Carg16694-RA (mRNA) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg16694-RA
Type	mRNA
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	Cytochrome P450, putative
Location	Cucurbita_argyrosperma_scaffold_064 : 641616 .. 643951 (-)
Sequence length	885

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCAATCTCTGCTTTCCTCTGCTCATTTTGGTCCCTTGATTCGGATGAAAAACAAATTTCAACCGCCATGTTCTTGGTCTTCGTCGTCACTTTTGCTCTTTTCTGGTTCCGACCAAAAGTCCGGCGTCCCTCTTTGCCTCCGGGCCCCCGTGGCCTGCCGCTGGTCGGATACCTTCCATTTTTATTGCGAAATGTCCACCGCACATTCGCCGATTTGGCCGAAATCTACGGCCCTGTTTTCAAGGTCAGGATTGGGAACAAGCTGTGCGTCGTTCTTAACTCCCCTTCCTCCATCAATGAAGCCTTCCGCCACCACGAAACTGTCTTCCCCAACCGAGATACCACCGTTTGTGCTCTTCTCTGCAGCTACGACGGCTCCGGCATTGTCTTCACCCAGGACGGCACGATTGGAAGAAGCTGAGGAAAATCTTCGTCCGCAAAATGCTTAGCAGATCATTTCTCGATGCGTCCTATTCTGTGCGAAGACAAGAGGTGAGAAAAGTGATTAAAGGCGTGTTTGAATCGGCCGGAACCCCAATAGACATCGGCAAAGTGGGTTTCCTTGCTACTATGAAATCGGTTTTGGCAATGACATGGGGCGATTCAGGGCGGCTGATCGGAGAGGATGGGATTGATTTGGATGTTAAGTTTAGAGCAGTGATGGATGAACTGGCGGTGCTGCTTGGAACTCCGAATTTGTCGGATATTTTCCCGATATTGGGTCGGTTCGATTTGCAGGGAATTGCGAGTAGAACGAACAAGGCGATGCGTGTTTGTGATGAGATTCTGAATACCGCCATTGAAGAACAGAGGAAGATGGGAGGAAATGGTGTGATAAAGGGAGGATATTTGCAGTTGCTGTTAGAGCTCGAGGACGACGAAGATAATTCAAACCCCATTACAGATGACCAACTCAAGGCCTTGCTATTGGTACGATTTTAACTCGACTTAGTAGAATAAGTTACTAATTTATGGAGTAAATTGAGTAGAATCGGTACTACAAAATAAATAACTAACTTATGGAATAACTATAATTTGTCGGTAGATTTAATATAATAAGTTAATAATGAATAAACTCCAATAGAATTGATAGTAGTTGACAAGGATGGTTGGGAGAAAGTCTAAGATCATTTATAAGTAAGGATATTATGAGGCTTTTTGAAGTCACGAGAGTTTATGCTCAAAATGGACAAACCATTGTGGAGGTTAGTGGTCTTCAACCGAGTTTTGGATGCTAGTTAGTGTTTTCTACGTGTATGTCCACAACTACAACATCAGAAAGAATTGAAAGGTTGGATCGTGTATTTGGGTATGTCTATGGAATTATTAAGGACATGATCATTTGTGTATGGTAACAGGACATGGTCATTGGTGGAACCAAAACAACAGTGACAACAATTGAGTGGGCGATGGCGGAGCTAATGCAGCATCCAAACACAATGAAGAAGGTGAAAGAAGAGCTAAGAGAAGTGGTGGGTTTAGACGCAGCAGCAGAAGAATCTCACATGTCCAAATTAAGCTATCTAAATGCAGTGGTCAAAGAGACGCTTCGTTTACACCCACCTATATTTCTCTTGGTGCCTCGTAGCCTCACCAGCACAATCACCCTTGAGGGCTACTCCATCCCAAAGGGTTCCGCCATGTATTGCAACATTTGGGCCATTCTAAGAGACCCTAAAGTTTGGGAGAACCCCTTAAAGTTCATGCCAGAAAGGTTCTTGAATGAAGGTGGTGGGAAATACGAGCTTGGTGGGAGTGCCATGGAGTTCAGCCCGTTTGGATATGGCAAGAGGTCATGTCCAGGGATCCCTTTGGCTGAAAGGATGTTGATGCTCATATTGGCGTCGTTGTTGCATGCTTTTGAGTGGGAATTGCCTCAACATTCAGTGCTTGATTTCGATGAGAAGTTTGGAGTTGTCAACAAGAAGTTGAACCCTTTGGTTGCTATTCCCACGCCAAGGCTATCCAATTTGAACCTCTATTCGGTTTGAATAATACATTAGTGGTTAGTTTGAGATCGTTATAAGTTCTCGACAACTCGAACCGAAGTAAATAAGTGTGCAAGTAATTTTAAATTTCGTATAAATAAATCATAATGTTAGGTATATGAATATTAGATTGTTGTGATTTGGCTATATGATGATCGAAGTATACAAGCATTATGATCGGACAGAAGTAGCAAGTAGGCTTCAATCTTGTCCCACTATAATAGTTGGATGCAACAATAACTATTTATTTGTAAACACTCCTCTCTCTTCAGCGGTAGGTCCAAATCAACAAGTTGGTGGCAAGATCTACCTAGATGACCCACCCGAGTTTTCACATAAGGAGTAA

mRNA sequence

ATGTCAATCTCTGCTTTCCTCTGCTCATTTTGGTCCCTTGATTCGGATGAAAAACAAATTTCAACCGCCATGTTCTTGGTCTTCGTCGTCACTTTTGCTCTTTTCTGGTTCCGACCAAAAGTCCGGCGTCCCTCTTTGCCTCCGGGCCCCCGTGGCCTGCCGCTGGTCGGATACCTTCCATTTTTATTGCGAAATGTCCACCGCACATTCGCCGATTTGGCCGAAATCTACGGCCCTGTTTTCAAGGTCAGGATTGGGAACAAGCTGTGCGTCGTTCTTAACTCCCCTTCCTCCATCAATGAAGCCTTCCGCCACCACGAAACTGTCTTCCCCAACCGAGATACCACCCTACGACGGCTCCGGCATTGTCTTCACCCAGGACGGCACGATTGGAAGAAGCTGAGGAAAATCTTCGTCCGCAAAATGCTTAGCAGATCATTTCTCGATGCGTCCTATTCTGTGCGAAGACAAGAGGTGAGAAAAGTGATTAAAGGCGTGTTTGAATCGGCCGGAACCCCAATAGACATCGGCAAAGTGGGTTTCCTTGCTACTATGAAATCGGTTTTGGCAATGACATGGGGCGATTCAGGGCGGCTGATCGGAGAGGATGGGATTGATTTGGATGTTAAGTTTAGAGCAGTGATGGATGAACTGGCGGTGCTGCTTGGAACTCCGAATTTGTCGGATATTTTCCCGATATTGGGTCGGTTCGATTTGCAGGGAATTGCGAGTAGAACGAACAAGGCGATGCGTGTTTGTGATGAGATTCTGAATACCGCCATTGAAGAACAGAGGAAGATGGGAGGAAATGCGGTAGGTCCAAATCAACAAGTTGGTGGCAAGATCTACCTAGATGACCCACCCGAGTTTTCACATAAGGAGTAA

Coding sequence (CDS)

