQRRIHPVSVIAKGFYGI 362

Query: 301 NGGELFLSLPAQLGRGGVLGITNVHLTDEESGRLRDSANTILQVQTQLGI 351
            GG+LFLSLPAQLGRGGVLGITNVHLT EES RLRDSANTIL VQ +LGI
Sbjct: 363 EGGDLFLSLPAQLGRGGVLGITNVHLTTEESKRLRDSANTILHVQAELGI 412

[Close]

BLAST of Carg23210-RA vs. TrEMBL
Match: tr|A0A067KUG8|A0A067KUG8_JATCU (L-lactate dehydrogenase OS=Jatropha curcas OX=180498 GN=JCGZ_03382 PE=3 SV=1)

HSP 1 Score: 601.7 bits (1550), Expect = 1.1e-168
Identity = 303/350 (86.57%), Postives = 331/350 (94.57%), Query Frame = 0


Query: 1   MEKSSSASSLGPGGLDISQAFFKPVSDASPPSTSKRNTKISVIGAGNVGMAIAQTILTQD 60
           M+KS SAS+LGPGGLD++QAFFKP+ + +PPS +KR+TKISVIG GNVGMAIAQTILTQD
Sbjct: 1   MQKSPSASTLGPGGLDLTQAFFKPIQNTAPPSPTKRHTKISVIGTGNVGMAIAQTILTQD 60

Query: 61  LADELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSVTAESDLCIVTAGARQIAG 120
           LADEL LVDAKPDKLRGEMLDLQHAAAFLPRTKI ASTDYSVT  SDLCIVTAGARQI G
Sbjct: 61  LADELALVDAKPDKLRGEMLDLQHAAAFLPRTKIIASTDYSVTVGSDLCIVTAGARQIPG 120

Query: 121 ESRLNLLQRNVALFQKIVPPLVQYSPETILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT 180
           ESRLNLLQRNVALF+KI+PPLV YSPE+IL+IVSNPVDVLTY+AWKLSGFPSNRVIGSGT
Sbjct: 121 ESRLNLLQRNVALFEKIIPPLVNYSPESILMIVSNPVDVLTYIAWKLSGFPSNRVIGSGT 180

Query: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLQNQQITYE 240
           NLDSSRFRFLIADHLDVNAQDVQA+IVGEHGDSSVALWSSISVGGVPILSFL+ QQI YE
Sbjct: 181 NLDSSRFRFLIADHLDVNAQDVQAFIVGEHGDSSVALWSSISVGGVPILSFLEKQQIAYE 240

Query: 241 KETLESIHKEVIGGAYEVINLKGYTSWAIGYSVASLARSLLRDQRRIHPVSVLAKGFYGI 300
           KETLE+IHK VI  AYEVINLKGYTSWAIGYS A+LARSLLRDQR+IHPVSVLAKGFYGI
Sbjct: 241 KETLENIHKAVIDSAYEVINLKGYTSWAIGYSAANLARSLLRDQRKIHPVSVLAKGFYGI 300

Query: 301 NGGELFLSLPAQLGRGGVLGITNVHLTDEESGRLRDSANTILQVQTQLGI 351
           +GGE+FLSLPAQLGRGGVLG+TNVHLT+EE+ RLR+SA T+L+VQ+QLGI
Sbjct: 301 DGGEVFLSLPAQLGRGGVLGVTNVHLTEEEARRLRESAKTLLEVQSQLGI 350

[Close]

BLAST of Carg23210-RA vs. TrEMBL
Match: tr|A0A2P5F5D0|A0A2P5F5D0_9ROSA (L-lactate dehydrogenase OS=Trema orientalis OX=63057 GN=TorRG33x02_112750 PE=3 SV=1)

HSP 1 Score: 599.4 bits (1544), Expect = 5.3e-168
Identity = 301/350 (86.00%), Postives = 331/350 (94.57%), Query Frame = 0


