Carg21840-RA (mRNA) Silver-seed gourd

NameCarg21840-RA
TypemRNA
OrganismCucurbita argyrosperma (Silver-seed gourd)
Descriptionvignain-like
LocationCucurbita_argyrosperma_scaffold_192 : 216021 .. 217367 (+)
Sequence length1002
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCATTAGCAAATTTCTTTTGGTTCCTTTTCTTTTGATTGTTCTAGTTTCAGGATTGGGTGAAAGCTTTGAATTCGATGAGAAGGAGTTGGCAACAGAGGAAAGTCTATGGCGGCTGTATGAGAGGTGGGGTAACCACCACACTATCTCAAGGGGCCTAAAGGAGAAGCATAAACGTTTCAACGTGTTTAAAGAGAACGTGAACCATGTGTTCACAGTGAACCAAATGAACAAACCATACAAGCTGAAGCTGAACAAGTTCGCGGATATGTCCAACTCTGAGTTTGTGAGCTTCTATGCTCGCTCCAATATTAGCCACTACAGGAAGTTACATGGCAAAAGATCAGGTGGATTCATGTATGAGCAGGCTACAGATCTTCCATCATACATCGACTGGAGGGAAAGAGGAGCTGTCAATGACATCAAAGAGCAAGGCAGATGTGGTATAATTTCTAATTCTGGCTAGACAACTCAACACAACGTTTAAGTGTTTTGAGAGTTCTCACAACTAGATTATGAAAATGTAAAGCATTGGATGTCAATGGAAATCATAAATAACCAATGGATTATGAAATATATTGCAGGAAGCTGTTGGGCGTTTTCATCTGTGGCTGCAGTTGAAGGAATCAACCAAATCAAAACCAACCGGCTACTATCTCTATCAGAGCAGGAGCTGCTCGACTGCAATATTAGGAACAGAGGCTGTAACGGAGGATTCATGGAGATCGCATTCGATTTCATAAAGCGAAATGGTGGAATCGCCACTGAGAACAGCTATCCGTACCACGGCGCAAGAGGATTGTGTCGCTCATCCAGAGTCAGTTTTATAATTGAATTCAAAACTAAAATATATGGATCCTTTCAGAAATGATCAAACTAAGCATCTACTTCTTTGGTTTAGCAGATATCTTCACCGACAGTAACAATCGATGGATATGAAAGCGTACCTGAAAACGAGAATGCCCTGATGCAAGCCGTCGCAAACCAACCAGTCTCAGTCGCCATCGACGCCGTGGGAAGAGATTTCCAATTCTACTCGCAGGCAAGTTTGTGGTTCATTCGAGAAATCGATTGCTTCACTATAATATCTTGAGGCATTGTTAATCTTAAAACCGTGGGAAAACTGCAGGGAGTGTTCGATGGATACTGTGGAACGGAGCTGAATCACGGAGTGGTGGCGATTGGTTACGGAACAACAGAAGAGGGAACAGATTACTGGATCGTGAGGAACTCGTGGGGAGTTGGATGGGGAGAAGAAGGTTATGTAAGGATGAAGCGAGGAGTGGAGCAGTCGGAAGGGCTGTGTGGAATAGTCATGGAAGCCTCTTATCCCATCAAATACTAG

mRNA sequence

ATGGCCATTAGCAAATTTCTTTTGGTTCCTTTTCTTTTGATTGTTCTAGTTTCAGGATTGGGTGAAAGCTTTGAATTCGATGAGAAGGAGTTGGCAACAGAGGAAAGTCTATGGCGGCTGTATGAGAGGTGGGGTAACCACCACACTATCTCAAGGGGCCTAAAGGAGAAGCATAAACGTTTCAACGTGTTTAAAGAGAACGTGAACCATGTGTTCACAGTGAACCAAATGAACAAACCATACAAGCTGAAGCTGAACAAGTTCGCGGATATGTCCAACTCTGAGTTTGTGAGCTTCTATGCTCGCTCCAATATTAGCCACTACAGGAAGTTACATGGCAAAAGATCAGGTGGATTCATGTATGAGCAGGCTACAGATCTTCCATCATACATCGACTGGAGGGAAAGAGGAGCTGTCAATGACATCAAAGAGCAAGGCAGATGTGGAAGCTGTTGGGCGTTTTCATCTGTGGCTGCAGTTGAAGGAATCAACCAAATCAAAACCAACCGGCTACTATCTCTATCAGAGCAGGAGCTGCTCGACTGCAATATTAGGAACAGAGGCTGTAACGGAGGATTCATGGAGATCGCATTCGATTTCATAAAGCGAAATGGTGGAATCGCCACTGAGAACAGCTATCCGTACCACGGCGCAAGAGGATTGTGTCGCTCATCCAGAATATCTTCACCGACAGTAACAATCGATGGATATGAAAGCGTACCTGAAAACGAGAATGCCCTGATGCAAGCCGTCGCAAACCAACCAGTCTCAGTCGCCATCGACGCCGGAGTGTTCGATGGATACTGTGGAACGGAGCTGAATCACGGAGTGGTGGCGATTGGTTACGGAACAACAGAAGAGGGAACAGATTACTGGATCGTGAGGAACTCGTGGGGAGTTGGATGGGGAGAAGAAGGTTATGTAAGGATGAAGCGAGGAGTGGAGCAGTCGGAAGGGCTGTGTGGAATAGTCATGGAAGCCTCTTATCCCATCAAATACTAG

Coding sequence (CDS)

ATGGCCATTAGCAAATTTCTTTTGGTTCCTTTTCTTTTGATTGTTCTAGTTTCAGGATTGGGTGAAAGCTTTGAATTCGATGAGAAGGAGTTGGCAACAGAGGAAAGTCTATGGCGGCTGTATGAGAGGTGGGGTAACCACCACACTATCTCAAGGGGCCTAAAGGAGAAGCATAAACGTTTCAACGTGTTTAAAGAGAACGTGAACCATGTGTTCACAGTGAACCAAATGAACAAACCATACAAGCTGAAGCTGAACAAGTTCGCGGATATGTCCAACTCTGAGTTTGTGAGCTTCTATGCTCGCTCCAATATTAGCCACTACAGGAAGTTACATGGCAAAAGATCAGGTGGATTCATGTATGAGCAGGCTACAGATCTTCCATCATACATCGACTGGAGGGAAAGAGGAGCTGTCAATGACATCAAAGAGCAAGGCAGATGTGGAAGCTGTTGGGCGTTTTCATCTGTGGCTGCAGTTGAAGGAATCAACCAAATCAAAACCAACCGGCTACTATCTCTATCAGAGCAGGAGCTGCTCGACTGCAATATTAGGAACAGAGGCTGTAACGGAGGATTCATGGAGATCGCATTCGATTTCATAAAGCGAAATGGTGGAATCGCCACTGAGAACAGCTATCCGTACCACGGCGCAAGAGGATTGTGTCGCTCATCCAGAATATCTTCACCGACAGTAACAATCGATGGATATGAAAGCGTACCTGAAAACGAGAATGCCCTGATGCAAGCCGTCGCAAACCAACCAGTCTCAGTCGCCATCGACGCCGGAGTGTTCGATGGATACTGTGGAACGGAGCTGAATCACGGAGTGGTGGCGATTGGTTACGGAACAACAGAAGAGGGAACAGATTACTGGATCGTGAGGAACTCGTGGGGAGTTGGATGGGGAGAAGAAGGTTATGTAAGGATGAAGCGAGGAGTGGAGCAGTCGGAAGGGCTGTGTGGAATAGTCATGGAAGCCTCTTATCCCATCAAATACTAG

Protein sequence

MAISKFLLVPFLLIVLVSGLGESFEFDEKELATEESLWRLYERWGNHHTISRGLKEKHKRFNVFKENVNHVFTVNQMNKPYKLKLNKFADMSNSEFVSFYARSNISHYRKLHGKRSGGFMYEQATDLPSYIDWRERGAVNDIKEQGRCGSCWAFSSVAAVEGINQIKTNRLLSLSEQELLDCNIRNRGCNGGFMEIAFDFIKRNGGIATENSYPYHGARGLCRSSRISSPTVTIDGYESVPENENALMQAVANQPVSVAIDAGVFDGYCGTELNHGVVAIGYGTTEEGTDYWIVRNSWGVGWGEEGYVRMKRGVEQSEGLCGIVMEASYPIKY
BLAST of Carg21840-RA vs. NCBI nr
Match: XP_023545499.1 (vignain-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 654.4 bits (1687), Expect = 2.0e-184
Identity = 325/372 (87.37%), Postives = 331/372 (88.98%), Query Frame = 0

Query: 1   MAISKFLLVPFLLIVLVSGLGESFEFDEKELATEESLWRLYERWGNHHTISRGLKEKHKR 60
           MAISKFLLVPFLLIVLVSGLGESFEFDEKELATEESLWRLYERWGNHHTISRGLKEKHKR
Sbjct: 28  MAISKFLLVPFLLIVLVSGLGESFEFDEKELATEESLWRLYERWGNHHTISRGLKEKHKR 87