ATGTCAATCTCTGCTTTCCTCTGCTCATTTTGGTCCCTTGATTCGGATGAAAAACAAATTTCAACCGCCATGTTCTTGGTCTTCGTCGTCACTTTTGCTCTTTTCTGGTTCCGACCAAAAGTCCGGCGTCCCTCTTTGCCTCCGGGCCCCCGTGGCCTGCCGCTGGTCGGATACCTTCCATTTTTATTGCGAAATGTCCACCGCACATTCGCCGATTTGGCCGAAATCTACGGCCCTGTTTTCAAGGTCAGGATTGGGAACAAGCTGTGCGTCGTTCTTAACTCCCCTTCCTCCATCAATGAAGCCTTCCGCCACCACGAAACTGTCTTCCCCAACCGAGATACCACCCTACGACGGCTCCGGCATTGTCTTCACCCAGGACGGCACGATTGGAAGAAGCTGAGGAAAATCTTCGTCCGCAAAATGCTTAGCAGATCATTTCTCGATGCGTCCTATTCTGTGCGAAGACAAGAGGTGAGAAAAGTGATTAAAGGCGTGTTTGAATCGGCCGGAACCCCAATAGACATCGGCAAAGTGGGTTTCCTTGCTACTATGAAATCGGTTTTGGCAATGACATGGGGCGATTCAGGGCGGCTGATCGGAGAGGATGGGATTGATTTGGATGTTAAGTTTAGAGCAGTGATGGATGAACTGGCGGTGCTGCTTGGAACTCCGAATTTGTCGGATATTTTCCCGATATTGGGTCGGTTCGATTTGCAGGGAATTGCGAGTAGAACGAACAAGGCGATGCGTGTTTGTGATGAGATTCTGAATACCGCCATTGAAGAACAGAGGAAGATGGGAGGAAATGCGGTAGGTCCAAATCAACAAGTTGGTGGCAAGATCTACCTAGATGACCCACCCGAGTTTTCACATAAGGAGTAA

Protein sequence

MSISAFLCSFWSLDSDEKQISTAMFLVFVVTFALFWFRPKVRRPSLPPGPRGLPLVGYLPFLLRNVHRTFADLAEIYGPVFKVRIGNKLCVVLNSPSSINEAFRHHETVFPNRDTTLRRLRHCLHPGRHDWKKLRKIFVRKMLSRSFLDASYSVRRQEVRKVIKGVFESAGTPIDIGKVGFLATMKSVLAMTWGDSGRLIGEDGIDLDVKFRAVMDELAVLLGTPNLSDIFPILGRFDLQGIASRTNKAMRVCDEILNTAIEEQRKMGGNAVGPNQQVGGKIYLDDPPEFSHKE

Homology

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BLAST of Carg16694-RA vs. NCBI nr
Match: XP_023550541.1 (ferruginol synthase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 486.1 bits (1250), Expect = 8.4e-134
Identity = 251/281 (89.32%), Postives = 257/281 (91.46%), Query Frame = 0


Query: 1   MSISAFLCSFWSLDSDE-KQISTAMFLVFVVTFALFWFRPKVRRPSLPPGPRGLPLVGYL 60
           MSISAFLCSFWSLDSDE KQISTA+FLV VVTFALFWFR KVRRPSLPPGPRGLPLVGYL
Sbjct: 1   MSISAFLCSFWSLDSDEVKQISTAIFLVSVVTFALFWFRSKVRRPSLPPGPRGLPLVGYL 60

Query: 61  PFLLRNVHRTFADLAEIYGPVFKVRIGNKLCVVLNSPSSINEAFRHHETVFPNRDTTLRR 120
           PFLLRN+HRTFADLAEIYG VFK+RIGNKLCVVLNSPSSINEAFRHHETVFPNRDTT+  
Sbjct: 61  PFLLRNIHRTFADLAEIYGAVFKLRIGNKLCVVLNSPSSINEAFRHHETVFPNRDTTVCA 120

Query: 121 LRHCLHPGR--------HDWKKLRKIFVRKMLSRSFLDASYSVRRQEVRKVIKGVFESAG 180
           L  C + G          DWKKLRKIFVRKMLS+SFLDASYSVRRQEVRKVIKGVFESAG
Sbjct: 121 LL-CSYDGSGIVFTQDGDDWKKLRKIFVRKMLSKSFLDASYSVRRQEVRKVIKGVFESAG 180

Query: 181 TPIDIGKVGFLATMKSVLAMTWGDSGRLIGEDGIDLDVKFRAVMDELAVLLGTPNLSDIF 240
           TPIDIGKVGFLATMKSVLAMTWGDSGRLIGEDGIDLDVKFRAVMDELAVLLGTPNLSDIF
Sbjct: 181 TPIDIGKVGFLATMKSVLAMTWGDSGRLIGEDGIDLDVKFRAVMDELAVLLGTPNLSDIF 240

Query: 241 PILGRFDLQGIASRTNKAMRVCDEILNTAIEEQRKMGGNAV 273
           PILG FDLQGIASRT KAMRVCDEILNTAIEEQRK GGN V
Sbjct: 241 PILGWFDLQGIASRTKKAMRVCDEILNTAIEEQRKTGGNGV 280

[Close]

BLAST of Carg16694-RA vs. NCBI nr
Match: XP_008437141.1 (PREDICTED: 7-ethoxycoumarin O-deethylase-like [Cucumis melo])

HSP 1 Score: 360.1 bits (923), Expect = 6.9e-96
Identity = 178/274 (64.96%), Postives = 210/274 (76.64%), Query Frame = 0


Query: 7   LCSFWSLDSDE-KQISTAMFLVFVVTFALFWFRPKVRRPSLPPGPRGLPLVGYLPFLLRN 66
           L SFWS+DS++  QIST +  +F+  FAL W R K RRPSLPPGPRGLPLVGYLPFL  N
Sbjct: 3   LISFWSVDSNKLNQISTPILFIFLFIFALLWLRSKFRRPSLPPGPRGLPLVGYLPFLSPN 62

Query: 67  VHRTFADLAEIYGPVFKVRIGNKLCVVLNSPSSINEAFRHHETVFPNRDTTLRRLRH--- 126
           +H  FADLA++YG + K+ +G KLC+VL SPSS+NEA RH ET+F  RDTT+  L     
Sbjct: 63  LHHNFADLAKVYGSILKLHLGTKLCIVLTSPSSVNEALRHQETIFSIRDTTVVALLSSYG 122

Query: 127 ----CLHPGRHDWKKLRKIFVRKMLSRSFLDASYSVRRQEVRKVIKGVFESAGTPIDIGK 186
                     ++WKKLRKIF RKMLS+S LDASYS+RRQEVRKVIKGVFESAGTPIDIGK
Sbjct: 123 GSDIVFTQDENNWKKLRKIFTRKMLSKSNLDASYSLRRQEVRKVIKGVFESAGTPIDIGK 182

Query: 187 VGFLATMKSVLAMTWGDSGRLIGEDGIDLDVKFRAVMDELAVLLGTPNLSDIFPILGRFD 246
           +GF+A +KSV+AMTWG SG LIG DG DLD KFR  +DE+ VLL TPNLSD+FP+LGRFD
Sbjct: 183 LGFVAALKSVMAMTWGGSGGLIGVDGTDLDAKFREAVDEMMVLLATPNLSDLFPVLGRFD 242

Query: 247 LQGIASRTNKAMRVCDEILNTAIEEQRKMGGNAV 273
           LQG+  +  K M V DEI N+AIEEQRK GGN +
Sbjct: 243 LQGVVRKMKKVMCVFDEIFNSAIEEQRKRGGNGM 276

[Close]

BLAST of Carg16694-RA vs. NCBI nr
Match: XP_008437145.1 (PREDICTED: 7-ethoxycoumarin O-deethylase-like [Cucumis melo])

HSP 1 Score: 351.3 bits (900), Expect = 3.2e-93
Identity = 191/297 (64.31%), Postives = 217/297 (73.06%), Query Frame = 0


Query: 1   MSISA-----FLCSFWSLDSDE-KQISTAMFLVFVVTFALFWFRPKVRRPSLPPGPRGLP 60
           MSISA      L SFWS   +E  QIS ++ LVF+V   LF FRPK  RP LPPGP GLP
Sbjct: 32  MSISADLSFSILPSFWSTYFNENNQISISILLVFLV-LTLFRFRPKFWRPCLPPGPCGLP 91

