Carg12335-RA (mRNA) Silver-seed gourd

NameCarg12335-RA
TypemRNA
OrganismCucurbita argyrosperma (Silver-seed gourd)
Description3-ketoacyl-CoA synthase
LocationCucurbita_argyrosperma_scaffold_074 : 383466 .. 385230 (-)
Sequence length1599
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexonthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCCTCTTCCTCTCTCACATGGAGCCATTGGCATTGCTATACGCAATCCCCTTTGTGTATTTGGTACTCAAATTATGGAAGCATTTGGATGCGAAGAGGGACCAACAATGCTACATTTTGGACTACCAATGCTTCAAACCAACCGATGATAGAATGCTTGACACTCAGCTCTGCAGAGACCTTTTGATGCGCTCCAAAACTCTCGGCCTTGAAGAGTTCAAGTTCCTTCTCAAAGCCATTGTCAACTCCGGCCTCGGCGAGCAAACCTACGGCCCTAGTGTCGTCTTCTCCGCCACCGAACACTGCCCTTTGCTTCTCGACGCCATCTCCGAGATGGATGAGTTCTTCCACGACTCCCTTCAAAAGCTCTTCGACAAATCCCACGTTTCCCCCGACGAAATCGATATCCTCGTTGTCAACATCTCTTCCATGTCCACCTCCCCTTCTTTTGCCTCTAGAATCATCAACAGGTCTTTTATTTAACGATCGATACTATATGTTATCATCTCAAAAGTTACGATTTTCTTGAATCTTACGCGTTAAATTGGAATAGGTACAAAATGAGAGAAGACATTAAAGTGTTCAACATAAGTGGAATGGGATGCAGCGCGAGCTTAATCTCTATAGACATTGTGAGACGACTGTTCAAATCTTACAAGAACTCGTACGCGATCGTTGTCACCTCCGAGTCTCTAACTCCAAATTGGTATCTGGGTAACGACCGTTCGATGATTCTAGCCAACTGTTTGTTTCGAATCGGCGGCGCCGCCATACTCTTGACAAATAAAAGGAACATGAAGAACAAAGCCATGTTGAAGCTCAAGTGTCTTGTTAGAACCCACCATGGAGCTGTGGATGAGTCTTACGGTTGTTGCGTTCAAAAGGAAGACGACAAAGGAAACTTAGGTAAGAAGAAAATACGGGTTCATATCGTGACAGCCTCATTGAATAATTTGTTAACTTTTCACGAAAATTTTGATCTTTTTAAGGCATTTATCTGGGAAAGAACCTTCCAAAGGCAGCAACAAGTGCATTCATTGACAACCTAACAGAAATCTCCCCGCAAATCCTTCCCATAACAGAGATAATCCGGCTGACATTATCCAAAATAATCCACAAACTCTCCTCCACTGGCTCGTGGAAGCCGCAGCGGCCGGACTTCCGAACAGCGGCGGAGCATTTCTGCCTGCACACGGGAGGAAAGGCAGTGATAGATGGGGTTGGAAAGAGCTTGAGGCTGAGGGAGCATGATCTGGAGCCAGCCCGAATGACCCTTCACCGATTTGGGAACACATCGGCCAGCAGCCTGTGGTACGTGTTGGGCTACATGGAGGCCAAGAAGAGGCTGCAGAAGGGCGATAGGGTTCTTATGATAAGCTTTGGCGCAGGGTTTAAATGCAACAGCTGCTTGTGGGAAGTGACGGCGGCGGATTTGGGTGGTCGGAAGGGTAATGTGTGGGAAGATTGCATTAATAGCTACCCGCCGGTGTCTTTGGTTAACCCTTTCTTGGAAAGGTTCGGCTGGATTCAGAACCAAGATGCTTCCACTTTCAAGTCACTTTGATTCATACCTTTTTACTTTAAAATTTGAAAATTTCGAGATTAAAACGTCCGTTCTTAGCGGTAATAGATGTTTTATCTGTCTGTAGTGTAACCCGAGTACTATAAATTAAGCTATTTTATCTGTGGTAAAAGTTGTTTAATTAAAATTACCATACTCGAAATTTAATATATTATTAAGTGACATGTCAAATTATGAACT

mRNA sequence

ATGGCCCTCTTCCTCTCTCACATGGAGCCATTGGCATTGCTATACGCAATCCCCTTTGTGTATTTGGTACTCAAATTATGGAAGCATTTGGATGCGAAGAGGGACCAACAATGCTACATTTTGGACTACCAATGCTTCAAACCAACCGATGATAGAATGCTTGACACTCAGCTCTGCAGAGACCTTTTGATGCGCTCCAAAACTCTCGGCCTTGAAGAGTTCAAGTTCCTTCTCAAAGCCATTGTCAACTCCGGCCTCGGCGAGCAAACCTACGGCCCTAGTGTCGTCTTCTCCGCCACCGAACACTGCCCTTTGCTTCTCGACGCCATCTCCGAGATGGATGAGTTCTTCCACGACTCCCTTCAAAAGCTCTTCGACAAATCCCACGTTTCCCCCGACGAAATCGATATCCTCGTTGTCAACATCTCTTCCATGTCCACCTCCCCTTCTTTTGCCTCTAGAATCATCAACAGGTACAAAATGAGAGAAGACATTAAAGTGTTCAACATAAGTGGAATGGGATGCAGCGCGAGCTTAATCTCTATAGACATTGTGAGACGACTGTTCAAATCTTACAAGAACTCGTACGCGATCGTTGTCACCTCCGAGTCTCTAACTCCAAATTGGTATCTGGGTAACGACCGTTCGATGATTCTAGCCAACTGTTTGTTTCGAATCGGCGGCGCCGCCATACTCTTGACAAATAAAAGGAACATGAAGAACAAAGCCATGTTGAAGCTCAAGTGTCTTGTTAGAACCCACCATGGAGCTGTGGATGAGTCTTACGGTTGTTGCGTTCAAAAGGAAGACGACAAAGGAAACTTAGGCATTTATCTGGGAAAGAACCTTCCAAAGGCAGCAACAAGTGCATTCATTGACAACCTAACAGAAATCTCCCCGCAAATCCTTCCCATAACAGAGATAATCCGGCTGACATTATCCAAAATAATCCACAAACTCTCCTCCACTGGCTCGTGGAAGCCGCAGCGGCCGGACTTCCGAACAGCGGCGGAGCATTTCTGCCTGCACACGGGAGGAAAGGCAGTGATAGATGGGGTTGGAAAGAGCTTGAGGCTGAGGGAGCATGATCTGGAGCCAGCCCGAATGACCCTTCACCGATTTGGGAACACATCGGCCAGCAGCCTGTGGTACGTGTTGGGCTACATGGAGGCCAAGAAGAGGCTGCAGAAGGGCGATAGGGTTCTTATGATAAGCTTTGGCGCAGGGTTTAAATGCAACAGCTGCTTGTGGGAAGTGACGGCGGCGGATTTGGGTGGTCGGAAGGGTAATGTGTGGGAAGATTGCATTAATAGCTACCCGCCGGTGTCTTTGGTTAACCCTTTCTTGGAAAGGTTCGGCTGGATTCAGAACCAAGATGCTTCCACTTTCAAGTCACTTTGATTCATACCTTTTTACTTTAAAATTTGAAAATTTCGAGATTAAAACGTCCGTTCTTAGCGGTAATAGATGTTTTATCTGTCTGTAGTGTAACCCGAGTACTATAAATTAAGCTATTTTATCTGTGGTAAAAGTTGTTTAATTAAAATTACCATACTCGAAATTTAATATATTATTAAGTGACATGTCAAATTATGAACT

Coding sequence (CDS)

ATGGCCCTCTTCCTCTCTCACATGGAGCCATTGGCATTGCTATACGCAATCCCCTTTGTGTATTTGGTACTCAAATTATGGAAGCATTTGGATGCGAAGAGGGACCAACAATGCTACATTTTGGACTACCAATGCTTCAAACCAACCGATGATAGAATGCTTGACACTCAGCTCTGCAGAGACCTTTTGATGCGCTCCAAAACTCTCGGCCTTGAAGAGTTCAAGTTCCTTCTCAAAGCCATTGTCAACTCCGGCCTCGGCGAGCAAACCTACGGCCCTAGTGTCGTCTTCTCCGCCACCGAACACTGCCCTTTGCTTCTCGACGCCATCTCCGAGATGGATGAGTTCTTCCACGACTCCCTTCAAAAGCTCTTCGACAAATCCCACGTTTCCCCCGACGAAATCGATATCCTCGTTGTCAACATCTCTTCCATGTCCACCTCCCCTTCTTTTGCCTCTAGAATCATCAACAGGTACAAAATGAGAGAAGACATTAAAGTGTTCAACATAAGTGGAATGGGATGCAGCGCGAGCTTAATCTCTATAGACATTGTGAGACGACTGTTCAAATCTTACAAGAACTCGTACGCGATCGTTGTCACCTCCGAGTCTCTAACTCCAAATTGGTATCTGGGTAACGACCGTTCGATGATTCTAGCCAACTGTTTGTTTCGAATCGGCGGCGCCGCCATACTCTTGACAAATAAAAGGAACATGAAGAACAAAGCCATGTTGAAGCTCAAGTGTCTTGTTAGAACCCACCATGGAGCTGTGGATGAGTCTTACGGTTGTTGCGTTCAAAAGGAAGACGACAAAGGAAACTTAGGCATTTATCTGGGAAAGAACCTTCCAAAGGCAGCAACAAGTGCATTCATTGACAACCTAACAGAAATCTCCCCGCAAATCCTTCCCATAACAGAGATAATCCGGCTGACATTATCCAAAATAATCCACAAACTCTCCTCCACTGGCTCGTGGAAGCCGCAGCGGCCGGACTTCCGAACAGCGGCGGAGCATTTCTGCCTGCACACGGGAGGAAAGGCAGTGATAGATGGGGTTGGAAAGAGCTTGAGGCTGAGGGAGCATGATCTGGAGCCAGCCCGAATGACCCTTCACCGATTTGGGAACACATCGGCCAGCAGCCTGTGGTACGTGTTGGGCTACATGGAGGCCAAGAAGAGGCTGCAGAAGGGCGATAGGGTTCTTATGATAAGCTTTGGCGCAGGGTTTAAATGCAACAGCTGCTTGTGGGAAGTGACGGCGGCGGATTTGGGTGGTCGGAAGGGTAATGTGTGGGAAGATTGCATTAATAGCTACCCGCCGGTGTCTTTGGTTAACCCTTTCTTGGAAAGGTTCGGCTGGATTCAGAACCAAGATGCTTCCACTTTCAAGTCACTTTGA