Query: 61  FNVFKENVNHVFTVNQMNKPYKLKLNKFADMSNSEFVSFYARSNISHYRKLHGKRSGGFM 120
           FNVFKENVNHVFTVNQMNKPYKLKLNKFADMSNSEFVSFYARSNISHYRKLHGKRSGGFM
Sbjct: 88  FNVFKENVNHVFTVNQMNKPYKLKLNKFADMSNSEFVSFYARSNISHYRKLHGKRSGGFM 147

Query: 121 YEQATDLPSYIDWRERGAVNDIKEQGRCGSCWAFSSVAAVEGINQIKTNRLLSLSEQELL 180
           YEQATDLPSYIDWRERGAVNDIKEQG+CGSCWAFSSVAAVEGINQIKTN+LLSLSEQELL
Sbjct: 148 YEQATDLPSYIDWRERGAVNDIKEQGKCGSCWAFSSVAAVEGINQIKTNQLLSLSEQELL 207

Query: 181 DCNIRNRGCNGGFMEIAFDFIKRNGGIATENSYPYHGARGLCRSSRISSPTVTIDGYESV 240
           DCN RN+GCNGGFMEIAFDFIK+NGGIATEN+YPYHGARGLCRSSR SSPTVTIDGYESV
Sbjct: 208 DCNFRNKGCNGGFMEIAFDFIKKNGGIATENNYPYHGARGLCRSSRKSSPTVTIDGYESV 267

Query: 241 PENENALMQAVANQPVSVAIDA-------------------------------------- 300
           PENENALMQAVANQPVSVAIDA                                      
Sbjct: 268 PENENALMQAVANQPVSVAIDAVGRDFQFYSQASLWLIRAIDCFTIISSGIVNLKTVGKL 327

Query: 301 -GVFDGYCGTELNHGVVAIGYGTTEEGTDYWIVRNSWGVGWGEEGYVRMKRGVEQSEGLC 334
            GVFDGYCGTELNHGVVAIGYGTTEEGTDYW+VRNSWGVGWGEEGYVRMKRGVEQSEGLC
Sbjct: 328 QGVFDGYCGTELNHGVVAIGYGTTEEGTDYWMVRNSWGVGWGEEGYVRMKRGVEQSEGLC 387

BLAST of Carg21840-RA vs. NCBI nr
Match: XP_022996765.1 (vignain-like [Cucurbita maxima])

HSP 1 Score: 653.3 bits (1684), Expect = 4.5e-184
Identity = 323/373 (86.60%), Postives = 332/373 (89.01%), Query Frame = 0

Query: 1   MAISKFLLVPFLLIVLVSGLGESFEFDEKELATEESLWRLYERWGNHHTISRGLKEKHKR 60
           MAISKF+LVPFLLIVLVSGLGESFEFDEKELATEESLWRLYERWGNHHTISRGLKEKHKR
Sbjct: 1   MAISKFILVPFLLIVLVSGLGESFEFDEKELATEESLWRLYERWGNHHTISRGLKEKHKR 60

Query: 61  FNVFKENVNHVFTVNQMNKPYKLKLNKFADMSNSEFVSFYARSNISHYRKLHGKRSGGFM 120
           FNVFKENVNHVFTVNQMNKPYKLKLNKFADMSNSEFVSFYARSNISHYRKLHGKRSGGFM
Sbjct: 61  FNVFKENVNHVFTVNQMNKPYKLKLNKFADMSNSEFVSFYARSNISHYRKLHGKRSGGFM 120

Query: 121 YEQATDLPSYIDWRERGAVNDIKEQGRCGSCWAFSSVAAVEGINQIKTNRLLSLSEQELL 180
           YEQATDLPSYIDWRERGAVNDIKEQG+CGSCWAFS+VAAVEGINQIKTN+LLSLSEQELL
Sbjct: 121 YEQATDLPSYIDWRERGAVNDIKEQGKCGSCWAFSTVAAVEGINQIKTNQLLSLSEQELL 180

Query: 181 DCNIRNRGCNGGFMEIAFDFIKRNGGIATENSYPYHGARGLCRSSRISSPTVTIDGYESV 240
           DCN RN+GCNGGFMEIAFDFIKRNGGIATEN+YPYHGARGLCRSSRISSPTVTIDGYESV
Sbjct: 181 DCNFRNKGCNGGFMEIAFDFIKRNGGIATENNYPYHGARGLCRSSRISSPTVTIDGYESV 240

Query: 241 PENENALMQAVANQPVSVAIDA-------------------------------------- 300
           PENENALMQAVANQPVSVAIDA                                      
Sbjct: 241 PENENALMQAVANQPVSVAIDAVGRDFQFYSQASLGFIREIDWLTIIISSGNVNLKSVGK 300

Query: 301 --GVFDGYCGTELNHGVVAIGYGTTEEGTDYWIVRNSWGVGWGEEGYVRMKRGVEQSEGL 334
             GVFDGYCGTELNHGVVAIGYGTTEEGTDYW+VRNSWGVGWGEEGYVRMKRGVE++EGL
Sbjct: 301 VQGVFDGYCGTELNHGVVAIGYGTTEEGTDYWMVRNSWGVGWGEEGYVRMKRGVEKAEGL 360

BLAST of Carg21840-RA vs. NCBI nr
Match: XP_004142960.1 (PREDICTED: vignain-like [Cucumis sativus] >KGN62334.1 hypothetical protein Csa_2G349680 [Cucumis sativus])

HSP 1 Score: 617.8 bits (1592), Expect = 2.1e-173
Identity = 302/345 (87.54%), Postives = 319/345 (92.46%), Query Frame = 0

Query: 1   MAISKFLLVPFLLIVLVSGLGESFEFDEKELATEESLWRLYERWGNHHTISRGLKEKHKR 60
           MAI KFLLVP LLIVLVSGL ESFEFDEKELATEESLW+LYERWG HHTISR LKEKHKR
Sbjct: 1   MAIGKFLLVPLLLIVLVSGLAESFEFDEKELATEESLWQLYERWGKHHTISRNLKEKHKR 60

Query: 61  FNVFKENVNHVFTVNQMNKPYKLKLNKFADMSNSEFVSFYARSNISHYRKLHGKR--SGG 120
           F+VFKENVNHVFTVNQM+KPYKLKLNKFADMSN EFV+FYARSNISHYRKLH +R  +GG
Sbjct: 61  FSVFKENVNHVFTVNQMDKPYKLKLNKFADMSNYEFVNFYARSNISHYRKLHERRRGAGG 120

Query: 121 FMYEQATDLPSYIDWRERGAVNDIKEQGRCGSCWAFSSVAAVEGINQIKTNRLLSLSEQE 180
           FMYEQ TDLPS +DWRERGAVN +KEQGRCGSCWAFSSVAAVEGIN+IKTN+LLSLSEQE
Sbjct: 121 FMYEQDTDLPSSVDWRERGAVNAVKEQGRCGSCWAFSSVAAVEGINKIKTNQLLSLSEQE 180

Query: 181 LLDCNIRNRGCNGGFMEIAFDFIKRNGGIATENSYPYHGARGLCRSSRISSPTVTIDGYE 240
           LLDCN RN+GCNGGFMEIAFDFIKRNGGIATENSYPYHG+RGLCRSSRISSP V IDGYE
Sbjct: 181 LLDCNYRNKGCNGGFMEIAFDFIKRNGGIATENSYPYHGSRGLCRSSRISSPIVKIDGYE 240

Query: 241 SVPENENALMQAVANQPVSVAIDA----------GVFDGYCGTELNHGVVAIGYGTTEEG 300
           SVPENE+ALMQAVANQPVSVAIDA          GVFDGYCGTELNHGVVAIGYGTTE+G
Sbjct: 241 SVPENEDALMQAVANQPVSVAIDAAGRDFQFYSQGVFDGYCGTELNHGVVAIGYGTTEDG 300

Query: 301 TDYWIVRNSWGVGWGEEGYVRMKRGVEQSEGLCGIVMEASYPIKY 334
           TDYW+VRNSWGVGWGE+GYVRMKRGVEQ+EGLCGI MEASYPIKY
Sbjct: 301 TDYWLVRNSWGVGWGEDGYVRMKRGVEQAEGLCGIAMEASYPIKY 345

BLAST of Carg21840-RA vs. NCBI nr
Match: XP_008444390.1 (PREDICTED: vignain-like [Cucumis melo])

HSP 1 Score: 617.8 bits (1592), Expect = 2.1e-173
Identity = 303/345 (87.83%), Postives = 318/345 (92.17%), Query Frame = 0

Query: 1   MAISKFLLVPFLLIVLVSGLGESFEFDEKELATEESLWRLYERWGNHHTISRGLKEKHKR 60
           MAI KFLLVP LLIVLVSGL ESFEFDEKELATEESLW+LYERWGNHHTISR LKEKHKR
Sbjct: 1   MAIGKFLLVPLLLIVLVSGLAESFEFDEKELATEESLWQLYERWGNHHTISRNLKEKHKR 60

Query: 61  FNVFKENVNHVFTVNQMNKPYKLKLNKFADMSNSEFVSFYARSNISHYRKLHGKR--SGG 120
           F+VFKENVNHVFTVNQMNKPYKLKLNKFADMSN EFV+FYARSNISH+RKLHG+R  +GG
Sbjct: 61  FSVFKENVNHVFTVNQMNKPYKLKLNKFADMSNYEFVNFYARSNISHFRKLHGRRRGAGG 120

