Carg12372-RA (mRNA) Silver-seed gourd

NameCarg12372-RA
TypemRNA
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionGlucan endo-1,3-beta-glucosidase
LocationCucurbita_argyrosperma_scaffold_074 : 616253 .. 618721 (-)
Sequence length1846
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexonthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTAATTCTCTACTTCTCTCTCTCCTTCTTTTCTCTCTCTTCCTCCGCCTCTCCTTCGCCACCTACTCTATCGGTGTCAACTACGGCACCGTCGCCGACAATCTCCCTCCGCCCTCCCAGGTCGCCGCCTTCCTCAAATCCCACACCACCATCGACCGCGTCAAGATCTTTGACGCCAACCCCGATATTCTCCGTGCCTTCGCCGCTACTGGCATCGCCCTCACAGTCTCTGTCGCTAATGGCGACATTCCGGCCCTCGCCAAGCTCCCCGCCGCTCAATCCTGGATTGCCAATAACATTCTCCCGTTTCACCCTAACACTCTCATCAACCACATCGCCGTCGGGAACGAGATCCTCGCCACCTCCGACAAGAATCTCATAGCTCATATGCTCCCCGCGATGAAGGCTTTACATTCCGCTTTAACCCTCGCAAATATTACTGATATCAAGGTTTCGTCTCCTCATTCTTTGGGGATTTTGTCGGCATCGGAGCCGCCGAGTACCGGGCGGTTTCGGAAGGGGTACGATCGAGCCATATTCGCTCCGATCCTCGAGTTTCATCGCAAGACAAAGACGCCGTTCATGGTGAATCCGTATCCATTCTTTGGATTCAGACCGGCGACGTTGGACTACGCGCTGTTCAAGCCGAACGCCGGATTTTTCGACAATACCACCGGAAAACACTACACGAATATGTTCGATGCGCAGATGGACGCGGTTCACGCAGCGATGAACAAGCTCGGGTACGGGGATGTGGACATCGTCGTGGCGGAGACTGGGTGGCCATCCGCCGGTGACCCGAACCAACCCGGCGTGAGCTTGGAGAACGCTATTTCTTACAACCAGAACCTCGTGAAGCACGTGAACTCCGGTAAGGGGACGCCAATGATGCCCAACAGAACTTTTGAAACCTACGTCTTCTCTCTTTTCAACGAGAATCTCAAACCGTCGGTCTCGGAGCGGAACTACGGTCTGTTCAAGCCCGATCTAACTCCGGTCTACGACGTCGGCATCCTCCGAAACTCACCGGTAAACCCCTTCTTCATCTATTTAAATCCCTTGGCCTCTGCCGGAATTATGCCAAACCCGGTGGGCGCATGTTAGTAAATTAAGTTGTTATCCAGGCACGCCAAATTACTGGTCTGCGGACCAGTGGAAGAAGAGTATTACACGGAAACCCCTCTTCCCATGAGTTCGATAACACGTGTTGCTCCCTAATTGGTCCCTAAGTATCACCTGTAAATTTGTCGTTTTACTAACCAATAAAAAACTGACGCGTGGGTGTACGAGTTTCTGACAGCAAGTCCATAGTGCGGTCCTGCTGTCTAAGGAGGCAAAAAGCCACCTGTCCTAATTTTTTTTCTGACGGAATTACCCTCCATTTAACTTCCTAATTCCATGGCATTAAAGTAAATACGCTTTTTTTTCTTTTTCTTTTGCCTTTTTTGAGTGAATTCCCGCACCACATTTATTCCAGATGATATTGGATTGTGACCGTTGATTTTTATGTAACAGACAACGGGCCCCACAGCACCGGCAGGGCAGGCGCCGCAGCCGTCGGATGGTGGGAGGAAGTGGTGCGTGCCGAAAACGGACGCGTCAGATGAAGCGTTGCAGAAGAATATTGACTACGTGTGTAGCAGTGGAGTGGACTGTGGGCCCATACAAGCAGGTGGGGCCTGCTACGATCCGAATACAGTGCGCTCACATGCATCCTACGCCATGGACGCTTACTTTCAGTCCTCTGGTCGCCATGATTTCAACTGCGATTTCAATCACACTGGCGTCCTCACTTCCACCGACCCCAGTAAGATACTTTATTTAATCATATAGTTTTTAATTTAATTATTTATCATTTCAATTCTTACAATAAAATTAATAGATCTTAGGTTAAATTATATTAATAGATTTGTGGTAGTACCTAAGATATTATTTTGAGATCTATTGGATGATAGTGTATAGCTTAGAGACCCGTTATGTAAATATTATTTTGGGATTGTACAGGTTACAAAGCCTGCAGTTACCCGTTGGGTGCCGAAAAGTTAACGGAGAAATCGGTGGCCTCTGCGGCGGCGATGAGGCGTAGGATCAGGTTGTTGGGGGCGGTGGCGCGGACATGTATTGCGGTGGTAGCTGGCGGTGTATTATTAATTTGAACCCAAAAAGTGTAATTTATGACCGTTGGAGTTGGGGCTCTTGGAGTTCATAGGAATTTTAGATGTAGTAATTAATGCTATTAGTTTTATATTTAATTCTGAATGCTTTTTGTCACTTTTTGACCATCTAATTGACTTGAATTTTTTTTGCAGATTGATTTTTTTTTTTTATCAATGCGACTAATTCTAATTACAATCTATCGTACCCTACTTTTAGAATTCAAATTCAATACTGGTTAAGAATATTTAATACTAAATTATAGTTTATCATATATATCATATCTGATATTTTATTATAAGGGAAATATCT

mRNA sequence

ATGGCTAATTCTCTACTTCTCTCTCTCCTTCTTTTCTCTCTCTTCCTCCGCCTCTCCTTCGCCACCTACTCTATCGGTGTCAACTACGGCACCGTCGCCGACAATCTCCCTCCGCCCTCCCAGGTCGCCGCCTTCCTCAAATCCCACACCACCATCGACCGCGTCAAGATCTTTGACGCCAACCCCGATATTCTCCGTGCCTTCGCCGCTACTGGCATCGCCCTCACAGTCTCTGTCGCTAATGGCGACATTCCGGCCCTCGCCAAGCTCCCCGCCGCTCAATCCTGGATTGCCAATAACATTCTCCCGTTTCACCCTAACACTCTCATCAACCACATCGCCGTCGGGAACGAGATCCTCGCCACCTCCGACAAGAATCTCATAGCTCATATGCTCCCCGCGATGAAGGCTTTACATTCCGCTTTAACCCTCGCAAATATTACTGATATCAAGGTTTCGTCTCCTCATTCTTTGGGGATTTTGTCGGCATCGGAGCCGCCGAGTACCGGGCGGTTTCGGAAGGGGTACGATCGAGCCATATTCGCTCCGATCCTCGAGTTTCATCGCAAGACAAAGACGCCGTTCATGGTGAATCCGTATCCATTCTTTGGATTCAGACCGGCGACGTTGGACTACGCGCTGTTCAAGCCGAACGCCGGATTTTTCGACAATACCACCGGAAAACACTACACGAATATGTTCGATGCGCAGATGGACGCGGTTCACGCAGCGATGAACAAGCTCGGGTACGGGGATGTGGACATCGTCGTGGCGGAGACTGGGTGGCCATCCGCCGGTGACCCGAACCAACCCGGCGTGAGCTTGGAGAACGCTATTTCTTACAACCAGAACCTCGTGAAGCACGTGAACTCCGGTAAGGGGACGCCAATGATGCCCAACAGAACTTTTGAAACCTACGTCTTCTCTCTTTTCAACGAGAATCTCAAACCGTCGGTCTCGGAGCGGAACTACGGTCTGTTCAAGCCCGATCTAACTCCGGTCTACGACGTCGGCATCCTCCGAAACTCACCGGTAAACCCCTTCTTCATCTATTTAAATCCCTTGGCCTCTGCCGGAATTATGCCAAACCCGACAACGGGCCCCACAGCACCGGCAGGGCAGGCGCCGCAGCCGTCGGATGGTGGGAGGAAGTGGTGCGTGCCGAAAACGGACGCGTCAGATGAAGCGTTGCAGAAGAATATTGACTACGTGTGTAGCAGTGGAGTGGACTGTGGGCCCATACAAGCAGGTGGGGCCTGCTACGATCCGAATACAGTGCGCTCACATGCATCCTACGCCATGGACGCTTACTTTCAGTCCTCTGGTCGCCATGATTTCAACTGCGATTTCAATCACACTGGCGTCCTCACTTCCACCGACCCCAGTTACAAAGCCTGCAGTTACCCGTTGGGTGCCGAAAAGTTAACGGAGAAATCGGTGGCCTCTGCGGCGGCGATGAGGCGTAGGATCAGGTTGTTGGGGGCGGTGGCGCGGACATGTATTGCGGTGGTAGCTGGCGGTGTATTATTAATTTGAACCCAAAAAGTGTAATTTATGACCGTTGGAGTTGGGGCTCTTGGAGTTCATAGGAATTTTAGATGTAGTAATTAATGCTATTAGTTTTATATTTAATTCTGAATGCTTTTTGTCACTTTTTGACCATCTAATTGACTTGAATTTTTTTTGCAGATTGATTTTTTTTTTTTATCAATGCGACTAATTCTAATTACAATCTATCGTACCCTACTTTTAGAATTCAAATTCAATACTGGTTAAGAATATTTAATACTAAATTATAGTTTATCATATATATCATATCTGATATTTTATTATAAGGGAAATATCT

Coding sequence (CDS)

ATGGCTAATTCTCTACTTCTCTCTCTCCTTCTTTTCTCTCTCTTCCTCCGCCTCTCCTTCGCCACCTACTCTATCGGTGTCAACTACGGCACCGTCGCCGACAATCTCCCTCCGCCCTCCCAGGTCGCCGCCTTCCTCAAATCCCACACCACCATCGACCGCGTCAAGATCTTTGACGCCAACCCCGATATTCTCCGTGCCTTCGCCGCTACTGGCATCGCCCTCACAGTCTCTGTCGCTAATGGCGACATTCCGGCCCTCGCCAAGCTCCCCGCCGCTCAATCCTGGATTGCCAATAACATTCTCCCGTTTCACCCTAACACTCTCATCAACCACATCGCCGTCGGGAACGAGATCCTCGCCACCTCCGACAAGAATCTCATAGCTCATATGCTCCCCGCGATGAAGGCTTTACATTCCGCTTTAACCCTCGCAAATATTACTGATATCAAGGTTTCGTCTCCTCATTCTTTGGGGATTTTGTCGGCATCGGAGCCGCCGAGTACCGGGCGGTTTCGGAAGGGGTACGATCGAGCCATATTCGCTCCGATCCTCGAGTTTCATCGCAAGACAAAGACGCCGTTCATGGTGAATCCGTATCCATTCTTTGGATTCAGACCGGCGACGTTGGACTACGCGCTGTTCAAGCCGAACGCCGGATTTTTCGACAATACCACCGGAAAACACTACACGAATATGTTCGATGCGCAGATGGACGCGGTTCACGCAGCGATGAACAAGCTCGGGTACGGGGATGTGGACATCGTCGTGGCGGAGACTGGGTGGCCATCCGCCGGTGACCCGAACCAACCCGGCGTGAGCTTGGAGAACGCTATTTCTTACAACCAGAACCTCGTGAAGCACGTGAACTCCGGTAAGGGGACGCCAATGATGCCCAACAGAACTTTTGAAACCTACGTCTTCTCTCTTTTCAACGAGAATCTCAAACCGTCGGTCTCGGAGCGGAACTACGGTCTGTTCAAGCCCGATCTAACTCCGGTCTACGACGTCGGCATCCTCCGAAACTCACCGGTAAACCCCTTCTTCATCTATTTAAATCCCTTGGCCTCTGCCGGAATTATGCCAAACCCGACAACGGGCCCCACAGCACCGGCAGGGCAGGCGCCGCAGCCGTCGGATGGTGGGAGGAAGTGGTGCGTGCCGAAAACGGACGCGTCAGATGAAGCGTTGCAGAAGAATATTGACTACGTGTGTAGCAGTGGAGTGGACTGTGGGCCCATACAAGCAGGTGGGGCCTGCTACGATCCGAATACAGTGCGCTCACATGCATCCTACGCCATGGACGCTTACTTTCAGTCCTCTGGTCGCCATGATTTCAACTGCGATTTCAATCACACTGGCGTCCTCACTTCCACCGACCCCAGTTACAAAGCCTGCAGTTACCCGTTGGGTGCCGAAAAGTTAACGGAGAAATCGGTGGCCTCTGCGGCGGCGATGAGGCGTAGGATCAGGTTGTTGGGGGCGGTGGCGCGGACATGTATTGCGGTGGTAGCTGGCGGTGTATTATTAATTTGA

