Carg15615-RA (mRNA) Silver-seed gourd

NameCarg15615-RA
TypemRNA
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionGalactinol--sucrose galactosyltransferase
LocationCucurbita_argyrosperma_scaffold_059 : 547819 .. 550522 (-)
Sequence length2190
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTCCTATTTTGAAAAATGGCAATTCCAACATTGTCGTTTCATTCGATGGCTTGAACGACTTGTCGTCTCCATTCTCGATCGACGGGTCGAATTTCACTGTTAACGGCCATACCTTCTTGTCGGATGTGCCTGAGAATATTGTCGCTTCTCCGTCTCCGTACACTACGATCGATAAGTCCCCGGTTTCAATTGGTTGCTTTGTAGGATTCGACGTGTTGGACCCTGATAGTCGACATGTCGTTTCGATTGGTAAGCTGAAGGATATTAAGTTTATGAGTATTTTCAGGTTTAAGGTATGGTGGACTACTCATTGGGTTGGTCGGAATGGTGGTGACCTTGAGTCGGAGACTCAAATTGTGATCCTTGAGAAGTCGGATTCTGGTCGGCCGTATGTTCTCCTTCTTCCCCTCGTTGAGGGGCCGTTCCGATCTTCGATTCAGCCTGGGGAGGATGACTTTGTCGACGTTTGTGTCGAGAGTGGCTCGTCCAAAGTGGTGGACTCGTCGTTTCGAAGCGTGTTGTATCTTCATGCTGGTGATGATCCGTTTGTACTTGTTAAGGAGGCGATGAAGATCGTTAGGACCCATCTCGGAACTTTCCGCTTGTTGGAGGAGAAGACTCCACCAGGTATATATTCTTAACCTAAACTCAAAACGTTTTGTGTTAGATAATCCATGATCTTCTTCTTAATTAGTCAATGTATATATTATCACTAGGTATCGTGGACAAATTCGGGTGGTGCACGTGGGATGCTTTCTACCTAACGGTTCACCCACAAGGCATAATGGAAGGTGTGAAGCATCTCGTCGATGGCGGTTGCCCTCCCGGCCTAGTCCTTATCGATGATGGTTGGCAGTCGATCGGCCACGATGCTGATCCCATCACCAAAGAAGGAATGAACCAAACCGTTGCTGGCGAGCAAATGCCCTGTCGCCTTCTAAAATTCCAAGAGAATTACAAATTCCGTGACTATGTCAACCCGAAGAGCTCCGGTGTTGGCTCCGGCCAGAAGGGGATGAAGGCGTTCATTGATGAACTCAAAGGGGAGTACAAGACGGTAGAGTATGTGTATGTGTGGCATGCTTTGTGTGGATACTGGGGTGGGCTCCGGCCGAACGTGCCAGGGTTACCGGAGGCTAACGTGATCCAACCGGTCCTCTCGCCGGGCCTGCAACAGACGATGGAAGATTTGGCCGTCGATAAGATTGTTTGTCATAAGGTCGGGATAGTCCCACCAGAGAAGGCTGAGGAGATGTACGAGGGACTCCATGCTCATTTGGAGAATGTCGGGATTGATGGTGTTAAGATCGACGTCATTCATGTAAGCACCGTTAGTAAATCTTATTTTGATATTTGAACGTTTTGCTATGTTATTGAGCCCGTGTCATAGATAATATTATCGTACTATCGTAAATTTGAATGGACAGTTGTTGGAGATGCTATGCGAGGACTATGGTGGTCGAGTGGATTTGGCAAAGGCATATTACAAAGCCATGTCCAAATCAATAAAGAAACATTTCAAAGGAAACGGAGTGATCGCGAGTATGGAACATTGTAACGACTTCATGTTCCTCGGCACGGAAGCTATTTCTCTCGGTCGTGTTGGTAAATAATTTTTCTTTTTTAAATTTATTTGGTGATGACGGGTTACTCTCTACATCCAATTAAAGAGTGCCACGTGTTGTTTAATAGGTGATGACTTTTGGTGCACCGACCCTTCTGGTGATCCGAATGGTACGTTTTGGCTTCAAGGATGCCACATGGTGCACTGTGCCTACAACAGTCTGTGGATGGGCAACTTCATCCACCCCGACTGGGATATGTTCCAATCTACCCACCCTTGTGCTGCCTTCCATGCTGCCTCTCGAGCTATCTCTGGTGGCCCGATCTATGTCAGTGACTTTGTCGGGAAACACAACTTTGAGCTCCTTAGAAAATTGGTTCTCCCTGATGGCTCGATCCTTCGTAGTGAGTACTATGCTCTCCCGACTCGTGATTGTCTCTTCGATGATCCCTTGCACGACGGAGAAACTATGCTCAAGATATGGAACCTTAACAAGGTAAACGTTTTTTTCTATATTGGATCATTGTGTTGAATGTTTGTAAAGTATTTTAAGATATTATTTTTTTGCACAGTTCACTGGTGTGATCGGTGCATTCAACTGTCAAGGAGGAGGGTGGTGTCGTGAGACGCGACGTAATCAGTGCTTCTCTCAATACTCGAAGCGAGTGACCTCAAAAACTAATGCAAAAGACATCGAATGGAATAGCGGAGAAAATCCAATCTCTATCGAGGGTGTGAAAACTTTTGCGCTGTACCTCTACCAAGCTAAAAAGCTGATCCTTGCCAAGCCCACCCAAAACATTGATATTGCCCTCGATCCCTTCGACTTTGAGCTTATCTTTGTCTCTCCCGTCACCACACTCATCGAAACGGCTGTCCAGTTCGCCCCAATTGGGCTCGTTAATATGCTGAACATTGGTGGTGCCATCCAGTCTGTGTACTATGATGATGACCTAAGCTCGGTCGAAATTGGCGTCAAAGGTACTGGTGAGATGCGAGTGTATGCGTCTGACAAACCTAGGGCTTGTCGGATTGACGGTGAGGATGTTGGGTTCAAGTATCAAGACCAAATGGTGGCGGTCCAAGTGCCATGGCCCGGTTCTTCTGGCATTTCAACTATCGAGTACTTATTTTAA

mRNA sequence

ATGGCTCCTATTTTGAAAAATGGCAATTCCAACATTGTCGTTTCATTCGATGGCTTGAACGACTTGTCGTCTCCATTCTCGATCGACGGGTCGAATTTCACTGTTAACGGCCATACCTTCTTGTCGGATGTGCCTGAGAATATTGTCGCTTCTCCGTCTCCGTACACTACGATCGATAAGTCCCCGGTTTCAATTGGTTGCTTTGTAGGATTCGACGTGTTGGACCCTGATAGTCGACATGTCGTTTCGATTGGTAAGCTGAAGGATATTAAGTTTATGAGTATTTTCAGGTTTAAGGTATGGTGGACTACTCATTGGGTTGGTCGGAATGGTGGTGACCTTGAGTCGGAGACTCAAATTGTGATCCTTGAGAAGTCGGATTCTGGTCGGCCGTATGTTCTCCTTCTTCCCCTCGTTGAGGGGCCGTTCCGATCTTCGATTCAGCCTGGGGAGGATGACTTTGTCGACGTTTGTGTCGAGAGTGGCTCGTCCAAAGTGGTGGACTCGTCGTTTCGAAGCGTGTTGTATCTTCATGCTGGCATAATGGAAGGTGTGAAGCATCTCGTCGATGGCGGTTGCCCTCCCGGCCTAGTCCTTATCGATGATGGTTGGCAGTCGATCGGCCACGATGCTGATCCCATCACCAAAGAAGGAATGAACCAAACCGTTGCTGGCGAGCAAATGCCCTGTCGCCTTCTAAAATTCCAAGAGAATTACAAATTCCGTGACTATGTCAACCCGAAGAGCTCCGGTGTTGGCTCCGGCCAGAAGGGGATGAAGGCGTTCATTGATGAACTCAAAGGGGAGTACAAGACGGTAGAGTATGTGTATGTGTGGCATGCTTTGTGTGGATACTGGGGTGGGCTCCGGCCGAACGTGCCAGGGTTACCGGAGGCTAACGTGATCCAACCGGTCCTCTCGCCGGGCCTGCAACAGACGATGGAAGATTTGGCCGTCGATAAGATTGTTTGTCATAAGGTCGGGATAGTCCCACCAGAGAAGGCTGAGGAGATGTACGAGGGACTCCATGCTCATTTGGAGAATGTCGGGATTGATGGTGTTAAGATCGACGTCATTCATTTGTTGGAGATGCTATGCGAGGACTATGGTGGTCGAGTGGATTTGGCAAAGGCATATTACAAAGCCATGTCCAAATCAATAAAGAAACATTTCAAAGGAAACGGAGTGATCGCGAGTATGGAACATTGTAACGACTTCATGTTCCTCGGCACGGAAGCTATTTCTCTCGGTCGTGTTGGTGATGACTTTTGGTGCACCGACCCTTCTGGTGATCCGAATGGTACGTTTTGGCTTCAAGGATGCCACATGGTGCACTGTGCCTACAACAGTCTGTGGATGGGCAACTTCATCCACCCCGACTGGGATATGTTCCAATCTACCCACCCTTGTGCTGCCTTCCATGCTGCCTCTCGAGCTATCTCTGGTGGCCCGATCTATGTCAGTGACTTTGTCGGGAAACACAACTTTGAGCTCCTTAGAAAATTGGTTCTCCCTGATGGCTCGATCCTTCGTAGTGAGTACTATGCTCTCCCGACTCGTGATTGTCTCTTCGATGATCCCTTGCACGACGGAGAAACTATGCTCAAGATATGGAACCTTAACAAGTTCACTGGTGTGATCGGTGCATTCAACTGTCAAGGAGGAGGGTGGTGTCGTGAGACGCGACGTAATCAGTGCTTCTCTCAATACTCGAAGCGAGTGACCTCAAAAACTAATGCAAAAGACATCGAATGGAATAGCGGAGAAAATCCAATCTCTATCGAGGGTGTGAAAACTTTTGCGCTGTACCTCTACCAAGCTAAAAAGCTGATCCTTGCCAAGCCCACCCAAAACATTGATATTGCCCTCGATCCCTTCGACTTTGAGCTTATCTTTGTCTCTCCCGTCACCACACTCATCGAAACGGCTGTCCAGTTCGCCCCAATTGGGCTCGTTAATATGCTGAACATTGGTGGTGCCATCCAGTCTGTGTACTATGATGATGACCTAAGCTCGGTCGAAATTGGCGTCAAAGGTACTGGTGAGATGCGAGTGTATGCGTCTGACAAACCTAGGGCTTGTCGGATTGACGGTGAGGATGTTGGGTTCAAGTATCAAGACCAAATGGTGGCGGTCCAAGTGCCATGGCCCGGTTCTTCTGGCATTTCAACTATCGAGTACTTATTTTAA

Coding sequence (CDS)

ATGGCTCCTATTTTGAAAAATGGCAATTCCAACATTGTCGTTTCATTCGATGGCTTGAACGACTTGTCGTCTCCATTCTCGATCGACGGGTCGAATTTCACTGTTAACGGCCATACCTTCTTGTCGGATGTGCCTGAGAATATTGTCGCTTCTCCGTCTCCGTACACTACGATCGATAAGTCCCCGGTTTCAATTGGTTGCTTTGTAGGATTCGACGTGTTGGACCCTGATAGTCGACATGTCGTTTCGATTGGTAAGCTGAAGGATATTAAGTTTATGAGTATTTTCAGGTTTAAGGTATGGTGGACTACTCATTGGGTTGGTCGGAATGGTGGTGACCTTGAGTCGGAGACTCAAATTGTGATCCTTGAGAAGTCGGATTCTGGTCGGCCGTATGTTCTCCTTCTTCCCCTCGTTGAGGGGCCGTTCCGATCTTCGATTCAGCCTGGGGAGGATGACTTTGTCGACGTTTGTGTCGAGAGTGGCTCGTCCAAAGTGGTGGACTCGTCGTTTCGAAGCGTGTTGTATCTTCATGCTGGCATAATGGAAGGTGTGAAGCATCTCGTCGATGGCGGTTGCCCTCCCGGCCTAGTCCTTATCGATGATGGTTGGCAGTCGATCGGCCACGATGCTGATCCCATCACCAAAGAAGGAATGAACCAAACCGTTGCTGGCGAGCAAATGCCCTGTCGCCTTCTAAAATTCCAAGAGAATTACAAATTCCGTGACTATGTCAACCCGAAGAGCTCCGGTGTTGGCTCCGGCCAGAAGGGGATGAAGGCGTTCATTGATGAACTCAAAGGGGAGTACAAGACGGTAGAGTATGTGTATGTGTGGCATGCTTTGTGTGGATACTGGGGTGGGCTCCGGCCGAACGTGCCAGGGTTACCGGAGGCTAACGTGATCCAACCGGTCCTCTCGCCGGGCCTGCAACAGACGATGGAAGATTTGGCCGTCGATAAGATTGTTTGTCATAAGGTCGGGATAGTCCCACCAGAGAAGGCTGAGGAGATGTACGAGGGACTCCATGCTCATTTGGAGAATGTCGGGATTGATGGTGTTAAGATCGACGTCATTCATTTGTTGGAGATGCTATGCGAGGACTATGGTGGTCGAGTGGATTTGGCAAAGGCATATTACAAAGCCATGTCCAAATCAATAAAGAAACATTTCAAAGGAAACGGAGTGATCGCGAGTATGGAACATTGTAACGACTTCATGTTCCTCGGCACGGAAGCTATTTCTCTCGGTCGTGTTGGTGATGACTTTTGGTGCACCGACCCTTCTGGTGATCCGAATGGTACGTTTTGGCTTCAAGGATGCCACATGGTGCACTGTGCCTACAACAGTCTGTGGATGGGCAACTTCATCCACCCCGACTGGGATATGTTCCAATCTACCCACCCTTGTGCTGCCTTCCATGCTGCCTCTCGAGCTATCTCTGGTGGCCCGATCTATGTCAGTGACTTTGTCGGGAAACACAACTTTGAGCTCCTTAGAAAATTGGTTCTCCCTGATGGCTCGATCCTTCGTAGTGAGTACTATGCTCTCCCGACTCGTGATTGTCTCTTCGATGATCCCTTGCACGACGGAGAAACTATGCTCAAGATATGGAACCTTAACAAGTTCACTGGTGTGATCGGTGCATTCAACTGTCAAGGAGGAGGGTGGTGTCGTGAGACGCGACGTAATCAGTGCTTCTCTCAATACTCGAAGCGAGTGACCTCAAAAACTAATGCAAAAGACATCGAATGGAATAGCGGAGAAAATCCAATCTCTATCGAGGGTGTGAAAACTTTTGCGCTGTACCTCTACCAAGCTAAAAAGCTGATCCTTGCCAAGCCCACCCAAAACATTGATATTGCCCTCGATCCCTTCGACTTTGAGCTTATCTTTGTCTCTCCCGTCACCACACTCATCGAAACGGCTGTCCAGTTCGCCCCAATTGGGCTCGTTAATATGCTGAACATTGGTGGTGCCATCCAGTCTGTGTACTATGATGATGACCTAAGCTCGGTCGAAATTGGCGTCAAAGGTACTGGTGAGATGCGAGTGTATGCGTCTGACAAACCTAGGGCTTGTCGGATTGACGGTGAGGATGTTGGGTTCAAGTATCAAGACCAAATGGTGGCGGTCCAAGTGCCATGGCCCGGTTCTTCTGGCATTTCAACTATCGAGTACTTATTTTAA

Protein sequence

MAPILKNGNSNIVVSFDGLNDLSSPFSIDGSNFTVNGHTFLSDVPENIVASPSPYTTIDKSPVSIGCFVGFDVLDPDSRHVVSIGKLKDIKFMSIFRFKVWWTTHWVGRNGGDLESETQIVILEKSDSGRPYVLLLPLVEGPFRSSIQPGEDDFVDVCVESGSSKVVDSSFRSVLYLHAGIMEGVKHLVDGGCPPGLVLIDDGWQSIGHDADPITKEGMNQTVAGEQMPCRLLKFQENYKFRDYVNPKSSGVGSGQKGMKAFIDELKGEYKTVEYVYVWHALCGYWGGLRPNVPGLPEANVIQPVLSPGLQQTMEDLAVDKIVCHKVGIVPPEKAEEMYEGLHAHLENVGIDGVKIDVIHLLEMLCEDYGGRVDLAKAYYKAMSKSIKKHFKGNGVIASMEHCNDFMFLGTEAISLGRVGDDFWCTDPSGDPNGTFWLQGCHMVHCAYNSLWMGNFIHPDWDMFQSTHPCAAFHAASRAISGGPIYVSDFVGKHNFELLRKLVLPDGSILRSEYYALPTRDCLFDDPLHDGETMLKIWNLNKFTGVIGAFNCQGGGWCRETRRNQCFSQYSKRVTSKTNAKDIEWNSGENPISIEGVKTFALYLYQAKKLILAKPTQNIDIALDPFDFELIFVSPVTTLIETAVQFAPIGLVNMLNIGGAIQSVYYDDDLSSVEIGVKGTGEMRVYASDKPRACRIDGEDVGFKYQDQMVAVQVPWPGSSGISTIEYLF
BLAST of Carg15615-RA vs. NCBI nr
Match: XP_022928549.1 (probable galactinol--sucrose galactosyltransferase 5 [Cucurbita moschata])

HSP 1 Score: 1504.6 bits (3894), Expect = 0.0e+00
Identity = 728/781 (93.21%), Postives = 728/781 (93.21%), Query Frame = 0

