Carg23197-RA (mRNA) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg23197-RA
Type	mRNA
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	VAN3-binding protein-like
Location	Cucurbita_argyrosperma_scaffold_144 : 307850 .. 311375 (-)
Sequence length	708

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAAAGGGTTACATCTATGGGCTAAAGAGTATGGAGGAAGATGAGGAGCTAAAAATGGTGTCTTCTTTGCCTTCAATTCCTCAGCCTCGAACTCCACGAGAGCCTATGGAGTTCTTGGCAAGGACTTGGAGTCTCTCGGCTTCTGAGATTACAAAAGCCCTTGCTCAAAAGCAGAAGCCACTTCACATTGAAAGGAGCCCCATCACAGTTCCCGAGGCTATTGTTGCCCCACAACTCGTAAGCTTTTGTAACGGCCTAACGGTAGCAAATATTGTCCTCTTTGTGCTTTCCCTTTCGGGCTTCCCTCAAGAATTTTAAAACGCATCTGCTAGGGAGAAGTATCCACACCCTTATAAAGAAGGCTTCGTTCGTCGTTCTCCTCCTTTATAGAGGTGGGATCTCAAGGTTTCCATATCGGTTGGGGAGGAGAAAGAGTGCCTGCGAGGATCATCGGGTGGGGAGGAGAACAAAACATTCTTTAGAAGATTATGGAAACTTCTCCCTACAGACGCATTTTAAAAATCTTGAGGAGAAGCCCGAAAGGAAAGGCCCAAAGATGACAGCATATTCATGGCAAGTTAAGTGTTTTTCAGCTCTGGTGTTGTGTTGTGCTGAGTGTTTGTTCTATTTCTTTTTACAGCCAGGAAAGATTGTGAGTGCTTGGAGGGTAGGATCATTTGGAAAATGGTTTAACTTTCCTCACAAAGAGGCTGGTAATAGTATTGTCAAAAAGAAGGACAGGGCAAGAATTGAAAATGCTAGAGTTCATTCTGCTTTATCTGTGACAGCACTTGCTGCCGCCTTGGCTGCCGTGGCCGCAGCAGAGAAGTCTGATTCCAAAATGGGCATGGCTCTGGCCTCAGCTACTGAGATTTTGGCATCACATTGCATAGAGATGGCTGAATTCTCTGGCGCTGACCATGAGCAGGTCAGTTCGGTTATCAGCTCAGCAGTTGATGTCCAGAGCCCTGGCGACCTGATGACCTTAACAGCTGCGGCTGCCACAGGCACTTGTTCTATCCTTTTTTGCTTCTCAATATCAGTTTGTTAGTTAGGAAACACAACTCTTCGCAACGGTATGATATTGTCCACTTTGAGCAAAGAAACCTCCCTCGAGGCCTACAGAGCCCTCGAACAGTCTCTCCTTAGTCGAGGCTCGACTCCTTCTCTAGAGTTCTCGAACAAAGTACACCCTTTGTTCGACACTTGAGCCACTTTTGACTACGCCTTCGATACTCACAACTTCTTTGTTCGACATTTGAGGATTCTATTGACTTGGCTAAGTTAAGGACATGACTCTGATACCATATTAGGAACCACGACTCTTCACAATGGTACGATATTGTTCACTTTGAGCATAAACTCTCGTGGCTTTGCTTTGGGCTTCCCCAAAAGGCCTCATACCAATGGAGATGTATTCCTTACTTATAAACCCATGATCATTCCCTAAATTAGCCAACGTGGGACTCTCTCCCAACAATCCTCCTCTCGAACAAAGTACACTATAGAGCCTCCCCTGAGGCCTATGAAGCCCTCAAATAACCTCCCCTTAATCGAGGCTCAACTCCTTCTCTAAAACCCTCAGACAAAGTACACTATTTGTTCGACTCTTGAGTCACTTTTGACTGCACCTTCAAGGCTCAAAACTTCTTTGTTCGACATTTGAAGATTCTATTGACATGGCTAGGTTAAGGGCATGACTTGATACCATATTAGGAACCACGACTCTCCATAATGGTATGATATTGCTCACTTTGAGCATAAGCTCTCATGGCTTTGCTTTGGGCTTCCCCATAAGGTCTCATACCAATGAAGATGTATTCCTTATTAAACCCATGATCATTCTCTAAATTAGCCAATGTAGGACTCCCTTCAAACAATCCTCGACACCGTTTAAGCTCATGGAAAAAAGCATATCTGGATCTTAGTTAATGCTGTATTGTTTTTTTACTACTAATCCAGCTTTGCGAGGAGAAGCCGCTTTCAGATCCAGATTACTGAAGGAAGGCCGAAAAATAGCTTCTGTGAGTCCTTATGATAGGATAATGGCTCAGAATCATTGGGTTACCGCTTTTAGTAGCCATATGGAGGAGCAGGAGCTTCCCTGTGTGGGTGAATTGCTGCAGTTTACACGGAAAGGTACAGCTTGTTTTCTTCACAAACTTCAATTTTTCTTACATTCTTACTCGAAGATGGATATATTGCATTAACAAGTCTCTAACACATGTCCAATAAGTGTCGGAGCGTCCAAATGTCCGACACGCGTCGAACATAAACATGCTAGCCAAACTAAAGTGTCTGTTCTTCTTAGCTGATTTATTTAAACATCAATCACTCTTGATGATAACAGAACTTTAATGATATGTCACTGGTTGAGCTGCTCATTCTGAATCACTTCTCGTTGTACTAACAGGAACCCTGCGATGGAAGGAAGTATCTGTCTACATCAACAAGAAATCTCAGGTGCTGAGAAACTGCGTAATAAAAAGTAGAATTATGATCCAAATTGTTGTTATGAACATATCAACTACGTGGTTTCAGGTTGTAGCATCGATTAAAAGCAAGCACGTTGGAGGGACTTTTTCCAAGAAAAACAAATGTATGCATCTAATATCATCATTACTTTCTTCGATCCTATTTGTCGTCTCACAAGACGACTCGTCTTAACTGTGCATCTATGATGTTCCACATTGGTTGGGGAGGAGAACAAACCACCATTTATAAGGGTGTAGAAACCTTCCCCTAGCAGACGCGTTTTAAAGCTTTGAGGGGAAGCCCGAAAGGGAAAGCCCAAAGAGGACAATATCTGCTAACGGTGGATCTGGGGCGTTACAAATGGTATTAGAGTCAGACACCAAACAATGTGTCAGCCTTCTCGCTGTTCCCCGAAGGGGGTAGACACGAGACGGTGTGCTAGTAAGGACGCTGGGCCCCAAAGGGGATGAATTTGGGGGCGGTCCCACATCGATTGGAAGAAGGAAAGAGTGTCAGTAAAGACGCTGGGCCCCGAAGGGGGTGGATTGTGATGTCCCACATTGGTTGGGGAGGAGAACAAACCACCATTTATAAGGGTGTAGAAACCTTCCCCTGACATACACGTTTTAAAGCCTTGAGGGGAAGCCCGAAAGGAAAAGCCCCAAAAAGGACATTATCTGCTAGCAGTAGATTTGGGGCATTACAGCATCTGTCCTATTATAGGTGTTGTTTACGGGCTCTGCGACGAAACATCTTCATGGCCATACGTAAGAAAGAGAGACATATTGAACGAGGTCTATTTCGGCTTGAAAACCGCGCAAGGTCTTCTAGAGTTCAAGTGCAAGAATGGCCATACGTAAGAAAGAGAGACATATTGAACGAGGTCTATTTCGGCTTGAAAACCGCGCAAGGTCTTCTAGAGTTCAAGTGCAAGAACAAGATCCATAAACAGAGCTGGGTTCAAGGGATTCAAAGCCTTCTTCATCGAGTTAACTGCATCGAAACAACGAGATCATTACAGATTTTGAGTTTTAATGAGAGTATATAA

