Carg20538-RA (mRNA) Silver-seed gourd

NameCarg20538-RA
TypemRNA
OrganismCucurbita argyrosperma (Silver-seed gourd)
Description5'-adenylylsulfate reductase-like 4
LocationCucurbita_argyrosperma_scaffold_113 : 152068 .. 154080 (+)
Sequence length1000
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
CCTTCTCCATTTCTCTAATTTTGTGATTATTATGAGGATCGGGTTCTGCGTGAGGGGAATTGTTCTGTTGATCGTGATGCTGGGAAGGCAAGCGTCGTCCGCCGACTCGGTTAGGGTTTTGCATTCAGGCCTCTCGTGTCCTATTGAATCTTTTCTGGATACGATCTTTCGTTTTAGGGATTGGAACTCGTACTGCCCCATTGACGGACAGCATGGACACTATGAATTCATTGGCGTTTCTGAGGTAAATTTCTTATTATTTTTCCATTTCTGTTTCTATGATTCATCTGAAATTGTTTGTCCTTAAGATCCTTGACGTTTGAGGGTCTCTTTTGTTTAATTTTGGTCCAAGTTTTGTGGTCAATTTCAGCTTTTTCAGAATATTGAGACATGTTTTCTGGCTCTGTAGTCAATTTTGTTGATGTTAATTTGTTTGAGAGCCCCCTTAACTGTTTAAGGGGTTGAATTCTACGGATCCTAAGCATTTTAGGCTCTGTTTTTGAGTTCATTTCTTTTATCTTTGTTGTTTTGCCACTAAAAGAATTGGGAATTCTGATCTTTACATGAATTTGGGTATAGGAGGCTGTTATAATTGTGTGGGTAACTTAACTATTTGTTTTTGCTATGATTTAAGGGAGATGAGGCCTCCTTACAAATGGCACTGAATATGGTACATAAGAACCGCTATGAATATGTTGCAGTGCTCTTCTATGCATCTTGGTGCCAGTTCTCCAAGTCTTTTAGGCCAAGCTTTTCGATACTATCGTCTTTATACCCTTCGATCCCACATTTTGCAATCCAAGAATCAGCTGTTAGGCCAAGGTTTCATATTGAACTTTCACTTTTTCAATTTGGCATTCTATGGTCGTATCATGTTTACTTTATGTGAACATAATTCATGTTTTCTTGCAGCATACTCTCTAAGTATGGGGTCCATGGTTTTCCTACCCTTTTCCTTTTAAATTCTACAATGCGTGCTCGCTACTACGGTTCTCGAACGCTCCCTTCTCTTGCTGCATTCTATAATGATGTTACTGGTATGGTCTCTTTATGCTTCCTCTAAATTTTGCTTCCATGAAAGGAATACTTGTTTTGAATTCTTAAGAAATGAATTGTGTAGCATTTTTTTGCCCAAGAGATATTCAAATTGCTCAAAATACATGTTGCTACTATTCATTTTAAGTTGCTGATAATATTTTCCATCTTGTATGTAATTCAATTAAAGCGTTGAATCTTGATTCTTGAACATCTTCTCCTTGCATAATCGATTCGTAATGGAGTTTCGTGTTTTTTAGTTAGTCATGTAATAGGTTTTTTTTCTGAGTCATTATTTGTTTTAGTCGTGTGGTGTTTCGAGGCCCCCTTCATCGTAGGACATCGACATATTGGATGAACCAGGGAAGCTTTAACAAGTAGCTATGAACCCCGACGAATAGTGCAAGTGGTTGAAGATATTAATGTTGGTTTAATATGCAGTAATAGTATTTGAATATGTGATGAAGAATCTTAACTGCTATATTGTTAGAACTTAGTTCTATTATTTGATATTGGTGGTCATGGTTGATTATAGGCATTCAAACTGCATCACTGGACCAAATATCGCCAGATAGAATCGAGCAAGTATGGAATCAGGAGAAGCATGATGATAGCAGTGAGCAGGAAAATTGCCCATTCTCATGGGCAAGGTCTCCCGAAAATCTACTTCGGGAAGAAACGTATTTGGCTCTAGCTACTGCGTTCGTGTGTATGAGATTGATATACATCTTCTTTCCAACTCTTTTGGTCTATGCTCAATATGTTTGGCAAAGGCACCTTAGGAACCTGAGATTGGGAACCTTGTGGGAACGACCTTTGACGTGCCTGAAAGGAGCAGTTCAGTTATTTAGCCATTTCAAGGATCCTTGCAAGAGAAGCAATTTACAAGGAGGTGCCATGAATGCCAGGGCATGGGCCAAGTCTTTTGCCACAGTTTCTATAGGCGATGCAAGCTCTAGTAGCCGAGTGTGCCAGTAA

mRNA sequence

CCTTCTCCATTTCTCTAATTTTGTGATTATTATGAGGATCGGGTTCTGCGTGAGGGGAATTGTTCTGTTGATCGTGATGCTGGGAAGGCAAGCGTCGTCCGCCGACTCGGTTAGGGTTTTGCATTCAGGCCTCTCGTGTCCTATTGAATCTTTTCTGGATACGATCTTTCGTTTTAGGGATTGGAACTCGTACTGCCCCATTGACGGACAGCATGGACACTATGAATTCATTGGCGTTTCTGAGGGAGATGAGGCCTCCTTACAAATGGCACTGAATATGGTACATAAGAACCGCTATGAATATGTTGCAGTGCTCTTCTATGCATCTTGGTGCCAGTTCTCCAAGTCTTTTAGGCCAAGCTTTTCGATACTATCGTCTTTATACCCTTCGATCCCACATTTTGCAATCCAAGAATCAGCTGTTAGGCCAAGCATACTCTCTAAGTATGGGGTCCATGGTTTTCCTACCCTTTTCCTTTTAAATTCTACAATGCGTGCTCGCTACTACGGTTCTCGAACGCTCCCTTCTCTTGCTGCATTCTATAATGATGTTACTGGCATTCAAACTGCATCACTGGACCAAATATCGCCAGATAGAATCGAGCAAGTATGGAATCAGGAGAAGCATGATGATAGCAGTGAGCAGGAAAATTGCCCATTCTCATGGGCAAGGTCTCCCGAAAATCTACTTCGGGAAGAAACGTATTTGGCTCTAGCTACTGCGTTCGTGTGTATGAGATTGATATACATCTTCTTTCCAACTCTTTTGGTCTATGCTCAATATGTTTGGCAAAGGCACCTTAGGAACCTGAGATTGGGAACCTTGTGGGAACGACCTTTGACGTGCCTGAAAGGAGCAGTTCAGTTATTTAGCCATTTCAAGGATCCTTGCAAGAGAAGCAATTTACAAGGAGGTGCCATGAATGCCAGGGCATGGGCCAAGTCTTTTGCCACAGTTTCTATAGGCGATGCAAGCTCTAGTAGCCGAGTGTGCCAGTAA

Coding sequence (CDS)

ATGAGGATCGGGTTCTGCGTGAGGGGAATTGTTCTGTTGATCGTGATGCTGGGAAGGCAAGCGTCGTCCGCCGACTCGGTTAGGGTTTTGCATTCAGGCCTCTCGTGTCCTATTGAATCTTTTCTGGATACGATCTTTCGTTTTAGGGATTGGAACTCGTACTGCCCCATTGACGGACAGCATGGACACTATGAATTCATTGGCGTTTCTGAGGGAGATGAGGCCTCCTTACAAATGGCACTGAATATGGTACATAAGAACCGCTATGAATATGTTGCAGTGCTCTTCTATGCATCTTGGTGCCAGTTCTCCAAGTCTTTTAGGCCAAGCTTTTCGATACTATCGTCTTTATACCCTTCGATCCCACATTTTGCAATCCAAGAATCAGCTGTTAGGCCAAGCATACTCTCTAAGTATGGGGTCCATGGTTTTCCTACCCTTTTCCTTTTAAATTCTACAATGCGTGCTCGCTACTACGGTTCTCGAACGCTCCCTTCTCTTGCTGCATTCTATAATGATGTTACTGGCATTCAAACTGCATCACTGGACCAAATATCGCCAGATAGAATCGAGCAAGTATGGAATCAGGAGAAGCATGATGATAGCAGTGAGCAGGAAAATTGCCCATTCTCATGGGCAAGGTCTCCCGAAAATCTACTTCGGGAAGAAACGTATTTGGCTCTAGCTACTGCGTTCGTGTGTATGAGATTGATATACATCTTCTTTCCAACTCTTTTGGTCTATGCTCAATATGTTTGGCAAAGGCACCTTAGGAACCTGAGATTGGGAACCTTGTGGGAACGACCTTTGACGTGCCTGAAAGGAGCAGTTCAGTTATTTAGCCATTTCAAGGATCCTTGCAAGAGAAGCAATTTACAAGGAGGTGCCATGAATGCCAGGGCATGGGCCAAGTCTTTTGCCACAGTTTCTATAGGCGATGCAAGCTCTAGTAGCCGAGTGTGCCAGTAA