Protein sequence

MANSLLLSLLLFSLFLRLSFATYSIGVNYGTVADNLPPPSQVAAFLKSHTTIDRVKIFDANPDILRAFAATGIALTVSVANGDIPALAKLPAAQSWIANNILPFHPNTLINHIAVGNEILATSDKNLIAHMLPAMKALHSALTLANITDIKVSSPHSLGILSASEPPSTGRFRKGYDRAIFAPILEFHRKTKTPFMVNPYPFFGFRPATLDYALFKPNAGFFDNTTGKHYTNMFDAQMDAVHAAMNKLGYGDVDIVVAETGWPSAGDPNQPGVSLENAISYNQNLVKHVNSGKGTPMMPNRTFETYVFSLFNENLKPSVSERNYGLFKPDLTPVYDVGILRNSPVNPFFIYLNPLASAGIMPNPTTGPTAPAGQAPQPSDGGRKWCVPKTDASDEALQKNIDYVCSSGVDCGPIQAGGACYDPNTVRSHASYAMDAYFQSSGRHDFNCDFNHTGVLTSTDPSYKACSYPLGAEKLTEKSVASAAAMRRRIRLLGAVARTCIAVVAGGVLLI
BLAST of Carg12372-RA vs. NCBI nr
Match: XP_022948604.1 (glucan endo-1,3-beta-glucosidase-like [Cucurbita moschata])

HSP 1 Score: 964.1 bits (2491), Expect = 1.8e-277
Identity = 490/511 (95.89%), Postives = 490/511 (95.89%), Query Frame = 0

Query: 1   MANSLLLSLLLFSLFLRLSFATYSIGVNYGTVADNLPPPSQVAAFLKSHTTIDRVKIFDA 60
           MANSLLLSLLLFSLFLRLS ATYSIGVNYGTVADNLPPPSQVAAFLKSHTTIDRVKIFDA
Sbjct: 1   MANSLLLSLLLFSLFLRLSSATYSIGVNYGTVADNLPPPSQVAAFLKSHTTIDRVKIFDA 60

Query: 61  NPDILRAFAATGIALTVSVANGDIPALAKLPAAQSWIANNILPFHPNTLINHIAVGNEIL 120
           NPDILRAFAATGIALTVSVANGDIPALAKLPAAQSWIANNILPFHPNTLINHIAVGNEIL
Sbjct: 61  NPDILRAFAATGIALTVSVANGDIPALAKLPAAQSWIANNILPFHPNTLINHIAVGNEIL 120

Query: 121 ATSDKNLIAHMLPAMKALHSALTLANITDIKVSSPHSLGILSASEPPSTGRFRKGYDRAI 180
           ATSDKNLIAHMLPAMKALHSALTLANITDIKVSSPHSLGILSASEPPSTGRFRKGYDRAI
Sbjct: 121 ATSDKNLIAHMLPAMKALHSALTLANITDIKVSSPHSLGILSASEPPSTGRFRKGYDRAI 180

Query: 181 FAPILEFHRKTKTPFMVNPYPFFGFRPATLDYALFKPNAGFFDNTTGKHYTNMFDAQMDA 240
           FAPILEFHRKTKTPFMVNPYPFFGFRPATLDYALFKPNAGFFDNTTGKHYTNMFDAQMDA
Sbjct: 181 FAPILEFHRKTKTPFMVNPYPFFGFRPATLDYALFKPNAGFFDNTTGKHYTNMFDAQMDA 240

Query: 241 VHAAMNKLGYGDVDIVVAETGWPSAGDPNQPGVSLENAISYNQNLVKHVNSGKGTPMMPN 300
           VHAAMNKLGYGDVDIVVAETGWPSAGDPNQPGVSLENAISYNQNLVKHVNSGKGTPMMPN
Sbjct: 241 VHAAMNKLGYGDVDIVVAETGWPSAGDPNQPGVSLENAISYNQNLVKHVNSGKGTPMMPN 300

Query: 301 RTFETYVFSLFNENLKPSVSERNYGLFKPDLTPVYDVGILRNSPVNPFFIYLNPLASAGI 360
           RTFETYVFSLFNENLKPSVSERNYGLFKPDLTPVYDVGILRNS                 
Sbjct: 301 RTFETYVFSLFNENLKPSVSERNYGLFKPDLTPVYDVGILRNS----------------- 360

Query: 361 MPNPTTGPTAPAGQAPQPSDGGRKWCVPKTDASDEALQKNIDYVCSSGVDCGPIQAGGAC 420
              PTTGPTAPAGQAPQPSDGGRKWCVPKTDASDEALQKNIDYVCSSGVDCGPIQAGGAC
Sbjct: 361 ---PTTGPTAPAGQAPQPSDGGRKWCVPKTDASDEALQKNIDYVCSSGVDCGPIQAGGAC 420

Query: 421 YDPNTVRSHASYAMDAYFQSSGRHDFNCDFNHTGVLTSTDPSYKACSYPLGAEKLTEKSV 480
           YDPNTVRSHASYAMDAYFQSSGRHDFNCDFNHTGVLTSTDPSYKACSYPLGAEKLTEKSV
Sbjct: 421 YDPNTVRSHASYAMDAYFQSSGRHDFNCDFNHTGVLTSTDPSYKACSYPLGAEKLTEKSV 480

Query: 481 ASAAAMRRRIRLLGAVARTCIAVVAGGVLLI 512
           ASAAAMRRRIRLLGAVARTCIAVVAGGVLLI
Sbjct: 481 ASAAAMRRRIRLLGAVARTCIAVVAGGVLLI 491

BLAST of Carg12372-RA vs. NCBI nr
Match: XP_023522990.1 (glucan endo-1,3-beta-glucosidase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 953.4 bits (2463), Expect = 3.2e-274
Identity = 484/511 (94.72%), Postives = 488/511 (95.50%), Query Frame = 0

Query: 1   MANSLLLSLLLFSLFLRLSFATYSIGVNYGTVADNLPPPSQVAAFLKSHTTIDRVKIFDA 60
           MANSLLLSLLLFSLFLRLS ATYSIGVNYGTVADNLPPPSQVAAFLKSHTTIDRVKIFDA
Sbjct: 1   MANSLLLSLLLFSLFLRLSSATYSIGVNYGTVADNLPPPSQVAAFLKSHTTIDRVKIFDA 60

Query: 61  NPDILRAFAATGIALTVSVANGDIPALAKLPAAQSWIANNILPFHPNTLINHIAVGNEIL 120
           NPDILRAFAATGIALTVSVANGDIP+LAKLPAAQSWIANNILPFHPNTLINHIAVGNEIL
Sbjct: 61  NPDILRAFAATGIALTVSVANGDIPSLAKLPAAQSWIANNILPFHPNTLINHIAVGNEIL 120

Query: 121 ATSDKNLIAHMLPAMKALHSALTLANITDIKVSSPHSLGILSASEPPSTGRFRKGYDRAI 180
           ATSDKNLIAHMLPAMKALHSALTLANITDIKVSSPHSLGILSASEPPSTGRFRKGYDRAI
Sbjct: 121 ATSDKNLIAHMLPAMKALHSALTLANITDIKVSSPHSLGILSASEPPSTGRFRKGYDRAI 180

Query: 181 FAPILEFHRKTKTPFMVNPYPFFGFRPATLDYALFKPNAGFFDNTTGKHYTNMFDAQMDA 240
           FAPILEFHRK+KTPFMVNPYPFFGFRPATLDYALFKPNAGFFDNTTGKHYTNMFDAQMDA
Sbjct: 181 FAPILEFHRKSKTPFMVNPYPFFGFRPATLDYALFKPNAGFFDNTTGKHYTNMFDAQMDA 240

Query: 241 VHAAMNKLGYGDVDIVVAETGWPSAGDPNQPGVSLENAISYNQNLVKHVNSGKGTPMMPN 300
           VHAAMNKLGYGDVDIVVAETGWPSAGDPNQPGVSLENAISYN+NLVKHVNSGKGTPMMPN
Sbjct: 241 VHAAMNKLGYGDVDIVVAETGWPSAGDPNQPGVSLENAISYNRNLVKHVNSGKGTPMMPN 300

Query: 301 RTFETYVFSLFNENLKPSVSERNYGLFKPDLTPVYDVGILRNSPVNPFFIYLNPLASAGI 360
           RTFETYVFSLFNENLKPSVSERNYGLFKPDLTPVYDVGILRNS                 
Sbjct: 301 RTFETYVFSLFNENLKPSVSERNYGLFKPDLTPVYDVGILRNS----------------- 360

Query: 361 MPNPTTGPTAPAGQAPQPSDGGRKWCVPKTDASDEALQKNIDYVCSSGVDCGPIQAGGAC 420
              PTTGPTAPAGQAPQPSDG RKWCVPKTDASDEALQKNIDYVCSSGVDCGPIQAGGAC
Sbjct: 361 ---PTTGPTAPAGQAPQPSDGVRKWCVPKTDASDEALQKNIDYVCSSGVDCGPIQAGGAC 420

Query: 421 YDPNTVRSHASYAMDAYFQSSGRHDFNCDFNHTGVLTSTDPSYKACSYPLGAEKLTEKSV 480
           YDPNTVRSHASYAMDAYFQSSGRHDFNCDFNHTGVLTSTDPSYKAC+YPLGAEKLTEKSV
Sbjct: 421 YDPNTVRSHASYAMDAYFQSSGRHDFNCDFNHTGVLTSTDPSYKACTYPLGAEKLTEKSV 480

Query: 481 ASAAAMRRRIRLLGAVARTCIAVVAGGVLLI 512
           ASAAAMRRRIRLLGAVAR CIAVVAGGVLLI
Sbjct: 481 ASAAAMRRRIRLLGAVARACIAVVAGGVLLI 491

BLAST of Carg12372-RA vs. NCBI nr
Match: XP_022998328.1 (glucan endo-1,3-beta-glucosidase-like [Cucurbita maxima])

HSP 1 Score: 951.8 bits (2459), Expect = 9.3e-274
Identity = 483/511 (94.52%), Postives = 486/511 (95.11%), Query Frame = 0

Query: 1   MANSLLLSLLLFSLFLRLSFATYSIGVNYGTVADNLPPPSQVAAFLKSHTTIDRVKIFDA 60
           MANSLLLSLLL SLFLRLS AT+SIGVNYGTVADNLPPPSQVAAFLKSHTTIDRVKIFDA
Sbjct: 1   MANSLLLSLLLVSLFLRLSSATFSIGVNYGTVADNLPPPSQVAAFLKSHTTIDRVKIFDA 60

Query: 61  NPDILRAFAATGIALTVSVANGDIPALAKLPAAQSWIANNILPFHPNTLINHIAVGNEIL 120
           NPDILRAFA TGIALTVSVANGDIPALAKLPAAQSWIANNILPFHPNTLINHIAVGNEIL
Sbjct: 61  NPDILRAFAGTGIALTVSVANGDIPALAKLPAAQSWIANNILPFHPNTLINHIAVGNEIL 120

Query: 121 ATSDKNLIAHMLPAMKALHSALTLANITDIKVSSPHSLGILSASEPPSTGRFRKGYDRAI 180
           ATSDKNLIAHMLPAMKALHSALTLANITDIKVSSPHSLGILSASEPPSTGRFRKGYDRAI
Sbjct: 121 ATSDKNLIAHMLPAMKALHSALTLANITDIKVSSPHSLGILSASEPPSTGRFRKGYDRAI 180

Query: 181 FAPILEFHRKTKTPFMVNPYPFFGFRPATLDYALFKPNAGFFDNTTGKHYTNMFDAQMDA 240
           FAPILEFHRKTKTPFMVNPYPFFGFRPATL+YALFKPNAGFFDNTTGKHYTNMFDAQMDA
Sbjct: 181 FAPILEFHRKTKTPFMVNPYPFFGFRPATLEYALFKPNAGFFDNTTGKHYTNMFDAQMDA 240