Query: 61  LVGYLPFLLRNVHRTFADLAEIYGPVFKVRIGNKLCVVLNSPSSINEAFRHHETVFPNRD 120
           LVGYLPFL  N H     LA+IYGPV+K+R+G KLC+VLNSP+S+ EAF H ET+FPNRD
Sbjct: 92  LVGYLPFLSGNPHHKLTHLAKIYGPVYKLRLGPKLCIVLNSPASVKEAFHHQETLFPNRD 151

Query: 121 TTLRRLRH-------CLHPGRHDWKKLRKIFVRKMLSRSFLDASYSVRRQEVRKVIKGVF 180
           TT+  L                DWKKLRKIF RKM+S+S LDASY VRRQEVRKVIKGVF
Sbjct: 152 TTVCALLSGYGGSGIVFAQDEGDWKKLRKIFTRKMISKSNLDASYFVRRQEVRKVIKGVF 211

Query: 181 ESAGTPIDIGKVGFLATMKSVLAMTWGDSGRLIGEDGIDLDVKFRAVMDELAVLLGTPNL 240
           ES GT IDIGKV F ATMKSVLAMTWGDSGRL+GEDG+ L+ KFR +MDEL VLLG+PN+
Sbjct: 212 ESVGTQIDIGKVSFHATMKSVLAMTWGDSGRLMGEDGVGLEEKFREMMDELMVLLGSPNV 271

Query: 241 SDIFPILGRFDLQGIASRTNKAMRVCDEILNTAIEEQRKMGGNAVGPNQQVGGKIYL 285
           SD+FP+LGRFDLQGI  RT K M V D ILN +IEEQRKMGG   G  +  GG + L
Sbjct: 272 SDVFPVLGRFDLQGIGRRTKKVMGVLDGILNFSIEEQRKMGGKGNGEGK--GGYLQL 325

[Close]

BLAST of Carg16694-RA vs. NCBI nr
Match: KGN50204.1 (hypothetical protein Csa_5G158600 [Cucumis sativus])

HSP 1 Score: 347.1 bits (889), Expect = 6.1e-92
Identity = 175/249 (70.28%), Postives = 195/249 (78.31%), Query Frame = 0


Query: 32  FALFWFRPKVRRPSLPPGPRGLPLVGYLPFLLRNVHRTFADLAEIYGPVFKVRIGNKLCV 91
           FAL W R K RRPSLPPGPRGLPLVGYLPFL  N H     LA+IYGPVFK+R+G KLCV
Sbjct: 2   FALLWLRSKFRRPSLPPGPRGLPLVGYLPFLSGNPHHKLTHLAKIYGPVFKLRLGPKLCV 61

Query: 92  VLNSPSSINEAFRHHETVFPNRDTTLRRLRH-------CLHPGRHDWKKLRKIFVRKMLS 151
           VL SP+ I EAF H ET+FPNR+TT+  L                DWKKLRKIFVRKM+S
Sbjct: 62  VLTSPAFIKEAFHHQETLFPNRNTTVCALLSSYGCSGIVFAQDGEDWKKLRKIFVRKMIS 121

Query: 152 RSFLDASYSVRRQEVRKVIKGVFESAGTPIDIGKVGFLATMKSVLAMTWGDSGRLIGEDG 211
           +S LDASY VRRQEVRKVIKGVFES GT IDIGKV FLATMKSV+AMTWGDSGR++GEDG
Sbjct: 122 KSNLDASYCVRRQEVRKVIKGVFESVGTQIDIGKVSFLATMKSVVAMTWGDSGRVMGEDG 181

Query: 212 IDLDVKFRAVMDELAVLLGTPNLSDIFPILGRFDLQGIASRTNKAMRVCDEILNTAIEEQ 271
           + L+VKFR +MDEL VLLG+ N+SD+FP+LGRFDLQGI  RT K M V D ILN+AIEEQ
Sbjct: 182 VGLEVKFREMMDELVVLLGSRNVSDVFPVLGRFDLQGIGRRTKKVMGVLDGILNSAIEEQ 241

Query: 272 RKMGGNAVG 274
           RKMGGN  G
Sbjct: 242 RKMGGNGGG 250

[Close]

BLAST of Carg16694-RA vs. NCBI nr
Match: XP_004147609.1 (PREDICTED: 7-ethoxycoumarin O-deethylase-like [Cucumis sativus])

HSP 1 Score: 342.0 bits (876), Expect = 2.0e-90
Identity = 172/245 (70.20%), Postives = 192/245 (78.37%), Query Frame = 0


Query: 36  WFRPKVRRPSLPPGPRGLPLVGYLPFLLRNVHRTFADLAEIYGPVFKVRIGNKLCVVLNS 95
           W R K RRPSLPPGPRGLPLVGYLPFL  N H     LA+IYGPVFK+R+G KLCVVL S
Sbjct: 39  WLRSKFRRPSLPPGPRGLPLVGYLPFLSGNPHHKLTHLAKIYGPVFKLRLGPKLCVVLTS 98

Query: 96  PSSINEAFRHHETVFPNRDTTLRRLRH-------CLHPGRHDWKKLRKIFVRKMLSRSFL 155
           P+ I EAF H ET+FPNR+TT+  L                DWKKLRKIFVRKM+S+S L
Sbjct: 99  PAFIKEAFHHQETLFPNRNTTVCALLSSYGCSGIVFAQDGEDWKKLRKIFVRKMISKSNL 158

Query: 156 DASYSVRRQEVRKVIKGVFESAGTPIDIGKVGFLATMKSVLAMTWGDSGRLIGEDGIDLD 215
           DASY VRRQEVRKVIKGVFES GT IDIGKV FLATMKSV+AMTWGDSGR++GEDG+ L+
Sbjct: 159 DASYCVRRQEVRKVIKGVFESVGTQIDIGKVSFLATMKSVVAMTWGDSGRVMGEDGVGLE 218

Query: 216 VKFRAVMDELAVLLGTPNLSDIFPILGRFDLQGIASRTNKAMRVCDEILNTAIEEQRKMG 274
           VKFR +MDEL VLLG+ N+SD+FP+LGRFDLQGI  RT K M V D ILN+AIEEQRKMG
Sbjct: 219 VKFREMMDELVVLLGSRNVSDVFPVLGRFDLQGIGRRTKKVMGVLDGILNSAIEEQRKMG 278

[Close]

BLAST of Carg16694-RA vs. TAIR10
Match: AT4G12300.1 (cytochrome P450, family 706, subfamily A, polypeptide 4)

HSP 1 Score: 181.4 bits (459), Expect = 8.0e-46
Identity = 98/254 (38.58%), Postives = 149/254 (58.66%), Query Frame = 0


Query: 26  LVFVVTFALFWF-RPKVRRPSLPPGPRGLPLVGYLPFLLRNVHRTFADLAEIYGPVFKVR 85
           ++    F++ W+   +  +PSLPPGPRGLP+VG LPFL  ++H  FA+LA+ +GP+FK+ 
Sbjct: 21  VILTTVFSILWYIFKRSPQPSLPPGPRGLPIVGNLPFLDPDLHTYFANLAQSHGPIFKLN 80

Query: 86  IGNKLCVVLNSPSSINEAFRHHETVFPNRD-------TTLRRLRHCLHPGRHDWKKLRKI 145
           +G+KL +V+NSPS   E  +  +  F NRD        T   +     P   +W++LRKI
Sbjct: 81  LGSKLTIVVNSPSLAREILKDQDINFSNRDVPLTGRAATYGGIDIVWTPYGAEWRQLRKI 140