Query: 1   MAPILKNGNSNIVVSFDGLNDLSSPFSIDGSNFTVNGHTFLSDVPENIVASPSPYTTIDK 60
           MAPILKNGNSNIVVSFDGLNDLSSPFSIDGSNFTVNGHTFLSDVPENIVASPSPYTTIDK
Sbjct: 1   MAPILKNGNSNIVVSFDGLNDLSSPFSIDGSNFTVNGHTFLSDVPENIVASPSPYTTIDK 60

Query: 61  SPVSIGCFVGFDVLDPDSRHVVSIGKLKDIKFMSIFRFKVWWTTHWVGRNGGDLESETQI 120
           SPVSIGCFVGFDVLDPDSRHVVSIGKLKDIKFMSIFRFKVWWTTHWVGRNGGDLESETQI
Sbjct: 61  SPVSIGCFVGFDVLDPDSRHVVSIGKLKDIKFMSIFRFKVWWTTHWVGRNGGDLESETQI 120

Query: 121 VILEKSDSGRPYVLLLPLVEGPFRSSIQPGEDDFVDVCVESGSSKVVDSSFRSVLYLHA- 180
           VILEKSDSGRPYVLLLPLVEGPFRSSIQPGEDDFVDVCVESGSSKVVDSSFRSVLYLHA 
Sbjct: 121 VILEKSDSGRPYVLLLPLVEGPFRSSIQPGEDDFVDVCVESGSSKVVDSSFRSVLYLHAG 180

Query: 181 ---------------------------------------------------GIMEGVKHL 240
                                                              GIMEGVKHL
Sbjct: 181 DDPFVLVKEAMKIVRTHLGTFRLLEEKTPPGIVDKFGWCTWDAFYLTVHPQGIMEGVKHL 240

Query: 241 VDGGCPPGLVLIDDGWQSIGHDADPITKEGMNQTVAGEQMPCRLLKFQENYKFRDYVNPK 300
           VDGGCPPGLVLIDDGWQSIGHDADPITKEGMNQTVAGEQMPCRLLKFQENYKFRDYVNPK
Sbjct: 241 VDGGCPPGLVLIDDGWQSIGHDADPITKEGMNQTVAGEQMPCRLLKFQENYKFRDYVNPK 300

Query: 301 SSGVGSGQKGMKAFIDELKGEYKTVEYVYVWHALCGYWGGLRPNVPGLPEANVIQPVLSP 360
           SSGVGSGQKGMKAFIDELKGEYKTVEYVYVWHALCGYWGGLRPNVPGLPEANVIQPVLSP
Sbjct: 301 SSGVGSGQKGMKAFIDELKGEYKTVEYVYVWHALCGYWGGLRPNVPGLPEANVIQPVLSP 360

Query: 361 GLQQTMEDLAVDKIVCHKVGIVPPEKAEEMYEGLHAHLENVGIDGVKIDVIHLLEMLCED 420
           GLQQTMEDLAVDKIVCHKVGIVPPEKAEEMYEGLHAHLENVGIDGVKIDVIHLLEMLCED
Sbjct: 361 GLQQTMEDLAVDKIVCHKVGIVPPEKAEEMYEGLHAHLENVGIDGVKIDVIHLLEMLCED 420

Query: 421 YGGRVDLAKAYYKAMSKSIKKHFKGNGVIASMEHCNDFMFLGTEAISLGRVGDDFWCTDP 480
           YGGRVDLAKAYYKAMSKSIKKHFKGNGVIASMEHCNDFMFLGTEAISLGRVGDDFWCTDP
Sbjct: 421 YGGRVDLAKAYYKAMSKSIKKHFKGNGVIASMEHCNDFMFLGTEAISLGRVGDDFWCTDP 480

Query: 481 SGDPNGTFWLQGCHMVHCAYNSLWMGNFIHPDWDMFQSTHPCAAFHAASRAISGGPIYVS 540
           SGDPNGTFWLQGCHMVHCAYNSLWMGNFIHPDWDMFQSTHPCAAFHAASRAISGGPIYVS
Sbjct: 481 SGDPNGTFWLQGCHMVHCAYNSLWMGNFIHPDWDMFQSTHPCAAFHAASRAISGGPIYVS 540

Query: 541 DFVGKHNFELLRKLVLPDGSILRSEYYALPTRDCLFDDPLHDGETMLKIWNLNKFTGVIG 600
           DFVGKHNFELLRKLVLPDGSILRSEYYALPTRDCLFDDPLHDGETMLKIWNLNKFTGVIG
Sbjct: 541 DFVGKHNFELLRKLVLPDGSILRSEYYALPTRDCLFDDPLHDGETMLKIWNLNKFTGVIG 600

Query: 601 AFNCQGGGWCRETRRNQCFSQYSKRVTSKTNAKDIEWNSGENPISIEGVKTFALYLYQAK 660
           AFNCQGGGWCRETRRNQCFSQYSKRVTSKTNAKDIEWNSGENPISIEGVKTFALYLYQAK
Sbjct: 601 AFNCQGGGWCRETRRNQCFSQYSKRVTSKTNAKDIEWNSGENPISIEGVKTFALYLYQAK 660

Query: 661 KLILAKPTQNIDIALDPFDFELIFVSPVTTLIETAVQFAPIGLVNMLNIGGAIQSVYYDD 720
           KLILAKPTQNIDIALDPFDFELIFVSPV TLIETAVQFAPIGLVNMLNIGGAIQSVYYDD
Sbjct: 661 KLILAKPTQNIDIALDPFDFELIFVSPVNTLIETAVQFAPIGLVNMLNIGGAIQSVYYDD 720

Query: 721 DLSSVEIGVKGTGEMRVYASDKPRACRIDGEDVGFKYQDQMVAVQVPWPGSSGISTIEYL 730
           DLSSVEIGVKGTGEMRVYASDKPRACRIDGEDVGFKYQDQMVAVQVPWPGSSGISTIEYL
Sbjct: 721 DLSSVEIGVKGTGEMRVYASDKPRACRIDGEDVGFKYQDQMVAVQVPWPGSSGISTIEYL 780

BLAST of Carg15615-RA vs. NCBI nr
Match: XP_022989256.1 (probable galactinol--sucrose galactosyltransferase 5 [Cucurbita maxima])

HSP 1 Score: 1489.6 bits (3855), Expect = 0.0e+00
Identity = 718/781 (91.93%), Postives = 725/781 (92.83%), Query Frame = 0

Query: 1   MAPILKNGNSNIVVSFDGLNDLSSPFSIDGSNFTVNGHTFLSDVPENIVASPSPYTTIDK 60
           MAPILKNGNSN VVSFDGLNDLSSPFSI+GSNFTVNGHTFLSDVPENIVASPSPYTTIDK
Sbjct: 1   MAPILKNGNSNFVVSFDGLNDLSSPFSIEGSNFTVNGHTFLSDVPENIVASPSPYTTIDK 60

Query: 61  SPVSIGCFVGFDVLDPDSRHVVSIGKLKDIKFMSIFRFKVWWTTHWVGRNGGDLESETQI 120
           SPVSIGCFVGFDVLDPDSRHVVSIGKLKDIKFMSIFRFKVWWTTHWVGRNGGDLESETQI
Sbjct: 61  SPVSIGCFVGFDVLDPDSRHVVSIGKLKDIKFMSIFRFKVWWTTHWVGRNGGDLESETQI 120

Query: 121 VILEKSDSGRPYVLLLPLVEGPFRSSIQPGEDDFVDVCVESGSSKVVDSSFRSVLYLHA- 180
           VILEKSDSGRPYVLLLPLVEGPFRSSIQPGEDDFVDVCVESGSSKVVDSSFRSVLYLHA 
Sbjct: 121 VILEKSDSGRPYVLLLPLVEGPFRSSIQPGEDDFVDVCVESGSSKVVDSSFRSVLYLHAG 180

Query: 181 ---------------------------------------------------GIMEGVKHL 240
                                                              GIMEGVKHL
Sbjct: 181 DDPFVLVKEAMKIVRTHLGTFRLLEEKTPPGIVDKFGWCTWDAFYLTVHPQGIMEGVKHL 240

Query: 241 VDGGCPPGLVLIDDGWQSIGHDADPITKEGMNQTVAGEQMPCRLLKFQENYKFRDYVNPK 300
           VDGGCPPGLVLIDDGWQSIGHDADPITKEGMNQTVAGEQMPCRLLKFQENYKFRDYVNPK
Sbjct: 241 VDGGCPPGLVLIDDGWQSIGHDADPITKEGMNQTVAGEQMPCRLLKFQENYKFRDYVNPK 300

Query: 301 SSGVGSGQKGMKAFIDELKGEYKTVEYVYVWHALCGYWGGLRPNVPGLPEANVIQPVLSP 360
           SS   SGQKGMKAFIDELKGEYKTVEYVYVWHALCGYWGGLRP+VPGLPEANVIQPVLSP
Sbjct: 301 SSSAVSGQKGMKAFIDELKGEYKTVEYVYVWHALCGYWGGLRPDVPGLPEANVIQPVLSP 360

Query: 361 GLQQTMEDLAVDKIVCHKVGIVPPEKAEEMYEGLHAHLENVGIDGVKIDVIHLLEMLCED 420
           GLQQTMEDLAVDKIVCHKVG+VPPEKAEEMYEGLHAHLENVGIDGVKIDVIHLLEMLCED
Sbjct: 361 GLQQTMEDLAVDKIVCHKVGMVPPEKAEEMYEGLHAHLENVGIDGVKIDVIHLLEMLCED 420

Query: 421 YGGRVDLAKAYYKAMSKSIKKHFKGNGVIASMEHCNDFMFLGTEAISLGRVGDDFWCTDP 480
           YGGRVDLAKAYYKAMSKSIKKHFKGNGVIASMEHCNDFMFLGTEAISLGRVGDDFWCTDP
Sbjct: 421 YGGRVDLAKAYYKAMSKSIKKHFKGNGVIASMEHCNDFMFLGTEAISLGRVGDDFWCTDP 480

Query: 481 SGDPNGTFWLQGCHMVHCAYNSLWMGNFIHPDWDMFQSTHPCAAFHAASRAISGGPIYVS 540
           SGDPNGTFWLQGCHMVHCA+NSLWMGNFIHPDWDMFQSTHPCAAFHAASRAISGGPIYVS
Sbjct: 481 SGDPNGTFWLQGCHMVHCAFNSLWMGNFIHPDWDMFQSTHPCAAFHAASRAISGGPIYVS 540

Query: 541 DFVGKHNFELLRKLVLPDGSILRSEYYALPTRDCLFDDPLHDGETMLKIWNLNKFTGVIG 600
           DFVGKHNFELL+KLVLPDGSILRSEYYALPTRDCLFDDPLHDGETMLKIWNLNKFTGVIG
Sbjct: 541 DFVGKHNFELLKKLVLPDGSILRSEYYALPTRDCLFDDPLHDGETMLKIWNLNKFTGVIG 600

Query: 601 AFNCQGGGWCRETRRNQCFSQYSKRVTSKTNAKDIEWNSGENPISIEGVKTFALYLYQAK 660
           AFNCQGGGWCRETRRNQCFSQYSKRVTSKTNAKDIEWNSGENPISIEGVKTFALYLYQAK
Sbjct: 601 AFNCQGGGWCRETRRNQCFSQYSKRVTSKTNAKDIEWNSGENPISIEGVKTFALYLYQAK 660

Query: 661 KLILAKPTQNIDIALDPFDFELIFVSPVTTLIETAVQFAPIGLVNMLNIGGAIQSVYYDD 720
           KLILAKPTQNIDIALDPFDFELIFVSPVTTL+ETAVQFAPIGLVNMLNIGGAIQSVYYDD
Sbjct: 661 KLILAKPTQNIDIALDPFDFELIFVSPVTTLVETAVQFAPIGLVNMLNIGGAIQSVYYDD 720

Query: 721 DLSSVEIGVKGTGEMRVYASDKPRACRIDGEDVGFKYQDQMVAVQVPWPGSSGISTIEYL 730
           DLSSVEIG+KGTGEMRVYASDKPRACRIDGEDVGFKYQDQMVAVQVPWPGSSGISTIEYL
Sbjct: 721 DLSSVEIGIKGTGEMRVYASDKPRACRIDGEDVGFKYQDQMVAVQVPWPGSSGISTIEYL 780

BLAST of Carg15615-RA vs. NCBI nr
Match: XP_023529233.1 (probable galactinol--sucrose galactosyltransferase 5 [Cucurbita pepo subsp. pepo] >XP_023529273.1 probable galactinol--sucrose galactosyltransferase 5 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1486.9 bits (3848), Expect = 0.0e+00
Identity = 717/781 (91.81%), Postives = 726/781 (92.96%), Query Frame = 0

Query: 1   MAPILKNGNSNIVVSFDGLNDLSSPFSIDGSNFTVNGHTFLSDVPENIVASPSPYTTIDK 60
           MAPILKNGNSNIVVSFDGLNDLSSPFSIDGSNFTVNGHTFLS+VPENIVASPSPYTTIDK
Sbjct: 1   MAPILKNGNSNIVVSFDGLNDLSSPFSIDGSNFTVNGHTFLSNVPENIVASPSPYTTIDK 60

Query: 61  SPVSIGCFVGFDVLDPDSRHVVSIGKLKDIKFMSIFRFKVWWTTHWVGRNGGDLESETQI 120
           SPVSIGCFVGFDVLDP+SRHVVSIGKLKDIKFMSIFRFKVWWTTHWVGRNGGDLESETQI
Sbjct: 61  SPVSIGCFVGFDVLDPNSRHVVSIGKLKDIKFMSIFRFKVWWTTHWVGRNGGDLESETQI 120

Query: 121 VILEKSDSGRPYVLLLPLVEGPFRSSIQPGEDDFVDVCVESGSSKVVDSSFRSVLYLHA- 180
           VILEKSDSGRPYVLLLPLVEG FRSSIQPGEDDFVDVCVESGSSKVVDSSFRSVLYLHA 
Sbjct: 121 VILEKSDSGRPYVLLLPLVEGSFRSSIQPGEDDFVDVCVESGSSKVVDSSFRSVLYLHAG 180

Query: 181 ---------------------------------------------------GIMEGVKHL 240
                                                              GIMEGVKHL
Sbjct: 181 DDPFVLVKEAMKIVRTHLGTFRLLEEKTPPGIVDKFGWCTWDAFYLTVHPQGIMEGVKHL 240

Query: 241 VDGGCPPGLVLIDDGWQSIGHDADPITKEGMNQTVAGEQMPCRLLKFQENYKFRDYVNPK 300
           VDGGCPPGLVLIDDGWQSIGHDADPITKEGMNQTVAGEQMPCRLLKFQENYKFRDYVNPK
Sbjct: 241 VDGGCPPGLVLIDDGWQSIGHDADPITKEGMNQTVAGEQMPCRLLKFQENYKFRDYVNPK 300

Query: 301 SSGVGSGQKGMKAFIDELKGEYKTVEYVYVWHALCGYWGGLRPNVPGLPEANVIQPVLSP 360
           SS  GSGQKGMKAFIDELKGEYKTVEYVYVWHALCGYWGGLRP+VPGLPEANVIQP+LSP
Sbjct: 301 SSDAGSGQKGMKAFIDELKGEYKTVEYVYVWHALCGYWGGLRPDVPGLPEANVIQPILSP 360

Query: 361 GLQQTMEDLAVDKIVCHKVGIVPPEKAEEMYEGLHAHLENVGIDGVKIDVIHLLEMLCED 420
           GLQQTMEDLAVDKIVCHKVG+VPPEKAEEMYEGLHAHLENVGIDGVKIDVIHLLEMLCED
Sbjct: 361 GLQQTMEDLAVDKIVCHKVGMVPPEKAEEMYEGLHAHLENVGIDGVKIDVIHLLEMLCED 420

Query: 421 YGGRVDLAKAYYKAMSKSIKKHFKGNGVIASMEHCNDFMFLGTEAISLGRVGDDFWCTDP 480
           +GGRVDLAKAYYKAMSKSIKKHFKGNGVIASMEHCNDFMFLGTEAISLGRVGDDFWCTDP
Sbjct: 421 HGGRVDLAKAYYKAMSKSIKKHFKGNGVIASMEHCNDFMFLGTEAISLGRVGDDFWCTDP 480

Query: 481 SGDPNGTFWLQGCHMVHCAYNSLWMGNFIHPDWDMFQSTHPCAAFHAASRAISGGPIYVS 540
           SGDPNGTFWLQGCHMVHCA+NSLWMGNFIHPDWDMFQSTHPCAAFHAASRAISGGPIYVS
Sbjct: 481 SGDPNGTFWLQGCHMVHCAFNSLWMGNFIHPDWDMFQSTHPCAAFHAASRAISGGPIYVS 540

Query: 541 DFVGKHNFELLRKLVLPDGSILRSEYYALPTRDCLFDDPLHDGETMLKIWNLNKFTGVIG 600
           DFVGKHNFELLRKLVLPDGSILRSEYYALPTRDCLFDDPLHDGETMLKIWNLNKFTGVIG
Sbjct: 541 DFVGKHNFELLRKLVLPDGSILRSEYYALPTRDCLFDDPLHDGETMLKIWNLNKFTGVIG 600

Query: 601 AFNCQGGGWCRETRRNQCFSQYSKRVTSKTNAKDIEWNSGENPISIEGVKTFALYLYQAK 660
           AFNCQGGGWCRETRRNQCFSQYSKRVTSKTNAKDIEWNSGENPISIEGVKTFALYLYQAK
Sbjct: 601 AFNCQGGGWCRETRRNQCFSQYSKRVTSKTNAKDIEWNSGENPISIEGVKTFALYLYQAK 660