Query: 241 VHAAMNKLGYGDVDIVVAETGWPSAGDPNQPGVSLENAISYNQNLVKHVNSGKGTPMMPN 300
           VHAAMNKLGYGDVDIVVAETGWPSAGDPNQPGVSLENAISYN+NLVKHVNSGKGTPMMPN
Sbjct: 241 VHAAMNKLGYGDVDIVVAETGWPSAGDPNQPGVSLENAISYNRNLVKHVNSGKGTPMMPN 300

Query: 301 RTFETYVFSLFNENLKPSVSERNYGLFKPDLTPVYDVGILRNSPVNPFFIYLNPLASAGI 360
           RTFETYVFSLFNENLKPSVSERNYGLFKPDLTPVYDVGILRNS                 
Sbjct: 301 RTFETYVFSLFNENLKPSVSERNYGLFKPDLTPVYDVGILRNS----------------- 360

Query: 361 MPNPTTGPTAPAGQAPQPSDGGRKWCVPKTDASDEALQKNIDYVCSSGVDCGPIQAGGAC 420
              PTTGPTAP GQAPQPSDGGRKWCVPKTDASDEALQKNIDYVCSSGVDCGPIQAGGAC
Sbjct: 361 ---PTTGPTAPTGQAPQPSDGGRKWCVPKTDASDEALQKNIDYVCSSGVDCGPIQAGGAC 420

Query: 421 YDPNTVRSHASYAMDAYFQSSGRHDFNCDFNHTGVLTSTDPSYKACSYPLGAEKLTEKSV 480
           YDPNTVRSHASYAMDAYFQSSGRHDFNCDFNHTGVLTSTDPSYKACSYPLGAEKLTEKSV
Sbjct: 421 YDPNTVRSHASYAMDAYFQSSGRHDFNCDFNHTGVLTSTDPSYKACSYPLGAEKLTEKSV 480

Query: 481 ASAAAMRRRIRLLGAVARTCIAVVAGGVLLI 512
           ASAAAMRRRIRLLGAVAR CIAVVAGGVLLI
Sbjct: 481 ASAAAMRRRIRLLGAVARACIAVVAGGVLLI 491

BLAST of Carg12372-RA vs. NCBI nr
Match: XP_011658156.1 (PREDICTED: glucan endo-1,3-beta-glucosidase [Cucumis sativus] >KGN65723.1 Glucan endo-1,3-beta-glucosidase [Cucumis sativus])

HSP 1 Score: 825.5 bits (2131), Expect = 1.0e-235
Identity = 415/511 (81.21%), Postives = 451/511 (88.26%), Query Frame = 0

Query: 1   MANSLLLSLLLFSLFLRLSFATYSIGVNYGTVADNLPPPSQVAAFLKSHTTIDRVKIFDA 60
           MA SL  SLLLF  F+ LS A YSIGVNYGTVA+NLPPPSQVAAFLKSHT+IDRVKIFDA
Sbjct: 1   MAYSLFFSLLLF-FFIPLSSAAYSIGVNYGTVANNLPPPSQVAAFLKSHTSIDRVKIFDA 60

Query: 61  NPDILRAFAATGIALTVSVANGDIPALAKLPAAQSWIANNILPFHPNTLINHIAVGNEIL 120
           NPDILRAFA TGIALTVSVANGDIPALAKLPAAQSW+ANNILPFHP TLINHIAVGNEIL
Sbjct: 61  NPDILRAFAGTGIALTVSVANGDIPALAKLPAAQSWVANNILPFHPTTLINHIAVGNEIL 120

Query: 121 ATSDKNLIAHMLPAMKALHSALTLANITDIKVSSPHSLGILSASEPPSTGRFRKGYDRAI 180
           ATSDKNLIAH+LPAMKA+HSAL LA+I+D+KVS+PHSLGILSASEPPSTGRFR+GYDRAI
Sbjct: 121 ATSDKNLIAHLLPAMKAIHSALKLAHISDVKVSTPHSLGILSASEPPSTGRFRRGYDRAI 180

Query: 181 FAPILEFHRKTKTPFMVNPYPFFGFRPATLDYALFKPNAGFFDNTTGKHYTNMFDAQMDA 240
           FAPIL+FH +TKTPFMVNPYP+FGFRPATLDYALFKPN G FDN TGKHYTNMFDAQ+DA
Sbjct: 181 FAPILDFHNQTKTPFMVNPYPYFGFRPATLDYALFKPNPGVFDNATGKHYTNMFDAQLDA 240

Query: 241 VHAAMNKLGYGDVDIVVAETGWPSAGDPNQPGVSLENAISYNQNLVKHVNSGKGTPMMPN 300
           V++AM K+GYGDVDIVVAETGWPSAGDPNQ GVS+ENAISYN+NLVKHVNSGKGTP+MPN
Sbjct: 241 VYSAMKKVGYGDVDIVVAETGWPSAGDPNQAGVSMENAISYNRNLVKHVNSGKGTPLMPN 300

Query: 301 RTFETYVFSLFNENLKPSVSERNYGLFKPDLTPVYDVGILRNSPVNPFFIYLNPLASAGI 360
           RTFETY+FSLFNENLKPS SERNYGLF+PD TPVYDVGIL+N                  
Sbjct: 301 RTFETYIFSLFNENLKPSTSERNYGLFQPDFTPVYDVGILQNP----------------- 360

Query: 361 MPNPTTGPTAPAGQAPQPSDGGRKWCVPKTDASDEALQKNIDYVCSSGVDCGPIQAGGAC 420
              P  GPT+P G  P+PSD  R+WCVPKTDASDEALQKNIDYVCSSGVDCGPIQ GGAC
Sbjct: 361 ---PAMGPTSPVG-GPKPSDEEREWCVPKTDASDEALQKNIDYVCSSGVDCGPIQEGGAC 420

Query: 421 YDPNTVRSHASYAMDAYFQSSGRHDFNCDFNHTGVLTSTDPSYKACSYPLGAEKLTEKSV 480
           YDPNTVRSHASYAM+AYFQ++GRH+FNCDFNHT +LTSTDPSY+ACSYP   EKLTEKSV
Sbjct: 421 YDPNTVRSHASYAMNAYFQTAGRHEFNCDFNHTAILTSTDPSYEACSYPFDEEKLTEKSV 480

Query: 481 ASAAAMRRRIRLLGAVARTCIAVVAGGVLLI 512
           AS A +R R+    A+AR CIAVVAGGVLLI
Sbjct: 481 ASGATIRCRMVGAVALARACIAVVAGGVLLI 489

BLAST of Carg12372-RA vs. NCBI nr
Match: XP_008457482.2 (PREDICTED: glucan endo-1,3-beta-glucosidase [Cucumis melo])

HSP 1 Score: 820.5 bits (2118), Expect = 3.3e-234
Identity = 415/516 (80.43%), Postives = 450/516 (87.21%), Query Frame = 0

Query: 1   MANSLLLSLL-LFSLFLRLSFATYSIGVNYGTVADNLPPPSQVAAFLKSHTTIDRVKIFD 60
           MA SL LSLL      + LS A YSIGVNYGTVA+NLPPP+QVAAFLKS T+IDRVKIFD
Sbjct: 1   MAYSLFLSLLXXXXXXIPLSSAAYSIGVNYGTVANNLPPPTQVAAFLKSRTSIDRVKIFD 60

Query: 61  ANPDILRAFAATGIALTVSVANGDIPALAKLPAAQSWIANNILPFHPNTLINHIAVGNEI 120
           ANPDILRAFA TGIALTVS+ANGDIPALAKLPAAQSW+ANNILPFHP TLINHIAVGNEI
Sbjct: 61  ANPDILRAFAGTGIALTVSIANGDIPALAKLPAAQSWVANNILPFHPTTLINHIAVGNEI 120

Query: 121 LATSDKNLIAHMLPAMKALHSALTLANITDIKVSSPHSLGILSASEPPSTGRFRKGYDRA 180
           LATSDKNLIAH+LPAMKA+HSAL LANI+D+KVS+PHSLGILSASEPPSTGRFR+GYDRA
Sbjct: 121 LATSDKNLIAHLLPAMKAIHSALKLANISDVKVSTPHSLGILSASEPPSTGRFRRGYDRA 180

Query: 181 IFAPILEFHRKTKTPFMVNPYPFFGFRPATLDYALFKPNAGFFDNTTGKHYTNMFDAQMD 240
           IFAPIL+FH +TKTPFMVNPYPFFGFRPATLDYALFKPNAG FDN TGKHYTNMFDAQ+D
Sbjct: 181 IFAPILDFHNQTKTPFMVNPYPFFGFRPATLDYALFKPNAGVFDNATGKHYTNMFDAQLD 240

Query: 241 AVHAAMNKLGYGDVDIVVAETGWPSAGDPNQPGVSLENAISYNQNLVKHVNSGKGTPMMP 300
           AVH+AM K+G+GDVDIVVAETGWPSAGDPNQPGVS+ENAISYN+NLVKHVNSGKGTP+MP
Sbjct: 241 AVHSAMKKVGFGDVDIVVAETGWPSAGDPNQPGVSMENAISYNRNLVKHVNSGKGTPLMP 300

Query: 301 NRTFETYVFSLFNENLKPSVSERNYGLFKPDLTPVYDVGILRNSPVNPFFIYLNPLASAG 360
           NRTFETY+FSLFNENLKP+ SERNYGLF+PD TPVYDVGILRN                 
Sbjct: 301 NRTFETYIFSLFNENLKPTTSERNYGLFQPDFTPVYDVGILRNP---------------- 360

Query: 361 IMPNPTTGPTAPAGQAPQPSDGGRKWCVPKTDASDEALQKNIDYVCSSGVDCGPIQAGGA 420
               PT GPT+P G  P+PSD GR+WCVPKTDASDEALQKNIDYVCSSGVDCGPIQ GGA
Sbjct: 361 ----PTMGPTSPVG-GPKPSDEGRRWCVPKTDASDEALQKNIDYVCSSGVDCGPIQEGGA 420

Query: 421 CYDPNTVRSHASYAMDAYFQSSGRHDFNCDFNHTGVLTSTDPSYKACSYPLGAEKLTEKS 480
           CYDPNTVRSHASYAM+AYFQ++GRHDFNCDFNHT +LT TDPSY+ACSYP   EKL EKS
Sbjct: 421 CYDPNTVRSHASYAMNAYFQTAGRHDFNCDFNHTAILTFTDPSYEACSYPFDEEKLAEKS 480

Query: 481 VASAAAMR----RRIRLLGAVARTCIAVVAGGVLLI 512
           VASAA +R      + +  AVAR  IAVVAGGVLLI
Sbjct: 481 VASAATIRCSMVGAVAVAVAVARAFIAVVAGGVLLI 495

BLAST of Carg12372-RA vs. TAIR10
Match: AT5G24318.1 (O-Glycosyl hydrolases family 17 protein)

HSP 1 Score: 478.0 bits (1229), Expect = 7.2e-135
Identity = 238/467 (50.96%), Postives = 329/467 (70.45%), Query Frame = 0

Query: 4   SLLLSLLLFSLFLRLSFATYSIGVNYGTVADNLPPPSQVAAFLKSHTTIDRVKIFDANPD 63
           SL +  ++  L +  + +  SIGVNYGT+A+NLPPP QVA FL   T I+R+++FD +P 
Sbjct: 9   SLSMFTIVGVLLILSTGSEASIGVNYGTLANNLPPPQQVAEFLLHSTVINRIRLFDTDPQ 68

Query: 64  ILRAFAATGIALTVSVANGDIPALAKLPAAQSWIANNILPFHPNTLINHIAVGNEILATS 123
           IL+AFA TGIA+TV+V N  IP L  L +A+ WI+++I P  P+T I  I VGNE+++T+
Sbjct: 69  ILQAFANTGIAVTVTVPNDQIPHLTNLSSAKQWISDHIQPHFPSTNIIRILVGNEVISTA 128