Query: 146 FVRKMLSRSFLDASYSVRRQEVRKVIKGVFESA--GTPIDIGKVGFLATMKSVLAMTWGD 205
            V K+LSR  LD+ Y +RR+EVR+  + ++E     +P+ +G   FL  M   + M WG 
Sbjct: 141 CVLKLLSRKTLDSFYELRRKEVRERTRYLYEQGRKQSPVKVGDQLFLTMMNLTMNMLWG- 200

Query: 206 SGRLIGEDGIDLDVKFRAVMDELAVLLGTPNLSDIFPILGRFDLQGIASRTNKAMRVCDE 265
            G +  E+   +  +F+ V+ E+  LL  PN+SD FP L RFDLQG+  R     R  D 
Sbjct: 201 -GSVKAEEMESVGTEFKGVISEITRLLSEPNVSDFFPWLARFDLQGLVKRMGVCARELDA 260

Query: 266 ILNTAIEEQRKMGG 270
           +L+ AIE+ + + G
Sbjct: 261 VLDRAIEQMKPLRG 272

[Close]

BLAST of Carg16694-RA vs. TAIR10
Match: AT4G12310.1 (cytochrome P450, family 706, subfamily A, polypeptide 5)

HSP 1 Score: 180.3 bits (456), Expect = 1.8e-45
Identity = 95/252 (37.70%), Postives = 150/252 (59.52%), Query Frame = 0


Query: 26  LVFVVTFALFWF-RPKVRRPSLPPGPRGLPLVGYLPFLLRNVHRTFADLAEIYGPVFKVR 85
           L+   TF++ W+   +  +P LPPGPRGLP+VG LPFL  ++H  F  LA+ +GP+FK+ 
Sbjct: 23  LILTATFSILWYIFKRSPQPPLPPGPRGLPIVGNLPFLDPDLHTYFTKLAQSHGPIFKLN 82

Query: 86  IGNKLCVVLNSPSSINEAFRHHETVFPNRDTTLRR-------LRHCLHPGRHDWKKLRKI 145
           +G+KL VV+NSPS  +E  +  +  F N D  L         L     P   +W+ LRK+
Sbjct: 83  LGSKLTVVVNSPSLASEILKDQDINFSNHDVPLTARAVTYGGLDLVWLPYGAEWRMLRKV 142

Query: 146 FVRKMLSRSFLDASYSVRRQEVRKVIKGVFESA--GTPIDIGKVGFLATMKSVLAMTWGD 205
              K+ SR  LD+ Y +RR+E+R+  + +++     +P+++G+  FL  M  ++ M WG 
Sbjct: 143 CAAKLFSRKTLDSFYELRRKEIRERTRCLYQKGLEKSPVNVGEQLFLTMMNLMMNMLWG- 202

Query: 206 SGRLIGEDGIDLDVKFRAVMDELAVLLGTPNLSDIFPILGRFDLQGIASRTNKAMRVCDE 265
            G +  ED   +  +F+ V+ E+  LLG PN+SD FP+L RFDLQG+  + +   R  D 
Sbjct: 203 -GSVKAEDMESVGTEFKGVISEITRLLGVPNVSDFFPMLARFDLQGLVKKMHLYARDLDA 262

Query: 266 ILNTAIEEQRKM 268
           IL+ AIE+ +++
Sbjct: 263 ILDRAIEQMQRL 272

[Close]

BLAST of Carg16694-RA vs. TAIR10
Match: AT4G12320.1 (cytochrome P450, family 706, subfamily A, polypeptide 6)

HSP 1 Score: 175.3 bits (443), Expect = 5.7e-44
Identity = 94/250 (37.60%), Postives = 146/250 (58.40%), Query Frame = 0


Query: 26  LVFVVTFALFWFR-PKVRRPSLPPGPRGLPLVGYLPFLLRNVHRTFADLAEIYGPVFKVR 85
           L+ +  F++ W+   +  +P LPPGPRGLP+VG LPFL  ++H  F  LAE YGP+FK+ 
Sbjct: 21  LILIAIFSILWYLFKRSPQPHLPPGPRGLPIVGNLPFLDPDLHTYFTKLAESYGPIFKLN 80

Query: 86  IGNKLCVVLNSPSSINEAFRHHETVFPNRDTTLRR-------LRHCLHPGRHDWKKLRKI 145
           +G+KL VV+N+PS   E  +  +  F N D  L         L     P   +W+ LRK+
Sbjct: 81  LGSKLTVVVNTPSLAREILKDQDINFSNHDVPLTARAVTYGGLDLVWLPYGAEWRMLRKV 140

Query: 146 FVRKMLSRSFLDASYSVRRQEVRKVIKGVFESA--GTPIDIGKVGFLATMKSVLAMTWGD 205
            V K+LS   L++ Y +RR+E+R+  + +++     +P+++G+  FL  M   + M WG 
Sbjct: 141 CVLKLLSHRTLNSFYELRRKEIRERTRYLYQKGQEESPVNVGEQVFLTMMNLTMNMLWG- 200

Query: 206 SGRLIGEDGIDLDVKFRAVMDELAVLLGTPNLSDIFPILGRFDLQGIASRTNKAMRVCDE 265
            G +  E+   +  +F+ V+ E+  LLG PN+SD FP L RFDLQG+  + +   R  D 
Sbjct: 201 -GSVKAEEMESVGTEFKEVISEITRLLGEPNVSDFFPRLARFDLQGLVKKMHVCARELDA 260

[Close]

BLAST of Carg16694-RA vs. TAIR10
Match: AT4G12330.1 (cytochrome P450, family 706, subfamily A, polypeptide 7)

HSP 1 Score: 152.9 bits (385), Expect = 3.0e-37
Identity = 88/264 (33.33%), Postives = 147/264 (55.68%), Query Frame = 0


Query: 26  LVFVVTFALFWF-----RPKVRRPSLPPGPRGLPLVGYLPFLLRNVHRTFADLAEIYGPV 85
           L  +V  ++FW+     + K   P LPPGPRGLP+VG LPFL   +H  F  LA+ +GPV
Sbjct: 21  LATIVISSVFWYIWVYVKSKRLFPPLPPGPRGLPIVGNLPFLHPELHTYFHSLAQKHGPV 80

Query: 86  FKVRIGNKLCVVLNSPSSINEAFRHHETVFPNRD-------TTLRRLRHCLHPGRHDWKK 145
           FK+ +G KL +V+ S  +  +  R ++ +F N D       +T   +     P   +W  
Sbjct: 81  FKLWLGAKLTIVITSSEATRDILRTNDVIFANDDVPVAGSLSTYGGVDIVWSPYGPEWPM 140

Query: 146 LRKIFVRKMLSRSFLDAS--YSVRRQEVRKVIKGVFE--SAGTPIDIGKVGFLATMKSVL 205
           LRKI + KMLS + LD++   ++RRQE R+ ++ + +   AG  +++G+  F+  +  V 
Sbjct: 141 LRKICINKMLSNATLDSNSFSALRRQETRRTVRYLADRARAGLAVNVGEQIFVTILNVVT 200

Query: 206 AMTWGDSGRLIGEDGIDLDVKFRAVMDELAVLLGTPNLSDIFPILGRFDLQGIASRTNKA 265
            M WG++     E+   +  +F  ++ E+  ++G PN+SD FP+L RFDLQG+A R  ++
Sbjct: 201 QMLWGET-VADDEEREKVGAEFLELITEIIDVVGKPNVSDFFPVLSRFDLQGLAKRVRRS 260

Query: 266 MRVCDEILNTAIEEQRKMGGNAVG 274
            +  D + +  I ++  M   + G
Sbjct: 261 AQRMDRMFDRIISQRMGMDKGSKG 283

[Close]

BLAST of Carg16694-RA vs. TAIR10
Match: AT5G44620.1 (cytochrome P450, family 706, subfamily A, polypeptide 3)

HSP 1 Score: 149.8 bits (377), Expect = 2.6e-36
Identity = 86/265 (32.45%), Postives = 143/265 (53.96%), Query Frame = 0


