Carg21184-RA (mRNA) Silver-seed gourd

NameCarg21184-RA
TypemRNA
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionProtein kinase domain
LocationCucurbita_argyrosperma_scaffold_207 : 450390 .. 455234 (-)
Sequence length2178
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTGGTTACCAAGATACAGCTTAGACAGAAAAGAGAGATTGAATGTGAATGGATTTGTGGCGGTGGCCATTGACAAAGACAAAGGCAGCCAAGGGGCTCTCAAATGGGCAATTGATCATTTGCTTCGCAGAGGCCAAACTGTTTTCCTCGTTCATGTCAAACTCAGATCCCTTTCTAATTCTTCCCTAAGTAAGTACTCTCTCTCTCTCTCTCAACTTTTTTATTTTTCATATCACAATTTTAGCTTCCATTAGGTTCGAAAGCTCGATCCGTTTCATTACAATTTTTTTTTAGATTCTCTATTCCTCGGAAATTTCATTTTTCATATATTTTTAAGAAGCAATGAGAGGTGTATACGGGTTGGATTGGGTTAATCTCATTGGTTTGTAAACATTAACCCCGAACTAAGTTTGTTTTTTGCAAAAGTTTAACTCAACCCAAATCAAAAATAAAAAACCTAACCAAACCCAACTTTTATAGTTTGAGTATGAATTTAGTAGTCCTGGTTGGTCGGATTATTGAGTCATTTTTAGGTTAGATATGAGAAAGAACATTTTATTATTAGGACGTATGTCTTAATGAGTCGAATATAAACTTTATTCATCAACGAATGATGAGAATATTTTGTTGAATTTAATCTTTCGAAAGTTTCTTTCTATTTCATTTCTTATGTATCAATTTCTTTAAGTTAAGCCTTTATCGTAAGTGAATATTAGGTTTACGTTCATCTAATACTCAAAACAGAACAAAACGTTTCATCGGCAACACAAGGTCGATTTGGTGTGGTGACTCACCTAACCCAAAATTATTTGTTTCTATAAAATATTTTACTTTGAATTCCCTTTGAGGAAAAAAAAAAAAACATAATTTTAGTGTATTTTTTGATTTTGCTCTCTAGAATGCTCATTTTCTATTAAATTTTATATTCTATATATAGATATATATATAATGAGGAAGCTCTTTTTGGATGCAGAGATGAAATCTGACCCTGAAGCTGGCGTTAGAGAATTATTCCTTCCTTTTCGTTGCTTTTGTACTCGAAAAGATGTAAGCTTTATTTATTTTATTTTATTTAATTGAAGATTCAATTTTTATCTATTATTTAGTATAATAATAATAAATTTTCTGAGTTTCAGATTCAATGCAAAGATGTTGTGTTGGAAGATACAGACATAGCTAAGGCCTTAACTGAGTATGCTGCAAATACAGCCATAGAGAACATTGTTGTGGGAGCTTCATCAAAATCTGGCTTTTTAAGGTATGTTTCTTTTCTTTTCTTTTCTTATTAGGTCAACATGTTTGAGAATCTTGATGATCCAAAAAACTCGACATGAATATTAAACTCGAACAATTGGATCGACTCGATAGGGTTTACCATGACCATGACGTTTTTCTCTTGGAGAATGTTTAATCATTTAGGCCGTTACCCATGACTCGATAGGCTTGGGCCGTTACAAATGACACCAGACTATGTGCCAACAAAGAGGTTGTTCCTCAAAGGGGGTGGACACGAGGCGGTGTGCCAGTAAGGACGCTGGGCCCGAAGGGGGGGTGGATTTGGTGGTGGTCCCACATCGATTGGAGAAACGAACAAGTGTCAGTGAGGACGCTGGGCCCTGAAGGGGGGTGGATTGTGATATCCCACATTGGTTGGGGAGGAGAACGAAACACCCTTTATAAGGGTGTGGAAACCTTGAGAGGAAGCCCAAAAAAGACAATATCTGCAACCCATATGCTTGGGCCGTTACACATATTAAGTGTTCGACTTGAAAAACTCGATCAATGCAACCCAAAAGCTATGAGTATCAAACTCGAACAGTTCGTTGAGTTGAAAAGGTCGTTTTTATGGCATATTATGTATGAAGAAAGCATTGAAGTTTCTTGTAGATATCATTAACTTGATACAAGCTTCTTATGAAGCAGATTCAAGGCAACTGATGTTCCTGGCAATGTCTCAAAAGGAGCACCAGATTTTTGCAATGTGTTTGTCATTTCTAAAGGCAAAATTCAATCGATGCGTTCGGCATCTCGTCCCGCTCCGGTCGTGAATCCTCTACAGTCGCAATTAGTGCAACAAAGCAGCACAAAATCCATCAGGTCCGACTACCAATTTCCTCAGACTACATCTGCAAGAGGTTAACTTCTTTCAAACCAGTTTCCTTTTACCTTATATGCTTGTTGGTCGTCCCAATATTCTCCATGAAACTAACGTTAAATTTGTTAGCAGCCTCTTTGGATAAACGTCCCTTTGTTCTGCCACGTAGATCGCAGGACGAGGCTGATTTCATGAGGTGAGAGCGACCCTATACATCGTTCAAATAGACGTTAAAACTTCAGTTGTTAACTTAGTAAGCTATGACTAAATTGTATGACGTTTTAATATATTAGCTATTAATATTGATCGAAGTAGAACAAAACACTTTTACTCCGATTTTGTGTTAAACTACTACTAAATCCACGAGAAGCGTAAACTAGTTATGTTCTTTGTGATGTCCCACGTCTGTTGGGGAGGAGAACAAAATACCTTTTATAAGGGTGTGGAAACCTTTCCCTAGCAGACGCGTTTTAAAAAACTTTGAGGGAAAGCCCGAAAGGGAAAGCCCAAAGAGGACAATATCTGCGAGTGGTGGGCCTGAGCTGTTACAAATGGTATCAGAGCTAGACACTGGGTGATGTGCCAGCGAGGACGTTGGACCACGAAGGGAGGGGGGTTTGGTGGGGTCCCACATCGATTGGAGAAAGGAACGAGTGCCAGTGAGGACCCTGGACCCTGAAAGGGGGTGGATTGTGATGTCCCACATCAGTTGGGGAGGAGAACGAAACACCCTTTATAAGGGTGTGGAAACCTCTCCCTAGAAGACGCGTTTTAAAAACCTTAAGGGAAAGCCCAAAGAGGACAATATCTGCTAGCGGTGGGCTTAGGCTGTTACATTCTTACTTTGTTAGGCATACTTTGTTAGGCATACTGATGAGCAAAATGTCGTTGAAATCAGGTCACCCTTCACAAGGAAAGGATACAATTACAAACCGTCGTATGGAGATTCGTCTTTTCAAGAATCAGATATATCGTACGTTAGCTCAGGAAGACCGAGCATTGATCGTATATTTCCTTCAGTTTATGACAGCCTAGATATGGGAGGAAGAGCCACCCCACCACGGATGTCGAGTAGTACAGATCTTGACTTGACTCAGAGCTTCGAGTCTCTACCGATGGGACGGTTATCGATGGATATGAACTTCCCGTCCGAGTTCTCTTCGATCTCGCAAGATAGTGATAGATTGTCAATCTCATCACAGTCTTTGGTAAGTTTTGAGCCACATTATTGCCTATGGTTCCATTCATTTTATGCAATAAAGCTTTGTTTTATATGAACTTTTATATGATGGTGCAGGAAGATGTGGAGGCTGAGATGCGTAGGCTCAAGCTAGAACTCAAGCAAACCATGGAAATGTACAGCACAGCCTGCAAGGAAGCACTTACAGCAAAACAAAAGGTAAGACGAACCGATCGATTAACACGGATCGATATCGAGACCAGCTTTCAATGGCTGTTTCATGATATCTATGTTTCGAGTTGCAGGCGGTCGAGCTGCAACGCTGGAAATTAGAAGAAGAACAAAGAATAGAAGAGGCGAGATTGGCTGGGGAAGCTGCATTGGCGCTGGCCGAACAGGAGAAGGCGAAGTCGAAAGCAGCCATCGAGGCAGCAGAGGCAGCTAGGAGGATAGCAGAATTGGAAGCACAGAAAAGAATGAAGGCTGAAATGAAAGCTCACAAAGAAGCTGAAGAAAAGAAAAGGGCTATTGATGCATTGTCACAAAGCGATATCAGGTATAGAAAGTATACGATCGAGGAGATCGAAGAGGCAACGGAATTCTTTGCTGAATCTCGTAAGATCGGAGAAGGCGGTTATGGTCCAGTTTACAAGTGTGATCTTGATCATACTCCTGTTGCCATTAAGGTTTTACGTCCCGATGCAACTCAAGGACGATCGCAGTTTAAACAAGAGGTACGTTACCGAACAACTTCCATGTTTTAGAACGTCTAAGAGGTCTCATAACTTTATCTAATATAACGATTCATAGGTAGAAGTACTTAGCTGTATACGCCATCCAAACATGGTGCTCCTCCTTGGAGCGTGCCCCGAGTACGGCTGCTTGGTCTACGAGTTCATGGCTAATGGGAGCTTAGAAGATCGACTTCTACAACGAGGAAACACGCCTCCACTTTCGTGGCAGCTCAGGTTTCAAATTGCAGGTGAAATTGGAACAGCATTGTTGTTCCTACACCAAACAAAGCCAGAGCCACTCGTGCATCGTGATCTCAAACCAGCTAATATTTTGCTCGATCGTAACTTTGTTAGTAAGATCAGTGACGTCGGCTTAGCTAGGCTCGTACCTCCATCGGTTGCGGATAACGTAACACAGTACCGAATGACATCGACTGCTGGAACGTTTTGCTACATTGATCCCGAGTATCAGCAGACAGGCATGCTTGGTGTGAAATCAGACATATACTCATTAGGCATCATGTTTCTCCAGCTGATAACTTCTCGGCCGCCAATGGGATTGACTCACCATGTTGAACGAGCGATCGAGAATGATATGTTTGCAAATATTCTCGATCCAGCCGTTACCGATTGGCCTGTCGAAGAAGCGTTGTCGTTCGCCAAGATGTCGCTCAAGTGTGCCGAGTTAAGACGTAAAGATAGGCCTGATCTTGGAAAGGAAATCCTTCCCGAGCTTAACAGGTTGAGAACTATTGCAGAAGATTATATACACCCAACACTGATGGGAGACATAAGCAGCCCCACTCGCAGCAGCAGCCAAGTTTCTCTACCGTTG

mRNA sequence

ATGTGGTTACCAAGATACAGCTTAGACAGAAAAGAGAGATTGAATGTGAATGGATTTGTGGCGGTGGCCATTGACAAAGACAAAGGCAGCCAAGGGGCTCTCAAATGGGCAATTGATCATTTGCTTCGCAGAGGCCAAACTGTTTTCCTCGTTCATGTCAAACTCAGATCCCTTTCTAATTCTTCCCTAAAGATGAAATCTGACCCTGAAGCTGGCGTTAGAGAATTATTCCTTCCTTTTCGTTGCTTTTGTACTCGAAAAGATATTCAATGCAAAGATGTTGTGTTGGAAGATACAGACATAGCTAAGGCCTTAACTGAGTATGCTGCAAATACAGCCATAGAGAACATTGTTGTGGGAGCTTCATCAAAATCTGGCTTTTTAAGATTCAAGGCAACTGATGTTCCTGGCAATGTCTCAAAAGGAGCACCAGATTTTTGCAATGTGTTTGTCATTTCTAAAGGCAAAATTCAATCGATGCGTTCGGCATCTCGTCCCGCTCCGGTCGTGAATCCTCTACAGTCGCAATTAGTGCAACAAAGCAGCACAAAATCCATCAGGTCCGACTACCAATTTCCTCAGACTACATCTGCAAGAGCCTCTTTGGATAAACGTCCCTTTGTTCTGCCACGTAGATCGCAGGACGAGGCTGATTTCATGAGCTCAGGAAGACCGAGCATTGATCGTATATTTCCTTCAGTTTATGACAGCCTAGATATGGGAGGAAGAGCCACCCCACCACGGATGTCGAGTAGTACAGATCTTGACTTGACTCAGAGCTTCGAGTCTCTACCGATGGGACGGTTATCGATGGATATGAACTTCCCGTCCGAGTTCTCTTCGATCTCGCAAGATAGTGATAGATTGTCAATCTCATCACAGTCTTTGGAAGATGTGGAGGCTGAGATGCGTAGGCTCAAGCTAGAACTCAAGCAAACCATGGAAATGTACAGCACAGCCTGCAAGGAAGCACTTACAGCAAAACAAAAGGCGGTCGAGCTGCAACGCTGGAAATTAGAAGAAGAACAAAGAATAGAAGAGGCGAGATTGGCTGGGGAAGCTGCATTGGCGCTGGCCGAACAGGAGAAGGCGAAGTCGAAAGCAGCCATCGAGGCAGCAGAGGCAGCTAGGAGGATAGCAGAATTGGAAGCACAGAAAAGAATGAAGGCTGAAATGAAAGCTCACAAAGAAGCTGAAGAAAAGAAAAGGGCTATTGATGCATTGTCACAAAGCGATATCAGGTATAGAAAGTATACGATCGAGGAGATCGAAGAGGCAACGGAATTCTTTGCTGAATCTCGTAAGATCGGAGAAGGCGGTTATGGTCCAGTTTACAAGTGTGATCTTGATCATACTCCTGTTGCCATTAAGGTTTTACGTCCCGATGCAACTCAAGGACGATCGCAGTTTAAACAAGAGGTAGAAGTACTTAGCTGTATACGCCATCCAAACATGGTGCTCCTCCTTGGAGCGTGCCCCGAGTACGGCTGCTTGGTCTACGAGTTCATGGCTAATGGGAGCTTAGAAGATCGACTTCTACAACGAGGAAACACGCCTCCACTTTCGTGGCAGCTCAGGTTTCAAATTGCAGGTGAAATTGGAACAGCATTGTTGTTCCTACACCAAACAAAGCCAGAGCCACTCGTGCATCGTGATCTCAAACCAGCTAATATTTTGCTCGATCGTAACTTTGTTAGTAAGATCAGTGACGTCGGCTTAGCTAGGCTCGTACCTCCATCGGTTGCGGATAACGTAACACAGTACCGAATGACATCGACTGCTGGAACGTTTTGCTACATTGATCCCGAGTATCAGCAGACAGGCATGCTTGGTGTGAAATCAGACATATACTCATTAGGCATCATGTTTCTCCAGCTGATAACTTCTCGGCCGCCAATGGGATTGACTCACCATGTTGAACGAGCGATCGAGAATGATATGTTTGCAAATATTCTCGATCCAGCCGTTACCGATTGGCCTGTCGAAGAAGCGTTGTCGTTCGCCAAGATGTCGCTCAAGTGTGCCGAGTTAAGACGTAAAGATAGGCCTGATCTTGGAAAGGAAATCCTTCCCGAGCTTAACAGGTTGAGAACTATTGCAGAAGATTATATACACCCAACACTGATGGGAGACATAAGCAGCCCCACTCGCAGCAGCAGCCAAGTTTCTCTACCGTTG

Coding sequence (CDS)