Query: 124 DKNLIAHMLPAMKALHSALTLANI-TDIKVSSPHSLGILSASEPPSTGRFRKGYDRAIFA 183
           D  LI  ++PAM++LH+AL  A++   I++S+PHSLG L+ S PPS+ +FR+GYD  +  
Sbjct: 129 DHLLIRTLIPAMQSLHTALVSASLHRRIQISTPHSLGTLTNSTPPSSAKFRRGYDAQVLK 188

Query: 184 PILEFHRKTKTPFMVNPYPFFGFRPATLDYALFKPNAGFFDNTTGKHYTNMFDAQMDAVH 243
           P+L F R T +PF+VNPYPFFG+   TLD+ALF+PN G FD  T   YTNM DAQ+D+V+
Sbjct: 189 PLLSFLRSTSSPFVVNPYPFFGYSIETLDFALFRPNPGLFDQHTKLLYTNMLDAQLDSVY 248

Query: 244 AAMNKLGYGDVDIVVAETGWPSAGDPNQPGVSLENAISYNQNLVKHVNSGKGTPMMPNRT 303
           +AM+KLG+ DV+IV+ E GWPS GD +Q GV ++ A  +N+NL+  V+SG GTP+MPNRT
Sbjct: 249 SAMDKLGFSDVEIVIGEIGWPSEGDIDQIGVDVDTAAEFNKNLIARVDSGTGTPLMPNRT 308

Query: 304 FETYVFSLFNENLKPS-VSERNYGLFKPDLTPVYDVGILRNSPVNPFFIYLNPLASAGIM 363
           FETY+F+LFNENLK    SERN+G+F+ DLTP+YD+GILR     P F   +P+      
Sbjct: 309 FETYIFALFNENLKSGPTSERNFGIFRSDLTPIYDIGILR-----PTFRSSDPVY----- 368

Query: 364 PNPTTGPTAPAGQAPQPSDGGRKWCVPKTDASDEALQKNIDYVCSSGVDCGPIQAGGACY 423
            NP         ++P      ++WCV K  A   ALQ+NIDYVC  G+DC PI  GG CY
Sbjct: 369 -NP---------RSPVRGSSSKRWCVTKAGAETVALQRNIDYVCGLGLDCRPINEGGLCY 428

Query: 424 DPNTVRSHASYAMDAYFQSSGRHDFNCDFNHTGVLTSTDPSYKACSY 469
            PNTV++H+ YAM+ Y+Q+  +H+F+CDF++TG +T+ DPSY  C Y
Sbjct: 429 LPNTVKAHSKYAMNLYYQTMEKHEFDCDFDNTGEITTIDPSYGNCEY 455

BLAST of Carg12372-RA vs. TAIR10
Match: AT3G55430.1 (O-Glycosyl hydrolases family 17 protein)

HSP 1 Score: 465.3 bits (1196), Expect = 4.8e-131
Identity = 245/467 (52.46%), Postives = 323/467 (69.16%), Query Frame = 0

Query: 6   LLSLLLFSLFLRLSFATYSIGVNYGTVADNLPPPSQVAAFLKSHTTIDRVKIFDANPDIL 65
           LL LL  ++FL +     +IGVNYGT+  NLPPP+QVA F+K+ T+ID VKIFD NPDIL
Sbjct: 10  LLLLLCAAVFLTIPAVISAIGVNYGTLG-NLPPPTQVANFIKTQTSIDSVKIFDVNPDIL 69

Query: 66  RAFAATGIALTVSVANGDIPALAKLPAAQSWIANNILPFHPNTLINHIAVGNEILATSDK 125
           RAFA TGI++ V+V NGDIPALA    A+ W++ NILPFHP T I +I+VGNEIL T D 
Sbjct: 70  RAFAGTGISVVVTVPNGDIPALANGRQARRWVSVNILPFHPQTKIKYISVGNEILLTGDN 129

Query: 126 NLIAHMLPAMKALHSALTLANITDIKVSSPHSLGILS--ASEPPSTGRFRKGYDRAIFAP 185
           N+I ++LPAM+ L++AL  A + D+KV++ HSL I++   +  PS+GRFR G+D+ I AP
Sbjct: 130 NMINNLLPAMRNLNNALVRAGVRDVKVTTAHSLNIIAYDLTGAPSSGRFRPGWDKGILAP 189

Query: 186 ILEFHRKTKTPFMVNPYPFFGFRPATLDYALFK-PNAGFFDNTTGKHYTNMFDAQMDAVH 245
           IL +HR+TK+PFMVNPYP+FGF P  +++A+F+ P     D  T   YTNMFDA MD+ +
Sbjct: 190 ILAYHRRTKSPFMVNPYPYFGFDPKNVNFAIFRTPYKAVRDPFTRHVYTNMFDALMDSTY 249

Query: 246 AAMNKLGYGDVDIVVAETGWPSAGDPNQPGVSLENAISYNQNLVKHVNSGKGTPMMPNRT 305
           +AM  LGYGDV+IVV ETGWPSA D   P  S  NA  +N N++K    G+GTP+MPNR 
Sbjct: 250 SAMKALGYGDVNIVVGETGWPSACD--APWCSPANAAWFNLNIIKRA-QGQGTPLMPNRR 309

Query: 306 FETYVFSLFNENLKPS-VSERNYGLFKPDLTPVYDVGILRNSPVNPFFIYLNPLASAGIM 365
           FETY+F LFNE  KP   +ERN+GLF+ D +PVYDVG+LRN                   
Sbjct: 310 FETYIFGLFNEEGKPGPTAERNWGLFRADFSPVYDVGLLRNG------------------ 369

Query: 366 PNPTTGPTAPAGQAPQPSDGGRKWCVPKTDASDEALQKNIDYVCSSGVDCGPIQAGGACY 425
                G   PA   P PS  G KWCV ++ A++  LQ +I++VC  GVDC PIQAGG+C+
Sbjct: 370 ---QGGGGRPA--LPAPSTAGGKWCVARSGATNTQLQDSINWVCGQGVDCKPIQAGGSCF 429

Query: 426 DPNTVRSHASYAMDAYFQSSGRHDFNCDFNHTGVLTSTDPSYKACSY 469
           +P+++R+HAS+ M+AYFQS GR D  C+F+ TG++   +PS  AC Y
Sbjct: 430 NPSSLRTHASFVMNAYFQSHGRTDGACNFSGTGMIVGNNPSNGACKY 449

BLAST of Carg12372-RA vs. TAIR10
Match: AT3G46570.1 (Glycosyl hydrolase superfamily protein)

HSP 1 Score: 450.7 bits (1158), Expect = 1.2e-126
Identity = 227/342 (66.37%), Postives = 278/342 (81.29%), Query Frame = 0

Query: 7   LSLLLFSLFLRLSFATYSIGVNYGTVADNLPPPSQVAAFLKSHTTIDRVKIFDANPDILR 66
           L  L+ +L L  S A Y +GVNYGTVA+NLPPP QV  F+K+ T I+ VKIFD N DIL 
Sbjct: 10  LYFLILTLILPFS-AAYQVGVNYGTVANNLPPPHQVVNFIKTKTIINHVKIFDTNHDILA 69

Query: 67  AFAA-TGIALTVSVANGDIPALAKLPAAQSWIANNILPFHPNTLINHIAVGNEILATSDK 126
           AF+  TGI+LTV+V N DI +L+KL  A+SW+++N+LPF   T I +IAVGNE++ATSDK
Sbjct: 70  AFSGITGISLTVTVPNSDIISLSKLSNARSWLSDNLLPFLLTTSIRYIAVGNEVVATSDK 129

Query: 127 NLIAHMLPAMKALHSALTLANITDIKVSSPHSLGILS-ASEPPSTGRFRKGYDRAIFAPI 186
            LI H+LPAM+ L  AL LAN++ I VS+PHSLGILS +SEPPS+G+FRKGYD+AIF+PI
Sbjct: 130 TLITHLLPAMETLTLALHLANVSRILVSTPHSLGILSGSSEPPSSGKFRKGYDKAIFSPI 189

Query: 187 LEFHRKTKTPFMVNPYPFFGFRPATLDYALFKPN-AGFFDNTTGKHYTNMFDAQMDAVHA 246
           L+FH +TK+PFMVNPYP+FGF P TL+YALF  N   + D  T  +YTNMFDAQ+DAV++
Sbjct: 190 LDFHNRTKSPFMVNPYPYFGFGPETLNYALFNTNDVVYVDPVTKLNYTNMFDAQLDAVYS 249

Query: 247 AMNKLGYGDVDIVVAETGWPSAGDPNQPGVSLENAISYNQNLVKHVNSGKGTPMMPNRTF 306
           AM + GYGDVDIVVAETGWPSAG+PNQ GV L+ A +YN NL+KHVNSGKGTP+MPNR F
Sbjct: 250 AMKRFGYGDVDIVVAETGWPSAGEPNQTGVGLDYAAAYNGNLIKHVNSGKGTPLMPNRVF 309

Query: 307 ETYVFSLFNENLKPSVSERNYGLFKPDLTPVYDVGILRNSPV 346
           ETYVFSLFNENLK SVSE+N+GLFKPD TPVYDVGI++ + V
Sbjct: 310 ETYVFSLFNENLKSSVSEQNFGLFKPDFTPVYDVGIMKTNKV 350

BLAST of Carg12372-RA vs. TAIR10
Match: AT4G34480.1 (O-Glycosyl hydrolases family 17 protein)

HSP 1 Score: 416.8 bits (1070), Expect = 2.0e-116
Identity = 219/468 (46.79%), Postives = 302/468 (64.53%), Query Frame = 0

Query: 9   LLLFSLFLRLSFATYSIGVNYGTVADNLPPPSQVAAFLKSHTTIDRVKIFDANPDILRAF 68
           LL+F      S A   IGVNYG VADNLPPPS+    L+S T+I +V+++ A+P I++A 
Sbjct: 10  LLIFLSHFPSSHAEPFIGVNYGQVADNLPPPSETVKLLQS-TSIQKVRLYGADPAIIKAL 69

Query: 69  AATGIALTVSVANGDIPALAKLP-AAQSWIANNILPFHPNTLINHIAVGNEILATSDKNL 128
           A TG+ + +  ANGD+P+LA  P AA  WI +N+LPF+P + I  I VGNEIL ++D NL
Sbjct: 70  AGTGVGIVIGAANGDVPSLASDPNAATQWINSNVLPFYPASKIMLITVGNEILMSNDPNL 129

Query: 129 IAHMLPAMKALHSALTLANI-TDIKVSSPHSLGILSASEPPSTGRFRKGYDRAIFAPILE 188
           +  +LPAM+ +  AL   ++   IKVS+ +S+ +L +S+PPS+G F  GY   +   IL+
Sbjct: 130 VNQLLPAMQNVQKALEAVSLGGKIKVSTVNSMTVLGSSDPPSSGSFAAGYQTGL-KGILQ 189

Query: 189 FHRKTKTPFMVNPYPFFGF----RPATLDYALFKPNAGFFDNTTGKHYTNMFDAQMDAVH 248
           F   T +PF +NPYPFF +    RP TL + LF+PNAG  D+ TG  YTNMFDAQ+DAVH
Sbjct: 190 FLSDTGSPFAINPYPFFAYQSDPRPETLAFCLFEPNAGRVDSKTGIKYTNMFDAQVDAVH 249

Query: 249 AAMNKLGYGDVDIVVAETGWPSAGDPNQPGVSLENAISYNQNLVKHVNSGKGTPMMPNRT 308
           +A+  +G+  V+IVVAETGW S GD N+ G S++NA +YN NL+ H+ S  GTP+MP + 
Sbjct: 250 SALKSMGFEKVEIVVAETGWASRGDANEVGASVDNAKAYNGNLIAHLRSMVGTPLMPGKP 309

Query: 309 FETYVFSLFNENLKPS-VSERNYGLFKPDLTPVYDVGILRNSPVNPFFIYLNPLASAGIM 368
            +TY+F+L++ENLKP   SER +GLFK DL+ VYDVG+ ++S                  
Sbjct: 310 VDTYIFALYDENLKPGPSSERAFGLFKTDLSMVYDVGLAKSS------------------ 369

Query: 369 PNPTTGPTAPAGQAPQPSDGGRKWCVPKTDASDEALQKNIDYVCSSGVDCGPIQAGGACY 428
                     + Q P        WCVPK  A++E LQ ++D+ C  G+DCG IQ GGAC+
Sbjct: 370 ---------SSSQTPSGKVTSSGWCVPKKGATNEELQASLDWACGHGIDCGAIQPGGACF 429