Query: 15  SDEKQISTAMFLVFVVTFA-LFWFRPKVRR--PSLPPGPRGLPLVGYLPFLLRNVHRTFA 74
           S + Q+   + ++ + T     W   K +R  P LPPGP GLP++G LPFL   +H  F 
Sbjct: 12  SRKDQLDYGLTVIVISTLCWCLWLYAKCKRRSPPLPPGPWGLPIIGNLPFLQPELHTYFQ 71

Query: 75  DLAEIYGPVFKVRIGNKLCVVLNSPSSINEAFRHHETVFPNRD-------TTLRRLRHCL 134
            LA+ +GP+FK+ +G KL +V+ S     E  + ++ +F N D        T        
Sbjct: 72  GLAKKHGPIFKLWLGAKLTIVVTSSEVAQEILKTNDIIFANHDVPAVGPVNTYGGTEIIW 131

Query: 135 HPGRHDWKKLRKIFVRKMLSRSFLDASYSVRRQEVRKVIKGVFESA--GTPIDIGKVGFL 194
            P    W+ LRK+ V ++L  + LD+S  +RR+E R+ ++ + + A  G+P+++G+  FL
Sbjct: 132 SPYGPKWRMLRKLCVNRILRNAMLDSSTDLRRRETRQTVRYLADQARVGSPVNLGEQIFL 191

Query: 195 ATMKSVLAMTWGDSGRLIGEDGIDLDVKFRAVMDELAVLLGTPNLSDIFPILGRFDLQGI 254
             +  V  M WG + +   E+   +  +F  V+ E+  LL  PN+SD FP+L RFDLQG+
Sbjct: 192 MMLNVVTQMLWGTTVK--EEEREVVGAEFLEVIREMNDLLLVPNISDFFPVLSRFDLQGL 251

Query: 255 ASRTNKAMRVCDEILNTAIEEQRKM 268
           A R  +  +  D++ +  I ++  M
Sbjct: 252 AKRMRRPAQRMDQMFDRIINQRLGM 274

[Close]

BLAST of Carg16694-RA vs. Swiss-Prot
Match: sp|S4UX02|CYPH1_SALMI (Ferruginol synthase OS=Salvia miltiorrhiza OX=226208 GN=CYP76AH1 PE=1 SV=1)

HSP 1 Score: 122.1 bits (305), Expect = 1.0e-26
Identity = 75/260 (28.85%), Postives = 130/260 (50.00%), Query Frame = 0


Query: 20  ISTAMFLVFVVTFALFWFRPKVRRPSLPPGPRGLPLVGYLPFLLRNVHRTFADLAEIYGP 79
           ++   F+   +TF  F      RR +LPPGP   P+VG +  L  N H+ FA L++ YGP
Sbjct: 7   LAALFFIAATITFLSF-----RRRRNLPPGPFPYPIVGNMLQLGANPHQVFAKLSKRYGP 66

Query: 80  VFKVRIGNKLCVVLNSPSSINEAFRHHETVFPNRDTTLRRLRHCLH--------PGRHDW 139
           +  + +G+   V+++SP    E    H  VF  R T  + +  C H        P   +W
Sbjct: 67  LMSIHLGSLYTVIVSSPEMAKEILHRHGQVFSGR-TIAQAVHACDHDKISMGFLPVASEW 126

Query: 140 KKLRKIFVRKMLSRSFLDASYSVRRQEVRKVIKGV--FESAGTPIDIGKVGFLATMKSVL 199
           + +RKI   +M S   ++AS  +RRQ++++++  V     +G  +DI +  F+ T+  + 
Sbjct: 127 RDMRKICKEQMFSNQSMEASQGLRRQKLQQLLDHVQKCSDSGRAVDIREAAFITTLNLMS 186

Query: 200 AMTWGDSGRLIGEDGIDLDVKFRAVMDELAVLLGTPNLSDIFPILGRFDLQGIASRTN-- 259
           A  +        E      ++F+ +++ +A ++G PN +D FPIL  FD QG+  R +  
Sbjct: 187 ATLFSSQAT---EFDSKATMEFKEIIEGVATIVGVPNFADYFPILRPFDPQGVKRRADVF 246

Query: 260 --KAMRVCDEILNTAIEEQR 266
             K +   +  LN  +E +R
Sbjct: 247 FGKLLAKIEGYLNERLESKR 257

[Close]

BLAST of Carg16694-RA vs. Swiss-Prot
Match: sp|H2DH18|C7A12_PANGI (Cytochrome P450 CYP736A12 OS=Panax ginseng OX=4054 PE=2 SV=1)

HSP 1 Score: 113.6 bits (283), Expect = 3.7e-24
Identity = 78/283 (27.56%), Postives = 137/283 (48.41%), Query Frame = 0


Query: 26  LVFVVTFALFWF-----RPKVRRPSLPPGPRGLPLVGYLPFLLRNVHRTFADLAEIYGPV 85
           L+FV+  AL W+      P  R   LPPGPRGLP++G L  L    HRT   LA+ YGP+
Sbjct: 8   LLFVLLGALSWWILPIISPLKRHHKLPPGPRGLPIIGSLHTLGALPHRTLQTLAKKYGPI 67

Query: 86  FKVRIGNKLCVVLNSPSSINEAFRHHETVFPNRDTTLRRLRHCLHP---------GRHDW 145
             +R+G+   +V++SP +     + H+ +F +R   L+   +  +          G H W
Sbjct: 68  MSMRLGSVPTIVVSSPQAAELFLKTHDNIFASR-PKLQAAEYMSYGTMGMSFTAYGPH-W 127

Query: 146 KKLRKIFVRKMLSRSFLDASYSVRRQEVRKVIKGVFESAGTPIDIGKVGFLATMKSVLAM 205
           + +RK  V ++L+ + +++   +RR+E+  V+K + E++     +     +A +  +  M
Sbjct: 128 RNIRKFVVLELLTPAKINSFVGMRREELGTVVKSIKEASAANEVVDLSAKVANI--IENM 187

Query: 206 TWGDSGRLIGEDGIDLDVKFRAVMDELAVLLGTPNLSDIFPILGRFDLQGIASRTNKAMR 265
           T+    RL+     D     + +M+E   L G  N++D  P LG  D+QG+  +     +
Sbjct: 188 TY----RLLLGRTKDDRYDLKGIMNEALTLAGRFNIADFVPFLGPLDIQGLTRQFKDTGK 247

Query: 266 VCDEILNTAIEEQRKMGGNAVGPNQQVGGKIYLDDPPEFSHKE 295
             D+IL   I+E  +   N       +   + L + P  +H E
Sbjct: 248 RLDKILEFIIDEHEQNSSNGNASGDFIDDMLSLKNKPSNTHDE 282

[Close]

BLAST of Carg16694-RA vs. Swiss-Prot
Match: sp|Q42600|C84A1_ARATH (Cytochrome P450 84A1 OS=Arabidopsis thaliana OX=3702 GN=CYP84A1 PE=1 SV=1)

HSP 1 Score: 111.7 bits (278), Expect = 1.4e-23
Identity = 78/257 (30.35%), Postives = 123/257 (47.86%), Query Frame = 0


Query: 22  TAMFLVFVVTFALFWFRPKVRRPSLPPGPRGLPLVGYLPFLLRNVHRTFADLAEIYGPVF 81
           T   ++ V  F    F  + RRP  PPGPRG P++G +  + +  HR  A+LA+ YG + 
Sbjct: 16  TTSLVIVVSLFIFISFITRRRRPPYPPGPRGWPIIGNMLMMDQLTHRGLANLAKKYGGLC 75

Query: 82  KVRIGNKLCVVLNSPSSINEAFRHHETVFPNRDTTL--------RRLRHCLHPGRHDWKK 141
            +R+G      ++SP    +  +  ++VF NR  T+        R      H G   W++
Sbjct: 76  HLRMGFLHMYAVSSPEVARQVLQVQDSVFSNRPATIAISYLTYDRADMAFAHYGPF-WRQ 135