mRNA sequence

ATGGAAAAGGGTTACATCTATGGGCTAAAGAGTATGGAGGAAGATGAGGAGCTAAAAATGGTGTCTTCTTTGCCTTCAATTCCTCAGCCTCGAACTCCACGAGAGCCTATGGAGTTCTTGGCAAGGACTTGGAGATCATTTGGAAAATGGTTTAACTTTCCTCACAAAGAGGCTGGTAATAGTATTGTCAAAAAGAAGGACAGGGCAAGAATTGAAAATGCTAGAGTTCATTCTGCTTTATCTGTGACAGCACTTGCTGCCGCCTTGGCTGCCGTGGCCGCAGCAGAGAAGTCTGATTCCAAAATGGGCATGGCTCTGGCCTCAGCTACTGAGATTTTGGCATCACATTGCATAGAGATGGCTGAATTCTCTGGCGCTGACCATGAGCAGAATCATTGGGTTACCGCTTTTAGTAGCCATATGGAGGAGCAGGAGCTTCCCTGTGTGGGTGAATTGCTGCAGTTTACACGGAAAGGAACCCTGCGATGGAAGGAAGTATCTGTCTACATCAACAAGAAATCTCAGAGAGACATATTGAACGAGGTCTATTTCGGCTTGAAAACCGCGCAAGGTCTTCTAGAGTTCAAGTGCAAGAACAAGATCCATAAACAGAGCTGGGTTCAAGGGATTCAAAGCCTTCTTCATCGAGTTAACTGCATCGAAACAACGAGATCATTACAGATTTTGAGTTTTAATGAGAGTATATAA

Coding sequence (CDS)

ATGGAAAAGGGTTACATCTATGGGCTAAAGAGTATGGAGGAAGATGAGGAGCTAAAAATGGTGTCTTCTTTGCCTTCAATTCCTCAGCCTCGAACTCCACGAGAGCCTATGGAGTTCTTGGCAAGGACTTGGAGATCATTTGGAAAATGGTTTAACTTTCCTCACAAAGAGGCTGGTAATAGTATTGTCAAAAAGAAGGACAGGGCAAGAATTGAAAATGCTAGAGTTCATTCTGCTTTATCTGTGACAGCACTTGCTGCCGCCTTGGCTGCCGTGGCCGCAGCAGAGAAGTCTGATTCCAAAATGGGCATGGCTCTGGCCTCAGCTACTGAGATTTTGGCATCACATTGCATAGAGATGGCTGAATTCTCTGGCGCTGACCATGAGCAGAATCATTGGGTTACCGCTTTTAGTAGCCATATGGAGGAGCAGGAGCTTCCCTGTGTGGGTGAATTGCTGCAGTTTACACGGAAAGGAACCCTGCGATGGAAGGAAGTATCTGTCTACATCAACAAGAAATCTCAGAGAGACATATTGAACGAGGTCTATTTCGGCTTGAAAACCGCGCAAGGTCTTCTAGAGTTCAAGTGCAAGAACAAGATCCATAAACAGAGCTGGGTTCAAGGGATTCAAAGCCTTCTTCATCGAGTTAACTGCATCGAAACAACGAGATCATTACAGATTTTGAGTTTTAATGAGAGTATATAA