Protein sequence

MRIGFCVRGIVLLIVMLGRQASSADSVRVLHSGLSCPIESFLDTIFRFRDWNSYCPIDGQHGHYEFIGVSEGDEASLQMALNMVHKNRYEYVAVLFYASWCQFSKSFRPSFSILSSLYPSIPHFAIQESAVRPSILSKYGVHGFPTLFLLNSTMRARYYGSRTLPSLAAFYNDVTGIQTASLDQISPDRIEQVWNQEKHDDSSEQENCPFSWARSPENLLREETYLALATAFVCMRLIYIFFPTLLVYAQYVWQRHLRNLRLGTLWERPLTCLKGAVQLFSHFKDPCKRSNLQGGAMNARAWAKSFATVSIGDASSSSRVCQ
BLAST of Carg20538-RA vs. NCBI nr
Match: XP_022952011.1 (5'-adenylylsulfate reductase-like 4 [Cucurbita moschata] >XP_023538087.1 5'-adenylylsulfate reductase-like 4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 647.5 bits (1669), Expect = 2.4e-182
Identity = 321/322 (99.69%), Postives = 321/322 (99.69%), Query Frame = 0

Query: 1   MRIGFCVRGIVLLIVMLGRQASSADSVRVLHSGLSCPIESFLDTIFRFRDWNSYCPIDGQ 60
           MRIGFCVRGIVLLIVMLGRQASSADSVRVLHSGLSCPIESFLDTIFRFRDWNSYCPIDGQ
Sbjct: 1   MRIGFCVRGIVLLIVMLGRQASSADSVRVLHSGLSCPIESFLDTIFRFRDWNSYCPIDGQ 60

Query: 61  HGHYEFIGVSEGDEASLQMALNMVHKNRYEYVAVLFYASWCQFSKSFRPSFSILSSLYPS 120
           HGHYEFIGVSEGDEASLQMALNMVHKNRYEYVAVLFYASWCQFSKSFRPSFSILSSLYPS
Sbjct: 61  HGHYEFIGVSEGDEASLQMALNMVHKNRYEYVAVLFYASWCQFSKSFRPSFSILSSLYPS 120

Query: 121 IPHFAIQESAVRPSILSKYGVHGFPTLFLLNSTMRARYYGSRTLPSLAAFYNDVTGIQTA 180
           IPHFAIQESAVRPSILSKYGVHGFPTLFLLNSTMRARYYGSRTLPSLAAFYNDVTGIQTA
Sbjct: 121 IPHFAIQESAVRPSILSKYGVHGFPTLFLLNSTMRARYYGSRTLPSLAAFYNDVTGIQTA 180

Query: 181 SLDQISPDRIEQVWNQEKHDDSSEQENCPFSWARSPENLLREETYLALATAFVCMRLIYI 240
           SLDQISPDRIEQVWNQEKHDDSSEQENCPFSWARSPENLLREETYLALATAFV MRLIYI
Sbjct: 181 SLDQISPDRIEQVWNQEKHDDSSEQENCPFSWARSPENLLREETYLALATAFVFMRLIYI 240

Query: 241 FFPTLLVYAQYVWQRHLRNLRLGTLWERPLTCLKGAVQLFSHFKDPCKRSNLQGGAMNAR 300
           FFPTLLVYAQYVWQRHLRNLRLGTLWERPLTCLKGAVQLFSHFKDPCKRSNLQGGAMNAR
Sbjct: 241 FFPTLLVYAQYVWQRHLRNLRLGTLWERPLTCLKGAVQLFSHFKDPCKRSNLQGGAMNAR 300

Query: 301 AWAKSFATVSIGDASSSSRVCQ 323
           AWAKSFATVSIGDASSSSRVCQ
Sbjct: 301 AWAKSFATVSIGDASSSSRVCQ 322

BLAST of Carg20538-RA vs. NCBI nr
Match: XP_023002667.1 (5'-adenylylsulfate reductase-like 4 [Cucurbita maxima])

HSP 1 Score: 642.5 bits (1656), Expect = 7.6e-181
Identity = 318/322 (98.76%), Postives = 319/322 (99.07%), Query Frame = 0

Query: 1   MRIGFCVRGIVLLIVMLGRQASSADSVRVLHSGLSCPIESFLDTIFRFRDWNSYCPIDGQ 60
           MRIGFCVRGIVLLIVMLGRQA SADSVRVLHSGLSCPIESFLDTIFRFRDWNSYCPIDGQ
Sbjct: 1   MRIGFCVRGIVLLIVMLGRQAFSADSVRVLHSGLSCPIESFLDTIFRFRDWNSYCPIDGQ 60

Query: 61  HGHYEFIGVSEGDEASLQMALNMVHKNRYEYVAVLFYASWCQFSKSFRPSFSILSSLYPS 120
           HGHYEFIGVSEGDEASLQMALNMVHKNRYEYVAVLFYASWCQFSKSFRPSFSILSSLYPS
Sbjct: 61  HGHYEFIGVSEGDEASLQMALNMVHKNRYEYVAVLFYASWCQFSKSFRPSFSILSSLYPS 120

Query: 121 IPHFAIQESAVRPSILSKYGVHGFPTLFLLNSTMRARYYGSRTLPSLAAFYNDVTGIQTA 180
           IPHFAIQESAVRPSILSKYGVHGFPTLFLLNSTMRARYYGSRTLPSLAAFYNDVTGIQTA
Sbjct: 121 IPHFAIQESAVRPSILSKYGVHGFPTLFLLNSTMRARYYGSRTLPSLAAFYNDVTGIQTA 180

Query: 181 SLDQISPDRIEQVWNQEKHDDSSEQENCPFSWARSPENLLREETYLALATAFVCMRLIYI 240
           SLDQI PDRIEQVWNQEKHDDSSEQENCPFSWARSPENLLREETYLALATAFV MRL+YI
Sbjct: 181 SLDQILPDRIEQVWNQEKHDDSSEQENCPFSWARSPENLLREETYLALATAFVFMRLVYI 240

Query: 241 FFPTLLVYAQYVWQRHLRNLRLGTLWERPLTCLKGAVQLFSHFKDPCKRSNLQGGAMNAR 300
           FFPTLLVYAQYVWQRHLRNLRLGTLWERPLTCLKGAVQLFSHFKDPCKRSNLQGGAMNAR
Sbjct: 241 FFPTLLVYAQYVWQRHLRNLRLGTLWERPLTCLKGAVQLFSHFKDPCKRSNLQGGAMNAR 300

Query: 301 AWAKSFATVSIGDASSSSRVCQ 323
           AWAKSFATVSIGDASSSSRVCQ
Sbjct: 301 AWAKSFATVSIGDASSSSRVCQ 322

BLAST of Carg20538-RA vs. NCBI nr
Match: XP_022131641.1 (5'-adenylylsulfate reductase-like 4 [Momordica charantia])

HSP 1 Score: 572.0 bits (1473), Expect = 1.3e-159
Identity = 285/322 (88.51%), Postives = 299/322 (92.86%), Query Frame = 0

Query: 1   MRIGFCVRGIVLLIVMLGRQASSADSVRVLHSGLSCPIESFLDTIFRFRDWNSYCPIDGQ 60
           MR GF V GI++L+V+ G Q  SA+SVRV HS  SCP ES LDTIFRFRDWNS CPIDG+
Sbjct: 1   MRTGFWVPGILVLVVLFGGQPCSANSVRVSHSAPSCPAESLLDTIFRFRDWNSNCPIDGE 60