Query: 142 LRKIFVRKMLSRSFLDASYSVRRQEVRKVIKGVFESAGTPIDIGKVGFLATMKSVLAMTW 201
           +RK+ V K+ SR   ++  SV R EV K+++ V  + G PI++G+  F  T        +
Sbjct: 136 MRKVCVMKVFSRKRAESWASV-RDEVDKMVRSVSCNVGKPINVGEQIFALTRNITYRAAF 195

Query: 202 GDSGRLIGEDGIDLDVKFRAVMDELAVLLGTPNLSDIFPILGRFDLQGIASRTNKAMRVC 261
           G +     E G D   +F  ++ E + L G  N++D  P  G  D QGI  R  KA    
Sbjct: 196 GSA----CEKGQD---EFIRILQEFSKLFGAFNVADFIPYFGWIDPQGINKRLVKARNDL 255

Query: 262 DEILNTAIEEQRKMGGN 271
           D  ++  I+E  K   N
Sbjct: 256 DGFIDDIIDEHMKKKEN 263

[Close]

BLAST of Carg16694-RA vs. Swiss-Prot
Match: sp|Q6WNQ9|C81E9_MEDTR (Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 PE=1 SV=1)

HSP 1 Score: 110.2 bits (274), Expect = 4.1e-23
Identity = 80/263 (30.42%), Postives = 127/263 (48.29%), Query Frame = 0


Query: 26  LVFVVTFALFWFRPKVRRPSLPPGPRGLPLVGYLPFLLRNVHRTFADLAEIYGPVFKVRI 85
           L F++T  +       R  +LPPGP  +P++G L  L   +HRTF  L++ YG +F +  
Sbjct: 10  LSFIITIKILLKITSRRLKNLPPGPPTIPIIGNLHHLKHPLHRTFTTLSQTYGDIFSLWF 69

Query: 86  GNKLCVVLNSPSSINEAFRHHETVFPNRD------------TTLRRLRHCLHPGRHDWKK 145
           G++L VV++SPS  +E F  ++ +  NR             TTL    +    G H W+ 
Sbjct: 70  GSRLVVVVSSPSLAHECFTKNDIILANRPRFLTGKYIFYNYTTLGSASY----GDH-WRN 129

Query: 146 LRKIFVRKMLSRSFLDASYSVRRQEVRKVIKG-----VFESAG-TPIDIGKVGFLATMKS 205
           LR+I    +LS + L++   VRR E  ++I+      V E  G T +++       T  +
Sbjct: 130 LRRITTIDVLSNNRLNSFLGVRRDETNRLIQKLLKDVVSEGFGFTKVELRPRLTEMTFNA 189

Query: 206 VLAMTWGDSGRLIGEDGIDLDV----KFRAVMDELAVLLGTPNLSDIFPILGRFDLQGIA 265
           ++ M  G   R  G+DG   DV    +FR ++ E+  LLG  N  D  P+L   DL  + 
Sbjct: 190 MMRMISGK--RYYGDDGDVSDVEEAKQFREIISEMMSLLGANNKGDFLPLLRVVDLDNLE 249

Query: 266 SRTNKAMRVCDEILNTAIEEQRK 267
            R  +  +  +  L   IEE R+
Sbjct: 250 KRCKRIAKRSNAFLEGLIEEHRR 265

[Close]

BLAST of Carg16694-RA vs. Swiss-Prot
Match: sp|F4JW83|C84A4_ARATH (Cytochrome P450 84A4 OS=Arabidopsis thaliana OX=3702 GN=CYP84A4 PE=1 SV=1)

HSP 1 Score: 109.0 bits (271), Expect = 9.1e-23
Identity = 74/256 (28.91%), Postives = 126/256 (49.22%), Query Frame = 0


Query: 22  TAMFLVFVVTFALFWF-----RPKVRRPSLPPGPRGLPLVGYLPFLLRNVHRTFADLAEI 81
           T M L+ +V   LF F     R ++     PPGP+GLP++G +  + +  HR  A L+ I
Sbjct: 3   TLMTLIVLVPLLLFLFPHLLLRRQMLLKPYPPGPKGLPVIGNILMMNQFNHRGLAKLSRI 62

Query: 82  YGPVFKVRIGNKLCVVLNSPSSINEAFRHHETVFPNRDTTLRRLRHCLHPGRH------- 141
           YG +  +R+G     V++SP    +  +  + VF NR TT+  +R+  + G         
Sbjct: 63  YGGLLHLRLGFSHIFVVSSPDIARQVLQVQDHVFSNRPTTI-AIRYLTYGGSDLAFCNYG 122

Query: 142 -DWKKLRKIFVRKMLSRSFLDASYSVRRQEVRKVIKGVFESAGTPIDIGKVGFLATMKSV 201
             W+++RK++V  + SR   ++  SV  +EV K ++ V  + G P++I K+ F  +    
Sbjct: 123 PFWRRMRKLYVMMLFSRKRAESWVSV-DEEVHKSVRLVASNVGKPLNICKLAFSLSRDIT 182

Query: 202 LAMTWGDSGRLIGEDGIDLDVKFRAVMDELAVLLGTPNLSDIFPI-LGRFDLQGIASRTN 261
               +G S     E  +D   +F  ++ E + L G  N++D  P  L   D QGI  R  
Sbjct: 183 FRAAFGSSSSTSDESRLD---EFLEIIQEFSKLFGEFNVADYVPSWLSWIDPQGINGRVE 242

Query: 262 KAMRVCDEILNTAIEE 264
           KA +  D  + + I++
Sbjct: 243 KARKSLDGFIESVIDD 253

[Close]

BLAST of Carg16694-RA vs. TrEMBL
Match: tr|A0A1S3ATA1|A0A1S3ATA1_CUCME (7-ethoxycoumarin O-deethylase-like OS=Cucumis melo OX=3656 GN=LOC103482652 PE=3 SV=1)

HSP 1 Score: 360.1 bits (923), Expect = 4.6e-96
Identity = 178/274 (64.96%), Postives = 210/274 (76.64%), Query Frame = 0


Query: 7   LCSFWSLDSDE-KQISTAMFLVFVVTFALFWFRPKVRRPSLPPGPRGLPLVGYLPFLLRN 66
           L SFWS+DS++  QIST +  +F+  FAL W R K RRPSLPPGPRGLPLVGYLPFL  N
Sbjct: 3   LISFWSVDSNKLNQISTPILFIFLFIFALLWLRSKFRRPSLPPGPRGLPLVGYLPFLSPN 62

Query: 67  VHRTFADLAEIYGPVFKVRIGNKLCVVLNSPSSINEAFRHHETVFPNRDTTLRRLRH--- 126
           +H  FADLA++YG + K+ +G KLC+VL SPSS+NEA RH ET+F  RDTT+  L     
Sbjct: 63  LHHNFADLAKVYGSILKLHLGTKLCIVLTSPSSVNEALRHQETIFSIRDTTVVALLSSYG 122

Query: 127 ----CLHPGRHDWKKLRKIFVRKMLSRSFLDASYSVRRQEVRKVIKGVFESAGTPIDIGK 186
                     ++WKKLRKIF RKMLS+S LDASYS+RRQEVRKVIKGVFESAGTPIDIGK
Sbjct: 123 GSDIVFTQDENNWKKLRKIFTRKMLSKSNLDASYSLRRQEVRKVIKGVFESAGTPIDIGK 182

Query: 187 VGFLATMKSVLAMTWGDSGRLIGEDGIDLDVKFRAVMDELAVLLGTPNLSDIFPILGRFD 246
           +GF+A +KSV+AMTWG SG LIG DG DLD KFR  +DE+ VLL TPNLSD+FP+LGRFD
Sbjct: 183 LGFVAALKSVMAMTWGGSGGLIGVDGTDLDAKFREAVDEMMVLLATPNLSDLFPVLGRFD 242

