Carg18075-RA (mRNA) Silver-seed gourd

NameCarg18075-RA
TypemRNA
OrganismCucurbita argyrosperma (Silver-seed gourd)
Descriptioncalcium/calmodulin-dependent protein kinase type IV-like
LocationCucurbita_argyrosperma_scaffold_095 : 262000 .. 262470 (+)
Sequence length471
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGCTTTTGATCTCAGCTTCTCTGCTCTCTGCAATGGTGTTTTACTCTTCTCTTCTCCCTTTCCTCCACTCTTTCAACCTTTATATCTCTGCAACAGCTCACAAGAACTACATGTTCTTGCTCTGCAATGGCCTCCTTGTCTTCATTGTTCGAAACTCCGGTCTTATCAGCTGCTCCGGTGACCGACACAGCAGCGGAGATAGCAGGGGAGCTGAAGCTCTAGAGGCTTCCATGGCGGATGGCAGCAGCAAAATTGAGAGCAAAGTTGAAGATAGAGAAGAAAGGGGAAATGGGTTGATTCAAAAAGATGAAGAAAGTGAAAGATCGATCATGGAGGATGACGATGACGACGACGAAGAAGAAGTTGGGGCATTGAGCAAAGAGGAATTGAATAAGAAATGTGAAGAATTCATTAGAAAAATGAAGGCTGGAATCAAGCTTGAATCACAGCAATTGCTTGTTTTGTAA

mRNA sequence

ATGAAGCTTTTGATCTCAGCTTCTCTGCTCTCTGCAATGGTGTTTTACTCTTCTCTTCTCCCTTTCCTCCACTCTTTCAACCTTTATATCTCTGCAACAGCTCACAAGAACTACATGTTCTTGCTCTGCAATGGCCTCCTTGTCTTCATTGTTCGAAACTCCGGTCTTATCAGCTGCTCCGGTGACCGACACAGCAGCGGAGATAGCAGGGGAGCTGAAGCTCTAGAGGCTTCCATGGCGGATGGCAGCAGCAAAATTGAGAGCAAAGTTGAAGATAGAGAAGAAAGGGGAAATGGGTTGATTCAAAAAGATGAAGAAAGTGAAAGATCGATCATGGAGGATGACGATGACGACGACGAAGAAGAAGTTGGGGCATTGAGCAAAGAGGAATTGAATAAGAAATGTGAAGAATTCATTAGAAAAATGAAGGCTGGAATCAAGCTTGAATCACAGCAATTGCTTGTTTTGTAA

Coding sequence (CDS)

ATGAAGCTTTTGATCTCAGCTTCTCTGCTCTCTGCAATGGTGTTTTACTCTTCTCTTCTCCCTTTCCTCCACTCTTTCAACCTTTATATCTCTGCAACAGCTCACAAGAACTACATGTTCTTGCTCTGCAATGGCCTCCTTGTCTTCATTGTTCGAAACTCCGGTCTTATCAGCTGCTCCGGTGACCGACACAGCAGCGGAGATAGCAGGGGAGCTGAAGCTCTAGAGGCTTCCATGGCGGATGGCAGCAGCAAAATTGAGAGCAAAGTTGAAGATAGAGAAGAAAGGGGAAATGGGTTGATTCAAAAAGATGAAGAAAGTGAAAGATCGATCATGGAGGATGACGATGACGACGACGAAGAAGAAGTTGGGGCATTGAGCAAAGAGGAATTGAATAAGAAATGTGAAGAATTCATTAGAAAAATGAAGGCTGGAATCAAGCTTGAATCACAGCAATTGCTTGTTTTGTAA

Protein sequence

MKLLISASLLSAMVFYSSLLPFLHSFNLYISATAHKNYMFLLCNGLLVFIVRNSGLISCSGDRHSSGDSRGAEALEASMADGSSKIESKVEDREERGNGLIQKDEESERSIMEDDDDDDEEEVGALSKEELNKKCEEFIRKMKAGIKLESQQLLVL
BLAST of Carg18075-RA vs. NCBI nr
Match: XP_023530308.1 (uncharacterized protein LOC111792915 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 236.5 bits (602), Expect = 6.1e-59
Identity = 154/156 (98.72%), Postives = 155/156 (99.36%), Query Frame = 0