Query: 429 DPNTVRSHASYAMDAYFQSSGRHDFNCDFNHTGVLTSTDPSYKACSYP 470
           +PN V SHA+YAM+ YFQ S +   +CDF+ T  +TS +PSY  C YP
Sbjct: 430 EPNNVVSHAAYAMNMYFQKSPKQPTDCDFSKTATVTSQNPSYNNCVYP 448

BLAST of Carg12372-RA vs. TAIR10
Match: AT2G16230.1 (O-Glycosyl hydrolases family 17 protein)

HSP 1 Score: 413.7 bits (1062), Expect = 1.7e-115
Identity = 217/476 (45.59%), Postives = 306/476 (64.29%), Query Frame = 0

Query: 1   MANSLLLSLLLFSLFLRLSFATYSIGVNYGTVADNLPPPSQVAAFLKSHTTIDRVKIFDA 60
           MA S+L  LL     +  S A   IGVNYG ++DNLPPPSQ A  L+S T+I +V++++A
Sbjct: 1   MALSILFLLLFILFSISPSNAQSFIGVNYGLLSDNLPPPSQTAKLLQS-TSIQKVRLYNA 60

Query: 61  NPDILRAFAATGIALTVSVANGDIPALAK-LPAAQSWIANNILPFHPNTLINHIAVGNEI 120
           +  I+ +   TGI + + VANGD+P++A  L  A  WI +N+LPF+P + I  I VGNE+
Sbjct: 61  DSSIITSLVGTGIGIVIGVANGDLPSIASDLNIASQWINSNVLPFYPASNIILINVGNEV 120

Query: 121 LATSDKNLIAHMLPAMKALHSALTLANI-TDIKVSSPHSLGILSASEPPSTGRFRKGYDR 180
           L ++D NL+  +LPAM+ +  AL   ++   IKVS+ H++ +L  SEPPS G F   Y +
Sbjct: 121 LLSNDLNLVNQLLPAMQNVQKALEAVSLGGKIKVSTVHAMTVLGNSEPPSAGSFAPSY-Q 180

Query: 181 AIFAPILEFHRKTKTPFMVNPYPFFGF----RPATLDYALFKPNAGFFDNTTGKHYTNMF 240
           A    IL+F   T +PF +NPYPFF +    RP TL + LF+PN G  D+ TG  Y NMF
Sbjct: 181 AGLKGILQFLSDTGSPFAINPYPFFAYQSDPRPETLAFCLFQPNPGRVDSNTGIKYMNMF 240

Query: 241 DAQMDAVHAAMNKLGYGDVDIVVAETGWPSAGDPNQPGVSLENAISYNQNLVKHVNSGKG 300
           DAQ+DAVH+A+  +G+  V+++VAETGWPS GD N+ G S+ENA +YN NL+ H+ S  G
Sbjct: 241 DAQVDAVHSALKSIGFEKVEVLVAETGWPSTGDSNEVGPSVENAKAYNGNLIAHLRSMVG 300

Query: 301 TPMMPNRTFETYVFSLFNENLKPSVS-ERNYGLFKPDLTPVYDVGILRNSPVNPFFIYLN 360
           TP+MP ++ +TY+F+LF+ENLKP  S E+++GLFKPDL+  YD+G+ + +          
Sbjct: 301 TPLMPGKSIDTYIFALFDENLKPGPSFEQSFGLFKPDLSMAYDIGLTKTT---------- 360

Query: 361 PLASAGIMPNPTTGPTAPAGQAPQPSDGGRKWCVPKTDASDEALQKNIDYVCSSGVDCGP 420
              S+    +P  G     G           WCVPK DA+ E LQ ++D+VC  G+DCGP
Sbjct: 361 ---SSQTSQSPQLGKVTSMG-----------WCVPKEDATQEQLQDSLDWVCGQGIDCGP 420

Query: 421 IQAGGACYDPNTVRSHASYAMDAYFQSSGRHDFNCDFNHTGVLTSTDPSYKACSYP 470
           I  GG C++PN V SH +YAM+ YFQ S  +  +CDF+ T  +TS +PSY +C YP
Sbjct: 421 IMPGGVCFEPNNVASHTAYAMNLYFQKSPENPTDCDFSKTARITSENPSYSSCVYP 450

BLAST of Carg12372-RA vs. Swiss-Prot
Match: sp|P52409|E13B_WHEAT (Glucan endo-1,3-beta-glucosidase OS=Triticum aestivum OX=4565 GN=GLC1 PE=2 SV=1)

HSP 1 Score: 458.8 bits (1179), Expect = 8.1e-128
Identity = 230/450 (51.11%), Postives = 304/450 (67.56%), Query Frame = 0

Query: 25  IGVNYGTVADNLPPPSQVAAFLKSHTTIDRVKIFDANPDILRAFAATGIALTVSVANGDI 84
           IGVNYG  ADNLP P+ VA FL + TTIDRVK+FDANP  + AFA T I+L VS+ N  +
Sbjct: 26  IGVNYGANADNLPSPTSVATFLATKTTIDRVKLFDANPTFISAFAGTPISLAVSLPNSAL 85

Query: 85  PALAK----LPAAQSWIANNILPFHPNTLINHIAVGNEILATSDKNLIAHMLPAMKALHS 144
           PALA     L AA+SWI  N+ P+ P T +  +  GNEIL ++D NLI  +LPAM+ L  
Sbjct: 86  PALADKATGLDAARSWIRANLSPYVPATNVTLLLAGNEILLSTDTNLILSLLPAMRRLAQ 145

Query: 145 ALTLANITDIKVSSPHSLGILSASEP-PSTGRFRKGYDRAIFAPILEFHRKTKTPFMVNP 204
           AL    +T ++V++PH LGIL+ S+  PS   FR GY+  +F  +L+FHR T +PFMVNP
Sbjct: 146 ALKAEGLTGVRVTTPHYLGILAPSDGIPSNASFRAGYNTKLFPAMLQFHRDTGSPFMVNP 205

Query: 205 YPFFGFRPATLDYALFKPNAGFFDNTTGKHYTNMFDAQMDAVHAAMNKLGYGDVDIVVAE 264
           YP+F +RP TL+YALF+PN+G +D  T  +YT+M DAQMDA++ AM KLGYGDVDI V E
Sbjct: 206 YPYFSYRPETLNYALFRPNSGIYDPATKLNYTSMLDAQMDAIYTAMKKLGYGDVDIAVGE 265

Query: 265 TGWPSAGDPNQPGVSLENAISYNQNLVKHVNSGKGTPMMPNRTFETYVFSLFNENLKPS- 324
            GWP+  +P Q GV ++ A  +N+ +++  +SGKGTP+MPNRTFETY+FSLF+EN KP  
Sbjct: 266 AGWPTQAEPGQIGVGVQEARDFNEGMIRVCSSGKGTPLMPNRTFETYLFSLFDENQKPGP 325

Query: 325 VSERNYGLFKPDLTPVYDVGILRNSPVNPFFIYLNPLASAGIMPNPTTGPTAPAGQAPQP 384
           ++ER++GLF PD TPVYD+G+LR+               A + P                
Sbjct: 326 IAERHFGLFNPDFTPVYDLGLLRD--------------GASVAPXXXXXXXXXXXXXXXX 385

Query: 385 SDGGRKWCVPKTDASDEALQKNIDYVCSSGVDCGPIQAGGACYDPNTVRSHASYAMDAYF 444
             GG KWCV K  A+   LQ NI+Y C   VDC PIQ+GGAC+ PN++++HASY M+AY+
Sbjct: 386 XXGG-KWCVAKDGANGTDLQNNINYACGF-VDCKPIQSGGACFSPNSLQAHASYVMNAYY 445

Query: 445 QSSGRHDFNCDFNHTGVLTSTDPSYKACSY 469
           Q++G  D  CDF  TG++TS+DPSY  C Y
Sbjct: 446 QANGHTDLACDFKGTGIVTSSDPSYGGCKY 459

BLAST of Carg12372-RA vs. Swiss-Prot
Match: sp|Q94G86|ALL9_OLEEU (Glucan endo-1,3-beta-D-glucosidase OS=Olea europaea OX=4146 GN=OLE9 PE=1 SV=1)

HSP 1 Score: 419.5 bits (1077), Expect = 5.5e-116
Identity = 220/484 (45.45%), Postives = 315/484 (65.08%), Query Frame = 0

Query: 1   MANSLLLSLLLFSLFLRLS--FATYS---IGVNYGTVADNLPPPSQVAAFLKSHTTIDRV 60
           MA ++  S LLF +FL L   ++  S   +GVNYG ++DNLP        LKS TTI +V
Sbjct: 1   MAANVQTSSLLFLVFLLLQNFYSANSQSFLGVNYGQLSDNLPSLQATVNLLKS-TTIQKV 60

Query: 61  KIFDANPDILRAFAATGIALTVSVANGDIPALAKLP-AAQSWIANNILPFHPNTLINHIA 120
           ++F A P +++AFA TG+ + +   NGDIP LA  P  A  ++ +N++ F+P + I  I 
Sbjct: 61  RLFGAEPAVIKAFANTGVEIVIGFDNGDIPTLASNPNVASQFVKSNVMSFYPASNIIAIT 120

Query: 121 VGNEILATSDKNLIAHMLPAMKALHSALTLANI-TDIKVSSPHSLGILSASEPPSTGRFR 180
           VGNE+L + D+ LI+ +LPAM+ + +AL  A++   +KVS+ H++ +LS S PPS+G F 
Sbjct: 121 VGNEVLTSGDQKLISQLLPAMQNVQNALNAASLGGKVKVSTVHAMAVLSQSYPPSSGVFN 180

Query: 181 KGYDRAIFAPILEFHRKTKTPFMVNPYPFFGFR----PATLDYALFKPNAGFFDNTTGKH 240
            G    + A +L+F      PFM++PYP+F ++    P TL + LF+PNAG  D+  G  
Sbjct: 181 PGLGDTMKA-LLQFQSANDAPFMISPYPYFAYKNQPTPDTLAFCLFQPNAGQVDSGNGHK 240

Query: 241 YTNMFDAQMDAVHAAMNKLGYGDVDIVVAETGWPSAGDPNQPGVSLENAISYNQNLVKHV 300
           YTNMFDAQ+DAVH+A+N +G+ D++IVVAETGWP  GD N+ G SL+NA +Y  NL+ H+
Sbjct: 241 YTNMFDAQVDAVHSALNAMGFKDIEIVVAETGWPHGGDSNEVGPSLDNAKAYVGNLINHL 300

Query: 301 NSGKGTPMMPNRTFETYVFSLFNENLKPSV-SERNYGLFKPDLTPVYDVGILRNSPVNPF 360
            S  GTP+MP ++ +TY+FSL++E+ K    SE+ +GLFKPD +  YDVG+L+N      
Sbjct: 301 KSKVGTPLMPGKSIDTYLFSLYDEDKKTGASSEKYFGLFKPDGSTTYDVGLLKN------ 360

Query: 361 FIYLNPLASAGIMPNPTTGPTAPAGQAPQPSDGGRKWCVPKTDASDEALQKNIDYVCSSG 420
                           T  PT PA   P P   G  WCVPK   SD+ L  NI+Y C  G
Sbjct: 361 ----------------TQNPTTPATPTPTPKAAG-SWCVPKPGVSDDQLTGNINYACGQG 420

Query: 421 VDCGPIQAGGACYDPNTVRSHASYAMDAYFQSSGRHDFNCDFNHTGVLTSTDPSYKACSY 473
           +DCGPIQ GGAC++PNTV++HA+Y M+ Y+QS+GR+ +NCDF+ T  LT+T+PSY AC++
Sbjct: 421 IDCGPIQPGGACFEPNTVKAHAAYVMNLYYQSAGRNSWNCDFSQTATLTNTNPSYGACNF 459

BLAST of Carg12372-RA vs. Swiss-Prot
Match: sp|Q9M069|E137_ARATH (Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 PE=1 SV=2)

HSP 1 Score: 416.8 bits (1070), Expect = 3.5e-115
Identity = 219/468 (46.79%), Postives = 302/468 (64.53%), Query Frame = 0

