Carg12385-RA (mRNA) Silver-seed gourd

NameCarg12385-RA
TypemRNA
OrganismCucurbita argyrosperma (Silver-seed gourd)
Descriptionabscisic acid 8'-hydroxylase 2
LocationCucurbita_argyrosperma_scaffold_074 : 679065 .. 681055 (+)
Sequence length462
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGAGAATTGCCCTGTGATCGTGATCAAGGCTTCAGCCCCTGGCGACAAAAGTAGTCGATATTGGCCAGCTGTAAGAACAATCATAATTTTTTTTTCTTTCTCTAGCGCCATTCACCAAACCATTTCTTCCTTCTCCACGTTTCAACATTTTACACCTTATGTTTTTCTTTTGCTGATCTAGCCGCCAAGAGGGGTAGGAGGTTTGATTCTTTGTTTTTTCTGGGGTTTACGTTAATTGATGAAATATGATGTTTCTTTGCACAACTGTTTGTGAAAATGTCACAATTGAATTATGATGTTTTCTGGGTCACGCCTGTGAAGTGTTTGTGAAAATGTCACTGTTGAATTTATGGTGTGTTTGTGGCAGTGTCCGACTTAATTAGTCGTTGGCCTAATCTGTTTGCGAAGTGTTTGTGAAAATATCTGACTGAATTATGAATTATGATCGTAGTTTGCCTACGAAGTGTTTGTGGAAATGTCAGAGTTGAATTGTGATGTTTCTGTGTGATGCCTGCAAACTGTTTGTGATAATGTCTGTTTGAGATAGTTAGCTATTTGGCTAATCTTGGCTGTGTGCTTTTGTTTTGGGGGATCAGATGGAAAGTGGTGGGAGAGGAATCTGGGATACAATACGGTCCATTTCCTGTACCTAAAGGTGGCTTACCCATTAAAGTATTTTCAAGGAAGAACAAGATTACTTCTTTGAATTGATGCGGATTCTCAACGACAAATTGGAGCTGAACTTCAAGTCCTTTTTCATCTGTAACACCATTTTCCAATTGTCAATGGAAATCCTTTTGCATTACTTTCTGTACTGCCTTTTTTGTCTGGTTTGCCATAATTGTCAAAGCAGTTATGTTCATTGAGTCTCAAACTTCAATTTGTTTATATTCAAAGAGGAAAAAGAAAGAGAGAAAAAAGAAAAACCTCATCTGATATGTGAATGTGAGAGAAAGTTTCGAGGGCTTTGAGGATGAAATGTGAGTGGTCAGATTCATGGATAAGGTTGCTTTCATTTTGGCTGATGGATGGATTTCTGAATCCCCACTGGGAGCCCCCCTCTGTGAAGTGTGAAAGGACAAGGACAAGCTTGGCCTCGCTGGAATCAGACCCACGAGGTAATAATAATAATCAGACCCAAATGGTGTGTGGGTTTGAGACATATAACATCAGTACGTTGTGCACCCTAGAATTCCACGTTATGCTGTGTCAGGACACCAAAATTGCAGCCCTACGTGGATCGTTAACCATGACCAATTCATCTCAACACTGGAGGGATTTTGGAAGTTCAGTCTTTATGTCATTTGTATTTGAGATGAGGGAGACAATATTTGTTCATGAGCTTGTTCTTATTCTGTTAAGCTTCTTTTTTCTATTACATTTCCCTCTAAACCCATTTCTGTTCATTTGTAACATTAAACCATATTGCAATAACACTAGCACAAGTAGTATGCTTTGCTTTGATGCAGTCCCTATGATTTTGATCCTTACCGCTTCATTGAAGTTTCAATTTGATTGATCATACGTAGAACTATTTAGTCTCTGAACTTTCTCGAGTAGTTGATATCTTTCGAGAACTTCAAAAAGGTATGAAGCTCAAAGACAGGTATCATAAATTGGTTTCAATAATTTGAATGGTATAAAGCAACTCCAAATCCGATGATTGATCCTACTCAAGTAGATTACATAAGGTACAATTCGCTGAAAATGTCATCTACAGTACAATAATTTAAGACTGCAAGGGTCAAGAAATGAATGCTGTGATGCAATGGCCCCTACAAAGAAATCATAACTCACAAGGAAATTTGAAAGTTCATATGACTTATGCATCATTCATTCATTGATAGGCTAGCAGTGGCAGAGGCAGCAGCAGCAAACTTTTGGATTTACCATAGCCACGGCCAGCCAGCCAGCTATCCTGAAATGATTGAACATCTCATCCTTCAAAACAGTGAATCTATCAGCACACAATCTTACAGAGCATGA

