Carg14818-RA (mRNA) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg14818-RA
Type	mRNA
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	glyoxylate/hydroxypyruvate reductase HPR3-like
Location	Cucurbita_argyrosperma_scaffold_079 : 117546 .. 117953 (-)
Sequence length	195

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGGTTGGATTACTAATCCATGTCCTTAGAAAAGTGTCGGCGGGAGACCGGTTCGTAAGTTAAGGACTCTGGCCTACCAAAGGTGATTTTCCTCTCAGATTAAAGGTGCAATTGATTTTTTTTTTTTTTTTTTCCCTTCATCTAATTGCAATTTCCACTTTCTATTCTTCGTCTTCATTTCCAATCACCTCAGTAACAAAAGTGTAGTTGAATTTGGATGTTTTTAGATTGATGAATTTGAAGGTTAAAAGTTTTCACAGTTGAGCGGCAAGCAAATAGGGATTAGTTGGATTGGGAAAAATAGGCTCTGAAGTTGCCAAAAGGCTGGAGGGTTTTGGGTGCAGAATCTCATATAACTCAAGGACCAAGAAGCCAGTAGTTCCATACTCATTAACAGGGAGGTGA

mRNA sequence

ATGGCGGTTGGATTACTAATCCATGTCCTTAGAAAAGTGTCGGCGGGAGACCGATTGATGAATTTGAAGGGATTAGTTGGATTGGGAAAAATAGGCTCTGAAGTTGCCAAAAGGCTGGAGGGTTTTGGGTGCAGAATCTCATATAACTCAAGGACCAAGAAGCCAGTAGTTCCATACTCATTAACAGGGAGGTGA

Coding sequence (CDS)

ATGGCGGTTGGATTACTAATCCATGTCCTTAGAAAAGTGTCGGCGGGAGACCGATTGATGAATTTGAAGGGATTAGTTGGATTGGGAAAAATAGGCTCTGAAGTTGCCAAAAGGCTGGAGGGTTTTGGGTGCAGAATCTCATATAACTCAAGGACCAAGAAGCCAGTAGTTCCATACTCATTAACAGGGAGGTGA

Protein sequence

MAVGLLIHVLRKVSAGDRLMNLKGLVGLGKIGSEVAKRLEGFGCRISYNSRTKKPVVPYSLTGR

Homology

[Close]

BLAST of Carg14818-RA vs. NCBI nr
Match: XP_004134341.2 (PREDICTED: glyoxylate/hydroxypyruvate reductase HPR3-like [Cucumis sativus] >KGN56573.1 hypothetical protein Csa_3G124920 [Cucumis sativus])

HSP 1 Score: 91.7 bits (226), Expect = 1.0e-15
Identity = 53/79 (67.09%), Postives = 56/79 (70.89%), Query Frame = 0


Query: 1   MAVGLLIHVLRKVSAGDRL-------------MNLK------GLVGLGKIGSEVAKRLEG 60
           MAVGLLI VLR VSAGDR              + LK      G+VGLGKIGSEVAKRLEG
Sbjct: 119 MAVGLLIDVLRNVSAGDRFVRQGLWATQMDFSLGLKLTGKRIGIVGLGKIGSEVAKRLEG 178

[Close]

BLAST of Carg14818-RA vs. NCBI nr
Match: XP_022147257.1 (glyoxylate/hydroxypyruvate reductase HPR3-like [Momordica charantia])

HSP 1 Score: 91.7 bits (226), Expect = 1.0e-15
Identity = 53/79 (67.09%), Postives = 56/79 (70.89%), Query Frame = 0


Query: 1   MAVGLLIHVLRKVSAGDRLM-------------NLK------GLVGLGKIGSEVAKRLEG 60
           MAVGLLI VLRKVSAGDR M              LK      G+VGLG+IG EVAKRLEG
Sbjct: 118 MAVGLLIDVLRKVSAGDRFMRQGLWSTKVNFPLGLKLSGKRIGIVGLGRIGCEVAKRLEG 177

[Close]

BLAST of Carg14818-RA vs. NCBI nr
Match: XP_023539897.1 (glyoxylate/hydroxypyruvate reductase HPR3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 91.7 bits (226), Expect = 1.0e-15
Identity = 51/79 (64.56%), Postives = 55/79 (69.62%), Query Frame = 0


Query: 1   MAVGLLIHVLRKVSAGDRLMNLK-------------------GLVGLGKIGSEVAKRLEG 60
           MAVGLLI VLR VSAGDR + L                    G+VGLGKIG+EVAKRLEG
Sbjct: 119 MAVGLLIDVLRNVSAGDRFVRLGLWRTQGDFPLALKLSGKQIGIVGLGKIGTEVAKRLEG 178