Query: 661 KLILAKPTQNIDIALDPFDFELIFVSPVTTLIETAVQFAPIGLVNMLNIGGAIQSVYYDD 720
           KLILAKPTQNIDIALDPFDFELIFVSP+TTLIETAVQFAPIGLVNMLNIGGAIQSVYYDD
Sbjct: 661 KLILAKPTQNIDIALDPFDFELIFVSPLTTLIETAVQFAPIGLVNMLNIGGAIQSVYYDD 720

Query: 721 DLSSVEIGVKGTGEMRVYASDKPRACRIDGEDVGFKYQDQMVAVQVPWPGSSGISTIEYL 730
           DLSSVEIG+KGTGEMRVYASDKPRACRIDGEDVGFKYQDQMVAVQVPWPGSSGISTIEYL
Sbjct: 721 DLSSVEIGIKGTGEMRVYASDKPRACRIDGEDVGFKYQDQMVAVQVPWPGSSGISTIEYL 780

BLAST of Carg15615-RA vs. NCBI nr
Match: XP_023526448.1 (probable galactinol--sucrose galactosyltransferase 5 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1397.1 bits (3615), Expect = 0.0e+00
Identity = 666/781 (85.28%), Postives = 702/781 (89.88%), Query Frame = 0

Query: 1   MAPILKNGNSNIVVSFDGLNDLSSPFSIDGSNFTVNGHTFLSDVPENIVASPSPYTTIDK 60
           MAP LKNG SN VVS+DGLND+S PFSID S+FTVNGH FLSDVPENIVASPSPYT+IDK
Sbjct: 1   MAPSLKNGGSNDVVSYDGLNDMSCPFSIDKSDFTVNGHLFLSDVPENIVASPSPYTSIDK 60

Query: 61  SPVSIGCFVGFDVLDPDSRHVVSIGKLKDIKFMSIFRFKVWWTTHWVGRNGGDLESETQI 120
           SPVS+GCFVGFD  +PDSRHVVSIGKLKDI+FMSIFRFKVWWTTHWVGRNGGDLESETQI
Sbjct: 61  SPVSVGCFVGFDASEPDSRHVVSIGKLKDIRFMSIFRFKVWWTTHWVGRNGGDLESETQI 120

Query: 121 VILEKSDSGRPYVLLLPLVEGPFRSSIQPGEDDFVDVCVESGSSKVVDSSFRSVLYLHA- 180
           VILEKSDSGRPYVLLLPLVEGPFR+SIQPG+DDFVDVCVESGSSKVV S+FRSVLYLHA 
Sbjct: 121 VILEKSDSGRPYVLLLPLVEGPFRTSIQPGDDDFVDVCVESGSSKVVGSTFRSVLYLHAG 180

Query: 181 ---------------------------------------------------GIMEGVKHL 240
                                                              GI+EGVKHL
Sbjct: 181 DDPFALVKEAMKIVRTHLGTFRLLEEKSPPGIVDKFGWCTWDAFYLTVHPQGIIEGVKHL 240

Query: 241 VDGGCPPGLVLIDDGWQSIGHDADPITKEGMNQTVAGEQMPCRLLKFQENYKFRDYVNPK 300
           VDGGCPPGLVLIDDGWQSIGHD+DPITKEGMNQT AGEQMPCRLLKFQENYKFRDYVNPK
Sbjct: 241 VDGGCPPGLVLIDDGWQSIGHDSDPITKEGMNQTAAGEQMPCRLLKFQENYKFRDYVNPK 300

Query: 301 SSGVGSGQKGMKAFIDELKGEYKTVEYVYVWHALCGYWGGLRPNVPGLPEANVIQPVLSP 360
           +S VG+ QKGMKAFIDELKGE+KTVEYVYVWHALCGYWGGLRP VPGLPEANVIQPVLSP
Sbjct: 301 ASSVGATQKGMKAFIDELKGEFKTVEYVYVWHALCGYWGGLRPEVPGLPEANVIQPVLSP 360

Query: 361 GLQQTMEDLAVDKIVCHKVGIVPPEKAEEMYEGLHAHLENVGIDGVKIDVIHLLEMLCED 420
           GLQ TMEDLAVDKIV HKVG+VPPEK EEMYEGLHAHLENVGIDGVKIDVIHLLEMLCED
Sbjct: 361 GLQMTMEDLAVDKIVHHKVGLVPPEKVEEMYEGLHAHLENVGIDGVKIDVIHLLEMLCED 420

Query: 421 YGGRVDLAKAYYKAMSKSIKKHFKGNGVIASMEHCNDFMFLGTEAISLGRVGDDFWCTDP 480
           YGGRVDLAKAYYKAM+KSI KHFKGNGVIASMEHCNDFMFLGT+AISLGRVGDDFWCTDP
Sbjct: 421 YGGRVDLAKAYYKAMTKSINKHFKGNGVIASMEHCNDFMFLGTQAISLGRVGDDFWCTDP 480

Query: 481 SGDPNGTFWLQGCHMVHCAYNSLWMGNFIHPDWDMFQSTHPCAAFHAASRAISGGPIYVS 540
           SGDPNGTFWLQGCHMVHCAYNSLWMGNFIHPDWDMFQSTHPCAAFHAASRAISGGPIYVS
Sbjct: 481 SGDPNGTFWLQGCHMVHCAYNSLWMGNFIHPDWDMFQSTHPCAAFHAASRAISGGPIYVS 540

Query: 541 DFVGKHNFELLRKLVLPDGSILRSEYYALPTRDCLFDDPLHDGETMLKIWNLNKFTGVIG 600
           D VGKHNFELL+KLVLPDGSILRSEYYALPTRDCLF+DPLH+GETMLKIWNLNKFTGVIG
Sbjct: 541 DSVGKHNFELLKKLVLPDGSILRSEYYALPTRDCLFEDPLHNGETMLKIWNLNKFTGVIG 600

Query: 601 AFNCQGGGWCRETRRNQCFSQYSKRVTSKTNAKDIEWNSGENPISIEGVKTFALYLYQAK 660
           AFNCQGGGWCRETRRNQCFSQYSKRVTSKTNAKDIEWNSGE PISIEGVK+FALY YQ+K
Sbjct: 601 AFNCQGGGWCRETRRNQCFSQYSKRVTSKTNAKDIEWNSGEKPISIEGVKSFALYHYQSK 660

Query: 661 KLILAKPTQNIDIALDPFDFELIFVSPVTTLIETAVQFAPIGLVNMLNIGGAIQSVYYDD 720
           KLI+AKP+Q+I+I+LDPF+FELI VSPVTTL ET++QFAPIGL+NMLN GGAIQSV Y D
Sbjct: 661 KLIIAKPSQSIEISLDPFEFELITVSPVTTLAETSIQFAPIGLINMLNTGGAIQSVVYHD 720

Query: 721 DLSSVEIGVKGTGEMRVYASDKPRACRIDGEDVGFKYQDQMVAVQVPWPGSSGISTIEYL 730
           DLSSVE+ +KGTGEMRV+AS+KPRAC IDGEDVGFKYQDQMVAVQVPWPGSSGISTIEYL
Sbjct: 721 DLSSVEVSIKGTGEMRVFASEKPRACLIDGEDVGFKYQDQMVAVQVPWPGSSGISTIEYL 780

BLAST of Carg15615-RA vs. NCBI nr
Match: XP_022934881.1 (probable galactinol--sucrose galactosyltransferase 5 [Cucurbita moschata])

HSP 1 Score: 1393.3 bits (3605), Expect = 0.0e+00
Identity = 662/781 (84.76%), Postives = 702/781 (89.88%), Query Frame = 0

Query: 1   MAPILKNGNSNIVVSFDGLNDLSSPFSIDGSNFTVNGHTFLSDVPENIVASPSPYTTIDK 60
           MAP LKNG SN VVS+DGLND+S PFSID S+FTVNGH FLSDVPENIVASPSPYT+IDK
Sbjct: 1   MAPSLKNGGSNDVVSYDGLNDMSCPFSIDKSDFTVNGHLFLSDVPENIVASPSPYTSIDK 60

Query: 61  SPVSIGCFVGFDVLDPDSRHVVSIGKLKDIKFMSIFRFKVWWTTHWVGRNGGDLESETQI 120
           SPVS+GCFVGFD  +PDSRHVVSIGKLKDI+FMSIFRFKVWWTTHWVGRNGGDLESETQI
Sbjct: 61  SPVSVGCFVGFDASEPDSRHVVSIGKLKDIRFMSIFRFKVWWTTHWVGRNGGDLESETQI 120

Query: 121 VILEKSDSGRPYVLLLPLVEGPFRSSIQPGEDDFVDVCVESGSSKVVDSSFRSVLYLHA- 180
           VILEKSDSGRPYVLLLPLVEGPFR+SIQPG+DDFVDVCVESGSSKVV S+FRSVLYLHA 
Sbjct: 121 VILEKSDSGRPYVLLLPLVEGPFRTSIQPGDDDFVDVCVESGSSKVVGSTFRSVLYLHAG 180

Query: 181 ---------------------------------------------------GIMEGVKHL 240
                                                              GI+EGVKHL
Sbjct: 181 DDPFALVKEAMKIVRTHLGTFRLLEEKSPPGIVDKFGWCTWDAFYLTVHPQGIIEGVKHL 240

Query: 241 VDGGCPPGLVLIDDGWQSIGHDADPITKEGMNQTVAGEQMPCRLLKFQENYKFRDYVNPK 300
           VDGGCPPGLVLIDDGWQSIGHD+DPITKEGMNQTVAGEQMPCRLLKFQENYKFRDYVNPK
Sbjct: 241 VDGGCPPGLVLIDDGWQSIGHDSDPITKEGMNQTVAGEQMPCRLLKFQENYKFRDYVNPK 300

Query: 301 SSGVGSGQKGMKAFIDELKGEYKTVEYVYVWHALCGYWGGLRPNVPGLPEANVIQPVLSP 360
           +S VG+ QKGMKAFIDELKGE+KTVEYVYVWHALCGYWGGLRP VPGLPEANVIQPVLSP
Sbjct: 301 ASSVGATQKGMKAFIDELKGEFKTVEYVYVWHALCGYWGGLRPEVPGLPEANVIQPVLSP 360

Query: 361 GLQQTMEDLAVDKIVCHKVGIVPPEKAEEMYEGLHAHLENVGIDGVKIDVIHLLEMLCED 420
           GLQ TMEDLAVDKIV HKVG+VPPEKAEEMYEGLHAHLENVGIDGVKIDVIHLLEMLCED
Sbjct: 361 GLQMTMEDLAVDKIVHHKVGLVPPEKAEEMYEGLHAHLENVGIDGVKIDVIHLLEMLCED 420

Query: 421 YGGRVDLAKAYYKAMSKSIKKHFKGNGVIASMEHCNDFMFLGTEAISLGRVGDDFWCTDP 480
           YGGRVDLAKAYYKAM+KSI KHFKGNGVIASMEHCNDFMFLGT+AISLGRVGDDFWCTDP
Sbjct: 421 YGGRVDLAKAYYKAMTKSINKHFKGNGVIASMEHCNDFMFLGTQAISLGRVGDDFWCTDP 480

Query: 481 SGDPNGTFWLQGCHMVHCAYNSLWMGNFIHPDWDMFQSTHPCAAFHAASRAISGGPIYVS 540
           SGDPNGTFWLQGCHMVHCAYNSLWMGNFIHPDWDMFQSTHPCAAFHAASRAISGGPIYVS
Sbjct: 481 SGDPNGTFWLQGCHMVHCAYNSLWMGNFIHPDWDMFQSTHPCAAFHAASRAISGGPIYVS 540

Query: 541 DFVGKHNFELLRKLVLPDGSILRSEYYALPTRDCLFDDPLHDGETMLKIWNLNKFTGVIG 600
           D VGKHNFELL+KLVLPDGSILRSEYYALPTRDCLF+DPLH+GETMLKIWNLNKFTGVIG
Sbjct: 541 DSVGKHNFELLKKLVLPDGSILRSEYYALPTRDCLFEDPLHNGETMLKIWNLNKFTGVIG 600

Query: 601 AFNCQGGGWCRETRRNQCFSQYSKRVTSKTNAKDIEWNSGENPISIEGVKTFALYLYQAK 660
           AFNCQGGGWCRETRRNQCFSQYSKRVTSKTNAKDIEWN GE  ISIEGVKTFALY YQ+K
Sbjct: 601 AFNCQGGGWCRETRRNQCFSQYSKRVTSKTNAKDIEWNKGEKRISIEGVKTFALYHYQSK 660

Query: 661 KLILAKPTQNIDIALDPFDFELIFVSPVTTLIETAVQFAPIGLVNMLNIGGAIQSVYYDD 720
           KL++AKP+QNI+I+LDPF+FELI VSP+T L+ET+++FAPIGL+NMLN GGAIQSV Y D
Sbjct: 661 KLVIAKPSQNIEISLDPFEFELITVSPITPLVETSIEFAPIGLINMLNTGGAIQSVVYHD 720

Query: 721 DLSSVEIGVKGTGEMRVYASDKPRACRIDGEDVGFKYQDQMVAVQVPWPGSSGISTIEYL 730
           +LSSVE+ +KGTGEMRV+AS+KPRAC IDGEDVGFKYQDQMVAVQVPWPGSSGISTI+YL
Sbjct: 721 ELSSVEVSIKGTGEMRVFASEKPRACLIDGEDVGFKYQDQMVAVQVPWPGSSGISTIQYL 780

BLAST of Carg15615-RA vs. TAIR10
Match: AT5G40390.1 (Raffinose synthase family protein)

HSP 1 Score: 1033.9 bits (2672), Expect = 4.8e-302
Identity = 499/782 (63.81%), Postives = 591/782 (75.58%), Query Frame = 0

Query: 15  SFDGLN--DLSSPFSIDGSNFTVNGHTFLSDVPENIVASPSPYTTIDKS----PVSIGCF 74
           S  G+N  D +  F ++ S    NG   L+DVP N+  + SPY  +DK      VS G F
Sbjct: 9   SDSGINGVDFTEKFRLEDSTLLANGQVVLTDVPVNVTLTSSPY-LVDKDGVPLDVSAGSF 68

Query: 75  VGFDV-LDPDSRHVVSIGKLKDIKFMSIFRFKVWWTTHWVGRNGGDLESETQIVILEK-- 134
           +GF++  +P S HV SIGKLK+I+FMSIFRFKVWWTTHWVG NG D+E+ETQI+IL++  
Sbjct: 69  IGFNLDGEPKSHHVASIGKLKNIRFMSIFRFKVWWTTHWVGSNGRDIENETQIIILDQSG 128

Query: 135 ------SDSGRPYVLLLPLVEGPFRSSIQPGEDDFVDVCVESGSSKVVDSSFRSVLYLHA 194
                 S SGRPYVLLLPL+EG FRSS Q GEDD V VCVESGS++V  S FR ++Y+HA
Sbjct: 129 SDSGPGSGSGRPYVLLLPLLEGSFRSSFQSGEDDDVAVCVESGSTEVTGSEFRQIVYVHA 188

Query: 195 ----------------------------------------------------GIMEGVKH 254
                                                               G+ +GVK 
Sbjct: 189 GDDPFKLVKDAMKVIRVHMNTFKLLEEKSPPGIVDKFGWCTWDAFYLTVNPDGVHKGVKC 248

Query: 255 LVDGGCPPGLVLIDDGWQSIGHDADPITKEGMNQTVAGEQMPCRLLKFQENYKFRDYVNP 314
           LVDGGCPPGLVLIDDGWQSIGHD+D I  EGMN TVAGEQMPCRLLKF+EN+KF+DYV+P
Sbjct: 249 LVDGGCPPGLVLIDDGWQSIGHDSDGIDVEGMNITVAGEQMPCRLLKFEENHKFKDYVSP 308

Query: 315 KSSGVGSGQKGMKAFIDELKGEYKTVEYVYVWHALCGYWGGLRPNVPGLPEANVIQPVLS 374
           K         GMKAF+ +LK E+ TV+Y+YVWHALCGYWGGLRP  P LP + +I+P LS
Sbjct: 309 KD----QNDVGMKAFVRDLKDEFSTVDYIYVWHALCGYWGGLRPEAPALPPSTIIRPELS 368

Query: 375 PGLQQTMEDLAVDKIVCHKVGIVPPEKAEEMYEGLHAHLENVGIDGVKIDVIHLLEMLCE 434
           PGL+ TMEDLAVDKI+   +G   P+ A+E YEGLH+HL+N GIDGVK+DVIH+LEMLC+
Sbjct: 369 PGLKLTMEDLAVDKIIETGIGFASPDLAKEFYEGLHSHLQNAGIDGVKVDVIHILEMLCQ 428

Query: 435 DYGGRVDLAKAYYKAMSKSIKKHFKGNGVIASMEHCNDFMFLGTEAISLGRVGDDFWCTD 494
            YGGRVDLAKAY+KA++ S+ KHF GNGVIASMEHCNDFMFLGTEAISLGRVGDDFWCTD
Sbjct: 429 KYGGRVDLAKAYFKALTSSVNKHFNGNGVIASMEHCNDFMFLGTEAISLGRVGDDFWCTD 488