Query: 9   LLLFSLFLRLSFATYSIGVNYGTVADNLPPPSQVAAFLKSHTTIDRVKIFDANPDILRAF 68
           LL+F      S A   IGVNYG VADNLPPPS+    L+S T+I +V+++ A+P I++A 
Sbjct: 10  LLIFLSHFPSSHAEPFIGVNYGQVADNLPPPSETVKLLQS-TSIQKVRLYGADPAIIKAL 69

Query: 69  AATGIALTVSVANGDIPALAKLP-AAQSWIANNILPFHPNTLINHIAVGNEILATSDKNL 128
           A TG+ + +  ANGD+P+LA  P AA  WI +N+LPF+P + I  I VGNEIL ++D NL
Sbjct: 70  AGTGVGIVIGAANGDVPSLASDPNAATQWINSNVLPFYPASKIMLITVGNEILMSNDPNL 129

Query: 129 IAHMLPAMKALHSALTLANI-TDIKVSSPHSLGILSASEPPSTGRFRKGYDRAIFAPILE 188
           +  +LPAM+ +  AL   ++   IKVS+ +S+ +L +S+PPS+G F  GY   +   IL+
Sbjct: 130 VNQLLPAMQNVQKALEAVSLGGKIKVSTVNSMTVLGSSDPPSSGSFAAGYQTGL-KGILQ 189

Query: 189 FHRKTKTPFMVNPYPFFGF----RPATLDYALFKPNAGFFDNTTGKHYTNMFDAQMDAVH 248
           F   T +PF +NPYPFF +    RP TL + LF+PNAG  D+ TG  YTNMFDAQ+DAVH
Sbjct: 190 FLSDTGSPFAINPYPFFAYQSDPRPETLAFCLFEPNAGRVDSKTGIKYTNMFDAQVDAVH 249

Query: 249 AAMNKLGYGDVDIVVAETGWPSAGDPNQPGVSLENAISYNQNLVKHVNSGKGTPMMPNRT 308
           +A+  +G+  V+IVVAETGW S GD N+ G S++NA +YN NL+ H+ S  GTP+MP + 
Sbjct: 250 SALKSMGFEKVEIVVAETGWASRGDANEVGASVDNAKAYNGNLIAHLRSMVGTPLMPGKP 309

Query: 309 FETYVFSLFNENLKPS-VSERNYGLFKPDLTPVYDVGILRNSPVNPFFIYLNPLASAGIM 368
            +TY+F+L++ENLKP   SER +GLFK DL+ VYDVG+ ++S                  
Sbjct: 310 VDTYIFALYDENLKPGPSSERAFGLFKTDLSMVYDVGLAKSS------------------ 369

Query: 369 PNPTTGPTAPAGQAPQPSDGGRKWCVPKTDASDEALQKNIDYVCSSGVDCGPIQAGGACY 428
                     + Q P        WCVPK  A++E LQ ++D+ C  G+DCG IQ GGAC+
Sbjct: 370 ---------SSSQTPSGKVTSSGWCVPKKGATNEELQASLDWACGHGIDCGAIQPGGACF 429

Query: 429 DPNTVRSHASYAMDAYFQSSGRHDFNCDFNHTGVLTSTDPSYKACSYP 470
           +PN V SHA+YAM+ YFQ S +   +CDF+ T  +TS +PSY  C YP
Sbjct: 430 EPNNVVSHAAYAMNMYFQKSPKQPTDCDFSKTATVTSQNPSYNNCVYP 448

BLAST of Carg12372-RA vs. Swiss-Prot
Match: sp|Q8VYE5|E1312_ARATH (Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360 PE=2 SV=1)

HSP 1 Score: 320.1 bits (819), Expect = 4.5e-86
Identity = 186/474 (39.24%), Postives = 271/474 (57.17%), Query Frame = 0

Query: 6   LLSLLLFSLFLRLSFATYS-IGVNYGTVADNLPPPSQVAAFLKSHTTIDRVKIFDANPDI 65
           L+  +  S+   L+F   S IG+ YG  ADNLP P++V+  ++ H  I  V+I+DAN D+
Sbjct: 7   LVFWIFVSILAFLNFGMASKIGICYGRNADNLPSPNRVSELIQ-HLNIKFVRIYDANIDV 66

Query: 66  LRAFAATGIALTVSVANGDIPALAKLPA-AQSWIANNILPFHPNTLINHIAVGNEILATS 125
           L+AFA TGI L + V N D+ A A+  +   +W++NNILP++P+T I  I+VG E+    
Sbjct: 67  LKAFANTGIELMIGVPNADLLAFAQFQSNVDTWLSNNILPYYPSTKITSISVGLEVTEAP 126

Query: 126 DKNLIAHMLPAMKALHSALTLANI-TDIKVSSPHSLGILSASEPPSTGRFRKGYDRAIFA 185
           D N    +LPAM+ +H+AL  + +   IK+SS HSL ILS S PPS+  F K +  A   
Sbjct: 127 D-NATGLVLPAMRNIHTALKKSGLDKKIKISSSHSLAILSRSFPPSSASFSKKHS-AFLK 186

Query: 186 PILEFHRKTKTPFMVNPYPFFGFRPAT----LDYALFKPNAGFFDNTTGKHYTNMFDAQM 245
           P+LEF  + ++PFM++ YP++ +R +T    L+YALF+ ++   D  TG  Y+NMFDAQ+
Sbjct: 187 PMLEFLVENESPFMIDLYPYYAYRDSTEKVPLEYALFESSSQVVDPATGLLYSNMFDAQL 246

Query: 246 DAVHAAMNKLGYGDVDIVVAETGWPSAGDPNQPGVSLENAISYNQNLVKHVNSGKGTPMM 305
           DA++ A+  + +  V ++V E+GWPS G P +   + ENA++YN NL++HV    GTP  
Sbjct: 247 DAIYFALTAMSFKTVKVMVTESGWPSKGSPKETAATPENALAYNTNLIRHVIGDPGTPAK 306

Query: 306 PNRTFETYVFSLFNENLKPSV-SERNYGLFKPDLTPVYDVGIL--RNSPVNPFFIYLNPL 365
           P    + Y+FSLFNEN KP + SERN+G+F  + T VY +       +PV+P        
Sbjct: 307 PGEEIDVYLFSLFNENRKPGIESERNWGMFYANGTNVYALDFTGENTTPVSP--TNSTTX 366

Query: 366 ASAGIMPNPTTGPTAPAGQAPQPSDGGRKWCVPKTDASDEALQKNIDYVCSSG-VDCGPI 425
                                        WC+  + AS   LQ  +D+ C  G VDC  +
Sbjct: 367 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXWCIASSQASVTELQTALDWACGPGNVDCSAV 426

Query: 426 QAGGACYDPNTVRSHASYAMDAYFQSSGRHDFNCDFNHTGVLTSTDPSYKACSY 469
           Q    C++P+TV SHASYA + Y+Q SG    +C FN   V    DPSY  C Y
Sbjct: 427 QPDQPCFEPDTVLSHASYAFNTYYQQSGASSIDCSFNGASVEVDKDPSYGNCLY 475

BLAST of Carg12372-RA vs. Swiss-Prot
Match: sp|Q9ZU91|E133_ARATH (Glucan endo-1,3-beta-glucosidase 3 OS=Arabidopsis thaliana OX=3702 GN=At2g01630 PE=2 SV=2)

HSP 1 Score: 303.5 bits (776), Expect = 4.4e-81
Identity = 187/483 (38.72%), Postives = 278/483 (57.56%), Query Frame = 0

Query: 6   LLSLLLFSLFLRLSFATYS---IGVNYGTVADNLPPPSQVAAFLKSHTTIDRVKIFDANP 65
           + +LLL  LFL  S A      IGVN GT   N+P P+QV A LKS   I+RV+++DA+ 
Sbjct: 1   MAALLLLFLFLFASSALSQDSLIGVNIGTEVTNMPSPTQVVALLKSQ-NINRVRLYDADR 60

Query: 66  DILRAFAATGIALTVSVANGDIPALAKLPA-AQSWIANNILPFHPNTLINHIAVGNEILA 125
            +L AFA TG+ + +SV N  +  +++  A A +W+  N+  ++P T I  IAVG+E+L 
Sbjct: 61  SMLLAFAHTGVQVIISVPNDQLLGISQSNATAANWVTRNVAAYYPATNITTIAVGSEVL- 120

Query: 126 TSDKNLIAHMLPAMKALHSALTLANI-TDIKVSSPHSLGILSASEPPSTGRFRKGYDRAI 185
           TS  N  + ++ A+K + +AL  AN+   IKVS+PHS  I+  S PPS   F K +D  +
Sbjct: 121 TSLTNAASVLVSALKYIQAALVTANLDRQIKVSTPHSSTIILDSFPPSQAFFNKTWD-PV 180

Query: 186 FAPILEFHRKTKTPFMVNPYPFFGFRPAT----LDYALFKP---NAGFFDNTTGKHYTNM 245
             P+L+F + T +P ++N YP+F +  +     LDYALF+P   N    D  T  HYTN+
Sbjct: 181 IVPLLKFLQSTGSPLLLNVYPYFDYVQSNGVIPLDYALFQPLQANKEAVDANTLLHYTNV 240

Query: 246 FDAQMDAVHAAMNKLGYGDVDIVVAETGWPSAGDPNQPGVSLENAISYNQNLVKHVNSGK 305
           FDA +DA + AM+ L + ++ IVV E+GWPS G P++   ++ENA +YN NL++HV +  
Sbjct: 241 FDAIVDAAYFAMSYLNFTNIPIVVTESGWPSKGGPSEHDATVENANTYNSNLIQHVINKT 300

Query: 306 GTPMMPNRTFETYVFSLFNENLKPS-VSERNYGLFKPDLTPVYDVGILRNSPVNPFFIYL 365
           GTP  P     TY++ L+NE+ +P  VSE+N+GLF  + TPVY +               
Sbjct: 301 GTPKHPGTAVTTYIYELYNEDTRPGPVSEKNWGLFYTNGTPVYTL--------------- 360

Query: 366 NPLASAG-IMPNPTTGPTAPAGQAPQPSDGGRKWCVPKTDASDEALQKNIDYVCSSG-VD 425
             LA AG I+ N TT  T               +C+ K     + LQ  +D+ C  G VD
Sbjct: 361 -RLAGAGAILANDTTNQT---------------FCIAKEKVDRKMLQAALDWACGPGKVD 420

Query: 426 CGPIQAGGACYDPNTVRSHASYAMDAYFQSSGRHDFNCDFNHTGVLTSTDPSYKACSYPL 474
           C  +  G +CY+P+ V +H++YA +AY+Q  G+   +CDF     +T+TDPS   C +P 
Sbjct: 421 CSALMQGESCYEPDDVVAHSTYAFNAYYQKMGKASGSCDFKGVATVTTTDPSRGTCVFPG 449

BLAST of Carg12372-RA vs. TrEMBL
Match: tr|A0A0A0LVC1|A0A0A0LVC1_CUCSA (Glucan endo-1,3-beta-glucosidase OS=Cucumis sativus OX=3659 GN=Csa_1G522000 PE=3 SV=1)

HSP 1 Score: 825.5 bits (2131), Expect = 6.7e-236
Identity = 415/511 (81.21%), Postives = 451/511 (88.26%), Query Frame = 0

Query: 1   MANSLLLSLLLFSLFLRLSFATYSIGVNYGTVADNLPPPSQVAAFLKSHTTIDRVKIFDA 60
           MA SL  SLLLF  F+ LS A YSIGVNYGTVA+NLPPPSQVAAFLKSHT+IDRVKIFDA
Sbjct: 1   MAYSLFFSLLLF-FFIPLSSAAYSIGVNYGTVANNLPPPSQVAAFLKSHTSIDRVKIFDA 60

Query: 61  NPDILRAFAATGIALTVSVANGDIPALAKLPAAQSWIANNILPFHPNTLINHIAVGNEIL 120
           NPDILRAFA TGIALTVSVANGDIPALAKLPAAQSW+ANNILPFHP TLINHIAVGNEIL
Sbjct: 61  NPDILRAFAGTGIALTVSVANGDIPALAKLPAAQSWVANNILPFHPTTLINHIAVGNEIL 120