Query: 121 FMYEQATDLPSYIDWRERGAVNDIKEQGRCGSCWAFSSVAAVEGINQIKTNRLLSLSEQE 180
           FMYEQ TDLPS +DWRERGAVN IKEQG CGSCWAFSSVAAVE IN+IKTN+LLSLSEQE
Sbjct: 121 FMYEQDTDLPSSVDWRERGAVNAIKEQGTCGSCWAFSSVAAVEAINKIKTNQLLSLSEQE 180

Query: 181 LLDCNIRNRGCNGGFMEIAFDFIKRNGGIATENSYPYHGARGLCRSSRISSPTVTIDGYE 240
           LLDCN RN+GCNGGFMEIAFDFIKRNGGIATENSYPYHG+RGLCRSSRISSP V IDGYE
Sbjct: 181 LLDCNYRNKGCNGGFMEIAFDFIKRNGGIATENSYPYHGSRGLCRSSRISSPIVKIDGYE 240

Query: 241 SVPENENALMQAVANQPVSVAIDA----------GVFDGYCGTELNHGVVAIGYGTTEEG 300
           SVPENE+ALMQAVANQPVSVAIDA          GVFDGYCGTELNHGVVAIGYGTTE+G
Sbjct: 241 SVPENEDALMQAVANQPVSVAIDAAGRDFQFYWQGVFDGYCGTELNHGVVAIGYGTTEDG 300

Query: 301 TDYWIVRNSWGVGWGEEGYVRMKRGVEQSEGLCGIVMEASYPIKY 334
           TDYWIVRNSWGVGWGE+GYVRMKRGVEQ EGLCGI MEASYPIK+
Sbjct: 301 TDYWIVRNSWGVGWGEDGYVRMKRGVEQPEGLCGIAMEASYPIKF 345

BLAST of Carg21840-RA vs. NCBI nr
Match: XP_023537428.1 (vignain-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 571.2 bits (1471), Expect = 2.2e-159
Identity = 278/346 (80.35%), Postives = 309/346 (89.31%), Query Frame = 0

Query: 1   MAISKFLLVPFLLIVLVSGLGESFEFDEKELATEESLWRLYERWGNHHTISRGLKEKHKR 60
           MAISK LLVPF LIVLVSGL +SFEFDE+ELAT+ SLW+LYERW +HH ISR LKEKHKR
Sbjct: 1   MAISKCLLVPF-LIVLVSGLAKSFEFDEEELATDGSLWKLYERWSHHHAISRELKEKHKR 60

Query: 61  FNVFKENVNHVFTVNQMNKPYKLKLNKFADMSNSEFVSFYARSNISHYRKLHGKR---SG 120
           +NVFKEN NHV TVNQMNKPYKLKLNKFADMSN EFV+ YARSNI+HYR+LHGKR   + 
Sbjct: 61  YNVFKENANHVLTVNQMNKPYKLKLNKFADMSNYEFVNLYARSNITHYRRLHGKRREGAS 120

Query: 121 GFMYEQATDLPSYIDWRERGAVNDIKEQGRCGSCWAFSSVAAVEGINQIKTNRLLSLSEQ 180
           GFMYE+ATDLPS+IDWRERGAV+DIK+QGRCGSCWAFS+VAAVEGINQIKTN+LLSLSEQ
Sbjct: 121 GFMYEKATDLPSFIDWRERGAVSDIKDQGRCGSCWAFSAVAAVEGINQIKTNQLLSLSEQ 180

Query: 181 ELLDCNIRNRGCNGGFMEIAFDFIKRNGGIATENSYPYHGARGLCRSSRISSPTVTIDGY 240
           ELLDCN RNRGC GGFME A++FI+RNGGIA+EN+YPY GARG CRSSR+ SP VTIDG+
Sbjct: 181 ELLDCNTRNRGCYGGFMETAYNFIRRNGGIASENNYPYRGARGSCRSSRMPSPIVTIDGF 240

Query: 241 ESVPENENALMQAVANQPVSVAIDA----------GVFDGYCGTELNHGVVAIGYGTTEE 300
           ESVPENENALMQAVANQPVSV+I+A          GVFDG CGTELNHGVV IGYGTT++
Sbjct: 241 ESVPENENALMQAVANQPVSVSIEALGRDFQFYWQGVFDGDCGTELNHGVVVIGYGTTDD 300

Query: 301 GTDYWIVRNSWGVGWGEEGYVRMKRGVEQSEGLCGIVMEASYPIKY 334
           GTDYW VRNSWGVGWGE+GY+RMKRGVE  EGLCGIVMEASYP+K+
Sbjct: 301 GTDYWSVRNSWGVGWGEDGYIRMKRGVEDPEGLCGIVMEASYPLKF 345

BLAST of Carg21840-RA vs. TAIR10
Match: AT3G48350.1 (Cysteine proteinases superfamily protein)

HSP 1 Score: 409.1 bits (1050), Expect = 2.7e-114
Identity = 205/347 (59.08%), Postives = 257/347 (74.06%), Query Frame = 0

Query: 1   MAISKFLLVPFLLIVLVSGLGESFEFDEKELATEESLWRLYERWGNHHTISRGLKEKHKR 60
           M +   +L+ FL ++  S   + F+FDEKEL TEE++W+LYERW  HH++SR   E  KR
Sbjct: 1   MKLFFIVLISFLSLLQAS---KGFDFDEKELETEENVWKLYERWRGHHSVSRASHEAIKR 60

Query: 61  FNVFKENVNHVFTVNQMNKPYKLKLNKFADMSNSEFVSFYARSNISHYRKLHGKR--SGG 120
           FNVF+ NV HV   N+ NKPYKLK+N+FAD+++ EF S YA SN+ H+R L G +  SGG
Sbjct: 61  FNVFRHNVLHVHRTNKKNKPYKLKINRFADITHHEFRSSYAGSNVKHHRMLRGPKRGSGG 120

Query: 121 FMYEQATDLPSYIDWRERGAVNDIKEQGRCGSCWAFSSVAAVEGINQIKTNRLLSLSEQE 180
           FMYE  T +PS +DWRE+GAV ++K Q  CGSCWAFS+VAAVEGIN+I+TN+L+SLSEQE
Sbjct: 121 FMYENVTRVPSSVDWREKGAVTEVKNQQDCGSCWAFSTVAAVEGINKIRTNKLVSLSEQE 180

Query: 181 LLDCNI-RNRGCNGGFMEIAFDFIKRNGGIATENSYPYHGAR-GLCRSSRISSPTVTIDG 240
           L+DC+   N+GC GG ME AF+FIK NGGI TE +YPY  +    CR++ I   TVTIDG
Sbjct: 181 LVDCDTEENQGCAGGLMEPAFEFIKNNGGIKTEETYPYDSSDVQFCRANSIGGETVTIDG 240

Query: 241 YESVPEN-ENALMQAVANQPVSVAIDA----------GVFDGYCGTELNHGVVAIGYGTT 300
           +E VPEN E  L++AVA+QPVSVAIDA          GVF G CGT+LNHGVV +GYG T
Sbjct: 241 HEHVPENDEEELLKAVAHQPVSVAIDAGSSDFQLYSEGVFIGECGTQLNHGVVIVGYGET 300

Query: 301 EEGTDYWIVRNSWGVGWGEEGYVRMKRGVEQSEGLCGIVMEASYPIK 333
           + GT YWIVRNSWG  WGE GYVR++RG+ ++EG CGI MEASYP K
Sbjct: 301 KNGTKYWIVRNSWGPEWGEGGYVRIERGISENEGRCGIAMEASYPTK 344

BLAST of Carg21840-RA vs. TAIR10
Match: AT3G48340.1 (Cysteine proteinases superfamily protein)

HSP 1 Score: 407.1 bits (1045), Expect = 1.0e-113
Identity = 203/344 (59.01%), Postives = 257/344 (74.71%), Query Frame = 0

Query: 5   KFLLVPFLLIVLVSGLGESFEFDEKELATEESLWRLYERWGNHHTISRGLKEKHKRFNVF 64
           K LL+ FL  +++      F++D+KE+ +EE L  LY+RW +HH++ R L E+ KRFNVF
Sbjct: 2   KKLLLIFLFSLVILQTACGFDYDDKEIESEEGLSTLYDRWRSHHSVPRSLNEREKRFNVF 61

Query: 65  KENVNHVFTVNQMNKPYKLKLNKFADMSNSEFVSFYARSNISHYRKLHGKRSGG--FMY- 124
           + NV HV   N+ N+ YKLKLNKFAD++ +EF + Y  SNI H+R L G + G   FMY 
Sbjct: 62  RHNVMHVHNTNKKNRSYKLKLNKFADLTINEFKNAYTGSNIKHHRMLQGPKRGSKQFMYD 121

Query: 125 -EQATDLPSYIDWRERGAVNDIKEQGRCGSCWAFSSVAAVEGINQIKTNRLLSLSEQELL 184
            E  + LPS +DWR++GAV +IK QG+CGSCWAFS+VAAVEGIN+IKTN+L+SLSEQEL+
Sbjct: 122 HENLSKLPSSVDWRKKGAVTEIKNQGKCGSCWAFSTVAAVEGINKIKTNKLVSLSEQELV 181