Protein sequence

MALFLSHMEPLALLYAIPFVYLVLKLWKHLDAKRDQQCYILDYQCFKPTDDRMLDTQLCRDLLMRSKTLGLEEFKFLLKAIVNSGLGEQTYGPSVVFSATEHCPLLLDAISEMDEFFHDSLQKLFDKSHVSPDEIDILVVNISSMSTSPSFASRIINRYKMREDIKVFNISGMGCSASLISIDIVRRLFKSYKNSYAIVVTSESLTPNWYLGNDRSMILANCLFRIGGAAILLTNKRNMKNKAMLKLKCLVRTHHGAVDESYGCCVQKEDDKGNLGIYLGKNLPKAATSAFIDNLTEISPQILPITEIIRLTLSKIIHKLSSTGSWKPQRPDFRTAAEHFCLHTGGKAVIDGVGKSLRLREHDLEPARMTLHRFGNTSASSLWYVLGYMEAKKRLQKGDRVLMISFGAGFKCNSCLWEVTAADLGGRKGNVWEDCINSYPPVSLVNPFLERFGWIQNQDASTFKSL
BLAST of Carg12335-RA vs. NCBI nr
Match: XP_022949181.1 (3-ketoacyl-CoA synthase 12-like [Cucurbita moschata])

HSP 1 Score: 930.6 bits (2404), Expect = 2.0e-267
Identity = 455/459 (99.13%), Postives = 458/459 (99.78%), Query Frame = 0

Query: 8   MEPLALLYAIPFVYLVLKLWKHLDAKRDQQCYILDYQCFKPTDDRMLDTQLCRDLLMRSK 67
           MEPLALLYAIPFVYLVLKLWKHLDAKRDQQCYILDYQCFKPTDDRMLDTQLCRDLLMRSK
Sbjct: 1   MEPLALLYAIPFVYLVLKLWKHLDAKRDQQCYILDYQCFKPTDDRMLDTQLCRDLLMRSK 60

Query: 68  TLGLEEFKFLLKAIVNSGLGEQTYGPSVVFSATEHCPLLLDAISEMDEFFHDSLQKLFDK 127
           TLGLEEFKFLLKAIVNSGLGEQTYGPSVVFSATEHCPLLLDAISEMDEFFHDSLQKLFDK
Sbjct: 61  TLGLEEFKFLLKAIVNSGLGEQTYGPSVVFSATEHCPLLLDAISEMDEFFHDSLQKLFDK 120

Query: 128 SHVSPDEIDILVVNISSMSTSPSFASRIINRYKMREDIKVFNISGMGCSASLISIDIVRR 187
           SHVSPDEIDIL+VNISSMSTSPSFASRIINRYKMREDIKVFNISGMGCSASLISIDIVRR
Sbjct: 121 SHVSPDEIDILIVNISSMSTSPSFASRIINRYKMREDIKVFNISGMGCSASLISIDIVRR 180

Query: 188 LFKSYKNSYAIVVTSESLTPNWYLGNDRSMILANCLFRIGGAAILLTNKRNMKNKAMLKL 247
           LFKSYKNSYAIVVTSESLTPNWYLGNDRSMILANCLFRIGGAAILLTNKRNMKNKAMLKL
Sbjct: 181 LFKSYKNSYAIVVTSESLTPNWYLGNDRSMILANCLFRIGGAAILLTNKRNMKNKAMLKL 240

Query: 248 KCLVRTHHGAVDESYGCCVQKEDDKGNLGIYLGKNLPKAATSAFIDNLTEISPQILPITE 307
           KCLVRTHHGAVDESYGCCVQKEDDKGNLGIYLGKNLPKAATSAFIDNLTEISPQILPITE
Sbjct: 241 KCLVRTHHGAVDESYGCCVQKEDDKGNLGIYLGKNLPKAATSAFIDNLTEISPQILPITE 300

Query: 308 IIRLTLSKIIHKLSSTGSWKPQRPDFRTAAEHFCLHTGGKAVIDGVGKSLRLREHDLEPA 367
           IIRLTLSKIIH+LSSTGSWKPQRPDFRTAAEHFCLHTGGKAVIDGVGKSLRL EHDLEPA
Sbjct: 301 IIRLTLSKIIHQLSSTGSWKPQRPDFRTAAEHFCLHTGGKAVIDGVGKSLRLTEHDLEPA 360

Query: 368 RMTLHRFGNTSASSLWYVLGYMEAKKRLQKGDRVLMISFGAGFKCNSCLWEVTAADLGGR 427
           RMTLHRFGNTSASSLWYVLGYMEAKKRL+KGDRVLMISFGAGFKCNSCLWEVTAADLGGR
Sbjct: 361 RMTLHRFGNTSASSLWYVLGYMEAKKRLKKGDRVLMISFGAGFKCNSCLWEVTAADLGGR 420

Query: 428 KGNVWEDCINSYPPVSLVNPFLERFGWIQNQDASTFKSL 467
           KGNVWEDCINSYPPVSLVNPFLERFGWIQNQDASTFKSL
Sbjct: 421 KGNVWEDCINSYPPVSLVNPFLERFGWIQNQDASTFKSL 459

BLAST of Carg12335-RA vs. NCBI nr
Match: XP_022997816.1 (3-ketoacyl-CoA synthase 12-like [Cucurbita maxima])

HSP 1 Score: 920.2 bits (2377), Expect = 2.7e-264
Identity = 450/459 (98.04%), Postives = 455/459 (99.13%), Query Frame = 0

Query: 8   MEPLALLYAIPFVYLVLKLWKHLDAKRDQQCYILDYQCFKPTDDRMLDTQLCRDLLMRSK 67
           MEPL LLYAIPFVYLVLKLWKHLDAKRDQQCYILDYQCFKPTDDRMLDTQLCRDLLMRSK
Sbjct: 1   MEPLVLLYAIPFVYLVLKLWKHLDAKRDQQCYILDYQCFKPTDDRMLDTQLCRDLLMRSK 60

Query: 68  TLGLEEFKFLLKAIVNSGLGEQTYGPSVVFSATEHCPLLLDAISEMDEFFHDSLQKLFDK 127
           TLGLEEFKFLLKAIVNSGLGEQTYGPSVVFSATEHCPLLLDAISEMDEFFHDSLQKLFDK
Sbjct: 61  TLGLEEFKFLLKAIVNSGLGEQTYGPSVVFSATEHCPLLLDAISEMDEFFHDSLQKLFDK 120

Query: 128 SHVSPDEIDILVVNISSMSTSPSFASRIINRYKMREDIKVFNISGMGCSASLISIDIVRR 187
           SHVSPDEIDILVVNISSMSTSPSFASRIINRYKMREDIKVFNISGMGCSASLISIDIVRR
Sbjct: 121 SHVSPDEIDILVVNISSMSTSPSFASRIINRYKMREDIKVFNISGMGCSASLISIDIVRR 180

Query: 188 LFKSYKNSYAIVVTSESLTPNWYLGNDRSMILANCLFRIGGAAILLTNKRNMKNKAMLKL 247
           LFKSYKNSYAIVVTSESLTPNWYLGNDRSMILANCLFRIGGAAILLTNKRNMKNKAMLKL
Sbjct: 181 LFKSYKNSYAIVVTSESLTPNWYLGNDRSMILANCLFRIGGAAILLTNKRNMKNKAMLKL 240

Query: 248 KCLVRTHHGAVDESYGCCVQKEDDKGNLGIYLGKNLPKAATSAFIDNLTEISPQILPITE 307
           KCLVRTHHGAVDESYGCCVQKEDDKGNLGIYLGKNLPKAATSAFIDNLTEISP+ILPITE
Sbjct: 241 KCLVRTHHGAVDESYGCCVQKEDDKGNLGIYLGKNLPKAATSAFIDNLTEISPKILPITE 300