Protein sequence

MEKGYIYGLKSMEEDEELKMVSSLPSIPQPRTPREPMEFLARTWRSFGKWFNFPHKEAGNSIVKKKDRARIENARVHSALSVTALAAALAAVAAAEKSDSKMGMALASATEILASHCIEMAEFSGADHEQNHWVTAFSSHMEEQELPCVGELLQFTRKGTLRWKEVSVYINKKSQRDILNEVYFGLKTAQGLLEFKCKNKIHKQSWVQGIQSLLHRVNCIETTRSLQILSFNESI

Homology

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BLAST of Carg23197-RA vs. NCBI nr
Match: XP_022922410.1 (VAN3-binding protein-like [Cucurbita moschata])

HSP 1 Score: 327.8 bits (839), Expect = 3.0e-86
Identity = 230/375 (61.33%), Postives = 230/375 (61.33%), Query Frame = 0


Query: 1   MEKGYIYGLKSMEEDEEXXXXXXXXXXXXXXXXXXXXXFLARTWR--------------- 60
           MEKGYIYGLKSMEEDEE   XXXXXXXXXXXXXXXXXXFLARTW                
Sbjct: 1   MEKGYIYGLKSMEEDEELKMXXXXXXXXXXXXXXXXXXFLARTWSLSASEITKALAQKQK 60

Query: 61  -------------------------------SFGKWFNFPHKEAGNSIVKKKDRARIENA 120
                                          SFGKWFNFPHKEAGNSIVKKKDRARIENA
Sbjct: 61  PLHIERSRITVPEAIVAPPLPGKIVSAWRVGSFGKWFNFPHKEAGNSIVKKKDRARIENA 120

Query: 121 RVHSALSVTAXXXXXXXXXXXXKSDSKMGMALASATEILASHCIEMAEFSGADHE----- 180
           RVHSALSVTAXXXXXXXXXXXXKSDSKMGMALASATEILASHCIEMAEFSGADHE     
Sbjct: 121 RVHSALSVTAXXXXXXXXXXXXKSDSKMGMALASATEILASHCIEMAEFSGADHEQVSSV 180

Query: 181 ----------------------------------------------------QNHWVTAF 236
                                                               QNHW TAF
Sbjct: 181 ISSAVDVESPGDLMTLTAAAATALRGEAAFRSRLLKEGRKIASVSPYDRIMAQNHWATAF 240

[Close]

BLAST of Carg23197-RA vs. NCBI nr
Match: XP_023551130.1 (VAN3-binding protein-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 323.9 bits (829), Expect = 4.4e-85
Identity = 209/375 (55.73%), Postives = 211/375 (56.27%), Query Frame = 0


Query: 1   MEKGYIYGLKSMEEDEEXXXXXXXXXXXXXXXXXXXXXFLARTWR--------------- 60
           MEKGYIYGLKSMEEDEE                     FLARTW                
Sbjct: 1   MEKGYIYGLKSMEEDEELKMVSSLPSIPQPQTPREPMEFLARTWSLSASAISKALAQKHK 60

Query: 61  -------------------------------SFGKWFNFPHKEAGNSIVKKKDRARIENA 120
                                          SFGKWF FPHKEAGNSIVKKKDRARIENA
Sbjct: 61  PLHIERCPITVPEAIVVPQLPGKIVSAWRVGSFGKWFQFPHKEAGNSIVKKKDRARIENA 120

Query: 121 RVHSALSVTAXXXXXXXXXXXXKSDSKMGMALASATEILASHCIEMAEFSGADHE----- 180
           RVHSALSVTAXXXXXXXXXXXXKSDSKMGMALASATEILASHC+EMAEFSGADHE     
Sbjct: 121 RVHSALSVTAXXXXXXXXXXXXKSDSKMGMALASATEILASHCVEMAEFSGADHEQVSSV 180

Query: 181 ----------------------------------------------------QNHWVTAF 236
                                                               QNHW TAF
Sbjct: 181 ISSAVDVQSPGDLMTLTAAAATALRGEAAFRSRLLKEGRKIASVSPYDRIMAQNHWATAF 240

[Close]

BLAST of Carg23197-RA vs. NCBI nr
Match: XP_022973016.1 (VAN3-binding protein-like [Cucurbita maxima])

HSP 1 Score: 310.1 bits (793), Expect = 6.6e-81
Identity = 193/375 (51.47%), Postives = 196/375 (52.27%), Query Frame = 0


Query: 1   MEKGYIYGLKSMEEDEEXXXXXXXXXXXXXXXXXXXXXFLARTWR--------------- 60
           MEKGYIYGLKSMEEDEE                     FLARTW                
Sbjct: 1   MEKGYIYGLKSMEEDEELKMVSSLPSIPQPQTPREPMEFLARTWSLSASEITKALAQKQK 60