Query: 495 PSGDPNGTFWLQGCHMVHCAYNSLWMGNFIHPDWDMFQSTHPCAAFHAASRAISGGPIYV 554
           PSGDPNGTFWLQGCHMVHCAYNSLWMGNFI PDWDMFQSTHPCA FHAASRAISGGPIY+
Sbjct: 489 PSGDPNGTFWLQGCHMVHCAYNSLWMGNFIQPDWDMFQSTHPCAEFHAASRAISGGPIYI 548

Query: 555 SDFVGKHNFELLRKLVLPDGSILRSEYYALPTRDCLFDDPLHDGETMLKIWNLNKFTGVI 614
           SD VGKH+F+LL++LVLP+GSILR EYYALPTRD LF+DPLHDG+TMLKIWNLNK+TGVI
Sbjct: 549 SDCVGKHDFDLLKRLVLPNGSILRCEYYALPTRDRLFEDPLHDGKTMLKIWNLNKYTGVI 608

Query: 615 GAFNCQGGGWCRETRRNQCFSQYSKRVTSKTNAKDIEWNSGENPISIEGVKTFALYLYQA 674
           GAFNCQGGGWCRETRRNQCFS+    +T+ T+ KD+EWNSG +PISI  V+ FAL+L Q+
Sbjct: 609 GAFNCQGGGWCRETRRNQCFSECVNTLTATTSPKDVEWNSGSSPISIANVEEFALFLSQS 668

Query: 675 KKLILAKPTQNIDIALDPFDFELIFVSPVTTLIETAVQFAPIGLVNMLNIGGAIQSVYYD 730
           KKL+L+    ++++ L+PF FELI VSPV T+   +V+FAPIGLVNMLN  GAI+S+ Y+
Sbjct: 669 KKLLLSGLNDDLELTLEPFKFELITVSPVVTIEGNSVRFAPIGLVNMLNTSGAIRSLVYN 728

BLAST of Carg15615-RA vs. TAIR10
Match: AT4G01970.1 (stachyose synthase)

HSP 1 Score: 610.9 bits (1574), Expect = 1.0e-174
Identity = 331/841 (39.36%), Postives = 462/841 (54.93%), Query Frame = 0

Query: 29  DGSNFTVNGHTFLSDVPENIVASP-SPYTTIDKSPVSI----------GCFVGFDVLDPD 88
           +GS    +    L DVP+N+  +P S ++    +P+ I          G F+GF    P 
Sbjct: 38  EGSLCAKDSTPILFDVPQNVTFTPFSSHSISTDAPLPILLRVQANAHKGGFLGFTKESPS 97

Query: 89  SRHVVSIGKLKDIKFMSIFRFKVWWTTHWVGRNGGDLESETQIVILEKSDSGRPYVLLLP 148
            R   S+G+ +D +F+S+FRFK+WW+T W+G++G DL++ETQ V+L K      YV ++P
Sbjct: 98  DRLTNSLGRFEDREFLSLFRFKMWWSTAWIGKSGSDLQAETQWVML-KIPEIDSYVAIIP 157

Query: 149 LVEGPFRSSIQPGEDDFVDVCVESGSSKVVDSSFRSVLYLH------------------- 208
            +EG FR+S+ PGE   V +C ESGS+KV +SSF+S+ Y+H                   
Sbjct: 158 TIEGAFRASLTPGEKGNVLICAESGSTKVKESSFKSIAYIHICDNPYNLMKEAFSALRVH 217

Query: 209 ---------------------------------AGIMEGVKHLVDGGCPPGLVLIDDGWQ 268
                                            A I  GVK   DGG  P  V+IDDGWQ
Sbjct: 218 MNTFKLLEEKKLPKIVDKFGWCTWDACYLTVDPATIWTGVKEFEDGGVCPKFVIIDDGWQ 277

Query: 269 SIGHDADPITKEGMNQTVAGEQMPCRLLKFQENYKFRDY------------VNP------ 328
           SI  D D + K+  N  + GEQM  RL  F+E  KFR+Y             NP      
Sbjct: 278 SINFDGDELDKDAENLVLGGEQMTARLTSFKECKKFRNYKGGSFITSDASHFNPLKPKML 337

Query: 329 ------------------------------------------------KSSGVGSGQ--- 388
                                                           K   +GS     
Sbjct: 338 IYKATERIQAIILRRKLVKESGEQDLTELDEKIKILSEELNAMFDEVEKEESLGSDDVSG 397

Query: 389 KGMKAFIDELKGEYKTVEYVYVWHALCGYWGGLRPNVPGLPEANVIQPVLSPGLQQTMED 448
            GM AF  +L+  +K+++ +YVWHALCG W G+RP      +A V    LSP L  TM D
Sbjct: 398 SGMAAFTKDLRLRFKSLDDIYVWHALCGAWNGVRPETMMDLKAKVAPFELSPSLGATMAD 457

Query: 449 LAVDKIVCHKVGIVPPEKAEEMYEGLHAHLENVGIDGVKIDVIHLLEMLCEDYGGRVDLA 508
           LAVDK+V   +G+V P KA E Y+ +H++L +VG+ G KIDV   LE L E++GGRV+LA
Sbjct: 458 LAVDKVVEAGIGLVHPSKAHEFYDSMHSYLASVGVTGAKIDVFQTLESLAEEHGGRVELA 517

Query: 509 KAYYKAMSKSIKKHFKGNGVIASMEHCNDFMFLGTEAISLGRVGDDFWCTDPSGDPNGTF 568
           KAYY  +++S+ K+F G  VIASM+ CN+F FL T+ IS+GRVGDDFW  DP GDP G +
Sbjct: 518 KAYYDGLTESMIKNFNGTDVIASMQQCNEFFFLATKQISIGRVGDDFWWQDPYGDPQGVY 577

Query: 569 WLQGCHMVHCAYNSLWMGNFIHPDWDMFQSTHPCAAFHAASRAISGGPIYVSDFVGK--H 628
           WLQG HM+HC+YNS+WMG  I PDWDMFQS H CA +HAASRAI GGP+Y+SD +GK  H
Sbjct: 578 WLQGVHMIHCSYNSIWMGQMIQPDWDMFQSDHVCAEYHAASRAICGGPVYLSDHLGKASH 637

Query: 629 NFELLRKLVLPDGSILRSEYYALPTRDCLFDDPLHDGETMLKIWNLNKFTGVIGAFNCQG 688
           NF+L++KL   DG+I R  +YALPTRD LF +PL D E++LKI+N NKF GVIG FNCQG
Sbjct: 638 NFDLIKKLAFFDGTIPRCVHYALPTRDSLFKNPLFDKESILKIFNFNKFGGVIGTFNCQG 697

Query: 689 GGWCRETRRNQCFSQYSKRVTSKTNAKDIEWNSGENPISIEGVKT--FALYLYQAKK-LI 730
            GW  E  R + + +    V+   +  DIEW+        +   T  + +Y  Q+++ L 
Sbjct: 698 AGWSPEEHRFKGYKECYTTVSGTVHVSDIEWDQNPEAAGSQVTYTGDYLVYKQQSEEILF 757

BLAST of Carg15615-RA vs. TAIR10
Match: AT3G57520.1 (seed imbibition 2)

HSP 1 Score: 488.4 bits (1256), Expect = 7.6e-138
Identity = 273/786 (34.73%), Postives = 408/786 (51.91%), Query Frame = 0

Query: 22  LSSPFSIDGSNFTVNGHTFLSDVPENIVASPSPYTTIDKSPVSIGCFVGFDVLDPDSRHV 81
           ++S  S+   N  V G T L+ +P+NI+ +P     +  +    G F+G       S HV
Sbjct: 3   ITSNISVQNDNLVVQGKTILTKIPDNIILTP-----VTGNGFVSGSFIGATFEQSKSLHV 62

Query: 82  VSIGKLKDIKFMSIFRFKVWWTTHWVGRNGGDLESETQIVILEK--------SDSGRPYV 141
             IG L+ ++FM  FRFK+WW T  +G  G D+  ETQ ++LE          D+   Y 
Sbjct: 63  FPIGVLEGLRFMCCFRFKLWWMTQRMGSCGKDIPLETQFMLLESKDEVEGNGDDAPTVYT 122

Query: 142 LLLPLVEGPFRSSIQPGEDDFVDVCVESGSSKVVDSSFRSVLYLHA-------------- 201
           + LPL+EG FR+ +Q  E + +++C ESG   V  S    ++Y+HA              
Sbjct: 123 VFLPLLEGQFRAVLQGNEKNEIEICFESGDKAVETSQGTHLVYVHAGTNPFEVIRQSVKA 182

Query: 202 --------------------------------------GIMEGVKHLVDGGCPPGLVLID 261
                                                 G+ EG+K L +GG PP  ++ID
Sbjct: 183 VERHMQTFHHREKKKLPSFLDWFGWCTWDAFYTDVTAEGVDEGLKSLSEGGTPPKFLIID 242

Query: 262 DGWQSIGH---DADPITKEGMNQTVAGEQMPCRLLKFQENYKFRDYVNPKSSGVGSGQKG 321
           DGWQ I +   D + + +E       G Q   RL+  +EN KF+     KS    +   G
Sbjct: 243 DGWQQIENKEKDENCVVQE-------GAQFATRLVGIKENAKFQ-----KSDQKDTQVSG 302

Query: 322 MKAFIDELKGEYKTVEYVYVWHALCGYWGGLRPNVPGLP--EANVIQPVLSPGLQQTMED 381
           +K+ +D  K  +  V+ VY WHAL GYWGG++P   G+   ++ +  PV SPG+     D
Sbjct: 303 LKSVVDNAKQRH-NVKQVYAWHALAGYWGGVKPAASGMEHYDSALAYPVQSPGVLGNQPD 362

Query: 382 LAVDKIVCHKVGIVPPEKAEEMYEGLHAHLENVGIDGVKIDVIHLLEMLCEDYGGRVDLA 441
           + +D +  H +G+V P+K    Y  LH++L + GIDGVK+DV +++E L    GGRV L 
Sbjct: 363 IVMDSLAVHGLGLVNPKKVFNFYNELHSYLASCGIDGVKVDVQNIIETLGAGLGGRVSLT 422

Query: 442 KAYYKAMSKSIKKHFKGNGVIASMEHCNDFMFLGTEAISLGRVGDDFWCTDPSGDPNGTF 501
           ++Y +A+  SI ++F  NG I+ M H  D ++   +  ++ R  DDF+  DP+       
Sbjct: 423 RSYQQALEASIARNFTDNGCISCMCHNTDGLY-SAKQTAIVRASDDFYPRDPAS------ 482

Query: 502 WLQGCHMVHCAYNSLWMGNFIHPDWDMFQSTHPCAAFHAASRAISGGPIYVSDFVGKHNF 561
                H+   AYNSL++G F+ PDWDMF S HP A +HAA+RA+ G  IYVSD  G HNF
Sbjct: 483 --HTIHIASVAYNSLFLGEFMQPDWDMFHSLHPTAEYHAAARAVGGCAIYVSDKPGNHNF 542

Query: 562 ELLRKLVLPDGSILRSEYYALPTRDCLFDDPLHDGETMLKIWNLNKFTGVIGAFNCQGGG 621
           +LLRKLVLPDGS+LR++    PTRDCLF DP  DG ++LKIWN+NKFTG++G FNCQG G
Sbjct: 543 DLLRKLVLPDGSVLRAKLPGRPTRDCLFADPARDGISLLKIWNMNKFTGIVGVFNCQGAG 602

Query: 622 WCRETRRNQCFSQYSKRVTSKTNAKDIEWNSGENPISIEGVKTFALYLYQAKKLILAKPT 681
           WC+ET++NQ        +T    A D +  S        G     +Y Y++ +++     
Sbjct: 603 WCKETKKNQIHDTSPGTLTGSIRADDADLISQVAGEDWSG--DSIVYAYRSGEVVRLPKG 662

Query: 682 QNIDIALDPFDFELIFVSPVTTLIETAVQFAPIGLVNMLNIGGAIQSV-----------Y 718
            +I + L   ++EL  +SP+  + E  + FAPIGLV+M N  GAI+S+           +
Sbjct: 663 ASIPLTLKVLEYELFHISPLKEITEN-ISFAPIGLVDMFNSSGAIESIDINHVTDKNPEF 722

BLAST of Carg15615-RA vs. TAIR10
Match: AT5G20250.4 (Raffinose synthase family protein)

HSP 1 Score: 482.3 bits (1240), Expect = 5.4e-136
Identity = 276/766 (36.03%), Postives = 402/766 (52.48%), Query Frame = 0

Query: 28  IDGSNFTVNGHTFLSDVPENIVASPSPYTTIDKSPVSIGCFVGFDVLDPDSRHVVSIGKL 87
           I   N  +   T L+ VP+N++ +    +  +  PV  G FVG      +S+H+V IG L
Sbjct: 104 ISDGNLIIKNRTILTGVPDNVITT----SASEAGPVE-GVFVGAVFNKEESKHIVPIGTL 163

Query: 88  KDIKFMSIFRFKVWWTTHWVGRNGGDLESETQIVILEKSDSG--------------RPYV 147
           ++ +FMS FRFK+WW    +G  G D+  ETQ +++E +D                + Y 
Sbjct: 164 RNSRFMSCFRFKLWWMAQRMGEMGRDIPYETQFLLVESNDGSHLESDGANGVECNQKVYT 223

Query: 148 LLLPLVEGPFRSSIQPGEDDFVDVCVESGSSKVVDSSFRSVLYLHA-------------- 207
           + LPL+EG FRS +Q   +D V++C+ESG      SSF   LY+HA              
Sbjct: 224 VFLPLIEGSFRSCLQGNVNDEVELCLESGDVDTKRSSFTHSLYIHAGTDPFQTITDAIRT 283

Query: 208 --------------------------------------GIMEGVKHLVDGGCPPGLVLID 267
                                                 G+  G+K L  GG PP  V+ID
Sbjct: 284 VKLHLNSFRQRHEKKLPGIVDYFGWCTWDAFYQEVTQEGVEAGLKSLAAGGTPPKFVIID 343

Query: 268 DGWQSIGHDADPITKEGMNQTVAGEQMPCRLLKFQENYKFRDYVNPKSSGVGSGQKGMKA 327
           DGWQS+  DA   T E  ++    E    RL   +EN KF+   +P          G+K 
Sbjct: 344 DGWQSVERDA---TVEAGDE--KKESPIFRLTGIKENEKFKKKDDP--------NVGIKN 403

Query: 328 FIDELKGEYKTVEYVYVWHALCGYWGGLRPNVPGLPEANVIQ-PVLSPGLQQTMEDLAVD 387
            + ++  E   ++YVYVWHA+ GYWGG+R   PG    +V++ P +S G+ +       D
Sbjct: 404 IV-KIAKEKHGLKYVYVWHAITGYWGGVR---PGEEYGSVMKYPNMSKGVVENDPTWKTD 463

Query: 388 KIVCHKVGIVPPEKAEEMYEGLHAHLENVGIDGVKIDVIHLLEMLCEDYGGRVDLAKAYY 447
            +    +G+V P+K  + Y  LH++L + G+DGVK+DV  +LE L    GGRV+L + ++
Sbjct: 464 VMTLQGLGLVSPKKVYKFYNELHSYLADAGVDGVKVDVQCVLETLGGGLGGRVELTRQFH 523

Query: 448 KAMSKSIKKHFKGNGVIASMEHCNDFMFLGTEAISLGRVGDDFWCTDPSGDPNGTFWLQG 507
           +A+  S+ K+F  NG IA M H  D ++   +A  + R  DDF+  DP            
Sbjct: 524 QALDSSVAKNFPDNGCIACMSHNTDALYCSKQAAVI-RASDDFYPRDPVS--------HT 583

Query: 508 CHMVHCAYNSLWMGNFIHPDWDMFQSTHPCAAFHAASRAISGGPIYVSDFVGKHNFELLR 567
            H+   AYNS+++G F+ PDWDMF S HP A +HA++RAISGGP+YVSD  GKHNFELLR
Sbjct: 584 IHIASVAYNSVFLGEFMQPDWDMFHSVHPAAEYHASARAISGGPLYVSDSPGKHNFELLR 643

Query: 568 KLVLPDGSILRSEYYALPTRDCLFDDPLHDGETMLKIWNLNKFTGVIGAFNCQGGGWCRE 627
           KLVLPDGSILR+     PTRDCLF DP  DG ++LKIWN+NK+TGV+G +NCQG  W   
Sbjct: 644 KLVLPDGSILRARLPGRPTRDCLFADPARDGVSLLKIWNMNKYTGVLGVYNCQGAAWSST 703

Query: 628 TRRNQCFSQYSKRVTSKTNAKDIEWNSGENPISIEGVKTFALYLYQAKKLILAKPTQNID 687
            R+N      +  +T     +D+   S  +          A+Y     +LI+     ++ 
Sbjct: 704 ERKNIFHQTKTDSLTGSIRGRDVHSISEASTDPTTWNGDCAVYSQSRGELIVMPYNVSLP 763

Query: 688 IALDPFDFELIFVSPVTTLIETAVQFAPIGLVNMLNIGGAIQSVYYDDDLSSVEIGVKGT 727
           ++L   + E+  VSP++ L++  V FAPIGLVNM N GGAI+ + Y+ +   V + VKG 
Sbjct: 764 VSLKIREHEIFTVSPISHLVD-GVSFAPIGLVNMYNSGGAIEGLRYEAEKMKVVMEVKGC 823

BLAST of Carg15615-RA vs. TAIR10
Match: AT1G55740.1 (seed imbibition 1)

HSP 1 Score: 474.6 bits (1220), Expect = 1.1e-133
Identity = 264/748 (35.29%), Postives = 402/748 (53.74%), Query Frame = 0