[Close]

BLAST of Carg14818-RA vs. NCBI nr
Match: XP_004134340.1 (PREDICTED: glyoxylate/hydroxypyruvate reductase HPR3 [Cucumis sativus] >KGN56571.1 hypothetical protein Csa_3G124900 [Cucumis sativus])

HSP 1 Score: 90.9 bits (224), Expect = 1.7e-15
Identity = 51/79 (64.56%), Postives = 55/79 (69.62%), Query Frame = 0


Query: 1   MAVGLLIHVLRKVSAGDRLMNLK-------------------GLVGLGKIGSEVAKRLEG 60
           MAVGLLI VLRKVSAGDR +  +                   G+VGLGKIGSEVAKRLEG
Sbjct: 117 MAVGLLIDVLRKVSAGDRFVKQRLQPIKPDFPLRSKLSGKQIGIVGLGKIGSEVAKRLEG 176

[Close]

BLAST of Carg14818-RA vs. NCBI nr
Match: XP_008438122.1 (PREDICTED: glyoxylate/hydroxypyruvate reductase HPR3-like [Cucumis melo])

HSP 1 Score: 90.9 bits (224), Expect = 1.7e-15
Identity = 52/79 (65.82%), Postives = 56/79 (70.89%), Query Frame = 0


Query: 1   MAVGLLIHVLRKVSAGDRL-------------MNLK------GLVGLGKIGSEVAKRLEG 60
           MAVGLLI VLR +SAGDR              + LK      G+VGLGKIGSEVAKRLEG
Sbjct: 119 MAVGLLIDVLRNISAGDRFVRQGLWPTQGDFSIGLKLSGKRIGIVGLGKIGSEVAKRLEG 178

[Close]

BLAST of Carg14818-RA vs. TAIR10
Match: AT1G12550.1 (D-isomer specific 2-hydroxyacid dehydrogenase family protein)

HSP 1 Score: 63.2 bits (152), Expect = 6.9e-11
Identity = 38/77 (49.35%), Postives = 44/77 (57.14%), Query Frame = 0


Query: 2   AVGLLIHVLRKVSAGDRLM-------------------NLKGLVGLGKIGSEVAKRLEGF 60
           AVGLLI VLR++ A DR +                      G+VGLG IGS VAKRLE F
Sbjct: 115 AVGLLISVLRRIPAADRYVRSGNWAKFGDFQLGSKVSGKRVGIVGLGSIGSFVAKRLESF 174

[Close]

BLAST of Carg14818-RA vs. TAIR10
Match: AT2G45630.2 (D-isomer specific 2-hydroxyacid dehydrogenase family protein)

HSP 1 Score: 62.4 bits (150), Expect = 1.2e-10
Identity = 36/78 (46.15%), Postives = 47/78 (60.26%), Query Frame = 0


Query: 2   AVGLLIHVLRKVSAGDRLMNLK-------------------GLVGLGKIGSEVAKRLEGF 60
           AVGLLI V R++SA +R +  +                   G+VGLG IGS+VA RL+ F
Sbjct: 131 AVGLLIDVFRRISAANRFVKQRFWPLKGDYPLGSKLGRKRIGIVGLGSIGSKVATRLDAF 190

[Close]

BLAST of Carg14818-RA vs. TAIR10
Match: AT5G28310.1 (NAD(P)-binding Rossmann-fold superfamily protein)

HSP 1 Score: 53.5 bits (127), Expect = 5.5e-08
Identity = 25/37 (67.57%), Postives = 31/37 (83.78%), Query Frame = 0


Query: 24  GLVGLGKIGSEVAKRLEGFGCRISYNSRTKKP-VVPY 60
           G+VGLG IGS+VA RL+ FGC+ISY+SR +KP  VPY
Sbjct: 118 GIVGLGSIGSKVATRLKAFGCQISYSSRNRKPYAVPY 154

[Close]

BLAST of Carg14818-RA vs. TAIR10
Match: AT1G79870.1 (D-isomer specific 2-hydroxyacid dehydrogenase family protein)

HSP 1 Score: 50.1 bits (118), Expect = 6.0e-07
Identity = 28/77 (36.36%), Postives = 42/77 (54.55%), Query Frame = 0


Query: 1   MAVGLLIHVLRKVSAGDRLM------------------NLKGLVGLGKIGSEVAKRLEGF 60
           +A+GL++ +LR++   DR +                     G++GLG+IG+ +AKR E F
Sbjct: 107 LAIGLILALLRRLCECDRYVRSGKWKQGEFQLTTKFSGKSVGIIGLGRIGTAIAKRAEAF 166

