Carg13586-RA (mRNA) Silver-seed gourd

NameCarg13586-RA
TypemRNA
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionRiboflavin synthase
LocationCucurbita_argyrosperma_scaffold_053 : 364060 .. 364908 (-)
Sequence length849
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAACTTTTTCTTCGCTCTCGGCGGCGACCGCACTGTCCAGCATCCCGAAAATGGCTTCTCCGGCGAGGAACCCAATTCCCAATGTTCATCCCGCATCGTCTCAATTCTGCCCTAATTTGAAATTCAATCCCAAAAGATCTCTGTTCGTTGTATTCACCTCTAGTCAGAAGCCCCAATTTCCGTGCAAATCTCGGCGGAGGAGCTCCGTCCAATGCCTTTTCACCGGCATAGTTGAAGAAATGGGGCGTGTAAAAAACTTGGGTATTGCTGAAAATGGGGGTTTTGATTTGGAAATCACAGCGAAAACTGTTCTTCAAGGCGTTAACCTTGGAGACAGTATTGCTGTGAATGGAACTTGCCTTACGGTGACGGCTTTTGACCACAAACTATCTGAATTCAAGGTGGGGTTAGCGCCGGAGACGCTCAGGAAGACGTCGTTGGTGGAGCTGGTGCCGGGTTCTCCGGTGAATTTGGAGCGGGCGGTTCAGCCAATCAGCCGAATGGGTGGGCATTTTGTGCAGGGGCATGTGGATGGGACAGGGGAGATAGTGTCGATGGATGCTGAGGGGGACTCGTTGTGGGTAAAGGTGAGGACGTCGGAGGCTGTGCTGAACTATATTGTAGCAAAGGGATTCATTGCGGTGGATGGAACTAGCTTGACTGTGGTGGATGTTTTTGATGAGGAAAAGGCTTTTAACTTCATGTTGGTTGCTTATACTCAGAATGCTGTGGTGGTTCCATTGAAGAAAGTGGGGGATTTGGTGAATTTGGAGGTTGATGTTCTTGGCAAGTATGTGCAGCGGCTCCTTAGCAATGGTGCCATCGGTTCCATCGAGTCTCAGTGA

mRNA sequence

ATGGCAACTTTTTCTTCGCTCTCGGCGGCGACCGCACTGTCCAGCATCCCGAAAATGGCTTCTCCGGCGAGGAACCCAATTCCCAATGTTCATCCCGCATCGTCTCAATTCTGCCCTAATTTGAAATTCAATCCCAAAAGATCTCTGTTCGTTGTATTCACCTCTAGTCAGAAGCCCCAATTTCCGTGCAAATCTCGGCGGAGGAGCTCCGTCCAATGCCTTTTCACCGGCATAGTTGAAGAAATGGGGCGTGTAAAAAACTTGGGTATTGCTGAAAATGGGGGTTTTGATTTGGAAATCACAGCGAAAACTGTTCTTCAAGGCGTTAACCTTGGAGACAGTATTGCTGTGAATGGAACTTGCCTTACGGTGACGGCTTTTGACCACAAACTATCTGAATTCAAGGTGGGGTTAGCGCCGGAGACGCTCAGGAAGACGTCGTTGGTGGAGCTGGTGCCGGGTTCTCCGGTGAATTTGGAGCGGGCGGTTCAGCCAATCAGCCGAATGGGTGGGCATTTTGTGCAGGGGCATGTGGATGGGACAGGGGAGATAGTGTCGATGGATGCTGAGGGGGACTCGTTGTGGGTAAAGGTGAGGACGTCGGAGGCTGTGCTGAACTATATTGTAGCAAAGGGATTCATTGCGGTGGATGGAACTAGCTTGACTGTGGTGGATGTTTTTGATGAGGAAAAGGCTTTTAACTTCATGTTGGTTGCTTATACTCAGAATGCTGTGGTGGTTCCATTGAAGAAAGTGGGGGATTTGGTGAATTTGGAGGTTGATGTTCTTGGCAAGTATGTGCAGCGGCTCCTTAGCAATGGTGCCATCGGTTCCATCGAGTCTCAGTGA

Coding sequence (CDS)

ATGGCAACTTTTTCTTCGCTCTCGGCGGCGACCGCACTGTCCAGCATCCCGAAAATGGCTTCTCCGGCGAGGAACCCAATTCCCAATGTTCATCCCGCATCGTCTCAATTCTGCCCTAATTTGAAATTCAATCCCAAAAGATCTCTGTTCGTTGTATTCACCTCTAGTCAGAAGCCCCAATTTCCGTGCAAATCTCGGCGGAGGAGCTCCGTCCAATGCCTTTTCACCGGCATAGTTGAAGAAATGGGGCGTGTAAAAAACTTGGGTATTGCTGAAAATGGGGGTTTTGATTTGGAAATCACAGCGAAAACTGTTCTTCAAGGCGTTAACCTTGGAGACAGTATTGCTGTGAATGGAACTTGCCTTACGGTGACGGCTTTTGACCACAAACTATCTGAATTCAAGGTGGGGTTAGCGCCGGAGACGCTCAGGAAGACGTCGTTGGTGGAGCTGGTGCCGGGTTCTCCGGTGAATTTGGAGCGGGCGGTTCAGCCAATCAGCCGAATGGGTGGGCATTTTGTGCAGGGGCATGTGGATGGGACAGGGGAGATAGTGTCGATGGATGCTGAGGGGGACTCGTTGTGGGTAAAGGTGAGGACGTCGGAGGCTGTGCTGAACTATATTGTAGCAAAGGGATTCATTGCGGTGGATGGAACTAGCTTGACTGTGGTGGATGTTTTTGATGAGGAAAAGGCTTTTAACTTCATGTTGGTTGCTTATACTCAGAATGCTGTGGTGGTTCCATTGAAGAAAGTGGGGGATTTGGTGAATTTGGAGGTTGATGTTCTTGGCAAGTATGTGCAGCGGCTCCTTAGCAATGGTGCCATCGGTTCCATCGAGTCTCAGTGA