Query: 308 IIRLTLSKIIHKLSSTGSWKPQRPDFRTAAEHFCLHTGGKAVIDGVGKSLRLREHDLEPA 367
           IIRLTLSKIIH+LSSTGSWKPQRPDFRTAAEHFCLHTGGKAVI+GVGKSLRL E DLEPA
Sbjct: 301 IIRLTLSKIIHQLSSTGSWKPQRPDFRTAAEHFCLHTGGKAVINGVGKSLRLTERDLEPA 360

Query: 368 RMTLHRFGNTSASSLWYVLGYMEAKKRLQKGDRVLMISFGAGFKCNSCLWEVTAADLGGR 427
           RMTLHRFGNTSASSLWYVLGYMEAKKRL+KGDRVLMISFGAGFKCNSCLWEVTAADLGGR
Sbjct: 361 RMTLHRFGNTSASSLWYVLGYMEAKKRLKKGDRVLMISFGAGFKCNSCLWEVTAADLGGR 420

Query: 428 KGNVWEDCINSYPPVSLVNPFLERFGWIQNQDASTFKSL 467
           KGNVWEDCIN YPPVSLVNPFLERFGWIQN+DASTFKSL
Sbjct: 421 KGNVWEDCINGYPPVSLVNPFLERFGWIQNEDASTFKSL 459

BLAST of Carg12335-RA vs. NCBI nr
Match: XP_023524576.1 (3-ketoacyl-CoA synthase 12-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 871.7 bits (2251), Expect = 1.1e-249
Identity = 423/432 (97.92%), Postives = 431/432 (99.77%), Query Frame = 0

Query: 35  DQQCYILDYQCFKPTDDRMLDTQLCRDLLMRSKTLGLEEFKFLLKAIVNSGLGEQTYGPS 94
           DQQCYILDYQCFKPTDDRMLDTQLCRDLLMRSKTLGLEEFKFLLKA+VNSGLGEQTYGPS
Sbjct: 7   DQQCYILDYQCFKPTDDRMLDTQLCRDLLMRSKTLGLEEFKFLLKAVVNSGLGEQTYGPS 66

Query: 95  VVFSATEHCPLLLDAISEMDEFFHDSLQKLFDKSHVSPDEIDILVVNISSMSTSPSFASR 154
           VVFSATEHCPLLLDAISEMDEFFHDSLQKLFDKSHVSPD+IDILVVNISSMSTSPSFASR
Sbjct: 67  VVFSATEHCPLLLDAISEMDEFFHDSLQKLFDKSHVSPDKIDILVVNISSMSTSPSFASR 126

Query: 155 IINRYKMREDIKVFNISGMGCSASLISIDIVRRLFKSYKNSYAIVVTSESLTPNWYLGND 214
           IINRYKMR+DIKVFN+SGMGCSASLISIDIVRRLFKSYKNSYAIVVTSESLTPNWYLGND
Sbjct: 127 IINRYKMRDDIKVFNLSGMGCSASLISIDIVRRLFKSYKNSYAIVVTSESLTPNWYLGND 186

Query: 215 RSMILANCLFRIGGAAILLTNKRNMKNKAMLKLKCLVRTHHGAVDESYGCCVQKEDDKGN 274
           RSMILANCLFRIGGAAILLTNKRNMKN+AMLKLKCLVRTHHGAVDESYGCCVQKEDDKGN
Sbjct: 187 RSMILANCLFRIGGAAILLTNKRNMKNRAMLKLKCLVRTHHGAVDESYGCCVQKEDDKGN 246

Query: 275 LGIYLGKNLPKAATSAFIDNLTEISPQILPITEIIRLTLSKIIHKLSSTGSWKPQRPDFR 334
           LGIYLGKNLPKAATSAFIDNLTEISP+ILPITEIIRLTLSKIIHKLSSTGSWKPQRPDFR
Sbjct: 247 LGIYLGKNLPKAATSAFIDNLTEISPKILPITEIIRLTLSKIIHKLSSTGSWKPQRPDFR 306

Query: 335 TAAEHFCLHTGGKAVIDGVGKSLRLREHDLEPARMTLHRFGNTSASSLWYVLGYMEAKKR 394
           TAAEHFCLHTGGKAVI+GVGKSLRL EHDLEPARMTLHRFGNTSASSLWYVLGYMEAKKR
Sbjct: 307 TAAEHFCLHTGGKAVINGVGKSLRLTEHDLEPARMTLHRFGNTSASSLWYVLGYMEAKKR 366

Query: 395 LQKGDRVLMISFGAGFKCNSCLWEVTAADLGGRKGNVWEDCINSYPPVSLVNPFLERFGW 454
           L+KGDRVLMISFGAGFKCNSCLWEVTAADLGGRKGNVWEDCINSYPPVSLVNPFLERFGW
Sbjct: 367 LKKGDRVLMISFGAGFKCNSCLWEVTAADLGGRKGNVWEDCINSYPPVSLVNPFLERFGW 426

Query: 455 IQNQDASTFKSL 467
           IQNQDASTFKSL
Sbjct: 427 IQNQDASTFKSL 438

BLAST of Carg12335-RA vs. NCBI nr
Match: XP_022156169.1 (3-ketoacyl-CoA synthase 12-like, partial [Momordica charantia])

HSP 1 Score: 778.1 bits (2008), Expect = 1.7e-221
Identity = 374/451 (82.93%), Postives = 410/451 (90.91%), Query Frame = 0

Query: 20  VYLVLKLWKHLDAKRDQQCYILDYQCFKPTDDRMLDTQLCRDLLMRSKTLGLEEFKFLLK 79
           +YL++K+WKH DAKRDQQCYILDYQCFKPTDDRML T+LCRDL+MRSK LGLEEFKFLLK
Sbjct: 1   LYLLVKIWKHFDAKRDQQCYILDYQCFKPTDDRMLGTELCRDLMMRSKNLGLEEFKFLLK 60

Query: 80  AIVNSGLGEQTYGPSVVFSATEHCPLLLDAISEMDEFFHDSLQKLFDKSHVSPDEIDILV 139
           A+VNSG+GEQTYGP VVFSATEH P LLDAISEM+EFF DSLQKLFDKS VSP EIDILV
Sbjct: 61  AVVNSGIGEQTYGPGVVFSATEHRPSLLDAISEMEEFFQDSLQKLFDKSGVSPSEIDILV 120

Query: 140 VNISSMSTSPSFASRIINRYKMREDIKVFNISGMGCSASLISIDIVRRLFKSYKNSYAIV 199
           VNIS M+TSPSFASRIINRYKMRED+KVFN++GMGCSASLIS+D+VRR+FKSYK+SYAIV
Sbjct: 121 VNISLMTTSPSFASRIINRYKMREDVKVFNLTGMGCSASLISVDLVRRMFKSYKDSYAIV 180

Query: 200 VTSESLTPNWYLGNDRSMILANCLFRIGGAAILLTNKRNMKNKAMLKLKCLVRTHHGAVD 259
           VTSESLTPNWY GN+RSMILANCLFR GGAA+LLTNKR++KNKAM KLKCLVRTHHGA+D
Sbjct: 181 VTSESLTPNWYSGNERSMILANCLFRTGGAAVLLTNKRSLKNKAMFKLKCLVRTHHGALD 240

Query: 260 ESYGCCVQKEDDKGNLGIYLGKNLPKAATSAFIDNLTEISPQILPITEIIRLTLSKIIHK 319
           ES+GCCVQKEDD+GNLGIYLGKNLPKAAT AF DNL EISP ILPITEI RL L  I+HK
Sbjct: 241 ESFGCCVQKEDDQGNLGIYLGKNLPKAATRAFTDNLREISPNILPITEIFRLALLTILHK 300

Query: 320 LSSTGSWKPQRP----DFRTAAEHFCLHTGGKAVIDGVGKSLRLREHDLEPARMTLHRFG 379
           ++S GS K +RP    +FR  AEHFCLHTGGKAVIDGVGKSL L E+DLEPARMTLHRFG
Sbjct: 301 ITSAGSSKLRRPKPAANFRAGAEHFCLHTGGKAVIDGVGKSLSLTEYDLEPARMTLHRFG 360

Query: 380 NTSASSLWYVLGYMEAKKRLQKGDRVLMISFGAGFKCNSCLWEVTAADLGGRKGNVWEDC 439
           NTSASSLWYVLGYMEAKKRL+KGDR+LMISFGAGFKCNSC+WEV  AD GG  GNVWEDC
Sbjct: 361 NTSASSLWYVLGYMEAKKRLKKGDRILMISFGAGFKCNSCVWEV-VADSGGGAGNVWEDC 420

Query: 440 INSYPPVSLVNPFLERFGWIQNQDASTFKSL 467
           IN YPPVSL NPF+E+FGWIQNQD +TF SL
Sbjct: 421 INGYPPVSLANPFIEKFGWIQNQDPATFTSL 450

BLAST of Carg12335-RA vs. NCBI nr
Match: XP_022146108.1 (3-ketoacyl-CoA synthase 12-like [Momordica charantia])

HSP 1 Score: 773.1 bits (1995), Expect = 5.4e-220
Identity = 375/461 (81.34%), Postives = 414/461 (89.80%), Query Frame = 0