Query: 247 LQGIASRTNKAMRVCDEILNTAIEEQRKMGGNAV 273
           LQG+  +  K M V DEI N+AIEEQRK GGN +
Sbjct: 243 LQGVVRKMKKVMCVFDEIFNSAIEEQRKRGGNGM 276

[Close]

BLAST of Carg16694-RA vs. TrEMBL
Match: tr|A0A1S3ATF6|A0A1S3ATF6_CUCME (7-ethoxycoumarin O-deethylase-like OS=Cucumis melo OX=3656 GN=LOC103482655 PE=3 SV=1)

HSP 1 Score: 351.3 bits (900), Expect = 2.1e-93
Identity = 191/297 (64.31%), Postives = 217/297 (73.06%), Query Frame = 0


Query: 1   MSISA-----FLCSFWSLDSDE-KQISTAMFLVFVVTFALFWFRPKVRRPSLPPGPRGLP 60
           MSISA      L SFWS   +E  QIS ++ LVF+V   LF FRPK  RP LPPGP GLP
Sbjct: 32  MSISADLSFSILPSFWSTYFNENNQISISILLVFLV-LTLFRFRPKFWRPCLPPGPCGLP 91

Query: 61  LVGYLPFLLRNVHRTFADLAEIYGPVFKVRIGNKLCVVLNSPSSINEAFRHHETVFPNRD 120
           LVGYLPFL  N H     LA+IYGPV+K+R+G KLC+VLNSP+S+ EAF H ET+FPNRD
Sbjct: 92  LVGYLPFLSGNPHHKLTHLAKIYGPVYKLRLGPKLCIVLNSPASVKEAFHHQETLFPNRD 151

Query: 121 TTLRRLRH-------CLHPGRHDWKKLRKIFVRKMLSRSFLDASYSVRRQEVRKVIKGVF 180
           TT+  L                DWKKLRKIF RKM+S+S LDASY VRRQEVRKVIKGVF
Sbjct: 152 TTVCALLSGYGGSGIVFAQDEGDWKKLRKIFTRKMISKSNLDASYFVRRQEVRKVIKGVF 211

Query: 181 ESAGTPIDIGKVGFLATMKSVLAMTWGDSGRLIGEDGIDLDVKFRAVMDELAVLLGTPNL 240
           ES GT IDIGKV F ATMKSVLAMTWGDSGRL+GEDG+ L+ KFR +MDEL VLLG+PN+
Sbjct: 212 ESVGTQIDIGKVSFHATMKSVLAMTWGDSGRLMGEDGVGLEEKFREMMDELMVLLGSPNV 271

Query: 241 SDIFPILGRFDLQGIASRTNKAMRVCDEILNTAIEEQRKMGGNAVGPNQQVGGKIYL 285
           SD+FP+LGRFDLQGI  RT K M V D ILN +IEEQRKMGG   G  +  GG + L
Sbjct: 272 SDVFPVLGRFDLQGIGRRTKKVMGVLDGILNFSIEEQRKMGGKGNGEGK--GGYLQL 325

[Close]

BLAST of Carg16694-RA vs. TrEMBL
Match: tr|A0A0A0KMT1|A0A0A0KMT1_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G158600 PE=4 SV=1)

HSP 1 Score: 347.1 bits (889), Expect = 4.0e-92
Identity = 175/249 (70.28%), Postives = 195/249 (78.31%), Query Frame = 0


Query: 32  FALFWFRPKVRRPSLPPGPRGLPLVGYLPFLLRNVHRTFADLAEIYGPVFKVRIGNKLCV 91
           FAL W R K RRPSLPPGPRGLPLVGYLPFL  N H     LA+IYGPVFK+R+G KLCV
Sbjct: 2   FALLWLRSKFRRPSLPPGPRGLPLVGYLPFLSGNPHHKLTHLAKIYGPVFKLRLGPKLCV 61

Query: 92  VLNSPSSINEAFRHHETVFPNRDTTLRRLRH-------CLHPGRHDWKKLRKIFVRKMLS 151
           VL SP+ I EAF H ET+FPNR+TT+  L                DWKKLRKIFVRKM+S
Sbjct: 62  VLTSPAFIKEAFHHQETLFPNRNTTVCALLSSYGCSGIVFAQDGEDWKKLRKIFVRKMIS 121

Query: 152 RSFLDASYSVRRQEVRKVIKGVFESAGTPIDIGKVGFLATMKSVLAMTWGDSGRLIGEDG 211
           +S LDASY VRRQEVRKVIKGVFES GT IDIGKV FLATMKSV+AMTWGDSGR++GEDG
Sbjct: 122 KSNLDASYCVRRQEVRKVIKGVFESVGTQIDIGKVSFLATMKSVVAMTWGDSGRVMGEDG 181

Query: 212 IDLDVKFRAVMDELAVLLGTPNLSDIFPILGRFDLQGIASRTNKAMRVCDEILNTAIEEQ 271
           + L+VKFR +MDEL VLLG+ N+SD+FP+LGRFDLQGI  RT K M V D ILN+AIEEQ
Sbjct: 182 VGLEVKFREMMDELVVLLGSRNVSDVFPVLGRFDLQGIGRRTKKVMGVLDGILNSAIEEQ 241

Query: 272 RKMGGNAVG 274
           RKMGGN  G
Sbjct: 242 RKMGGNGGG 250

[Close]

BLAST of Carg16694-RA vs. TrEMBL
Match: tr|A0A1S3ATW0|A0A1S3ATW0_CUCME (flavonoid 3'-monooxygenase-like OS=Cucumis melo OX=3656 GN=LOC103482653 PE=3 SV=1)

HSP 1 Score: 328.2 bits (840), Expect = 1.9e-86
Identity = 166/262 (63.36%), Postives = 192/262 (73.28%), Query Frame = 0


Query: 19  QISTAMFLVFVVTFALFWFRPKVRRPSLPPGPRGLPLVGYLPFLLRNVHRTFADLAEIYG 78
           QIST +  +F+   AL W R K R+  LPPGPRGLPLVGYLPFL  N+H  FADLA+IYG
Sbjct: 17  QISTPLLFIFLFISALLWLRSKFRQSPLPPGPRGLPLVGYLPFLSTNLHHNFADLAKIYG 76

Query: 79  PVFKVRIGNKLCVVLNSPSSINEAFRHHETVFPNRDTTLRRLRHCLHPG--------RHD 138
           P+FK+R+G KLC VL SPSSINE  RHHETVF NRD T+   R C + G          D
Sbjct: 77  PIFKLRLGAKLCFVLTSPSSINEVLRHHETVFDNRDATV-SARLCTYGGSDIVFSQNEGD 136

Query: 139 WKKLRKIFVRKMLSRSFLDASYSVRRQEVRKVIKGVFESAGTPIDIGKVGFLATMKSVLA 198
           WKKLRKIF RKMLS++ LDASY +RRQEVRK+IKGV ESAGTPID G++ FLA +KSV+A
Sbjct: 137 WKKLRKIFARKMLSKANLDASYPLRRQEVRKIIKGVLESAGTPIDFGELSFLAAVKSVMA 196

Query: 199 MTWGDSGRLIGEDGIDLDVKFRAVMDELAVLLGTPNLSDIFPILGRFDLQGIASRTNKAM 258
           M WG SG L G    DL+ KFR V+DEL VLLGTPNLSD+FP+LG  DLQGIA +  K  
Sbjct: 197 MIWGGSGGLTGVAVTDLEAKFREVVDELMVLLGTPNLSDLFPVLGCLDLQGIARKMKKVS 256