Protein sequence

MATFSSLSAATALSSIPKMASPARNPIPNVHPASSQFCPNLKFNPKRSLFVVFTSSQKPQFPCKSRRRSSVQCLFTGIVEEMGRVKNLGIAENGGFDLEITAKTVLQGVNLGDSIAVNGTCLTVTAFDHKLSEFKVGLAPETLRKTSLVELVPGSPVNLERAVQPISRMGGHFVQGHVDGTGEIVSMDAEGDSLWVKVRTSEAVLNYIVAKGFIAVDGTSLTVVDVFDEEKAFNFMLVAYTQNAVVVPLKKVGDLVNLEVDVLGKYVQRLLSNGAIGSIESQ
BLAST of Carg13586-RA vs. NCBI nr
Match: XP_022947746.1 (riboflavin synthase-like [Cucurbita moschata])

HSP 1 Score: 544.3 bits (1401), Expect = 2.5e-151
Identity = 281/282 (99.65%), Postives = 281/282 (99.65%), Query Frame = 0

Query: 1   MATFSSLSAATALSSIPKMASPARNPIPNVHPASSQFCPNLKFNPKRSLFVVFTSSQKPQ 60
           MATFSSLSAATALSSIPKMASPARNPIPNVHPASSQFCP LKFNPKRSLFVVFTSSQKPQ
Sbjct: 1   MATFSSLSAATALSSIPKMASPARNPIPNVHPASSQFCPFLKFNPKRSLFVVFTSSQKPQ 60

Query: 61  FPCKSRRRSSVQCLFTGIVEEMGRVKNLGIAENGGFDLEITAKTVLQGVNLGDSIAVNGT 120
           FPCKSRRRSSVQCLFTGIVEEMGRVKNLGIAENGGFDLEITAKTVLQGVNLGDSIAVNGT
Sbjct: 61  FPCKSRRRSSVQCLFTGIVEEMGRVKNLGIAENGGFDLEITAKTVLQGVNLGDSIAVNGT 120

Query: 121 CLTVTAFDHKLSEFKVGLAPETLRKTSLVELVPGSPVNLERAVQPISRMGGHFVQGHVDG 180
           CLTVTAFDHKLSEFKVGLAPETLRKTSLVELVPGSPVNLERAVQPISRMGGHFVQGHVDG
Sbjct: 121 CLTVTAFDHKLSEFKVGLAPETLRKTSLVELVPGSPVNLERAVQPISRMGGHFVQGHVDG 180

Query: 181 TGEIVSMDAEGDSLWVKVRTSEAVLNYIVAKGFIAVDGTSLTVVDVFDEEKAFNFMLVAY 240
           TGEIVSMDAEGDSLWVKVRTSEAVLNYIVAKGFIAVDGTSLTVVDVFDEEKAFNFMLVAY
Sbjct: 181 TGEIVSMDAEGDSLWVKVRTSEAVLNYIVAKGFIAVDGTSLTVVDVFDEEKAFNFMLVAY 240

Query: 241 TQNAVVVPLKKVGDLVNLEVDVLGKYVQRLLSNGAIGSIESQ 283
           TQNAVVVPLKKVGDLVNLEVDVLGKYVQRLLSNGAIGSIESQ
Sbjct: 241 TQNAVVVPLKKVGDLVNLEVDVLGKYVQRLLSNGAIGSIESQ 282

BLAST of Carg13586-RA vs. NCBI nr
Match: XP_023532107.1 (riboflavin synthase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 541.6 bits (1394), Expect = 1.6e-150
Identity = 277/282 (98.23%), Postives = 280/282 (99.29%), Query Frame = 0

Query: 1   MATFSSLSAATALSSIPKMASPARNPIPNVHPASSQFCPNLKFNPKRSLFVVFTSSQKPQ 60
           M TFSSLSAATALS IPKMASPARNPIPNVHPASSQFCPNLKFNPKRSLFVVFTSSQKPQ
Sbjct: 1   MPTFSSLSAATALSGIPKMASPARNPIPNVHPASSQFCPNLKFNPKRSLFVVFTSSQKPQ 60

Query: 61  FPCKSRRRSSVQCLFTGIVEEMGRVKNLGIAENGGFDLEITAKTVLQGVNLGDSIAVNGT 120
           FPCKSRRRSSVQCLFTGIVEEMGRVKNLGIAENGGFDLEITAKTVL+GVNLGDSIAVNGT
Sbjct: 61  FPCKSRRRSSVQCLFTGIVEEMGRVKNLGIAENGGFDLEITAKTVLEGVNLGDSIAVNGT 120

Query: 121 CLTVTAFDHKLSEFKVGLAPETLRKTSLVELVPGSPVNLERAVQPISRMGGHFVQGHVDG 180
           CLTVTAFDHKLSEFKVGLAPETLRKTSLVELVPGSPVNLERAVQPISRMGGHFVQGHVDG
Sbjct: 121 CLTVTAFDHKLSEFKVGLAPETLRKTSLVELVPGSPVNLERAVQPISRMGGHFVQGHVDG 180

Query: 181 TGEIVSMDAEGDSLWVKVRTSEAVLNYIVAKGFIAVDGTSLTVVDVFDEEKAFNFMLVAY 240
           TGEIVSMDAEGDSLWVKVRTSEAVLNYIVAKGFIAVDGTSLTVVDVFDEEKAFNFMLVAY
Sbjct: 181 TGEIVSMDAEGDSLWVKVRTSEAVLNYIVAKGFIAVDGTSLTVVDVFDEEKAFNFMLVAY 240

Query: 241 TQNAVVVPLKKVGDLVNLEVDVLGKYVQRLLSNGAIGSIESQ 283
           TQNAVVVPLKKVGDLVNLEVD+LGKYVQRLL+NGAIGSIESQ
Sbjct: 241 TQNAVVVPLKKVGDLVNLEVDILGKYVQRLLTNGAIGSIESQ 282

BLAST of Carg13586-RA vs. NCBI nr
Match: XP_022970720.1 (riboflavin synthase-like [Cucurbita maxima])

HSP 1 Score: 527.3 bits (1357), Expect = 3.1e-146
Identity = 273/281 (97.15%), Postives = 274/281 (97.51%), Query Frame = 0