Query: 8   MEPLALLYAIPFVYLVLKLWKHLDAKRDQQCYILDYQCFKPTDDRMLDTQLCRDLLMRSK 67
           MEPL LL+A+P +YL++K+WKH DAKRDQQCYILDYQCFKPTDDRML T+LCRDL+MRSK
Sbjct: 1   MEPLVLLFALPLLYLLVKIWKHFDAKRDQQCYILDYQCFKPTDDRMLGTELCRDLMMRSK 60

Query: 68  TLGLEEFKFLLKAIVNSGLGEQTYGPSVVFSATEHCPLLLDAISEMDEFFHDSLQKLFDK 127
            LGLEEFKFLLKA+VNSG+GEQTYGP VVFSATEH P LLDAISEM+EFF DSLQKLFDK
Sbjct: 61  NLGLEEFKFLLKAVVNSGIGEQTYGPGVVFSATEHRPSLLDAISEMEEFFQDSLQKLFDK 120

Query: 128 SHVSPDEIDILVVNISSMSTSPSFASRIINRYKMREDIKVFNISGMGCSASLISIDIVRR 187
           S VSP EIDILVVNIS M+TSPSFASRIINRYKMRED+KVFN++GMGCSASLIS+D+VRR
Sbjct: 121 SGVSPSEIDILVVNISLMTTSPSFASRIINRYKMREDVKVFNLTGMGCSASLISVDLVRR 180

Query: 188 LFKSYKNSYAIVVTSESLTPNWYLGNDRSMILANCLFRIGGAAILLTNKRNMKNKAMLKL 247
           +FKSYK+SYAIVVTSESLTPNWY GN+RSMILANCLFR GGAA+LLTNKR++KNKAM KL
Sbjct: 181 MFKSYKDSYAIVVTSESLTPNWYSGNERSMILANCLFRTGGAAVLLTNKRSLKNKAMFKL 240

Query: 248 KCLVRTHHGAVDESYGCCVQKEDDKGNLGIYLGKNLPKAATSAFIDNLTEISPQILPITE 307
           KCLVRTHHGA+DES+GCCVQKEDD+GNLGIYLGKNLPKAAT AF DNL EISP ILPITE
Sbjct: 241 KCLVRTHHGALDESFGCCVQKEDDQGNLGIYLGKNLPKAATRAFTDNLREISPNILPITE 300

Query: 308 IIRLTLSKIIHKLSSTGSWKPQRP----DFRTAAEHFCLHTGGKAVIDGVGKSLRLREHD 367
           I RL L  I+HK++S GS K +RP    +FR  AEHFCLHTGGKAVIDGVGKSL L E+D
Sbjct: 301 IFRLALLTILHKITSAGSSKLRRPKPAANFRAGAEHFCLHTGGKAVIDGVGKSLSLTEYD 360

Query: 368 LEPARMTLHRFGNTSASSLWYVLGYMEAKKRLQKGDRVLMISFGAGFKCNSCLWEVTAAD 427
           LEPARMTLHRFGNTSASSLWYVL YMEAKKRL+KG+RVLMISFGAGFKCNSCLWEV   D
Sbjct: 361 LEPARMTLHRFGNTSASSLWYVLAYMEAKKRLRKGERVLMISFGAGFKCNSCLWEV-VRD 420

Query: 428 LGGRKGNVWEDCINSYPPVSLVNPFLERFGWIQNQDASTFK 465
           LG    NVW DCI+SYPP SL NPFLE++GWIQ +D +TFK
Sbjct: 421 LG--DANVWGDCIDSYPPESLANPFLEKYGWIQQEDIATFK 458

BLAST of Carg12335-RA vs. TAIR10
Match: AT2G28630.1 (3-ketoacyl-CoA synthase 12)

HSP 1 Score: 630.6 bits (1625), Expect = 7.9e-181
Identity = 299/470 (63.62%), Postives = 373/470 (79.36%), Query Frame = 0

Query: 8   MEPLALLYAIPFVYLVLKLWKHLDAKRDQQCYILDYQCFKPTDDRMLDTQLCRDLLMRSK 67
           M+ L L +++   YL  K+WK +D+K+D+ CYILDYQC KPTDDRM+ TQ   +++ R++
Sbjct: 1   MDLLFLFFSLLLSYLFFKIWKLIDSKQDKDCYILDYQCHKPTDDRMVSTQFSGEIIYRNQ 60

Query: 68  TLGLEEFKFLLKAIVNSGLGEQTYGPSVVFSATEHCPLLLDAISEMDEFFHDSLQKLFDK 127
            LGL E+KFLLKAIV+SG+GEQTY P +VF   E  P L D ISEM+EF+ DS+ KL ++
Sbjct: 61  NLGLTEYKFLLKAIVSSGIGEQTYAPRLVFEGREERPSLQDGISEMEEFYVDSIGKLLER 120

Query: 128 SHVSPDEIDILVVNISSMSTSPSFASRIINRYKMREDIKVFNISGMGCSASLISIDIVRR 187
           + +SP +IDILVVN+S +S++PS ASRIIN YKMR+D+KVFN++GMGCSASLIS+DIV+ 
Sbjct: 121 NQISPKDIDILVVNVSMLSSTPSLASRIINHYKMRDDVKVFNLTGMGCSASLISVDIVKN 180

Query: 188 LFKSYKNSYAIVVTSESLTPNWYLGNDRSMILANCLFRIGGAAILLTNKRNMKNKAMLKL 247
           +FKSY N  A+V TSESL+PNWY GN+RSMILANCLFR GG AILLTNKR+++ KAM KL
Sbjct: 181 IFKSYANKLALVATSESLSPNWYSGNNRSMILANCLFRSGGCAILLTNKRSLRKKAMFKL 240

Query: 248 KCLVRTHHGAVDESYGCCVQKEDDKGNLGIYLGKNLPKAATSAFIDNLTEISPQILPITE 307
           KC+VRTHHGA +ESY CC+Q ED++G +G YLGKNLPKAAT AF++NL  I+P+ILP+TE
Sbjct: 241 KCMVRTHHGAREESYNCCIQAEDEQGRVGFYLGKNLPKAATRAFVENLKVITPKILPVTE 300

Query: 308 IIRLTLSKIIHKLS-----STGSWK--PQRP-----DFRTAAEHFCLHTGGKAVIDGVGK 367
           +IR  L  +I K+      S GS    P  P     +F+T  EHFC+HTGGKAVIDG+G 
Sbjct: 301 LIRFMLKLLIKKIKIRQNPSKGSTNLPPGTPLKAGINFKTGIEHFCIHTGGKAVIDGIGH 360

Query: 368 SLRLREHDLEPARMTLHRFGNTSASSLWYVLGYMEAKKRLQKGDRVLMISFGAGFKCNSC 427
           SL L E+D+EPARMTLHRFGNTSASSLWYVL YMEAKKRL++GDRV MISFGAGFKCNSC
Sbjct: 361 SLDLNEYDIEPARMTLHRFGNTSASSLWYVLAYMEAKKRLKRGDRVFMISFGAGFKCNSC 420

Query: 428 LWEVTAADLGGR-KGNVWEDCINSYPPVSLVNPFLERFGWIQNQDASTFK 465
           +WEV     GG  KGNVW  CI+ YPP S++NP+LE+FGWIQ++D  TFK
Sbjct: 421 VWEVVRDLTGGESKGNVWNHCIDDYPPKSILNPYLEKFGWIQDEDPDTFK 470

BLAST of Carg12335-RA vs. TAIR10
Match: AT1G07720.1 (3-ketoacyl-CoA synthase 3)

HSP 1 Score: 609.4 bits (1570), Expect = 1.9e-174
Identity = 288/472 (61.02%), Postives = 371/472 (78.60%), Query Frame = 0

Query: 8   MEPLALLYAIPFVYLVLKLWKHLDAKRDQQCYILDYQCFKPTDDRMLDTQLCRDLLMRSK 67
           M+ L +L ++   YL+ K+WK +D+KRDQ CYILDYQC KP+DDRM++TQ   D+++R+K
Sbjct: 1   MDLLVMLLSLLVSYLIFKIWKRIDSKRDQNCYILDYQCHKPSDDRMVNTQFSGDIILRNK 60

Query: 68  TLGLEEFKFLLKAIVNSGLGEQTYGPSVVFSATEHCPLLLDAISEMDEFFHDSLQKLFDK 127
            L L E+KFLLKAIV+SG+GEQTY P + F   E  P L D +SEM+EF+ D+++K+  +
Sbjct: 61  HLRLNEYKFLLKAIVSSGIGEQTYAPRLFFEGREQRPTLQDGLSEMEEFYIDTIEKVLKR 120

Query: 128 SHVSPDEIDILVVNISSMSTSPSFASRIINRYKMREDIKVFNISGMGCSASLISIDIVRR 187
           + +SP EIDILVVN+S ++++PS ++RIIN YKMREDIKVFN++ MGCSAS+ISIDIV+ 
Sbjct: 121 NKISPSEIDILVVNVSMLNSTPSLSARIINHYKMREDIKVFNLTAMGCSASVISIDIVKN 180