Query: 61  HGHYEFIGVSEGDEASLQMALNMVHKNRYEYVAVLFYASWCQFSKSFRPSFSILSSLYPS 120
            GHYEFIGVSEGDEASLQ+ALNMVHKN YEYVAVLFYASWC FSKSFRPSFSILSSLYPS
Sbjct: 61  DGHYEFIGVSEGDEASLQLALNMVHKNHYEYVAVLFYASWCPFSKSFRPSFSILSSLYPS 120

Query: 121 IPHFAIQESAVRPSILSKYGVHGFPTLFLLNSTMRARYYGSRTLPSLAAFYNDVTGIQTA 180
           IPHFAIQESAVRPSILSKYGVHGFPTLFLLNSTMRARYYGSRTLPSL AFYNDVTGIQTA
Sbjct: 121 IPHFAIQESAVRPSILSKYGVHGFPTLFLLNSTMRARYYGSRTLPSLVAFYNDVTGIQTA 180

Query: 181 SLDQISPDRIEQVWNQEKHDDSSEQENCPFSWARSPENLLREETYLALATAFVCMRLIYI 240
           SL+QISPDRI QVWNQEKH D+SEQENCPFSWARSPENL REETYLALATAFV MRLI+I
Sbjct: 181 SLNQISPDRIGQVWNQEKH-DNSEQENCPFSWARSPENLFREETYLALATAFVLMRLIHI 240

Query: 241 FFPTLLVYAQYVWQRHLRNLRLGTLWERPLTCLKGAVQLFSHFKDPCKRSNLQGGAMNAR 300
           FFPTLLVYA+ +W+RHLRNLRLGTLWERPLTCLKGAVQLFSHFKDPCKR NLQGGAMNAR
Sbjct: 241 FFPTLLVYARDIWRRHLRNLRLGTLWERPLTCLKGAVQLFSHFKDPCKRRNLQGGAMNAR 300

Query: 301 AWAKSFATVSIGDASSSSRVCQ 323
           AWAKSFATVSIGDASSS+RVCQ
Sbjct: 301 AWAKSFATVSIGDASSSTRVCQ 321

BLAST of Carg20538-RA vs. NCBI nr
Match: XP_022997216.1 (5'-adenylylsulfate reductase-like 4 [Cucurbita maxima])

HSP 1 Score: 550.4 bits (1417), Expect = 4.0e-153
Identity = 272/316 (86.08%), Postives = 288/316 (91.14%), Query Frame = 0

Query: 1   MRIGFCVRGIVLLIVMLGRQASSADSVRVLHSGLSCPIESFLDTIFRFRDWNSYCPIDGQ 60
           MR GF V GIV+L +ML  QASSA SVRV HSG SCP+ESFLDTIF F+DWNS CP  G+
Sbjct: 11  MRTGFWVMGIVVLAMMLESQASSAHSVRVSHSGPSCPVESFLDTIFPFKDWNSICPFHGE 70

Query: 61  HGHYEFIGVSEGDEASLQMALNMVHKNRYEYVAVLFYASWCQFSKSFRPSFSILSSLYPS 120
            GH EFIGVSEGDEA LQMA+NMVHKNRY+YVAVLFYASWC FSKSFRP FSILS+LYPS
Sbjct: 71  DGHSEFIGVSEGDEALLQMAMNMVHKNRYDYVAVLFYASWCPFSKSFRPRFSILSTLYPS 130

Query: 121 IPHFAIQESAVRPSILSKYGVHGFPTLFLLNSTMRARYYGSRTLPSLAAFYNDVTGIQTA 180
           IPHFAIQ+SA+RPSILSKYGVHGFPTLFLLNSTMRARYYGSRTLPSL  FYNDVTGIQTA
Sbjct: 131 IPHFAIQDSAIRPSILSKYGVHGFPTLFLLNSTMRARYYGSRTLPSLIGFYNDVTGIQTA 190

Query: 181 SLDQISPDRIEQVWNQEKHDDSSEQENCPFSWARSPENLLREETYLALATAFVCMRLIYI 240
           SLDQISPD I QV N+EKHDD+ EQENCPFSWARSPENLLREETYLALATAFV +RLIYI
Sbjct: 191 SLDQISPDIIGQVRNEEKHDDNYEQENCPFSWARSPENLLREETYLALATAFVLLRLIYI 250

Query: 241 FFPTLLVYAQYVWQRHLRNLRLGTLWERPLTCLKGAVQLFSHFKDPCKRSNLQGGAMNAR 300
            FPTLLVYA+YVWQRH+RNLR GTLWERPLTCLKGA+QLFSHFKDPCKRSNLQGGAMNAR
Sbjct: 251 LFPTLLVYARYVWQRHVRNLRFGTLWERPLTCLKGAIQLFSHFKDPCKRSNLQGGAMNAR 310

Query: 301 AWAKSFATVSIGDASS 317
            WAKSFATVSIGDASS
Sbjct: 311 IWAKSFATVSIGDASS 326

BLAST of Carg20538-RA vs. NCBI nr
Match: XP_023545571.1 (5'-adenylylsulfate reductase-like 4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 543.9 bits (1400), Expect = 3.7e-151
Identity = 267/316 (84.49%), Postives = 287/316 (90.82%), Query Frame = 0

Query: 1   MRIGFCVRGIVLLIVMLGRQASSADSVRVLHSGLSCPIESFLDTIFRFRDWNSYCPIDGQ 60
           MR GF V GIV+L +ML  QASSA SVRV HSG SCP++SFLDTIF  RDWNS CP  G+
Sbjct: 1   MRTGFWVMGIVVLAMMLESQASSAHSVRVSHSGPSCPVQSFLDTIFSSRDWNSNCPFHGE 60

Query: 61  HGHYEFIGVSEGDEASLQMALNMVHKNRYEYVAVLFYASWCQFSKSFRPSFSILSSLYPS 120
            GH+EFIGVSEGDEA LQMA+NM+HKNRY+YVAVLFYASWC FSKSFRP FSILS+LY S
Sbjct: 61  DGHFEFIGVSEGDEALLQMAMNMIHKNRYDYVAVLFYASWCPFSKSFRPRFSILSTLYRS 120

Query: 121 IPHFAIQESAVRPSILSKYGVHGFPTLFLLNSTMRARYYGSRTLPSLAAFYNDVTGIQTA 180
           IPHFAIQ+SA+RPSILSKYGVHGFPTLFLLNSTMRARYYGSRTLPSL  FYNDVTGIQ+A
Sbjct: 121 IPHFAIQDSAIRPSILSKYGVHGFPTLFLLNSTMRARYYGSRTLPSLVGFYNDVTGIQSA 180

Query: 181 SLDQISPDRIEQVWNQEKHDDSSEQENCPFSWARSPENLLREETYLALATAFVCMRLIYI 240
           SLDQISPD I QV N+EKHDD++EQENCPFSWARSPENLLREETYLALAT FV +RLIYI
Sbjct: 181 SLDQISPDIIGQVRNEEKHDDNNEQENCPFSWARSPENLLREETYLALATVFVLLRLIYI 240

Query: 241 FFPTLLVYAQYVWQRHLRNLRLGTLWERPLTCLKGAVQLFSHFKDPCKRSNLQGGAMNAR 300
           FFPTLLVYA+YVWQRH+RNLR GTLWERPLTCLKGA+Q FSHFKDPCKRSNLQGGAMNAR
Sbjct: 241 FFPTLLVYARYVWQRHVRNLRFGTLWERPLTCLKGAIQSFSHFKDPCKRSNLQGGAMNAR 300

Query: 301 AWAKSFATVSIGDASS 317
            WAKSFATVSIGDASS
Sbjct: 301 IWAKSFATVSIGDASS 316

BLAST of Carg20538-RA vs. TAIR10
Match: AT1G34780.1 (APR-like 4)

HSP 1 Score: 317.0 bits (811), Expect = 1.3e-86
Identity = 175/313 (55.91%), Postives = 230/313 (73.48%), Query Frame = 0

Query: 10  IVLLIVMLGRQASSADSVRVLHSGLSCPIESFLDTIFRFRDWNSYCPIDGQHG--HYEFI 69
           ++LL+V++    +  D+VRV      C  +S  D+IF  RD    C + G        F+
Sbjct: 6   LLLLLVIMFLTVADVDAVRVPF----CATKSAKDSIFGLRD--QTCSVSGVESDERPRFV 65