Query: 259 RVCDEILNTAIEEQRKMGGNAV 273
             CDEILN+AIE QRK G N +
Sbjct: 257 NRCDEILNSAIEGQRKKGRNGL 277

[Close]

BLAST of Carg16694-RA vs. TrEMBL
Match: tr|A0A2P4HY33|A0A2P4HY33_QUESU (Flavonoid 3'-monooxygenase OS=Quercus suber OX=58331 GN=CFP56_60163 PE=3 SV=1)

HSP 1 Score: 246.9 bits (629), Expect = 5.6e-62
Identity = 128/285 (44.91%), Postives = 186/285 (65.26%), Query Frame = 0


Query: 2   SISAFLCSFWSLDSDEKQISTAMFLVFVVTFA-LFWF---RPKVRRPSLPPGPRGLPLVG 61
           ++SA    +WS  +D+ ++S A F   V  FA LFWF     K R P LPPGPRGLPL+G
Sbjct: 4   TVSAMWSRWWSASNDKDELSRANFTALVSIFAVLFWFLWAAKKSRNPPLPPGPRGLPLLG 63

Query: 62  YLPFLLRNVHRTFADLAEIYGPVFKVRIGNKLCVVLNSPSSINEAFRHHETVFPNRDTTL 121
           YLPFL  N+HR F +LA +YGP++KV +G KLCVV++SPS + E  R  +T+F NRD  +
Sbjct: 64  YLPFLGTNLHRKFEELAGVYGPIYKVWLGQKLCVVISSPSLVKEVVRDQDTIFANRDPPI 123

Query: 122 RRLRHCLHPG--------RHDWKKLRKIFVRKMLSRSFLDASYSVRRQEVRKVIKGVFES 181
              R   + G          +WKKLRKIFVR+MLS + LD+SY++RR+EV+K I+ V++ 
Sbjct: 124 -AARVASYGGVDIAFSSYGPEWKKLRKIFVREMLSNASLDSSYTLRREEVKKSIRNVYDK 183

Query: 182 AGTPIDIGKVGFLATMKSVLAMTWGDSGRLIGEDGIDLDVKFRAVMDELAVLLGTPNLSD 241
            GTPID+G + F+  + +++ M WGDS  L GE+G  +  +F+ ++ E  VL+  PN+SD
Sbjct: 184 IGTPIDLGVLAFVTGVNAIMNMVWGDS--LQGEEGTKIGAEFKKLVGEQMVLMAKPNVSD 243

Query: 242 IFPILGRFDLQGIASRTNKAMRVCDEILNTAIEEQ------RKMG 269
            FP+L  FDLQGI  +  K ++  + IL++AI+++      +KMG
Sbjct: 244 FFPVLAMFDLQGIERQAKKILKWVEAILDSAIDKRMNSDTGKKMG 285

The following BLAST results are available for this feature:

BLAST of Carg16694-RA vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023550541.1	8.4e-134	89.32	ferruginol synthase-like [Cucurbita pepo subsp. pepo]	[more]
XP_008437141.1	6.9e-96	64.96	PREDICTED: 7-ethoxycoumarin O-deethylase-like [Cucumis melo]	[more]
XP_008437145.1	3.2e-93	64.31	PREDICTED: 7-ethoxycoumarin O-deethylase-like [Cucumis melo]	[more]
KGN50204.1	6.1e-92	70.28	hypothetical protein Csa_5G158600 [Cucumis sativus]	[more]
XP_004147609.1	2.0e-90	70.20	PREDICTED: 7-ethoxycoumarin O-deethylase-like [Cucumis sativus]	[more]

BLAST of Carg16694-RA vs. TAIR10


Match Name	E-value	Identity	Description
AT4G12300.1	8.0e-46	38.58	cytochrome P450, family 706, subfamily A, polypeptide 4	[more]
AT4G12310.1	1.8e-45	37.70	cytochrome P450, family 706, subfamily A, polypeptide 5	[more]
AT4G12320.1	5.7e-44	37.60	cytochrome P450, family 706, subfamily A, polypeptide 6	[more]
AT4G12330.1	3.0e-37	33.33	cytochrome P450, family 706, subfamily A, polypeptide 7	[more]
AT5G44620.1	2.6e-36	32.45	cytochrome P450, family 706, subfamily A, polypeptide 3	[more]

BLAST of Carg16694-RA vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|S4UX02\|CYPH1_SALMI	1.0e-26	28.85	Ferruginol synthase OS=Salvia miltiorrhiza OX=226208 GN=CYP76AH1 PE=1 SV=1	[more]
sp\|H2DH18\|C7A12_PANGI	3.7e-24	27.56	Cytochrome P450 CYP736A12 OS=Panax ginseng OX=4054 PE=2 SV=1	[more]
sp\|Q42600\|C84A1_ARATH	1.4e-23	30.35	Cytochrome P450 84A1 OS=Arabidopsis thaliana OX=3702 GN=CYP84A1 PE=1 SV=1	[more]
sp\|Q6WNQ9\|C81E9_MEDTR	4.1e-23	30.42	Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 P...	[more]
sp\|F4JW83\|C84A4_ARATH	9.1e-23	28.91	Cytochrome P450 84A4 OS=Arabidopsis thaliana OX=3702 GN=CYP84A4 PE=1 SV=1	[more]

BLAST of Carg16694-RA vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A1S3ATA1\|A0A1S3ATA1_CUCME	4.6e-96	64.96	7-ethoxycoumarin O-deethylase-like OS=Cucumis melo OX=3656 GN=LOC103482652 PE=3 ...	[more]
tr\|A0A1S3ATF6\|A0A1S3ATF6_CUCME	2.1e-93	64.31	7-ethoxycoumarin O-deethylase-like OS=Cucumis melo OX=3656 GN=LOC103482655 PE=3 ...	[more]
tr\|A0A0A0KMT1\|A0A0A0KMT1_CUCSA	4.0e-92	70.28	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G158600 PE=4 SV=1	[more]
tr\|A0A1S3ATW0\|A0A1S3ATW0_CUCME	1.9e-86	63.36	flavonoid 3'-monooxygenase-like OS=Cucumis melo OX=3656 GN=LOC103482653 PE=3 SV=...	[more]
tr\|A0A2P4HY33\|A0A2P4HY33_QUESU	5.6e-62	44.91	Flavonoid 3'-monooxygenase OS=Quercus suber OX=58331 GN=CFP56_60163 PE=3 SV=1	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **Biological Process**
Term	Definition
GO:0055114	oxidation-reduction process

Vocabulary: **Molecular Function**
Term	Definition
GO:0005506	iron ion binding
GO:0020037	heme binding
GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen

Vocabulary: **INTERPRO**
Term	Definition
IPR036396	Cyt_P450_sf
IPR001128	Cyt_P450

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055114	oxidation-reduction process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Carg16694	Carg16694	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Carg16694-RA	Carg16694-RA-protein	polypeptide

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg16694-RA.CDS.3	Carg16694-RA.CDS.3	CDS
Carg16694-RA.CDS.2	Carg16694-RA.CDS.2	CDS
Carg16694-RA.CDS.1	Carg16694-RA.CDS.1	CDS

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg16694-RA.exon.3	Carg16694-RA.exon.3	exon
Carg16694-RA.exon.2	Carg16694-RA.exon.2	exon
Carg16694-RA.exon.1	Carg16694-RA.exon.1	exon

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 47..266 e-value: 2.0E-20 score: 72.9
IPR036396	Cytochrome P450 superfamily	GENE3D	G3DSA:1.10.630.10		coord: 40..278 e-value: 8.2E-35 score: 122.4
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	SSF48264	Cytochrome P450	coord: 41..267
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 268..294
None	No IPR available	PANTHER	PTHR24298	FAMILY NOT NAMED	coord: 22..274
None	No IPR available	PANTHER	PTHR24298:SF312	SUBFAMILY NOT NAMED	coord: 22..274