Query: 188 LFKSYKNSYAIVVTSESLTPNWYLGNDRSMILANCLFRIGGAAILLTNKRNMKNKAMLKL 247
           +FK+YKN  A+VVTSESL+PNWY GN+RSMILANCLFR GG A+LLTNKR++  +AM KL
Sbjct: 181 IFKTYKNKLALVVTSESLSPNWYSGNNRSMILANCLFRSGGCAVLLTNKRSLSRRAMFKL 240

Query: 248 KCLVRTHHGAVDESYGCCVQKEDDKGNLGIYLGKNLPKAATSAFIDNLTEISPQILPITE 307
           +CLVRTHHGA D+S+  CVQKED+ G++G++L K LPKAAT AFIDNL  I+P+ILP+TE
Sbjct: 241 RCLVRTHHGARDDSFNACVQKEDELGHIGVHLDKTLPKAATRAFIDNLKVITPKILPVTE 300

Query: 308 IIRLTLSKIIHKLSST---GSWKPQRP----------DFRTAAEHFCLHTGGKAVIDGVG 367
           ++R  L  ++ KL S+   GS    +           +F+T  +HFC+HTGGKAVID +G
Sbjct: 301 LLRFMLCLLLKKLRSSPSKGSTNVTQAAPKAGVKAGINFKTGIDHFCIHTGGKAVIDAIG 360

Query: 368 KSLRLREHDLEPARMTLHRFGNTSASSLWYVLGYMEAKKRLQKGDRVLMISFGAGFKCNS 427
            SL L E+DLEPARMTLHRFGNTSASSLWYVLGYMEAKKRL++GDRV MISFGAGFKCNS
Sbjct: 361 YSLDLNEYDLEPARMTLHRFGNTSASSLWYVLGYMEAKKRLKRGDRVFMISFGAGFKCNS 420

Query: 428 CLWEVTA-ADLGGRKGNVWEDCINSYPPVSLVNPFLERFGWI-QNQDASTFK 465
           C+WEV    ++G   GNVW  CIN YPP S++NPF E++GWI + +D  TFK
Sbjct: 421 CVWEVVRDLNVGEAVGNVWNHCINQYPPKSILNPFFEKYGWIHEEEDPDTFK 472

BLAST of Carg12335-RA vs. TAIR10
Match: AT5G04530.1 (3-ketoacyl-CoA synthase 19)

HSP 1 Score: 474.6 bits (1220), Expect = 7.2e-134
Identity = 231/457 (50.55%), Postives = 324/457 (70.90%), Query Frame = 0

Query: 13  LLYAIPFVYLVLKLWKHLDAKRDQQCYILDYQCFKPTDDRMLDTQLCRDLLMRSKTLGLE 72
           LL +  FV+ + K       +  + CY+L Y+C+K  ++R LDT+ C  ++ R+K LGLE
Sbjct: 10  LLLSTLFVFYIFKF--VFKRRNQRNCYMLHYECYKGMEERKLDTETCAKVVQRNKNLGLE 69

Query: 73  EFKFLLKAIVNSGLGEQTYGPSVVFSATEHCPLLLDAISEMDEFFHDSLQKLFDKS--HV 132
           E++FLL+ + +SG+GE+TYGP  V    E  P LLDA SEMDE   D+L KLF K+   +
Sbjct: 70  EYRFLLRTMASSGIGEETYGPRNVLEGREDSPTLLDAHSEMDEIMFDTLDKLFHKTKGSI 129

Query: 133 SPDEIDILVVNISSMSTSPSFASRIINRYKMREDIKVFNISGMGCSASLISIDIVRRLFK 192
           SP +IDILVVN+S  + SPS  SR+INRYKMREDIK +N+SG+GCSAS+ISIDIV+R+F+
Sbjct: 130 SPSDIDILVVNVSLFAPSPSLTSRVINRYKMREDIKSYNLSGLGCSASVISIDIVQRMFE 189

Query: 193 SYKNSYAIVVTSESLTPNWYLGNDRSMILANCLFRIGGAAILLTNKRNMKNKAMLKLKCL 252
           + +N+ A+VV++E++ P+WY G DRSM+L+NCLFR GG+++LLTN    KN+A++KL  +
Sbjct: 190 TRENALALVVSTETMGPHWYCGKDRSMMLSNCLFRAGGSSVLLTNAARFKNQALMKLVTV 249

Query: 253 VRTHHGAVDESYGCCVQKEDDKGNLGIYLGKNLPKAATSAFIDNLTEISPQILPITEIIR 312
           VR H G+ DE+Y CC+Q ED  G+ G  L K L KAA  A   NL  + P++LP+ E+IR
Sbjct: 250 VRAHVGSDDEAYSCCIQMEDRDGHPGFLLTKYLKKAAARALTKNLQVLLPRVLPVKELIR 309

Query: 313 LTLSKIIHKLSSTGSWKPQRP---DFRTAAEHFCLHTGGKAVIDGVGKSLRLREHDLEPA 372
             + + + + +S            + +T  +HFC+H GG+A+I+GVGKSL L E D+EPA
Sbjct: 310 YAIVRALKRRTSAKREPASSGIGLNLKTGLQHFCIHPGGRAIIEGVGKSLGLTEFDIEPA 369

Query: 373 RMTLHRFGNTSASSLWYVLGYMEAKKRLQKGDRVLMISFGAGFKCNSCLWEVTAADLGGR 432
           RM LHRFGNTS+  LWYVLGYMEAK RL+KG+++LM+S GAGF+ N+C+WEV   DL  +
Sbjct: 370 RMALHRFGNTSSGGLWYVLGYMEAKNRLKKGEKILMMSMGAGFESNNCVWEV-LKDLDDK 429

Query: 433 KGNVWEDCINSYPPVSLV-NPFLERFGWIQNQDASTF 464
             NVWED ++ YP +S + NPF+E++ WI N D  +F
Sbjct: 430 --NVWEDSVDRYPELSRIPNPFVEKYDWI-NDDTMSF 460

BLAST of Carg12335-RA vs. TAIR10
Match: AT1G01120.1 (3-ketoacyl-CoA synthase 1)

HSP 1 Score: 368.2 bits (944), Expect = 7.3e-102
Identity = 182/428 (42.52%), Postives = 271/428 (63.32%), Query Frame = 0

Query: 14  LYAIPFVYLVLKLWKHLDAKRDQQCYILDYQCFKPTDDRMLDTQLCRDLLMRSKTLGLEE 73
           L  + F+  VL L+    A R +  Y++D+ C+KP D+R +       +   + +   + 
Sbjct: 101 LTCLVFLSFVLTLYV---ANRSKPVYLVDFSCYKPEDERKISVDSFLTMTEENGSFTDDT 160

Query: 74  FKFLLKAIVNSGLGEQTYGPSVVFSATEHCPLLLDAISEMDEFFHDSLQKLFDKSHVSPD 133
            +F  +    +GLG++TY P  + S       + +A +E +     +L  LF+K+ + P 
Sbjct: 161 VQFQQRISNRAGLGDETYLPRGITSTPPKLN-MSEARAEAEAVMFGALDSLFEKTGIKPA 220

Query: 134 EIDILVVNISSMSTSPSFASRIINRYKMREDIKVFNISGMGCSASLISIDIVRRLFKSYK 193
           E+ IL+VN S  + +PS ++ I+N YKMREDIK +N+ GMGCSA LISID+   L K+  
Sbjct: 221 EVGILIVNCSLFNPTPSLSAMIVNHYKMREDIKSYNLGGMGCSAGLISIDLANNLLKANP 280

Query: 194 NSYAIVVTSESLTPNWYLGNDRSMILANCLFRIGGAAILLTNKRNMKNKAMLKLKCLVRT 253
           NSYA+VV++E++T NWY GNDRSM+L NC+FR+GGAAILL+N+R  + K+   L  +VRT
Sbjct: 281 NSYAVVVSTENITLNWYFGNDRSMLLCNCIFRMGGAAILLSNRRQDRKKSKYSLVNVVRT 340

Query: 254 HHGAVDESYGCCVQKEDDKGNLGIYLGKNLPKAATSAFIDNLTEISPQILPITEIIRLTL 313
           H G+ D++Y C  QKED++G +G+ L + L   A  A   N+T + P +LP++E +   +
Sbjct: 341 HKGSDDKNYNCVYQKEDERGTIGVSLARELMSVAGDALKTNITTLGPMVLPLSEQLMFLI 400

Query: 314 SKIIHKLSSTGSWKPQRPDFRTAAEHFCLHTGGKAVIDGVGKSLRLREHDLEPARMTLHR 373
           S +  K+      KP  PDF+ A EHFC+H GG+AV+D V K+L L++  +EP+RMTLHR
Sbjct: 401 SLVKRKMFKL-KVKPYIPDFKLAFEHFCIHAGGRAVLDEVQKNLDLKDWHMEPSRMTLHR 460

Query: 374 FGNTSASSLWYVLGYMEAKKRLQKGDRVLMISFGAGFKCNSCLWE-VTAADLGGRKGNVW 433
           FGNTS+SSLWY + Y EAK R++ GDR+  I+FG+GFKCNS +W+ +         GN W
Sbjct: 461 FGNTSSSSLWYEMAYTEAKGRVKAGDRLWQIAFGSGFKCNSAVWKALRPVSTEEMTGNAW 520