ATGTGGTTACCAAGATACAGCTTAGACAGAAAAGAGAGATTGAATGTGAATGGATTTGTGGCGGTGGCCATTGACAAAGACAAAGGCAGCCAAGGGGCTCTCAAATGGGCAATTGATCATTTGCTTCGCAGAGGCCAAACTGTTTTCCTCGTTCATGTCAAACTCAGATCCCTTTCTAATTCTTCCCTAAAGATGAAATCTGACCCTGAAGCTGGCGTTAGAGAATTATTCCTTCCTTTTCGTTGCTTTTGTACTCGAAAAGATATTCAATGCAAAGATGTTGTGTTGGAAGATACAGACATAGCTAAGGCCTTAACTGAGTATGCTGCAAATACAGCCATAGAGAACATTGTTGTGGGAGCTTCATCAAAATCTGGCTTTTTAAGATTCAAGGCAACTGATGTTCCTGGCAATGTCTCAAAAGGAGCACCAGATTTTTGCAATGTGTTTGTCATTTCTAAAGGCAAAATTCAATCGATGCGTTCGGCATCTCGTCCCGCTCCGGTCGTGAATCCTCTACAGTCGCAATTAGTGCAACAAAGCAGCACAAAATCCATCAGGTCCGACTACCAATTTCCTCAGACTACATCTGCAAGAGCCTCTTTGGATAAACGTCCCTTTGTTCTGCCACGTAGATCGCAGGACGAGGCTGATTTCATGAGCTCAGGAAGACCGAGCATTGATCGTATATTTCCTTCAGTTTATGACAGCCTAGATATGGGAGGAAGAGCCACCCCACCACGGATGTCGAGTAGTACAGATCTTGACTTGACTCAGAGCTTCGAGTCTCTACCGATGGGACGGTTATCGATGGATATGAACTTCCCGTCCGAGTTCTCTTCGATCTCGCAAGATAGTGATAGATTGTCAATCTCATCACAGTCTTTGGAAGATGTGGAGGCTGAGATGCGTAGGCTCAAGCTAGAACTCAAGCAAACCATGGAAATGTACAGCACAGCCTGCAAGGAAGCACTTACAGCAAAACAAAAGGCGGTCGAGCTGCAACGCTGGAAATTAGAAGAAGAACAAAGAATAGAAGAGGCGAGATTGGCTGGGGAAGCTGCATTGGCGCTGGCCGAACAGGAGAAGGCGAAGTCGAAAGCAGCCATCGAGGCAGCAGAGGCAGCTAGGAGGATAGCAGAATTGGAAGCACAGAAAAGAATGAAGGCTGAAATGAAAGCTCACAAAGAAGCTGAAGAAAAGAAAAGGGCTATTGATGCATTGTCACAAAGCGATATCAGGTATAGAAAGTATACGATCGAGGAGATCGAAGAGGCAACGGAATTCTTTGCTGAATCTCGTAAGATCGGAGAAGGCGGTTATGGTCCAGTTTACAAGTGTGATCTTGATCATACTCCTGTTGCCATTAAGGTTTTACGTCCCGATGCAACTCAAGGACGATCGCAGTTTAAACAAGAGGTAGAAGTACTTAGCTGTATACGCCATCCAAACATGGTGCTCCTCCTTGGAGCGTGCCCCGAGTACGGCTGCTTGGTCTACGAGTTCATGGCTAATGGGAGCTTAGAAGATCGACTTCTACAACGAGGAAACACGCCTCCACTTTCGTGGCAGCTCAGGTTTCAAATTGCAGGTGAAATTGGAACAGCATTGTTGTTCCTACACCAAACAAAGCCAGAGCCACTCGTGCATCGTGATCTCAAACCAGCTAATATTTTGCTCGATCGTAACTTTGTTAGTAAGATCAGTGACGTCGGCTTAGCTAGGCTCGTACCTCCATCGGTTGCGGATAACGTAACACAGTACCGAATGACATCGACTGCTGGAACGTTTTGCTACATTGATCCCGAGTATCAGCAGACAGGCATGCTTGGTGTGAAATCAGACATATACTCATTAGGCATCATGTTTCTCCAGCTGATAACTTCTCGGCCGCCAATGGGATTGACTCACCATGTTGAACGAGCGATCGAGAATGATATGTTTGCAAATATTCTCGATCCAGCCGTTACCGATTGGCCTGTCGAAGAAGCGTTGTCGTTCGCCAAGATGTCGCTCAAGTGTGCCGAGTTAAGACGTAAAGATAGGCCTGATCTTGGAAAGGAAATCCTTCCCGAGCTTAACAGGTTGAGAACTATTGCAGAAGATTATATACACCCAACACTGATGGGAGACATAAGCAGCCCCACTCGCAGCAGCAGCCAAGTTTCTCTACCGTTG

Protein sequence

MWLPRYSLDRKERLNVNGFVAVAIDKDKGSQGALKWAIDHLLRRGQTVFLVHVKLRSLSNSSLKMKSDPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIVVGASSKSGFLRFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVNPLQSQLVQQSSTKSIRSDYQFPQTTSARASLDKRPFVLPRRSQDEADFMSSGRPSIDRIFPSVYDSLDMGGRATPPRMSSSTDLDLTQSFESLPMGRLSMDMNFPSEFSSISQDSDRLSISSQSLEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKAVELQRWKLEEEQRIEEARLAGEAALALAEQEKAKSKAAIEAAEAARRIAELEAQKRMKAEMKAHKEAEEKKRAIDALSQSDIRYRKYTIEEIEEATEFFAESRKIGEGGYGPVYKCDLDHTPVAIKVLRPDATQGRSQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDRLLQRGNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDVGLARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRPPMGLTHHVERAIENDMFANILDPAVTDWPVEEALSFAKMSLKCAELRRKDRPDLGKEILPELNRLRTIAEDYIHPTLMGDISSPTRSSSQVSLPL
BLAST of Carg21184-RA vs. NCBI nr
Match: XP_022941502.1 (U-box domain-containing protein 52-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 1315.1 bits (3402), Expect = 0.0e+00
Identity = 721/754 (95.62%), Postives = 723/754 (95.89%), Query Frame = 0

Query: 1   MWLPRYSLDRKERLNVNGFVAVAIDKDKGSQGALKWAIDHLLRRGQTVFLVHVKLRSLSN 60
           MWLPRYSLDRKERLNVNGFVAVAIDKDKGSQGALKWAIDHLLRRGQTVFLVHVKLRS SN
Sbjct: 1   MWLPRYSLDRKERLNVNGFVAVAIDKDKGSQGALKWAIDHLLRRGQTVFLVHVKLRSPSN 60

Query: 61  SSLKMKSDPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIVVG 120
           SSLKMKSDPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIVVG
Sbjct: 61  SSLKMKSDPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIVVG 120

Query: 121 ASSKSGFLRFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVNPLQSQLVQQ 180
           ASSKSGFLRFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVNPL+SQLVQQ
Sbjct: 121 ASSKSGFLRFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVNPLRSQLVQQ 180

Query: 181 SSTKSIRSDYQFPQTTSARASLDKRPFVLPRRSQDEADFM-------------------- 240
           SSTKSI SDYQFPQTTSARASLDKRPFVLPRRSQDEADFM                    
Sbjct: 181 SSTKSISSDYQFPQTTSARASLDKRPFVLPRRSQDEADFMRSPFTRKGYNYKPSYGDSSF 240

Query: 241 --------SSGRPSIDRIFPSVYDSLDMGGRATPPRMSSSTDLDLTQSFESLPMGRLSMD 300
                   SSGRPSIDRIFPSVYDSLDMGGRATPPRMSSSTDLDLTQSFESLPMGRLSMD
Sbjct: 241 QESDISYVSSGRPSIDRIFPSVYDSLDMGGRATPPRMSSSTDLDLTQSFESLPMGRLSMD 300

Query: 301 MNFPSEFSSISQDSDRLSISSQSLEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKAV 360
           MNFPSEFSSISQDSDRLSISSQSLEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKAV
Sbjct: 301 MNFPSEFSSISQDSDRLSISSQSLEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKAV 360

Query: 361 ELQRWKLEEEQRIEEARLAGEAALALAEQEKAKXXXXXXXXXXXXXXXXXXXXXXXXXXX 420
           ELQRWKLEEEQRIEEARLAGEAALALAEQEKAKXXXXXXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 361 ELQRWKLEEEQRIEEARLAGEAALALAEQEKAKXXXXXXXXXXXXXXXXXXXXXXXXXXX 420

Query: 421 XXXXXXXXKKRAIDALSQSDIRYRKYTIEEIEEATEFFAESRKIGEGGYGPVYKCDLDHT 480
           XXXXXXXXKKRAIDALSQ+DIRYRKYTIEEIEEATEFFAESRKIGEGGYGPVYKCDLDHT
Sbjct: 421 XXXXXXXXKKRAIDALSQNDIRYRKYTIEEIEEATEFFAESRKIGEGGYGPVYKCDLDHT 480

Query: 481 PVAIKVLRPDATQGRSQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDRL 540
           PVAIKVLRPDATQGRSQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDRL
Sbjct: 481 PVAIKVLRPDATQGRSQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDRL 540

Query: 541 LQRGNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDVG 600
           LQRGNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDVG
Sbjct: 541 LQRGNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDVG 600

Query: 601 LARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRPP 660
           LARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRPP
Sbjct: 601 LARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRPP 660

Query: 661 MGLTHHVERAIENDMFANILDPAVTDWPVEEALSFAKMSLKCAELRRKDRPDLGKEILPE 720
           MGLTHHVERAIENDMFANILDPAVTDWPVEEALSFAKMSLKCAELRRKDRPDLGKEILPE
Sbjct: 661 MGLTHHVERAIENDMFANILDPAVTDWPVEEALSFAKMSLKCAELRRKDRPDLGKEILPE 720

Query: 721 LNRLRTIAEDYIHPTLMGDISSPTRSSSQVSLPL 727
           LNRLRTIAEDYIHPTLMGDI SPTRSSSQVSLPL
Sbjct: 721 LNRLRTIAEDYIHPTLMGDI-SPTRSSSQVSLPL 753

BLAST of Carg21184-RA vs. NCBI nr
Match: XP_022941501.1 (U-box domain-containing protein 52-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 1310.4 bits (3390), Expect = 0.0e+00
Identity = 721/755 (95.50%), Postives = 723/755 (95.76%), Query Frame = 0

Query: 1   MWLPRYSLDRKERLNVNGFVAVAIDKDKGSQGALKWAIDHLLRRGQTVFLVHVKLRSLSN 60
           MWLPRYSLDRKERLNVNGFVAVAIDKDKGSQGALKWAIDHLLRRGQTVFLVHVKLRS SN
Sbjct: 1   MWLPRYSLDRKERLNVNGFVAVAIDKDKGSQGALKWAIDHLLRRGQTVFLVHVKLRSPSN 60

Query: 61  SSLKMKSDPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIVVG 120
           SSLKMKSDPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIVVG
Sbjct: 61  SSLKMKSDPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIVVG 120

Query: 121 ASSKSGFL-RFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVNPLQSQLVQ 180
           ASSKSGFL RFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVNPL+SQLVQ
Sbjct: 121 ASSKSGFLSRFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVNPLRSQLVQ 180

Query: 181 QSSTKSIRSDYQFPQTTSARASLDKRPFVLPRRSQDEADFM------------------- 240
           QSSTKSI SDYQFPQTTSARASLDKRPFVLPRRSQDEADFM                   
Sbjct: 181 QSSTKSISSDYQFPQTTSARASLDKRPFVLPRRSQDEADFMRSPFTRKGYNYKPSYGDSS 240

Query: 241 ---------SSGRPSIDRIFPSVYDSLDMGGRATPPRMSSSTDLDLTQSFESLPMGRLSM 300
                    SSGRPSIDRIFPSVYDSLDMGGRATPPRMSSSTDLDLTQSFESLPMGRLSM
Sbjct: 241 FQESDISYVSSGRPSIDRIFPSVYDSLDMGGRATPPRMSSSTDLDLTQSFESLPMGRLSM 300

Query: 301 DMNFPSEFSSISQDSDRLSISSQSLEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKA 360
           DMNFPSEFSSISQDSDRLSISSQSLEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKA
Sbjct: 301 DMNFPSEFSSISQDSDRLSISSQSLEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKA 360

Query: 361 VELQRWKLEEEQRIEEARLAGEAALALAEQEKAKXXXXXXXXXXXXXXXXXXXXXXXXXX 420
           VELQRWKLEEEQRIEEARLAGEAALALAEQEKAKXXXXXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 361 VELQRWKLEEEQRIEEARLAGEAALALAEQEKAKXXXXXXXXXXXXXXXXXXXXXXXXXX 420

Query: 421 XXXXXXXXXKKRAIDALSQSDIRYRKYTIEEIEEATEFFAESRKIGEGGYGPVYKCDLDH 480
           XXXXXXXXXKKRAIDALSQ+DIRYRKYTIEEIEEATEFFAESRKIGEGGYGPVYKCDLDH
Sbjct: 421 XXXXXXXXXKKRAIDALSQNDIRYRKYTIEEIEEATEFFAESRKIGEGGYGPVYKCDLDH 480

Query: 481 TPVAIKVLRPDATQGRSQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDR 540
           TPVAIKVLRPDATQGRSQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDR
Sbjct: 481 TPVAIKVLRPDATQGRSQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDR 540

Query: 541 LLQRGNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDV 600
           LLQRGNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDV
Sbjct: 541 LLQRGNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDV 600

Query: 601 GLARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRP 660
           GLARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRP
Sbjct: 601 GLARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRP 660

Query: 661 PMGLTHHVERAIENDMFANILDPAVTDWPVEEALSFAKMSLKCAELRRKDRPDLGKEILP 720
           PMGLTHHVERAIENDMFANILDPAVTDWPVEEALSFAKMSLKCAELRRKDRPDLGKEILP
Sbjct: 661 PMGLTHHVERAIENDMFANILDPAVTDWPVEEALSFAKMSLKCAELRRKDRPDLGKEILP 720

Query: 721 ELNRLRTIAEDYIHPTLMGDISSPTRSSSQVSLPL 727
           ELNRLRTIAEDYIHPTLMGDI SPTRSSSQVSLPL
Sbjct: 721 ELNRLRTIAEDYIHPTLMGDI-SPTRSSSQVSLPL 754

BLAST of Carg21184-RA vs. NCBI nr
Match: XP_023524077.1 (U-box domain-containing protein 52-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1306.2 bits (3379), Expect = 0.0e+00
Identity = 700/754 (92.84%), Postives = 706/754 (93.63%), Query Frame = 0

Query: 1   MWLPRYSLDRKERLNVNGFVAVAIDKDKGSQGALKWAIDHLLRRGQTVFLVHVKLRSLSN 60
           MWLPRYSLDRKERLNVNGFVAVAIDKDKGSQGALKWAIDHLLRRGQTVFLVHVKLRS SN
Sbjct: 1   MWLPRYSLDRKERLNVNGFVAVAIDKDKGSQGALKWAIDHLLRRGQTVFLVHVKLRSPSN 60

Query: 61  SSLKMKSDPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIVVG 120
           SSLKMKSDPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIVVG
Sbjct: 61  SSLKMKSDPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIVVG 120

Query: 121 ASSKSGFLRFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVNPLQSQLVQQ 180
           ASSKSGFLRFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVNPL+SQLVQQ
Sbjct: 121 ASSKSGFLRFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVNPLRSQLVQQ 180