Query: 185 DCNIR-NRGCNGGFMEIAFDFIKRNGGIATENSYPYHGARGLCRSSRISSPTVTIDGYES 244
           DC+ + N GCNGG MEIAF+FIK+NGGI TE+SYPY G  G C +S+ +   VTIDG+E 
Sbjct: 182 DCDTKQNEGCNGGLMEIAFEFIKKNGGITTEDSYPYEGIDGKCDASKDNGVLVTIDGHED 241

Query: 245 VPEN-ENALMQAVANQPVSVAIDA----------GVFDGYCGTELNHGVVAIGYGTTEEG 304
           VPEN ENAL++AVANQPVSVAIDA          GVF G CGTELNHGV A+GYG +E G
Sbjct: 242 VPENDENALLKAVANQPVSVAIDAGSSDFQFYSEGVFTGSCGTELNHGVAAVGYG-SERG 301

Query: 305 TDYWIVRNSWGVGWGEEGYVRMKRGVEQSEGLCGIVMEASYPIK 333
             YWIVRNSWG  WGE GY++++R +++ EG CGI MEASYPIK
Sbjct: 302 KKYWIVRNSWGAEWGEGGYIKIEREIDEPEGRCGIAMEASYPIK 344

BLAST of Carg21840-RA vs. TAIR10
Match: AT5G50260.1 (Cysteine proteinases superfamily protein)

HSP 1 Score: 395.6 bits (1015), Expect = 3.1e-110
Identity = 191/342 (55.85%), Postives = 247/342 (72.22%), Query Frame = 0

Query: 5   KFLLVPFLLIVLVSGLGESFEFDEKELATEESLWRLYERWGNHHTISRGLKEKHKRFNVF 64
           K  +V  L +++V    +  +F  K++ +E SLW LYERW +HHT++R L+EK KRFNVF
Sbjct: 2   KRFIVLALCMLMVLETTKGLDFHNKDVESENSLWELYERWRSHHTVARSLEEKAKRFNVF 61

Query: 65  KENVNHVFTVNQMNKPYKLKLNKFADMSNSEFVSFYARSNISHYRKLHG--KRSGGFMYE 124
           K NV H+   N+ +K YKLKLNKF DM++ EF   YA SNI H+R   G  K +  FMY 
Sbjct: 62  KHNVKHIHETNKKDKSYKLKLNKFGDMTSEEFRRTYAGSNIKHHRMFQGEKKATKSFMYA 121

Query: 125 QATDLPSYIDWRERGAVNDIKEQGRCGSCWAFSSVAAVEGINQIKTNRLLSLSEQELLDC 184
               LP+ +DWR+ GAV  +K QG+CGSCWAFS+V AVEGINQI+T +L SLSEQEL+DC
Sbjct: 122 NVNTLPTSVDWRKNGAVTPVKNQGQCGSCWAFSTVVAVEGINQIRTKKLTSLSEQELVDC 181

Query: 185 NI-RNRGCNGGFMEIAFDFIKRNGGIATENSYPYHGARGLCRSSRISSPTVTIDGYESVP 244
           +  +N+GCNGG M++AF+FIK  GG+ +E  YPY  +   C +++ ++P V+IDG+E VP
Sbjct: 182 DTNQNQGCNGGLMDLAFEFIKEKGGLTSELVYPYKASDETCDTNKENAPVVSIDGHEDVP 241

Query: 245 EN-ENALMQAVANQPVSVAIDA----------GVFDGYCGTELNHGVVAIGYGTTEEGTD 304
           +N E+ LM+AVANQPVSVAIDA          GVF G CGTELNHGV  +GYGTT +GT 
Sbjct: 242 KNSEDDLMKAVANQPVSVAIDAGGSDFQFYSEGVFTGRCGTELNHGVAVVGYGTTIDGTK 301

Query: 305 YWIVRNSWGVGWGEEGYVRMKRGVEQSEGLCGIVMEASYPIK 333
           YWIV+NSWG  WGE+GY+RM+RG+   EGLCGI MEASYP+K
Sbjct: 302 YWIVKNSWGEEWGEKGYIRMQRGIRHKEGLCGIAMEASYPLK 343

BLAST of Carg21840-RA vs. TAIR10
Match: AT5G43060.1 (Granulin repeat cysteine protease family protein)

HSP 1 Score: 312.0 bits (798), Expect = 4.4e-85
Identity = 172/353 (48.73%), Postives = 223/353 (63.17%), Query Frame = 0

Query: 8   LVPFLLIVLVSGLGESFE-----FDEKELATEES------LWRLYERW----GNHHTISR 67
           L P +L++ + G+  + +     +DE    T E+      + R+YE W    G       
Sbjct: 6   LSPMILLLAMIGVSYAMDMSIISYDENHHITTETSRSDSEVERIYEAWMVEHGKKKMNQN 65

Query: 68  GL-KEKHKRFNVFKENVNHVFTVNQMNKPYKLKLNKFADMSNSEFVSFYARSNISHYRKL 127
           GL  EK +RF +FK+N+  +   N  N  YKL L +FAD++N E+ S Y  +  +   K 
Sbjct: 66  GLGAEKDQRFEIFKDNLRFIDEHNTKNLSYKLGLTRFADLTNEEYRSMYLGAKPT---KR 125

Query: 128 HGKRSGGFMYEQATDLPSYIDWRERGAVNDIKEQGRCGSCWAFSSVAAVEGINQIKTNRL 187
             K S  +       LP  +DWR+ GAV D+K+QG CGSCWAFS++ AVEGIN+I T  L
Sbjct: 126 VLKTSDRYQARVGDALPDSVDWRKEGAVADVKDQGSCGSCWAFSTIGAVEGINKIVTGDL 185

Query: 188 LSLSEQELLDCNIR-NRGCNGGFMEIAFDFIKRNGGIATENSYPYHGARGLCRSSRISSP 247
           +SLSEQEL+DC+   N+GCNGG M+ AF+FI +NGGI TE  YPY  A G C  +R ++ 
Sbjct: 186 ISLSEQELVDCDTSYNQGCNGGLMDYAFEFIIKNGGIDTEADYPYKAADGRCDQNRKNAK 245

Query: 248 TVTIDGYESVPEN-ENALMQAVANQPVSVAIDA----------GVFDGYCGTELNHGVVA 307
            VTID YE VPEN E +L +A+A+QP+SVAI+A          GVFDG CGTEL+HGVVA
Sbjct: 246 VVTIDSYEDVPENSEASLKKALAHQPISVAIEAGGRAFQLYSSGVFDGLCGTELDHGVVA 305

Query: 308 IGYGTTEEGTDYWIVRNSWGVGWGEEGYVRMKRGVEQSEGLCGIVMEASYPIK 333
           +GYG TE G DYWIVRNSWG  WGE GY++M R +E   G CGI MEASYPIK
Sbjct: 306 VGYG-TENGKDYWIVRNSWGNRWGESGYIKMARNIEAPTGKCGIAMEASYPIK 354

BLAST of Carg21840-RA vs. TAIR10
Match: AT1G47128.1 (Granulin repeat cysteine protease family protein)

HSP 1 Score: 307.0 bits (785), Expect = 1.4e-83
Identity = 166/351 (47.29%), Postives = 220/351 (62.68%), Query Frame = 0

Query: 7   LLVPFLLIVLVSGLGES--FEFDEKELA------TEESLWRLYERWGNHH---TISRGLK 66
           + + FL +V VS   +     +DEK         +E  +  +YE W   H        L 
Sbjct: 8   MAILFLAMVAVSSAVDMSIISYDEKHGVSTTGGRSEAEVMSIYEAWLVKHGKAQSQNSLV 67

Query: 67  EKHKRFNVFKENVNHVFTVNQMNKPYKLKLNKFADMSNSEFVSFYARSNISHYRKLHGKR 126
           EK +RF +FK+N+  V   N+ N  Y+L L +FAD++N E+ S Y  + +    +  G+R
Sbjct: 68  EKDRRFEIFKDNLRFVDEHNEKNLSYRLGLTRFADLTNDEYRSKYLGAKM----EKKGER 127

Query: 127 SGGFMYEQ--ATDLPSYIDWRERGAVNDIKEQGRCGSCWAFSSVAAVEGINQIKTNRLLS 186
                YE     +LP  IDWR++GAV ++K+QG CGSCWAFS++ AVEGINQI T  L++
Sbjct: 128 RTSLRYEARVGDELPESIDWRKKGAVAEVKDQGGCGSCWAFSTIGAVEGINQIVTGDLIT 187

Query: 187 LSEQELLDCNIR-NRGCNGGFMEIAFDFIKRNGGIATENSYPYHGARGLCRSSRISSPTV 246
           LSEQEL+DC+   N GCNGG M+ AF+FI +NGGI T+  YPY G  G C   R ++  V
Sbjct: 188 LSEQELVDCDTSYNEGCNGGLMDYAFEFIIKNGGIDTDKDYPYKGVDGTCDQIRKNAKVV 247