[Close]

BLAST of Carg14818-RA vs. Swiss-Prot
Match: sp|Q9LE33|HPR3_ARATH (Glyoxylate/hydroxypyruvate reductase HPR3 OS=Arabidopsis thaliana OX=3702 GN=HPR3 PE=2 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 1.2e-09
Identity = 38/77 (49.35%), Postives = 44/77 (57.14%), Query Frame = 0


Query: 2   AVGLLIHVLRKVSAGDRLM-------------------NLKGLVGLGKIGSEVAKRLEGF 60
           AVGLLI VLR++ A DR +                      G+VGLG IGS VAKRLE F
Sbjct: 115 AVGLLISVLRRIPAADRYVRSGNWAKFGDFQLGSKVSGKRVGIVGLGSIGSFVAKRLESF 174

[Close]

BLAST of Carg14818-RA vs. Swiss-Prot
Match: sp|Q65CJ7|HPPR_PLESU (Hydroxyphenylpyruvate reductase OS=Plectranthus scutellarioides OX=4142 GN=HPPR PE=1 SV=2)

HSP 1 Score: 50.8 bits (120), Expect = 6.4e-06
Identity = 31/81 (38.27%), Postives = 44/81 (54.32%), Query Frame = 0


Query: 1   MAVGLLIHVLRKV------------SAGDRLMNLK------GLVGLGKIGSEVAKRLEGF 60
           +A+GL++ VLR++              GD  +  K      G++GLG+IG  VA+R E F
Sbjct: 107 LAIGLILAVLRRICECDKYVRRGAWKFGDFKLTTKFSGKRVGIIGLGRIGLAVAERAEAF 166

Query: 61  GCRISYNSRTKKPVVPYSLTG 64
            C ISY SR+KKP   Y+  G
Sbjct: 167 DCPISYFSRSKKPNTNYTYYG 187

[Close]

BLAST of Carg14818-RA vs. Swiss-Prot
Match: sp|B6YWH0|GYAR_THEON (Glyoxylate reductase OS=Thermococcus onnurineus (strain NA1) OX=523850 GN=gyaR PE=3 SV=1)

HSP 1 Score: 50.1 bits (118), Expect = 1.1e-05
Identity = 22/38 (57.89%), Postives = 28/38 (73.68%), Query Frame = 0


Query: 24  GLVGLGKIGSEVAKRLEGFGCRISYNSRTKKPVVPYSL 62
           G+VG G+IG  +A+R +GFG RI YNSRT+KP V   L
Sbjct: 154 GIVGFGRIGQAIARRAKGFGMRILYNSRTRKPEVEKEL 191

[Close]

BLAST of Carg14818-RA vs. Swiss-Prot
Match: sp|Q9CA90|HPR2_ARATH (Glyoxylate/hydroxypyruvate reductase A HPR2 OS=Arabidopsis thaliana OX=3702 GN=HPR2 PE=1 SV=1)

HSP 1 Score: 50.1 bits (118), Expect = 1.1e-05
Identity = 28/77 (36.36%), Postives = 42/77 (54.55%), Query Frame = 0


Query: 1   MAVGLLIHVLRKVSAGDRLM------------------NLKGLVGLGKIGSEVAKRLEGF 60
           +A+GL++ +LR++   DR +                     G++GLG+IG+ +AKR E F
Sbjct: 107 LAIGLILALLRRLCECDRYVRSGKWKQGEFQLTTKFSGKSVGIIGLGRIGTAIAKRAEAF 166

[Close]

BLAST of Carg14818-RA vs. Swiss-Prot
Match: sp|C5A1V0|GYAR_THEGJ (Glyoxylate reductase OS=Thermococcus gammatolerans (strain DSM 15229 / JCM 11827 / EJ3) OX=593117 GN=gyaR PE=3 SV=1)

HSP 1 Score: 45.4 bits (106), Expect = 2.7e-04
Identity = 19/32 (59.38%), Postives = 25/32 (78.12%), Query Frame = 0


Query: 24  GLVGLGKIGSEVAKRLEGFGCRISYNSRTKKP 56
           G++G G+IG  VA+R +GFG RI Y SRT+KP
Sbjct: 154 GIIGFGRIGQAVARRAKGFGMRILYYSRTRKP 185

[Close]

BLAST of Carg14818-RA vs. TrEMBL
Match: tr|A0A0A0L4C2|A0A0A0L4C2_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G124920 PE=3 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 6.6e-16
Identity = 53/79 (67.09%), Postives = 56/79 (70.89%), Query Frame = 0