Query: 1   MKLLISASLLSAMVFYSSLLPFLHSFNLYISATAHKNYMFLLCNGLLVFIVRNSGLISCS 60
           MKLLISASLLSAMVFYSSLLPFLHSFNLYISATAHKNYMFLLCNGLLVFIVRNSGLISCS
Sbjct: 1   MKLLISASLLSAMVFYSSLLPFLHSFNLYISATAHKNYMFLLCNGLLVFIVRNSGLISCS 60

Query: 61  GDRHSSGDSRGAEALEASMADGSSKIESKVEDREERGNGLIQKXXXXXXXXXXXXXXXXX 120
           GDRHS GDSRGAEALEA+MADGSSKIESKVEDREERGNGLIQKXXXXXXXXXXXXXXXXX
Sbjct: 61  GDRHSGGDSRGAEALEAAMADGSSKIESKVEDREERGNGLIQKXXXXXXXXXXXXXXXXX 120

Query: 121 XXXXALSKEELNKKCEEFIRKMKAGIKLESQQLLVL 157
           XXXXALSKEELNKKCEEFIRKMKAGIKLESQQLLVL
Sbjct: 121 XXXXALSKEELNKKCEEFIRKMKAGIKLESQQLLVL 156

BLAST of Carg18075-RA vs. NCBI nr
Match: XP_022928452.1 (uncharacterized protein LOC111435260 [Cucurbita moschata])

HSP 1 Score: 232.3 bits (591), Expect = 1.2e-57
Identity = 155/159 (97.48%), Postives = 155/159 (97.48%), Query Frame = 0

Query: 1   MKLLISASLLSAMVFYSSLLPFLHSFNLYISATAHKNYMFLLCNGLLVFIVRNSGLISCS 60
           MKLLISASLLSAMVFYSSLLPFLHSFNLYISATAHKNYMFLLCNGLLVFIVRNSGLISCS
Sbjct: 1   MKLLISASLLSAMVFYSSLLPFLHSFNLYISATAHKNYMFLLCNGLLVFIVRNSGLISCS 60

Query: 61  GDRHSSGDSRGAEALEASMADGSSKIESKVEDREERGNGLIQK---XXXXXXXXXXXXXX 120
           GDRHS GDSRGAEALEASMADGSSKIESKVEDREERGNGLIQK   XXXXXXXXXXXXXX
Sbjct: 61  GDRHSGGDSRGAEALEASMADGSSKIESKVEDREERGNGLIQKXXXXXXXXXXXXXXXXX 120

Query: 121 XXXXXXXALSKEELNKKCEEFIRKMKAGIKLESQQLLVL 157
           XXXXXXXALSKEELNKKCEEFIRKMKAGIKLESQQLLVL
Sbjct: 121 XXXXXXXALSKEELNKKCEEFIRKMKAGIKLESQQLLVL 159

BLAST of Carg18075-RA vs. NCBI nr
Match: XP_011658666.1 (PREDICTED: uncharacterized protein LOC105436067 [Cucumis sativus] >KGN43414.1 hypothetical protein Csa_7G032240 [Cucumis sativus])

HSP 1 Score: 160.2 bits (404), Expect = 5.6e-36
Identity = 114/162 (70.37%), Postives = 123/162 (75.93%), Query Frame = 0

Query: 1   MKLLISASLLSAMVFYSSLLPFLHSFNLYISATAHKNYMFLLCNGLLVFIVRNSGLISCS 60
           MKL+ISASLLSAMVFYSSLLPFLHS NLYIS+T HKNYMFLLCNGLLVFIVRNSGLI  +
Sbjct: 1   MKLVISASLLSAMVFYSSLLPFLHSLNLYISSTIHKNYMFLLCNGLLVFIVRNSGLIGNT 60

Query: 61  GDRHSSGDSRGAEAL-EASMADGSSKIESKVEDREERGNGLIQ-----KXXXXXXXXXXX 120
            D  SSG+    EAL  A+MADG+ KIESKVE  EERGNGL+      +         XX
Sbjct: 61  SDERSSGN----EALGAATMADGNRKIESKVEIEEERGNGLVVNEDKIENLITDNEVEXX 120