Query: 247 TIDGYESVPE-NENALMQAVANQPVSVAI----------DAGVFDGYCGTELNHGVVAIG 306
           TID YE VP  +E +L +AVA+QP+S+AI          D+G+FDG CGT+L+HGVVA+G
Sbjct: 248 TIDSYEDVPTYSEESLKKAVAHQPISIAIEAGGRAFQLYDSGIFDGSCGTQLDHGVVAVG 307

Query: 307 YGTTEEGTDYWIVRNSWGVGWGEEGYVRMKRGVEQSEGLCGIVMEASYPIK 333
           YG TE G DYWIVRNSWG  WGE GY+RM R +  S G CGI +E SYPIK
Sbjct: 308 YG-TENGKDYWIVRNSWGKSWGESGYLRMARNIASSSGKCGIAIEPSYPIK 353

BLAST of Carg21840-RA vs. Swiss-Prot
Match: sp|P12412|CYSEP_VIGMU (Vignain OS=Vigna mungo OX=3915 PE=1 SV=1)

HSP 1 Score: 433.0 bits (1112), Expect = 3.1e-120
Identity = 212/346 (61.27%), Postives = 265/346 (76.59%), Query Frame = 0

Query: 1   MAISKFLLVPFLLIVLVSGLGESFEFDEKELATEESLWRLYERWGNHHTISRGLKEKHKR 60
           MA+ K L V  L + LV G+  SF+F EK+L +EESLW LYERW +HHT+SR L EKHKR
Sbjct: 1   MAMKKLLWV-VLSLSLVLGVANSFDFHEKDLESEESLWDLYERWRSHHTVSRSLGEKHKR 60

Query: 61  FNVFKENVNHVFTVNQMNKPYKLKLNKFADMSNSEFVSFYARSNISHYRKLHGKR--SGG 120
           FNVFK NV HV   N+M+KPYKLKLNKFADM+N EF S YA S ++H++   G +  SG 
Sbjct: 61  FNVFKANVMHVHNTNKMDKPYKLKLNKFADMTNHEFRSTYAGSKVNHHKMFRGSQHGSGT 120

Query: 121 FMYEQATDLPSYIDWRERGAVNDIKEQGRCGSCWAFSSVAAVEGINQIKTNRLLSLSEQE 180
           FMYE+   +P+ +DWR++GAV D+K+QG+CGSCWAFS++ AVEGINQIKTN+L+SLSEQE
Sbjct: 121 FMYEKVGSVPASVDWRKKGAVTDVKDQGQCGSCWAFSTIVAVEGINQIKTNKLVSLSEQE 180

Query: 181 LLDCN-IRNRGCNGGFMEIAFDFIKRNGGIATENSYPYHGARGLCRSSRISSPTVTIDGY 240
           L+DC+   N+GCNGG ME AF+FIK+ GGI TE++YPY    G C  S+++   V+IDG+
Sbjct: 181 LVDCDKEENQGCNGGLMESAFEFIKQKGGITTESNYPYTAQEGTCDESKVNDLAVSIDGH 240

Query: 241 ESVPEN-ENALMQAVANQPVSVAIDA----------GVFDGYCGTELNHGVVAIGYGTTE 300
           E+VP N ENAL++AVANQPVSVAIDA          GVF G C T+LNHGV  +GYGTT 
Sbjct: 241 ENVPVNDENALLKAVANQPVSVAIDAGGSDFQFYSEGVFTGDCNTDLNHGVAIVGYGTTV 300

Query: 301 EGTDYWIVRNSWGVGWGEEGYVRMKRGVEQSEGLCGIVMEASYPIK 333
           +GT+YWIVRNSWG  WGE+GY+RM+R + + EGLCGI M ASYPIK
Sbjct: 301 DGTNYWIVRNSWGPEWGEQGYIRMQRNISKKEGLCGIAMMASYPIK 345

BLAST of Carg21840-RA vs. Swiss-Prot
Match: sp|O65039|CYSEP_RICCO (Vignain OS=Ricinus communis OX=3988 GN=CYSEP PE=1 SV=1)

HSP 1 Score: 429.5 bits (1103), Expect = 3.4e-119
Identity = 208/344 (60.47%), Postives = 263/344 (76.45%), Query Frame = 0

Query: 3   ISKFLLVPFLLIVLVSGLGESFEFDEKELATEESLWRLYERWGNHHTISRGLKEKHKRFN 62
           + KF+L+  L + LV  + ESF+F EKEL +EESLW LYERW +HHT+SR L EK KRFN
Sbjct: 1   MQKFILLA-LSLALVLAITESFDFHEKELESEESLWGLYERWRSHHTVSRSLHEKQKRFN 60

Query: 63  VFKENVNHVFTVNQMNKPYKLKLNKFADMSNSEFVSFYARSNISHYRKLHG--KRSGGFM 122
           VFK N  HV   N+M+KPYKLKLNKFADM+N EF + Y+ S + H+R   G  + +G FM
Sbjct: 61  VFKHNAMHVHNANKMDKPYKLKLNKFADMTNHEFRNTYSGSKVKHHRMFRGGPRGNGTFM 120

Query: 123 YEQATDLPSYIDWRERGAVNDIKEQGRCGSCWAFSSVAAVEGINQIKTNRLLSLSEQELL 182
           YE+   +P+ +DWR++GAV  +K+QG+CGSCWAFS++ AVEGINQIKTN+L+SLSEQEL+
Sbjct: 121 YEKVDTVPASVDWRKKGAVTSVKDQGQCGSCWAFSTIVAVEGINQIKTNKLVSLSEQELV 180

Query: 183 DCNI-RNRGCNGGFMEIAFDFIKRNGGIATENSYPYHGARGLCRSSRISSPTVTIDGYES 242
           DC+  +N+GCNGG M+ AF+FIK+ GGI TE +YPY    G C  S+ ++P V+IDG+E+
Sbjct: 181 DCDTDQNQGCNGGLMDYAFEFIKQRGGITTEANYPYEAYDGTCDVSKENAPAVSIDGHEN 240

Query: 243 VPEN-ENALMQAVANQPVSVAIDA----------GVFDGYCGTELNHGVVAIGYGTTEEG 302
           VPEN ENAL++AVANQPVSVAIDA          GVF G CGTEL+HGV  +GYGTT +G
Sbjct: 241 VPENDENALLKAVANQPVSVAIDAGGSDFQFYSEGVFTGSCGTELDHGVAIVGYGTTIDG 300

Query: 303 TDYWIVRNSWGVGWGEEGYVRMKRGVEQSEGLCGIVMEASYPIK 333
           T YW V+NSWG  WGE+GY+RM+RG+   EGLCGI MEASYPIK
Sbjct: 301 TKYWTVKNSWGPEWGEKGYIRMERGISDKEGLCGIAMEASYPIK 343

BLAST of Carg21840-RA vs. Swiss-Prot
Match: sp|P25803|CYSEP_PHAVU (Vignain OS=Phaseolus vulgaris OX=3885 PE=2 SV=2)

HSP 1 Score: 424.5 bits (1090), Expect = 1.1e-117
Identity = 209/346 (60.40%), Postives = 260/346 (75.14%), Query Frame = 0

Query: 1   MAISKFLLVPFLLIVLVSGLGESFEFDEKELATEESLWRLYERWGNHHTISRGLKEKHKR 60
           MA  K L V  L   LV G+  SF+F +K+LA+EESLW LYERW +HHT+SR L EKHKR
Sbjct: 1   MATKKLLWV-VLSFSLVLGVANSFDFHDKDLASEESLWDLYERWRSHHTVSRSLGEKHKR 60

Query: 61  FNVFKENVNHVFTVNQMNKPYKLKLNKFADMSNSEFVSFYARSNISHYRKLHG--KRSGG 120
           FNVFK N+ HV   N+M+KPYKLKLNKFADM+N EF S YA S ++H R   G    +G 
Sbjct: 61  FNVFKANLMHVHNTNKMDKPYKLKLNKFADMTNHEFRSTYAGSKVNHPRMFRGTPHENGA 120

Query: 121 FMYEQATDLPSYIDWRERGAVNDIKEQGRCGSCWAFSSVAAVEGINQIKTNRLLSLSEQE 180
           FMYE+   +P  +DWR++GAV D+K+QG+CGSCWAFS+V AVEGINQIKTN+L++LSEQE
Sbjct: 121 FMYEKVVSVPPSVDWRKKGAVTDVKDQGQCGSCWAFSTVVAVEGINQIKTNKLVALSEQE 180

Query: 181 LLDCN-IRNRGCNGGFMEIAFDFIKRNGGIATENSYPYHGARGLCRSSRISSPTVTIDGY 240
           L+DC+   N+GCNGG ME AF+FIK+ GGI TE++YPY    G C +S+++   V+IDG+
Sbjct: 181 LVDCDKEENQGCNGGLMESAFEFIKQKGGITTESNYPYKAQEGTCDASKVNDLAVSIDGH 240

Query: 241 ESVPEN-ENALMQAVANQPVSVAIDA----------GVFDGYCGTELNHGVVAIGYGTTE 300
           E+VP N E+AL++AVANQPVSVAIDA          GVF G C T+LNHGV  +GYGTT 
Sbjct: 241 ENVPANDEDALLKAVANQPVSVAIDAGGSDFQFYSEGVFTGDCSTDLNHGVAIVGYGTTV 300