Query: 1   MAVGLLIHVLRKVSAGDRL-------------MNLK------GLVGLGKIGSEVAKRLEG 60
           MAVGLLI VLR VSAGDR              + LK      G+VGLGKIGSEVAKRLEG
Sbjct: 119 MAVGLLIDVLRNVSAGDRFVRQGLWATQMDFSLGLKLTGKRIGIVGLGKIGSEVAKRLEG 178

[Close]

BLAST of Carg14818-RA vs. TrEMBL
Match: tr|A0A1S3AW95|A0A1S3AW95_CUCME (glyoxylate/hydroxypyruvate reductase HPR3-like OS=Cucumis melo OX=3656 GN=LOC103483323 PE=3 SV=1)

HSP 1 Score: 90.9 bits (224), Expect = 1.1e-15
Identity = 52/79 (65.82%), Postives = 56/79 (70.89%), Query Frame = 0


Query: 1   MAVGLLIHVLRKVSAGDRL-------------MNLK------GLVGLGKIGSEVAKRLEG 60
           MAVGLLI VLR +SAGDR              + LK      G+VGLGKIGSEVAKRLEG
Sbjct: 119 MAVGLLIDVLRNISAGDRFVRQGLWPTQGDFSIGLKLSGKRIGIVGLGKIGSEVAKRLEG 178

[Close]

BLAST of Carg14818-RA vs. TrEMBL
Match: tr|A0A0A0L908|A0A0A0L908_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G124900 PE=3 SV=1)

HSP 1 Score: 90.9 bits (224), Expect = 1.1e-15
Identity = 51/79 (64.56%), Postives = 55/79 (69.62%), Query Frame = 0


Query: 1   MAVGLLIHVLRKVSAGDRLMNLK-------------------GLVGLGKIGSEVAKRLEG 60
           MAVGLLI VLRKVSAGDR +  +                   G+VGLGKIGSEVAKRLEG
Sbjct: 117 MAVGLLIDVLRKVSAGDRFVKQRLQPIKPDFPLRSKLSGKQIGIVGLGKIGSEVAKRLEG 176

[Close]

BLAST of Carg14818-RA vs. TrEMBL
Match: tr|A0A1S3AW81|A0A1S3AW81_CUCME (glyoxylate/hydroxypyruvate reductase HPR3-like OS=Cucumis melo OX=3656 GN=LOC103483325 PE=3 SV=1)

HSP 1 Score: 87.0 bits (214), Expect = 1.6e-14
Identity = 50/79 (63.29%), Postives = 55/79 (69.62%), Query Frame = 0


Query: 1   MAVGLLIHVLRKVSAGDRLM-------------NLK------GLVGLGKIGSEVAKRLEG 60
           MAVGLLI VLRKVSAGDR +              LK      G+VGLGKIGSEVAKRLEG
Sbjct: 112 MAVGLLIDVLRKVSAGDRFVRQGLWSKKGDFPPGLKLSGKRIGIVGLGKIGSEVAKRLEG 171

[Close]

BLAST of Carg14818-RA vs. TrEMBL
Match: tr|A0A1S3AYM4|A0A1S3AYM4_CUCME (glyoxylate/hydroxypyruvate reductase HPR3-like OS=Cucumis melo OX=3656 GN=LOC103484170 PE=3 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 2.8e-14
Identity = 48/79 (60.76%), Postives = 54/79 (68.35%), Query Frame = 0


Query: 1   MAVGLLIHVLRKVSAGDRLMNLK-------------------GLVGLGKIGSEVAKRLEG 60
           MAVGLLI VLRKVSAGDR +  +                   G+VGLGKIG+EVA+RLEG
Sbjct: 114 MAVGLLIDVLRKVSAGDRFVKQRLQPTKLHFPLGSKLSGKQIGIVGLGKIGTEVARRLEG 173

The following BLAST results are available for this feature:

BLAST of Carg14818-RA vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004134341.2	1.0e-15	67.09	PREDICTED: glyoxylate/hydroxypyruvate reductase HPR3-like [Cucumis sativus] >KGN...	[more]
XP_022147257.1	1.0e-15	67.09	glyoxylate/hydroxypyruvate reductase HPR3-like [Momordica charantia]	[more]
XP_023539897.1	1.0e-15	64.56	glyoxylate/hydroxypyruvate reductase HPR3-like [Cucurbita pepo subsp. pepo]	[more]
XP_004134340.1	1.7e-15	64.56	PREDICTED: glyoxylate/hydroxypyruvate reductase HPR3 [Cucumis sativus] >KGN56571...	[more]
XP_008438122.1	1.7e-15	65.82	PREDICTED: glyoxylate/hydroxypyruvate reductase HPR3-like [Cucumis melo]	[more]