Query: 70  GVSEGDEASLQMALNMVHKNRYEYVAVLFYASWCQFSKSFRPSFSILSSLYPSIPHFAIQ 129
            V+EGDE  LQ+AL+M+HKN+ +YVA+LFYASWC FS+SFRPSF ++SSLY SIPHFAI+
Sbjct: 66  AVTEGDERWLQIALDMIHKNKCDYVALLFYASWCPFSRSFRPSFDVISSLYSSIPHFAIK 125

Query: 130 ESAVRPSILSKYGVHGFPTLFLLNSTMRARYYGSRTLPSLAAFYNDVTGIQTASLDQISP 189
           ES+++PS LSKYGVHGFPTL LLNSTMRARY G+R L SL AFY+DVTGI+T  LD+ S 
Sbjct: 126 ESSIKPSTLSKYGVHGFPTLLLLNSTMRARYRGTRMLDSLVAFYSDVTGIET--LDKTSL 185

Query: 190 DRIEQVWNQEKHDDSSEQENCPFSWARSPENLLREETYLALATAFVCMRLIYIFFPTLLV 249
           +R   V     +++++E ENCPF+WARSPEN+LR+ETYLALA  FV +RL+++ +PTL+V
Sbjct: 186 ERSVSV-PHLGNENNTEPENCPFTWARSPENMLRQETYLALAIVFVLLRLLHLIYPTLVV 245

Query: 250 YAQYVWQRHLRNLRLGTLWERPLTCLKGAVQLFSHFKDPCKRSNLQGGAMNARAWA-KSF 309
           + ++ W+R  +N+RL +L E  +  L  AVQL  H     +RSNLQGGAMNARAWA KS 
Sbjct: 246 FMKFTWRRIAQNMRLESLLEHTVGFLSRAVQLCMH-----RRSNLQGGAMNARAWASKSL 304

Query: 310 ATVSIGDASSSSR 320
           ATVSIGD+SSS+R
Sbjct: 306 ATVSIGDSSSSNR 304

BLAST of Carg20538-RA vs. TAIR10
Match: AT4G08930.1 (APR-like 6)

HSP 1 Score: 256.1 bits (653), Expect = 2.8e-68
Identity = 156/313 (49.84%), Postives = 206/313 (65.81%), Query Frame = 0

Query: 10  IVLLIVMLGRQASSADSVRVLHSGLSCPIESFLDTIFRFRDWNSYCPIDGQHGHYEFIGV 69
           ++L++V+L    ++A +VRV      CP ES  D I  FRD ++          +    V
Sbjct: 8   LLLVVVVLFVNLTNA-TVRV----QICPRESAKDYILGFRDKSAL---------HRPGFV 67

Query: 70  SEGDEASLQMALNMV-HKNRYEYVAVLFYASWCQFSKSFRPSFSILSSLYPSIPHFAIQE 129
           +EGD+  LQMA +MV  KN+ +Y A+LFYASWC FS+  RPSF ++S LY S+PHFAI+E
Sbjct: 68  TEGDDRWLQMAADMVDKKNKCDYAALLFYASWCPFSRLVRPSFDLMSLLYSSVPHFAIEE 127

Query: 130 SAVRPSILSKYGVHGFPTLFLLNSTMRARYYGSRTLPSLAAFYNDVTGIQTASLDQISPD 189
           S+V+ S LSKYGVHGFPT+ L+NSTM   Y GSRTL SL AFY DVTGI+T     +  +
Sbjct: 128 SSVKASTLSKYGVHGFPTIILMNSTMLVVYRGSRTLDSLVAFYTDVTGIETMDERWVERN 187

Query: 190 RIEQVWNQEKHDDSSEQENCPFSWA-RSPENLLREETYLALATAFVCMRLIYIFFPTLLV 249
           R+   ++       +E ENCPF WA RSPENLLR+ETYL LAT FV +RL+++  PT++V
Sbjct: 188 RLVPHFH-------AEPENCPFPWARRSPENLLRQETYLTLATVFVLLRLLHLISPTMVV 247

Query: 250 YAQYVWQRHLRNLRLGTLWERPLTCLKGAVQLFSHFKDPCKRSNLQGGAMNARAWA-KSF 309
           + ++ W R + N+RLG   E  +T          + K+PC  SNLQ GAMNARAWA KS 
Sbjct: 248 FVKFTWGR-VSNMRLGNPLEHTVT---------MYLKEPCMSSNLQEGAMNARAWASKSL 289

Query: 310 ATVSIGDASSSSR 320
           ATVSI ++SSSSR
Sbjct: 308 ATVSIAESSSSSR 289

BLAST of Carg20538-RA vs. TAIR10
Match: AT3G03860.1 (APR-like 5)

HSP 1 Score: 135.6 bits (340), Expect = 5.5e-32
Identity = 87/261 (33.33%), Postives = 133/261 (50.96%), Query Frame = 0

Query: 71  EGDEASLQMALNMVHKNRYEYVAVLFYASWCQFSKSFRPSFSILSSLYPSIPHFAIQESA 130
           E D  SL   +   H N   Y++VLFYASWC FS++ RP F +LSS++P I H A++ S 
Sbjct: 58  EVDGDSLDRLMASQHGN--AYMSVLFYASWCPFSRAVRPKFDMLSSMFPQIQHLAVEHSQ 117

Query: 131 VRPSILSKYGVHGFPTLFLLNSTMRARYYGSRTLPSLAAFYNDVTGIQTASLDQISPDRI 190
             PS+ S+YG+H  P++ ++N T+ ARY+G + L SL  FY + TG+Q           +
Sbjct: 118 ALPSVFSRYGIHSLPSILMVNQTLNARYHGRKDLISLIEFYEEATGLQP----------V 177

Query: 191 EQVWNQEKHDDSSEQENCPFSWAR---SPENLLREETYLALATAFVCMRLIYIFFPTLLV 250
           + V   E    ++   N   +W R   S   + +++ +L L+  F+C+++  + FP    
Sbjct: 178 QYVAEGEPTGLNAGDGNL-ITWLRKGTSIREIFKQDPFLVLSLLFICLQMAILVFPIAES 237

Query: 251 YAQYVWQRHLRNLRLGTLWERPLTCLKGAVQLFS---HFKDP---------CKRSNLQGG 310
             + +W  ++ NL LG   E          QLF+   H  D           K  N    
Sbjct: 238 RMRALWASYVANLNLGRFGE--------ISQLFNRGIHMVDVRRLWLKLSLVKTRNFHER 297

Query: 311 AMNARAWAKSFATVSIGDASS 317
           A NA+AWA S A+VS+G  SS
Sbjct: 298 AKNAQAWASSLASVSLGQTSS 297

BLAST of Carg20538-RA vs. TAIR10
Match: AT5G18120.1 (APR-like 7)

HSP 1 Score: 112.5 bits (280), Expect = 5.0e-25
Identity = 82/263 (31.18%), Postives = 129/263 (49.05%), Query Frame = 0

Query: 71  EGDEASLQMALNMVHKNRYEYVAVLFYASWCQFSKSFRPSFSILSSLYPSIPHFAIQESA 130
           E D   L   ++  H N   Y+++LFY S C FS++ RP F +LSS++P I H  +++S 
Sbjct: 54  EVDGDLLDKLMDANHGN--AYISILFYTSRCPFSRAVRPKFDVLSSMFPHITHLIVEQSQ 113

Query: 131 VRPSILSKYGVHGFPTLFLLNSTMRARYYGSRTLPSLAAFYNDVTGIQTAS-LDQISPDR 190
             PS+ S+YG+H  P++ ++N TM+ RY+G + L SL  FY + TG++    +D+  P  
Sbjct: 114 ALPSVFSRYGIHSLPSILMVNQTMKMRYHGPKDLASLIQFYKETTGLKPVQYMDEGEPTS 173

Query: 191 IEQVWN--QEKHDDSSEQENCPFSWARSPENLLREETYLALATAFVCMRLIYIFFPTLLV 250
           ++   N     H+ SS +E            +   E Y+ LA  F+ ++L  + FP +  
Sbjct: 174 LDTDGNLITWLHNGSSIRE------------IAEREPYMVLALMFLSLKLAILIFPIMGS 233