Query: 27  SIDGSNFTVNGHTFLSDVPENIVASP-SPYTTIDKSPVSIGCFVGFDVLDPDSRHVVSIG 86
           S+  S+  V GH  L  VPEN++ +P S    ID      G F+G       S  V S+G
Sbjct: 8   SVTDSDLVVLGHRVLHGVPENVLVTPASGNALID------GAFIGVTSDQTGSHRVFSLG 67

Query: 87  KLKDIKFMSIFRFKVWWTTHWVGRNGGDLESETQIVILEKS--------DSGRPYVLLLP 146
           KL+D++FM +FRFK+WW T  +G NG ++  ETQ +I+E +        D    YV+ LP
Sbjct: 68  KLEDLRFMCVFRFKLWWMTQRMGTNGKEIPCETQFLIVEANQGSDLGGRDQSSSYVVFLP 127

Query: 147 LVEGPFRSSIQPGEDDFVDVCVESGSSKVVDSSFRSVLYLHAG----------------- 206
           ++EG FR+ +Q  E + +++C+ESG   V       ++++ AG                 
Sbjct: 128 ILEGDFRAVLQGNEANELEICLESGDPTVDQFEGSHLVFVAAGSDPFDVITKAVKAVEQH 187

Query: 207 -----------------------------------IMEGVKHLVDGGCPPGLVLIDDGWQ 266
                                              + +G++ L  GG  P  V+IDDGWQ
Sbjct: 188 LQTFSHRERKKMPDMLNWFGWCTWDAFYTNVTAKDVKQGLESLKAGGVTPKFVIIDDGWQ 247

Query: 267 SIGHDADPITKEGMNQTVAGEQMPCRLLKFQENYKFRDYVNPKSSGVGSGQKGMKAFIDE 326
           S+G D   +     N          RL   +EN+KF+     +   V      +   I +
Sbjct: 248 SVGMDETSVEFNADN----AANFANRLTHIKENHKFQK-DGKEGHRVDDPSLSLGHVITD 307

Query: 327 LKGEYKTVEYVYVWHALCGYWGGLRPNVPGLP--EANVIQPVLSPGLQQTMEDLAVDKIV 386
           +K    +++YVYVWHA+ GYWGG++P V G+   E+ V  PV SPG+  +     ++ I 
Sbjct: 308 IKSN-NSLKYVYVWHAITGYWGGVKPGVSGMEHYESKVAYPVSSPGVMSSENCGCLESIT 367

Query: 387 CHKVGIVPPEKAEEMYEGLHAHLENVGIDGVKIDVIHLLEMLCEDYGGRVDLAKAYYKAM 446
            + +G+V PEK    Y  LH++L +VG+DGVK+DV ++LE L   +GGRV LAK Y++A+
Sbjct: 368 KNGLGLVNPEKVFSFYNDLHSYLASVGVDGVKVDVQNILETLGAGHGGRVKLAKKYHQAL 427

Query: 447 SKSIKKHFKGNGVIASMEHCNDFMFLGTEAISLGRVGDDFWCTDPSGDPNGTFWLQGCHM 506
             SI ++F  NG+I+ M H  D ++   +  ++ R  DDFW  DP+            H+
Sbjct: 428 EASISRNFPDNGIISCMSHNTDGLY-SAKKTAVIRASDDFWPRDPAS--------HTIHI 487

Query: 507 VHCAYNSLWMGNFIHPDWDMFQSTHPCAAFHAASRAISGGPIYVSDFVGKHNFELLRKLV 566
              AYN+L++G F+ PDWDMF S HP A +HAA+RA+ G  IYVSD  G+H+F LLRKLV
Sbjct: 488 ASVAYNTLFLGEFMQPDWDMFHSLHPMAEYHAAARAVGGCAIYVSDKPGQHDFNLLRKLV 547

Query: 567 LPDGSILRSEYYALPTRDCLFDDPLHDGETMLKIWNLNKFTGVIGAFNCQGGGWCRETRR 626
           L DGSILR++    PT DC F DP+ D +++LKIWNLN+FTGVIG FNCQG GWC+  +R
Sbjct: 548 LRDGSILRAKLPGRPTSDCFFSDPVRDNKSLLKIWNLNEFTGVIGVFNCQGAGWCKNEKR 607

Query: 627 NQCFSQYSKRVTSKTNAKDIEWNSGENPISIEGVKTFALYLYQAKKLILAKPTQNIDIAL 686
                Q    ++      D+ +       + E      +Y +   +L+      ++ + L
Sbjct: 608 YLIHDQEPGTISGCVRTNDVHYL--HKVAAFEWTGDSIVYSHLRGELVYLPKDTSLPVTL 667

Query: 687 DPFDFELIFVSPVTTLIETAVQFAPIGLVNMLNIGGAIQSVYYDDDLSS--VEIGVKGTG 709
            P ++E+  V PV    + + +FAP+GL+ M N GGAI S+ YDD+ +   V + ++G+G
Sbjct: 668 MPREYEVFTVVPVKEFSDGS-KFAPVGLMEMFNSGGAIVSLRYDDEGTKFVVRMKLRGSG 727

BLAST of Carg15615-RA vs. Swiss-Prot
Match: sp|Q9FND9|RFS5_ARATH (Probable galactinol--sucrose galactosyltransferase 5 OS=Arabidopsis thaliana OX=3702 GN=RFS5 PE=1 SV=1)

HSP 1 Score: 1033.9 bits (2672), Expect = 8.7e-301
Identity = 499/782 (63.81%), Postives = 591/782 (75.58%), Query Frame = 0

Query: 15  SFDGLN--DLSSPFSIDGSNFTVNGHTFLSDVPENIVASPSPYTTIDKS----PVSIGCF 74
           S  G+N  D +  F ++ S    NG   L+DVP N+  + SPY  +DK      VS G F
Sbjct: 9   SDSGINGVDFTEKFRLEDSTLLANGQVVLTDVPVNVTLTSSPY-LVDKDGVPLDVSAGSF 68

Query: 75  VGFDV-LDPDSRHVVSIGKLKDIKFMSIFRFKVWWTTHWVGRNGGDLESETQIVILEK-- 134
           +GF++  +P S HV SIGKLK+I+FMSIFRFKVWWTTHWVG NG D+E+ETQI+IL++  
Sbjct: 69  IGFNLDGEPKSHHVASIGKLKNIRFMSIFRFKVWWTTHWVGSNGRDIENETQIIILDQSG 128

Query: 135 ------SDSGRPYVLLLPLVEGPFRSSIQPGEDDFVDVCVESGSSKVVDSSFRSVLYLHA 194
                 S SGRPYVLLLPL+EG FRSS Q GEDD V VCVESGS++V  S FR ++Y+HA
Sbjct: 129 SDSGPGSGSGRPYVLLLPLLEGSFRSSFQSGEDDDVAVCVESGSTEVTGSEFRQIVYVHA 188

Query: 195 ----------------------------------------------------GIMEGVKH 254
                                                               G+ +GVK 
Sbjct: 189 GDDPFKLVKDAMKVIRVHMNTFKLLEEKSPPGIVDKFGWCTWDAFYLTVNPDGVHKGVKC 248

Query: 255 LVDGGCPPGLVLIDDGWQSIGHDADPITKEGMNQTVAGEQMPCRLLKFQENYKFRDYVNP 314
           LVDGGCPPGLVLIDDGWQSIGHD+D I  EGMN TVAGEQMPCRLLKF+EN+KF+DYV+P
Sbjct: 249 LVDGGCPPGLVLIDDGWQSIGHDSDGIDVEGMNITVAGEQMPCRLLKFEENHKFKDYVSP 308

Query: 315 KSSGVGSGQKGMKAFIDELKGEYKTVEYVYVWHALCGYWGGLRPNVPGLPEANVIQPVLS 374
           K         GMKAF+ +LK E+ TV+Y+YVWHALCGYWGGLRP  P LP + +I+P LS
Sbjct: 309 KD----QNDVGMKAFVRDLKDEFSTVDYIYVWHALCGYWGGLRPEAPALPPSTIIRPELS 368

Query: 375 PGLQQTMEDLAVDKIVCHKVGIVPPEKAEEMYEGLHAHLENVGIDGVKIDVIHLLEMLCE 434
           PGL+ TMEDLAVDKI+   +G   P+ A+E YEGLH+HL+N GIDGVK+DVIH+LEMLC+
Sbjct: 369 PGLKLTMEDLAVDKIIETGIGFASPDLAKEFYEGLHSHLQNAGIDGVKVDVIHILEMLCQ 428

Query: 435 DYGGRVDLAKAYYKAMSKSIKKHFKGNGVIASMEHCNDFMFLGTEAISLGRVGDDFWCTD 494
            YGGRVDLAKAY+KA++ S+ KHF GNGVIASMEHCNDFMFLGTEAISLGRVGDDFWCTD
Sbjct: 429 KYGGRVDLAKAYFKALTSSVNKHFNGNGVIASMEHCNDFMFLGTEAISLGRVGDDFWCTD 488

Query: 495 PSGDPNGTFWLQGCHMVHCAYNSLWMGNFIHPDWDMFQSTHPCAAFHAASRAISGGPIYV 554
           PSGDPNGTFWLQGCHMVHCAYNSLWMGNFI PDWDMFQSTHPCA FHAASRAISGGPIY+
Sbjct: 489 PSGDPNGTFWLQGCHMVHCAYNSLWMGNFIQPDWDMFQSTHPCAEFHAASRAISGGPIYI 548

Query: 555 SDFVGKHNFELLRKLVLPDGSILRSEYYALPTRDCLFDDPLHDGETMLKIWNLNKFTGVI 614
           SD VGKH+F+LL++LVLP+GSILR EYYALPTRD LF+DPLHDG+TMLKIWNLNK+TGVI
Sbjct: 549 SDCVGKHDFDLLKRLVLPNGSILRCEYYALPTRDRLFEDPLHDGKTMLKIWNLNKYTGVI 608

Query: 615 GAFNCQGGGWCRETRRNQCFSQYSKRVTSKTNAKDIEWNSGENPISIEGVKTFALYLYQA 674
           GAFNCQGGGWCRETRRNQCFS+    +T+ T+ KD+EWNSG +PISI  V+ FAL+L Q+
Sbjct: 609 GAFNCQGGGWCRETRRNQCFSECVNTLTATTSPKDVEWNSGSSPISIANVEEFALFLSQS 668

Query: 675 KKLILAKPTQNIDIALDPFDFELIFVSPVTTLIETAVQFAPIGLVNMLNIGGAIQSVYYD 730
           KKL+L+    ++++ L+PF FELI VSPV T+   +V+FAPIGLVNMLN  GAI+S+ Y+
Sbjct: 669 KKLLLSGLNDDLELTLEPFKFELITVSPVVTIEGNSVRFAPIGLVNMLNTSGAIRSLVYN 728

BLAST of Carg15615-RA vs. Swiss-Prot
Match: sp|Q8VWN6|RFS_PEA (Galactinol--sucrose galactosyltransferase OS=Pisum sativum OX=3888 GN=RFS PE=1 SV=1)

HSP 1 Score: 986.1 bits (2548), Expect = 2.1e-286
Identity = 476/802 (59.35%), Postives = 588/802 (73.32%), Query Frame = 0

Query: 1   MAP--ILKNGNSNIVVS-FDGLNDLSSPFSIDGS-NFTVNGHTFLSDVPENI----VASP 60
           MAP  I K      V+S  D  N      S+D S NF VNGH FL+ VP NI     ++P
Sbjct: 1   MAPPSITKTATQQDVISTVDIGNSPLLSISLDQSRNFLVNGHPFLTQVPPNITTTTTSTP 60

Query: 61  SPYTTIDKSPVSI----------GCFVGFDVLDPDSRHVVSIGKLKDIKFMSIFRFKVWW 120
           SP+     +  +I          GCFVGF+  +  S HVV +GKLK IKF SIFRFKVWW
Sbjct: 61  SPFLDFKSNKDTIANNNNTLQQQGCFVGFNTTEAKSHHVVPLGKLKGIKFTSIFRFKVWW 120

Query: 121 TTHWVGRNGGDLESETQIVILEKSDS-GRPYVLLLPLVEGPFRSSIQPGEDDFVDVCVES 180
           TTHWVG NG +L+ ETQI+IL+K+ S GRPYVLLLP++E  FR+S+QPG +D+VD+ VES
Sbjct: 121 TTHWVGTNGHELQHETQILILDKNISLGRPYVLLLPILENSFRTSLQPGLNDYVDMSVES 180

Query: 181 GSSKVVDSSFRSVLYLH------------------------------------------- 240
           GS+ V  S+F++ LYLH                                           
Sbjct: 181 GSTHVTGSTFKACLYLHLSNDPYRLVKEAVKVIQTKLGTFKTLEEKTPPSIIEKFGWCTW 240

Query: 241 ---------AGIMEGVKHLVDGGCPPGLVLIDDGWQSIGH-DADPIT-KEGMNQTVAGEQ 300
                     G+ EGVK L DGGCPPG V+IDDGWQSI H D DP+T ++GMN+T AGEQ
Sbjct: 241 DAFYLKVHPKGVWEGVKALTDGGCPPGFVIIDDGWQSISHDDDDPVTERDGMNRTSAGEQ 300

Query: 301 MPCRLLKFQENYKFRDYVNPKSSGVGSGQKGMKAFIDELKGEYKTVEYVYVWHALCGYWG 360
           MPCRL+K++ENYKFR+Y N    G   G+KG+  F+ +LK E+++VE VYVWHALCGYWG
Sbjct: 301 MPCRLIKYEENYKFREYEN----GDNGGKKGLVGFVRDLKEEFRSVESVYVWHALCGYWG 360

Query: 361 GLRPNVPGLPEANVIQPVLSPGLQQTMEDLAVDKIVCHKVGIVPPEKAEEMYEGLHAHLE 420
           G+RP V G+PEA V+ P LSPG++ TMEDLAVDKIV + VG+VPP  A+EM++G+H+HLE
Sbjct: 361 GVRPKVCGMPEAKVVVPKLSPGVKMTMEDLAVDKIVENGVGLVPPNLAQEMFDGIHSHLE 420

Query: 421 NVGIDGVKIDVIHLLEMLCEDYGGRVDLAKAYYKAMSKSIKKHFKGNGVIASMEHCNDFM 480
           + GIDGVK+DVIHLLE+L E+YGGRV+LAKAYYKA++ S+ KHFKGNGVIASMEHCNDF 
Sbjct: 421 SAGIDGVKVDVIHLLELLSEEYGGRVELAKAYYKALTSSVNKHFKGNGVIASMEHCNDFF 480

Query: 481 FLGTEAISLGRVGDDFWCTDPSGDPNGTFWLQGCHMVHCAYNSLWMGNFIHPDWDMFQST 540
            LGTEAISLGRVGDDFWC DPSGDPNGT+WLQGCHMVHCAYNSLWMGNFIHPDWDMFQST
Sbjct: 481 LLGTEAISLGRVGDDFWCCDPSGDPNGTYWLQGCHMVHCAYNSLWMGNFIHPDWDMFQST 540

Query: 541 HPCAAFHAASRAISGGPIYVSDFVGKHNFELLRKLVLPDGSILRSEYYALPTRDCLFDDP 600
           HPCA FHAASRAISGGP+YVSD VG HNF+LL+  VLPDGSILR ++YALPTRDCLF+DP
Sbjct: 541 HPCAEFHAASRAISGGPVYVSDCVGNHNFKLLKSFVLPDGSILRCQHYALPTRDCLFEDP 600

Query: 601 LHDGETMLKIWNLNKFTGVIGAFNCQGGGWCRETRRNQCFSQYSKRVTSKTNAKDIEWNS 660
           LH+G+TMLKIWNLNK+ GV+G FNCQGGGWC ETRRN+  S++S  VT   + +DIEW +
Sbjct: 601 LHNGKTMLKIWNLNKYAGVLGLFNCQGGGWCPETRRNKSASEFSHAVTCYASPEDIEWCN 660

Query: 661 GENPISIEGVKTFALYLYQAKKLILAKPTQNIDIALDPFDFELIFVSPVTTLIETAVQFA 720
           G+ P+ I+GV  FA+Y ++ KKL L K +  ++++L+PF FEL+ VSP+    +  +QFA
Sbjct: 661 GKTPMDIKGVDVFAVYFFKEKKLSLMKCSDRLEVSLEPFSFELMTVSPLKVFSKRLIQFA 720

Query: 721 PIGLVNMLNIGGAIQSVYYDDDLSSVEIGVKGTGEMRVYASDKPRACRIDGEDVGFKYQD 730
           PIGLVNMLN GGA+QS+ +DD  S V+IGV+G GE+ V+AS+KP  C+IDG  V F Y+D
Sbjct: 721 PIGLVNMLNSGGAVQSLEFDDSASLVKIGVRGCGELSVFASEKPVCCKIDGVSVEFDYED 780

BLAST of Carg15615-RA vs. Swiss-Prot
Match: sp|Q5VQG4|RFS_ORYSJ (Galactinol--sucrose galactosyltransferase OS=Oryza sativa subsp. japonica OX=39947 GN=RFS PE=1 SV=1)

HSP 1 Score: 926.8 bits (2394), Expect = 1.5e-268
Identity = 457/803 (56.91%), Postives = 571/803 (71.11%), Query Frame = 0

Query: 1   MAPILKNGNSNI---VVSFDGLNDLSSP-FSIDGSNFTVNGHTFLSDVPENIVASPSPYT 60
           MAP L     ++   VV+ DGL  +  P F++ G +  V+GH FL DVP NI  +P+  T
Sbjct: 1   MAPNLSKAKDDLIGDVVAVDGL--IKPPRFTLKGKDLAVDGHPFLLDVPANIRLTPAS-T 60