Query: 121 ATSDKNLIAHMLPAMKALHSALTLANITDIKVSSPHSLGILSASEPPSTGRFRKGYDRAI 180
           ATSDKNLIAH+LPAMKA+HSAL LA+I+D+KVS+PHSLGILSASEPPSTGRFR+GYDRAI
Sbjct: 121 ATSDKNLIAHLLPAMKAIHSALKLAHISDVKVSTPHSLGILSASEPPSTGRFRRGYDRAI 180

Query: 181 FAPILEFHRKTKTPFMVNPYPFFGFRPATLDYALFKPNAGFFDNTTGKHYTNMFDAQMDA 240
           FAPIL+FH +TKTPFMVNPYP+FGFRPATLDYALFKPN G FDN TGKHYTNMFDAQ+DA
Sbjct: 181 FAPILDFHNQTKTPFMVNPYPYFGFRPATLDYALFKPNPGVFDNATGKHYTNMFDAQLDA 240

Query: 241 VHAAMNKLGYGDVDIVVAETGWPSAGDPNQPGVSLENAISYNQNLVKHVNSGKGTPMMPN 300
           V++AM K+GYGDVDIVVAETGWPSAGDPNQ GVS+ENAISYN+NLVKHVNSGKGTP+MPN
Sbjct: 241 VYSAMKKVGYGDVDIVVAETGWPSAGDPNQAGVSMENAISYNRNLVKHVNSGKGTPLMPN 300

Query: 301 RTFETYVFSLFNENLKPSVSERNYGLFKPDLTPVYDVGILRNSPVNPFFIYLNPLASAGI 360
           RTFETY+FSLFNENLKPS SERNYGLF+PD TPVYDVGIL+N                  
Sbjct: 301 RTFETYIFSLFNENLKPSTSERNYGLFQPDFTPVYDVGILQNP----------------- 360

Query: 361 MPNPTTGPTAPAGQAPQPSDGGRKWCVPKTDASDEALQKNIDYVCSSGVDCGPIQAGGAC 420
              P  GPT+P G  P+PSD  R+WCVPKTDASDEALQKNIDYVCSSGVDCGPIQ GGAC
Sbjct: 361 ---PAMGPTSPVG-GPKPSDEEREWCVPKTDASDEALQKNIDYVCSSGVDCGPIQEGGAC 420

Query: 421 YDPNTVRSHASYAMDAYFQSSGRHDFNCDFNHTGVLTSTDPSYKACSYPLGAEKLTEKSV 480
           YDPNTVRSHASYAM+AYFQ++GRH+FNCDFNHT +LTSTDPSY+ACSYP   EKLTEKSV
Sbjct: 421 YDPNTVRSHASYAMNAYFQTAGRHEFNCDFNHTAILTSTDPSYEACSYPFDEEKLTEKSV 480

Query: 481 ASAAAMRRRIRLLGAVARTCIAVVAGGVLLI 512
           AS A +R R+    A+AR CIAVVAGGVLLI
Sbjct: 481 ASGATIRCRMVGAVALARACIAVVAGGVLLI 489

BLAST of Carg12372-RA vs. TrEMBL
Match: tr|A0A1S3C6X2|A0A1S3C6X2_CUCME (glucan endo-1,3-beta-glucosidase OS=Cucumis melo OX=3656 GN=LOC103497159 PE=3 SV=1)

HSP 1 Score: 820.5 bits (2118), Expect = 2.2e-234
Identity = 415/516 (80.43%), Postives = 450/516 (87.21%), Query Frame = 0

Query: 1   MANSLLLSLL-LFSLFLRLSFATYSIGVNYGTVADNLPPPSQVAAFLKSHTTIDRVKIFD 60
           MA SL LSLL      + LS A YSIGVNYGTVA+NLPPP+QVAAFLKS T+IDRVKIFD
Sbjct: 1   MAYSLFLSLLXXXXXXIPLSSAAYSIGVNYGTVANNLPPPTQVAAFLKSRTSIDRVKIFD 60

Query: 61  ANPDILRAFAATGIALTVSVANGDIPALAKLPAAQSWIANNILPFHPNTLINHIAVGNEI 120
           ANPDILRAFA TGIALTVS+ANGDIPALAKLPAAQSW+ANNILPFHP TLINHIAVGNEI
Sbjct: 61  ANPDILRAFAGTGIALTVSIANGDIPALAKLPAAQSWVANNILPFHPTTLINHIAVGNEI 120

Query: 121 LATSDKNLIAHMLPAMKALHSALTLANITDIKVSSPHSLGILSASEPPSTGRFRKGYDRA 180
           LATSDKNLIAH+LPAMKA+HSAL LANI+D+KVS+PHSLGILSASEPPSTGRFR+GYDRA
Sbjct: 121 LATSDKNLIAHLLPAMKAIHSALKLANISDVKVSTPHSLGILSASEPPSTGRFRRGYDRA 180

Query: 181 IFAPILEFHRKTKTPFMVNPYPFFGFRPATLDYALFKPNAGFFDNTTGKHYTNMFDAQMD 240
           IFAPIL+FH +TKTPFMVNPYPFFGFRPATLDYALFKPNAG FDN TGKHYTNMFDAQ+D
Sbjct: 181 IFAPILDFHNQTKTPFMVNPYPFFGFRPATLDYALFKPNAGVFDNATGKHYTNMFDAQLD 240

Query: 241 AVHAAMNKLGYGDVDIVVAETGWPSAGDPNQPGVSLENAISYNQNLVKHVNSGKGTPMMP 300
           AVH+AM K+G+GDVDIVVAETGWPSAGDPNQPGVS+ENAISYN+NLVKHVNSGKGTP+MP
Sbjct: 241 AVHSAMKKVGFGDVDIVVAETGWPSAGDPNQPGVSMENAISYNRNLVKHVNSGKGTPLMP 300

Query: 301 NRTFETYVFSLFNENLKPSVSERNYGLFKPDLTPVYDVGILRNSPVNPFFIYLNPLASAG 360
           NRTFETY+FSLFNENLKP+ SERNYGLF+PD TPVYDVGILRN                 
Sbjct: 301 NRTFETYIFSLFNENLKPTTSERNYGLFQPDFTPVYDVGILRNP---------------- 360

Query: 361 IMPNPTTGPTAPAGQAPQPSDGGRKWCVPKTDASDEALQKNIDYVCSSGVDCGPIQAGGA 420
               PT GPT+P G  P+PSD GR+WCVPKTDASDEALQKNIDYVCSSGVDCGPIQ GGA
Sbjct: 361 ----PTMGPTSPVG-GPKPSDEGRRWCVPKTDASDEALQKNIDYVCSSGVDCGPIQEGGA 420

Query: 421 CYDPNTVRSHASYAMDAYFQSSGRHDFNCDFNHTGVLTSTDPSYKACSYPLGAEKLTEKS 480
           CYDPNTVRSHASYAM+AYFQ++GRHDFNCDFNHT +LT TDPSY+ACSYP   EKL EKS
Sbjct: 421 CYDPNTVRSHASYAMNAYFQTAGRHDFNCDFNHTAILTFTDPSYEACSYPFDEEKLAEKS 480

Query: 481 VASAAAMR----RRIRLLGAVARTCIAVVAGGVLLI 512
           VASAA +R      + +  AVAR  IAVVAGGVLLI
Sbjct: 481 VASAATIRCSMVGAVAVAVAVARAFIAVVAGGVLLI 495

BLAST of Carg12372-RA vs. TrEMBL
Match: tr|A0A2C9WLS5|A0A2C9WLS5_MANES (Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_01G173700 PE=3 SV=1)

HSP 1 Score: 723.8 bits (1867), Expect = 2.7e-205
Identity = 352/476 (73.95%), Postives = 403/476 (84.66%), Query Frame = 0

Query: 6   LLSLLLFSLFLRLSFATYSIGVNYGTVADNLPPPSQVAAFLKSHTTIDRVKIFDANPDIL 65
           L   L F L  RLS A YS+GVNYGTVA+NLPPP+QVA+FLK+ TTIDR+KIFDANPDIL
Sbjct: 15  LFLFLTFHLLHRLSTAAYSVGVNYGTVANNLPPPTQVASFLKTQTTIDRIKIFDANPDIL 74

Query: 66  RAFAATGIALTVSVANGDIPALAKLPAAQSWIANNILPFHPNTLINHIAVGNEILATSDK 125
           RAFA TGI +TV+V NGDIP+LAKLPAAQ+WIANNILPFHP T+I +IAVGNEILATSDK
Sbjct: 75  RAFANTGILVTVTVGNGDIPSLAKLPAAQAWIANNILPFHPKTIIKYIAVGNEILATSDK 134

Query: 126 NLIAHMLPAMKALHSALTLANITDIKVSSPHSLGILSASEPPSTGRFRKGYDRAIFAPIL 185
           +LIAH LPAMKAL SAL LAN+T+I+VS+PHSLGILS+SEPPSTGRFRKGYD+ IFAPIL
Sbjct: 135 SLIAHTLPAMKALKSALELANVTNIQVSTPHSLGILSSSEPPSTGRFRKGYDQRIFAPIL 194

Query: 186 EFHRKTKTPFMVNPYPFFGFRPATLDYALFKPNAGFFDNTTGKHYTNMFDAQMDAVHAAM 245
           +FHRKTK+PFMVNPYP+FGFRP TL+YALF+PNAG FD  TGK+YTNMFDAQ+DAV++AM
Sbjct: 195 DFHRKTKSPFMVNPYPYFGFRPETLNYALFRPNAGVFDPATGKNYTNMFDAQLDAVYSAM 254

Query: 246 NKLGYGDVDIVVAETGWPSAGDPNQPGVSLENAISYNQNLVKHVNSGKGTPMMPNRTFET 305
            +LGY DVDIVVAETGWPS GDPNQPGVS+ENA+SYN NLVKHVNS KGTP+MPNRTFET
Sbjct: 255 KRLGYEDVDIVVAETGWPSVGDPNQPGVSMENALSYNGNLVKHVNSRKGTPLMPNRTFET 314

Query: 306 YVFSLFNENLKPSVSERNYGLFKPDLTPVYDVGILRNSPVNPFFIYLNPLASAGIMPNPT 365
           Y+FSLFNENLKP +SE+N+GLFKPDLTPVYDVG+LRN+                      
Sbjct: 315 YIFSLFNENLKPGISEQNFGLFKPDLTPVYDVGVLRNAQAK------------------- 374

Query: 366 TGPTAPAGQAPQPSDGGRKWCVPKTDASDEALQKNIDYVCSSGVDCGPIQAGGACYDPNT 425
            GPT+        S   RKWCVP++DASD ALQKNIDY CSSG++C PIQAGG C+DPNT
Sbjct: 375 -GPTSGTRAVAPSSSPDRKWCVPRSDASDAALQKNIDYACSSGINCRPIQAGGPCFDPNT 434

Query: 426 VRSHASYAMDAYFQSSGRHDFNCDFNHTGVLTSTDPSYKACSYPLGAEKLTEKSVA 482
           VRSHASYAM+AY+QS GR DFNCDFNHTGV+T+TDPSY+ACSYP   +K+ EKSVA
Sbjct: 435 VRSHASYAMNAYYQSFGRQDFNCDFNHTGVVTTTDPSYEACSYPFSDQKMEEKSVA 470

BLAST of Carg12372-RA vs. TrEMBL
Match: tr|W9RU41|W9RU41_9ROSA (Glucan endo-1,3-beta-glucosidase OS=Morus notabilis OX=981085 GN=L484_025235 PE=3 SV=1)

HSP 1 Score: 715.7 bits (1846), Expect = 7.5e-203
Identity = 348/476 (73.11%), Postives = 399/476 (83.82%), Query Frame = 0

Query: 10  LLFSLFLRLSFATYSIGVNYGTVADNLPPPSQVAAFLKSHTTIDRVKIFDANPDILRAFA 69
           + F  F       YS+GVNYGTVADNLPPPSQVA FLK+ TTIDRVKIFDANPDILRAFA
Sbjct: 12  IFFLFFFSFKATAYSVGVNYGTVADNLPPPSQVATFLKTRTTIDRVKIFDANPDILRAFA 71