Query: 61  -------------------------------SFGKWFNFPHKEAGNSIVKKKDRARIENA 120
                                          SFGKWF FPHKEAGNSIV KKDRARIENA
Sbjct: 61  PLHIERSPITVPEAIVAPQLPGKIVSAWRVGSFGKWFQFPHKEAGNSIV-KKDRARIENA 120

Query: 121 RVHSALSVTAXXXXXXXXXXXXKSDSKMGMALASATEILASHCIEMAEFSGADHE----- 180
           RVHSALSVTA            KSDSKMGMAL SATEILASHCIEMAEFSGADHE     
Sbjct: 121 RVHSALSVTALAAALAAVTAAEKSDSKMGMALVSATEILASHCIEMAEFSGADHEQVSSV 180

Query: 181 ----------------------------------------------------QNHWVTAF 236
                                                               QN W TAF
Sbjct: 181 ISSAVDVQSPGDLMTLTAAAATALRGEAAFRSRLLKEGRKIASVSPYDRIMAQNQWATAF 240

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BLAST of Carg23197-RA vs. NCBI nr
Match: XP_004152426.1 (PREDICTED: uncharacterized protein LOC101210879 [Cucumis sativus] >XP_011654816.1 PREDICTED: uncharacterized protein LOC101210879 [Cucumis sativus] >XP_011654817.1 PREDICTED: uncharacterized protein LOC101210879 [Cucumis sativus] >KGN50261.1 hypothetical protein Csa_5G162590 [Cucumis sativus])

HSP 1 Score: 270.4 bits (690), Expect = 5.8e-69
Identity = 191/390 (48.97%), Postives = 200/390 (51.28%), Query Frame = 0


Query: 1   MEKGY--------IYGLKSMEEDEEXXXXXXXXXXXXXXXXXXXXXFLARTWR------- 60
           MEKGY        I+GLKSM EDEE                     FLAR+W        
Sbjct: 1   MEKGYCSSRKLGSIHGLKSMVEDEEMKMVSSFPSIPQPQTPQEPMEFLARSWSLSASEIT 60

Query: 61  ------------------------------------------SFGKWFNFPHKEAGNSIV 120
                                                     SFGKWFNFPHKEAGNSIV
Sbjct: 61  KALAQKQKQLYIERSPVTIPETIVAPQLPEKMVNSVHAWRVGSFGKWFNFPHKEAGNSIV 120

Query: 121 KKKDRARIENARVHSALS---VTAXXXXXXXXXXXXKSDSKMGMALASATEILASHCIEM 180
           KKKDRARIENARVHSA+S      XXXXXXXXXXXX    KMG ALASATEILASHCIEM
Sbjct: 121 KKKDRARIENARVHSAISXXXXXXXXXXXXXXXXXXXXXXKMGAALASATEILASHCIEM 180

Query: 181 AEFSGADHE--------------------------------------------------- 236
           AEF+GADHE                                                   
Sbjct: 181 AEFAGADHERVGSVIRSAVDVRSPGDLMTLTAAAATALRGEAAFRSRLPKEGRKIASVSP 240

[Close]

BLAST of Carg23197-RA vs. NCBI nr
Match: XP_022957682.1 (VAN3-binding protein [Cucurbita moschata])

HSP 1 Score: 268.1 bits (684), Expect = 2.9e-68
Identity = 171/292 (58.56%), Postives = 181/292 (61.99%), Query Frame = 0


Query: 44  WR--SFGKWFNFPHKEAGNSIVKKKDRARIENARVHSALSVTAXXXXXXXXXXXXKS--- 103
           WR  S GKWF+F HKE GNS+ KKKDRARIENARVHSALS   XXXXXXXXXXXX S   
Sbjct: 99  WRMGSLGKWFHFHHKEGGNSVDKKKDRARIENARVHSALSXXXXXXXXXXXXXXXNSAGP 158

Query: 104 DSKMGMALASATEILASHCIEMAEFSGADHE----------------------------- 163
           DS+MG ALASATEILASHCIEMAEF+GADHE                             
Sbjct: 159 DSRMGTALASATEILASHCIEMAEFAGADHERVSSVIRSAVDVRSPGDLMTLTAAAATAL 218

Query: 164 ----------------------------QNHWVTAFSSHMEEQELPCVGELLQFTRKGTL 223
                                       QNHW TAF+SHMEEQELPCVGELLQFT+KGTL
Sbjct: 219 RGEAAFRSRLPKEGRKIASVSPYDRIMAQNHWATAFNSHMEEQELPCVGELLQFTQKGTL 278

Query: 224 RWKEVSVYINKKSQ-------------------------------------RDILNEVYF 236
           RWKEVSVYINKKSQ                                     RDI NE+YF
Sbjct: 279 RWKEVSVYINKKSQVIASIKSKHVGGTFSKKNKCVVYGLCDETSSWPYERKRDISNEIYF 338

[Close]

BLAST of Carg23197-RA vs. TAIR10
Match: AT4G16670.1 (Plant protein of unknown function (DUF828) with plant pleckstrin homology-like region)

HSP 1 Score: 129.4 bits (324), Expect = 2.9e-30
Identity = 97/304 (31.91%), Postives = 132/304 (43.42%), Query Frame = 0


Query: 41  ARTWRSFGKWFNFPHKEAGN-SIVKKKDRARIENARVHSALSVTAXXXXXXXXXXXXKSD 100
           AR   + GKWF+      G  S VKK+DR R+E A +HSA+S+ +              D
Sbjct: 127 ARRTGTIGKWFHHREFVGGKVSAVKKRDRVRVEKAHLHSAVSIASLATAIAAVTASGNQD 186