Query: 121 XXXXXXXXXXALSKEELNKKCEEFIRKMKAGIKLESQQLLVL 157
           XXXXXXXXXX      LNKKCEEFIRKMKAGIK ESQQL +L
Sbjct: 121 XXXXXXXXXXXXXXXXLNKKCEEFIRKMKAGIKFESQQLFIL 158

BLAST of Carg18075-RA vs. NCBI nr
Match: XP_022969273.1 (sodium/potassium/calcium exchanger 1-like [Cucurbita maxima])

HSP 1 Score: 132.9 bits (333), Expect = 9.5e-28
Identity = 112/169 (66.27%), Postives = 118/169 (69.82%), Query Frame = 0

Query: 1   MKLLISASLLSAMVFYSSLLPFLHSFNLYISATAHKNYMFLLCNGLLVFIVRNSGLISCS 60
           MKL+ISAS+LSAMVFYSSLLPFLHS NLYISAT HKNYMFLLCNGLLVFIVRNSGLIS S
Sbjct: 1   MKLVISASVLSAMVFYSSLLPFLHSLNLYISATIHKNYMFLLCNGLLVFIVRNSGLISSS 60

Query: 61  GDRHSSG-DSRGAEALEASMADG------------SSKIESKVEDREERGNGLIQKXXXX 120
           G     G  SR AEAL A+M D                                  XXXX
Sbjct: 61  GREQCGGHGSREAEALGAAMEDSXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 120

Query: 121 XXXXXXXXXXXXXXXXXALSKEELNKKCEEFIRKMKAGIKLESQQLLVL 157
           XXXXXXXXXXXXXXX  ALS EEL+KK EEFIRKMKAGIKLESQQ+L+L
Sbjct: 121 XXXXXXXXXXXXXXXVGALSNEELDKKFEEFIRKMKAGIKLESQQVLIL 169

BLAST of Carg18075-RA vs. NCBI nr
Match: XP_008462077.2 (PREDICTED: gem-associated protein 2-like [Cucumis melo])

HSP 1 Score: 114.4 bits (285), Expect = 3.5e-22
Identity = 103/163 (63.19%), Postives = 110/163 (67.48%), Query Frame = 0

Query: 13  MVFYSSLLPFLHSFNLYISATAHKNYMFLLCNGLLVFIVRNSGLISCSGDRHSSGDSRGA 72
           MVFYSSLLPFLHS NLYIS+T HKNYMFLLCNGLLVFIVRNSGLI  + +  SS +    
Sbjct: 1   MVFYSSLLPFLHSLNLYISSTIHKNYMFLLCNGLLVFIVRNSGLIGNTCNEQSSRN---- 60

Query: 73  EALEA-SMADGSSKIESKVEDREE------------------RGNGLIQKXXXXXXXXXX 132
           EAL A +MADG+ KIESKVE   E                          XXXXXXXXXX
Sbjct: 61  EALGAVTMADGNRKIESKVEVEVEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 120

Query: 133 XXXXXXXXXXXALSKEELNKKCEEFIRKMKAGIKLESQQLLVL 157
           XXXXXXXXXXX       NKKCEEFIRKMKAGIK ESQQL +L
Sbjct: 121 XXXXXXXXXXXXXXXXXXNKKCEEFIRKMKAGIKFESQQLFIL 159

BLAST of Carg18075-RA vs. TrEMBL
Match: tr|A0A0A0K349|A0A0A0K349_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G032240 PE=4 SV=1)

HSP 1 Score: 160.2 bits (404), Expect = 3.7e-36
Identity = 114/162 (70.37%), Postives = 123/162 (75.93%), Query Frame = 0

Query: 1   MKLLISASLLSAMVFYSSLLPFLHSFNLYISATAHKNYMFLLCNGLLVFIVRNSGLISCS 60
           MKL+ISASLLSAMVFYSSLLPFLHS NLYIS+T HKNYMFLLCNGLLVFIVRNSGLI  +
Sbjct: 1   MKLVISASLLSAMVFYSSLLPFLHSLNLYISSTIHKNYMFLLCNGLLVFIVRNSGLIGNT 60