Query: 1   MATFSSLSAATALSSIPKMASPARNPIPNVHPASSQFCPNLKFNPKRSLFVVFTSSQKPQ 60
           MATF SLSAATALSSIPKMASPARNP PNVHPASSQFCPN KFNPKRSLFVVFTSSQKPQ
Sbjct: 1   MATFPSLSAATALSSIPKMASPARNPTPNVHPASSQFCPNFKFNPKRSLFVVFTSSQKPQ 60

Query: 61  FPCKSRRRSSVQCLFTGIVEEMGRVKNLGIAENGGFDLEITAKTVLQGVNLGDSIAVNGT 120
           FPCKSR R SVQCLFTGIVEEMGRVKNLGIAENGGFDLEITAKTVLQGVNLGDSIAVNGT
Sbjct: 61  FPCKSRPRISVQCLFTGIVEEMGRVKNLGIAENGGFDLEITAKTVLQGVNLGDSIAVNGT 120

Query: 121 CLTVTAFDHKLSEFKVGLAPETLRKTSLVELVPGSPVNLERAVQPISRMGGHFVQGHVDG 180
           CLTVTAFDH+LSEFKVGLAPETLRKTSLVELVPGSPVNLERAVQPISRMGGHFVQGHVDG
Sbjct: 121 CLTVTAFDHQLSEFKVGLAPETLRKTSLVELVPGSPVNLERAVQPISRMGGHFVQGHVDG 180

Query: 181 TGEIVSMDAEGDSLWVKVRTSEAVLNYIVAKGFIAVDGTSLTVVDVFDEEKAFNFMLVAY 240
           TGEIVSMDAE DSLWVKVRTSEAVLNYIVAKGFIAVDGTSLTVVDVFDEEKAFNFMLVAY
Sbjct: 181 TGEIVSMDAEEDSLWVKVRTSEAVLNYIVAKGFIAVDGTSLTVVDVFDEEKAFNFMLVAY 240

Query: 241 TQNAVVVPLKKVGDLVNLEVDVLGKYVQRLLSNGAIGSIES 282
           TQNAVVVPLKKVGDLVNLEVDVLGKYVQRLLS GAIGSIES
Sbjct: 241 TQNAVVVPLKKVGDLVNLEVDVLGKYVQRLLSIGAIGSIES 281

BLAST of Carg13586-RA vs. NCBI nr
Match: XP_022140671.1 (LOW QUALITY PROTEIN: riboflavin synthase [Momordica charantia] >AFZ99647.1 riboflavin synthase [Momordica charantia])

HSP 1 Score: 455.7 bits (1171), Expect = 1.2e-124
Identity = 233/281 (82.92%), Postives = 249/281 (88.61%), Query Frame = 0

Query: 1   MATFSSLSAATALSSIPKMASPARNPIPNVHPASSQFCPNLKFNPKRSLFVVFTSSQKPQ 60
           M+TFSSLS ATALS IPKM  P  N I NV P S  FC   KFN  RSLF +FTSS KPQ
Sbjct: 1   MSTFSSLSTATALSQIPKMVPPGGNTILNVRPGSPHFCLKFKFNTNRSLFTLFTSSCKPQ 60

Query: 61  FPCKSRRRSSVQCLFTGIVEEMGRVKNLGIAENGGFDLEITAKTVLQGVNLGDSIAVNGT 120
             CKSR R+S+QCLFTGIVEEMGRVKNLG+ ENGGFDLEI AKTVL+GV+LGDSIAVNGT
Sbjct: 61  LRCKSRPRTSIQCLFTGIVEEMGRVKNLGVTENGGFDLEIVAKTVLEGVHLGDSIAVNGT 120

Query: 121 CLTVTAFDHKLSEFKVGLAPETLRKTSLVELVPGSPVNLERAVQPISRMGGHFVQGHVDG 180
           CLTVTAFDH+LSEF VGLAPETLRKTSLVELVPGSPVNLERAVQPISRMGGHFVQGHVDG
Sbjct: 121 CLTVTAFDHELSEFTVGLAPETLRKTSLVELVPGSPVNLERAVQPISRMGGHFVQGHVDG 180

Query: 181 TGEIVSMDAEGDSLWVKVRTSEAVLNYIVAKGFIAVDGTSLTVVDVFDEEKAFNFMLVAY 240
           TGEI+SM+ E DSLW+KVRTSE +L YIV KGFIAVDGTSLTVVDVFDEEKAFNFMLVAY
Sbjct: 181 TGEIISMEPEEDSLWIKVRTSEDLLKYIVPKGFIAVDGTSLTVVDVFDEEKAFNFMLVAY 240

Query: 241 TQNAVVVPLKKVGDLVNLEVDVLGKYVQRLLSNGAIGSIES 282
           TQN+VV+PLKKVGDLVNLEVD+LGKYVQRLLSNGAI  I+S
Sbjct: 241 TQNSVVIPLKKVGDLVNLEVDILGKYVQRLLSNGAITPIKS 281

BLAST of Carg13586-RA vs. NCBI nr
Match: XP_022986014.1 (riboflavin synthase-like [Cucurbita maxima])

HSP 1 Score: 453.4 bits (1165), Expect = 5.8e-124
Identity = 231/281 (82.21%), Postives = 250/281 (88.97%), Query Frame = 0

Query: 1   MATFSSLSAATALSSIPKMASPARNPIPNVHPASSQFCPNLKFNPKRSLFVVFTSSQKPQ 60
           MATFSSLS A +LS+IPKM  P R+ I NVHP+SSQFCP  + N  RSLF +FTS +KPQ
Sbjct: 1   MATFSSLSTAISLSNIPKMVPPRRDAILNVHPSSSQFCPRFELNTNRSLFTLFTSCRKPQ 60

Query: 61  FPCKSRRRSSVQCLFTGIVEEMGRVKNLGIAENGGFDLEITAKTVLQGVNLGDSIAVNGT 120
             CK R RSSVQCLFTGIVEE+G VK +GIAE+GGFDL+ITAKTVL+GVNLGDSI+VNGT
Sbjct: 61  LRCKPRPRSSVQCLFTGIVEEIGHVKQVGIAEHGGFDLKITAKTVLEGVNLGDSISVNGT 120