Query: 181 SSTKSIRSDYQFPQTTSARASLDKRPFVLPRRSQDEADFM-------------------- 240
           SSTKSIRSDYQFPQTTSARASLDKRPFVLPR SQDEADFM                    
Sbjct: 181 SSTKSIRSDYQFPQTTSARASLDKRPFVLPRGSQDEADFMRSPFTRKGYNYKPSYGDSSF 240

Query: 241 --------SSGRPSIDRIFPSVYDSLDMGGRATPPRMSSSTDLDLTQSFESLPMGRLSMD 300
                   SSGRPSIDRIFPSVYDSLDMGGRATPPRMSSSTDLDLTQSFESLPMGRLSMD
Sbjct: 241 QESDISYVSSGRPSIDRIFPSVYDSLDMGGRATPPRMSSSTDLDLTQSFESLPMGRLSMD 300

Query: 301 MNFPSEFSSISQDSDRLSISSQSLEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKAV 360
           MNFPSEFSSISQDSDRLSISSQSLEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKAV
Sbjct: 301 MNFPSEFSSISQDSDRLSISSQSLEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKAV 360

Query: 361 ELQRWKLEEEQRIEEARLAGEAALALAEQEKAKXXXXXXXXXXXXXXXXXXXXXXXXXXX 420
           ELQRWKLEEEQRIEEARLAGEAALALA+QEK K              XXXXXXXXXXXXX
Sbjct: 361 ELQRWKLEEEQRIEEARLAGEAALALAKQEKTKSKAAIEAAEAARRIXXXXXXXXXXXXX 420

Query: 421 XXXXXXXXKKRAIDALSQSDIRYRKYTIEEIEEATEFFAESRKIGEGGYGPVYKCDLDHT 480
           XXXXXXX KKRAIDALSQSDIRYRKYTIEEIEEATEFFAESRKIGEGGYGPVY+CDLDHT
Sbjct: 421 XXXXXXXEKKRAIDALSQSDIRYRKYTIEEIEEATEFFAESRKIGEGGYGPVYRCDLDHT 480

Query: 481 PVAIKVLRPDATQGRSQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDRL 540
           PVAIKVLRPDATQGRSQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDRL
Sbjct: 481 PVAIKVLRPDATQGRSQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDRL 540

Query: 541 LQRGNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDVG 600
           LQRGNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDVG
Sbjct: 541 LQRGNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDVG 600

Query: 601 LARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRPP 660
           LARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRPP
Sbjct: 601 LARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRPP 660

Query: 661 MGLTHHVERAIENDMFANILDPAVTDWPVEEALSFAKMSLKCAELRRKDRPDLGKEILPE 720
           MGLTHHVE+AIENDMFANILDPA+TDWP+EEALSFAKMSLKCAELRRKDRPDLGKEILPE
Sbjct: 661 MGLTHHVEQAIENDMFANILDPAITDWPIEEALSFAKMSLKCAELRRKDRPDLGKEILPE 720

Query: 721 LNRLRTIAEDYIHPTLMGDISSPTRSSSQVSLPL 727
           LNRLRTIAEDYIHPTLMGDI SPTRSSSQVSL L
Sbjct: 721 LNRLRTIAEDYIHPTLMGDI-SPTRSSSQVSLQL 753

BLAST of Carg21184-RA vs. NCBI nr
Match: XP_023524076.1 (U-box domain-containing protein 52-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1301.6 bits (3367), Expect = 0.0e+00
Identity = 700/755 (92.72%), Postives = 706/755 (93.51%), Query Frame = 0

Query: 1   MWLPRYSLDRKERLNVNGFVAVAIDKDKGSQGALKWAIDHLLRRGQTVFLVHVKLRSLSN 60
           MWLPRYSLDRKERLNVNGFVAVAIDKDKGSQGALKWAIDHLLRRGQTVFLVHVKLRS SN
Sbjct: 1   MWLPRYSLDRKERLNVNGFVAVAIDKDKGSQGALKWAIDHLLRRGQTVFLVHVKLRSPSN 60

Query: 61  SSLKMKSDPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIVVG 120
           SSLKMKSDPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIVVG
Sbjct: 61  SSLKMKSDPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIVVG 120

Query: 121 ASSKSGFL-RFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVNPLQSQLVQ 180
           ASSKSGFL RFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVNPL+SQLVQ
Sbjct: 121 ASSKSGFLSRFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVNPLRSQLVQ 180

Query: 181 QSSTKSIRSDYQFPQTTSARASLDKRPFVLPRRSQDEADFM------------------- 240
           QSSTKSIRSDYQFPQTTSARASLDKRPFVLPR SQDEADFM                   
Sbjct: 181 QSSTKSIRSDYQFPQTTSARASLDKRPFVLPRGSQDEADFMRSPFTRKGYNYKPSYGDSS 240

Query: 241 ---------SSGRPSIDRIFPSVYDSLDMGGRATPPRMSSSTDLDLTQSFESLPMGRLSM 300
                    SSGRPSIDRIFPSVYDSLDMGGRATPPRMSSSTDLDLTQSFESLPMGRLSM
Sbjct: 241 FQESDISYVSSGRPSIDRIFPSVYDSLDMGGRATPPRMSSSTDLDLTQSFESLPMGRLSM 300

Query: 301 DMNFPSEFSSISQDSDRLSISSQSLEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKA 360
           DMNFPSEFSSISQDSDRLSISSQSLEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKA
Sbjct: 301 DMNFPSEFSSISQDSDRLSISSQSLEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKA 360

Query: 361 VELQRWKLEEEQRIEEARLAGEAALALAEQEKAKXXXXXXXXXXXXXXXXXXXXXXXXXX 420
           VELQRWKLEEEQRIEEARLAGEAALALA+QEK K              XXXXXXXXXXXX
Sbjct: 361 VELQRWKLEEEQRIEEARLAGEAALALAKQEKTKSKAAIEAAEAARRIXXXXXXXXXXXX 420

Query: 421 XXXXXXXXXKKRAIDALSQSDIRYRKYTIEEIEEATEFFAESRKIGEGGYGPVYKCDLDH 480
           XXXXXXXX KKRAIDALSQSDIRYRKYTIEEIEEATEFFAESRKIGEGGYGPVY+CDLDH
Sbjct: 421 XXXXXXXXEKKRAIDALSQSDIRYRKYTIEEIEEATEFFAESRKIGEGGYGPVYRCDLDH 480

Query: 481 TPVAIKVLRPDATQGRSQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDR 540
           TPVAIKVLRPDATQGRSQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDR
Sbjct: 481 TPVAIKVLRPDATQGRSQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDR 540

Query: 541 LLQRGNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDV 600
           LLQRGNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDV
Sbjct: 541 LLQRGNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDV 600

Query: 601 GLARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRP 660
           GLARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRP
Sbjct: 601 GLARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRP 660

Query: 661 PMGLTHHVERAIENDMFANILDPAVTDWPVEEALSFAKMSLKCAELRRKDRPDLGKEILP 720
           PMGLTHHVE+AIENDMFANILDPA+TDWP+EEALSFAKMSLKCAELRRKDRPDLGKEILP
Sbjct: 661 PMGLTHHVEQAIENDMFANILDPAITDWPIEEALSFAKMSLKCAELRRKDRPDLGKEILP 720

Query: 721 ELNRLRTIAEDYIHPTLMGDISSPTRSSSQVSLPL 727
           ELNRLRTIAEDYIHPTLMGDI SPTRSSSQVSL L
Sbjct: 721 ELNRLRTIAEDYIHPTLMGDI-SPTRSSSQVSLQL 754

BLAST of Carg21184-RA vs. NCBI nr
Match: XP_022982091.1 (U-box domain-containing protein 35-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 1295.0 bits (3350), Expect = 0.0e+00
Identity = 709/754 (94.03%), Postives = 717/754 (95.09%), Query Frame = 0

Query: 1   MWLPRYSLDRKERLNVNGFVAVAIDKDKGSQGALKWAIDHLLRRGQTVFLVHVKLRSLSN 60
           MWLPRYSLDRKERLNVNGFVAVAIDKDKGSQGALKWAIDHLLRRGQTVFLVHVKLRS SN
Sbjct: 1   MWLPRYSLDRKERLNVNGFVAVAIDKDKGSQGALKWAIDHLLRRGQTVFLVHVKLRSPSN 60

Query: 61  SSLKMKSDPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIVVG 120
           SSLKMK DPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIVVG
Sbjct: 61  SSLKMKCDPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIVVG 120

Query: 121 ASSKSGFLRFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVNPLQSQLVQQ 180
           ASSKSGFLRFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVNPL+SQLVQQ
Sbjct: 121 ASSKSGFLRFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVNPLRSQLVQQ 180

Query: 181 SSTKSIRSDYQFPQTTSARASLDKRPFVLPRRSQDEADFM-------------------- 240
           SSTKSIRSDYQFPQTTSAR SLDKRP  LPRRSQDE DFM                    
Sbjct: 181 SSTKSIRSDYQFPQTTSARDSLDKRPIDLPRRSQDETDFMRSPFTRKGYNYKPSYGDSSF 240

Query: 241 --------SSGRPSIDRIFPSVYDSLDMGGRATPPRMSSSTDLDLTQSFESLPMGRLSMD 300
                   SSGRPSIDRIFPSVYDSLDMGGRATPPRMSSST+LDLT+SFESLPMGRLSMD
Sbjct: 241 QESDISYVSSGRPSIDRIFPSVYDSLDMGGRATPPRMSSSTNLDLTRSFESLPMGRLSMD 300

Query: 301 MNFPSEFSSISQDSDRLSISSQSLEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKAV 360
           MNFPSEFSSISQDSDRLSISSQSLEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKAV
Sbjct: 301 MNFPSEFSSISQDSDRLSISSQSLEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKAV 360

Query: 361 ELQRWKLEEEQRIEEARLAGEAALALAEQEKAKXXXXXXXXXXXXXXXXXXXXXXXXXXX 420
           ELQRWKLEEEQRIEEARLAGEAALALAEQEKAKXXXXXXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 361 ELQRWKLEEEQRIEEARLAGEAALALAEQEKAKXXXXXXXXXXXXXXXXXXXXXXXXXXX 420

Query: 421 XXXXXXXXKKRAIDALSQSDIRYRKYTIEEIEEATEFFAESRKIGEGGYGPVYKCDLDHT 480
           XXXXXXXXKKRAIDALSQSDIRYRKYTIEEIEEATEFFAESRKIGEGGYGPVY CDLDHT
Sbjct: 421 XXXXXXXXKKRAIDALSQSDIRYRKYTIEEIEEATEFFAESRKIGEGGYGPVYNCDLDHT 480

Query: 481 PVAIKVLRPDATQGRSQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDRL 540
           PVAIKVLRPDATQGR QFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDRL
Sbjct: 481 PVAIKVLRPDATQGRLQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDRL 540

Query: 541 LQRGNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDVG 600
           L+RGNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDVG
Sbjct: 541 LRRGNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDVG 600

Query: 601 LARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRPP 660
           LARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRPP
Sbjct: 601 LARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRPP 660

Query: 661 MGLTHHVERAIENDMFANILDPAVTDWPVEEALSFAKMSLKCAELRRKDRPDLGKEILPE 720
           MGLTHHVERAIENDMFANILDPAVTDWPVEEALSFAK+SLKCAELRRKDRPDLGKE+LPE
Sbjct: 661 MGLTHHVERAIENDMFANILDPAVTDWPVEEALSFAKLSLKCAELRRKDRPDLGKEVLPE 720

Query: 721 LNRLRTIAEDYIHPTLMGDISSPTRSSSQVSLPL 727
           LNRLRTIAEDY+HPTLMGDI SPTRSSSQVS+PL
Sbjct: 721 LNRLRTIAEDYMHPTLMGDI-SPTRSSSQVSIPL 753

BLAST of Carg21184-RA vs. TAIR10
Match: AT4G31230.1 (Protein kinase protein with adenine nucleotide alpha hydrolases-like domain)

HSP 1 Score: 836.3 bits (2159), Expect = 1.5e-242
Identity = 465/762 (61.02%), Postives = 560/762 (73.49%), Query Frame = 0

Query: 9   DRKERLNVNGFVAVAIDKDKGSQGALKWAIDHLLRRGQTVFLVHVKLRSLS---NSSLKM 68
           DR     V+G VAVAID+DK SQGALKWA+D+LL++GQTV LVHVK R+ S   N S+  
Sbjct: 7   DRDGGFGVDGLVAVAIDRDKNSQGALKWAVDNLLQKGQTVVLVHVKPRASSLSTNPSINS 66

Query: 69  KS---------------DPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAA 128
            S               +PE   ++LFLPFRC C+RKDIQCKDV+LE++D+A+AL EYA 
Sbjct: 67  NSSKTSQINGDSSLVCGEPEGSYKQLFLPFRCLCSRKDIQCKDVLLEESDVARALVEYAN 126

Query: 129 NTAIENIVVGASSKSGFLRF-KATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPV 188
              IE +VVG+SSK GFLRF K  D+PG ++K APDFC V+ I+KGK+ + ++ASR AP 
Sbjct: 127 QVMIEVLVVGSSSKGGFLRFNKPIDIPGAITKTAPDFCTVYAITKGKLSTKKTASRAAPS 186

Query: 189 VNPLQSQLVQQ---------SSTKSIRSDYQF----------PQTTSARASLDKRPFVLP 248
           V+PL+ QL Q          S+T + R++ Q            Q+ S R   +KR   L 
Sbjct: 187 VSPLRIQLQQNGLKPHPPLPSATTNTRAERQSFESQHRRSLDDQSDSFRPPYNKRG--LT 246

Query: 249 RRS-------QDEADFMSSGRPSIDRIFPSVYDSLDMGGRATPPRMSSSTDLDLTQSFES 308
            RS         E  F SSGRPSI+R  PS+YD+ D     TPPR+S+ +D+D   SFES
Sbjct: 247 GRSYGELSIPDSEISFNSSGRPSIERNSPSLYDNSD--PNRTPPRLSNFSDVDYC-SFES 306

Query: 309 LPMGRLSMDMNFPSEFSSISQDSDRLSISSQSLEDVEAEMRRLKLELKQTMEMYSTACKE 368
           +  GR SMD++ P+ FS+ S +++R S +SQ  +DVEAEMRRLKLELKQTMEMYSTACKE
Sbjct: 307 MTFGRRSMDLSSPTAFSTGSFENERFSSASQGGDDVEAEMRRLKLELKQTMEMYSTACKE 366

Query: 369 ALTAKQKAVELQRWKLEEEQRIEEARLAGEAALALAEQEKAKXXXXXXXXXXXXXXXXXX 428
           ALTAK KA ELQRWKL EE++ EEA+LA EAALA+AE+E   XXXXXXXXXX        
Sbjct: 367 ALTAKHKATELQRWKLAEERKFEEAKLAEEAALAIAEKEXXXXXXXXXXXXXAQRIADIE 426

Query: 429 XXXXXXXXXXXXXXXXXKKRAIDALSQSDIRYRKYTIEEIEEATEFFAESRKIGEGGYGP 488
                            + +A++AL++ D+RYRKY+IEEIE+ATEFF +  KIGEG YGP
Sbjct: 427 SRKRVDAETKALKESEARTKAVNALAK-DVRYRKYSIEEIEDATEFFDDKYKIGEGSYGP 486