Query: 251 YAQYVWQRHLRNLRLGTLWERPLTCLKGAVQLFS---HFKDP---------CKRSNLQGG 310
             + +W  ++ +L LG L E          QLF    H  D           K  N Q  
Sbjct: 234 RLKTLWALYVPHLSLGILGE--------TSQLFGRALHMIDVRRLWIKLRLTKTRNFQER 288

Query: 311 AMNARAWAKSFATVSIGDASSSS 319
           A NA       A+VS+G +SS S
Sbjct: 294 AKNA------LASVSLGKSSSQS 288

BLAST of Carg20538-RA vs. TAIR10
Match: AT1G21750.1 (PDI-like 1-1)

HSP 1 Score: 42.0 bits (97), Expect = 8.3e-04
Identity = 33/119 (27.73%), Postives = 53/119 (44.54%), Query Frame = 0

Query: 87  NRYEYVAVLFYASWCQFSKSFRPSF----SILSSLYPSIPHFAIQES-AVRPSILSKYGV 146
           N+++++ V FYA WC   K   P +    S LSS  P +    I  S        ++Y V
Sbjct: 45  NKHDFIVVEFYAPWCGHCKQLAPEYEKAASALSSNVPPVVLAKIDASEETNREFATQYEV 104

Query: 147 HGFPTLFLLNSTMRA--RYYGSRTLPSLAAFYNDVTGIQTASLDQISPDRIEQVWNQEK 199
            GFPT+ +  +  +A   Y G R    +  +    +G   AS +  S D   +V + +K
Sbjct: 105 QGFPTIKIFRNGGKAVQEYNGPREAEGIVTYLKKQSG--PASAEIKSADDASEVVSDKK 161

BLAST of Carg20538-RA vs. Swiss-Prot
Match: sp|Q9SA00|APRL4_ARATH (5'-adenylylsulfate reductase-like 4 OS=Arabidopsis thaliana OX=3702 GN=APRL4 PE=2 SV=1)

HSP 1 Score: 317.0 bits (811), Expect = 2.4e-85
Identity = 175/313 (55.91%), Postives = 230/313 (73.48%), Query Frame = 0

Query: 10  IVLLIVMLGRQASSADSVRVLHSGLSCPIESFLDTIFRFRDWNSYCPIDGQHG--HYEFI 69
           ++LL+V++    +  D+VRV      C  +S  D+IF  RD    C + G        F+
Sbjct: 6   LLLLLVIMFLTVADVDAVRVPF----CATKSAKDSIFGLRD--QTCSVSGVESDERPRFV 65

Query: 70  GVSEGDEASLQMALNMVHKNRYEYVAVLFYASWCQFSKSFRPSFSILSSLYPSIPHFAIQ 129
            V+EGDE  LQ+AL+M+HKN+ +YVA+LFYASWC FS+SFRPSF ++SSLY SIPHFAI+
Sbjct: 66  AVTEGDERWLQIALDMIHKNKCDYVALLFYASWCPFSRSFRPSFDVISSLYSSIPHFAIK 125

Query: 130 ESAVRPSILSKYGVHGFPTLFLLNSTMRARYYGSRTLPSLAAFYNDVTGIQTASLDQISP 189
           ES+++PS LSKYGVHGFPTL LLNSTMRARY G+R L SL AFY+DVTGI+T  LD+ S 
Sbjct: 126 ESSIKPSTLSKYGVHGFPTLLLLNSTMRARYRGTRMLDSLVAFYSDVTGIET--LDKTSL 185

Query: 190 DRIEQVWNQEKHDDSSEQENCPFSWARSPENLLREETYLALATAFVCMRLIYIFFPTLLV 249
           +R   V     +++++E ENCPF+WARSPEN+LR+ETYLALA  FV +RL+++ +PTL+V
Sbjct: 186 ERSVSV-PHLGNENNTEPENCPFTWARSPENMLRQETYLALAIVFVLLRLLHLIYPTLVV 245

Query: 250 YAQYVWQRHLRNLRLGTLWERPLTCLKGAVQLFSHFKDPCKRSNLQGGAMNARAWA-KSF 309
           + ++ W+R  +N+RL +L E  +  L  AVQL  H     +RSNLQGGAMNARAWA KS 
Sbjct: 246 FMKFTWRRIAQNMRLESLLEHTVGFLSRAVQLCMH-----RRSNLQGGAMNARAWASKSL 304

Query: 310 ATVSIGDASSSSR 320
           ATVSIGD+SSS+R
Sbjct: 306 ATVSIGDSSSSNR 304

BLAST of Carg20538-RA vs. Swiss-Prot
Match: sp|Q9ZPE9|APRL6_ARATH (5'-adenylylsulfate reductase-like 6 OS=Arabidopsis thaliana OX=3702 GN=APRL6 PE=2 SV=2)

HSP 1 Score: 256.1 bits (653), Expect = 5.0e-67
Identity = 156/313 (49.84%), Postives = 206/313 (65.81%), Query Frame = 0

Query: 10  IVLLIVMLGRQASSADSVRVLHSGLSCPIESFLDTIFRFRDWNSYCPIDGQHGHYEFIGV 69
           ++L++V+L    ++A +VRV      CP ES  D I  FRD ++          +    V
Sbjct: 8   LLLVVVVLFVNLTNA-TVRV----QICPRESAKDYILGFRDKSAL---------HRPGFV 67

Query: 70  SEGDEASLQMALNMV-HKNRYEYVAVLFYASWCQFSKSFRPSFSILSSLYPSIPHFAIQE 129
           +EGD+  LQMA +MV  KN+ +Y A+LFYASWC FS+  RPSF ++S LY S+PHFAI+E
Sbjct: 68  TEGDDRWLQMAADMVDKKNKCDYAALLFYASWCPFSRLVRPSFDLMSLLYSSVPHFAIEE 127

Query: 130 SAVRPSILSKYGVHGFPTLFLLNSTMRARYYGSRTLPSLAAFYNDVTGIQTASLDQISPD 189
           S+V+ S LSKYGVHGFPT+ L+NSTM   Y GSRTL SL AFY DVTGI+T     +  +
Sbjct: 128 SSVKASTLSKYGVHGFPTIILMNSTMLVVYRGSRTLDSLVAFYTDVTGIETMDERWVERN 187

Query: 190 RIEQVWNQEKHDDSSEQENCPFSWA-RSPENLLREETYLALATAFVCMRLIYIFFPTLLV 249
           R+   ++       +E ENCPF WA RSPENLLR+ETYL LAT FV +RL+++  PT++V
Sbjct: 188 RLVPHFH-------AEPENCPFPWARRSPENLLRQETYLTLATVFVLLRLLHLISPTMVV 247

Query: 250 YAQYVWQRHLRNLRLGTLWERPLTCLKGAVQLFSHFKDPCKRSNLQGGAMNARAWA-KSF 309
           + ++ W R + N+RLG   E  +T          + K+PC  SNLQ GAMNARAWA KS 
Sbjct: 248 FVKFTWGR-VSNMRLGNPLEHTVT---------MYLKEPCMSSNLQEGAMNARAWASKSL 289

Query: 310 ATVSIGDASSSSR 320
           ATVSI ++SSSSR
Sbjct: 308 ATVSIAESSSSSR 289

BLAST of Carg20538-RA vs. Swiss-Prot
Match: sp|Q84P95|APRL3_ORYSJ (5'-adenylylsulfate reductase-like 3 OS=Oryza sativa subsp. japonica OX=39947 GN=APRL3 PE=2 SV=1)

HSP 1 Score: 251.9 bits (642), Expect = 9.5e-66
Identity = 134/254 (52.76%), Postives = 179/254 (70.47%), Query Frame = 0

Query: 67  IGVSEGDEASLQMALNMVHKNRYEYVAVLFYASWCQFSKSFRPSFSILSSLYPSIPHFAI 126
           +GV EGDE +L  A+ ++H N+ +Y+AVLFYASWC FS+  +P+F IL+SL+PSI HFA 
Sbjct: 55  VGVIEGDEVTLAKAITLLHMNKDDYIAVLFYASWCPFSQECKPNFEILASLFPSIRHFAF 114

Query: 127 QESAVRPSILSKYGVHGFPTLFLLNSTMRARYYGSRTLPSLAAFYNDVTGIQTASLDQIS 186
           +ES++RPSI+S+YG+HGFPTLFLLNSTMR RY+G RT+ SLAAFY DV+G   +   +  
Sbjct: 115 EESSIRPSIISRYGIHGFPTLFLLNSTMRVRYHGPRTVKSLAAFYRDVSGFDVSMTSEAV 174