Query: 101 ----SKMGMALASATEILASHCIEMAEFSGADHEQNHWVTAFSSHME------------- 160
               SKM  ALASA+E+LASHC+E+AE +GADH++   V+A  S ++             
Sbjct: 187 GFAGSKMSSALASASELLASHCLELAELAGADHDR--VVSAVRSAVDVRGPGDLLTLTAA 246

Query: 161 ----------------------------EQELP--------------------------C 220
                                       E+ LP                           
Sbjct: 247 AATALRGEAALRVRLPKEAKNSAAISPCERVLPETHSCSSELDRTSTTDELISAKGVEES 306

Query: 221 VGELLQFTRKGTLRWKEVSVYINKKSQ------------------RDILN---------- 235
            GEL+Q TR G LRWK V VYINKKSQ                  + I+N          
Sbjct: 307 TGELMQCTRNGVLRWKHVKVYINKKSQVIVEIKSKHVVGAFSMKSKGIVNDVCETVSGLQ 366

[Close]

BLAST of Carg23197-RA vs. TAIR10
Match: AT5G47440.1 (Plant protein of unknown function (DUF828) with plant pleckstrin homology-like region)

HSP 1 Score: 122.5 bits (306), Expect = 3.5e-28
Identity = 108/288 (37.50%), Postives = 132/288 (45.83%), Query Frame = 0


Query: 49  KWFNFPHKEAGN----SIVKKKDRARIENARVHSALSVTA----XXXXXXXXXXXXKSDS 108
           KWF+  HK+  N     I +KKD+AR++ A VHSA+S+ A          XXXXXX    
Sbjct: 117 KWFH--HKQHTNPSTMRIPRKKDKARVQKAHVHSAVSIAALAAGLASVTSXXXXXXXXXX 176

Query: 109 KMGMALASATEILASHCIEMAEFSGADH-------------------------------- 168
              +ALASATE+LASHCI+MAE +GADH                                
Sbjct: 177 XXALALASATELLASHCIDMAEQAGADHTCVASTVRSSVDIHSPGDLMTLTAAAATALRG 236

Query: 169 ------------EQNHWVT----AFS-SH---------MEEQELPCVGELLQFTRKGTLR 228
                        +N  +T    +FS SH         +EE  LP  GEL+Q  R G  R
Sbjct: 237 EAALKVRQPKESRKNATITPCERSFSDSHWPGENCQFRLEEPNLPLEGELVQCARNGLQR 296

Query: 229 WKEVSVYINKKSQ-------------------------------------RDILNEVYFG 233
            K V VYINKKSQ                                     R+   EVYFG
Sbjct: 297 NKRVCVYINKKSQVMIKLKSKHVGGAFSKKIKCVVYGVCDEISAWPCRKERENSEEVYFG 356

[Close]

BLAST of Carg23197-RA vs. TAIR10
Match: AT4G17350.1 (Plant protein of unknown function (DUF828) with plant pleckstrin homology-like region)

HSP 1 Score: 112.8 bits (281), Expect = 2.8e-25
Identity = 74/210 (35.24%), Postives = 85/210 (40.48%), Query Frame = 0


Query: 109 ATEILASHCIEMAEFSGADH---------------------------------------- 168
           ATE+LASHC+EMAE +GADH                                        
Sbjct: 184 ATELLASHCVEMAERAGADHARVTSTVRSSVDIHSPGDLMTLTAAAATALRGEAALKARQ 243

Query: 169 -----------------EQNHWVTAFSSHMEEQELPCVGELLQFTRKGTLRWKEVSVYIN 225
                              +HW   F   +EE  LP  GEL+Q  R G  R K V VYIN
Sbjct: 244 PKEARKNAAITPFERSFSDSHWHANFQYRLEEPNLPFEGELMQCARNGVQRTKRVCVYIN 303

[Close]

BLAST of Carg23197-RA vs. TAIR10
Match: AT4G32785.1 (Pleckstrin-like, plant (InterPro:IPR013666), Pleckstrin homology (InterPro:IPR001849))

HSP 1 Score: 58.5 bits (140), Expect = 6.2e-09
Identity = 39/116 (33.62%), Postives = 55/116 (47.41%), Query Frame = 0


Query: 151 ELLQFTRKGTLRWKEVSVYINK------------------KSQRDILNEV---------- 210
           ELL+ TRKG L WK+VS  IN                   K+++ ++N V          
Sbjct: 9   ELLKRTRKGDLHWKQVSFNINSNWQVVLKMKSKHVGGTFTKTKKCVVNGVCRDIPEWAHR 68

Query: 211 -----------YFGLKTAQGLLEFKCKNKIHKQSWVQGIQSLLHRVNCIETTRSLQ 228
                      YFG+KT + ++EF+C NK  KQ W++GIQ LL   N +E   S+Q
Sbjct: 69  GRADKMVERRAYFGVKTVERVIEFECGNKREKQMWIEGIQQLL---NSLEIGSSVQ 121

[Close]

BLAST of Carg23197-RA vs. TAIR10
Match: AT3G22810.1 (Plant protein of unknown function (DUF828) with plant pleckstrin homology-like region)