Query: 121 CLTVTAFDHKLSEFKVGLAPETLRKTSLVELVPGSPVNLERAVQPISRMGGHFVQGHVDG 180
           CLTVTAFDH+LSEFKVGLAPETLRKTSLVEL  GSPVNLERAVQPISRMGGHFVQGHVDG
Sbjct: 121 CLTVTAFDHQLSEFKVGLAPETLRKTSLVELTAGSPVNLERAVQPISRMGGHFVQGHVDG 180

Query: 181 TGEIVSMDAEGDSLWVKVRTSEAVLNYIVAKGFIAVDGTSLTVVDVFDEEKAFNFMLVAY 240
           TGEI+SMD EGDSLW+KVRTSEA+L YIV KGFIAVDGTSLTVVDVFDEEKAFNFMLVAY
Sbjct: 181 TGEIISMDPEGDSLWIKVRTSEALLKYIVPKGFIAVDGTSLTVVDVFDEEKAFNFMLVAY 240

Query: 241 TQNAVVVPLKKVGDLVNLEVDVLGKYVQRLLSNGAIGSIES 282
           TQ  VV+PLK VG LVNLEVD+LGKYVQRLLSNGAI  IES
Sbjct: 241 TQKNVVIPLKNVGQLVNLEVDILGKYVQRLLSNGAIKPIES 281

BLAST of Carg13586-RA vs. TAIR10
Match: AT2G20690.1 (lumazine-binding family protein)

HSP 1 Score: 298.9 bits (764), Expect = 3.3e-81
Identity = 148/209 (70.81%), Postives = 176/209 (84.21%), Query Frame = 0

Query: 66  RRRSSVQCLFTGIVEEMGRVKNLGIAENGGFDLEITAKTVLQGVNLGDSIAVNGTCLTVT 125
           R+  S++ +FTGIVEEMG VK+LG+A++GGFDL+I A+ VL+ V LGDSIAVNGTCLTVT
Sbjct: 56  RQNLSIRSVFTGIVEEMGEVKDLGMADHGGFDLKIGARVVLEDVKLGDSIAVNGTCLTVT 115

Query: 126 AFDHKLSEFKVGLAPETLRKTSLVELVPGSPVNLERAVQPISRMGGHFVQGHVDGTGEIV 185
            F+    EF VGLAPETLRKTSL EL  GSPVNLERA+QP+SRMGGH VQGHVDGTG I 
Sbjct: 116 EFN--AEEFTVGLAPETLRKTSLEELKKGSPVNLERALQPVSRMGGHVVQGHVDGTGVIE 175

Query: 186 SMDAEGDSLWVKVRTSEAVLNYIVAKGFIAVDGTSLTVVDVFDEEKAFNFMLVAYTQNAV 245
           SM+ EGDSLWVKV+  + +L YIV KGF+AVDGTSLTVVDVFDEE  FNFM++AYTQ  V
Sbjct: 176 SMEVEGDSLWVKVKADKGLLKYIVPKGFVAVDGTSLTVVDVFDEESCFNFMMIAYTQQNV 235

Query: 246 VVPLKKVGDLVNLEVDVLGKYVQRLLSNG 275
           V+P KK+G  VNLEVD++GKYV+RLL++G
Sbjct: 236 VIPTKKIGQKVNLEVDIMGKYVERLLTSG 262

BLAST of Carg13586-RA vs. Swiss-Prot
Match: sp|Q9Y7P0|RISA_SCHPO (Riboflavin synthase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=rib5 PE=1 SV=1)

HSP 1 Score: 188.7 bits (478), Expect = 8.7e-47
Identity = 101/199 (50.75%), Postives = 138/199 (69.35%), Query Frame = 0

Query: 74  LFTGIVEEMGRVKNLGIAENGGFDLEITAKTVLQGVNLGDSIAVNGTCLTVTAFDHKLSE 133
           +FTG+VE +G VK++    + GF ++I A  +L   + GDSIAVNGTCLTVT FD     
Sbjct: 1   MFTGLVEAIGVVKDVQGTIDNGFAMKIEAPQILDDCHTGDSIAVNGTCLTVTDFDR--YH 60

Query: 134 FKVGLAPETLRKTSLVELVPGSPVNLERAVQPISRMGGHFVQGHVDGTGEIVSMDAEGDS 193
           F VG+APE+LR T+L +   G PVNLERAV   +RMGGHFVQGHVD   EIV    +G++
Sbjct: 61  FTVGIAPESLRLTNLGQCKAGDPVNLERAVLSSTRMGGHFVQGHVDTVAEIVEKKQDGEA 120

Query: 194 LWVKVRTSEA-VLNYIVAKGFIAVDGTSLTVVDVFDEEKAFNFMLVAYTQNAVVVPLKKV 253
           +    R  +  VL YIV KG+IA+DGTSLT+  V  ++  F+ M+++YTQ+ V++  K V
Sbjct: 121 IDFTFRPRDPFVLKYIVYKGYIALDGTSLTITHV--DDSTFSIMMISYTQSKVIMAKKNV 180

Query: 254 GDLVNLEVDVLGKYVQRLL 272
           GDLVN+EVD +GKY ++L+
Sbjct: 181 GDLVNVEVDQIGKYTEKLV 195

BLAST of Carg13586-RA vs. Swiss-Prot
Match: sp|A5DB51|RISA_PICGU (Riboflavin synthase OS=Meyerozyma guilliermondii (strain ATCC 6260 / CBS 566 / DSM 6381 / JCM 1539 / NBRC 10279 / NRRL Y-324) OX=294746 GN=RIB5 PE=2 SV=3)

HSP 1 Score: 184.5 bits (467), Expect = 1.6e-45
Identity = 103/217 (47.47%), Postives = 140/217 (64.52%), Query Frame = 0

Query: 74  LFTGIVEEMGRV-----KNLGIAENGGFDLEI-TAKTVLQGVNLGDSIAVNGTCLTVTAF 133
           +FTG+VE +G V     K+   +   G  + I     +L+ V LGDSI  NG CLTVT F
Sbjct: 1   MFTGLVEHIGTVLQVTEKDTTASGGDGVSMVIGDCSKILEDVQLGDSICTNGVCLTVTEF 60