Query: 187 PDRIEQVWNQEKHDDSSEQENCPFSWARSPENLLREETYLALATAFVCMRLIYIFFPTLL 246
              ++ +  + K D  +EQENCPF WARSPE +L+++TYLALATAFV +RL+Y+ FP + 
Sbjct: 175 LHSVDGI--ELKKD--AEQENCPFWWARSPEKILQQDTYLALATAFVILRLLYLLFPKIG 234

Query: 247 VYAQYVWQRHLRNLRLGTLWERPLTCLKGAVQLFSHFKDPCKRSNLQGGAMNARAWA-KS 306
            +A+  W+RH     L  + E   T L+ A   F     P KR NLQ GA NA AWA KS
Sbjct: 235 SFAKRAWRRHTLFPNLVGVHEYFFTYLEQARHKFFRLY-PSKRGNLQEGARNATAWASKS 294

Query: 307 FATVSIGDASSSSR 320
            A+VSIG+ S+  R
Sbjct: 295 LASVSIGEPSTIGR 303

BLAST of Carg20538-RA vs. Swiss-Prot
Match: sp|Q67VZ8|APRL2_ORYSJ (5'-adenylylsulfate reductase-like 2 OS=Oryza sativa subsp. japonica OX=39947 GN=APRL2 PE=2 SV=1)

HSP 1 Score: 209.1 bits (531), Expect = 7.1e-53
Identity = 118/252 (46.83%), Postives = 163/252 (64.68%), Query Frame = 0

Query: 67  IGVSEGDEASLQMALNMVHKNRYEYVAVLFYASWCQFSKSFRPSFSILSSLYPSIPHFAI 126
           +GV+EGD+A L  A+N++H N+ ++ AVLFYASWC FS+  R  F  L+ ++P+I H AI
Sbjct: 50  LGVTEGDDAILARAVNLLHANKEDFAAVLFYASWCPFSQECRLRFEKLACIFPTIRHLAI 109

Query: 127 QESAVRPSILSKYGVHGFPTLFLLNSTMRARYYGSRTLPSLAAFYNDVTGIQTASLDQIS 186
           +ES VR     +YG+HG+PTLFL+NST+R RY+G RT+ SLAAFYNDV+GI  +    + 
Sbjct: 110 EESTVRLRTRYRYGIHGYPTLFLINSTVRVRYHGPRTVKSLAAFYNDVSGINPSMDPAVG 169

Query: 187 PDRIEQVWNQEKHDDSSEQENCPFSWARSPENLLREETYLALATAFVCMRLIYIFFPTLL 246
            D IE      K D   EQE C F  AR+PEN+L+ +TYL LA +FV +RL+Y+F+P + 
Sbjct: 170 DDNIE-----PKRD--CEQEKCLFWSARTPENILQPDTYLTLAASFVILRLLYLFYPKIT 229

Query: 247 VYAQYVWQRHLRNLRLGTLWERPLTCLKGAVQLFSHFKDPCKRSNLQGGAMNARAWA-KS 306
            + +  W R        TL+    TCL+     F+    P K+ NL  GA +A AWA KS
Sbjct: 230 AFVKRTWSRR-------TLF----TCLEQGKHKFNRVY-PSKQGNLHDGARHATAWASKS 282

Query: 307 FATVSIGDASSS 318
            A+VSIG+ S+S
Sbjct: 290 LASVSIGEPSTS 282

BLAST of Carg20538-RA vs. Swiss-Prot
Match: sp|Q5DJV7|APRL4_ORYSJ (5'-adenylylsulfate reductase-like 4 OS=Oryza sativa subsp. japonica OX=39947 GN=APRL4 PE=2 SV=1)

HSP 1 Score: 204.1 bits (518), Expect = 2.3e-51
Identity = 106/210 (50.48%), Postives = 143/210 (68.10%), Query Frame = 0

Query: 53  SYCPIDGQHGH-YEFIGVSEGDEASLQMALNMVHKNRYEYVAVLFYASWCQFSKSFRPSF 112
           S CP  G  GH    +GV EGD+  LQ A+ +V +NR ++VA+LFYASWC FSK FR  F
Sbjct: 48  SSCPAAGSPGHRAHHVGVVEGDDFVLQKAVTLVLQNREDFVAILFYASWCPFSKIFRTDF 107

Query: 113 SILSSLYPSIPHFAIQESAVRPSILSKYGVHGFPTLFLLNSTMRARYYGSRTLPSLAAFY 172
             LSS +P+I HF+ +ES ++P +LS+YGV  FPTLFL+NSTMR RY+GSRT+ SLA FY
Sbjct: 108 QKLSSFFPTIAHFSFEESRIKPRMLSRYGVRAFPTLFLVNSTMRVRYHGSRTMNSLAMFY 167

Query: 173 NDVTGIQTASLDQISPDRIEQVWNQEKHDDSSEQENCPFSWARSPENLLREETYLALATA 232
            DVTG+   SLD IS +R+E+V N  ++D  +EQ +  F +ARSP+ LL ++T LALA++
Sbjct: 168 KDVTGMNPVSLDAISLERMEEVVNIIENDKKTEQGDSLFMFARSPDRLLHQDTCLALASS 227

Query: 233 FVCMRLIYIFFPTLLVYAQYVWQRHLRNLR 262
           FV MRL+    P L    +  W+     L+
Sbjct: 228 FVLMRLLCFLLPKLNACVKQAWRMQFYELK 257

BLAST of Carg20538-RA vs. TrEMBL
Match: tr|A0A0A0LRQ5|A0A0A0LRQ5_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G368970 PE=4 SV=1)

HSP 1 Score: 542.7 bits (1397), Expect = 5.5e-151
Identity = 275/319 (86.21%), Postives = 287/319 (89.97%), Query Frame = 0

Query: 4   GFCVRGIVLLIVMLGRQASSADSVRVLHSGLSCPIESFLDTIFRFRDWNSYCPIDGQHGH 63
           GF V GI+L  ++LG       SVRV  S   CP ESFLDTIFRFRD NS CP  G   H
Sbjct: 7   GFWVIGILLFSLLLG----FTHSVRVSDSPPFCPAESFLDTIFRFRDSNSNCPFHGDVRH 66

Query: 64  YEFIGVSEGDEASLQMALNMVHKNRYEYVAVLFYASWCQFSKSFRPSFSILSSLYPSIPH 123
           YEFIGVSEGDEASLQMALNMVH NRYEYV+VLFYASWC FSKSFRPSFSILSSLY SIPH
Sbjct: 67  YEFIGVSEGDEASLQMALNMVHSNRYEYVSVLFYASWCPFSKSFRPSFSILSSLYASIPH 126

Query: 124 FAIQESAVRPSILSKYGVHGFPTLFLLNSTMRARYYGSRTLPSLAAFYNDVTGIQTASLD 183
           FAIQESAVRPSILSKYGVHGFPTLFLLNSTMRARYYGSRTL SL AFYNDVTGIQTASLD
Sbjct: 127 FAIQESAVRPSILSKYGVHGFPTLFLLNSTMRARYYGSRTLSSLVAFYNDVTGIQTASLD 186

Query: 184 QISPDRIEQVWNQEKHDDSSEQENCPFSWARSPENLLREETYLALATAFVCMRLIYIFFP 243
           QIS DRI QVWN+EKHDD+SEQENCPFSWARSPENLLREETYLALATAFV MRLIYIFFP
Sbjct: 187 QISSDRIGQVWNREKHDDNSEQENCPFSWARSPENLLREETYLALATAFVLMRLIYIFFP 246

Query: 244 TLLVYAQYVWQRHLRNLRLGTLWERPLTCLKGAVQLFSHFKDPCKRSNLQGGAMNARAWA 303
           TLLVYA+YVW+RHLRN+RLGTLWERPLTC+KGA+QLFSHFKDPCKRSNLQGGAMNA+AWA
Sbjct: 247 TLLVYARYVWRRHLRNMRLGTLWERPLTCMKGAIQLFSHFKDPCKRSNLQGGAMNAKAWA 306