mRNA sequence

ATGGCGGAGAATTGCCCTGTGATCGTGATCAAGGCTTCAGCCCCTGGCGACAAAAGTAGTCGATATTGGCCAGCTCGCCATTCACCAAACCATTTCTTCCTTCTCCACGTTTCAACATTTTACACCTTATGTTTTTCTTTTGCTGATCTAGCCGCCAAGAGGGGTAGGAGATGGAAAGTGGTGGGAGAGGAATCTGGGATACAATACGGTCCATTTCCTGTACCTAAAGGTGGCTTACCCATTAAAAACTATTTAGTCTCTGAACTTTCTCGAGTAGTTGATATCTTTCGAGAACTTCAAAAAGGTATGAAGCTCAAAGACAGGCTAGCAGTGGCAGAGGCAGCAGCAGCAAACTTTTGGATTTACCATAGCCACGGCCAGCCAGCCAGCTATCCTGAAATGATTGAACATCTCATCCTTCAAAACAGTGAATCTATCAGCACACAATCTTACAGAGCATGA

Coding sequence (CDS)

ATGGCGGAGAATTGCCCTGTGATCGTGATCAAGGCTTCAGCCCCTGGCGACAAAAGTAGTCGATATTGGCCAGCTCGCCATTCACCAAACCATTTCTTCCTTCTCCACGTTTCAACATTTTACACCTTATGTTTTTCTTTTGCTGATCTAGCCGCCAAGAGGGGTAGGAGATGGAAAGTGGTGGGAGAGGAATCTGGGATACAATACGGTCCATTTCCTGTACCTAAAGGTGGCTTACCCATTAAAAACTATTTAGTCTCTGAACTTTCTCGAGTAGTTGATATCTTTCGAGAACTTCAAAAAGGTATGAAGCTCAAAGACAGGCTAGCAGTGGCAGAGGCAGCAGCAGCAAACTTTTGGATTTACCATAGCCACGGCCAGCCAGCCAGCTATCCTGAAATGATTGAACATCTCATCCTTCAAAACAGTGAATCTATCAGCACACAATCTTACAGAGCATGA

Protein sequence

MAENCPVIVIKASAPGDKSSRYWPARHSPNHFFLLHVSTFYTLCFSFADLAAKRGRRWKVVGEESGIQYGPFPVPKGGLPIKNYLVSELSRVVDIFRELQKGMKLKDRLAVAEAAAANFWIYHSHGQPASYPEMIEHLILQNSESISTQSYRA
BLAST of Carg12385-RA vs. NCBI nr
Match: XP_022948432.1 (abscisic acid 8'-hydroxylase 2-like [Cucurbita moschata])

HSP 1 Score: 63.2 bits (152), Expect = 9.1e-07
Identity = 27/28 (96.43%), Postives = 27/28 (96.43%), Query Frame = 0

Query: 55  GRRWKVVGEESGIQYGPFPVPKGGLPIK 83
           G RWKVVGEESGIQYGPFPVPKGGLPIK
Sbjct: 433 GYRWKVVGEESGIQYGPFPVPKGGLPIK 460

BLAST of Carg12385-RA vs. NCBI nr
Match: XP_023523016.1 (abscisic acid 8'-hydroxylase 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 63.2 bits (152), Expect = 9.1e-07
Identity = 27/28 (96.43%), Postives = 27/28 (96.43%), Query Frame = 0

Query: 55  GRRWKVVGEESGIQYGPFPVPKGGLPIK 83
           G RWKVVGEESGIQYGPFPVPKGGLPIK
Sbjct: 433 GYRWKVVGEESGIQYGPFPVPKGGLPIK 460

BLAST of Carg12385-RA vs. NCBI nr
Match: XP_022997684.1 (abscisic acid 8'-hydroxylase 2-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 61.6 bits (148), Expect = 2.6e-06
Identity = 26/26 (100.00%), Postives = 26/26 (100.00%), Query Frame = 0

Query: 57  RWKVVGEESGIQYGPFPVPKGGLPIK 83
           RWKVVGEESGIQYGPFPVPKGGLPIK
Sbjct: 440 RWKVVGEESGIQYGPFPVPKGGLPIK 465

BLAST of Carg12385-RA vs. NCBI nr
Match: XP_022997682.1 (abscisic acid 8'-hydroxylase 2-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 61.6 bits (148), Expect = 2.6e-06
Identity = 26/26 (100.00%), Postives = 26/26 (100.00%), Query Frame = 0

Query: 57  RWKVVGEESGIQYGPFPVPKGGLPIK 83
           RWKVVGEESGIQYGPFPVPKGGLPIK
Sbjct: 440 RWKVVGEESGIQYGPFPVPKGGLPIK 465

BLAST of Carg12385-RA vs. NCBI nr
Match: XP_011658248.1 (PREDICTED: abscisic acid 8'-hydroxylase 2 isoform X1 [Cucumis sativus])