Query: 134 DHKLSEFKVGLAPETLRKTSLVELVPGSPVNLERAVQPISRMGGHFVQGHVDGTGEIVSM 193
           D   S+FKVG++PETLR++ L EL PGS VNLERAV+   RMGGH VQGHVD    IV+ 
Sbjct: 61  DMARSQFKVGISPETLRRSDLGELKPGSKVNLERAVKADVRMGGHVVQGHVDTIATIVNR 120

Query: 194 DAEGDSL--WVKVRTSEAVLNYIVAKGFIAVDGTSLTVVDVFDEEKAFNFMLVAYTQNAV 253
             +G+++    K+R S+    YIV KGFIA+DGTSLTV DV  E+  F+  +V+YTQ  V
Sbjct: 121 RGDGNAINFTFKLRDSQ-YGKYIVEKGFIAIDGTSLTVTDVDHEQSEFSISMVSYTQEKV 180

Query: 254 VVPLKKVGDLVNLEVDVLGKYVQRLLSNGAIGSIESQ 283
           ++PLK  GD VN+EVD+ GK +++ +    +  I+ +
Sbjct: 181 IMPLKNSGDSVNIEVDLTGKLIEKQIELSLLSYIKDE 216

BLAST of Carg13586-RA vs. Swiss-Prot
Match: sp|P50854|RISA_ACTPL (Riboflavin synthase OS=Actinobacillus pleuropneumoniae OX=715 GN=ribE PE=3 SV=1)

HSP 1 Score: 174.5 bits (441), Expect = 1.7e-42
Identity = 93/199 (46.73%), Postives = 131/199 (65.83%), Query Frame = 0

Query: 74  LFTGIVEEMGRVKNLGIAENGGFD-LEITAKTVLQGVNLGDSIAVNGTCLTVTAFDHKLS 133
           +FTGI+EE+G++    I + G F  + I A  VLQ V+LGD+IAVNG CLTVT+F    +
Sbjct: 1   MFTGIIEEVGKIAQ--IHKQGEFAVVTINATKVLQDVHLGDTIAVNGVCLTVTSFSS--N 60

Query: 134 EFKVGLAPETLRKTSLVELVPGSPVNLERAVQPISRMGGHFVQGHVDGTGEIVSMDAEGD 193
           +F   +  ETL++TSL EL   SPVNLERA+    R GGH V GH+DGTGEI  +    +
Sbjct: 61  QFTADVMSETLKRTSLGELKSNSPVNLERAMAANGRFGGHIVSGHIDGTGEIAEITPAHN 120

Query: 194 SLWVKVRTSEAVLNYIVAKGFIAVDGTSLTVVDVFDEEKAFNFMLVAYTQNAVVVPLKKV 253
           S W +++TS  ++ YI+ KG I +DG SLTVVD  DE  +F   ++ +T     +  KK+
Sbjct: 121 STWYRIKTSPKLMRYIIEKGSITIDGISLTVVDTDDE--SFRVSIIPHTIKETNLGSKKI 180

Query: 254 GDLVNLEVDVLGKYVQRLL 272
           G +VNLE D++GKY+++ L
Sbjct: 181 GSIVNLENDIVGKYIEQFL 193

BLAST of Carg13586-RA vs. Swiss-Prot
Match: sp|P38145|RISA_YEAST (Riboflavin synthase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=RIB5 PE=1 SV=1)

HSP 1 Score: 167.9 bits (424), Expect = 1.6e-40
Identity = 96/206 (46.60%), Postives = 136/206 (66.02%), Query Frame = 0

Query: 74  LFTGIVEEMGRV---KNLGIAENGGFDLEIT---AKTVLQGVNLGDSIAVNGTCLTVTAF 133
           +FTGIVE MG V        +E+GG  + IT   A ++L   ++GDSIAVNG CLTVT F
Sbjct: 1   MFTGIVECMGTVLENNPYDDSESGGQGVSITIGNAGSILTDCHVGDSIAVNGVCLTVTEF 60

Query: 134 DHKLSEFKVGLAPETLRKTSLVELVPGSPVNLERAVQPISRMGGHFVQGHVDGTGEIVSM 193
           ++    FKVG++PET++++++   + G+ VNLERAV    R GGH+VQGHVD    IVS 
Sbjct: 61  NN--DSFKVGISPETIKRSNVASWIQGTQVNLERAVSQDVRFGGHYVQGHVDTVANIVSR 120

Query: 194 DAEGDSL--WVKVRTSEAVLNYIVAKGFIAVDGTSLTV--VDVFDEEKAFNFMLVAYTQN 253
             EG+S+    ++R  E    YIV KGFI +DGTSLT+  VD   +  AF   ++ +TQ+
Sbjct: 121 RPEGNSIIFGFQLRDQE-YFKYIVEKGFICIDGTSLTIIKVDPLSQGGAFYISMIKHTQD 180

Query: 254 AVVVPLKKVGDLVNLEVDVLGKYVQR 270
            V++PLKK+GD VN+EVD+ GK +++
Sbjct: 181 NVIMPLKKIGDEVNIEVDLTGKIIEK 203

BLAST of Carg13586-RA vs. Swiss-Prot
Match: sp|O67604|RISA_AQUAE (Riboflavin synthase OS=Aquifex aeolicus (strain VF5) OX=224324 GN=ribE PE=3 SV=1)

HSP 1 Score: 152.9 bits (385), Expect = 5.3e-36
Identity = 87/199 (43.72%), Postives = 127/199 (63.82%), Query Frame = 0

Query: 74  LFTGIVEEMGRVKNLGIAENGGFDLEITAKTVLQGVNLGDSIAVNGTCLTVTAFDHKLSE 133
           +FTG+VE++G+VKNL ++  G    +++ +T L+ V LGDS++VNG CLTV   D K S 
Sbjct: 1   MFTGLVEDLGKVKNLTLSSKGA---KLSVETKLEDVKLGDSVSVNGACLTVV--DIKSST 60