Query: 301 EGTDYWIVRNSWGVGWGEEGYVRMKRGVEQSEGLCGIVMEASYPIK 333
           +GT+YWIVRNSWG  WGE GY+RM+R + + EGLCGI M  SYPIK
Sbjct: 301 DGTNYWIVRNSWGPEWGEHGYIRMQRNISKKEGLCGIAMLPSYPIK 345

BLAST of Carg21840-RA vs. Swiss-Prot
Match: sp|Q9STL5|CEP3_ARATH (KDEL-tailed cysteine endopeptidase CEP3 OS=Arabidopsis thaliana OX=3702 GN=CEP3 PE=2 SV=1)

HSP 1 Score: 409.1 bits (1050), Expect = 4.8e-113
Identity = 205/347 (59.08%), Postives = 257/347 (74.06%), Query Frame = 0

Query: 1   MAISKFLLVPFLLIVLVSGLGESFEFDEKELATEESLWRLYERWGNHHTISRGLKEKHKR 60
           M +   +L+ FL ++  S   + F+FDEKEL TEE++W+LYERW  HH++SR   E  KR
Sbjct: 1   MKLFFIVLISFLSLLQAS---KGFDFDEKELETEENVWKLYERWRGHHSVSRASHEAIKR 60

Query: 61  FNVFKENVNHVFTVNQMNKPYKLKLNKFADMSNSEFVSFYARSNISHYRKLHGKR--SGG 120
           FNVF+ NV HV   N+ NKPYKLK+N+FAD+++ EF S YA SN+ H+R L G +  SGG
Sbjct: 61  FNVFRHNVLHVHRTNKKNKPYKLKINRFADITHHEFRSSYAGSNVKHHRMLRGPKRGSGG 120

Query: 121 FMYEQATDLPSYIDWRERGAVNDIKEQGRCGSCWAFSSVAAVEGINQIKTNRLLSLSEQE 180
           FMYE  T +PS +DWRE+GAV ++K Q  CGSCWAFS+VAAVEGIN+I+TN+L+SLSEQE
Sbjct: 121 FMYENVTRVPSSVDWREKGAVTEVKNQQDCGSCWAFSTVAAVEGINKIRTNKLVSLSEQE 180

Query: 181 LLDCNI-RNRGCNGGFMEIAFDFIKRNGGIATENSYPYHGAR-GLCRSSRISSPTVTIDG 240
           L+DC+   N+GC GG ME AF+FIK NGGI TE +YPY  +    CR++ I   TVTIDG
Sbjct: 181 LVDCDTEENQGCAGGLMEPAFEFIKNNGGIKTEETYPYDSSDVQFCRANSIGGETVTIDG 240

Query: 241 YESVPEN-ENALMQAVANQPVSVAIDA----------GVFDGYCGTELNHGVVAIGYGTT 300
           +E VPEN E  L++AVA+QPVSVAIDA          GVF G CGT+LNHGVV +GYG T
Sbjct: 241 HEHVPENDEEELLKAVAHQPVSVAIDAGSSDFQLYSEGVFIGECGTQLNHGVVIVGYGET 300

Query: 301 EEGTDYWIVRNSWGVGWGEEGYVRMKRGVEQSEGLCGIVMEASYPIK 333
           + GT YWIVRNSWG  WGE GYVR++RG+ ++EG CGI MEASYP K
Sbjct: 301 KNGTKYWIVRNSWGPEWGEGGYVRIERGISENEGRCGIAMEASYPTK 344

BLAST of Carg21840-RA vs. Swiss-Prot
Match: sp|Q9STL4|CEP2_ARATH (KDEL-tailed cysteine endopeptidase CEP2 OS=Arabidopsis thaliana OX=3702 GN=CEP2 PE=1 SV=1)

HSP 1 Score: 407.1 bits (1045), Expect = 1.8e-112
Identity = 203/344 (59.01%), Postives = 257/344 (74.71%), Query Frame = 0

Query: 5   KFLLVPFLLIVLVSGLGESFEFDEKELATEESLWRLYERWGNHHTISRGLKEKHKRFNVF 64
           K LL+ FL  +++      F++D+KE+ +EE L  LY+RW +HH++ R L E+ KRFNVF
Sbjct: 2   KKLLLIFLFSLVILQTACGFDYDDKEIESEEGLSTLYDRWRSHHSVPRSLNEREKRFNVF 61

Query: 65  KENVNHVFTVNQMNKPYKLKLNKFADMSNSEFVSFYARSNISHYRKLHGKRSGG--FMY- 124
           + NV HV   N+ N+ YKLKLNKFAD++ +EF + Y  SNI H+R L G + G   FMY 
Sbjct: 62  RHNVMHVHNTNKKNRSYKLKLNKFADLTINEFKNAYTGSNIKHHRMLQGPKRGSKQFMYD 121

Query: 125 -EQATDLPSYIDWRERGAVNDIKEQGRCGSCWAFSSVAAVEGINQIKTNRLLSLSEQELL 184
            E  + LPS +DWR++GAV +IK QG+CGSCWAFS+VAAVEGIN+IKTN+L+SLSEQEL+
Sbjct: 122 HENLSKLPSSVDWRKKGAVTEIKNQGKCGSCWAFSTVAAVEGINKIKTNKLVSLSEQELV 181

Query: 185 DCNIR-NRGCNGGFMEIAFDFIKRNGGIATENSYPYHGARGLCRSSRISSPTVTIDGYES 244
           DC+ + N GCNGG MEIAF+FIK+NGGI TE+SYPY G  G C +S+ +   VTIDG+E 
Sbjct: 182 DCDTKQNEGCNGGLMEIAFEFIKKNGGITTEDSYPYEGIDGKCDASKDNGVLVTIDGHED 241

Query: 245 VPEN-ENALMQAVANQPVSVAIDA----------GVFDGYCGTELNHGVVAIGYGTTEEG 304
           VPEN ENAL++AVANQPVSVAIDA          GVF G CGTELNHGV A+GYG +E G
Sbjct: 242 VPENDENALLKAVANQPVSVAIDAGSSDFQFYSEGVFTGSCGTELNHGVAAVGYG-SERG 301

Query: 305 TDYWIVRNSWGVGWGEEGYVRMKRGVEQSEGLCGIVMEASYPIK 333
             YWIVRNSWG  WGE GY++++R +++ EG CGI MEASYPIK
Sbjct: 302 KKYWIVRNSWGAEWGEGGYIKIEREIDEPEGRCGIAMEASYPIK 344

BLAST of Carg21840-RA vs. TrEMBL
Match: tr|A0A1S3BA70|A0A1S3BA70_CUCME (vignain-like OS=Cucumis melo OX=3656 GN=LOC103487731 PE=3 SV=1)

HSP 1 Score: 617.8 bits (1592), Expect = 1.4e-173
Identity = 303/345 (87.83%), Postives = 318/345 (92.17%), Query Frame = 0

Query: 1   MAISKFLLVPFLLIVLVSGLGESFEFDEKELATEESLWRLYERWGNHHTISRGLKEKHKR 60
           MAI KFLLVP LLIVLVSGL ESFEFDEKELATEESLW+LYERWGNHHTISR LKEKHKR
Sbjct: 1   MAIGKFLLVPLLLIVLVSGLAESFEFDEKELATEESLWQLYERWGNHHTISRNLKEKHKR 60

Query: 61  FNVFKENVNHVFTVNQMNKPYKLKLNKFADMSNSEFVSFYARSNISHYRKLHGKR--SGG 120
           F+VFKENVNHVFTVNQMNKPYKLKLNKFADMSN EFV+FYARSNISH+RKLHG+R  +GG
Sbjct: 61  FSVFKENVNHVFTVNQMNKPYKLKLNKFADMSNYEFVNFYARSNISHFRKLHGRRRGAGG 120

Query: 121 FMYEQATDLPSYIDWRERGAVNDIKEQGRCGSCWAFSSVAAVEGINQIKTNRLLSLSEQE 180
           FMYEQ TDLPS +DWRERGAVN IKEQG CGSCWAFSSVAAVE IN+IKTN+LLSLSEQE
Sbjct: 121 FMYEQDTDLPSSVDWRERGAVNAIKEQGTCGSCWAFSSVAAVEAINKIKTNQLLSLSEQE 180

Query: 181 LLDCNIRNRGCNGGFMEIAFDFIKRNGGIATENSYPYHGARGLCRSSRISSPTVTIDGYE 240
           LLDCN RN+GCNGGFMEIAFDFIKRNGGIATENSYPYHG+RGLCRSSRISSP V IDGYE
Sbjct: 181 LLDCNYRNKGCNGGFMEIAFDFIKRNGGIATENSYPYHGSRGLCRSSRISSPIVKIDGYE 240

Query: 241 SVPENENALMQAVANQPVSVAIDA----------GVFDGYCGTELNHGVVAIGYGTTEEG 300
           SVPENE+ALMQAVANQPVSVAIDA          GVFDGYCGTELNHGVVAIGYGTTE+G
Sbjct: 241 SVPENEDALMQAVANQPVSVAIDAAGRDFQFYWQGVFDGYCGTELNHGVVAIGYGTTEDG 300