Query: 61  TIDKSPV---SIGCFVGFDVLDPDSRHVVSIGKLKDIKFMSIFRFKVWWTTHWVGRNGGD 120
            +  S V   + G F+GFD      RHVV IGKL+D +FMSIFRFKVWWTTHWVG NG D
Sbjct: 61  LVPNSDVPAAAAGSFLGFDAPAAKDRHVVPIGKLRDTRFMSIFRFKVWWTTHWVGTNGRD 120

Query: 121 LESETQIVILEKSDS------GRPYVLLLPLVEGPFRSSIQPGE-DDFVDVCVESGSSKV 180
           +E+ETQ++IL++S +       RPYVLLLP+VEGPFR+ ++ G+ +D+V + +ESGSS V
Sbjct: 121 VENETQMMILDQSGTKSSPTGPRPYVLLLPIVEGPFRACLESGKAEDYVHMVLESGSSTV 180

Query: 181 VDSSFRSVLYLHA----------------------------------------------- 240
             S FRS +YLHA                                               
Sbjct: 181 RGSVFRSAVYLHAGDDPFDLVKDAMRVVRAHLGTFRLMEEKTPPPIVDKFGWCTWDAFYL 240

Query: 241 -----GIMEGVKHLVDGGCPPGLVLIDDGWQSIGHDADPITK--EGMNQTVAGEQMPCRL 300
                G+ EGV+ L DGGCPPGLVLIDDGWQSI HD D +    EGMN+T AGEQMPCRL
Sbjct: 241 KVHPEGVWEGVRRLADGGCPPGLVLIDDGWQSICHDDDDLGSGAEGMNRTSAGEQMPCRL 300

Query: 301 LKFQENYKFRDYVNPKSSGVGSGQKGMKAFIDELKGEYKTVEYVYVWHALCGYWGGLRPN 360
           +KFQENYKFR+Y           + GM  F+ E+K  + TVE VYVWHALCGYWGGLRP 
Sbjct: 301 IKFQENYKFREY-----------KGGMGGFVREMKAAFPTVEQVYVWHALCGYWGGLRPG 360

Query: 361 VPGLPEANVIQPVLSPGLQQTMEDLAVDKIVCHKVGIVPPEKAEEMYEGLHAHLENVGID 420
            PGLP A V+ P LSPGLQ+TMEDLAVDKIV + VG+V P +A E+YEGLH+HL+  GID
Sbjct: 361 APGLPPAKVVAPRLSPGLQRTMEDLAVDKIVNNGVGLVDPRRARELYEGLHSHLQASGID 420

Query: 421 GVKIDVIHLLEMLCEDYGGRVDLAKAYYKAMSKSIKKHFKGNGVIASMEHCNDFMFLGTE 480
           GVK+DVIHLLEM+CE+YGGRV+LAKAY+  +++S+++HF GNGVIASMEHCNDFM LGTE
Sbjct: 421 GVKVDVIHLLEMVCEEYGGRVELAKAYFAGLTESVRRHFNGNGVIASMEHCNDFMLLGTE 480

Query: 481 AISLGRVGDDFWCTDPSGDPNGTFWLQGCHMVHCAYNSLWMGNFIHPDWDMFQSTHPCAA 540
           A++LGRVGDDFWCTDPSGDP+GTFWLQGCHMVHCAYNSLWMG FIHPDWDMFQSTHPCAA
Sbjct: 481 AVALGRVGDDFWCTDPSGDPDGTFWLQGCHMVHCAYNSLWMGAFIHPDWDMFQSTHPCAA 540

Query: 541 FHAASRAISGGPIYVSDFVGKHNFELLRKLVLPDGSILRSEYYALPTRDCLFDDPLHDGE 600
           FHAASRA+SGGP+YVSD VG H+F+LLR+L LPDG+ILR E YALPTRDCLF DPLHDG+
Sbjct: 541 FHAASRAVSGGPVYVSDAVGCHDFDLLRRLALPDGTILRCERYALPTRDCLFADPLHDGK 600

Query: 601 TMLKIWNLNKFTGVIGAFNCQGGGWCRETRRNQCFSQYSKRVTSKTNAKDIEWNSGENPI 660
           TMLKIWN+NKF+GV+GAFNCQGGGW RE RRN C + +S  VT++ +  D+EW+ G    
Sbjct: 601 TMLKIWNVNKFSGVLGAFNCQGGGWSREARRNMCAAGFSVPVTARASPADVEWSHGGG-- 660

Query: 661 SIEGVKTFALYLYQAKKLILAKPTQNIDIALDPFDFELIFVSPVTTLI--ETAVQFAPIG 720
              G   FA+Y  +A+KL L +  +++++ L+PF +EL+ V+PV  ++  E  + FAPIG
Sbjct: 661 ---GGDRFAVYFVEARKLQLLRRDESVELTLEPFTYELLVVAPVRAIVSPELGIGFAPIG 720

Query: 721 LVNMLNIGGAIQ---SVYYDDDLSSVEIGVKGTGEMRVYASDKPRACRIDGEDVGFKYQD 730
           L NMLN GGA+Q   +   D D+++ E+ VKG GEM  Y+S +PR C+++G+D  FKY+D
Sbjct: 721 LANMLNAGGAVQGFEAARKDGDVAA-EVAVKGAGEMVAYSSARPRLCKVNGQDAEFKYED 780

BLAST of Carg15615-RA vs. Swiss-Prot
Match: sp|Q93XK2|STSYN_PEA (Stachyose synthase OS=Pisum sativum OX=3888 GN=STS1 PE=1 SV=1)

HSP 1 Score: 633.6 bits (1633), Expect = 2.6e-180
Identity = 346/865 (40.00%), Postives = 478/865 (55.26%), Query Frame = 0

Query: 1   MAPILKNGNSNIVVSFDGLNDLSSPFSIDGSNFTVNGHTFLSDVPENI-------VASPS 60
           MAP L +  SN++ +        S F +    F V G     DVPEN+       +  PS
Sbjct: 1   MAPPLNSTTSNLIKT-------ESIFDLSERKFKVKGFPLFHDVPENVSFRSFSSICKPS 60

Query: 61  ----PYTTIDK--SPVSIGCFVGFDVLDPDSRHVVSIGKLKDIKFMSIFRFKVWWTTHWV 120
               P + + K  +    G F GF    P  R + SIG      F+SIFRFK WW+T W+
Sbjct: 61  ESNAPPSLLQKVLAYSHKGGFFGFSHETPSDRLMNSIGSFNGKDFLSIFRFKTWWSTQWI 120

Query: 121 GRNGGDLESETQIVILEKSDSGRPYVLLLPLVEGPFRSSIQPGEDDFVDVCVESGSSKVV 180
           G++G DL+ ETQ +++E  ++ + YV+++P++E  FRS++ PG +D V +  ESGS+KV 
Sbjct: 121 GKSGSDLQMETQWILIEVPET-KSYVVIIPIIEKCFRSALFPGFNDHVKIIAESGSTKVK 180

Query: 181 DSSFRSVLYLH------------------------------------------------- 240
           +S+F S+ Y+H                                                 
Sbjct: 181 ESTFNSIAYVHFSENPYDLMKEAYSAIRVHLNSFRLLEEKTIPNLVDKFGWCTWDAFYLT 240

Query: 241 ---AGIMEGVKHLVDGGCPPGLVLIDDGWQSIGHDADPITKEGMNQTVAGEQMPCRLLKF 300
               GI  G+     GG  P  V+IDDGWQSI  D     ++  N  + GEQM  RL +F
Sbjct: 241 VNPIGIFHGLDDFSKGGVEPRFVIIDDGWQSISFDGYDPNEDAKNLVLGGEQMSGRLHRF 300

Query: 301 QENYKFRDY--------------------------------------VNPKSSGV----- 360
            E YKFR Y                                      ++ KSS +     
Sbjct: 301 DECYKFRKYESGLLLGPNSPPYDPNNFTDLILKGIEHEKLRKKREEAISSKSSDLAEIES 360

Query: 361 ------------------GSGQK-------GMKAFIDELKGEYKTVEYVYVWHALCGYWG 420
                              SG+K       G+KAF  +L+ ++K ++ VYVWHALCG WG
Sbjct: 361 KIKKVVKEIDDLFGGEQFSSGEKSEMKSEYGLKAFTKDLRTKFKGLDDVYVWHALCGAWG 420

Query: 421 GLRPNVPGLPEANVIQPVLSPGLQQTMEDLAVDKIVCHKVGIVPPEKAEEMYEGLHAHLE 480
           G+RP    L +  ++   LSPGL  TMEDLAV +I    +G+V P +A E+Y+ +H++L 
Sbjct: 421 GVRPETTHL-DTKIVPCKLSPGLDGTMEDLAVVEISKASLGLVHPSQANELYDSMHSYLA 480

Query: 481 NVGIDGVKIDVIHLLEMLCEDYGGRVDLAKAYYKAMSKSIKKHFKGNGVIASMEHCNDFM 540
             GI GVK+DVIH LE +C++YGGRVDLAK YY+ ++KSI K+F GNG+IASM+HCNDF 
Sbjct: 481 ESGITGVKVDVIHSLEYVCDEYGGRVDLAKVYYEGLTKSIVKNFNGNGMIASMQHCNDFF 540

Query: 541 FLGTEAISLGRVGDDFWCTDPSGDPNGTFWLQGCHMVHCAYNSLWMGNFIHPDWDMFQST 600
           FLGT+ IS+GRVGDDFW  DP+GDP G+FWLQG HM+HC+YNSLWMG  I PDWDMFQS 
Sbjct: 541 FLGTKQISMGRVGDDFWFQDPNGDPMGSFWLQGVHMIHCSYNSLWMGQMIQPDWDMFQSD 600

Query: 601 HPCAAFHAASRAISGGPIYVSDFVGKHNFELLRKLVLPDGSILRSEYYALPTRDCLFDDP 660
           H CA FHA SRAI GGPIYVSD VG H+F+L++KLV PDG+I +  Y+ LPTRDCLF +P
Sbjct: 601 HVCAKFHAGSRAICGGPIYVSDNVGSHDFDLIKKLVFPDGTIPKCIYFPLPTRDCLFKNP 660

Query: 661 LHDGETMLKIWNLNKFTGVIGAFNCQGGGWCRETRRNQCFSQYSKRVTSKTNAKDIEWNS 720
           L D  T+LKIWN NK+ GVIGAFNCQG GW    ++ + F +  K +    +  ++EW+ 
Sbjct: 661 LFDHTTVLKIWNFNKYGGVIGAFNCQGAGWDPIMQKFRGFPECYKPIPGTVHVTEVEWDQ 720

Query: 721 GENPISIEGVKTFALYLYQAKKL-ILAKPTQNIDIALDPFDFELIFVSPVTTLIETAVQF 730
            E    +   + + +YL QA++L ++   ++ I   + P  FEL    PVT L    ++F
Sbjct: 721 KEETSHLGKAEEYVVYLNQAEELSLMTLKSEPIQFTIQPSTFELYSFVPVTKLC-GGIKF 780

BLAST of Carg15615-RA vs. Swiss-Prot
Match: sp|Q9SYJ4|RFS4_ARATH (Probable galactinol--sucrose galactosyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=RFS4 PE=2 SV=3)

HSP 1 Score: 610.9 bits (1574), Expect = 1.8e-173
Identity = 331/841 (39.36%), Postives = 462/841 (54.93%), Query Frame = 0

Query: 29  DGSNFTVNGHTFLSDVPENIVASP-SPYTTIDKSPVSI----------GCFVGFDVLDPD 88
           +GS    +    L DVP+N+  +P S ++    +P+ I          G F+GF    P 
Sbjct: 38  EGSLCAKDSTPILFDVPQNVTFTPFSSHSISTDAPLPILLRVQANAHKGGFLGFTKESPS 97

Query: 89  SRHVVSIGKLKDIKFMSIFRFKVWWTTHWVGRNGGDLESETQIVILEKSDSGRPYVLLLP 148
            R   S+G+ +D +F+S+FRFK+WW+T W+G++G DL++ETQ V+L K      YV ++P
Sbjct: 98  DRLTNSLGRFEDREFLSLFRFKMWWSTAWIGKSGSDLQAETQWVML-KIPEIDSYVAIIP 157

Query: 149 LVEGPFRSSIQPGEDDFVDVCVESGSSKVVDSSFRSVLYLH------------------- 208
            +EG FR+S+ PGE   V +C ESGS+KV +SSF+S+ Y+H                   
Sbjct: 158 TIEGAFRASLTPGEKGNVLICAESGSTKVKESSFKSIAYIHICDNPYNLMKEAFSALRVH 217

Query: 209 ---------------------------------AGIMEGVKHLVDGGCPPGLVLIDDGWQ 268
                                            A I  GVK   DGG  P  V+IDDGWQ
Sbjct: 218 MNTFKLLEEKKLPKIVDKFGWCTWDACYLTVDPATIWTGVKEFEDGGVCPKFVIIDDGWQ 277

Query: 269 SIGHDADPITKEGMNQTVAGEQMPCRLLKFQENYKFRDY------------VNP------ 328
           SI  D D + K+  N  + GEQM  RL  F+E  KFR+Y             NP      
Sbjct: 278 SINFDGDELDKDAENLVLGGEQMTARLTSFKECKKFRNYKGGSFITSDASHFNPLKPKML 337

Query: 329 ------------------------------------------------KSSGVGSGQ--- 388
                                                           K   +GS     
Sbjct: 338 IYKATERIQAIILRRKLVKESGEQDLTELDEKIKILSEELNAMFDEVEKEESLGSDDVSG 397

Query: 389 KGMKAFIDELKGEYKTVEYVYVWHALCGYWGGLRPNVPGLPEANVIQPVLSPGLQQTMED 448
            GM AF  +L+  +K+++ +YVWHALCG W G+RP      +A V    LSP L  TM D
Sbjct: 398 SGMAAFTKDLRLRFKSLDDIYVWHALCGAWNGVRPETMMDLKAKVAPFELSPSLGATMAD 457

Query: 449 LAVDKIVCHKVGIVPPEKAEEMYEGLHAHLENVGIDGVKIDVIHLLEMLCEDYGGRVDLA 508
           LAVDK+V   +G+V P KA E Y+ +H++L +VG+ G KIDV   LE L E++GGRV+LA
Sbjct: 458 LAVDKVVEAGIGLVHPSKAHEFYDSMHSYLASVGVTGAKIDVFQTLESLAEEHGGRVELA 517

Query: 509 KAYYKAMSKSIKKHFKGNGVIASMEHCNDFMFLGTEAISLGRVGDDFWCTDPSGDPNGTF 568
           KAYY  +++S+ K+F G  VIASM+ CN+F FL T+ IS+GRVGDDFW  DP GDP G +
Sbjct: 518 KAYYDGLTESMIKNFNGTDVIASMQQCNEFFFLATKQISIGRVGDDFWWQDPYGDPQGVY 577

Query: 569 WLQGCHMVHCAYNSLWMGNFIHPDWDMFQSTHPCAAFHAASRAISGGPIYVSDFVGK--H 628
           WLQG HM+HC+YNS+WMG  I PDWDMFQS H CA +HAASRAI GGP+Y+SD +GK  H
Sbjct: 578 WLQGVHMIHCSYNSIWMGQMIQPDWDMFQSDHVCAEYHAASRAICGGPVYLSDHLGKASH 637

Query: 629 NFELLRKLVLPDGSILRSEYYALPTRDCLFDDPLHDGETMLKIWNLNKFTGVIGAFNCQG 688
           NF+L++KL   DG+I R  +YALPTRD LF +PL D E++LKI+N NKF GVIG FNCQG
Sbjct: 638 NFDLIKKLAFFDGTIPRCVHYALPTRDSLFKNPLFDKESILKIFNFNKFGGVIGTFNCQG 697

Query: 689 GGWCRETRRNQCFSQYSKRVTSKTNAKDIEWNSGENPISIEGVKT--FALYLYQAKK-LI 730
            GW  E  R + + +    V+   +  DIEW+        +   T  + +Y  Q+++ L 
Sbjct: 698 AGWSPEEHRFKGYKECYTTVSGTVHVSDIEWDQNPEAAGSQVTYTGDYLVYKQQSEEILF 757

BLAST of Carg15615-RA vs. TrEMBL
Match: tr|A0A1S3B929|A0A1S3B929_CUCME (probable galactinol--sucrose galactosyltransferase 5 OS=Cucumis melo OX=3656 GN=LOC103487113 PE=4 SV=1)

HSP 1 Score: 1382.9 bits (3578), Expect = 0.0e+00
Identity = 665/785 (84.71%), Postives = 700/785 (89.17%), Query Frame = 0

Query: 1   MAPILKNGNSNIVVSFDGLNDLSSPFSIDGSNFTVNGHTFLSDVPENIVASPSPYTTIDK 60
           MAP LKNG SN VVSFDGLND+SSPF+IDGS+FTV+GH FLSDVPENIVASPSPYT+IDK
Sbjct: 1   MAPSLKNGGSN-VVSFDGLNDMSSPFAIDGSDFTVDGHLFLSDVPENIVASPSPYTSIDK 60

Query: 61  SPVSIGCFVGFDVLDPDSRHVVSIGKLKDIKFMSIFRFKVWWTTHWVGRNGGDLESETQI 120
           SPVS+GCFVGFD  +PDSRHVVSIGKLKDI+FMSIFRFKVWWTTHWVGRNGGDLESETQI
Sbjct: 61  SPVSVGCFVGFDASEPDSRHVVSIGKLKDIRFMSIFRFKVWWTTHWVGRNGGDLESETQI 120