Query: 304 KSFATVSIGDASSSSRVCQ 323
           KSFATVSIGDASSSSRVCQ
Sbjct: 307 KSFATVSIGDASSSSRVCQ 321

BLAST of Carg20538-RA vs. TrEMBL
Match: tr|A0A1S3BC24|A0A1S3BC24_CUCME (5'-adenylylsulfate reductase-like 4 OS=Cucumis melo OX=3656 GN=LOC103488066 PE=4 SV=1)

HSP 1 Score: 542.0 bits (1395), Expect = 9.3e-151
Identity = 274/319 (85.89%), Postives = 287/319 (89.97%), Query Frame = 0

Query: 4   GFCVRGIVLLIVMLGRQASSADSVRVLHSGLSCPIESFLDTIFRFRDWNSYCPIDGQHGH 63
           GF V GI+L  ++LG     A SVR   S   CP ESFLDTIFRFRD  S CP  G   H
Sbjct: 7   GFWVIGILLFSLLLG----LAHSVRAPDSPPFCPAESFLDTIFRFRDSYSNCPFHGDVRH 66

Query: 64  YEFIGVSEGDEASLQMALNMVHKNRYEYVAVLFYASWCQFSKSFRPSFSILSSLYPSIPH 123
           YEFIGVSEGDEASLQMALNMVH NRYEYV+VLFYASWC FSKSFRPSFSILSSLY SIPH
Sbjct: 67  YEFIGVSEGDEASLQMALNMVHSNRYEYVSVLFYASWCPFSKSFRPSFSILSSLYASIPH 126

Query: 124 FAIQESAVRPSILSKYGVHGFPTLFLLNSTMRARYYGSRTLPSLAAFYNDVTGIQTASLD 183
           FAIQESAVRPSILSKYGVHGFPTLFLLNSTMRARYYGSRTL SL AFYNDVTGIQTASLD
Sbjct: 127 FAIQESAVRPSILSKYGVHGFPTLFLLNSTMRARYYGSRTLSSLVAFYNDVTGIQTASLD 186

Query: 184 QISPDRIEQVWNQEKHDDSSEQENCPFSWARSPENLLREETYLALATAFVCMRLIYIFFP 243
           QISPDRI QVWN+EKHDD+SEQENCPFSWARSPENLLREETYLALATAFV MRL+YIFFP
Sbjct: 187 QISPDRIGQVWNREKHDDNSEQENCPFSWARSPENLLREETYLALATAFVLMRLVYIFFP 246

Query: 244 TLLVYAQYVWQRHLRNLRLGTLWERPLTCLKGAVQLFSHFKDPCKRSNLQGGAMNARAWA 303
           TLLVYA+YVW+RHLRN+RLGTLWERPLTC+KGA+QLFSHFKDPCKRSNLQGGAMNA+AWA
Sbjct: 247 TLLVYARYVWRRHLRNMRLGTLWERPLTCMKGAIQLFSHFKDPCKRSNLQGGAMNAKAWA 306

Query: 304 KSFATVSIGDASSSSRVCQ 323
           KSFATVSIGDASSSSRVCQ
Sbjct: 307 KSFATVSIGDASSSSRVCQ 321

BLAST of Carg20538-RA vs. TrEMBL
Match: tr|A0A067GMW0|A0A067GMW0_CITSI (Uncharacterized protein OS=Citrus sinensis OX=2711 GN=CISIN_1g020846mg PE=4 SV=1)

HSP 1 Score: 404.1 bits (1037), Expect = 3.0e-109
Identity = 208/312 (66.67%), Postives = 253/312 (81.09%), Query Frame = 0

Query: 11  VLLIVMLGRQASSADSVRVLHSGLSCPIESFLDTIFRFRDWNSYCPIDGQHGHYEFIGVS 70
           +L++V+ GR   +  +VR       CP ES  D+IFRF+D  S+CPI   +   + +GV 
Sbjct: 10  ILMLVLWGRLTCAVVAVRF----PLCPTESVTDSIFRFQD--SFCPI-SCNDFQDIVGVI 69

Query: 71  EGDEASLQMALNMVHKNRYEYVAVLFYASWCQFSKSFRPSFSILSSLYPSIPHFAIQESA 130
           EGDE SLQMALNMVHKN +EYVAVLFYASWC FS++FRPSFS+LSSLY SIPHFAI+ESA
Sbjct: 70  EGDEVSLQMALNMVHKNSHEYVAVLFYASWCPFSRNFRPSFSVLSSLYSSIPHFAIEESA 129

Query: 131 VRPSILSKYGVHGFPTLFLLNSTMRARYYGSRTLPSLAAFYNDVTGIQTASLDQISPDRI 190
           +RPSILSKYGVHGFPTLFLLNS+MR RY+GSRTL SL AFY+DVTG+ TASLD+ISPD++
Sbjct: 130 IRPSILSKYGVHGFPTLFLLNSSMRVRYHGSRTLDSLVAFYSDVTGMNTASLDKISPDKV 189

Query: 191 EQVWNQEKHDDSSEQENCPFSWARSPENLLREETYLALATAFVCMRLIYIFFPTLLVYAQ 250
            +  N EKH +++E+E+CPFSWARSPENLL++ETYLALATAFV +RL+YIF PTLL++AQ
Sbjct: 190 GKASNHEKH-NNTEEESCPFSWARSPENLLQQETYLALATAFVLLRLVYIFLPTLLIFAQ 249

Query: 251 YVWQRHLRNLRLGTLWERPLTCLKGAVQLFSHFKDPCKRSNLQGGAMNARAWA-KSFATV 310
           + W+R +RNL+LG+L E P T L  A+QLF    +PCKRSNLQ GA+NARAWA KS ATV
Sbjct: 250 FTWRRLIRNLKLGSLLEHPRTYLNRAIQLFKTLNEPCKRSNLQEGALNARAWASKSLATV 309

Query: 311 SIGDASSSSRVC 322
           SIGDASSS   C
Sbjct: 310 SIGDASSSRGAC 313

BLAST of Carg20538-RA vs. TrEMBL
Match: tr|V4T274|V4T274_9ROSI (Uncharacterized protein OS=Citrus clementina OX=85681 GN=CICLE_v10001862mg PE=4 SV=1)

HSP 1 Score: 398.7 bits (1023), Expect = 1.3e-107
Identity = 207/312 (66.35%), Postives = 251/312 (80.45%), Query Frame = 0

Query: 11  VLLIVMLGRQASSADSVRVLHSGLSCPIESFLDTIFRFRDWNSYCPIDGQHGHYEFIGVS 70
           +L++V+ GR   +  +VR       CP ES  D+IFRF+D  S+CPI   +   + +GV 
Sbjct: 10  ILMLVLWGRLTCAVVAVRF----PLCPTESVTDSIFRFQD--SFCPI-SCNDFQDIVGVI 69

Query: 71  EGDEASLQMALNMVHKNRYEYVAVLFYASWCQFSKSFRPSFSILSSLYPSIPHFAIQESA 130
           EGDE SLQMALNMV KN +EYVAVLFYASWC FS++FRPSFS+LSSLY SIPHFAI+ESA
Sbjct: 70  EGDEVSLQMALNMVQKNSHEYVAVLFYASWCPFSRNFRPSFSVLSSLYSSIPHFAIEESA 129

Query: 131 VRPSILSKYGVHGFPTLFLLNSTMRARYYGSRTLPSLAAFYNDVTGIQTASLDQISPDRI 190
           +RPSILSKYGVHGFPTLFLLNS+MR RY+GSRTL SL AFY+DVTG+ TASLD+ISPD++
Sbjct: 130 IRPSILSKYGVHGFPTLFLLNSSMRVRYHGSRTLDSLVAFYSDVTGMNTASLDKISPDKV 189

Query: 191 EQVWNQEKHDDSSEQENCPFSWARSPENLLREETYLALATAFVCMRLIYIFFPTLLVYAQ 250
            +  N EKH +++E+E+CPFSWARSPENLL++ETYLALATAFV +RL+YIF PTLLV+AQ
Sbjct: 190 GKASNHEKH-NNTEEESCPFSWARSPENLLQQETYLALATAFVLLRLVYIFLPTLLVFAQ 249

Query: 251 YVWQRHLRNLRLGTLWERPLTCLKGAVQLFSHFKDPCKRSNLQGGAMNARAWA-KSFATV 310
           + W+R +RNL+ G+L E P T L  A+QLF    +PCKRSNLQ GA+NARAWA KS ATV
Sbjct: 250 FTWRRLIRNLKPGSLLEHPRTYLNRAIQLFKTLNEPCKRSNLQEGALNARAWASKSLATV 309