Query: 134 FKVGLAPETLRKTSLVELVPGSPVNLERAVQPISRMGGHFVQGHVDGTGEIVSMDAEGDS 193
               ++PETL++T+L +L  G  VNLERA++   R+GGH VQGHVD T  + S +  G+ 
Sbjct: 61  LTFDVSPETLKRTNLGKLKTGDYVNLERALRVGERLGGHIVQGHVDFTAPVKSFNFLGEH 120

Query: 194 LWVKVRTSEAVLNYIVAKGFIAVDGTSLTVVDVFDEEKAFNFMLVAYTQNAVVVPLKKVG 253
             + +   E    Y+V KG IA+DG SLT V+   E K F   ++ +T  +  +  KKVG
Sbjct: 121 YELVIEIPEEWSIYVVEKGSIALDGISLT-VNYVKENKVF-INIIPHTYKSTNLQFKKVG 180

Query: 254 DLVNLEVDVLGKYVQRLLS 273
           DL+N+E D+LGKYV   L+
Sbjct: 181 DLLNVETDILGKYVINYLN 192

BLAST of Carg13586-RA vs. TrEMBL
Match: tr|L0C7R1|L0C7R1_MOMCH (Riboflavin synthase OS=Momordica charantia OX=3673 PE=2 SV=1)

HSP 1 Score: 455.7 bits (1171), Expect = 7.7e-125
Identity = 233/281 (82.92%), Postives = 249/281 (88.61%), Query Frame = 0

Query: 1   MATFSSLSAATALSSIPKMASPARNPIPNVHPASSQFCPNLKFNPKRSLFVVFTSSQKPQ 60
           M+TFSSLS ATALS IPKM  P  N I NV P S  FC   KFN  RSLF +FTSS KPQ
Sbjct: 1   MSTFSSLSTATALSQIPKMVPPGGNTILNVRPGSPHFCLKFKFNTNRSLFTLFTSSCKPQ 60

Query: 61  FPCKSRRRSSVQCLFTGIVEEMGRVKNLGIAENGGFDLEITAKTVLQGVNLGDSIAVNGT 120
             CKSR R+S+QCLFTGIVEEMGRVKNLG+ ENGGFDLEI AKTVL+GV+LGDSIAVNGT
Sbjct: 61  LRCKSRPRTSIQCLFTGIVEEMGRVKNLGVTENGGFDLEIVAKTVLEGVHLGDSIAVNGT 120

Query: 121 CLTVTAFDHKLSEFKVGLAPETLRKTSLVELVPGSPVNLERAVQPISRMGGHFVQGHVDG 180
           CLTVTAFDH+LSEF VGLAPETLRKTSLVELVPGSPVNLERAVQPISRMGGHFVQGHVDG
Sbjct: 121 CLTVTAFDHELSEFTVGLAPETLRKTSLVELVPGSPVNLERAVQPISRMGGHFVQGHVDG 180

Query: 181 TGEIVSMDAEGDSLWVKVRTSEAVLNYIVAKGFIAVDGTSLTVVDVFDEEKAFNFMLVAY 240
           TGEI+SM+ E DSLW+KVRTSE +L YIV KGFIAVDGTSLTVVDVFDEEKAFNFMLVAY
Sbjct: 181 TGEIISMEPEEDSLWIKVRTSEDLLKYIVPKGFIAVDGTSLTVVDVFDEEKAFNFMLVAY 240

Query: 241 TQNAVVVPLKKVGDLVNLEVDVLGKYVQRLLSNGAIGSIES 282
           TQN+VV+PLKKVGDLVNLEVD+LGKYVQRLLSNGAI  I+S
Sbjct: 241 TQNSVVIPLKKVGDLVNLEVDILGKYVQRLLSNGAITPIKS 281

BLAST of Carg13586-RA vs. TrEMBL
Match: tr|A0A0A0KAI5|A0A0A0KAI5_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G128550 PE=4 SV=1)

HSP 1 Score: 433.7 bits (1114), Expect = 3.1e-118
Identity = 224/281 (79.72%), Postives = 246/281 (87.54%), Query Frame = 0

Query: 1   MATFSSLSAATALSSIPKMASPARNPIPNVHPASSQFCPNLKFNPKRSLFVVFTSSQKPQ 60
           M+TFSSLS AT+L +IP+M      PI ++H  SS F P   FN K SLF +FTS+ KPQ
Sbjct: 1   MSTFSSLSTATSLPNIPRMLP----PIGSLHLPSSHFFPKFNFNTKTSLFTLFTSTHKPQ 60

Query: 61  FPCKSRRRSSVQCLFTGIVEEMGRVKNLGIAENGGFDLEITAKTVLQGVNLGDSIAVNGT 120
              KSR R+SVQCLFTGIVEE+G VK+LGI+ENGGFDLEI AKTVL+GVNLGDSIAVNGT
Sbjct: 61  LRYKSRPRASVQCLFTGIVEEIGHVKSLGISENGGFDLEINAKTVLEGVNLGDSIAVNGT 120

Query: 121 CLTVTAFDHKLSEFKVGLAPETLRKTSLVELVPGSPVNLERAVQPISRMGGHFVQGHVDG 180
           CLTVTAFDH+LSEFKVGLAPETLRKTSLVEL PGSPVNLERAVQPISRMGGHFVQGHVDG
Sbjct: 121 CLTVTAFDHQLSEFKVGLAPETLRKTSLVELSPGSPVNLERAVQPISRMGGHFVQGHVDG 180

Query: 181 TGEIVSMDAEGDSLWVKVRTSEAVLNYIVAKGFIAVDGTSLTVVDVFDEEKAFNFMLVAY 240
           TGEI+SMD EGDSLW+KV+TSEA+LNYIV KGFIAVDG SLTVVDVFD+EKAFNFMLVAY
Sbjct: 181 TGEIISMDPEGDSLWIKVKTSEALLNYIVPKGFIAVDGASLTVVDVFDDEKAFNFMLVAY 240

Query: 241 TQNAVVVPLKKVGDLVNLEVDVLGKYVQRLLSNGAIGSIES 282
           TQ  VV+PLKKVG LVNLEVD+LGKYVQRLLSNGA+  IES
Sbjct: 241 TQTNVVIPLKKVGHLVNLEVDILGKYVQRLLSNGAVKPIES 277