Query: 489 VYKCDLDHTPVAIKVLRPDATQGRSQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFM 548
           VYKC LDHTPVA+K LRPDA QGRSQF++EVEVL  IRHPNMVLLLGACPE GCLVYEFM
Sbjct: 487 VYKCYLDHTPVAVKALRPDAAQGRSQFQKEVEVLCSIRHPNMVLLLGACPECGCLVYEFM 546

Query: 549 ANGSLEDRLLQRGNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILLDRN 608
           ANGSLEDRL ++G++P LSWQ RF+IA EIGT LLFLHQTKPEPLVHRDLKPANILLDRN
Sbjct: 547 ANGSLEDRLFRQGDSPALSWQTRFRIAAEIGTVLLFLHQTKPEPLVHRDLKPANILLDRN 606

Query: 609 FVSKISDVGLARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMF 668
           FVSK++DVGLARLVPPSVA+ VTQY MTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMF
Sbjct: 607 FVSKLADVGLARLVPPSVANTVTQYHMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMF 666

Query: 669 LQLITSRPPMGLTHHVERAIENDMFANILDPAVTDWPVEEALSFAKMSLKCAELRRKDRP 726
           LQLIT +PPMGLTH+VERA+E     ++LDPAV+DWPVE+   FAK++LKCAE+RRKDRP
Sbjct: 667 LQLITGKPPMGLTHYVERALEKGNLKDLLDPAVSDWPVEDTTEFAKLALKCAEIRRKDRP 726

BLAST of Carg21184-RA vs. TAIR10
Match: AT2G24370.1 (Protein kinase protein with adenine nucleotide alpha hydrolases-like domain)

HSP 1 Score: 821.6 bits (2121), Expect = 3.8e-238
Identity = 470/787 (59.72%), Postives = 557/787 (70.78%), Query Frame = 0

Query: 1   MWLPRYSLDRKERLNVNGFVAVAIDKDKGSQGALKWAIDHLLRRGQTVFLVHVKLR---- 60
           MW+P+ +    +    +G VAVAIDKDK SQ ALKWA+D+LL+RGQ+V LVHVKLR    
Sbjct: 1   MWMPKNNNVGVKEGGSSGVVAVAIDKDKSSQHALKWAVDNLLQRGQSVILVHVKLRPSPL 60

Query: 61  ----SLSNSSLKMKS-------DPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKAL 120
               SL  SS K+         DPE   +E+FLPFRCFCTRKDIQC+DV+LE++D+AKAL
Sbjct: 61  NNSASLHASSAKLSQDSSLVCRDPEGISKEIFLPFRCFCTRKDIQCQDVLLEESDVAKAL 120

Query: 121 TEYAANTAIENIVVGASSKSGFLRF-KATDVPGNVSKGAPDFCNVFVISKGKIQSMRSAS 180
            EY    AIE +VVG+SSK GFLRF K TD+PG+++K APDFC V++ISKGKIQ+MRSAS
Sbjct: 121 VEYVNQAAIEVLVVGSSSKGGFLRFNKPTDIPGSITKNAPDFCTVYIISKGKIQTMRSAS 180

Query: 181 RPAPVVNPLQSQLVQQSSTKSIRSDYQFPQTTSARASLDKRPF----------------- 240
           R AP+  PL+S +       S++     P T++     D+R F                 
Sbjct: 181 RSAPMTAPLRSSV----QPPSLKPPQPMPSTSANSLRADRRSFESNXXXXXXXXXXXXXX 240

Query: 241 ----------------VLPRRS-----------QDEADFMSSGRPSIDRIFPSVYDSLDM 300
                              RR            + +  F+SSGRPSIDRIFPS+YD+ D 
Sbjct: 241 XXXXXXXXXXXXSFRSPFTRRGNGRSYGDLTVPESDISFISSGRPSIDRIFPSLYDNNDP 300

Query: 301 GGRATPPRMSSSTDLDLTQSF-ESLPMGRLSMDMNFPSEFSSISQDSDRLSISSQSLEDV 360
               TPPR+S+ +D+D + S  +S   GR S+DMN P++F     +S+R S +SQS++DV
Sbjct: 301 S--RTPPRLSNFSDMDYSSSLDQSSNYGRRSVDMNSPTDF-----ESERFSSASQSIDDV 360

Query: 361 EAEMRRLKLELKQTMEMYSTACKEALTAKQKAVELQRWKLEEEQRIEEARLAGEAALALA 420
           EAEMRRLKLELKQTMEMYSTACKEAL                              LA+A
Sbjct: 361 EAEMRRLKLELKQTMEMYSTACKEALXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLAIA 420

Query: 421 EQEKAKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKKRAIDALSQSDIRYRKYT 480
           E+    XXXXXXXXXXXXXXXXXXXXX              K +A+ AL+ SD+RYRKY+
Sbjct: 421 EKXXXXXXXXXXXXXXXXXXXXXXXXXRVNAEMKALKESEEKTKALTALANSDVRYRKYS 480

Query: 481 IEEIEEATEFFAESRKIGEGGYGPVYKCDLDHTPVAIKVLRPDATQGRSQFKQEVEVLSC 540
           IE+IE ATEFFAE  KIGEGGYGPVYKC LDHTPVA+KVLRPDA QGRSQF+QEVEVLSC
Sbjct: 481 IEDIELATEFFAEKYKIGEGGYGPVYKCYLDHTPVAVKVLRPDAAQGRSQFQQEVEVLSC 540

Query: 541 IRHPNMVLLLGACPEYGCLVYEFMANGSLEDRLLQRGNTPPLSWQLRFQIAGEIGTALLF 600
           IRHPNMVLLLGACPE GCLVYEFMANGSLEDRL + GN+PPLSWQ+RF+IA EIGT LLF
Sbjct: 541 IRHPNMVLLLGACPECGCLVYEFMANGSLEDRLFRLGNSPPLSWQMRFRIAAEIGTGLLF 600

Query: 601 LHQTKPEPLVHRDLKPANILLDRNFVSKISDVGLARLVPPSVADNVTQYRMTSTAGTFCY 660
           LHQ KPEPLVHRDLKP NILLDRNFVSKISDVGLARLVPP+VAD VTQYRMTSTAGTFCY
Sbjct: 601 LHQAKPEPLVHRDLKPGNILLDRNFVSKISDVGLARLVPPTVADTVTQYRMTSTAGTFCY 660

Query: 661 IDPEYQQTGMLGVKSDIYSLGIMFLQLITSRPPMGLTHHVERAIENDMFANILDPAVTDW 720
           IDPEYQQTGMLGVKSDIYSLGIMFLQLIT++PPMGLTH+VERA+E     ++LDP V+DW
Sbjct: 661 IDPEYQQTGMLGVKSDIYSLGIMFLQLITAKPPMGLTHYVERALEKGTLVDLLDPVVSDW 720

Query: 721 PVEEALSFAKMSLKCAELRRKDRPDLGKEILPELNRLRTIAEDYIHPTLMGDISSPTRSS 727
           P+E+   FAK++LKCAELRRKDRPDL K ILPELNRLRT+A++  H  ++G+   P+ + 
Sbjct: 721 PMEDTEEFAKLALKCAELRRKDRPDLAKVILPELNRLRTLADESSHSVVVGNSPLPSPTG 776

BLAST of Carg21184-RA vs. TAIR10
Match: AT1G16760.1 (Protein kinase protein with adenine nucleotide alpha hydrolases-like domain)

HSP 1 Score: 724.2 bits (1868), Expect = 8.2e-209
Identity = 405/759 (53.36%), Postives = 515/759 (67.85%), Query Frame = 0

Query: 1   MWLPRYSLDRKERLNVNGFVAVAIDKDKGSQGALKWAIDHLLRRGQTVFLVHVKLRSLSN 60
           MWLP+   D  ++   NG VA+AIDKDK SQ A+KW +++L  RGQT+ L+HV  +S S+
Sbjct: 1   MWLPK--ADATKKGTRNGSVAIAIDKDKSSQNAIKWTLENLATRGQTLALIHVLPKSQSS 60

Query: 61  ----SSLKMKSDPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIEN 120
                 +  K   E   ++LF+ F C+C+RK+I C DVVLED D  KA+ EY   +AIEN
Sbjct: 61  LDIEEGITHKQQMEKQTKDLFVSFHCYCSRKEIHCLDVVLEDVDKVKAIVEYVTVSAIEN 120

Query: 121 IVVGASSKSGFLRFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVNPLQSQ 180
           +V+GA S++ F+R   TD+P +VSK APDFCNV+VISKGKI S+R+ASR AP  +P    
Sbjct: 121 LVLGAPSRNSFMRRFKTDLPTSVSKAAPDFCNVYVISKGKISSLRNASRLAP-YHPSVLS 180

Query: 181 LVQQSSTKSIRSDYQFPQTTS------------ARASLDKRP----------------FV 240
            V    T +I   ++   T +             R  L K P                F 
Sbjct: 181 EVDDHETIAIERKHKTANTPALPKGRRSIDSDVTRLGLPKPPHGHMKLMGDFSDSESEFS 240

Query: 241 LPRRSQDEA---DFMSSGRPSIDRIFPSVYDSLDMGGRATPPRMSSSTDLDLTQSFESLP 300
               SQ E+    F+SSGRPS+DR   S + + D+   A   RMS+S++    QS  S  
Sbjct: 241 FISASQQESSDLSFISSGRPSVDR---SSF-TYDLPESARTSRMSTSSE----QSIGSHR 300

Query: 301 MGRLSMDMNFPSEFSSISQDSDR--LSISSQSLEDVEAEMRRLKLELKQTMEMYSTACKE 360
           +G    D+++ +  SS+S +S R   S SSQSL DVEA+M+RL+LELKQTM+MYS+AC+E
Sbjct: 301 LGIKFTDLSYLNGSSSVSDESGRTSCSFSSQSLNDVEAQMKRLRLELKQTMDMYSSACRE 360

Query: 361 ALTAKQKAVELQRWKLEEEQRIEEARLAGEAALALAEQEKAKXXXXXXXXXXXXXXXXXX 420
           ALTA+ +A ELQ+ + EEE+R+EE ++  E A+++ E E+AK                  
Sbjct: 361 ALTARNEATELQKLRTEEERRLEELKMTEETAMSIVENERAKAKTALEAAEAANRLAEVE 420

Query: 421 XXXXXXXXXXXXXXXXXKKRAIDALSQSDIRYRKYTIEEIEEATEFFAESRKIGEGGYGP 480
                             K +      S +RYRKY+++EIEE T  FAESRK+GEGGYGP
Sbjct: 421 AKRRVHAEMKVL------KESDSFSRHSIVRYRKYSVQEIEEGTANFAESRKVGEGGYGP 480

Query: 481 VYKCDLDHTPVAIKVLRPDATQGRSQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFM 540
           V++  LDHT VA+KVLRPDA QGRSQF +EVEVLSCIRHPNMVLLLGACPEYG LVYE+M
Sbjct: 481 VFRGHLDHTSVAVKVLRPDAAQGRSQFHKEVEVLSCIRHPNMVLLLGACPEYGILVYEYM 540

Query: 541 ANGSLEDRLLQRGNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILLDRN 600
           A GSL+DRL +RGNTPP+SWQLRF+IA EI T LLFLHQTKPEP+VHRDLKP N+LLD N
Sbjct: 541 ARGSLDDRLFRRGNTPPISWQLRFRIAAEIATGLLFLHQTKPEPIVHRDLKPGNVLLDHN 600

Query: 601 FVSKISDVGLARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMF 660
           +VSKISDVGLARLV P+VA+NVTQYR+TS AGTFCYIDPEYQQTGMLGVKSD+YSLGIM 
Sbjct: 601 YVSKISDVGLARLV-PAVAENVTQYRVTSAAGTFCYIDPEYQQTGMLGVKSDVYSLGIML 660

Query: 661 LQLITSRPPMGLTHHVERAIENDMFANILDPAVTDWPVEEALSFAKMSLKCAELRRKDRP 720
           LQL+T++ PMGL ++VE+AIE     ++LDPAV DWP+EEALS AK+SL+CAELRRKDRP
Sbjct: 661 LQLLTAKQPMGLAYYVEQAIEEGTLKDMLDPAVPDWPLEEALSLAKLSLQCAELRRKDRP 720

BLAST of Carg21184-RA vs. TAIR10
Match: AT1G78940.2 (Protein kinase protein with adenine nucleotide alpha hydrolases-like domain)

HSP 1 Score: 719.2 bits (1855), Expect = 2.6e-207
Identity = 417/753 (55.38%), Postives = 524/753 (69.59%), Query Frame = 0

Query: 1   MWLPRYSLDRKERLNVNGFVAVAIDKDKGSQGALKWAIDHLLRRGQTVFLVHVKLRSLSN 60
           MWLP+ +  +KE    +G VAVAIDKDKGSQ ALKW ID+L  RGQT+ L+HV  RS S+
Sbjct: 1   MWLPKANGAKKE--TGSGSVAVAIDKDKGSQHALKWTIDNLASRGQTISLIHVLCRSHSS 60

Query: 61  SSL-----KMKSDPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIE 120
           S L     + K   E   ++LF+ F C+C+RK+I C+D++LED D  +A+TEY +++AIE
Sbjct: 61  SDLEEGTPQQKQQMEKIAKDLFVSFHCYCSRKEINCRDILLEDADKVRAITEYVSSSAIE 120

Query: 121 NIVVGASSKSGFLRFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVNPLQS 180
           N+VVG++S++GF+R   TD+P  VSK APDFCNV+VISKGKI S+R+ASRPAP  N +Q 
Sbjct: 121 NLVVGSASRNGFMRRFKTDLPTTVSKSAPDFCNVYVISKGKIASVRNASRPAPYQNSMQQ 180

Query: 181 QLVQQSSTKSIRSDYQFPQTTSARASLDKRP--FVLPRRS-----QDEADFMSSGRPSID 240
             +      +     ++    ++  S   RP   V   RS     +   DF  S    + 
Sbjct: 181 CEIDNHHPHTPDKAPKYHDHPNSAGSTPSRPRKSVEADRSPLVKRKPYGDFYDSD-SDLS 240

Query: 241 RIFPSVYD-------------------SLDMGGRATPPRMSSSTDLDLTQSFESLPMGRL 300
            I PS +                    SLD    A   RMS+S++    QS  S  +G  
Sbjct: 241 FISPSSHRXXXXXXXXXXXXSVERSSFSLDFPESARTSRMSTSSE----QSIGSNRLGIK 300

Query: 301 SMDMNFPSEFSSISQDSDRL-SISSQSLEDVEAEMRRLKLELKQTMEMYSTACKEALTAK 360
             D +F +E S+ S++S R  S SSQSL+DVEAEM+RL+LELKQTM+MYSTACKEAL+A+
Sbjct: 301 FSDPDFLNESSTFSEESGRTSSYSSQSLDDVEAEMKRLRLELKQTMDMYSTACKEALSAR 360

Query: 361 QKAVELQRWKLEEEQRIEEARLAGEAALALAEQEKAKXXXXXXXXXXXXXXXXXXXXXXX 420
           Q+A ELQ+ + E                ++ E+E+A       XXXXXXXXXXXXXXXX 
Sbjct: 361 QQATELQKLRTEXXXXXXXXXXXXXXXXSIVEKERA-------XXXXXXXXXXXXXXXXE 420

Query: 421 XXXXXXXXXXXXKKRAIDALSQSDIRYRKYTIEEIEEATEFFAESRKIGEGGYGPVYKCD 480
                         +  D+ S+  +RYRKYT++EIEEAT  FAES+K+GEGGYGPV++  
Sbjct: 421 VESKRRLTAEMKTMKESDSFSRGFVRYRKYTVDEIEEATSNFAESQKVGEGGYGPVFRGF 480