Query: 434 EDCINSYP 441
              I+ YP
Sbjct: 521 AGSIDQYP 523

BLAST of Carg12335-RA vs. TAIR10
Match: AT2G16280.1 (3-ketoacyl-CoA synthase 9)

HSP 1 Score: 357.1 bits (915), Expect = 1.7e-98
Identity = 172/411 (41.85%), Postives = 267/411 (64.96%), Query Frame = 0

Query: 34  RDQQCYILDYQCFKPTDDRMLDTQLCRDLLMRSKTLGLEEFKFLLKAIVNSGLGEQTYGP 93
           R +  Y++DY C+ P +   +  Q   D     +       +F  K +  SGLGE+TY P
Sbjct: 104 RPRSVYLVDYSCYLPPESLQVKYQKFMDHSKLIEDFNESSLEFQRKILERSGLGEETYLP 163

Query: 94  SVVFSATEHC----PLLLDAISEMDEFFHDSLQKLFDKSHVSPDEIDILVVNISSMSTSP 153
             +     HC    P ++ A  E ++    +L KLF+ + ++P +I +LVVN S  + +P
Sbjct: 164 EAL-----HCIPPRPTMMAAREESEQVMFGALDKLFENTKINPRDIGVLVVNCSLFNPTP 223

Query: 154 SFASRIINRYKMREDIKVFNISGMGCSASLISIDIVRRLFKSYKNSYAIVVTSESLTPNW 213
           S ++ I+N+YK+R ++K FN+ GMGCSA +ISID+ + + + ++N+YA+VV++E++T NW
Sbjct: 224 SLSAMIVNKYKLRGNVKSFNLGGMGCSAGVISIDLAKDMLQVHRNTYAVVVSTENITQNW 283

Query: 214 YLGNDRSMILANCLFRIGGAAILLTNKRNMKNKAMLKLKCLVRTHHGAVDESYGCCVQKE 273
           Y GN ++M++ NCLFR+GG+AILL+NK   + ++  KL   VRTH GAV++++ C  Q++
Sbjct: 284 YFGNKKAMLIPNCLFRVGGSAILLSNKGKDRRRSKYKLVHTVRTHKGAVEKAFNCVYQEQ 343

Query: 274 DDKGNLGIYLGKNLPKAATSAFIDNLTEISPQILPITEIIRLTLSKIIHKLSSTGSWKPQ 333
           DD G  G+ L K+L   A  A   N+T + P +LPI+E I   ++ +  KL ++   KP 
Sbjct: 344 DDNGKTGVSLSKDLMAIAGEALKANITTLGPLVLPISEQILFFMTLVTKKLFNS-KLKPY 403

Query: 334 RPDFRTAAEHFCLHTGGKAVIDGVGKSLRLREHDLEPARMTLHRFGNTSASSLWYVLGYM 393
            PDF+ A +HFC+H GG+AVID + K+L+L +  +E +RMTLHRFGNTS+SS+WY L Y+
Sbjct: 404 IPDFKLAFDHFCIHAGGRAVIDELEKNLQLSQTHVEASRMTLHRFGNTSSSSIWYELAYI 463

Query: 394 EAKKRLQKGDRVLMISFGAGFKCNSCLWEVTAADLGGRKGNVWEDCINSYP 441
           EAK R++KG+RV  I+FG+GFKCNS +W V   ++     + WE CI+ YP
Sbjct: 464 EAKGRMKKGNRVWQIAFGSGFKCNSAVW-VALNNVKPSVSSPWEHCIDRYP 507

BLAST of Carg12335-RA vs. Swiss-Prot
Match: sp|Q9SIB2|KCS12_ARATH (3-ketoacyl-CoA synthase 12 OS=Arabidopsis thaliana OX=3702 GN=KCS12 PE=2 SV=1)

HSP 1 Score: 630.6 bits (1625), Expect = 1.4e-179
Identity = 299/470 (63.62%), Postives = 373/470 (79.36%), Query Frame = 0

Query: 8   MEPLALLYAIPFVYLVLKLWKHLDAKRDQQCYILDYQCFKPTDDRMLDTQLCRDLLMRSK 67
           M+ L L +++   YL  K+WK +D+K+D+ CYILDYQC KPTDDRM+ TQ   +++ R++
Sbjct: 1   MDLLFLFFSLLLSYLFFKIWKLIDSKQDKDCYILDYQCHKPTDDRMVSTQFSGEIIYRNQ 60

Query: 68  TLGLEEFKFLLKAIVNSGLGEQTYGPSVVFSATEHCPLLLDAISEMDEFFHDSLQKLFDK 127
            LGL E+KFLLKAIV+SG+GEQTY P +VF   E  P L D ISEM+EF+ DS+ KL ++
Sbjct: 61  NLGLTEYKFLLKAIVSSGIGEQTYAPRLVFEGREERPSLQDGISEMEEFYVDSIGKLLER 120

Query: 128 SHVSPDEIDILVVNISSMSTSPSFASRIINRYKMREDIKVFNISGMGCSASLISIDIVRR 187
           + +SP +IDILVVN+S +S++PS ASRIIN YKMR+D+KVFN++GMGCSASLIS+DIV+ 
Sbjct: 121 NQISPKDIDILVVNVSMLSSTPSLASRIINHYKMRDDVKVFNLTGMGCSASLISVDIVKN 180

Query: 188 LFKSYKNSYAIVVTSESLTPNWYLGNDRSMILANCLFRIGGAAILLTNKRNMKNKAMLKL 247
           +FKSY N  A+V TSESL+PNWY GN+RSMILANCLFR GG AILLTNKR+++ KAM KL
Sbjct: 181 IFKSYANKLALVATSESLSPNWYSGNNRSMILANCLFRSGGCAILLTNKRSLRKKAMFKL 240

Query: 248 KCLVRTHHGAVDESYGCCVQKEDDKGNLGIYLGKNLPKAATSAFIDNLTEISPQILPITE 307
           KC+VRTHHGA +ESY CC+Q ED++G +G YLGKNLPKAAT AF++NL  I+P+ILP+TE
Sbjct: 241 KCMVRTHHGAREESYNCCIQAEDEQGRVGFYLGKNLPKAATRAFVENLKVITPKILPVTE 300

Query: 308 IIRLTLSKIIHKLS-----STGSWK--PQRP-----DFRTAAEHFCLHTGGKAVIDGVGK 367
           +IR  L  +I K+      S GS    P  P     +F+T  EHFC+HTGGKAVIDG+G 
Sbjct: 301 LIRFMLKLLIKKIKIRQNPSKGSTNLPPGTPLKAGINFKTGIEHFCIHTGGKAVIDGIGH 360

Query: 368 SLRLREHDLEPARMTLHRFGNTSASSLWYVLGYMEAKKRLQKGDRVLMISFGAGFKCNSC 427
           SL L E+D+EPARMTLHRFGNTSASSLWYVL YMEAKKRL++GDRV MISFGAGFKCNSC
Sbjct: 361 SLDLNEYDIEPARMTLHRFGNTSASSLWYVLAYMEAKKRLKRGDRVFMISFGAGFKCNSC 420

Query: 428 LWEVTAADLGGR-KGNVWEDCINSYPPVSLVNPFLERFGWIQNQDASTFK 465
           +WEV     GG  KGNVW  CI+ YPP S++NP+LE+FGWIQ++D  TFK
Sbjct: 421 VWEVVRDLTGGESKGNVWNHCIDDYPPKSILNPYLEKFGWIQDEDPDTFK 470

BLAST of Carg12335-RA vs. Swiss-Prot
Match: sp|Q9LQP8|KCS3_ARATH (3-ketoacyl-CoA synthase 3 OS=Arabidopsis thaliana OX=3702 GN=KCS3 PE=2 SV=3)

HSP 1 Score: 609.4 bits (1570), Expect = 3.4e-173
Identity = 288/472 (61.02%), Postives = 371/472 (78.60%), Query Frame = 0

Query: 8   MEPLALLYAIPFVYLVLKLWKHLDAKRDQQCYILDYQCFKPTDDRMLDTQLCRDLLMRSK 67
           M+ L +L ++   YL+ K+WK +D+KRDQ CYILDYQC KP+DDRM++TQ   D+++R+K
Sbjct: 1   MDLLVMLLSLLVSYLIFKIWKRIDSKRDQNCYILDYQCHKPSDDRMVNTQFSGDIILRNK 60

Query: 68  TLGLEEFKFLLKAIVNSGLGEQTYGPSVVFSATEHCPLLLDAISEMDEFFHDSLQKLFDK 127
            L L E+KFLLKAIV+SG+GEQTY P + F   E  P L D +SEM+EF+ D+++K+  +
Sbjct: 61  HLRLNEYKFLLKAIVSSGIGEQTYAPRLFFEGREQRPTLQDGLSEMEEFYIDTIEKVLKR 120

Query: 128 SHVSPDEIDILVVNISSMSTSPSFASRIINRYKMREDIKVFNISGMGCSASLISIDIVRR 187
           + +SP EIDILVVN+S ++++PS ++RIIN YKMREDIKVFN++ MGCSAS+ISIDIV+ 
Sbjct: 121 NKISPSEIDILVVNVSMLNSTPSLSARIINHYKMREDIKVFNLTAMGCSASVISIDIVKN 180