Query: 121 VILEKSDSGRPYVLLLPLVEGPFRSSIQPGEDDFVDVCVESGSSKVVDSSFRSVLYLHA- 180
           VILEKSDSGRPYVLLLP+VEGPFR+SIQPG+DDFVDVCVESGSSKVVD+SFRSVLYLHA 
Sbjct: 121 VILEKSDSGRPYVLLLPIVEGPFRTSIQPGDDDFVDVCVESGSSKVVDASFRSVLYLHAG 180

Query: 181 ---------------------------------------------------GIMEGVKHL 240
                                                              G++EGVKHL
Sbjct: 181 DDPFALVKEAMKIVRTHLGTFRLLEEKTPPGIVDKFGWCTWDAFYLTVHPQGVIEGVKHL 240

Query: 241 VDGGCPPGLVLIDDGWQSIGHDADPITKEGMNQTVAGEQMPCRLLKFQENYKFRDYVNPK 300
           VDGGCPPGLVLIDDGWQSIGHD+DPITKEGMNQTVAGEQMPCRLLKFQENYKFRDYVNPK
Sbjct: 241 VDGGCPPGLVLIDDGWQSIGHDSDPITKEGMNQTVAGEQMPCRLLKFQENYKFRDYVNPK 300

Query: 301 SSGVGSGQKGMKAFIDELKGEYKTVEYVYVWHALCGYWGGLRPNVPGLPEANVIQPVLSP 360
           ++G G GQKGMKAFIDELKGE+KTVEYVYVWHALCGYWGGLRP VPGLPEA VIQPVLSP
Sbjct: 301 ATGSGDGQKGMKAFIDELKGEFKTVEYVYVWHALCGYWGGLRPQVPGLPEARVIQPVLSP 360

Query: 361 GLQQTMEDLAVDKIVCHKVGIVPPEKAEEMYEGLHAHLENVGIDGVKIDVIHLLEMLCED 420
           GLQ TMEDLAVDKIV HKVG+VPPEKAEEMYEGLH+HLE VGIDGVKIDVIHLLEMLCED
Sbjct: 361 GLQMTMEDLAVDKIVLHKVGLVPPEKAEEMYEGLHSHLEKVGIDGVKIDVIHLLEMLCED 420

Query: 421 YGGRVDLAKAYYKAMSKSIKKHFKGNGVIASMEHCNDFMFLGTEAISLGRVGDDFWCTDP 480
           YGGRVDLAKAYYKAM+KSI KHFKGNGVIASMEHCNDFMFLGTEAISLGRVGDDFWCTDP
Sbjct: 421 YGGRVDLAKAYYKAMTKSINKHFKGNGVIASMEHCNDFMFLGTEAISLGRVGDDFWCTDP 480

Query: 481 SGDPNGTFWLQGCHMVHCAYNSLWMGNFIHPDWDMFQSTHPCAAFHAASRAISGGPIYVS 540
           SGDPNGTFWLQGCHMVHCAYNSLWMGNFIHPDWDMFQSTHPCAAFHAASRAISGGPIYVS
Sbjct: 481 SGDPNGTFWLQGCHMVHCAYNSLWMGNFIHPDWDMFQSTHPCAAFHAASRAISGGPIYVS 540

Query: 541 DFVGKHNFELLRKLVLPDGSILRSEYYALPTRDCLFDDPLHDGETMLKIWNLNKFTGVIG 600
           D VGKHNF+LL+KLVLPDGSILRSEYYALPTRDCLF DPLH+GETMLKIWNLNKFTGVIG
Sbjct: 541 DSVGKHNFDLLKKLVLPDGSILRSEYYALPTRDCLFADPLHNGETMLKIWNLNKFTGVIG 600

Query: 601 AFNCQGGGWCRETRRNQCFSQYSKRVTSKTNAKDIEWNSGENPISIEGVKTFALYLYQAK 660
           AFNCQGGGWCRETRRNQCFSQYSKRVTSKTN KDIEW+SGENPISIEGVKTF LYLYQAK
Sbjct: 601 AFNCQGGGWCRETRRNQCFSQYSKRVTSKTNPKDIEWHSGENPISIEGVKTFVLYLYQAK 660

Query: 661 KLILAKPTQNIDIALDPFDFELIFVSPVTTLIETAVQFAPIGLVNMLNIGGAIQSVYYDD 720
           KLI++KP+Q++DIALDPF+FELI VSPVTTL +T++ FAPIGLVNMLN GGAIQSV YDD
Sbjct: 661 KLIISKPSQDLDIALDPFEFELITVSPVTTLTQTSLHFAPIGLVNMLNTGGAIQSVDYDD 720

Query: 721 DLSSVEIGVKGTGEMRVYASDKPRACRIDGEDVGFKY-QDQMVAVQVPWP---GSSGIST 730
           DLSSVEIGVKG GEMRV+AS KPRACRIDGEDVGFKY QDQMV VQVPWP    S GIS 
Sbjct: 721 DLSSVEIGVKGCGEMRVFASKKPRACRIDGEDVGFKYDQDQMVVVQVPWPVDSSSGGISV 780

BLAST of Carg15615-RA vs. TrEMBL
Match: tr|B5G4T9|B5G4T9_CUCSA (Raffinose synthase OS=Cucumis sativus OX=3659 GN=Csa_3G838720 PE=2 SV=1)

HSP 1 Score: 1379.4 bits (3569), Expect = 0.0e+00
Identity = 663/785 (84.46%), Postives = 701/785 (89.30%), Query Frame = 0

Query: 1   MAPILKNGNSNIVVSFDGLNDLSSPFSIDGSNFTVNGHTFLSDVPENIVASPSPYTTIDK 60
           MAP  KNG SN VVSFDGLND+SSPF+IDGS+FTVNGH+FLSDVPENIVASPSPYT+IDK
Sbjct: 1   MAPSFKNGGSN-VVSFDGLNDMSSPFAIDGSDFTVNGHSFLSDVPENIVASPSPYTSIDK 60

Query: 61  SPVSIGCFVGFDVLDPDSRHVVSIGKLKDIKFMSIFRFKVWWTTHWVGRNGGDLESETQI 120
           SPVS+GCFVGFD  +PDSRHVVSIGKLKDI+FMSIFRFKVWWTTHWVGRNGGDLESETQI
Sbjct: 61  SPVSVGCFVGFDASEPDSRHVVSIGKLKDIRFMSIFRFKVWWTTHWVGRNGGDLESETQI 120

Query: 121 VILEKSDSGRPYVLLLPLVEGPFRSSIQPGEDDFVDVCVESGSSKVVDSSFRSVLYLHA- 180
           VILEKSDSGRPYVLLLP+VEGPFR+SIQPG+DDFVDVCVESGSSKVVD+SFRS+LYLHA 
Sbjct: 121 VILEKSDSGRPYVLLLPIVEGPFRTSIQPGDDDFVDVCVESGSSKVVDASFRSMLYLHAG 180

Query: 181 ---------------------------------------------------GIMEGVKHL 240
                                                              G++EGV+HL
Sbjct: 181 DDPFALVKEAMKIVRTHLGTFRLLEEKTPPGIVDKFGWCTWDAFYLTVHPQGVIEGVRHL 240

Query: 241 VDGGCPPGLVLIDDGWQSIGHDADPITKEGMNQTVAGEQMPCRLLKFQENYKFRDYVNPK 300
           VDGGCPPGLVLIDDGWQSIGHD+DPITKEGMNQTVAGEQMPCRLLKFQENYKFRDYVNPK
Sbjct: 241 VDGGCPPGLVLIDDGWQSIGHDSDPITKEGMNQTVAGEQMPCRLLKFQENYKFRDYVNPK 300

Query: 301 SSGVGSGQKGMKAFIDELKGEYKTVEYVYVWHALCGYWGGLRPNVPGLPEANVIQPVLSP 360
           ++G  +GQKGMKAFIDELKGE+KTVE+VYVWHALCGYWGGLRP VPGLPEA VIQPVLSP
Sbjct: 301 ATGPRAGQKGMKAFIDELKGEFKTVEHVYVWHALCGYWGGLRPQVPGLPEARVIQPVLSP 360

Query: 361 GLQQTMEDLAVDKIVCHKVGIVPPEKAEEMYEGLHAHLENVGIDGVKIDVIHLLEMLCED 420
           GLQ TMEDLAVDKIV HKVG+VPPEKAEEMYEGLHAHLE VGIDGVKIDVIHLLEMLCED
Sbjct: 361 GLQMTMEDLAVDKIVLHKVGLVPPEKAEEMYEGLHAHLEKVGIDGVKIDVIHLLEMLCED 420

Query: 421 YGGRVDLAKAYYKAMSKSIKKHFKGNGVIASMEHCNDFMFLGTEAISLGRVGDDFWCTDP 480
           YGGRVDLAKAYYKAM+KSI KHFKGNGVIASMEHCNDFMFLGTEAISLGRVGDDFWCTDP
Sbjct: 421 YGGRVDLAKAYYKAMTKSINKHFKGNGVIASMEHCNDFMFLGTEAISLGRVGDDFWCTDP 480

Query: 481 SGDPNGTFWLQGCHMVHCAYNSLWMGNFIHPDWDMFQSTHPCAAFHAASRAISGGPIYVS 540
           SGDPNGTFWLQGCHMVHCAYNSLWMGNFIHPDWDMFQSTHPCAAFHAASRAISGGPIYVS
Sbjct: 481 SGDPNGTFWLQGCHMVHCAYNSLWMGNFIHPDWDMFQSTHPCAAFHAASRAISGGPIYVS 540

Query: 541 DFVGKHNFELLRKLVLPDGSILRSEYYALPTRDCLFDDPLHDGETMLKIWNLNKFTGVIG 600
           D VGKHNF+LL+KLVLPDGSILRSEYYALPTRDCLF+DPLH+GETMLKIWNLNKFTGVIG
Sbjct: 541 DSVGKHNFDLLKKLVLPDGSILRSEYYALPTRDCLFEDPLHNGETMLKIWNLNKFTGVIG 600

Query: 601 AFNCQGGGWCRETRRNQCFSQYSKRVTSKTNAKDIEWNSGENPISIEGVKTFALYLYQAK 660
           AFNCQGGGWCRETRRNQCFSQYSKRVTSKTN KDIEW+SGENPISIEGVKTFALYLYQAK
Sbjct: 601 AFNCQGGGWCRETRRNQCFSQYSKRVTSKTNPKDIEWHSGENPISIEGVKTFALYLYQAK 660

Query: 661 KLILAKPTQNIDIALDPFDFELIFVSPVTTLIETAVQFAPIGLVNMLNIGGAIQSVYYDD 720
           KLIL+KP+Q++DIALDPF+FELI VSPVT LI+T++ FAPIGLVNMLN  GAIQSV YDD
Sbjct: 661 KLILSKPSQDLDIALDPFEFELITVSPVTKLIQTSLHFAPIGLVNMLNTSGAIQSVDYDD 720

Query: 721 DLSSVEIGVKGTGEMRVYASDKPRACRIDGEDVGFKY-QDQMVAVQVPWP---GSSGIST 730
           DLSSVEIGVKG GEMRV+AS KPRACRIDGEDVGFKY QDQMV VQVPWP    S GIS 
Sbjct: 721 DLSSVEIGVKGCGEMRVFASKKPRACRIDGEDVGFKYDQDQMVVVQVPWPIDSSSGGISV 780

BLAST of Carg15615-RA vs. TrEMBL
Match: tr|Q9ZT62|Q9ZT62_CUCSA (Raffinose synthase OS=Cucumis sativus OX=3659 GN=Rfs PE=2 SV=1)

HSP 1 Score: 1372.5 bits (3551), Expect = 0.0e+00
Identity = 660/785 (84.08%), Postives = 699/785 (89.04%), Query Frame = 0

Query: 1   MAPILKNGNSNIVVSFDGLNDLSSPFSIDGSNFTVNGHTFLSDVPENIVASPSPYTTIDK 60
           MAP  KNG SN VVSFDGLND+SSPF+IDGS+FTVNGH+FLSDVPENIVASPSPYT+IDK
Sbjct: 1   MAPSFKNGGSN-VVSFDGLNDMSSPFAIDGSDFTVNGHSFLSDVPENIVASPSPYTSIDK 60

Query: 61  SPVSIGCFVGFDVLDPDSRHVVSIGKLKDIKFMSIFRFKVWWTTHWVGRNGGDLESETQI 120
           SPVS+GCFVGFD  +PDSRHVVSIGKLKDI+FMSIFRFKVWWTTHWVGRNGGDLESETQI
Sbjct: 61  SPVSVGCFVGFDASEPDSRHVVSIGKLKDIRFMSIFRFKVWWTTHWVGRNGGDLESETQI 120

Query: 121 VILEKSDSGRPYVLLLPLVEGPFRSSIQPGEDDFVDVCVESGSSKVVDSSFRSVLYLHA- 180
           VILEKSDSGRPYV LLP+VEGPFR+SIQPG+DDFVDVCVESGSSKVVD+SFRS+LYLHA 
Sbjct: 121 VILEKSDSGRPYVFLLPIVEGPFRTSIQPGDDDFVDVCVESGSSKVVDASFRSMLYLHAG 180

Query: 181 ---------------------------------------------------GIMEGVKHL 240
                                                              G++EGV+HL
Sbjct: 181 DDPFALVKEAMKIVRTHLGTFRLLEEKTPPGIVDKFGWCTWDAFYLTVHPQGVIEGVRHL 240

Query: 241 VDGGCPPGLVLIDDGWQSIGHDADPITKEGMNQTVAGEQMPCRLLKFQENYKFRDYVNPK 300
           VDGGCPPGLVLIDDGWQSIGHD+DPITKEGMNQTVAGEQMPCRLLKFQENYKFRDYVNPK
Sbjct: 241 VDGGCPPGLVLIDDGWQSIGHDSDPITKEGMNQTVAGEQMPCRLLKFQENYKFRDYVNPK 300

Query: 301 SSGVGSGQKGMKAFIDELKGEYKTVEYVYVWHALCGYWGGLRPNVPGLPEANVIQPVLSP 360
           ++G  +GQKGMKAFIDELKGE+KTVE+VYVWHALCGYWGGLRP VPGLPEA VIQPVLSP
Sbjct: 301 ATGPRAGQKGMKAFIDELKGEFKTVEHVYVWHALCGYWGGLRPQVPGLPEARVIQPVLSP 360

Query: 361 GLQQTMEDLAVDKIVCHKVGIVPPEKAEEMYEGLHAHLENVGIDGVKIDVIHLLEMLCED 420
           GLQ TMEDLAVDKIV HKVG+VPPEKAEEMYEGLHAHLE VGIDGVKIDVIHLLEMLCED
Sbjct: 361 GLQMTMEDLAVDKIVLHKVGLVPPEKAEEMYEGLHAHLEKVGIDGVKIDVIHLLEMLCED 420

Query: 421 YGGRVDLAKAYYKAMSKSIKKHFKGNGVIASMEHCNDFMFLGTEAISLGRVGDDFWCTDP 480
           YGGRVDLAKAYYKAM+KSI KHFKGNGVIASMEHCNDFMFLGTEAISLGRVGDDFWCTDP
Sbjct: 421 YGGRVDLAKAYYKAMTKSINKHFKGNGVIASMEHCNDFMFLGTEAISLGRVGDDFWCTDP 480

Query: 481 SGDPNGTFWLQGCHMVHCAYNSLWMGNFIHPDWDMFQSTHPCAAFHAASRAISGGPIYVS 540
           SGDPNGTFWLQGCHMVHCA +SLWMGNFIHPDWDMFQSTHPCAAFHAASRAISGGPIYVS
Sbjct: 481 SGDPNGTFWLQGCHMVHCANDSLWMGNFIHPDWDMFQSTHPCAAFHAASRAISGGPIYVS 540

Query: 541 DFVGKHNFELLRKLVLPDGSILRSEYYALPTRDCLFDDPLHDGETMLKIWNLNKFTGVIG 600
           D VGKHNF+LL+KLVLPDGSILRSEYYALPTRDCLF+DPLH+GETMLKIWNLNKFTGVIG
Sbjct: 541 DSVGKHNFDLLKKLVLPDGSILRSEYYALPTRDCLFEDPLHNGETMLKIWNLNKFTGVIG 600

Query: 601 AFNCQGGGWCRETRRNQCFSQYSKRVTSKTNAKDIEWNSGENPISIEGVKTFALYLYQAK 660
           AFNCQGGGWCRETRRNQCFSQYSKRVTSKTN KDIEW+SGENPISIEGVKTFALYLYQAK
Sbjct: 601 AFNCQGGGWCRETRRNQCFSQYSKRVTSKTNPKDIEWHSGENPISIEGVKTFALYLYQAK 660

Query: 661 KLILAKPTQNIDIALDPFDFELIFVSPVTTLIETAVQFAPIGLVNMLNIGGAIQSVYYDD 720
           KLIL+KP+Q++DIALDPF+FELI VSPVT LI+T++ FAPIGLVNMLN  GAIQSV YDD
Sbjct: 661 KLILSKPSQDLDIALDPFEFELITVSPVTKLIQTSLHFAPIGLVNMLNTSGAIQSVDYDD 720