HSP 1 Score: 56.2 bits (134), Expect = 3.1e-08
Identity = 37/117 (31.62%), Postives = 53/117 (45.30%), Query Frame = 0


Query: 137 FSSHMEEQELPCVGELLQFTRKGTLRWKEVSVYINKKSQ--------------------- 196
           F  H   + L   G+LL+ TRKG L WK VSVYIN+ +Q                     
Sbjct: 344 FLGHCNREWLARGGQLLKRTRKGDLHWKIVSVYINRLNQVILKMKSRHVGGTFTKKNKNV 403

Query: 197 -RDILNEV----------------YFGLKTA-QGLLEFKCKNKIHKQSWVQGIQSLL 215
             D++  V                YFGLKT  +G++EF+CK++   + W QG+  L+
Sbjct: 404 VIDVIKNVQAWPGRHLLEGGEDLRYFGLKTVPRGIVEFQCKSQREYEMWTQGVSRLI 460

[Close]

BLAST of Carg23197-RA vs. Swiss-Prot
Match: sp|Q8W4K5|VAB_ARATH (VAN3-binding protein OS=Arabidopsis thaliana OX=3702 GN=VAB PE=1 SV=1)

HSP 1 Score: 52.0 bits (123), Expect = 1.1e-05
Identity = 39/110 (35.45%), Postives = 49/110 (44.55%), Query Frame = 0


Query: 144 QELPCVG-ELLQFTRKGTLRWKEVSVYINKKSQ--------------------------- 203
           QEL   G ELL+ TR G L WK VSVYINK  Q                           
Sbjct: 371 QELLAKGTELLKRTRGGELHWKIVSVYINKAGQAVLKMKSKHVGGTFTKKKKHMVLEVRK 430

Query: 204 -------RDILN---EVYFGLKT-AQGLLEFKCKNKIHKQSWVQGIQSLL 215
                  RD+ N     YFGLKT  + ++EF+C+N+   + W QG+  LL
Sbjct: 431 DIPAWAGRDLFNGDKHHYFGLKTETKRVIEFECRNQREYEIWTQGVSRLL 480

[Close]

BLAST of Carg23197-RA vs. TrEMBL
Match: tr|A0A0A0KRB0|A0A0A0KRB0_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G162590 PE=4 SV=1)

HSP 1 Score: 270.4 bits (690), Expect = 3.8e-69
Identity = 191/390 (48.97%), Postives = 200/390 (51.28%), Query Frame = 0


Query: 1   MEKGY--------IYGLKSMEEDEEXXXXXXXXXXXXXXXXXXXXXFLARTWR------- 60
           MEKGY        I+GLKSM EDEE                     FLAR+W        
Sbjct: 1   MEKGYCSSRKLGSIHGLKSMVEDEEMKMVSSFPSIPQPQTPQEPMEFLARSWSLSASEIT 60

Query: 61  ------------------------------------------SFGKWFNFPHKEAGNSIV 120
                                                     SFGKWFNFPHKEAGNSIV
Sbjct: 61  KALAQKQKQLYIERSPVTIPETIVAPQLPEKMVNSVHAWRVGSFGKWFNFPHKEAGNSIV 120

Query: 121 KKKDRARIENARVHSALS---VTAXXXXXXXXXXXXKSDSKMGMALASATEILASHCIEM 180
           KKKDRARIENARVHSA+S      XXXXXXXXXXXX    KMG ALASATEILASHCIEM
Sbjct: 121 KKKDRARIENARVHSAISXXXXXXXXXXXXXXXXXXXXXXKMGAALASATEILASHCIEM 180

Query: 181 AEFSGADHE--------------------------------------------------- 236
           AEF+GADHE                                                   
Sbjct: 181 AEFAGADHERVGSVIRSAVDVRSPGDLMTLTAAAATALRGEAAFRSRLPKEGRKIASVSP 240

[Close]

BLAST of Carg23197-RA vs. TrEMBL
Match: tr|A0A1S3ASR8|A0A1S3ASR8_CUCME (VAN3-binding protein-like OS=Cucumis melo OX=3656 GN=LOC103482602 PE=4 SV=1)

HSP 1 Score: 222.6 bits (566), Expect = 9.1e-55
Identity = 154/292 (52.74%), Postives = 166/292 (56.85%), Query Frame = 0


Query: 44  WR--SFGKWFNFPHKEAGNSIVKKKDRARIENARVHSALSVTAXXXXXXXXXXXXKSDSK 103
           WR  SFGKWF+FPHKEAGNSIVKKKDRARIENARVHSA+SV  XXXXXXXXXXXX S   
Sbjct: 94  WRVGSFGKWFHFPHKEAGNSIVKKKDRARIENARVHSAISVXXXXXXXXXXXXXXNSGGS 153

Query: 104 -------------------------------------------------MGMALASATEI 163
                                                              +  A+AT +
Sbjct: 154 DXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTLTAAAATAL 213

Query: 164 ---------LASHCIEMAEFSGADH--EQNHWVTAFSSHMEEQELPCVGELLQFTRKGTL 223
                    L     ++A  S  D    QNHW TAF+SHMEEQELPCVGELLQFTRKGTL
Sbjct: 214 RGEAAFRSRLPKEGRKIASVSPYDRITAQNHWTTAFNSHMEEQELPCVGELLQFTRKGTL 273