Query: 61  GDRHSSGDSRGAEAL-EASMADGSSKIESKVEDREERGNGLIQ-----KXXXXXXXXXXX 120
            D  SSG+    EAL  A+MADG+ KIESKVE  EERGNGL+      +         XX
Sbjct: 61  SDERSSGN----EALGAATMADGNRKIESKVEIEEERGNGLVVNEDKIENLITDNEVEXX 120

Query: 121 XXXXXXXXXXALSKEELNKKCEEFIRKMKAGIKLESQQLLVL 157
           XXXXXXXXXX      LNKKCEEFIRKMKAGIK ESQQL +L
Sbjct: 121 XXXXXXXXXXXXXXXXLNKKCEEFIRKMKAGIKFESQQLFIL 158

BLAST of Carg18075-RA vs. TrEMBL
Match: tr|A0A1S3CG16|A0A1S3CG16_CUCME (gem-associated protein 2-like OS=Cucumis melo OX=3656 GN=LOC103500515 PE=4 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 2.3e-22
Identity = 103/163 (63.19%), Postives = 110/163 (67.48%), Query Frame = 0

Query: 13  MVFYSSLLPFLHSFNLYISATAHKNYMFLLCNGLLVFIVRNSGLISCSGDRHSSGDSRGA 72
           MVFYSSLLPFLHS NLYIS+T HKNYMFLLCNGLLVFIVRNSGLI  + +  SS +    
Sbjct: 1   MVFYSSLLPFLHSLNLYISSTIHKNYMFLLCNGLLVFIVRNSGLIGNTCNEQSSRN---- 60

Query: 73  EALEA-SMADGSSKIESKVEDREE------------------RGNGLIQKXXXXXXXXXX 132
           EAL A +MADG+ KIESKVE   E                          XXXXXXXXXX
Sbjct: 61  EALGAVTMADGNRKIESKVEVEVEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 120

Query: 133 XXXXXXXXXXXALSKEELNKKCEEFIRKMKAGIKLESQQLLVL 157
           XXXXXXXXXXX       NKKCEEFIRKMKAGIK ESQQL +L
Sbjct: 121 XXXXXXXXXXXXXXXXXXNKKCEEFIRKMKAGIKFESQQLFIL 159

BLAST of Carg18075-RA vs. TrEMBL
Match: tr|A0A2C9UUD3|A0A2C9UUD3_MANES (Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_12G066000 PE=4 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 1.6e-10
Identity = 53/148 (35.81%), Postives = 81/148 (54.73%), Query Frame = 0

Query: 9   LLSAMVFYSSLLPFLHSFNLYISATAHKNYMFLLCNGLLVFIVRNSGLISCSGDRHSSGD 68
           L + ++ YSSLLPF+ +   + S+   KNYMFLLCNG+LVFIV+NSGL+  S        
Sbjct: 28  LFAVLLLYSSLLPFIFNLMQFFSSNIGKNYMFLLCNGILVFIVKNSGLVGSS--HQGINL 87

Query: 69  SRGAEALEASMADGSSKIESKVEDREERGNGLIQKXXXXXXXXXXXXXXXXXXXXXALSK 128
            +  E L+  +   S+  E +V +  ++   +++                       L+ 
Sbjct: 88  IKNGENLQKQLETVSA--EEQVHEIADKSLVMVE----------GVDEDDDDEEIGLLNV 147

Query: 129 EELNKKCEEFIRKMKAGIKLESQQLLVL 157
           EELNKKC++FIR MK  IK E+QQL+++
Sbjct: 148 EELNKKCDDFIRNMKERIKFEAQQLVLV 161

BLAST of Carg18075-RA vs. TrEMBL
Match: tr|A0A2C9UVE4|A0A2C9UVE4_MANES (Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_12G064700 PE=4 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 3.5e-10
Identity = 83/163 (50.92%), Postives = 105/163 (64.42%), Query Frame = 0

Query: 2   KLLISASLLSAMVFYSSLLPFLHSFNL--YISATAHKNYMFLLCNGLLVFIVRNSGLISC 61
           KL  S  L S ++  SSL PF+ +F L  ++S    KNYMFLLCNG+LV IV+NSGLI+ 
Sbjct: 27  KLPASVFLFSVILLCSSLFPFI-TFKLMQFLSCKISKNYMFLLCNGILVLIVKNSGLINK 86