BLAST of Carg14818-RA vs. TAIR10


Match Name	E-value	Identity	Description
AT1G12550.1	6.9e-11	49.35	D-isomer specific 2-hydroxyacid dehydrogenase family protein	[more]
AT2G45630.2	1.2e-10	46.15	D-isomer specific 2-hydroxyacid dehydrogenase family protein	[more]
AT5G28310.1	5.5e-08	67.57	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT1G79870.1	6.0e-07	36.36	D-isomer specific 2-hydroxyacid dehydrogenase family protein	[more]

BLAST of Carg14818-RA vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q9LE33\|HPR3_ARATH	1.2e-09	49.35	Glyoxylate/hydroxypyruvate reductase HPR3 OS=Arabidopsis thaliana OX=3702 GN=HPR...	[more]
sp\|Q65CJ7\|HPPR_PLESU	6.4e-06	38.27	Hydroxyphenylpyruvate reductase OS=Plectranthus scutellarioides OX=4142 GN=HPPR ...	[more]
sp\|B6YWH0\|GYAR_THEON	1.1e-05	57.89	Glyoxylate reductase OS=Thermococcus onnurineus (strain NA1) OX=523850 GN=gyaR P...	[more]
sp\|Q9CA90\|HPR2_ARATH	1.1e-05	36.36	Glyoxylate/hydroxypyruvate reductase A HPR2 OS=Arabidopsis thaliana OX=3702 GN=H...	[more]
sp\|C5A1V0\|GYAR_THEGJ	2.7e-04	59.38	Glyoxylate reductase OS=Thermococcus gammatolerans (strain DSM 15229 / JCM 11827...	[more]

BLAST of Carg14818-RA vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A0A0L4C2\|A0A0A0L4C2_CUCSA	6.6e-16	67.09	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G124920 PE=3 SV=1	[more]
tr\|A0A1S3AW95\|A0A1S3AW95_CUCME	1.1e-15	65.82	glyoxylate/hydroxypyruvate reductase HPR3-like OS=Cucumis melo OX=3656 GN=LOC103...	[more]
tr\|A0A0A0L908\|A0A0A0L908_CUCSA	1.1e-15	64.56	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G124900 PE=3 SV=1	[more]
tr\|A0A1S3AW81\|A0A1S3AW81_CUCME	1.6e-14	63.29	glyoxylate/hydroxypyruvate reductase HPR3-like OS=Cucumis melo OX=3656 GN=LOC103...	[more]
tr\|A0A1S3AYM4\|A0A1S3AYM4_CUCME	2.8e-14	60.76	glyoxylate/hydroxypyruvate reductase HPR3-like OS=Cucumis melo OX=3656 GN=LOC103...	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **Biological Process**
Term	Definition
GO:0055114	oxidation-reduction process

Vocabulary: **Molecular Function**
Term	Definition
GO:0051287	NAD binding

Vocabulary: **INTERPRO**
Term	Definition
IPR036291	NAD(P)-bd_dom_sf
IPR006140	D-isomer_DH_NAD-bd

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055114	oxidation-reduction process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0051287	NAD binding

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Carg14818	Carg14818	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Carg14818-RA	Carg14818-RA-protein	polypeptide

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg14818-RA.CDS.3	Carg14818-RA.CDS.3	CDS
Carg14818-RA.CDS.2	Carg14818-RA.CDS.2	CDS
Carg14818-RA.CDS.1	Carg14818-RA.CDS.1	CDS

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg14818-RA.exon.3	Carg14818-RA.exon.3	exon
Carg14818-RA.exon.2	Carg14818-RA.exon.2	exon
Carg14818-RA.exon.1	Carg14818-RA.exon.1	exon

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006140	D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding domain	PFAM	PF02826	2-Hacid_dh_C	coord: 24..55 e-value: 9.5E-8 score: 31.5
None	No IPR available	GENE3D	G3DSA:3.40.50.720		coord: 24..61 e-value: 4.0E-11 score: 44.2
None	No IPR available	PANTHER	PTHR10996	2-HYDROXYACID DEHYDROGENASE-RELATED	coord: 24..60
None	No IPR available	PANTHER	PTHR10996:SF204	SUBFAMILY NOT NAMED	coord: 24..60
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	SSF51735	NAD(P)-binding Rossmann-fold domains	coord: 2..57