HSP 1 Score: 60.1 bits (144), Expect = 7.7e-06
Identity = 26/28 (92.86%), Postives = 26/28 (92.86%), Query Frame = 0

Query: 57  RWKVVGEESGIQYGPFPVPKGGLPIKNY 85
           RWKVVGEES IQYGPFPVPKGGLPIK Y
Sbjct: 433 RWKVVGEESEIQYGPFPVPKGGLPIKVY 460

BLAST of Carg12385-RA vs. TAIR10
Match: AT2G29090.1 (cytochrome P450, family 707, subfamily A, polypeptide 2)

HSP 1 Score: 52.8 bits (125), Expect = 2.2e-07
Identity = 20/26 (76.92%), Postives = 24/26 (92.31%), Query Frame = 0

Query: 57  RWKVVGEESGIQYGPFPVPKGGLPIK 83
           RW+V+G+E GIQYGPFPVPK GLPI+
Sbjct: 453 RWEVIGDEEGIQYGPFPVPKKGLPIR 478

BLAST of Carg12385-RA vs. TAIR10
Match: AT3G19270.1 (cytochrome P450, family 707, subfamily A, polypeptide 4)

HSP 1 Score: 43.1 bits (100), Expect = 1.8e-04
Identity = 16/24 (66.67%), Postives = 19/24 (79.17%), Query Frame = 0

Query: 57  RWKVVGEESGIQYGPFPVPKGGLP 81
           RW+V G E GIQY PFP+P+ GLP
Sbjct: 437 RWEVKGGEKGIQYSPFPIPQNGLP 460

BLAST of Carg12385-RA vs. TAIR10
Match: AT5G45340.1 (cytochrome P450, family 707, subfamily A, polypeptide 3)

HSP 1 Score: 43.1 bits (100), Expect = 1.8e-04
Identity = 16/25 (64.00%), Postives = 19/25 (76.00%), Query Frame = 0

Query: 57  RWKVVGEESGIQYGPFPVPKGGLPI 82
           RW +VG   GIQYGPF +P+ GLPI
Sbjct: 433 RWSIVGPSDGIQYGPFALPQNGLPI 457

BLAST of Carg12385-RA vs. Swiss-Prot
Match: sp|O81077|ABAH2_ARATH (Abscisic acid 8'-hydroxylase 2 OS=Arabidopsis thaliana OX=3702 GN=CYP707A2 PE=2 SV=1)

HSP 1 Score: 52.8 bits (125), Expect = 4.0e-06
Identity = 20/26 (76.92%), Postives = 24/26 (92.31%), Query Frame = 0

Query: 57  RWKVVGEESGIQYGPFPVPKGGLPIK 83
           RW+V+G+E GIQYGPFPVPK GLPI+
Sbjct: 453 RWEVIGDEEGIQYGPFPVPKKGLPIR 478

BLAST of Carg12385-RA vs. TrEMBL
Match: tr|A0A1S3C5P5|A0A1S3C5P5_CUCME (abscisic acid 8'-hydroxylase 2 OS=Cucumis melo OX=3656 GN=LOC103497144 PE=3 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 5.1e-06
Identity = 26/28 (92.86%), Postives = 26/28 (92.86%), Query Frame = 0

Query: 57  RWKVVGEESGIQYGPFPVPKGGLPIKNY 85
           RWKVVGEES IQYGPFPVPKGGLPIK Y
Sbjct: 433 RWKVVGEESEIQYGPFPVPKGGLPIKVY 460

BLAST of Carg12385-RA vs. TrEMBL
Match: tr|A0A2H4X2V1|A0A2H4X2V1_CAMSI (ABA 8'-hydroxylase OS=Camellia sinensis OX=4442 GN=CYP707A-2 PE=2 SV=1)

HSP 1 Score: 56.6 bits (135), Expect = 5.6e-05
Identity = 20/28 (71.43%), Postives = 26/28 (92.86%), Query Frame = 0

Query: 57  RWKVVGEESGIQYGPFPVPKGGLPIKNY 85
           RW+V+G+E G+QYGPFPVPKGGLP++ Y
Sbjct: 446 RWEVIGDEDGVQYGPFPVPKGGLPVRVY 473

BLAST of Carg12385-RA vs. TrEMBL
Match: tr|A0A220NVF6|A0A220NVF6_CAMSI (Abscisic acid 8'-hydroxylase 2 OS=Camellia sinensis OX=4442 PE=2 SV=1)

HSP 1 Score: 56.6 bits (135), Expect = 5.6e-05
Identity = 20/28 (71.43%), Postives = 26/28 (92.86%), Query Frame = 0

Query: 57  RWKVVGEESGIQYGPFPVPKGGLPIKNY 85
           RW+V+G+E G+QYGPFPVPKGGLP++ Y
Sbjct: 446 RWEVIGDEDGVQYGPFPVPKGGLPVRVY 473