Query: 62  SGDRHS---SGDS---RGAEALEASMADGSSKIESKVEDREERGNGLIQKXXXXXXXXXX 121
           S  + +   +G++   R  E  E        K  +  ++ EE        XXXXXXXXXX
Sbjct: 87  SQPQETNQINGETSIKRSIELPELKTEVAEGKTVAMEQNSEE---XXXXXXXXXXXXXXX 146

Query: 122 XXXXXXXXXXXALSKEELNKKCEEFIRKMKAGIKLESQQLLVL 157
           XXXXXXXXXX  LS EELNKKCE+FIRKMKAGIK E+QQ +++
Sbjct: 147 XXXXXXXXXXGLLSAEELNKKCEDFIRKMKAGIKFEAQQQILV 185

BLAST of Carg18075-RA vs. TrEMBL
Match: tr|V4UFH1|V4UFH1_9ROSI (Uncharacterized protein OS=Citrus clementina OX=85681 GN=CICLE_v10016749mg PE=4 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 1.0e-09
Identity = 55/177 (31.07%), Postives = 86/177 (48.59%), Query Frame = 0

Query: 2   KLLISASLLSAMVFYSSLLPFLHSFNLYISATAHKNYMFLLCNGLLVFIVRNSGLISCSG 61
           K L+  S+ S +V YSSL+    +  L+   +  KNYMFLLCNG+LVFIV+NSG+I+ S 
Sbjct: 29  KFLLPVSIFSIVVSYSSLIS--AALKLFSGFSIDKNYMFLLCNGILVFIVKNSGVINKSP 88

Query: 62  DRHSSGDSRGAEALEASMAD------GSSKIESKVEDREERGNGLI-------------- 121
           +   +       A++   ++         K++ +V + +      +              
Sbjct: 89  EEKETDLVNAKNAIKEKQSEQQVVKLSEEKVDMEVAEAQPEKRSCVVVADENVVPVAQVD 148

Query: 122 --QKXXXXXXXXXXXXXXXXXXXXXALSKEELNKKCEEFIRKMKAGIKLESQQLLVL 157
             ++                      L+ EELNKKC+EFIRKMK GIK E+QQL+++
Sbjct: 149 GDEEEAQEQECEILITDEDREGGIGLLTTEELNKKCDEFIRKMKEGIKFEAQQLIMV 203

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023530308.16.1e-5998.72uncharacterized protein LOC111792915 [Cucurbita pepo subsp. pepo][more]
XP_022928452.11.2e-5797.48uncharacterized protein LOC111435260 [Cucurbita moschata][more]
XP_011658666.15.6e-3670.37PREDICTED: uncharacterized protein LOC105436067 [Cucumis sativus] >KGN43414.1 hy... [more]
XP_022969273.19.5e-2866.27sodium/potassium/calcium exchanger 1-like [Cucurbita maxima][more]
XP_008462077.23.5e-2263.19PREDICTED: gem-associated protein 2-like [Cucumis melo][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
tr|A0A0A0K349|A0A0A0K349_CUCSA3.7e-3670.37Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G032240 PE=4 SV=1[more]
tr|A0A1S3CG16|A0A1S3CG16_CUCME2.3e-2263.19gem-associated protein 2-like OS=Cucumis melo OX=3656 GN=LOC103500515 PE=4 SV=1[more]
tr|A0A2C9UUD3|A0A2C9UUD3_MANES1.6e-1035.81Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_12G066000 PE=4 SV=... [more]
tr|A0A2C9UVE4|A0A2C9UVE4_MANES3.5e-1050.92Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_12G064700 PE=4 SV=... [more]
tr|V4UFH1|V4UFH1_9ROSI1.0e-0931.07Uncharacterized protein OS=Citrus clementina OX=85681 GN=CICLE_v10016749mg PE=4 ... [more]
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0003674 molecular_function

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Carg18075Carg18075gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg18075-RA.exon.1Carg18075-RA.exon.1exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg18075-RA.CDS.1Carg18075-RA.CDS.1CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Carg18075-RACarg18075-RA-proteinpolypeptide


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 109..125
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 85..108
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 60..128
NoneNo IPR availablePANTHERPTHR34947FAMILY NOT NAMEDcoord: 86..155
coord: 1..70