Query: 224 RWKEVSVYINKKSQ-------------------------------------RDILNEVYF 236
           RWKEVSVYINKKSQ                                     RDI NE+YF
Sbjct: 274 RWKEVSVYINKKSQVIASIKSKHVGGAFSKKNKCVVYGLCDETSSWPYERKRDISNEIYF 333

[Close]

BLAST of Carg23197-RA vs. TrEMBL
Match: tr|A0A2H5NTU3|A0A2H5NTU3_CITUN (Uncharacterized protein OS=Citrus unshiu OX=55188 GN=CUMW_075430 PE=4 SV=1)

HSP 1 Score: 179.9 bits (455), Expect = 6.8e-42
Identity = 132/294 (44.90%), Postives = 154/294 (52.38%), Query Frame = 0


Query: 41  ARTWRSFGKWFNFPHKEAGNSIVKKKDRARIENARVHSALSVT----AXXXXXXXXXXXX 100
           AR   S GKWF+  H+E   S VKKKDRAR+ENAR+HSALSV      XXXXXXXXXXXX
Sbjct: 98  ARRTGSIGKWFHH-HRELSTSKVKKKDRARVENARMHSALSVAXXXXXXXXXXXXXXXXX 157

Query: 101 KSDSKMGMALASATEILASHCIEMAEFSGADHE--------------------------- 160
              SKM MAL+SATE+LASHCIE+AE +GADH+                           
Sbjct: 158 XXGSKMSMALSSATELLASHCIELAESAGADHDRVASVVRSAVDIQTPGDLMTLTAAAAT 217

Query: 161 ------------------------------QNHWVTAFSSHMEEQELPCVGELLQFTRKG 220
                                         + HW  AF S +E+Q  PC GEL+Q TRKG
Sbjct: 218 ALRGEAALKARLPKEARKNASISPYEREVAETHWPAAFRSQLEKQRYPCEGELMQLTRKG 277

Query: 221 TLRWKEVSVYINKKSQ-------------------------------------RDILNEV 236
            LR K VSVYINKKSQ                                     R+I  EV
Sbjct: 278 VLRCKFVSVYINKKSQVIVKLKSKHVGGAFSKKNKCIVYGVCDESAAWPYKKEREISEEV 337

[Close]

BLAST of Carg23197-RA vs. TrEMBL
Match: tr|A0A2I4EIP6|A0A2I4EIP6_9ROSI (VAN3-binding protein OS=Juglans regia OX=51240 GN=LOC108989939 PE=4 SV=1)

HSP 1 Score: 179.5 bits (454), Expect = 8.9e-42
Identity = 132/291 (45.36%), Postives = 151/291 (51.89%), Query Frame = 0


Query: 40  LARTWRSFGKWFNFPHKEAGNSIVKKKDRARIENARVHSALSVTA---XXXXXXXXXXXX 99
           +AR   SFGKWF+  HKE+  S+VKKKDRAR+E+A +HS LSV     XXXXXXXXXXXX
Sbjct: 97  VARRTGSFGKWFH--HKESSISLVKKKDRARLESAHMHSTLSVAGLAXXXXXXXXXXXXX 156

Query: 100 KSDSKMGMALASATEILASHCIEMAEFSGADHE--------------------------- 159
               KM MALASATE+LASHCIE+AE +GA H+                           
Sbjct: 157 XXXKKMSMALASATELLASHCIELAELAGAGHDCVASVVRSAVEVQTPGDLMTLTAAAAT 216

Query: 160 ------------------------------QNHWVTAFSSHMEEQELPCVGELLQFTRKG 219
                                            W   F S M EQ  P VGELLQ T+KG
Sbjct: 217 ALRGEAALKARLPKEAKKNASISPYDRGMIGTDWSAGFHSRMVEQSPPFVGELLQVTQKG 276

Query: 220 TLRWKEVSVYINKKSQ-------------------------------------RDILNEV 233
            LRWK VSVYINKKSQ                                     R+I +EV
Sbjct: 277 VLRWKHVSVYINKKSQVIIKLKSKHVGGAFSKKNKCVVYGVCDETAAWPYRKEREISDEV 336

[Close]

BLAST of Carg23197-RA vs. TrEMBL
Match: tr|B9SXD6|B9SXD6_RICCO (Uncharacterized protein OS=Ricinus communis OX=3988 GN=RCOM_1388540 PE=4 SV=1)

HSP 1 Score: 175.3 bits (443), Expect = 1.7e-40
Identity = 131/294 (44.56%), Postives = 157/294 (53.40%), Query Frame = 0


Query: 41  ARTWRSFGKWFNFPHKEAGNSIVKKKDRARIENARVHSALSV---TAXXXXXXXXXXXXK 100
           +R   S GKWF+  HKE  +S VKKKD+AR+ENA +HSA+SV     XXXXXXXXXXXX 
Sbjct: 96  SRKTGSIGKWFH--HKELSSSTVKKKDKARMENAHMHSAISVXXXXXXXXXXXXXXXXXX 155

Query: 101 SDSKMGMALASATEILASHCIEMAEFSGADHEQ--------------------------- 160
              KMGMALASATE+LASHCIE+AE +GADH++                           
Sbjct: 156 XXXKMGMALASATELLASHCIELAESAGADHDRVASVVRSAVDIHSPGDLMTLTAAAATA 215