BLAST of Carg13586-RA vs. TrEMBL
Match: tr|A0A1S3CSC8|A0A1S3CSC8_CUCME (riboflavin synthase OS=Cucumis melo OX=3656 GN=LOC103504295 PE=4 SV=1)

HSP 1 Score: 410.2 bits (1053), Expect = 3.7e-111
Identity = 205/245 (83.67%), Postives = 222/245 (90.61%), Query Frame = 0

Query: 26  PIPNVHPASSQFCPNLKFNPKRSLFVVFTSSQKPQFPCKSRRRSSVQCLFTGIVEEMGRV 85
           P+ N+H  SSQF PN  FN K SLF +FTS+ KPQ   +SR R+SVQCLFTGIVEE+G V
Sbjct: 4   PMGNLHLPSSQFFPNFNFNTKTSLFTLFTSTHKPQLCYRSRPRASVQCLFTGIVEEIGHV 63

Query: 86  KNLGIAENGGFDLEITAKTVLQGVNLGDSIAVNGTCLTVTAFDHKLSEFKVGLAPETLRK 145
           KNLGI ENGG+DLEI AKTVL+GVNLGDSIAVNGTCLTVTAFDH+LSEFKVGLAPETLRK
Sbjct: 64  KNLGIGENGGYDLEINAKTVLEGVNLGDSIAVNGTCLTVTAFDHQLSEFKVGLAPETLRK 123

Query: 146 TSLVELVPGSPVNLERAVQPISRMGGHFVQGHVDGTGEIVSMDAEGDSLWVKVRTSEAVL 205
           TSLVEL PGSPVNLERAVQPISRMGGHFVQGHVDGTGEI+SMD EGDSLW+KV+TSEA+L
Sbjct: 124 TSLVELSPGSPVNLERAVQPISRMGGHFVQGHVDGTGEIISMDPEGDSLWIKVKTSEALL 183

Query: 206 NYIVAKGFIAVDGTSLTVVDVFDEEKAFNFMLVAYTQNAVVVPLKKVGDLVNLEVDVLGK 265
           NYIV KGFIAVDGTSLTVVDVFD+EKAFNFMLVAYTQ  VV+PLKKVG LVNLEVD+LGK
Sbjct: 184 NYIVPKGFIAVDGTSLTVVDVFDDEKAFNFMLVAYTQKNVVIPLKKVGHLVNLEVDILGK 243

Query: 266 YVQRL 271
           YVQRL
Sbjct: 244 YVQRL 248

BLAST of Carg13586-RA vs. TrEMBL
Match: tr|M5VH27|M5VH27_PRUPE (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_3G137500 PE=4 SV=1)

HSP 1 Score: 353.2 bits (905), Expect = 5.4e-94
Identity = 182/272 (66.91%), Postives = 218/272 (80.15%), Query Frame = 0

Query: 19  MASPARNPIPNV---------HPASSQFCPNLKFNPKRSLFVVFTSSQKPQFPCKSRRRS 78
           ++ P+R P P+V         H  +S+   N     K S   +F S+Q+P    KS  R+
Sbjct: 8   LSLPSRTPEPSVRKMSILNKFHSVASRTHCNFDAIFKPSTLTLFISTQRPHAHIKSPPRN 67

Query: 79  SVQCLFTGIVEEMGRVKNLGIAENGGFDLEITAKTVLQGVNLGDSIAVNGTCLTVTAFDH 138
           +++CLFTGIVEEMG++K LG AENGGFD++I AKTVL+GV+LGDSIAVNGTCLTVT FD 
Sbjct: 68  TIRCLFTGIVEEMGQIKQLGTAENGGFDMKIGAKTVLEGVHLGDSIAVNGTCLTVTEFDT 127

Query: 139 KLSEFKVGLAPETLRKTSLVELVPGSPVNLERAVQPISRMGGHFVQGHVDGTGEIVSMDA 198
           +LS+F VGL+PETLRKTSL+EL PGS VNLERAVQP SRMGGHFVQGHVDGTGEIVSMD 
Sbjct: 128 QLSDFTVGLSPETLRKTSLIELEPGSLVNLERAVQPTSRMGGHFVQGHVDGTGEIVSMDP 187

Query: 199 EGDSLWVKVRTSEAVLNYIVAKGFIAVDGTSLTVVDVFDEEKAFNFMLVAYTQNAVVVPL 258
           EGDSLW+KV+ S+ +L Y+V KGFIAVDGTSLTVVDVFD+E +FNFMLVAYTQ  VV+PL
Sbjct: 188 EGDSLWIKVKASKELLKYVVPKGFIAVDGTSLTVVDVFDKEDSFNFMLVAYTQQNVVIPL 247

Query: 259 KKVGDLVNLEVDVLGKYVQRLLSNGAIGSIES 282
           KKVG  VNLEVD+LGKYV+RLLS+G + SI+S
Sbjct: 248 KKVGQKVNLEVDILGKYVERLLSSGFVESIKS 279

BLAST of Carg13586-RA vs. TrEMBL
Match: tr|A0A2P4LYB6|A0A2P4LYB6_QUESU (Riboflavin synthase OS=Quercus suber OX=58331 GN=CFP56_04658 PE=4 SV=1)

HSP 1 Score: 345.1 bits (884), Expect = 1.5e-91
Identity = 187/281 (66.55%), Postives = 217/281 (77.22%), Query Frame = 0

Query: 6   SLSAATALSSIPKMASPARNPI--PNVHPASSQFCPNLKFNP--KRSLFVVFTSSQK-PQ 65
           S  + T+ S IPK   P    +  P + P      P+LKFNP  K S   +F   QK P+
Sbjct: 4   SCFSLTSFSKIPKPTFPKAAILTPPAISPK-----PHLKFNPIFKPSTLSLFLPIQKNPK 63

Query: 66  FPCKSRRRSSVQCLFTGIVEEMGRVKNLGIAENGGFDLEITAKTVLQGVNLGDSIAVNGT 125
              ++R  S + CLFTGIVEE+G +K LGIAE+GGFD+ I AKTVL+GVNLGDSIAVNGT
Sbjct: 64  THFRTRFESHICCLFTGIVEEVGEIKQLGIAEDGGFDMRIAAKTVLEGVNLGDSIAVNGT 123