Query: 481 LDHTPVAIKVLRPDATQGRSQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSL 540
           LDHT VA+KVLRPDA QGRSQF++EVEVLSCIRHPNMVLLLGACPE+G LVYE+MA GSL
Sbjct: 481 LDHTSVAVKVLRPDAAQGRSQFQKEVEVLSCIRHPNMVLLLGACPEFGILVYEYMAKGSL 540

Query: 541 EDRLLQRGNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKI 600
           EDRL  RGNTPP++WQLRF+IA EI T LLFLHQTKPEP+VHRDLKP N+LLD N+VSKI
Sbjct: 541 EDRLFMRGNTPPITWQLRFRIAAEIATGLLFLHQTKPEPIVHRDLKPGNVLLDYNYVSKI 600

Query: 601 SDVGLARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLIT 660
           SDVGLARLV P+VA+NVTQYR+TS AGTFCYIDPEYQQTGMLGVKSD+YSLGIM LQ++T
Sbjct: 601 SDVGLARLV-PAVAENVTQYRVTSAAGTFCYIDPEYQQTGMLGVKSDVYSLGIMLLQILT 660

Query: 661 SRPPMGLTHHVERAIENDMFANILDPAVTDWPVEEALSFAKMSLKCAELRRKDRPDLGKE 720
           ++ PMGL ++VE+AIE     ++LDPAV DWP+EEALS AK+SL+CAELRRKDRPDLGKE
Sbjct: 661 AKQPMGLAYYVEQAIEEGTLKDMLDPAVPDWPIEEALSLAKLSLQCAELRRKDRPDLGKE 720

Query: 721 ILPELNRLRTIAEDYIHPTLMGDISSPTRSSSQ 722
           ILPELNRLR I E+ +           + ++SQ
Sbjct: 721 ILPELNRLREIGEESLESVFYAGNQGKSPNTSQ 738

BLAST of Carg21184-RA vs. TAIR10
Match: AT5G35380.1 (Protein kinase protein with adenine nucleotide alpha hydrolases-like domain)

HSP 1 Score: 711.1 bits (1834), Expect = 7.2e-205
Identity = 405/713 (56.80%), Postives = 501/713 (70.27%), Query Frame = 0

Query: 20  VAVAIDKDKGSQGALKWAIDHLLRRGQTVFLVHVKLRSLSN--SSLKMKSDPEAGVRELF 79
           + +AID+DK SQ ALKWA+ +LL RGQT+ L+HVKL+  S+   S    S P     ELF
Sbjct: 12  ITLAIDRDKESQNALKWAVSNLLSRGQTLTLLHVKLKQPSSLPYSGSNFSKPGDDPSELF 71

Query: 80  LPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIVVGASSKSGFLRFKATDVPG 139
           LPFRC+C RKDI C+DVV+ED   AK + +Y    AIE +++G SSK   LRFKA DV  
Sbjct: 72  LPFRCYCARKDINCQDVVVEDVSAAKGIVDYVQQNAIETLILG-SSKMTLLRFKAADVSS 131

Query: 140 NVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVN--PLQSQLVQQSSTKSIRSDYQFPQT 199
            V K AP FC V+VISKGKI  +RSA+   P  N   ++     Q+S  ++    Q  Q 
Sbjct: 132 TVMKKAPSFCTVYVISKGKISFLRSATSSPPHSNMPSMRHHSHAQTSNMNVERRQQTMQR 191

Query: 200 TSARASLD-KRPF---VLPRRSQDEADFMSSGRPSIDRIFPSVYDSLDMGGRATPPRMSS 259
           T     ++ KR +     P  +  +  F+SSGRPS+D++FPS+YD +D+      PR+S 
Sbjct: 192 THDEIKIEIKRGYEGVYQPSITDSDISFVSSGRPSVDQMFPSLYDDVDV------PRLSV 251

Query: 260 STDLDLTQSFESLPMGRLSMDMNFP-SEFSSISQDSDRLSISSQSLEDVEAEMRRLKLEL 319
           +++    +   +    + S+D+  P +  SS S +S R S S Q  +++E EMRRLK+EL
Sbjct: 252 TSEYGENRLSFATTYSKQSIDLGSPYAPNSSTSFESGRQSFSLQGQDELETEMRRLKMEL 311

Query: 320 KQTMEMYSTACKEALTAKQKAVELQRWKLEEEQRIEEARLAGEAALALAEQEKAKXXXXX 379
           K TMEMY++ACKEA++AK+ A EL +WK E+E ++EE RL+ EAA+         XXXXX
Sbjct: 312 KHTMEMYNSACKEAISAKKAANELLKWKAEKEHKLEEVRLSKEAAM-----XXXXXXXXX 371

Query: 380 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKKRAIDALSQSDIRYRKYTIEEIEEATEFF 439
           XXXXXXX                       KKRA+     S +RYRKYTIEEIEEATE F
Sbjct: 372 XXXXXXXAVAAQKLSDLEAEKRKHIETVDEKKRAV-----SSLRYRKYTIEEIEEATEDF 431

Query: 440 AESRKIGEGGYGPVYKCDLDHTPVAIKVLRPDATQGRSQFKQEVEVLSCIRHPNMVLLLG 499
           + SRK+GEGGYGPVYK  LD+T VAIKVLRPDA QGRSQF++EVEVL+C+RHPNMVLLLG
Sbjct: 432 SPSRKVGEGGYGPVYKGTLDYTKVAIKVLRPDAAQGRSQFQREVEVLTCMRHPNMVLLLG 491

Query: 500 ACPEYGCLVYEFMANGSLEDRLLQRGNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVH 559
           ACPEYGCLVYE+MANGSL+D L +RGN+P LSWQLRF+IA EI T L FLHQ KPEPLVH
Sbjct: 492 ACPEYGCLVYEYMANGSLDDCLFRRGNSPILSWQLRFRIASEIATGLHFLHQMKPEPLVH 551

Query: 560 RDLKPANILLDRNFVSKISDVGLARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGML 619
           RDLKP NILLD++FVSKISDVGLARLVPPSVAD  TQYRMTSTAGTF YIDPEYQQTGML
Sbjct: 552 RDLKPGNILLDQHFVSKISDVGLARLVPPSVADTATQYRMTSTAGTFFYIDPEYQQTGML 611

Query: 620 GVKSDIYSLGIMFLQLITSRPPMGLTHHVERAIENDMFANILDPAVTDWPVEEALSFAKM 679
           G KSDIYS GIM LQ++T++PPMGLTHHVE+AIE   FA +LDPAV DWP EEAL+ AK+
Sbjct: 612 GTKSDIYSFGIMLLQILTAKPPMGLTHHVEKAIEKGTFAEMLDPAVPDWPFEEALAAAKL 671

Query: 680 SLKCAELRRKDRPDLGKEILPELNRLRTIAEDYIHPTLMGDISSPTRSSSQVS 724
           +L+CA+LRRKDRPDLG  +LPEL +LR +AE+ I   +     SP RSS   +
Sbjct: 672 ALQCAKLRRKDRPDLGNIVLPELKKLRDLAEESIKFGVRQ--PSPIRSSGSAT 705

BLAST of Carg21184-RA vs. Swiss-Prot
Match: sp|Q9FKG5|PUB51_ARATH (U-box domain-containing protein 51 OS=Arabidopsis thaliana OX=3702 GN=PUB51 PE=2 SV=2)

HSP 1 Score: 390.2 bits (1001), Expect = 5.0e-107
Identity = 301/712 (42.28%), Postives = 410/712 (57.58%), Query Frame = 0

Query: 20  VAVAI-DKDKGSQGALKWAIDHLLRRGQTVF-LVHVKLRSLSNSSLK--------MKSDP 79
           VAVAI   +  ++G ++WA+     +   VF L+HV+ R  ++ S           K D 
Sbjct: 8   VAVAIKGNNSKTKGVVRWALQEFASQEHVVFKLLHVQPRDSNSVSTTRKDLTTSVYKKDV 67

Query: 80  EAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIVVGASSKSGF-L 139
           +   RE+ LP R     +++Q   +VLE  DIA A+++   +  I  +V+GASS   F  
Sbjct: 68  DRKTREMLLPSRDMFVHREVQLDIMVLESDDIADAISKAVQDHGISELVIGASSSIIFSW 127

Query: 140 RFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRP-----APVVNPLQSQLVQQSST 199
           + K +++   ++   P FC+V VISKGK+ ++R +        A   +  +      S T
Sbjct: 128 KLKRSNLSSRIADATPRFCSVHVISKGKLLNVRKSDMDTETSIADDRSESRFSSDSHSGT 187

Query: 200 KSIRSDYQFPQTTSARASLDKRPFVLPRRSQDEADFMSSGRPSIDRIFPSVYDSLDMGGR 259
            S  S +QF    S+   L +R   L   +Q     +                SLD+   
Sbjct: 188 VSSTSSHQF----SSTPLLFQRIQALTTVNQKVGTNIGKQXXXXXXXXXXXAGSLDVDES 247

Query: 260 ATPPR----MSSSTDLDLTQSFESLPMGRLSMDMNFPSEFSSISQDSDRLSISSQSLEDV 319
               +     +SS+ +    S  S      S   +   E SS S  SD  S SSQ  +D 
Sbjct: 248 KLLNQKGFYRTSSSGIGYGGSDIS------SWRSSQMEEASSSSTYSDPTSSSSQIHKDF 307

Query: 320 EAEMRRLKLELKQTMEMYSTACKEALTAKQKAVELQRWKLEEEQRIEEARLAGEAALALA 379
           E E  +LK+EL+    MY+ A  E + A +K  +L + + EE  R++   +  E A  + 
Sbjct: 308 ELE--KLKIELRHIKGMYAVAQSEVIDASKKMQDLNQRRSEEATRLKNLTIREEEADEVV 367

Query: 380 EQEKAKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKKRAIDALSQSDI---RYR 439
           E E+  XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX     DAL    +   +Y 
Sbjct: 368 EMERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLEDALEGGPLQRQQYM 427

Query: 440 KYTIEEIEEATEFFAESRKIGEGGYGPVYKCDLDHTPVAIKVLRPDATQGRSQFKQEVEV 499
           K+  EEI EAT  F++  KIG GGYG VY+C+L HT VA+KVL  D +    QF QE+E+
Sbjct: 428 KFEWEEIVEATSSFSDELKIGVGGYGSVYRCNLHHTTVAVKVLHSDKSSLTKQFHQELEI 487

Query: 500 LSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDRLLQR------GNTPPLSWQLRFQIA 559
           LS IRHP+++LLLGACPE G LVYE+M NGSLE+RL++R         PPL W  RF+IA
Sbjct: 488 LSKIRHPHLLLLLGACPERGSLVYEYMHNGSLEERLMKRRPNVDTPQPPPLRWFERFRIA 547

Query: 560 GEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDVGLARLVPPSVADNVTQYRM 619
            EI +AL FLH  +P P+VHRDLKPANILLDRN VSKI DVGL+++V    +   T +  
Sbjct: 548 WEIASALYFLHTNEPRPIVHRDLKPANILLDRNNVSKIGDVGLSKMVNLDPSHASTVFNE 607

Query: 620 TSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRPPMGLTHHVERAI--ENDMF 679
           T   GTF YIDPEYQ+TG++  +SDIY+ GI+ LQL+T+R  MGL H +E+A+  +   F
Sbjct: 608 TGPVGTFFYIDPEYQRTGVVTPESDIYAFGIILLQLVTARSAMGLAHSIEKALRDQTGKF 667

Query: 680 ANILDPAVTDWPVEEALSFAKMSLKCAELRRKDRPDLGKEILPELNRLRTIA 701
             ILD    DWPV+EA     + L+CAE+R++DRPDLGKEILP L RL+ +A
Sbjct: 668 TEILDKTAGDWPVKEAKEMVMIGLRCAEMRKRDRPDLGKEILPVLERLKEVA 707

BLAST of Carg21184-RA vs. Swiss-Prot
Match: sp|Q9FKG6|PUB52_ARATH (U-box domain-containing protein 52 OS=Arabidopsis thaliana OX=3702 GN=PUB52 PE=2 SV=1)

HSP 1 Score: 379.8 bits (974), Expect = 6.8e-104
Identity = 274/756 (36.24%), Postives = 392/756 (51.85%), Query Frame = 0

Query: 28  KGSQGALKWAIDHLLRRGQTVF-LVHVK------------------LRSLSNSSLKMKSD 87
           K S+  + WA++  +  G T F L++V+                  LR    S+ K + D
Sbjct: 32  KKSKYVVFWALEKFIPEGFTDFKLLYVRPPVSYIPTPMGIAVAVSELREDVVSAYKQELD 91

Query: 88  PEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIVVGASSKSGFL 147
             A   E+  P++    R+ +Q + ++L+  + A A+ E  A T +  +V+G S +  F 
Sbjct: 92  WSA--NEMLRPYKKMFERRKVQVEVLLLDSLEPAAAIAEEIAGTGVTKLVIGMSLRGFFS 151

Query: 148 RFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVNPLQSQLVQQSSTKSIRS 207
           R    D+   ++   P FC V+VISKGK+ S+R +               +  ++ SIR 
Sbjct: 152 R--KIDMSSLIATAVPRFCTVYVISKGKLASVRPS---------------ESDASGSIR- 211

Query: 208 DYQFPQTTSARASLDKRPFVLPRRSQDEADFMSSGRPSIDRIFPSVYDSLDMGGRATPPR 267
              F +++S   S D     LP   QD    +S  +  +    P++  S+     A    
Sbjct: 212 ---FERSSSTSGSTDSPR--LPPEYQDFLSAVSEAQSRVSPFSPALKHSMGSNAVAQMDT 271

Query: 268 MSSSTDLD--------------------LTQSFE-SLPMGR------------------- 327
            SS TD +                      +SF  S PMG                    
Sbjct: 272 SSSGTDQEEVSTGRGMEIVHSGIEGKKNKDESFSASFPMGTEAYNSMSWTSKWRDHEDRR 331

Query: 328 -------------LSMDMN--FPSEFSSISQDSDRLS---ISSQSLED----VEAEMRRL 387
                        ++MD     P  +S +S  +  +S   +S  S+ D    +  E+ +L
Sbjct: 332 EMRSSSSSNNHDLVNMDWGAVVPENYSWVSHTASHMSDGLLSVHSITDNQVNLNFEIEKL 391

Query: 388 KLELKQTMEMYSTACKEALTAKQKAVELQRWKLEEEQRIEEARLAGEAALALAEQEKAKX 447
           + ELK   EMY+ A  E + A +K  EL + + EE +++ E +                X
Sbjct: 392 RAELKHVQEMYAMAQTETVGASKKLTELNQRRFEESEKLVELKEXXXXXXXXXXXXXXXX 451

Query: 448 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKKRAIDALSQSDIRYRKYTIEEIEEA 507
           XXXXXXXXXXXXXXXX                  K +   +L    ++Y+ YT EEI  A
Sbjct: 452 XXXXXXXXXXXXXXXXEALHRREAEFKAERDAREKDKLQASLVSPGVQYQHYTWEEIAAA 511

Query: 508 TEFFAESRKIGEGGYGPVYKCDLDHTPVAIKVLRPDATQGRSQFKQEVEVLSCIRHPNMV 567
           T  FAE+ KIG G YG VYKC+L HT  A+KVL    TQ   QF QE+E+LS IRHP++V
Sbjct: 512 TSDFAENLKIGIGAYGSVYKCNLHHTTGAVKVLHAGETQLSKQFDQELEILSKIRHPHLV 571