Query: 301 TDYWIVRNSWGVGWGEEGYVRMKRGVEQSEGLCGIVMEASYPIKY 334
           TDYWIVRNSWGVGWGE+GYVRMKRGVEQ EGLCGI MEASYPIK+
Sbjct: 301 TDYWIVRNSWGVGWGEDGYVRMKRGVEQPEGLCGIAMEASYPIKF 345

BLAST of Carg21840-RA vs. TrEMBL
Match: tr|A0A0A0LMU4|A0A0A0LMU4_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G349680 PE=3 SV=1)

HSP 1 Score: 617.8 bits (1592), Expect = 1.4e-173
Identity = 302/345 (87.54%), Postives = 319/345 (92.46%), Query Frame = 0

Query: 1   MAISKFLLVPFLLIVLVSGLGESFEFDEKELATEESLWRLYERWGNHHTISRGLKEKHKR 60
           MAI KFLLVP LLIVLVSGL ESFEFDEKELATEESLW+LYERWG HHTISR LKEKHKR
Sbjct: 1   MAIGKFLLVPLLLIVLVSGLAESFEFDEKELATEESLWQLYERWGKHHTISRNLKEKHKR 60

Query: 61  FNVFKENVNHVFTVNQMNKPYKLKLNKFADMSNSEFVSFYARSNISHYRKLHGKR--SGG 120
           F+VFKENVNHVFTVNQM+KPYKLKLNKFADMSN EFV+FYARSNISHYRKLH +R  +GG
Sbjct: 61  FSVFKENVNHVFTVNQMDKPYKLKLNKFADMSNYEFVNFYARSNISHYRKLHERRRGAGG 120

Query: 121 FMYEQATDLPSYIDWRERGAVNDIKEQGRCGSCWAFSSVAAVEGINQIKTNRLLSLSEQE 180
           FMYEQ TDLPS +DWRERGAVN +KEQGRCGSCWAFSSVAAVEGIN+IKTN+LLSLSEQE
Sbjct: 121 FMYEQDTDLPSSVDWRERGAVNAVKEQGRCGSCWAFSSVAAVEGINKIKTNQLLSLSEQE 180

Query: 181 LLDCNIRNRGCNGGFMEIAFDFIKRNGGIATENSYPYHGARGLCRSSRISSPTVTIDGYE 240
           LLDCN RN+GCNGGFMEIAFDFIKRNGGIATENSYPYHG+RGLCRSSRISSP V IDGYE
Sbjct: 181 LLDCNYRNKGCNGGFMEIAFDFIKRNGGIATENSYPYHGSRGLCRSSRISSPIVKIDGYE 240

Query: 241 SVPENENALMQAVANQPVSVAIDA----------GVFDGYCGTELNHGVVAIGYGTTEEG 300
           SVPENE+ALMQAVANQPVSVAIDA          GVFDGYCGTELNHGVVAIGYGTTE+G
Sbjct: 241 SVPENEDALMQAVANQPVSVAIDAAGRDFQFYSQGVFDGYCGTELNHGVVAIGYGTTEDG 300

Query: 301 TDYWIVRNSWGVGWGEEGYVRMKRGVEQSEGLCGIVMEASYPIKY 334
           TDYW+VRNSWGVGWGE+GYVRMKRGVEQ+EGLCGI MEASYPIKY
Sbjct: 301 TDYWLVRNSWGVGWGEDGYVRMKRGVEQAEGLCGIAMEASYPIKY 345

BLAST of Carg21840-RA vs. TrEMBL
Match: tr|A0A1U7ZK57|A0A1U7ZK57_NELNU (vignain-like OS=Nelumbo nucifera OX=4432 GN=LOC104591558 PE=3 SV=1)

HSP 1 Score: 448.4 bits (1152), Expect = 1.4e-122
Identity = 216/347 (62.25%), Postives = 267/347 (76.95%), Query Frame = 0

Query: 1   MAISKFLLVPFLLIVLVSGLGESFEFDEKELATEESLWRLYERWGNHHTISRGLKEKHKR 60
           MAI +  LV    +VLV  L ES EF EK+LA+EESLW+LYERW +HHT+SR L EKHKR
Sbjct: 1   MAIGRIALVALTTLVLVLSLAESLEFHEKDLASEESLWKLYERWRSHHTVSRDLSEKHKR 60

Query: 61  FNVFKENVNHVFTVNQMNKPYKLKLNKFADMSNSEFVSFYARSNISHYRKLHGKR--SGG 120
           FNVFKENVN++   N+ + PYKLKLNKFADM+N EF SFYA S ++H++ L G R  +GG
Sbjct: 61  FNVFKENVNYIHNFNKKDAPYKLKLNKFADMTNHEFRSFYASSRVNHHKMLRGSRRGTGG 120

Query: 121 FMYEQATDLPSYIDWRERGAVNDIKEQGRCGSCWAFSSVAAVEGINQIKTNRLLSLSEQE 180
           FMYE+  +LP  +DWRE+GAV  +K QG+CGSCWAFS+V AVEGIN+I+TN L++LSEQE
Sbjct: 121 FMYEKEDNLPPSVDWREKGAVTPVKNQGQCGSCWAFSTVVAVEGINKIRTNELVTLSEQE 180

Query: 181 LLDCN-IRNRGCNGGFMEIAFDFIKRNGGIATENSYPYHGARGLCRSSRISSPTVTIDGY 240
           L+DC+   N+GCNGG M+ AF+FIK  GG+ +E  YPY    G C +S+ +SP VTIDG+
Sbjct: 181 LVDCDKTENQGCNGGLMDYAFEFIKNTGGLTSEEIYPYRAEDGSCEASKKNSPMVTIDGH 240

Query: 241 ESVPEN-ENALMQAVANQPVSVAIDA----------GVFDGYCGTELNHGVVAIGYGTTE 300
           E+VP N E+ALM+AVANQPVSVAI+A          GVF G CGTEL+HGV  +GYGTT 
Sbjct: 241 ENVPANDEDALMKAVANQPVSVAIEAGGLDFQFYSEGVFTGSCGTELDHGVAIVGYGTTV 300

Query: 301 EGTDYWIVRNSWGVGWGEEGYVRMKRGVEQSEGLCGIVMEASYPIKY 334
           +GT YWIV+NSWG  WGE+GY+RM+RGV   EG+CGI MEASYPIKY
Sbjct: 301 DGTKYWIVKNSWGPEWGEKGYIRMQRGVSAKEGICGIAMEASYPIKY 347

BLAST of Carg21840-RA vs. TrEMBL
Match: tr|D7SME9|D7SME9_VITVI (Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VIT_01s0137g00330 PE=3 SV=1)

HSP 1 Score: 446.0 bits (1146), Expect = 7.2e-122
Identity = 221/346 (63.87%), Postives = 268/346 (77.46%), Query Frame = 0

Query: 1   MAISKFLLVPFLLIVLVSGLGESFEFDEKELATEESLWRLYERWGNHHTISRGLKEKHKR 60
           M + K +LV  L +VLV GL ESF+FDEK+LA+EESLW LYERW ++HT+SR L+EK+KR
Sbjct: 1   MKMEKVILVA-LSLVLVFGLAESFDFDEKDLASEESLWDLYERWRSYHTVSRDLEEKNKR 60

Query: 61  FNVFKENVNHVFTVNQMNKPYKLKLNKFADMSNSEFVSFYARSNISHYRKLHGKR--SGG 120
           FNVFKEN  HV  VNQM+KPYKLKLNKFADM+N EF S Y  S + HYR L G R  +GG
Sbjct: 61  FNVFKENTKHVHKVNQMDKPYKLKLNKFADMTNHEFRSSYGGSKVKHYRMLRGDRRGTGG 120

Query: 121 FMYEQATDLPSYIDWRERGAVNDIKEQGRCGSCWAFSSVAAVEGINQIKTNRLLSLSEQE 180
           FM+E+ T LP  +DWR++GAV  IK+QG+CGSCWAFS+V  VEGINQIKT  LLSLSEQ+
Sbjct: 121 FMHEKTTYLPPSVDWRKKGAVTGIKDQGKCGSCWAFSTVVGVEGINQIKTKELLSLSEQQ 180

Query: 181 LLDCN-IRNRGCNGGFMEIAFDFIKRNGGIATENSYPYHGARGLCRSSRISSPTVTIDGY 240
           L+DC+   + GCNGG ME AF+FIK+NGGI TEN+YPY      C   ++++P VTIDG+
Sbjct: 181 LIDCDRSDDHGCNGGLMESAFEFIKKNGGITTENNYPYKAKDERCDMLKMNAPVVTIDGH 240

Query: 241 ESVPEN-ENALMQAVANQPVSVAIDA----------GVFDGYCGTELNHGVVAIGYGTTE 300
           ESVP N E ALM+AVA+QPVSVAIDA          GVFDG CGTEL+HGV  +GYGTT 
Sbjct: 241 ESVPVNDERALMKAVAHQPVSVAIDAGGSDLQFYSEGVFDGECGTELDHGVAIVGYGTTL 300

Query: 301 EGTDYWIVRNSWGVGWGEEGYVRMKRGVEQSEGLCGIVMEASYPIK 333
           +GT YWIV+NSWG  WGE+GY+RM RG++ +EG CGI MEASYP+K
Sbjct: 301 DGTKYWIVKNSWGAEWGEKGYIRMARGIQAAEGQCGIAMEASYPVK 345