Query: 126 CLTVTAFDHKLSEFKVGLAPETLRKTSLVELVPGSPVNLERAVQPISRMGGHFVQGHVDG 185
           CLTV  FD +LSEF VGLAPETLRKTSL+EL PGS VNLERAVQP SRMGGHFVQGHVDG
Sbjct: 124 CLTVAEFDTQLSEFTVGLAPETLRKTSLIELGPGSLVNLERAVQPTSRMGGHFVQGHVDG 183

Query: 186 TGEIVSMDAEGDSLWVKVRTSEAVLNYIVAKGFIAVDGTSLTVVDVFDEEKAFNFMLVAY 245
           TGEIVS + EGDSLW+KV+T + +L YIV KGFIAVDGTSLTVVDVFD+E+ FNFMLVAY
Sbjct: 184 TGEIVSKEPEGDSLWIKVKTGKELLKYIVPKGFIAVDGTSLTVVDVFDDEECFNFMLVAY 243

Query: 246 TQNAVVVPLKKVGDLVNLEVDVLGKYVQRLLSNGAIGSIES 282
           TQ  VV+PLK VG  VNLEVD+LGKYV+RLLS+G + SI+S
Sbjct: 244 TQEKVVIPLKNVGQKVNLEVDILGKYVERLLSSGFVDSIKS 279

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022947746.12.5e-15199.65riboflavin synthase-like [Cucurbita moschata][more]
XP_023532107.11.6e-15098.23riboflavin synthase-like [Cucurbita pepo subsp. pepo][more]
XP_022970720.13.1e-14697.15riboflavin synthase-like [Cucurbita maxima][more]
XP_022140671.11.2e-12482.92LOW QUALITY PROTEIN: riboflavin synthase [Momordica charantia] >AFZ99647.1 ribof... [more]
XP_022986014.15.8e-12482.21riboflavin synthase-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT2G20690.13.3e-8170.81lumazine-binding family protein[more]
Match NameE-valueIdentityDescription
sp|Q9Y7P0|RISA_SCHPO8.7e-4750.75Riboflavin synthase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=28... [more]
sp|A5DB51|RISA_PICGU1.6e-4547.47Riboflavin synthase OS=Meyerozyma guilliermondii (strain ATCC 6260 / CBS 566 / D... [more]
sp|P50854|RISA_ACTPL1.7e-4246.73Riboflavin synthase OS=Actinobacillus pleuropneumoniae OX=715 GN=ribE PE=3 SV=1[more]
sp|P38145|RISA_YEAST1.6e-4046.60Riboflavin synthase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=... [more]
sp|O67604|RISA_AQUAE5.3e-3643.72Riboflavin synthase OS=Aquifex aeolicus (strain VF5) OX=224324 GN=ribE PE=3 SV=1[more]
Match NameE-valueIdentityDescription
tr|L0C7R1|L0C7R1_MOMCH7.7e-12582.92Riboflavin synthase OS=Momordica charantia OX=3673 PE=2 SV=1[more]
tr|A0A0A0KAI5|A0A0A0KAI5_CUCSA3.1e-11879.72Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G128550 PE=4 SV=1[more]
tr|A0A1S3CSC8|A0A1S3CSC8_CUCME3.7e-11183.67riboflavin synthase OS=Cucumis melo OX=3656 GN=LOC103504295 PE=4 SV=1[more]
tr|M5VH27|M5VH27_PRUPE5.4e-9466.91Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_3G137500 PE=4 SV=1[more]
tr|A0A2P4LYB6|A0A2P4LYB6_QUESU1.5e-9166.55Riboflavin synthase OS=Quercus suber OX=58331 GN=CFP56_04658 PE=4 SV=1[more]
The following terms have been associated with this mRNA:
Vocabulary: INTERPRO
TermDefinition
IPR017938Riboflavin_synthase-like_b-brl
IPR026017Lumazine-bd_dom
IPR023366ATP_synth_asu-like_sf
IPR001783Lumazine-bd
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0003674 molecular_function

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Carg13586Carg13586gene


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg13586-RA.CDS.1Carg13586-RA.CDS.1CDS


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg13586-RA.exon.1Carg13586-RA.exon.1exon


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Carg13586-RACarg13586-RA-proteinpolypeptide


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001783Lumazine-binding proteinTIGRFAMTIGR00187TIGR00187coord: 74..274
e-value: 3.0E-60
score: 200.8
IPR001783Lumazine-binding proteinPANTHERPTHR21098RIBOFLAVIN SYNTHASE ALPHA CHAINcoord: 68..274
IPR001783Lumazine-binding proteinCDDcd00402Riboflavin_synthase_likecoord: 75..263
e-value: 2.1455E-84
score: 252.694
IPR023366ATP synthase subunit alpha, N-terminal domain-like superfamilyGENE3DG3DSA:2.40.30.20coord: 177..278
e-value: 3.3E-31
score: 108.8
IPR023366ATP synthase subunit alpha, N-terminal domain-like superfamilyGENE3DG3DSA:2.40.30.20coord: 74..164
e-value: 9.3E-29
score: 101.2
IPR026017Lumazine-binding domainPFAMPF00677Lum_bindingcoord: 175..261
e-value: 5.3E-20
score: 71.1
coord: 76..162
e-value: 2.9E-21
score: 75.1
IPR026017Lumazine-binding domainPROSITEPS51177LUMAZINE_BINDcoord: 173..271
score: 22.015
IPR026017Lumazine-binding domainPROSITEPS51177LUMAZINE_BINDcoord: 74..172
score: 24.576
NoneNo IPR availablePANTHERPTHR21098:SF0RIBOFLAVIN SYNTHASEcoord: 68..274
IPR017938Riboflavin synthase-like beta-barrelSUPERFAMILYSSF63380Riboflavin synthase domain-likecoord: 168..272
IPR017938Riboflavin synthase-like beta-barrelSUPERFAMILYSSF63380Riboflavin synthase domain-likecoord: 74..164