BLAST of Carg12385-RA vs. TrEMBL
Match: tr|A0A2U1MHG3|A0A2U1MHG3_ARTAN (Cytochrome P450 OS=Artemisia annua OX=35608 GN=CTI12_AA380400 PE=4 SV=1)

HSP 1 Score: 55.8 bits (133), Expect = 9.6e-05
Identity = 23/32 (71.88%), Postives = 27/32 (84.38%), Query Frame = 0

Query: 57  RWKVVGEESGIQYGPFPVPKGGLPIKNYLVSE 89
           +W+VVGE  GIQYGPFPVPK GLPIK + +SE
Sbjct: 446 KWEVVGEADGIQYGPFPVPKQGLPIKLHRISE 477

BLAST of Carg12385-RA vs. TrEMBL
Match: tr|A0A1U7ZTJ9|A0A1U7ZTJ9_NELNU (abscisic acid 8'-hydroxylase 2-like OS=Nelumbo nucifera OX=4432 GN=LOC104597161 PE=3 SV=1)

HSP 1 Score: 55.8 bits (133), Expect = 9.6e-05
Identity = 21/26 (80.77%), Postives = 25/26 (96.15%), Query Frame = 0

Query: 57  RWKVVGEESGIQYGPFPVPKGGLPIK 83
           RWKVVGE+ GI+YGPFPVPKGGLP++
Sbjct: 446 RWKVVGEDDGIKYGPFPVPKGGLPVR 471

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022948432.19.1e-0796.43abscisic acid 8'-hydroxylase 2-like [Cucurbita moschata][more]
XP_023523016.19.1e-0796.43abscisic acid 8'-hydroxylase 2 [Cucurbita pepo subsp. pepo][more]
XP_022997684.12.6e-06100.00abscisic acid 8'-hydroxylase 2-like isoform X2 [Cucurbita maxima][more]
XP_022997682.12.6e-06100.00abscisic acid 8'-hydroxylase 2-like isoform X1 [Cucurbita maxima][more]
XP_011658248.17.7e-0692.86PREDICTED: abscisic acid 8'-hydroxylase 2 isoform X1 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
AT2G29090.12.2e-0776.92cytochrome P450, family 707, subfamily A, polypeptide 2[more]
AT3G19270.11.8e-0466.67cytochrome P450, family 707, subfamily A, polypeptide 4[more]
AT5G45340.11.8e-0464.00cytochrome P450, family 707, subfamily A, polypeptide 3[more]
Match NameE-valueIdentityDescription
sp|O81077|ABAH2_ARATH4.0e-0676.92Abscisic acid 8'-hydroxylase 2 OS=Arabidopsis thaliana OX=3702 GN=CYP707A2 PE=2 ... [more]
Match NameE-valueIdentityDescription
tr|A0A1S3C5P5|A0A1S3C5P5_CUCME5.1e-0692.86abscisic acid 8'-hydroxylase 2 OS=Cucumis melo OX=3656 GN=LOC103497144 PE=3 SV=1[more]
tr|A0A2H4X2V1|A0A2H4X2V1_CAMSI5.6e-0571.43ABA 8'-hydroxylase OS=Camellia sinensis OX=4442 GN=CYP707A-2 PE=2 SV=1[more]
tr|A0A220NVF6|A0A220NVF6_CAMSI5.6e-0571.43Abscisic acid 8'-hydroxylase 2 OS=Camellia sinensis OX=4442 PE=2 SV=1[more]
tr|A0A2U1MHG3|A0A2U1MHG3_ARTAN9.6e-0571.88Cytochrome P450 OS=Artemisia annua OX=35608 GN=CTI12_AA380400 PE=4 SV=1[more]
tr|A0A1U7ZTJ9|A0A1U7ZTJ9_NELNU9.6e-0580.77abscisic acid 8'-hydroxylase 2-like OS=Nelumbo nucifera OX=4432 GN=LOC104597161 ... [more]
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0003674 molecular_function

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Carg12385Carg12385gene


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Carg12385-RACarg12385-RA-proteinpolypeptide


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg12385-RA.exon.1Carg12385-RA.exon.1exon
Carg12385-RA.exon.2Carg12385-RA.exon.2exon
Carg12385-RA.exon.3Carg12385-RA.exon.3exon
Carg12385-RA.exon.4Carg12385-RA.exon.4exon
Carg12385-RA.exon.5Carg12385-RA.exon.5exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg12385-RA.CDS.1Carg12385-RA.CDS.1CDS
Carg12385-RA.CDS.2Carg12385-RA.CDS.2CDS
Carg12385-RA.CDS.3Carg12385-RA.CDS.3CDS
Carg12385-RA.CDS.4Carg12385-RA.CDS.4CDS
Carg12385-RA.CDS.5Carg12385-RA.CDS.5CDS