Query: 568 LLLGACPEYGCLVYEFMANGSLEDRLLQRGNTPPLSWQLRFQIAGEIGTALLFLHQTKPE 627
           LLLGACPE GCLVYE+M NGSL+DRL+   +TPP+ W  RF+IA E+ +AL+FLH++KP 
Sbjct: 572 LLLGACPERGCLVYEYMDNGSLDDRLMLVNDTPPIPWFERFRIALEVASALVFLHKSKPR 631

Query: 628 PLVHRDLKPANILLDRNFVSKISDVGLARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQ 687
           P++HRDLKP NILLD NFVSK+ DVGL+ +V      + T ++ TS  GT CYIDPEYQ+
Sbjct: 632 PIIHRDLKPGNILLDHNFVSKLGDVGLSTMVNQDDVSSRTIFKQTSPVGTLCYIDPEYQR 691

Query: 688 TGMLGVKSDIYSLGIMFLQLITSRPPMGLTHHVERAIENDM-FANILDPAVTDWPVEEAL 702
           TG++  KSD+YSLG++ LQLIT++P + +TH VE AI +D  F  ILD     WP+ +  
Sbjct: 692 TGIISPKSDVYSLGVVILQLITAKPAIAITHMVEEAIGDDAEFMAILDKKAGSWPISDTR 751

BLAST of Carg21184-RA vs. Swiss-Prot
Match: sp|Q8S8S7|PUB34_ARATH (U-box domain-containing protein 34 OS=Arabidopsis thaliana OX=3702 GN=PUB34 PE=3 SV=1)

HSP 1 Score: 379.4 bits (973), Expect = 8.9e-104
Identity = 259/707 (36.63%), Postives = 357/707 (50.50%), Query Frame = 0

Query: 19  FVAVAI-----DK--DKGSQGALKWAIDHLLRRGQTVFLVHVKLRSLSNSSLKMKSDPEA 78
           FVAVA+     DK    GS+ A++WA+D+LL +     ++HV           + S P  
Sbjct: 23  FVAVAVKGLIGDKLGGAGSRRAVRWAVDNLLPKADKFVMIHV--------IPTITSIPTP 82

Query: 79  GVRELFLPFRCFCTRKD------IQCKDVVLEDTDIAKALTEYAANTAIENIVVGASSKS 138
            +  L        T  D      ++   V +   D+ K   E      ++      S+K 
Sbjct: 83  NILILMFTRMWVVTAGDRLPVEEVEESVVEMYVRDVKKEY-ETVFVPFLKMCKSTRSTKR 142

Query: 139 GF--LRFKATDVPGNVSKGAPDFCNVFVISKGKI--QSMRSASRPAPVVNPLQSQLVQQ- 198
            F   R K T VP  V + AP+ C V+++ K +I  +SM       P  +P  +      
Sbjct: 143 YFRSRRTKGTGVPLTVLRYAPETCEVYIVCKDRITTKSMDPLINREPCTSPHAAATAHDF 202

Query: 199 -----SSTKSIRSDYQFPQTTSARASLD--KRPFVLPRRSQDEADFMSSGRPSIDRIFPS 258
                +S  ++RS    P     R S +   R     R  + EA  ++  +P   +    
Sbjct: 203 LRDWAASFHTLRS----PTLPDPRQSTEAGTRRSASARELRFEALSLTCNKPKTPQ---- 262

Query: 259 VYDSLDMGGRATPPRMSSSTDLDLTQ----SFESLPMGRLSMDMNFPSEFSSISQDSDRL 318
              S       TP         D+ Q     F+       S   N  SE     +     
Sbjct: 263 ---SSKASSATTPEIFRRRRGSDIPQLNYSDFDKTCTKPQSNVENIVSEHRDSDRSPPET 322

Query: 319 SISSQSLEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKAVELQRWKLEEEQRIEEAR 378
           S  S+ +E +E E+ RLK EL+ T+  Y  AC+E  + + K   L    L E +R+  A 
Sbjct: 323 SRKSKKVE-IEEEVERLKNELQSTVFKYKQACEELFSTQNKVKMLSTEYLNESKRVNNAV 382

Query: 379 LAGEAALALAEQEKAKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKKRAIDALS 438
              E     A  EK +                                   KK+ ID L 
Sbjct: 383 EKEELQRNTAALEKERYMKAVKEVETAKALLAREFCQRQIAEVNALRTYLEKKKVIDQLL 442

Query: 439 QSDIRYRKYTIEEIEEATEFFAESRKIGEGGYGPVYKCDLDHTPVAIKVLRPDATQGRSQ 498
            +D RYRKYTIEEI  ATE F+  + IGEGGYG VY+C LD TP A+KV+R D  + + +
Sbjct: 443 GTDHRYRKYTIEEIVTATEGFSPEKVIGEGGYGKVYQCSLDSTPAAVKVVRLDTPEKKQE 502

Query: 499 FKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDRLLQRGNTPPLSWQLRFQI 558
           F +EVEVLS +RHP++VLLLGACPE GCLVYE++ NGSLE+ +  R N PPL W +RF++
Sbjct: 503 FLKEVEVLSQLRHPHVVLLLGACPENGCLVYEYLENGSLEEYIFHRKNKPPLPWFIRFRV 562

Query: 559 AGEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDVGLARLVPPSVADNVTQYR 618
             E+   L FLH +KPEP+VHRDLKP NILL+RN+VSKI+DVGLA+LV     DNVT YR
Sbjct: 563 IFEVACGLAFLHSSKPEPIVHRDLKPGNILLNRNYVSKIADVGLAKLVTDVAPDNVTMYR 622

Query: 619 MTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRPPMGLTHHVERAIENDMFA 678
            +  AGT  YIDPEY +TG +  KSD+Y+ GI+ LQL+T+R P G+   VE A++     
Sbjct: 623 NSVLAGTLHYIDPEYHRTGTIRPKSDLYAFGIIILQLLTARNPSGIVPAVENAVKKGTLT 682

Query: 679 NILDPAVTDWPVEEALSFAKMSLKCAELRRKDRPDLGKEILPELNRL 697
            +LD +VTDWP+ E    A++ LKCAE R +DRPDL  E++P L RL
Sbjct: 683 EMLDKSVTDWPLAETEELARIGLKCAEFRCRDRPDLKSEVIPVLKRL 708

BLAST of Carg21184-RA vs. Swiss-Prot
Match: sp|Q9SW11|PUB35_ARATH (U-box domain-containing protein 35 OS=Arabidopsis thaliana OX=3702 GN=PUB35 PE=2 SV=2)

HSP 1 Score: 377.9 bits (969), Expect = 2.6e-103
Identity = 293/725 (40.41%), Postives = 390/725 (53.79%), Query Frame = 0

Query: 30  SQGALKWAIDHLLRRGQTVF-LVHVKLRSLSNSSLKMKSDPEAGVR-------------- 89
           S+  + WAI+     G   F L+H+     S  +    + P + VR              
Sbjct: 32  SKYVVTWAIEKFATEGNVGFKLLHIHPMITSVPTPMGNAIPISEVRDDVVTAYRQEILWQ 91

Query: 90  --ELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIVVGASSKSGFLRFKA 149
             E+  P+     R+ +  + +V+E  ++A A+ E     +I+ IV+G SS+S F R   
Sbjct: 92  SEEMLKPYTKLFVRRKVAVEVLVIESDNVAAAIAEEVTRDSIDRIVIGGSSRSFFSR--K 151

Query: 150 TDVPGNVSKGAPDFCNVFVISKGKIQSMR-SASRPAPVVNPLQSQLVQQSSTKSIRSDYQ 209
            D+   +S   P+FC V+V+SKGK+  +R S S     +    S+    SS  S  +   
Sbjct: 152 ADICSVISALMPNFCTVYVVSKGKLSCVRPSDSDGNATIREDGSERTNSSSGSSGPTSDS 211

Query: 210 FPQTTSARASLDKRPFVLP-RRSQ--------------------DEADFMSSGRPSIDRI 269
               +SA  S   RP  LP RR Q                    DE   MS        +
Sbjct: 212 SDVMSSAHDS-QSRPLSLPVRRMQHFPAIAGQASVPMETSSVGSDETRCMSLDAEEARDV 271

Query: 270 FPSVYDSLDMGGRATPPR----------MSSSTDLDLTQSFESLPMGRLSMDMNFPSEFS 329
                 S D   R TP R           SSS++ +            + +D        
Sbjct: 272 SSINRSSTDTTSRWTPRRRDYEERKEAMSSSSSNREYGNFGTRFSWSGMGVDTTHSRASQ 331

Query: 330 SISQDSDRLSISSQSLEDVEA--EMRRLKLELKQTMEMYSTACKEALTAKQKAVELQRWK 389
             S  SD LS  S +   V    E+ +L+ EL+   EMY+ A  E   A +K  EL + +
Sbjct: 332 QASNMSDALSEQSYTDNQVNLNFEVEKLRAELRHVQEMYAVAQTETFDASRKLGELNQRR 391

Query: 390 LEEEQRIEEARLAGEAALALAEQEKAKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 449
           LEE  +                     XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 392 LEEAIKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 451

Query: 450 XXKKRAIDALSQSDIRYRKYTIEEIEEATEFFAESRKIGEGGYGPVYKCDLDHTPVAIKV 509
           XX ++    L    ++Y+ +  EEI  AT  F+E  KIG G YG VYKC+L HT   +KV
Sbjct: 452 XXXEKLEGTLGSPQLQYQHFAWEEIMAATSSFSEELKIGMGAYGAVYKCNLHHTTAVVKV 511

Query: 510 LRPDATQGRSQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDRLLQRGNT 569
           L+    Q   QF+QE+E+LS IRHP++VLLLGACPE G LVYE+M NGSLEDRL Q  N+
Sbjct: 512 LQSAENQLSKQFQQELEILSKIRHPHLVLLLGACPEQGALVYEYMENGSLEDRLFQVNNS 571

Query: 570 PPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDVGLARLVP 629
           PPL W  RF+IA E+  AL+FLH++KP+P++HRDLKPANILLD NFVSK+ DVGL+ +V 
Sbjct: 572 PPLPWFERFRIAWEVAAALVFLHKSKPKPIIHRDLKPANILLDHNFVSKVGDVGLSTMVQ 631

Query: 630 -PSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRPPMGLTH 689
              ++   T Y+ TS  GT CYIDPEYQ+TG +  KSDIYS G++ LQL+T++P + LTH
Sbjct: 632 VDPLSTKFTIYKQTSPVGTLCYIDPEYQRTGRISSKSDIYSFGMILLQLLTAKPAIALTH 691

Query: 690 HVERAIE-NDMFANILDPAVTDWPVEEALSFAKMSLKCAELRRKDRPDLGKEILPELNRL 702
            VE A++ ND F  ILD    +WP+EE    A ++L C ELR KDRPDL  +ILP L  L
Sbjct: 692 FVESAMDSNDEFLKILDQKAGNWPIEETRELAALALCCTELRGKDRPDLKDQILPALENL 751

BLAST of Carg21184-RA vs. Swiss-Prot
Match: sp|Q9LU47|PUB53_ARATH (Putative U-box domain-containing protein 53 OS=Arabidopsis thaliana OX=3702 GN=PUB53 PE=3 SV=1)

HSP 1 Score: 325.1 bits (832), Expect = 2.0e-87
Identity = 153/294 (52.04%), Postives = 209/294 (71.09%), Query Frame = 0

Query: 413 IRYRKYTIEEIEEATEFFAESRKIGEGGYGPVYKCDLDHTPVAIKVLRPDATQGRSQFKQ 472
           ++Y+++T EEI  AT  F+E  KIG G YG VYKC+L HT  A+KVL    +    QF Q
Sbjct: 443 LQYQEFTWEEIINATSSFSEDLKIGMGAYGDVYKCNLHHTIAAVKVLHSAESSLSKQFDQ 502

Query: 473 EVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDRLLQRGNTPPLSWQLRFQIAGE 532
           E+E+LS IRHP++VLLLGACP++G LVYE+M NGSLEDRL Q  ++ P+ W +R +IA E
Sbjct: 503 ELEILSKIRHPHLVLLLGACPDHGALVYEYMENGSLEDRLFQVNDSQPIPWFVRLRIAWE 562

Query: 533 IGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDVGLARLVPPS--VADNVTQYRM 592
           + +AL+FLH++KP P++HRDLKPANILL+ NFVSK+ DVGL+ ++  +  ++   T Y+ 
Sbjct: 563 VASALVFLHKSKPTPIIHRDLKPANILLNHNFVSKVGDVGLSTMIQAADPLSTKFTMYKQ 622

Query: 593 TSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRPPMGLTHHVERAIEN---DM 652
           TS  GT CYIDPEYQ+TG +  KSD+Y+ G++ LQL+T +  M LT+ VE A+EN   D 
Sbjct: 623 TSPVGTLCYIDPEYQRTGRISPKSDVYAFGMIILQLLTGQQAMALTYTVETAMENNNDDE 682

Query: 653 FANILDPAVTDWPVEEALSFAKMSLKCAELRRKDRPDLGKEILPELNRLRTIAE 702
              ILD    +WP+EE    A ++L+C ELR KDRPDL  +ILP L  L+ +A+
Sbjct: 683 LIQILDEKAGNWPIEETRQLAALALQCTELRSKDRPDLEDQILPVLESLKKVAD 736

BLAST of Carg21184-RA vs. TrEMBL
Match: tr|A0A1S3CQW0|A0A1S3CQW0_CUCME (U-box domain-containing protein 52 OS=Cucumis melo OX=3656 GN=LOC103503739 PE=4 SV=1)

HSP 1 Score: 1171.0 bits (3028), Expect = 0.0e+00
Identity = 637/755 (84.37%), Postives = 682/755 (90.33%), Query Frame = 0

Query: 1   MWLPRYSLDRKERLNVNGFVAVAIDKDKGSQGALKWAIDHLLRRGQTVFLVHVKLRSLSN 60
           MWLPRYSL+RKE+LNVNGFVAVAIDKDKGSQGALKWAIDHLL+RG TVFL+HVKL+S SN
Sbjct: 1   MWLPRYSLERKEKLNVNGFVAVAIDKDKGSQGALKWAIDHLLQRGHTVFLIHVKLKSPSN 60

Query: 61  SSLKMKSDPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIVVG 120
           S+ KMK DPEA V++LFLPFRCFCTRKDIQCKDVVLEDTD+AKA+ EYAANT IENIVVG
Sbjct: 61  STQKMKPDPEASVKDLFLPFRCFCTRKDIQCKDVVLEDTDVAKAIIEYAANTTIENIVVG 120

Query: 121 ASSKSGFLRFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVNPLQSQLVQQ 180
           AS+KSGFLRFKATDVPG++ KGAPDFCNVFVISKGKIQSMRSASRPAP V  L+ QL QQ
Sbjct: 121 ASAKSGFLRFKATDVPGSIVKGAPDFCNVFVISKGKIQSMRSASRPAPTVGALRGQLAQQ 180

Query: 181 SSTKSIRSDYQFPQTTSARASLDKRPFVLPRRSQDEADFM-------------------- 240
            STKS   D+    + SAR SLDK P  LP +SQDEADFM                    
Sbjct: 181 CSTKSDTPDFPVSHSASARVSLDKPPLDLPNKSQDEADFMRSPFTRKGYNYKPSYGDLPM 240