Query: 188 LFKSYKNSYAIVVTSESLTPNWYLGNDRSMILANCLFRIGGAAILLTNKRNMKNKAMLKL 247
           +FK+YKN  A+VVTSESL+PNWY GN+RSMILANCLFR GG A+LLTNKR++  +AM KL
Sbjct: 181 IFKTYKNKLALVVTSESLSPNWYSGNNRSMILANCLFRSGGCAVLLTNKRSLSRRAMFKL 240

Query: 248 KCLVRTHHGAVDESYGCCVQKEDDKGNLGIYLGKNLPKAATSAFIDNLTEISPQILPITE 307
           +CLVRTHHGA D+S+  CVQKED+ G++G++L K LPKAAT AFIDNL  I+P+ILP+TE
Sbjct: 241 RCLVRTHHGARDDSFNACVQKEDELGHIGVHLDKTLPKAATRAFIDNLKVITPKILPVTE 300

Query: 308 IIRLTLSKIIHKLSST---GSWKPQRP----------DFRTAAEHFCLHTGGKAVIDGVG 367
           ++R  L  ++ KL S+   GS    +           +F+T  +HFC+HTGGKAVID +G
Sbjct: 301 LLRFMLCLLLKKLRSSPSKGSTNVTQAAPKAGVKAGINFKTGIDHFCIHTGGKAVIDAIG 360

Query: 368 KSLRLREHDLEPARMTLHRFGNTSASSLWYVLGYMEAKKRLQKGDRVLMISFGAGFKCNS 427
            SL L E+DLEPARMTLHRFGNTSASSLWYVLGYMEAKKRL++GDRV MISFGAGFKCNS
Sbjct: 361 YSLDLNEYDLEPARMTLHRFGNTSASSLWYVLGYMEAKKRLKRGDRVFMISFGAGFKCNS 420

Query: 428 CLWEVTA-ADLGGRKGNVWEDCINSYPPVSLVNPFLERFGWI-QNQDASTFK 465
           C+WEV    ++G   GNVW  CIN YPP S++NPF E++GWI + +D  TFK
Sbjct: 421 CVWEVVRDLNVGEAVGNVWNHCINQYPPKSILNPFFEKYGWIHEEEDPDTFK 472

BLAST of Carg12335-RA vs. Swiss-Prot
Match: sp|Q9LZ72|KCS19_ARATH (3-ketoacyl-CoA synthase 19 OS=Arabidopsis thaliana OX=3702 GN=KCS19 PE=2 SV=1)

HSP 1 Score: 474.6 bits (1220), Expect = 1.3e-132
Identity = 231/457 (50.55%), Postives = 324/457 (70.90%), Query Frame = 0

Query: 13  LLYAIPFVYLVLKLWKHLDAKRDQQCYILDYQCFKPTDDRMLDTQLCRDLLMRSKTLGLE 72
           LL +  FV+ + K       +  + CY+L Y+C+K  ++R LDT+ C  ++ R+K LGLE
Sbjct: 10  LLLSTLFVFYIFKF--VFKRRNQRNCYMLHYECYKGMEERKLDTETCAKVVQRNKNLGLE 69

Query: 73  EFKFLLKAIVNSGLGEQTYGPSVVFSATEHCPLLLDAISEMDEFFHDSLQKLFDKS--HV 132
           E++FLL+ + +SG+GE+TYGP  V    E  P LLDA SEMDE   D+L KLF K+   +
Sbjct: 70  EYRFLLRTMASSGIGEETYGPRNVLEGREDSPTLLDAHSEMDEIMFDTLDKLFHKTKGSI 129

Query: 133 SPDEIDILVVNISSMSTSPSFASRIINRYKMREDIKVFNISGMGCSASLISIDIVRRLFK 192
           SP +IDILVVN+S  + SPS  SR+INRYKMREDIK +N+SG+GCSAS+ISIDIV+R+F+
Sbjct: 130 SPSDIDILVVNVSLFAPSPSLTSRVINRYKMREDIKSYNLSGLGCSASVISIDIVQRMFE 189

Query: 193 SYKNSYAIVVTSESLTPNWYLGNDRSMILANCLFRIGGAAILLTNKRNMKNKAMLKLKCL 252
           + +N+ A+VV++E++ P+WY G DRSM+L+NCLFR GG+++LLTN    KN+A++KL  +
Sbjct: 190 TRENALALVVSTETMGPHWYCGKDRSMMLSNCLFRAGGSSVLLTNAARFKNQALMKLVTV 249

Query: 253 VRTHHGAVDESYGCCVQKEDDKGNLGIYLGKNLPKAATSAFIDNLTEISPQILPITEIIR 312
           VR H G+ DE+Y CC+Q ED  G+ G  L K L KAA  A   NL  + P++LP+ E+IR
Sbjct: 250 VRAHVGSDDEAYSCCIQMEDRDGHPGFLLTKYLKKAAARALTKNLQVLLPRVLPVKELIR 309

Query: 313 LTLSKIIHKLSSTGSWKPQRP---DFRTAAEHFCLHTGGKAVIDGVGKSLRLREHDLEPA 372
             + + + + +S            + +T  +HFC+H GG+A+I+GVGKSL L E D+EPA
Sbjct: 310 YAIVRALKRRTSAKREPASSGIGLNLKTGLQHFCIHPGGRAIIEGVGKSLGLTEFDIEPA 369

Query: 373 RMTLHRFGNTSASSLWYVLGYMEAKKRLQKGDRVLMISFGAGFKCNSCLWEVTAADLGGR 432
           RM LHRFGNTS+  LWYVLGYMEAK RL+KG+++LM+S GAGF+ N+C+WEV   DL  +
Sbjct: 370 RMALHRFGNTSSGGLWYVLGYMEAKNRLKKGEKILMMSMGAGFESNNCVWEV-LKDLDDK 429

Query: 433 KGNVWEDCINSYPPVSLV-NPFLERFGWIQNQDASTF 464
             NVWED ++ YP +S + NPF+E++ WI N D  +F
Sbjct: 430 --NVWEDSVDRYPELSRIPNPFVEKYDWI-NDDTMSF 460

BLAST of Carg12335-RA vs. Swiss-Prot
Match: sp|Q9MAM3|KCS1_ARATH (3-ketoacyl-CoA synthase 1 OS=Arabidopsis thaliana OX=3702 GN=KCS1 PE=1 SV=1)

HSP 1 Score: 368.2 bits (944), Expect = 1.3e-100
Identity = 182/428 (42.52%), Postives = 271/428 (63.32%), Query Frame = 0

Query: 14  LYAIPFVYLVLKLWKHLDAKRDQQCYILDYQCFKPTDDRMLDTQLCRDLLMRSKTLGLEE 73
           L  + F+  VL L+    A R +  Y++D+ C+KP D+R +       +   + +   + 
Sbjct: 101 LTCLVFLSFVLTLYV---ANRSKPVYLVDFSCYKPEDERKISVDSFLTMTEENGSFTDDT 160

Query: 74  FKFLLKAIVNSGLGEQTYGPSVVFSATEHCPLLLDAISEMDEFFHDSLQKLFDKSHVSPD 133
            +F  +    +GLG++TY P  + S       + +A +E +     +L  LF+K+ + P 
Sbjct: 161 VQFQQRISNRAGLGDETYLPRGITSTPPKLN-MSEARAEAEAVMFGALDSLFEKTGIKPA 220

Query: 134 EIDILVVNISSMSTSPSFASRIINRYKMREDIKVFNISGMGCSASLISIDIVRRLFKSYK 193
           E+ IL+VN S  + +PS ++ I+N YKMREDIK +N+ GMGCSA LISID+   L K+  
Sbjct: 221 EVGILIVNCSLFNPTPSLSAMIVNHYKMREDIKSYNLGGMGCSAGLISIDLANNLLKANP 280

Query: 194 NSYAIVVTSESLTPNWYLGNDRSMILANCLFRIGGAAILLTNKRNMKNKAMLKLKCLVRT 253
           NSYA+VV++E++T NWY GNDRSM+L NC+FR+GGAAILL+N+R  + K+   L  +VRT
Sbjct: 281 NSYAVVVSTENITLNWYFGNDRSMLLCNCIFRMGGAAILLSNRRQDRKKSKYSLVNVVRT 340

Query: 254 HHGAVDESYGCCVQKEDDKGNLGIYLGKNLPKAATSAFIDNLTEISPQILPITEIIRLTL 313
           H G+ D++Y C  QKED++G +G+ L + L   A  A   N+T + P +LP++E +   +
Sbjct: 341 HKGSDDKNYNCVYQKEDERGTIGVSLARELMSVAGDALKTNITTLGPMVLPLSEQLMFLI 400

Query: 314 SKIIHKLSSTGSWKPQRPDFRTAAEHFCLHTGGKAVIDGVGKSLRLREHDLEPARMTLHR 373
           S +  K+      KP  PDF+ A EHFC+H GG+AV+D V K+L L++  +EP+RMTLHR
Sbjct: 401 SLVKRKMFKL-KVKPYIPDFKLAFEHFCIHAGGRAVLDEVQKNLDLKDWHMEPSRMTLHR 460