Query: 721 DLSSVEIGVKGTGEMRVYASDKPRACRIDGEDVGFKY-QDQMVAVQVPWP---GSSGIST 730
           DLSSVEIGVKG GEMRV+AS KPRACRIDGEDVGFKY QDQMV VQVPWP    S GIS 
Sbjct: 721 DLSSVEIGVKGCGEMRVFASKKPRACRIDGEDVGFKYDQDQMVVVQVPWPIDSSSGGISV 780

BLAST of Carg15615-RA vs. TrEMBL
Match: tr|A0A061EBW1|A0A061EBW1_THECC (Raffinose synthase family protein OS=Theobroma cacao OX=3641 GN=TCM_016942 PE=4 SV=1)

HSP 1 Score: 1196.4 bits (3094), Expect = 0.0e+00
Identity = 563/783 (71.90%), Postives = 643/783 (82.12%), Query Frame = 0

Query: 1   MAPILKNGNSNIVVSFDGLNDLSSPFSIDGSNFTVNGHTFLSDVPENIVASPSPY--TTI 60
           MAP L   +S +    D  +   SPF+++GSNF  NGH FLSDVP+NI  +PSPY  +T 
Sbjct: 1   MAPSLSKASSGVSGLVDSHH--QSPFALEGSNFIANGHVFLSDVPDNITVTPSPYGSSTT 60

Query: 61  DKSPVSIGCFVGFDVLDPDSRHVVSIGKLKDIKFMSIFRFKVWWTTHWVGRNGGDLESET 120
           DKS  ++G FVGFD ++P SRHVV IGKLK+IKFMSIFRFKVWWTTHWVG NGGDLE+ET
Sbjct: 61  DKSKSTVGSFVGFDAVEPASRHVVPIGKLKNIKFMSIFRFKVWWTTHWVGSNGGDLENET 120

Query: 121 QIVILEKSDSGRPYVLLLPLVEGPFRSSIQPGEDDFVDVCVESGSSKVVDSSFRSVLYLH 180
           Q+VIL+KSDSGRPYVLLLPL+EG FR+S+QPG DD VD+CVESGS+KV  + FRSVLY+H
Sbjct: 121 QMVILDKSDSGRPYVLLLPLLEGSFRASLQPGTDDNVDICVESGSTKVTSAGFRSVLYVH 180

Query: 181 A----------------------------------------------------GIMEGVK 240
           A                                                    G+ EGVK
Sbjct: 181 AGEDPFNLVKEAMKVIRCHLGTFKLLEEKTPPGIVDKFGWCTWDAFYLTVHPQGVWEGVK 240

Query: 241 HLVDGGCPPGLVLIDDGWQSIGHDADPITKEGMNQTVAGEQMPCRLLKFQENYKFRDYVN 300
            LVDGGCPPGLVLIDDGWQSI HD DPITKEGMN TVAGEQMPCRLLKFQENYKFRDYV+
Sbjct: 241 GLVDGGCPPGLVLIDDGWQSISHDEDPITKEGMNCTVAGEQMPCRLLKFQENYKFRDYVS 300

Query: 301 PKSSGVGSGQKGMKAFIDELKGEYKTVEYVYVWHALCGYWGGLRPNVPGLPEANVIQPVL 360
           PK+SG G+  KGM AFI +LK ++ TV++VYVWHALCGYWGGLRPNVPGLPE  V+QP L
Sbjct: 301 PKTSGTGAPNKGMGAFIKDLKEQFNTVDFVYVWHALCGYWGGLRPNVPGLPETKVVQPEL 360

Query: 361 SPGLQQTMEDLAVDKIVCHKVGIVPPEKAEEMYEGLHAHLENVGIDGVKIDVIHLLEMLC 420
           SPG ++TMEDLAVDKIV   VG+VPPE  +++YEG+H+HLE VGIDGVK+DVIHLLEMLC
Sbjct: 361 SPGAKKTMEDLAVDKIVSTGVGLVPPEMVDQLYEGIHSHLEKVGIDGVKVDVIHLLEMLC 420

Query: 421 EDYGGRVDLAKAYYKAMSKSIKKHFKGNGVIASMEHCNDFMFLGTEAISLGRVGDDFWCT 480
           E+YGGRV+LAKAYY+A++ S++KHFKGNGVIASMEHCNDFMFLGTEAI LGRVGDDFWCT
Sbjct: 421 ENYGGRVELAKAYYRALTDSVRKHFKGNGVIASMEHCNDFMFLGTEAICLGRVGDDFWCT 480

Query: 481 DPSGDPNGTFWLQGCHMVHCAYNSLWMGNFIHPDWDMFQSTHPCAAFHAASRAISGGPIY 540
           DPSGDPNGTFWLQGCHMVHCAYNSLWMGNFIHPDWDMFQSTHPCA FHAASRAISGGPIY
Sbjct: 481 DPSGDPNGTFWLQGCHMVHCAYNSLWMGNFIHPDWDMFQSTHPCAEFHAASRAISGGPIY 540

Query: 541 VSDFVGKHNFELLRKLVLPDGSILRSEYYALPTRDCLFDDPLHDGETMLKIWNLNKFTGV 600
           VSD VGKHNF LL++LVLPDGSILR +YYALPTRDCLF+DPLHDG+TMLKIWNLNK+TGV
Sbjct: 541 VSDTVGKHNFPLLKRLVLPDGSILRCQYYALPTRDCLFEDPLHDGKTMLKIWNLNKYTGV 600

Query: 601 IGAFNCQGGGWCRETRRNQCFSQYSKRVTSKTNAKDIEWNSGENPISIEGVKTFALYLYQ 660
           IGAFNCQGGGWCRETRRNQC SQ+S  VT+KTN KDIEW SG+NPISIE V+ FALYL Q
Sbjct: 601 IGAFNCQGGGWCRETRRNQCASQFSNMVTAKTNPKDIEWKSGKNPISIEAVQVFALYLSQ 660

Query: 661 AKKLILAKPTQNIDIALDPFDFELIFVSPVTTLIETAVQFAPIGLVNMLNIGGAIQSVYY 720
           +KKL+L+KP ++I+I+L+PF+FELI VSPVT L   +V FAPIGLVNMLN GGAIQS+ Y
Sbjct: 661 SKKLVLSKPAESIEISLEPFNFELITVSPVTVLAGKSVHFAPIGLVNMLNAGGAIQSLAY 720

Query: 721 DDDLSSVEIGVKGTGEMRVYASDKPRACRIDGEDVGFKYQDQMVAVQVPWPGSSGISTIE 730
           D+  SSVEIGVKG GEMRV+ASDKPRAC+IDG+D+GF+Y+ QMV VQVPW   +G+STIE
Sbjct: 721 DEFESSVEIGVKGAGEMRVFASDKPRACKIDGKDIGFEYEGQMVIVQVPWSSPAGLSTIE 780

BLAST of Carg15615-RA vs. TrEMBL
Match: tr|A0A1R3GMA8|A0A1R3GMA8_COCAP (Raffinose synthase OS=Corchorus capsularis OX=210143 GN=CCACVL1_24973 PE=4 SV=1)

HSP 1 Score: 1185.6 bits (3066), Expect = 0.0e+00
Identity = 558/784 (71.17%), Postives = 638/784 (81.38%), Query Frame = 0

Query: 1   MAPILKNGNSNIVVSFDGLNDLSSPFSIDGSNFTVNGHTFLSDVPENIVASPSPY---TT 60
           MAP L   NS +    DG     SP S++GSNFT NGH FLSDVP+NI  +PSPY   ++
Sbjct: 1   MAPSLSKANSGVSGVADG---HKSPISLEGSNFTANGHVFLSDVPDNITITPSPYGGSSS 60

Query: 61  IDKSPVSIGCFVGFDVLDPDSRHVVSIGKLKDIKFMSIFRFKVWWTTHWVGRNGGDLESE 120
            DKS  ++G FVGFD +  DSRHVV IGKLK+IKFMSIFRFKVWWTTHWVG NGGDLE+E
Sbjct: 61  PDKSITTVGSFVGFDAVKADSRHVVPIGKLKNIKFMSIFRFKVWWTTHWVGSNGGDLENE 120

Query: 121 TQIVILEKSDSGRPYVLLLPLVEGPFRSSIQPGEDDFVDVCVESGSSKVVDSSFRSVLYL 180
           TQ+VIL++SDSGRPYVLLLPL+EGPFR+S+QPG DD VD+CVESGS+KV  + FRSVLY 
Sbjct: 121 TQMVILDRSDSGRPYVLLLPLIEGPFRASLQPGTDDNVDICVESGSTKVTSAGFRSVLYF 180

Query: 181 HA----------------------------------------------------GIMEGV 240
           H                                                     G+ EGV
Sbjct: 181 HVGEDPFKLVKEAMKVMRVHLGTFKLLEEKTPPGIVDKFGWCTWDAFYLTVHPQGVWEGV 240

Query: 241 KHLVDGGCPPGLVLIDDGWQSIGHDADPITKEGMNQTVAGEQMPCRLLKFQENYKFRDYV 300
           K LVDGGCPPGLVLIDDGWQSIGHDADPITKEGMN TVAGEQMPCRLLKFQENYKFRDYV
Sbjct: 241 KGLVDGGCPPGLVLIDDGWQSIGHDADPITKEGMNCTVAGEQMPCRLLKFQENYKFRDYV 300

Query: 301 NPKSSGVGSGQKGMKAFIDELKGEYKTVEYVYVWHALCGYWGGLRPNVPGLPEANVIQPV 360
           +PKSSG G+  KGM AF+ +LK E+ TV++VYVWHALCGYWGGLRPNVPGLPE  VI+P 
Sbjct: 301 SPKSSGSGAPNKGMGAFVKDLKEEFSTVDFVYVWHALCGYWGGLRPNVPGLPENKVIKPE 360

Query: 361 LSPGLQQTMEDLAVDKIVCHKVGIVPPEKAEEMYEGLHAHLENVGIDGVKIDVIHLLEML 420
           LSPGLQ+TMEDLAVDKIV   +G+VPPE A+++YEGLH+HLE VGIDGVK+DVIHLLEML
Sbjct: 361 LSPGLQKTMEDLAVDKIVNTGIGLVPPEMADQLYEGLHSHLEKVGIDGVKVDVIHLLEML 420

Query: 421 CEDYGGRVDLAKAYYKAMSKSIKKHFKGNGVIASMEHCNDFMFLGTEAISLGRVGDDFWC 480
           CE+YGGRVDLAKAYY+A++ S+K+HFKGNGVIASMEHCNDFMFLGTEAI LGRVGDDFWC
Sbjct: 421 CENYGGRVDLAKAYYRALTDSVKRHFKGNGVIASMEHCNDFMFLGTEAICLGRVGDDFWC 480

Query: 481 TDPSGDPNGTFWLQGCHMVHCAYNSLWMGNFIHPDWDMFQSTHPCAAFHAASRAISGGPI 540
           TDPSGDPNGTFWLQGCHMVHCAYNSLWMGNFIHPDWDMFQSTHPCA +HAASRAISGGPI
Sbjct: 481 TDPSGDPNGTFWLQGCHMVHCAYNSLWMGNFIHPDWDMFQSTHPCAEYHAASRAISGGPI 540

Query: 541 YVSDFVGKHNFELLRKLVLPDGSILRSEYYALPTRDCLFDDPLHDGETMLKIWNLNKFTG 600
           YVSD VGKHNF LL++LV+PDGSILR EYYALPTRDCLF+DPLHDG+TMLKIWNLNK+TG
Sbjct: 541 YVSDTVGKHNFPLLKRLVMPDGSILRCEYYALPTRDCLFEDPLHDGKTMLKIWNLNKYTG 600

Query: 601 VIGAFNCQGGGWCRETRRNQCFSQYSKRVTSKTNAKDIEWNSGENPISIEGVKTFALYLY 660
           VIG FNCQGGGWCRETRRNQCFSQ+S  VT+KTNA DIEWNSG+NPI IEG + FA+Y  
Sbjct: 601 VIGVFNCQGGGWCRETRRNQCFSQFSHMVTTKTNAMDIEWNSGKNPIPIEGGQVFAMYFS 660

Query: 661 QAKKLILAKPTQNIDIALDPFDFELIFVSPVTTLIETAVQFAPIGLVNMLNIGGAIQSVY 720
           Q+KKL+L+ PT+ I+I+L+PF+FELI V+PVT L   +V FAPIGLVNMLN+GGAIQS+ 
Sbjct: 661 QSKKLLLSNPTEKIEISLEPFNFELITVAPVTVLAGKSVHFAPIGLVNMLNVGGAIQSLA 720

Query: 721 YDDDLSSVEIGVKGTGEMRVYASDKPRACRIDGEDVGFKYQDQMVAVQVPWPGSSGISTI 730
           YD+  S  ++ VKG+GEMRV+AS KPR C+IDG++V F+Y+  MV VQVPW   SGIS++
Sbjct: 721 YDELQSCAKVEVKGSGEMRVFASAKPRICKIDGKEVNFEYEGHMVIVQVPWSSPSGISSV 780

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022928549.10.0e+0093.21probable galactinol--sucrose galactosyltransferase 5 [Cucurbita moschata][more]
XP_022989256.10.0e+0091.93probable galactinol--sucrose galactosyltransferase 5 [Cucurbita maxima][more]
XP_023529233.10.0e+0091.81probable galactinol--sucrose galactosyltransferase 5 [Cucurbita pepo subsp. pepo... [more]
XP_023526448.10.0e+0085.28probable galactinol--sucrose galactosyltransferase 5 [Cucurbita pepo subsp. pepo... [more]
XP_022934881.10.0e+0084.76probable galactinol--sucrose galactosyltransferase 5 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT5G40390.14.8e-30263.81Raffinose synthase family protein[more]
AT4G01970.11.0e-17439.36stachyose synthase[more]
AT3G57520.17.6e-13834.73seed imbibition 2[more]
AT5G20250.45.4e-13636.03Raffinose synthase family protein[more]
AT1G55740.11.1e-13335.29seed imbibition 1[more]
Match NameE-valueIdentityDescription
sp|Q9FND9|RFS5_ARATH8.7e-30163.81Probable galactinol--sucrose galactosyltransferase 5 OS=Arabidopsis thaliana OX=... [more]
sp|Q8VWN6|RFS_PEA2.1e-28659.35Galactinol--sucrose galactosyltransferase OS=Pisum sativum OX=3888 GN=RFS PE=1 S... [more]
sp|Q5VQG4|RFS_ORYSJ1.5e-26856.91Galactinol--sucrose galactosyltransferase OS=Oryza sativa subsp. japonica OX=399... [more]
sp|Q93XK2|STSYN_PEA2.6e-18040.00Stachyose synthase OS=Pisum sativum OX=3888 GN=STS1 PE=1 SV=1[more]
sp|Q9SYJ4|RFS4_ARATH1.8e-17339.36Probable galactinol--sucrose galactosyltransferase 4 OS=Arabidopsis thaliana OX=... [more]
Match NameE-valueIdentityDescription
tr|A0A1S3B929|A0A1S3B929_CUCME0.0e+0084.71probable galactinol--sucrose galactosyltransferase 5 OS=Cucumis melo OX=3656 GN=... [more]
tr|B5G4T9|B5G4T9_CUCSA0.0e+0084.46Raffinose synthase OS=Cucumis sativus OX=3659 GN=Csa_3G838720 PE=2 SV=1[more]
tr|Q9ZT62|Q9ZT62_CUCSA0.0e+0084.08Raffinose synthase OS=Cucumis sativus OX=3659 GN=Rfs PE=2 SV=1[more]
tr|A0A061EBW1|A0A061EBW1_THECC0.0e+0071.90Raffinose synthase family protein OS=Theobroma cacao OX=3641 GN=TCM_016942 PE=4 ... [more]
tr|A0A1R3GMA8|A0A1R3GMA8_COCAP0.0e+0071.17Raffinose synthase OS=Corchorus capsularis OX=210143 GN=CCACVL1_24973 PE=4 SV=1[more]
The following terms have been associated with this mRNA:
Vocabulary: INTERPRO
TermDefinition
IPR017853Glycoside_hydrolase_SF
IPR008811Glycosyl_hydrolases_36
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0003674 molecular_function

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Carg15615Carg15615gene


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Carg15615-RACarg15615-RA-proteinpolypeptide


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg15615-RA.CDS.5Carg15615-RA.CDS.5CDS
Carg15615-RA.CDS.4Carg15615-RA.CDS.4CDS
Carg15615-RA.CDS.3Carg15615-RA.CDS.3CDS
Carg15615-RA.CDS.2Carg15615-RA.CDS.2CDS
Carg15615-RA.CDS.1Carg15615-RA.CDS.1CDS


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg15615-RA.exon.5Carg15615-RA.exon.5exon
Carg15615-RA.exon.4Carg15615-RA.exon.4exon
Carg15615-RA.exon.3Carg15615-RA.exon.3exon
Carg15615-RA.exon.2Carg15615-RA.exon.2exon
Carg15615-RA.exon.1Carg15615-RA.exon.1exon


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008811Glycosyl hydrolases 36PFAMPF05691Raffinose_syncoord: 180..713
e-value: 2.7E-249
score: 828.8
IPR008811Glycosyl hydrolases 36PANTHERPTHR31268FAMILY NOT NAMEDcoord: 180..729
IPR008811Glycosyl hydrolases 36PANTHERPTHR31268FAMILY NOT NAMEDcoord: 1..180
NoneNo IPR availablePANTHERPTHR31268:SF2GALACTINOL--SUCROSE GALACTOSYLTRANSFERASE 5-RELATEDcoord: 1..180
coord: 180..729
IPR017853Glycoside hydrolase superfamilySUPERFAMILYSSF51445(Trans)glycosidasescoord: 179..209
coord: 250..287
coord: 327..501