Query: 1   MEKSSSASSLGPGGLDISQAFFKPVSDASPPSTSKRNTKISVIGAGNVGMAIAQTILTQD 60
           M+KS S SSLGPGGLD+S+AFFKP+  A+PPS +KR+TKISVIGAGNVGMAIAQTILTQD
Sbjct: 1   MQKSESTSSLGPGGLDLSEAFFKPIHRAAPPSPTKRHTKISVIGAGNVGMAIAQTILTQD 60

Query: 61  LADELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSVTAESDLCIVTAGARQIAG 120
           LADELVLVDAKPDKLRGEMLDLQHAAAFLPRTKI AS DY+VTA SDLCIVTAGARQI G
Sbjct: 61  LADELVLVDAKPDKLRGEMLDLQHAAAFLPRTKIHASVDYAVTAGSDLCIVTAGARQIPG 120

Query: 121 ESRLNLLQRNVALFQKIVPPLVQYSPETILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT 180
           ESRLNLLQRNVALF+ I+PPL +YSP+TILLIVSNPVDVLTYV+WKLSGFPSNRVIGSGT
Sbjct: 121 ESRLNLLQRNVALFRNIIPPLARYSPDTILLIVSNPVDVLTYVSWKLSGFPSNRVIGSGT 180

Query: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLQNQQITYE 240
           NLDSSRFRFLIADHLDVNAQDVQAYI+GEHGDSSVALWSSISVGGVP+LSFL+ QQI YE
Sbjct: 181 NLDSSRFRFLIADHLDVNAQDVQAYIIGEHGDSSVALWSSISVGGVPVLSFLERQQIPYE 240

Query: 241 KETLESIHKEVIGGAYEVINLKGYTSWAIGYSVASLARSLLRDQRRIHPVSVLAKGFYGI 300
           K+TLE+IHK+VIG AYEVI+LKGYTSWAIGYS A+LARS+LRDQR+IHPV+VLA G YGI
Sbjct: 241 KQTLENIHKQVIGSAYEVISLKGYTSWAIGYSAANLARSILRDQRKIHPVTVLATGLYGI 300

Query: 301 NGGELFLSLPAQLGRGGVLGITNVHLTDEESGRLRDSANTILQVQTQLGI 351
           +GGE+FLSLPAQLGRGGVLG+TNVHL+DEE  RLRDSANTIL+VQ+QLGI
Sbjct: 301 DGGEVFLSLPAQLGRGGVLGVTNVHLSDEEGQRLRDSANTILEVQSQLGI 350

[Close]

BLAST of Carg23210-RA vs. TrEMBL
Match: tr|A0A1R3GNR2|A0A1R3GNR2_COCAP (L-lactate dehydrogenase OS=Corchorus capsularis OX=210143 GN=CCACVL1_24669 PE=3 SV=1)

HSP 1 Score: 596.3 bits (1536), Expect = 4.5e-167
Identity = 300/350 (85.71%), Postives = 324/350 (92.57%), Query Frame = 0


Query: 1   MEKSSSASSLGPGGLDISQAFFKPVSDASPPSTSKRNTKISVIGAGNVGMAIAQTILTQD 60
           M KS S SSLGPGGLD+SQAFFKP+   +PPS +KRNTKISVIG GNVGMAIAQTILTQD
Sbjct: 1   MHKSPSGSSLGPGGLDLSQAFFKPIQSTAPPSATKRNTKISVIGVGNVGMAIAQTILTQD 60

Query: 61  LADELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSVTAESDLCIVTAGARQIAG 120
           LADE+ LVDAK DKLRGEMLDLQHAAAFLPRTKI+AS DYSVTA SDLCIVTAGARQ  G
Sbjct: 61  LADEIALVDAKEDKLRGEMLDLQHAAAFLPRTKINASVDYSVTAGSDLCIVTAGARQNPG 120

Query: 121 ESRLNLLQRNVALFQKIVPPLVQYSPETILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT 180
           ESRLNLLQRNVALF KI+PPLVQYSP+TILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT
Sbjct: 121 ESRLNLLQRNVALFSKIIPPLVQYSPDTILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT 180