Query: 241 --------SSGRPSIDRIFPSVYDSLDMGGRATPPRMSSSTDLDLTQSFESLPMGRLSMD 300
                   SSGRPSIDRIFP++Y+S DM GRATPP++SSSTDLDLT+SFESL +GR+S+D
Sbjct: 241 QDSDISFVSSGRPSIDRIFPTLYESQDMVGRATPPQISSSTDLDLTRSFESLQLGRMSLD 300

Query: 301 MNFPSEFSSISQDSDRLSISSQSLEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKAV 360
           MNFPSEFSSISQDSDRLSISSQS+EDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKAV
Sbjct: 301 MNFPSEFSSISQDSDRLSISSQSMEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKAV 360

Query: 361 ELQRWKLEEEQRIEEARLAGEAALALAEQEKAKXXXXXXXXXXXXXXXXXXXXXXXXXXX 420
           ELQRWKLEEEQR+EEARLAGEAALALAEQEKAK XXXXXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 361 ELQRWKLEEEQRLEEARLAGEAALALAEQEKAKSXXXXXXXXXXXXXXXXXXXXXXXXXX 420

Query: 421 XXXXXXXXKKRAIDALSQSD-IRYRKYTIEEIEEATEFFAESRKIGEGGYGPVYKCDLDH 480
           XXXXXXX KK+A DAL+Q+D IRYRKYTIEEIE ATEFF+ESR+IGEGGYGPVYKCDLDH
Sbjct: 421 XXXXXXXEKKKAFDALAQTDNIRYRKYTIEEIEAATEFFSESRRIGEGGYGPVYKCDLDH 480

Query: 481 TPVAIKVLRPDATQGRSQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDR 540
           TPVAIKVLRPDATQGRSQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDR
Sbjct: 481 TPVAIKVLRPDATQGRSQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDR 540

Query: 541 LLQRGNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDV 600
           LL+RGNTPPLSWQLRF+IA EIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDV
Sbjct: 541 LLRRGNTPPLSWQLRFKIAAEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDV 600

Query: 601 GLARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRP 660
           GLARLVPPS+AD+VTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRP
Sbjct: 601 GLARLVPPSIADSVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRP 660

Query: 661 PMGLTHHVERAIENDMFANILDPAVTDWPVEEALSFAKMSLKCAELRRKDRPDLGKEILP 720
           PMGLTHHVERAIE  MF  ILDPA+TDWPVEEALSFAK+SLKCAELRRKDRPDLGK ILP
Sbjct: 661 PMGLTHHVERAIEKGMFETILDPAITDWPVEEALSFAKLSLKCAELRRKDRPDLGKAILP 720

Query: 721 ELNRLRTIAEDYIHPTLMGDISSPTRSSSQVSLPL 727
           ELNRLR +AED +HPTLMGD +SP+R++SQVSL L
Sbjct: 721 ELNRLRAMAEDTMHPTLMGD-ASPSRNNSQVSLQL 754

BLAST of Carg21184-RA vs. TrEMBL
Match: tr|A0A0A0LHG8|A0A0A0LHG8_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G882930 PE=4 SV=1)

HSP 1 Score: 1154.0 bits (2984), Expect = 0.0e+00
Identity = 628/756 (83.07%), Postives = 678/756 (89.68%), Query Frame = 0

Query: 1   MWLPRYSLDRKERLNVNGFVAVAIDKDKGSQGALKWAIDHLLRRGQTVFLVHVKLRSLSN 60
           MWLPRYSL+RKERLNVNG VAVAIDKDKGSQGALKWAIDHLL+RG TVFL+HVKL+S SN
Sbjct: 1   MWLPRYSLERKERLNVNGIVAVAIDKDKGSQGALKWAIDHLLQRGHTVFLMHVKLKSPSN 60

Query: 61  SSLKMKSDPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIVVG 120
           S+ KMKSDPEA V++LFLPFRCFCTRKDIQCKD+VLEDTD+AKA+ EYAANT IENIVVG
Sbjct: 61  STQKMKSDPEANVKDLFLPFRCFCTRKDIQCKDIVLEDTDVAKAIIEYAANTTIENIVVG 120

Query: 121 ASSKSGFLRFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVNPLQSQLVQQ 180
           AS++SGFLRFK TD+PG++ KGAPDFCNVFVISKGKIQ+MRSASRPAP V+ L+  L QQ
Sbjct: 121 ASARSGFLRFKVTDIPGSIVKGAPDFCNVFVISKGKIQTMRSASRPAPTVSALRGPLAQQ 180

Query: 181 SSTK-SIRSDYQFPQTTSARASLDKRPFVLPRRSQDEADFM------------------- 240
           SS K S   D+    + +AR SLDK P  LP +SQDEAD+M                   
Sbjct: 181 SSKKLSDTPDFPVSHSATARVSLDKPPLDLPNKSQDEADYMRSPFTRKGYNYKPSYGDLP 240

Query: 241 ---------SSGRPSIDRIFPSVYDSLDMGGRATPPRMSSSTDLDLTQSFESLPMGRLSM 300
                    SSGRPSIDRIFP++Y+S DMGGRATPP++SSSTDLDLT+SFESL +GR+S+
Sbjct: 241 MQDSDISFVSSGRPSIDRIFPTLYESQDMGGRATPPQISSSTDLDLTRSFESLQLGRMSV 300

Query: 301 DMNFPSEFSSISQDSDRLSISSQSLEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKA 360
           DMNFPSEFSSISQDSDRLSISSQS+EDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKA
Sbjct: 301 DMNFPSEFSSISQDSDRLSISSQSMEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKA 360

Query: 361 VELQRWKLEEEQRIEEARLAGEAALALAEQEKAKXXXXXXXXXXXXXXXXXXXXXXXXXX 420
           VELQRWKLEEEQR+EEARLAGEAALALAEQEKAK XXXXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 361 VELQRWKLEEEQRLEEARLAGEAALALAEQEKAKSXXXXXXXXXXXXXXXXXXXXXXXXX 420

Query: 421 XXXXXXXXXKKRAIDALSQSD-IRYRKYTIEEIEEATEFFAESRKIGEGGYGPVYKCDLD 480
           XXXXXXX  KK+A DAL+Q+D IRYRKYTIEEIE ATEFF+ESR+IGEGGYGPVYKCDLD
Sbjct: 421 XXXXXXXEEKKKAYDALAQTDNIRYRKYTIEEIEAATEFFSESRRIGEGGYGPVYKCDLD 480

Query: 481 HTPVAIKVLRPDATQGRSQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLED 540
           HTPVAIKVLRPDATQGRSQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLED
Sbjct: 481 HTPVAIKVLRPDATQGRSQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLED 540

Query: 541 RLLQRGNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISD 600
           RLL+RGNTP LSWQLRF+IA EIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISD
Sbjct: 541 RLLRRGNTPALSWQLRFKIAAEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISD 600

Query: 601 VGLARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSR 660
           VGLARLVPPS+AD+VTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSR
Sbjct: 601 VGLARLVPPSIADSVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSR 660

Query: 661 PPMGLTHHVERAIENDMFANILDPAVTDWPVEEALSFAKMSLKCAELRRKDRPDLGKEIL 720
           PPMGLTHHVER+IE  MF  ILDP +TDWP+EEALSFAK+SLKCAELRRKDRPDLGK IL
Sbjct: 661 PPMGLTHHVERSIEKGMFDTILDPIITDWPIEEALSFAKLSLKCAELRRKDRPDLGKVIL 720

Query: 721 PELNRLRTIAEDYIHPTLMGDISSPTRSSSQVSLPL 727
           PELNRLR IAED +HPTLMGD +SP+RSSSQVS  L
Sbjct: 721 PELNRLRAIAEDTMHPTLMGD-TSPSRSSSQVSFHL 755

BLAST of Carg21184-RA vs. TrEMBL
Match: tr|A0A2N9FL44|A0A2N9FL44_FAGSY (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS15697 PE=4 SV=1)

HSP 1 Score: 969.5 bits (2505), Expect = 4.1e-279
Identity = 511/777 (65.77%), Postives = 604/777 (77.73%), Query Frame = 0

Query: 1   MWLPRYSLDRKERLNVNGFVAVAIDKDKGSQGALKWAIDHLLRRGQTVFLVHVKLRS--- 60
           MWL R  +++++    NG VAVAID+DKGSQ ALKWAIDHL+++GQT+ L+HV L+S   
Sbjct: 1   MWLARNHVEKRD--EGNGLVAVAIDRDKGSQHALKWAIDHLVQKGQTIILIHVNLKSPLL 60

Query: 61  ---------------LSNSSLKMKSDPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIA 120
                          +S+ S  +  DP+   +ELFLPFRCFCTRKDI CKD++LEDTD+A
Sbjct: 61  STSASLPTPRYHMAGISDVSSLICRDPDQHTKELFLPFRCFCTRKDIHCKDIILEDTDVA 120

Query: 121 KALTEYAANTAIENIVVGASSKSGFL-RFKATDVPGNVSKGAPDFCNVFVISKGKIQSMR 180
           KAL EY +++ IEN++VGAS+K+GFL RFKATDVPG+++KGAPDFCNV+VISKGKIQ+MR
Sbjct: 121 KALIEYVSHSGIENLIVGASAKTGFLRRFKATDVPGSIAKGAPDFCNVYVISKGKIQTMR 180

Query: 181 SASRPAPVVNPLQSQLVQQSSTKSIRSD---YQFP--QTTSARASLDKRPFVLPRRSQDE 240
           SASRPAP V+PL+SQL+ QSS KS   +   Y  P  +     +  +K P    RR QDE
Sbjct: 181 SASRPAPPVSPLRSQLLNQSSIKSDSWEPHLYHAPTMKVMDRVSGTEKPPLEPSRRMQDE 240

Query: 241 ADFM---------------------------SSGRPSIDRIFPSVYDSLDMGGRATPPRM 300
            +FM                           SSGRPSIDR+FPS YD+LD+G   TP RM
Sbjct: 241 TEFMRSPFTRKGYNGKSYGEIPMPDTDISYVSSGRPSIDRMFPSFYDNLDIGD--TPSRM 300

Query: 301 SSSTDLDLTQSFESLPMGRLSMDMNFPSEFSSISQDSDRLSISSQSLEDVEAEMRRLKLE 360
           S S+++D+ QSF+++  GR S+D+  P++FS+IS++ DRLS +SQS+E+VE EMRRLKLE
Sbjct: 301 SYSSEIDMNQSFDNMQPGRNSLDIGSPTDFSTISEEGDRLSNASQSMEEVETEMRRLKLE 360

Query: 361 LKQTMEMYSTACKEALTAKQKAVELQRWKLEEEQRIEEARLAGEAALALAEQEKAKXXXX 420
           LKQT+EMYSTA +EALTAKQ+AVELQRWKLEEE+R++EARLA E ALA+AE+EKAK    
Sbjct: 361 LKQTIEMYSTARREALTAKQRAVELQRWKLEEERRLQEARLAEETALAIAEKEKAKSRAA 420

Query: 421 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKKRAIDALSQSDIRYRKYTIEEIEEATEF 480
             XXXX                         K++A+DAL+QSDIRYR+Y+IEEIE ATEF
Sbjct: 421 IEXXXXAQRIAELESQKRISAELKALKESEEKRKALDALAQSDIRYRRYSIEEIEAATEF 480

Query: 481 FAESRKIGEGGYGPVYKCDLDHTPVAIKVLRPDATQGRSQFKQEVEVLSCIRHPNMVLLL 540
           F +SRKIGEGGYGPVYKC LDHTPVAIKVLRPDA  GRSQF+QEVEVLSCIRHPNMVLLL
Sbjct: 481 FVDSRKIGEGGYGPVYKCYLDHTPVAIKVLRPDAAHGRSQFQQEVEVLSCIRHPNMVLLL 540

Query: 541 GACPEYGCLVYEFMANGSLEDRLLQRGNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLV 600
           GACPEYGCLVYEFMANGSLEDRL  RGNT PLSWQLRF+IA EIGT LLFLHQTKPEPLV
Sbjct: 541 GACPEYGCLVYEFMANGSLEDRLFCRGNTAPLSWQLRFRIASEIGTGLLFLHQTKPEPLV 600

Query: 601 HRDLKPANILLDRNFVSKISDVGLARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGM 660
           HRDLKPANILLDRNFVSKISDVGLARLVPPSVAD VTQYRMTSTAGTFCYIDPEYQQTGM
Sbjct: 601 HRDLKPANILLDRNFVSKISDVGLARLVPPSVADTVTQYRMTSTAGTFCYIDPEYQQTGM 660

Query: 661 LGVKSDIYSLGIMFLQLITSRPPMGLTHHVERAIENDMFANILDPAVTDWPVEEALSFAK 720
           LGVKSDIYSLGIMFLQLIT++PPMGLTHHVERAIE   F  +LDPAV+DWPVEEAL FA+
Sbjct: 661 LGVKSDIYSLGIMFLQLITAKPPMGLTHHVERAIEKGTFHQMLDPAVSDWPVEEALCFAR 720

Query: 721 MSLKCAELRRKDRPDLGKEILPELNRLRTIAEDYIHPTLMGDISSPTRSSSQVSLPL 727
           ++LKC+ELRRKDRPDLGK+++PELNRLR +AE+ +HPT+M      T + SQVSL L
Sbjct: 721 LALKCSELRRKDRPDLGKDVMPELNRLRVLAEETMHPTMMHGSPDLTPAHSQVSLQL 773

BLAST of Carg21184-RA vs. TrEMBL
Match: tr|M5XXG1|M5XXG1_PRUPE (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_ppa001920mg PE=4 SV=1)

HSP 1 Score: 949.1 bits (2452), Expect = 5.7e-273
Identity = 494/734 (67.30%), Postives = 578/734 (78.75%), Query Frame = 0

Query: 1   MWLPRYSLDRKERLNVNGFVAVAIDKDKGSQGALKWAIDHLLRRGQTVFLVHVKLRS--L 60
           MWLP   +D+KE    NG VA+AIDKDK SQ ALKW ID L+++GQTV L+HVK++    
Sbjct: 1   MWLPNNHVDKKE--GGNGIVALAIDKDKNSQSALKWCIDSLVKQGQTVLLIHVKIKPPVF 60

Query: 61  SNSSLKMKSDPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIV 120
           S S      +P+   +ELFLPFR +CTRK+IQCKD+VLED+DIAKAL EY   +AIE++V
Sbjct: 61  SQSPSLSTPNPDPQTKELFLPFRVYCTRKNIQCKDIVLEDSDIAKALNEYVTQSAIEHLV 120

Query: 121 VGASSKSGFLRFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVNPLQSQLV 180
           VGAS+K+GFLRFK +DVPGNVSKGAPDFCNV+V+SKGK+QS RSASRPAP ++P+++ + 
Sbjct: 121 VGASAKTGFLRFKISDVPGNVSKGAPDFCNVYVVSKGKLQSRRSASRPAPSISPIRAPVR 180

Query: 181 QQSSTKSIRSDY----QFPQTTSARASLDKRPFVLPRRSQDEADFMSSGRPSIDRIFPSV 240
            +   +  R  +     F    + +    K    LP    D   ++SSGRPSIDRIFP+ 
Sbjct: 181 HEKPPEPHRKSHDEMENFRSPFTRKGPNGKSYADLPMPDTD-ISYVSSGRPSIDRIFPAF 240