BLAST of Carg21840-RA vs. TrEMBL
Match: tr|A0A2P5DGE0|A0A2P5DGE0_PARAD (Cysteine Protease OS=Parasponia andersonii OX=3476 GN=PanCP10 PE=3 SV=1)

HSP 1 Score: 445.7 bits (1145), Expect = 9.4e-122
Identity = 221/346 (63.87%), Postives = 267/346 (77.17%), Query Frame = 0

Query: 1   MAISKFLLVPFLLIVLVSGLGESFEFDEKELATEESLWRLYERWGNHHTISRGLKEKHKR 60
           M + KFLLV  + + LV G+GESF+F E+EL +EESLW LY RW + HT+SR L EKHKR
Sbjct: 1   MQMKKFLLVA-ISLALVIGIGESFDFHEEELESEESLWDLYGRWRSSHTVSRSLDEKHKR 60

Query: 61  FNVFKENVNHVFTVNQMNKPYKLKLNKFADMSNSEFVSFYARSNISHYRKLHGKRSG--G 120
           FNVFKENV HV   N+ +KPYKLKLNKFADM+N EF S YA S I H+R   G R G   
Sbjct: 61  FNVFKENVKHVHNTNKKDKPYKLKLNKFADMTNHEFRSTYADSKIKHHRMFQGSRHGTVK 120

Query: 121 FMYEQATDLPSYIDWRERGAVNDIKEQGRCGSCWAFSSVAAVEGINQIKTNRLLSLSEQE 180
           FMYE+   +P+ +DWR++GAV+ +K+QG CGSCWAFS+VAAVEGINQI+TN+L+SLSEQE
Sbjct: 121 FMYEKVDSVPASVDWRKKGAVHAVKDQGNCGSCWAFSTVAAVEGINQIRTNKLVSLSEQE 180

Query: 181 LLDC-NIRNRGCNGGFMEIAFDFIKRNGGIATENSYPYHGARGLCRSSRISSPTVTIDGY 240
           L+DC N +N+GCNGG ME AF+FIK+ GG+ TE  YPY    G C +S+ +SP V+IDG+
Sbjct: 181 LVDCDNEQNQGCNGGLMEYAFEFIKKRGGLTTEIYYPYKAEDGSCDASKENSPAVSIDGH 240

Query: 241 ESVPEN-ENALMQAVANQPVSVAIDA----------GVFDGYCGTELNHGVVAIGYGTTE 300
           E+VPEN E+AL +AVANQPVSVAIDA          GVF+GYCGTELNHGV  +GYGTT 
Sbjct: 241 ENVPENDEDALQKAVANQPVSVAIDAGSSDFQFYSEGVFNGYCGTELNHGVAVVGYGTTL 300

Query: 301 EGTDYWIVRNSWGVGWGEEGYVRMKRGVEQSEGLCGIVMEASYPIK 333
           +GT YWIVRNSWG  WGE+GY+RM+R V   EGLCGI MEASYPIK
Sbjct: 301 DGTKYWIVRNSWGPEWGEKGYIRMQRHVSDKEGLCGIAMEASYPIK 345

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023545499.12.0e-18487.37vignain-like [Cucurbita pepo subsp. pepo][more]
XP_022996765.14.5e-18486.60vignain-like [Cucurbita maxima][more]
XP_004142960.12.1e-17387.54PREDICTED: vignain-like [Cucumis sativus] >KGN62334.1 hypothetical protein Csa_2... [more]
XP_008444390.12.1e-17387.83PREDICTED: vignain-like [Cucumis melo][more]
XP_023537428.12.2e-15980.35vignain-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT3G48350.12.7e-11459.08Cysteine proteinases superfamily protein[more]
AT3G48340.11.0e-11359.01Cysteine proteinases superfamily protein[more]
AT5G50260.13.1e-11055.85Cysteine proteinases superfamily protein[more]
AT5G43060.14.4e-8548.73Granulin repeat cysteine protease family protein[more]
AT1G47128.11.4e-8347.29Granulin repeat cysteine protease family protein[more]
Match NameE-valueIdentityDescription
sp|P12412|CYSEP_VIGMU3.1e-12061.27Vignain OS=Vigna mungo OX=3915 PE=1 SV=1[more]
sp|O65039|CYSEP_RICCO3.4e-11960.47Vignain OS=Ricinus communis OX=3988 GN=CYSEP PE=1 SV=1[more]
sp|P25803|CYSEP_PHAVU1.1e-11760.40Vignain OS=Phaseolus vulgaris OX=3885 PE=2 SV=2[more]
sp|Q9STL5|CEP3_ARATH4.8e-11359.08KDEL-tailed cysteine endopeptidase CEP3 OS=Arabidopsis thaliana OX=3702 GN=CEP3 ... [more]
sp|Q9STL4|CEP2_ARATH1.8e-11259.01KDEL-tailed cysteine endopeptidase CEP2 OS=Arabidopsis thaliana OX=3702 GN=CEP2 ... [more]
Match NameE-valueIdentityDescription
tr|A0A1S3BA70|A0A1S3BA70_CUCME1.4e-17387.83vignain-like OS=Cucumis melo OX=3656 GN=LOC103487731 PE=3 SV=1[more]
tr|A0A0A0LMU4|A0A0A0LMU4_CUCSA1.4e-17387.54Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G349680 PE=3 SV=1[more]
tr|A0A1U7ZK57|A0A1U7ZK57_NELNU1.4e-12262.25vignain-like OS=Nelumbo nucifera OX=4432 GN=LOC104591558 PE=3 SV=1[more]
tr|D7SME9|D7SME9_VITVI7.2e-12263.87Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VIT_01s0137g00330 PE=3 SV=... [more]
tr|A0A2P5DGE0|A0A2P5DGE0_PARAD9.4e-12263.87Cysteine Protease OS=Parasponia andersonii OX=3476 GN=PanCP10 PE=3 SV=1[more]
The following terms have been associated with this mRNA:
Vocabulary: Molecular Function
TermDefinition
GO:0008234cysteine-type peptidase activity
Vocabulary: Biological Process
TermDefinition
GO:0006508proteolysis
Vocabulary: INTERPRO
TermDefinition
IPR038765Papain_like_cys_pep_sf
IPR039417Peptidase_C1A_papain-like
IPR000169Pept_cys_AS
IPR025660Pept_his_AS
IPR025661Pept_asp_AS
IPR013128Peptidase_C1A
IPR013201Prot_inhib_I29
IPR000668Peptidase_C1A_C
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0005575 cellular_component
molecular_function GO:0008234 cysteine-type peptidase activity

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Carg21840Carg21840gene


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Carg21840-RACarg21840-RA-proteinpolypeptide


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg21840-RA.exon.1Carg21840-RA.exon.1exon
Carg21840-RA.exon.2Carg21840-RA.exon.2exon
Carg21840-RA.exon.3Carg21840-RA.exon.3exon
Carg21840-RA.exon.4Carg21840-RA.exon.4exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg21840-RA.CDS.1Carg21840-RA.CDS.1CDS
Carg21840-RA.CDS.2Carg21840-RA.CDS.2CDS
Carg21840-RA.CDS.3Carg21840-RA.CDS.3CDS
Carg21840-RA.CDS.4Carg21840-RA.CDS.4CDS


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000668Peptidase C1A, papain C-terminalPRINTSPR00705PAPAINcoord: 145..160
score: 64.05
coord: 275..285
score: 50.75
coord: 291..297
score: 73.06
IPR000668Peptidase C1A, papain C-terminalSMARTSM00645pept_c1coord: 127..331
e-value: 5.9E-100
score: 348.0
IPR000668Peptidase C1A, papain C-terminalPFAMPF00112Peptidase_C1coord: 127..331
e-value: 3.4E-68
score: 229.9
IPR013201Cathepsin propeptide inhibitor domain (I29)SMARTSM00848Inhibitor_I29_2coord: 41..96
e-value: 1.5E-10
score: 51.0
IPR013201Cathepsin propeptide inhibitor domain (I29)PFAMPF08246Inhibitor_I29coord: 41..96
e-value: 4.7E-11
score: 42.9
NoneNo IPR availableGENE3DG3DSA:3.90.70.10coord: 16..332
e-value: 1.1E-107
score: 362.4
NoneNo IPR availablePANTHERPTHR12411:SF346KDEL-TAILED CYSTEINE ENDOPEPTIDASE CEP2-RELATEDcoord: 5..332
IPR013128Peptidase C1APANTHERPTHR12411CYSTEINE PROTEASE FAMILY C1-RELATEDcoord: 5..332
IPR025661Cysteine peptidase, asparagine active sitePROSITEPS00640THIOL_PROTEASE_ASNcoord: 291..310
IPR025660Cysteine peptidase, histidine active sitePROSITEPS00639THIOL_PROTEASE_HIScoord: 273..283
IPR000169Cysteine peptidase, cysteine active sitePROSITEPS00139THIOL_PROTEASE_CYScoord: 145..156
IPR039417Papain-like cysteine endopeptidaseCDDcd02248Peptidase_C1Acoord: 128..330
e-value: 2.59763E-82
score: 250.234
IPR038765Papain-like cysteine peptidase superfamilySUPERFAMILYSSF54001Cysteine proteinasescoord: 33..331