Query: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLQNQQITYE 240
           NLDSSRFRFLIADHLDVNAQDVQAYI+GEHGDSSVALWSSIS+GGVP+LSFL+NQQI YE
Sbjct: 181 NLDSSRFRFLIADHLDVNAQDVQAYIIGEHGDSSVALWSSISIGGVPVLSFLKNQQIVYE 240

Query: 241 KETLESIHKEVIGGAYEVINLKGYTSWAIGYSVASLARSLLRDQRRIHPVSVLAKGFYGI 300
           KETLE+IHKEV+  AYEVI+LKGYTSWAIGYS A+LARS+LRDQR+IHPVSVLAKGFYGI
Sbjct: 241 KETLENIHKEVVNSAYEVISLKGYTSWAIGYSAANLARSILRDQRKIHPVSVLAKGFYGI 300

Query: 301 NGGELFLSLPAQLGRGGVLGITNVHLTDEESGRLRDSANTILQVQTQLGI 351
           NGG++FLSLPA LGRGGVLG+TNVH T EE  RLR SA TIL+VQ+QLG+
Sbjct: 301 NGGDVFLSLPALLGRGGVLGVTNVHFTKEEEQRLRKSAETILEVQSQLGL 350

The following BLAST results are available for this feature:

BLAST of Carg23210-RA vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022922404.1	4.4e-190	100.00	L-lactate dehydrogenase A-like isoform X1 [Cucurbita moschata] >XP_022922405.1 L...	[more]
XP_022969721.1	2.8e-189	99.43	L-lactate dehydrogenase A-like [Cucurbita maxima]	[more]
XP_023549806.1	2.8e-189	99.71	L-lactate dehydrogenase A-like [Cucurbita pepo subsp. pepo]	[more]
XP_008437100.2	1.6e-179	94.00	PREDICTED: L-lactate dehydrogenase A [Cucumis melo]	[more]
XP_004151663.2	2.3e-178	93.14	PREDICTED: LOW QUALITY PROTEIN: L-lactate dehydrogenase A-like [Cucumis sativus]	[more]

BLAST of Carg23210-RA vs. TAIR10


Match Name	E-value	Identity	Description
AT4G17260.1	1.1e-155	76.77	Lactate/malate dehydrogenase family protein	[more]

BLAST of Carg23210-RA vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|P22988\|LDHA_HORVU	5.4e-131	70.69	L-lactate dehydrogenase A OS=Hordeum vulgare OX=4513 PE=1 SV=1	[more]
sp\|P22989\|LDHB_HORVU	4.3e-128	68.71	L-lactate dehydrogenase B (Fragment) OS=Hordeum vulgare OX=4513 PE=1 SV=1	[more]
sp\|P29038\|LDH_MAIZE	1.2e-122	66.95	L-lactate dehydrogenase OS=Zea mays OX=4577 PE=2 SV=1	[more]
sp\|P06151\|LDHA_MOUSE	6.2e-95	56.77	L-lactate dehydrogenase A chain OS=Mus musculus OX=10090 GN=Ldha PE=1 SV=3	[more]
sp\|Q9XT87\|LDHA_MONDO	5.3e-94	55.45	L-lactate dehydrogenase A chain OS=Monodelphis domestica OX=13616 GN=LDHA PE=2 S...	[more]

BLAST of Carg23210-RA vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A1S3ATB9\|A0A1S3ATB9_CUCME	1.0e-179	94.00	L-lactate dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103482619 PE=3 SV=1	[more]
tr\|A0A0A0KKU9\|A0A0A0KKU9_CUCSA	1.1e-173	91.43	L-lactate dehydrogenase OS=Cucumis sativus OX=3659 GN=Csa_5G161920 PE=3 SV=1	[more]
tr\|A0A067KUG8\|A0A067KUG8_JATCU	1.1e-168	86.57	L-lactate dehydrogenase OS=Jatropha curcas OX=180498 GN=JCGZ_03382 PE=3 SV=1	[more]
tr\|A0A2P5F5D0\|A0A2P5F5D0_9ROSA	5.3e-168	86.00	L-lactate dehydrogenase OS=Trema orientalis OX=63057 GN=TorRG33x02_112750 PE=3 S...	[more]
tr\|A0A1R3GNR2\|A0A1R3GNR2_COCAP	4.5e-167	85.71	L-lactate dehydrogenase OS=Corchorus capsularis OX=210143 GN=CCACVL1_24669 PE=3 ...	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **Molecular Function**
Term	Definition
GO:0016491	oxidoreductase activity
GO:0003824	catalytic activity
GO:0004459	L-lactate dehydrogenase activity
GO:0016616	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor

Vocabulary: **Biological Process**
Term	Definition
GO:0005975	carbohydrate metabolic process
GO:0019752	carboxylic acid metabolic process
GO:0055114	oxidation-reduction process

Vocabulary: **Cellular Component**
Term	Definition
GO:0005737	cytoplasm

Vocabulary: **INTERPRO**
Term	Definition
IPR036291	NAD(P)-bd_dom_sf
IPR018177	L-lactate_DH_AS
IPR022383	Lactate/malate_DH_C
IPR001236	Lactate/malate_DH_N
IPR015955	Lactate_DH/Glyco_Ohase_4_C
IPR011304	L-lactate_DH
IPR001557	L-lactate/malate_DH

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
biological_process	GO:0019752	carboxylic acid metabolic process
biological_process	GO:0055114	oxidation-reduction process
biological_process	GO:0006534	cysteine metabolic process
biological_process	GO:0006094	gluconeogenesis
biological_process	GO:0006096	glycolytic process
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0003824	catalytic activity
molecular_function	GO:0004459	L-lactate dehydrogenase activity
molecular_function	GO:0016491	oxidoreductase activity
molecular_function	GO:0016616	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Carg23210	Carg23210	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg23210-RA.exon.1	Carg23210-RA.exon.1	exon
Carg23210-RA.exon.2	Carg23210-RA.exon.2	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg23210-RA.CDS.1	Carg23210-RA.CDS.1	CDS
Carg23210-RA.CDS.2	Carg23210-RA.CDS.2	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Carg23210-RA	Carg23210-RA-protein	polypeptide

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001557	L-lactate/malate dehydrogenase	PRINTS	PR00086	LLDHDRGNASE	coord: 39..63 score: 50.38 coord: 64..88 score: 51.92 coord: 205..218 score: 67.82 coord: 175..193 score: 68.42 coord: 151..171 score: 71.81
IPR001557	L-lactate/malate dehydrogenase	PIRSF	PIRSF000102	Lac_mal_DH	coord: 34..350 e-value: 3.6E-103 score: 342.9
IPR011304	L-lactate dehydrogenase	TIGRFAM	TIGR01771	TIGR01771	coord: 42..341 e-value: 7.7E-116 score: 384.6
IPR011304	L-lactate dehydrogenase	HAMAP	MF_00488	Lactate_dehydrog	coord: 37..349 score: 37.14
IPR015955	Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminal	GENE3D	G3DSA:3.90.110.10		coord: 180..349 e-value: 8.8E-62 score: 209.6
IPR015955	Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminal	SUPERFAMILY	SSF56327	LDH C-terminal domain-like	coord: 178..347
IPR001236	Lactate/malate dehydrogenase, N-terminal	PFAM	PF00056	Ldh_1_N	coord: 38..177 e-value: 3.1E-44 score: 150.4
None	No IPR available	GENE3D	G3DSA:3.40.50.720		coord: 23..179 e-value: 2.3E-64 score: 217.9
None	No IPR available	PANTHER	PTHR43128	FAMILY NOT NAMED	coord: 5..350
None	No IPR available	PANTHER	PTHR43128:SF12	SUBFAMILY NOT NAMED	coord: 5..350
None	No IPR available	CDD	cd05293	LDH_1	coord: 35..347 e-value: 1.01485E-174 score: 490.193
IPR022383	Lactate/malate dehydrogenase, C-terminal	PFAM	PF02866	Ldh_1_C	coord: 180..343 e-value: 1.4E-24 score: 86.8
IPR018177	L-lactate dehydrogenase, active site	PROSITE	PS00064	L_LDH	coord: 207..213
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	SSF51735	NAD(P)-binding Rossmann-fold domains	coord: 26..177