Query: 241 YDSLDMGGRATPPRMSSSTDLDLTQSFESLP-MGRLSMDM-NFPSEFSSISQDSDRLSIS 300
           YD+LD  GR TPPRMSSST++DL  SFES+   GR S+D+ + P  FSS+SQDSDRLS +
Sbjct: 241 YDNLD-SGRITPPRMSSSTEIDLNHSFESMQYFGRRSVDVSSSPPTFSSVSQDSDRLSSA 300

Query: 301 SQSLEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKAVELQRWKLEEEQRIEEARLAG 360
           SQ +EDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKAVELQRWKLEEE+R+EEA LA 
Sbjct: 301 SQPMEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKAVELQRWKLEEERRLEEAHLAE 360

Query: 361 EAALALAEQEKAKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKKRAIDALSQSD 420
           EAA+A+ E+E+AK                                   K++A++AL  SD
Sbjct: 361 EAAMAIVERERAKSRAAIEAAEAAKRIAELEAQKRINAEMKALKESEEKRKALNALQHSD 420

Query: 421 IRYRKYTIEEIEEATEFFAESRKIGEGGYGPVYKCDLDHTPVAIKVLRPDATQGRSQFKQ 480
           +RYRKYTI+EIE ATEFF ESRKIGEGGYGPVY+C LDHTPVAIKVLRPDA QGRSQF Q
Sbjct: 421 VRYRKYTIDEIEAATEFFTESRKIGEGGYGPVYRCYLDHTPVAIKVLRPDAAQGRSQFNQ 480

Query: 481 EVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDRLLQRGNTPPLSWQLRFQIAGE 540
           EVEVLS IRHPNMVLLLGACPEYGCLVYE+MANGSLED LL+RGN+PPLSWQLRF+IA E
Sbjct: 481 EVEVLSSIRHPNMVLLLGACPEYGCLVYEYMANGSLEDCLLRRGNSPPLSWQLRFRIAAE 540

Query: 541 IGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDVGLARLVPPSVADNVTQYRMTS 600
           IGT LLFLHQ KPEPLVHRDLKP NIL+DRNFVSKISDVGLARLVPP+VADNVTQYRMTS
Sbjct: 541 IGTGLLFLHQAKPEPLVHRDLKPGNILIDRNFVSKISDVGLARLVPPAVADNVTQYRMTS 600

Query: 601 TAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRPPMGLTHHVERAIENDMFANIL 660
           TAGTFCYIDPEYQQTGMLGVKSD+YSLGIMFLQ+IT++P MGLTHHVERAIE   F ++L
Sbjct: 601 TAGTFCYIDPEYQQTGMLGVKSDVYSLGIMFLQMITAKPAMGLTHHVERAIEKGTFEDML 660

Query: 661 DPAVTDWPVEEALSFAKMSLKCAELRRKDRPDLGKEILPELNRLRTIAEDYIHPTLMGDI 720
           DP+V DWPV+EA+ FAK++L+C+ELRRKDRPDLGK ILPELNRLR IAED +H TLM   
Sbjct: 661 DPSVPDWPVKEAMKFAKLALQCSELRRKDRPDLGKVILPELNRLRGIAEDTMHHTLMAG- 720

Query: 721 SSPTRSSSQVSLPL 727
            SP+   SQVS+ L
Sbjct: 721 DSPSSQHSQVSMQL 729

BLAST of Carg21184-RA vs. TrEMBL
Match: tr|A0A251RD95|A0A251RD95_PRUPE (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_1G457100 PE=4 SV=1)

HSP 1 Score: 943.7 bits (2438), Expect = 2.4e-271
Identity = 503/771 (65.24%), Postives = 588/771 (76.26%), Query Frame = 0

Query: 1   MWLPRYSLDRKERLNVNGFVAVAIDKDKGSQGALKWAIDHLLRRGQTVFLVHVKLR---- 60
           MWLP   +D+KE    NG VA+AIDKDK SQ ALKW ID L+++GQTV L+HVK++    
Sbjct: 1   MWLPNNHVDKKE--GGNGIVALAIDKDKNSQSALKWCIDSLVKQGQTVLLIHVKIKPPVF 60

Query: 61  SLSNSSLKMKS-----------DPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKAL 120
           S S S    +S           DP+   +ELFLPFR +CTRK+IQCKD+VLED+DIAKAL
Sbjct: 61  SQSPSLSTPRSNPNWDQHLVCKDPDPQTKELFLPFRVYCTRKNIQCKDIVLEDSDIAKAL 120

Query: 121 TEYAANTAIENIVVGASSKSGFLRFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASR 180
            EY   +AIE++VVGAS+K+GFLRFK +DVPGNVSKGAPDFCNV+V+SKGK+QS RSASR
Sbjct: 121 NEYVTQSAIEHLVVGASAKTGFLRFKISDVPGNVSKGAPDFCNVYVVSKGKLQSRRSASR 180

Query: 181 PAPVVNPLQSQLVQQSSTKSIRSD-YQFPQTTSARASLDKRPFVLPRRSQDEAD------ 240
           PAP ++P+++ +  +S T S +S+ Y FP +          P    R+S DE +      
Sbjct: 181 PAPSISPIRAPV--RSHTPSAKSEPYVFPPSGMKGHEKPPEPH---RKSHDEMENFRSPF 240

Query: 241 ---------------------FMSSGRPSIDRIFPSVYDSLDMGGRATPPRMSSSTDLDL 300
                                ++SSGRPSIDRIFP+ YD+LD  GR TPPRMSSST++DL
Sbjct: 241 TRKGPNGKSYADLPMPDTDISYVSSGRPSIDRIFPAFYDNLD-SGRITPPRMSSSTEIDL 300

Query: 301 TQSFESLP-MGRLSMDM-NFPSEFSSISQDSDRLSISSQSLEDVEAEMRRLKLELKQTME 360
             SFES+   GR S+D+ + P  FSS+SQDSDRLS +SQ +EDVEAEMRRLKLELKQTME
Sbjct: 301 NHSFESMQYFGRRSVDVSSSPPTFSSVSQDSDRLSSASQPMEDVEAEMRRLKLELKQTME 360

Query: 361 MYSTACKEALTAKQKAVELQRWKLEEEQRIEEARLAGEAALALAEQEKAKXXXXXXXXXX 420
           MYSTACKEALTAKQKAVELQRWKLEEE+R+EEA LA EAA+A+ E+E+AK          
Sbjct: 361 MYSTACKEALTAKQKAVELQRWKLEEERRLEEAHLAEEAAMAIVERERAKSRAAIEAAEA 420

Query: 421 XXXXXXXXXXXXXXXXXXXXXXXXXKKRAIDALSQSDIRYRKYTIEEIEEATEFFAESRK 480
                                    K++A++AL  SD+RYRKYTI+EIE ATEFF ESRK
Sbjct: 421 AKRIAELEAQKRINAEMKALKESEEKRKALNALQHSDVRYRKYTIDEIEAATEFFTESRK 480

Query: 481 IGEGGYGPVYKCDLDHTPVAIKVLRPDATQGRSQFKQEVEVLSCIRHPNMVLLLGACPEY 540
           IGEGGYGPVY+C LDHTPVAIKVLRPDA QGRSQF QEVEVLS IRHPNMVLLLGACPEY
Sbjct: 481 IGEGGYGPVYRCYLDHTPVAIKVLRPDAAQGRSQFNQEVEVLSSIRHPNMVLLLGACPEY 540

Query: 541 GCLVYEFMANGSLEDRLLQRGNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKP 600
           GCLVYE+MANGSLED LL+RGN+PPLSWQLRF+IA EIGT LLFLHQ KPEPLVHRDLKP
Sbjct: 541 GCLVYEYMANGSLEDCLLRRGNSPPLSWQLRFRIAAEIGTGLLFLHQAKPEPLVHRDLKP 600

Query: 601 ANILLDRNFVSKISDVGLARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSD 660
            NIL+DRNFVSKISDVGLARLVPP+VADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSD
Sbjct: 601 GNILIDRNFVSKISDVGLARLVPPAVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSD 660

Query: 661 IYSLGIMFLQLITSRPPMGLTHHVERAIENDMFANILDPAVTDWPVEEALSFAKMSLKCA 720
           +YSLGIMFLQ+IT++P MGLTHHVERAIE   F ++LDP+V DWPV+EA+ FAK++L+C+
Sbjct: 661 VYSLGIMFLQMITAKPAMGLTHHVERAIEKGTFEDMLDPSVPDWPVKEAMKFAKLALQCS 720

Query: 721 ELRRKDRPDLGKEILPELNRLRTIAEDYIHPTLMGDISSPTRSSSQVSLPL 727
           ELRRKDRPDLGK ILPELNRLR IAED +H TLM    SP+   SQVS+ L
Sbjct: 721 ELRRKDRPDLGKVILPELNRLRGIAEDTMHHTLMAG-DSPSSQHSQVSMQL 762

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022941502.10.0e+0095.62U-box domain-containing protein 52-like isoform X2 [Cucurbita moschata][more]
XP_022941501.10.0e+0095.50U-box domain-containing protein 52-like isoform X1 [Cucurbita moschata][more]
XP_023524077.10.0e+0092.84U-box domain-containing protein 52-like isoform X2 [Cucurbita pepo subsp. pepo][more]
XP_023524076.10.0e+0092.72U-box domain-containing protein 52-like isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_022982091.10.0e+0094.03U-box domain-containing protein 35-like isoform X2 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT4G31230.11.5e-24261.02Protein kinase protein with adenine nucleotide alpha hydrolases-like domain[more]
AT2G24370.13.8e-23859.72Protein kinase protein with adenine nucleotide alpha hydrolases-like domain[more]
AT1G16760.18.2e-20953.36Protein kinase protein with adenine nucleotide alpha hydrolases-like domain[more]
AT1G78940.22.6e-20755.38Protein kinase protein with adenine nucleotide alpha hydrolases-like domain[more]
AT5G35380.17.2e-20556.80Protein kinase protein with adenine nucleotide alpha hydrolases-like domain[more]
Match NameE-valueIdentityDescription
sp|Q9FKG5|PUB51_ARATH5.0e-10742.28U-box domain-containing protein 51 OS=Arabidopsis thaliana OX=3702 GN=PUB51 PE=2... [more]
sp|Q9FKG6|PUB52_ARATH6.8e-10436.24U-box domain-containing protein 52 OS=Arabidopsis thaliana OX=3702 GN=PUB52 PE=2... [more]
sp|Q8S8S7|PUB34_ARATH8.9e-10436.63U-box domain-containing protein 34 OS=Arabidopsis thaliana OX=3702 GN=PUB34 PE=3... [more]
sp|Q9SW11|PUB35_ARATH2.6e-10340.41U-box domain-containing protein 35 OS=Arabidopsis thaliana OX=3702 GN=PUB35 PE=2... [more]
sp|Q9LU47|PUB53_ARATH2.0e-8752.04Putative U-box domain-containing protein 53 OS=Arabidopsis thaliana OX=3702 GN=P... [more]
Match NameE-valueIdentityDescription
tr|A0A1S3CQW0|A0A1S3CQW0_CUCME0.0e+0084.37U-box domain-containing protein 52 OS=Cucumis melo OX=3656 GN=LOC103503739 PE=4 ... [more]
tr|A0A0A0LHG8|A0A0A0LHG8_CUCSA0.0e+0083.07Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G882930 PE=4 SV=1[more]
tr|A0A2N9FL44|A0A2N9FL44_FAGSY4.1e-27965.77Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS15697 PE=4 SV=1[more]
tr|M5XXG1|M5XXG1_PRUPE5.7e-27367.30Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_ppa001920mg PE=4 SV=1[more]
tr|A0A251RD95|A0A251RD95_PRUPE2.4e-27165.24Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_1G457100 PE=4 SV=1[more]
The following terms have been associated with this mRNA:
Vocabulary: Biological Process
TermDefinition
GO:0006950response to stress
GO:0006468protein phosphorylation
Vocabulary: Molecular Function
TermDefinition
GO:0004672protein kinase activity
GO:0005524ATP binding
Vocabulary: INTERPRO
TermDefinition
IPR011009Kinase-like_dom_sf
IPR008271Ser/Thr_kinase_AS
IPR006016UspA
IPR014729Rossmann-like_a/b/a_fold
IPR000719Prot_kinase_dom
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
biological_process GO:0006950 response to stress
cellular_component GO:0005575 cellular_component
molecular_function GO:0005524 ATP binding
molecular_function GO:0004672 protein kinase activity

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Carg21184Carg21184gene


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg21184-RA.CDS.9Carg21184-RA.CDS.9CDS
Carg21184-RA.CDS.8Carg21184-RA.CDS.8CDS
Carg21184-RA.CDS.7Carg21184-RA.CDS.7CDS
Carg21184-RA.CDS.6Carg21184-RA.CDS.6CDS
Carg21184-RA.CDS.5Carg21184-RA.CDS.5CDS
Carg21184-RA.CDS.4Carg21184-RA.CDS.4CDS
Carg21184-RA.CDS.3Carg21184-RA.CDS.3CDS
Carg21184-RA.CDS.2Carg21184-RA.CDS.2CDS
Carg21184-RA.CDS.1Carg21184-RA.CDS.1CDS


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg21184-RA.exon.9Carg21184-RA.exon.9exon
Carg21184-RA.exon.8Carg21184-RA.exon.8exon
Carg21184-RA.exon.7Carg21184-RA.exon.7exon
Carg21184-RA.exon.6Carg21184-RA.exon.6exon
Carg21184-RA.exon.5Carg21184-RA.exon.5exon
Carg21184-RA.exon.4Carg21184-RA.exon.4exon
Carg21184-RA.exon.3Carg21184-RA.exon.3exon
Carg21184-RA.exon.2Carg21184-RA.exon.2exon
Carg21184-RA.exon.1Carg21184-RA.exon.1exon


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Carg21184-RACarg21184-RA-proteinpolypeptide


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 289..316
NoneNo IPR availableCOILSCoilCoilcoord: 322..342
NoneNo IPR availableCOILSCoilCoilcoord: 356..402
NoneNo IPR availableGENE3DG3DSA:1.10.510.10coord: 503..705
e-value: 1.5E-45
score: 157.0
NoneNo IPR availableGENE3DG3DSA:3.30.200.20coord: 410..502
e-value: 4.5E-24
score: 86.4
NoneNo IPR availablePANTHERPTHR27003FAMILY NOT NAMEDcoord: 12..711
NoneNo IPR availablePANTHERPTHR27003:SF145ADENINE NUCLEOTIDE ALPHA HYDROLASE DOMAIN-CONTAINING PROTEIN KINASE-RELATEDcoord: 12..711
NoneNo IPR availableCDDcd01989STK_Ncoord: 20..155
e-value: 1.72244E-58
score: 194.802
NoneNo IPR availableSUPERFAMILYSSF52402Adenine nucleotide alpha hydrolases-likecoord: 20..152
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 430..690
e-value: 2.5E-26
score: 103.5
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 431..683
e-value: 1.0E-39
score: 136.4
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 430..708
score: 35.001
IPR014729Rossmann-like alpha/beta/alpha sandwich foldGENE3DG3DSA:3.40.50.620coord: 20..154
e-value: 1.4E-11
score: 46.9
IPR006016UspAPFAMPF00582Uspcoord: 21..144
e-value: 7.9E-11
score: 42.5
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 548..560
IPR011009Protein kinase-like domain superfamilySUPERFAMILYSSF56112Protein kinase-like (PK-like)coord: 409..685