Query: 70  ATGIALTVSVANGDIPALAKLPAAQSWIANNILPFHPNTLINHIAVGNEILATSDKNLIA 129
            TGIA+TV+V NGD+P+LAKLPAAQSW+ANNILPFHP T+IN IAVGNEI+ATSDK+LIA
Sbjct: 72  GTGIAVTVTVGNGDVPSLAKLPAAQSWVANNILPFHPKTIINSIAVGNEIVATSDKSLIA 131

Query: 130 HMLPAMKALHSALTLANITDIKVSSPHSLGILSA-SEPPSTGRFRKGYDRAIFAPILEFH 189
           H+LPAMKALHSAL LAN+T+++VS+PHSLGIL+  SEPPS+GRFR GYDR +FAPILEFH
Sbjct: 132 HLLPAMKALHSALKLANVTNVQVSTPHSLGILAPNSEPPSSGRFRSGYDRVVFAPILEFH 191

Query: 190 RKTKTPFMVNPYPFFGFRPATLDYALFKPNAGFFDNTTGKHYTNMFDAQMDAVHAAMNKL 249
           R+TK+PFM+NPYPFFGF   TL+YALFKPN G FDN TG +YTNMFDAQMDAV +AM K+
Sbjct: 192 RQTKSPFMINPYPFFGFSAKTLNYALFKPNGGVFDNVTGLNYTNMFDAQMDAVFSAMKKV 251

Query: 250 GYGDVDIVVAETGWPSAGDPNQPGVSLENAISYNQNLVKHVNSGKGTPMMPNRTFETYVF 309
           GY DVDIVVAETGWPSAGDPNQP V+LENA+SYN NLVKHV+SGKGTP+MPNRTFETY+F
Sbjct: 252 GYDDVDIVVAETGWPSAGDPNQPDVNLENALSYNGNLVKHVSSGKGTPLMPNRTFETYIF 311

Query: 310 SLFNENLKPSVSERNYGLFKPDLTPVYDVGILRNSPVNPFFIYLNPLASAGIMPNPTTGP 369
           SLFNENLKPS SERNYGLFKPDLTPVYDV +LRN                    +   GP
Sbjct: 312 SLFNENLKPSTSERNYGLFKPDLTPVYDVSVLRN--------------------DQALGP 371

Query: 370 TAPAGQAPQPSDGGRKWCVPKTDASDEALQKNIDYVCSSGVDCGPIQAGGACYDPNTVRS 429
           TA   QAP+  D  RKWCVP++DASDEALQKNIDYVCS+GVDC PIQ+GG C+DPNTVRS
Sbjct: 372 TADVAQAPKAVDTRRKWCVPRSDASDEALQKNIDYVCSAGVDCKPIQSGGPCFDPNTVRS 431

Query: 430 HASYAMDAYFQSSGRHDFNCDFNHTGVLTSTDPSYKACSYPLGAEKLTEKSVASAA 485
           HASYAM+AY+Q+ G  D+NCDF HTGV+T TDPSY+AC+YPL   KL ++SVA ++
Sbjct: 432 HASYAMNAYYQAFGPQDYNCDFGHTGVITDTDPSYQACTYPLEGVKL-QRSVAGSS 466

BLAST of Carg12372-RA vs. TrEMBL
Match: tr|A0A067KQC0|A0A067KQC0_JATCU (Uncharacterized protein OS=Jatropha curcas OX=180498 GN=JCGZ_11045 PE=3 SV=1)

HSP 1 Score: 712.2 bits (1837), Expect = 8.2e-202
Identity = 351/479 (73.28%), Postives = 402/479 (83.92%), Query Frame = 0

Query: 6   LLSLLLFSLFLRLSFATYSIGVNYGTVADNLPPPSQVAAFLKSHTTIDRVKIFDANPDIL 65
           LL LL F LFLR S A YSIGVNYGTVADNLP PSQVA FLK+ TTIDR+KIFDANPDIL
Sbjct: 8   LLLLLAFQLFLRSSTAAYSIGVNYGTVADNLPTPSQVANFLKTKTTIDRIKIFDANPDIL 67

Query: 66  RAFAATGIALTVSVANGDIPALAKLPAAQSWIANNILPFHPNTLINHIAVGNEILATSDK 125
           RAFA TGI++TV+V NGDIP+LAKLPAA++WIA NILP+HP T+I +IAVGNEILATSDK
Sbjct: 68  RAFANTGISVTVTVGNGDIPSLAKLPAAKAWIAANILPYHPKTIIKYIAVGNEILATSDK 127

Query: 126 NLIAHMLPAMKALHSALTLANITDIKVSSPHSLGILSASEPPSTGRFRKGYDRAIFAPIL 185
           +LIAH LPAMKAL SAL LANIT+I+VS+PHSLGILS+SEPPSTGRFRKGYD  IFAPIL
Sbjct: 128 SLIAHTLPAMKALKSALELANITNIQVSTPHSLGILSSSEPPSTGRFRKGYDTRIFAPIL 187

Query: 186 EFHRKTKTPFMVNPYPFFGFRPATLDYALFKPNAGFFDNTTGKHYTNMFDAQMDAVHAAM 245
           EFHR+TK+PFMVNPYPFFGF+  TL+YALFKPN G FD  TGK+YTNMFDAQ+DA+ +AM
Sbjct: 188 EFHRQTKSPFMVNPYPFFGFKAETLNYALFKPNGGVFDAATGKNYTNMFDAQLDAIFSAM 247

Query: 246 NKLGYGDVDIVVAETGWPSAGDPNQPGVSLENAISYNQNLVKHVNSGKGTPMMPNRTFET 305
            KLGY DVDIVVAETGWPS GDPNQPGVSLENA+SYN NLVKHVNSGKGTP+MPNRTFET
Sbjct: 248 KKLGYEDVDIVVAETGWPSVGDPNQPGVSLENAVSYNANLVKHVNSGKGTPLMPNRTFET 307

Query: 306 YVFSLFNENLKPSVSERNYGLFKPDLTPVYDVGILRNSPVNPFFIYLNPLASAGIMPNPT 365
           Y+F+LFNENLKP++SERN+GLFKPDL+PVYDVG+LRN   +                   
Sbjct: 308 YLFALFNENLKPTISERNFGLFKPDLSPVYDVGVLRNGQAH------------------- 367

Query: 366 TGPTAPAGQAPQPSDGGRKWCVPKTDASDEALQKNIDYVCSSGVDCGPIQAGGACYDPNT 425
            GPTA    A   S   R+WCVPK+DA DEALQKNIDYVCS+ VDC  IQAGG C+DPNT
Sbjct: 368 -GPTAATTAAAPSSSLDRRWCVPKSDAGDEALQKNIDYVCSNRVDCRAIQAGGPCFDPNT 427

Query: 426 VRSHASYAMDAYFQSSGRHDFNCDFNHTGVLTSTDPSYKACSYPLGAEKLTEKSVASAA 485
           VRSHASYAM+AY+Q+ GRHD+NCDFN +GVLT+ +PSY+AC+YP   +K+ E+SVA  +
Sbjct: 428 VRSHASYAMNAYYQTFGRHDYNCDFNGSGVLTTVNPSYEACNYPFDGQKMEERSVAGGS 466

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022948604.11.8e-27795.89glucan endo-1,3-beta-glucosidase-like [Cucurbita moschata][more]
XP_023522990.13.2e-27494.72glucan endo-1,3-beta-glucosidase-like [Cucurbita pepo subsp. pepo][more]
XP_022998328.19.3e-27494.52glucan endo-1,3-beta-glucosidase-like [Cucurbita maxima][more]
XP_011658156.11.0e-23581.21PREDICTED: glucan endo-1,3-beta-glucosidase [Cucumis sativus] >KGN65723.1 Glucan... [more]
XP_008457482.23.3e-23480.43PREDICTED: glucan endo-1,3-beta-glucosidase [Cucumis melo][more]
Match NameE-valueIdentityDescription
AT5G24318.17.2e-13550.96O-Glycosyl hydrolases family 17 protein[more]
AT3G55430.14.8e-13152.46O-Glycosyl hydrolases family 17 protein[more]
AT3G46570.11.2e-12666.37Glycosyl hydrolase superfamily protein[more]
AT4G34480.12.0e-11646.79O-Glycosyl hydrolases family 17 protein[more]
AT2G16230.11.7e-11545.59O-Glycosyl hydrolases family 17 protein[more]
Match NameE-valueIdentityDescription
sp|P52409|E13B_WHEAT8.1e-12851.11Glucan endo-1,3-beta-glucosidase OS=Triticum aestivum OX=4565 GN=GLC1 PE=2 SV=1[more]
sp|Q94G86|ALL9_OLEEU5.5e-11645.45Glucan endo-1,3-beta-D-glucosidase OS=Olea europaea OX=4146 GN=OLE9 PE=1 SV=1[more]
sp|Q9M069|E137_ARATH3.5e-11546.79Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 ... [more]
sp|Q8VYE5|E1312_ARATH4.5e-8639.24Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360... [more]
sp|Q9ZU91|E133_ARATH4.4e-8138.72Glucan endo-1,3-beta-glucosidase 3 OS=Arabidopsis thaliana OX=3702 GN=At2g01630 ... [more]
Match NameE-valueIdentityDescription
tr|A0A0A0LVC1|A0A0A0LVC1_CUCSA6.7e-23681.21Glucan endo-1,3-beta-glucosidase OS=Cucumis sativus OX=3659 GN=Csa_1G522000 PE=3... [more]
tr|A0A1S3C6X2|A0A1S3C6X2_CUCME2.2e-23480.43glucan endo-1,3-beta-glucosidase OS=Cucumis melo OX=3656 GN=LOC103497159 PE=3 SV... [more]
tr|A0A2C9WLS5|A0A2C9WLS5_MANES2.7e-20573.95Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_01G173700 PE=3 SV=... [more]
tr|W9RU41|W9RU41_9ROSA7.5e-20373.11Glucan endo-1,3-beta-glucosidase OS=Morus notabilis OX=981085 GN=L484_025235 PE=... [more]
tr|A0A067KQC0|A0A067KQC0_JATCU8.2e-20273.28Uncharacterized protein OS=Jatropha curcas OX=180498 GN=JCGZ_11045 PE=3 SV=1[more]
The following terms have been associated with this mRNA:
Vocabulary: Biological Process
TermDefinition
GO:0005975carbohydrate metabolic process
Vocabulary: Molecular Function
TermDefinition
GO:0004553hydrolase activity, hydrolyzing O-glycosyl compounds
Vocabulary: INTERPRO
TermDefinition
IPR017853Glycoside_hydrolase_SF
IPR000490Glyco_hydro_17
IPR012946X8
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0005575 cellular_component
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Carg12372Carg12372gene


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Carg12372-RACarg12372-RA-proteinpolypeptide


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg12372-RA.three_prime_UTR.1Carg12372-RA.three_prime_UTR.1three_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg12372-RA.CDS.3Carg12372-RA.CDS.3CDS
Carg12372-RA.CDS.2Carg12372-RA.CDS.2CDS
Carg12372-RA.CDS.1Carg12372-RA.CDS.1CDS


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg12372-RA.exon.3Carg12372-RA.exon.3exon
Carg12372-RA.exon.2Carg12372-RA.exon.2exon
Carg12372-RA.exon.1Carg12372-RA.exon.1exon


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012946X8 domainSMARTSM00768X8_clscoord: 384..468
e-value: 2.0E-46
score: 170.3
IPR012946X8 domainPFAMPF07983X8coord: 384..454
e-value: 1.5E-21
score: 76.7
IPR000490Glycoside hydrolase family 17PFAMPF00332Glyco_hydro_17coord: 25..337
e-value: 2.3E-86
score: 289.9
IPR000490Glycoside hydrolase family 17PROSITEPS00587GLYCOSYL_HYDROL_F17coord: 253..266
NoneNo IPR availableGENE3DG3DSA:3.20.20.80coord: 23..344
e-value: 1.0E-120
score: 404.4
NoneNo IPR availableGENE3DG3DSA:1.20.58.1040coord: 383..480
e-value: 2.1E-5
score: 26.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 362..383
NoneNo IPR availablePANTHERPTHR32227FAMILY NOT NAMEDcoord: 10..468
NoneNo IPR availablePANTHERPTHR32227:SF62GLUCOSIDASE-LIKE PROTEINcoord: 10..468
IPR017853Glycoside hydrolase superfamilySUPERFAMILYSSF51445(Trans)glycosidasescoord: 25..337