Query: 311 SIGDASSSSRVC 322
           SIGDASSS   C
Sbjct: 310 SIGDASSSRGAC 313

BLAST of Carg20538-RA vs. TrEMBL
Match: tr|A0A2I4DZ49|A0A2I4DZ49_9ROSI (5'-adenylylsulfate reductase-like 4 OS=Juglans regia OX=51240 GN=LOC108984813 PE=4 SV=1)

HSP 1 Score: 397.5 bits (1020), Expect = 2.8e-107
Identity = 206/310 (66.45%), Postives = 242/310 (78.06%), Query Frame = 0

Query: 9   GIVLLIVMLGRQASSADSVRVLHSGLSCPIESFLDTIFRFRDWNSYCPIDGQHGHYEFIG 68
           GIV+ +++  R   +A+  R   S   CP+ES  D+I  FR WN  CP+ G  G    + 
Sbjct: 7   GIVVALLLWVRFTCAAEPSRASSSSTLCPVESASDSILGFRLWN--CPLHGDSGSPGLVA 66

Query: 69  VSEGDEASLQMALNMVHKNRYEYVAVLFYASWCQFSKSFRPSFSILSSLYPSIPHFAIQE 128
           V+ GDE SLQ ALNMVH   ++YVAVLFYASWC FS++F PSFSILSSLYPSIPHFAI+E
Sbjct: 67  VTVGDEVSLQRALNMVHTKGHQYVAVLFYASWCPFSRTFWPSFSILSSLYPSIPHFAIEE 126

Query: 129 SAVRPSILSKYGVHGFPTLFLLNSTMRARYYGSRTLPSLAAFYNDVTGIQTASLDQISPD 188
           S VRPSILSKYGVHGFPTLFLLNSTMR RY GSRTL SLAAFY DVTGI+TASLDQIS +
Sbjct: 127 STVRPSILSKYGVHGFPTLFLLNSTMRVRYLGSRTLSSLAAFYGDVTGIKTASLDQISIE 186

Query: 189 RIEQVWNQEKHDDSSEQENCPFSWARSPENLLREETYLALATAFVCMRLIYIFFPTLLVY 248
            I  + N EKH D++EQE+CPFSWARSPE LLR+ETYLALAT FV +RL+Y  FPT+L+ 
Sbjct: 187 NIGCLSNHEKH-DNTEQESCPFSWARSPEKLLRQETYLALATTFVLLRLLYFSFPTVLLC 246

Query: 249 AQYVWQRHLRNLRLGTLWERPLTCLKGAVQLFSHFKDPCKRSNLQGGAMNARAWA-KSFA 308
           A+Y W+RH+RN+RLG LWE PL  L+ A+QLF+   +PCKRSNLQ GAMNARAWA KS A
Sbjct: 247 ARYAWRRHIRNVRLGGLWEHPLAYLRRAIQLFNSLNEPCKRSNLQEGAMNARAWASKSLA 306

Query: 309 TVSIGDASSS 318
           TVSIGDA +S
Sbjct: 307 TVSIGDAGNS 313

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022952011.12.4e-18299.695'-adenylylsulfate reductase-like 4 [Cucurbita moschata] >XP_023538087.1 5'-aden... [more]
XP_023002667.17.6e-18198.765'-adenylylsulfate reductase-like 4 [Cucurbita maxima][more]
XP_022131641.11.3e-15988.515'-adenylylsulfate reductase-like 4 [Momordica charantia][more]
XP_022997216.14.0e-15386.085'-adenylylsulfate reductase-like 4 [Cucurbita maxima][more]
XP_023545571.13.7e-15184.495'-adenylylsulfate reductase-like 4 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT1G34780.11.3e-8655.91APR-like 4[more]
AT4G08930.12.8e-6849.84APR-like 6[more]
AT3G03860.15.5e-3233.33APR-like 5[more]
AT5G18120.15.0e-2531.18APR-like 7[more]
AT1G21750.18.3e-0427.73PDI-like 1-1[more]
Match NameE-valueIdentityDescription
sp|Q9SA00|APRL4_ARATH2.4e-8555.915'-adenylylsulfate reductase-like 4 OS=Arabidopsis thaliana OX=3702 GN=APRL4 PE=... [more]
sp|Q9ZPE9|APRL6_ARATH5.0e-6749.845'-adenylylsulfate reductase-like 6 OS=Arabidopsis thaliana OX=3702 GN=APRL6 PE=... [more]
sp|Q84P95|APRL3_ORYSJ9.5e-6652.765'-adenylylsulfate reductase-like 3 OS=Oryza sativa subsp. japonica OX=39947 GN=... [more]
sp|Q67VZ8|APRL2_ORYSJ7.1e-5346.835'-adenylylsulfate reductase-like 2 OS=Oryza sativa subsp. japonica OX=39947 GN=... [more]
sp|Q5DJV7|APRL4_ORYSJ2.3e-5150.485'-adenylylsulfate reductase-like 4 OS=Oryza sativa subsp. japonica OX=39947 GN=... [more]
Match NameE-valueIdentityDescription
tr|A0A0A0LRQ5|A0A0A0LRQ5_CUCSA5.5e-15186.21Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G368970 PE=4 SV=1[more]
tr|A0A1S3BC24|A0A1S3BC24_CUCME9.3e-15185.895'-adenylylsulfate reductase-like 4 OS=Cucumis melo OX=3656 GN=LOC103488066 PE=4... [more]
tr|A0A067GMW0|A0A067GMW0_CITSI3.0e-10966.67Uncharacterized protein OS=Citrus sinensis OX=2711 GN=CISIN_1g020846mg PE=4 SV=1[more]
tr|V4T274|V4T274_9ROSI1.3e-10766.35Uncharacterized protein OS=Citrus clementina OX=85681 GN=CICLE_v10001862mg PE=4 ... [more]
tr|A0A2I4DZ49|A0A2I4DZ49_9ROSI2.8e-10766.455'-adenylylsulfate reductase-like 4 OS=Juglans regia OX=51240 GN=LOC108984813 PE... [more]
The following terms have been associated with this mRNA:
Vocabulary: Biological Process
TermDefinition
GO:0045454cell redox homeostasis
Vocabulary: INTERPRO
TermDefinition
IPR036249Thioredoxin-like_sf
IPR013766Thioredoxin_domain
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0045454 cell redox homeostasis
cellular_component GO:0005575 cellular_component
molecular_function GO:0003674 molecular_function

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Carg20538Carg20538gene


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Carg20538-RACarg20538-RA-proteinpolypeptide


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg20538-RA.exon.1Carg20538-RA.exon.1exon
Carg20538-RA.exon.2Carg20538-RA.exon.2exon
Carg20538-RA.exon.3Carg20538-RA.exon.3exon
Carg20538-RA.exon.4Carg20538-RA.exon.4exon


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg20538-RA.five_prime_UTR.1Carg20538-RA.five_prime_UTR.1five_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg20538-RA.CDS.1Carg20538-RA.CDS.1CDS
Carg20538-RA.CDS.2Carg20538-RA.CDS.2CDS
Carg20538-RA.CDS.3Carg20538-RA.CDS.3CDS
Carg20538-RA.CDS.4Carg20538-RA.CDS.4CDS


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3DG3DSA:3.40.30.10coord: 71..184
e-value: 1.6E-13
score: 52.4
NoneNo IPR availablePANTHERPTHR18929:SF1695'-ADENYLYLSULFATE REDUCTASE-LIKE 4-RELATEDcoord: 8..320
NoneNo IPR availablePANTHERPTHR18929PROTEIN DISULFIDE ISOMERASEcoord: 8..320
NoneNo IPR availableCDDcd02999PDI_a_ERp44_likecoord: 72..171
e-value: 7.39317E-48
score: 157.135
IPR013766Thioredoxin domainPFAMPF00085Thioredoxincoord: 88..170
e-value: 4.1E-8
score: 33.0
IPR013766Thioredoxin domainPROSITEPS51352THIOREDOXIN_2coord: 69..176
score: 8.802
IPR036249Thioredoxin-like superfamilySUPERFAMILYSSF52833Thioredoxin-likecoord: 88..176