Query: 161 ------------------------------NHWVTAFSS-HMEEQELPCVGELLQFTRKG 220
                                           W +A+SS  +E Q  PC GEL+Q TRKG
Sbjct: 216 LRGEAALRARLPKEAKKNAAISPCDRGMADTSWDSAYSSGEVEAQAPPCNGELMQHTRKG 275

Query: 221 TLRWKEVSVYINKKS-------------------------------------QRDILNEV 236
            LRWK +SVYINKKS                                     +R+   +V
Sbjct: 276 VLRWKLISVYINKKSEVIIKIKSKHVGGAFSKKHKCIVYGVCDETTAWPYRKERESSEDV 335

The following BLAST results are available for this feature:

BLAST of Carg23197-RA vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022922410.1	3.0e-86	61.33	VAN3-binding protein-like [Cucurbita moschata]	[more]
XP_023551130.1	4.4e-85	55.73	VAN3-binding protein-like [Cucurbita pepo subsp. pepo]	[more]
XP_022973016.1	6.6e-81	51.47	VAN3-binding protein-like [Cucurbita maxima]	[more]
XP_004152426.1	5.8e-69	48.97	PREDICTED: uncharacterized protein LOC101210879 [Cucumis sativus] >XP_011654816....	[more]
XP_022957682.1	2.9e-68	58.56	VAN3-binding protein [Cucurbita moschata]	[more]

BLAST of Carg23197-RA vs. TAIR10


Match Name	E-value	Identity	Description
AT4G16670.1	2.9e-30	31.91	Plant protein of unknown function (DUF828) with plant pleckstrin homology-like r...	[more]
AT5G47440.1	3.5e-28	37.50	Plant protein of unknown function (DUF828) with plant pleckstrin homology-like r...	[more]
AT4G17350.1	2.8e-25	35.24	Plant protein of unknown function (DUF828) with plant pleckstrin homology-like r...	[more]
AT4G32785.1	6.2e-09	33.62	Pleckstrin-like, plant (InterPro:IPR013666), Pleckstrin homology (InterPro:IPR00...	[more]
AT3G22810.1	3.1e-08	31.62	Plant protein of unknown function (DUF828) with plant pleckstrin homology-like r...	[more]

BLAST of Carg23197-RA vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q8W4K5\|VAB_ARATH	1.1e-05	35.45	VAN3-binding protein OS=Arabidopsis thaliana OX=3702 GN=VAB PE=1 SV=1	[more]

BLAST of Carg23197-RA vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A0A0KRB0\|A0A0A0KRB0_CUCSA	3.8e-69	48.97	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G162590 PE=4 SV=1	[more]
tr\|A0A1S3ASR8\|A0A1S3ASR8_CUCME	9.1e-55	52.74	VAN3-binding protein-like OS=Cucumis melo OX=3656 GN=LOC103482602 PE=4 SV=1	[more]
tr\|A0A2H5NTU3\|A0A2H5NTU3_CITUN	6.8e-42	44.90	Uncharacterized protein OS=Citrus unshiu OX=55188 GN=CUMW_075430 PE=4 SV=1	[more]
tr\|A0A2I4EIP6\|A0A2I4EIP6_9ROSI	8.9e-42	45.36	VAN3-binding protein OS=Juglans regia OX=51240 GN=LOC108989939 PE=4 SV=1	[more]
tr\|B9SXD6\|B9SXD6_RICCO	1.7e-40	44.56	Uncharacterized protein OS=Ricinus communis OX=3988 GN=RCOM_1388540 PE=4 SV=1	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **INTERPRO**
Term	Definition
IPR008546	DUF828
IPR013666	PH_pln

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003674	molecular_function

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Carg23197	Carg23197	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Carg23197-RA	Carg23197-RA-protein	polypeptide

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg23197-RA.CDS.5	Carg23197-RA.CDS.5	CDS
Carg23197-RA.CDS.4	Carg23197-RA.CDS.4	CDS
Carg23197-RA.CDS.3	Carg23197-RA.CDS.3	CDS
Carg23197-RA.CDS.2	Carg23197-RA.CDS.2	CDS
Carg23197-RA.CDS.1	Carg23197-RA.CDS.1	CDS

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg23197-RA.exon.5	Carg23197-RA.exon.5	exon
Carg23197-RA.exon.4	Carg23197-RA.exon.4	exon
Carg23197-RA.exon.3	Carg23197-RA.exon.3	exon
Carg23197-RA.exon.2	Carg23197-RA.exon.2	exon
Carg23197-RA.exon.1	Carg23197-RA.exon.1	exon

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013666	Pleckstrin-like, plant	PFAM	PF08458	PH_2	coord: 181..217 e-value: 6.0E-11 score: 42.5 coord: 151..175 e-value: 3.6E-5 score: 23.9
IPR008546	Domain of unknown function DUF828	PFAM	PF05703	Auxin_canalis	coord: 46..131 e-value: 1.5E-19 score: 70.8
None	No IPR available	PANTHER	PTHR31351:SF2	SUBFAMILY NOT NAMED	coord: 180..234 coord: 130..175
None	No IPR available	PANTHER	PTHR31351:SF2	SUBFAMILY NOT NAMED	coord: 46..130 coord: 6..44
None	No IPR available	PANTHER	PTHR31351	FAMILY NOT NAMED	coord: 46..130 coord: 6..44
None	No IPR available	PANTHER	PTHR31351	FAMILY NOT NAMED	coord: 180..234 coord: 130..175