Query: 374 FGNTSASSLWYVLGYMEAKKRLQKGDRVLMISFGAGFKCNSCLWE-VTAADLGGRKGNVW 433
           FGNTS+SSLWY + Y EAK R++ GDR+  I+FG+GFKCNS +W+ +         GN W
Sbjct: 461 FGNTSSSSLWYEMAYTEAKGRVKAGDRLWQIAFGSGFKCNSAVWKALRPVSTEEMTGNAW 520

Query: 434 EDCINSYP 441
              I+ YP
Sbjct: 521 AGSIDQYP 523

BLAST of Carg12335-RA vs. Swiss-Prot
Match: sp|Q9SIX1|KCS9_ARATH (3-ketoacyl-CoA synthase 9 OS=Arabidopsis thaliana OX=3702 GN=KCS9 PE=2 SV=1)

HSP 1 Score: 357.1 bits (915), Expect = 3.0e-97
Identity = 172/411 (41.85%), Postives = 267/411 (64.96%), Query Frame = 0

Query: 34  RDQQCYILDYQCFKPTDDRMLDTQLCRDLLMRSKTLGLEEFKFLLKAIVNSGLGEQTYGP 93
           R +  Y++DY C+ P +   +  Q   D     +       +F  K +  SGLGE+TY P
Sbjct: 104 RPRSVYLVDYSCYLPPESLQVKYQKFMDHSKLIEDFNESSLEFQRKILERSGLGEETYLP 163

Query: 94  SVVFSATEHC----PLLLDAISEMDEFFHDSLQKLFDKSHVSPDEIDILVVNISSMSTSP 153
             +     HC    P ++ A  E ++    +L KLF+ + ++P +I +LVVN S  + +P
Sbjct: 164 EAL-----HCIPPRPTMMAAREESEQVMFGALDKLFENTKINPRDIGVLVVNCSLFNPTP 223

Query: 154 SFASRIINRYKMREDIKVFNISGMGCSASLISIDIVRRLFKSYKNSYAIVVTSESLTPNW 213
           S ++ I+N+YK+R ++K FN+ GMGCSA +ISID+ + + + ++N+YA+VV++E++T NW
Sbjct: 224 SLSAMIVNKYKLRGNVKSFNLGGMGCSAGVISIDLAKDMLQVHRNTYAVVVSTENITQNW 283

Query: 214 YLGNDRSMILANCLFRIGGAAILLTNKRNMKNKAMLKLKCLVRTHHGAVDESYGCCVQKE 273
           Y GN ++M++ NCLFR+GG+AILL+NK   + ++  KL   VRTH GAV++++ C  Q++
Sbjct: 284 YFGNKKAMLIPNCLFRVGGSAILLSNKGKDRRRSKYKLVHTVRTHKGAVEKAFNCVYQEQ 343

Query: 274 DDKGNLGIYLGKNLPKAATSAFIDNLTEISPQILPITEIIRLTLSKIIHKLSSTGSWKPQ 333
           DD G  G+ L K+L   A  A   N+T + P +LPI+E I   ++ +  KL ++   KP 
Sbjct: 344 DDNGKTGVSLSKDLMAIAGEALKANITTLGPLVLPISEQILFFMTLVTKKLFNS-KLKPY 403

Query: 334 RPDFRTAAEHFCLHTGGKAVIDGVGKSLRLREHDLEPARMTLHRFGNTSASSLWYVLGYM 393
            PDF+ A +HFC+H GG+AVID + K+L+L +  +E +RMTLHRFGNTS+SS+WY L Y+
Sbjct: 404 IPDFKLAFDHFCIHAGGRAVIDELEKNLQLSQTHVEASRMTLHRFGNTSSSSIWYELAYI 463

Query: 394 EAKKRLQKGDRVLMISFGAGFKCNSCLWEVTAADLGGRKGNVWEDCINSYP 441
           EAK R++KG+RV  I+FG+GFKCNS +W V   ++     + WE CI+ YP
Sbjct: 464 EAKGRMKKGNRVWQIAFGSGFKCNSAVW-VALNNVKPSVSSPWEHCIDRYP 507

BLAST of Carg12335-RA vs. TrEMBL
Match: tr|A0A1S3C5N4|A0A1S3C5N4_CUCME (3-ketoacyl-CoA synthase OS=Cucumis melo OX=3656 GN=LOC103497181 PE=3 SV=1)

HSP 1 Score: 750.0 bits (1935), Expect = 3.3e-213
Identity = 366/463 (79.05%), Postives = 404/463 (87.26%), Query Frame = 0

Query: 8   MEPLALLYAIPFVYLVLKLWKHLDAKRDQQCYILDYQCFKPTDDRMLDTQLCRDLLMRSK 67
           ME L LLYAIP +YL LKLWK+ DAKRDQQCYILDYQCFKP+DDRML TQLCRDL+ R+K
Sbjct: 1   MELLFLLYAIPLLYLALKLWKYFDAKRDQQCYILDYQCFKPSDDRMLGTQLCRDLMKRTK 60

Query: 68  TLGLEEFKFLLKAIVNSGLGEQTYGPSVVFSATEHCPLLLDAISEMDEFFHDSLQKLFDK 127
            LGL+EFKFLLKA+VNSG+GEQTYGP VVFS  E  P L+D+I E++EFF D L KLF K
Sbjct: 61  NLGLDEFKFLLKAVVNSGIGEQTYGPRVVFSGKESHPSLVDSIVEVEEFFLDCLHKLFQK 120

Query: 128 SHVSPDEIDILVVNISSMSTSPSFASRIINRYKMREDIKVFNISGMGCSASLISIDIVRR 187
           S VSP +IDILVVNIS  STSPS AS IIN++KMREDI+VFN+SGMGCSASLIS+D+VRR
Sbjct: 121 SGVSPSQIDILVVNISMFSTSPSLASIIINKFKMREDIRVFNLSGMGCSASLISVDVVRR 180

Query: 188 LFKSYKNSYAIVVTSESLTPNWYLGNDRSMILANCLFRIGGAAILLTNKRNMKNKAMLKL 247
           +FKS+ NSY IVVTSESLT NWY GNDRSMIL+NCLFR GGAAILLTNKR++KNKAMLKL
Sbjct: 181 MFKSHMNSYGIVVTSESLTSNWYSGNDRSMILSNCLFRSGGAAILLTNKRSLKNKAMLKL 240

Query: 248 KCLVRTHHGAVDESYGCCVQKEDDKGNLGIYLGKNLPKAATSAFIDNLTEISPQILPITE 307
           KCLVRTHHGA DESYGCC QKEDDKGNLG +LGKNLP+AA  AF DNL EISP+ILPITE
Sbjct: 241 KCLVRTHHGAQDESYGCCYQKEDDKGNLGFHLGKNLPRAAIRAFTDNLREISPKILPITE 300

Query: 308 IIRLTLSKIIHKLSSTGSWKPQRP----DFRTAAEHFCLHTGGKAVIDGVGKSLRLREHD 367
           I RLT+  IIHK+S   S KP+ P    +F+T AEHFCLHTGGKAVIDGVGKSL L EHD
Sbjct: 301 IFRLTILTIIHKISLASSSKPRWPKPMANFKTGAEHFCLHTGGKAVIDGVGKSLNLTEHD 360

Query: 368 LEPARMTLHRFGNTSASSLWYVLGYMEAKKRLQKGDRVLMISFGAGFKCNSCLWEVTAAD 427
           LEPARMTLHRFGNTSASSLWYVLGYMEAKKRL+KGD+VLMISFGAGFKCNSCLWEV   +
Sbjct: 361 LEPARMTLHRFGNTSASSLWYVLGYMEAKKRLKKGDKVLMISFGAGFKCNSCLWEV-VTE 420

Query: 428 LGGRKGNVWEDCINSYPPVSLVNPFLERFGWIQNQDASTFKSL 467
           LG +KGNVWEDCIN YPPVSL NPFLE FGWIQN+DA+TFKSL
Sbjct: 421 LGEKKGNVWEDCINGYPPVSLANPFLESFGWIQNEDATTFKSL 462

BLAST of Carg12335-RA vs. TrEMBL
Match: tr|A0A0A0LUS6|A0A0A0LUS6_CUCSA (3-ketoacyl-CoA synthase OS=Cucumis sativus OX=3659 GN=Csa_1G501850 PE=3 SV=1)

HSP 1 Score: 748.8 bits (1932), Expect = 7.3e-213
Identity = 358/463 (77.32%), Postives = 407/463 (87.90%), Query Frame = 0

Query: 8   MEPLALLYAIPFVYLVLKLWKHLDAKRDQQCYILDYQCFKPTDDRMLDTQLCRDLLMRSK 67
           MEPL LLYAIP +YL  KLWKH DAKRDQQCYILDYQC+KP+DDRML TQLCRD++ R+K
Sbjct: 1   MEPLFLLYAIPLLYLAFKLWKHFDAKRDQQCYILDYQCYKPSDDRMLGTQLCRDVMKRTK 60

Query: 68  TLGLEEFKFLLKAIVNSGLGEQTYGPSVVFSATEHCPLLLDAISEMDEFFHDSLQKLFDK 127
            LGLEEFKFLLKA+VNSG+GEQTYGP+V+FS  E  P L+D+I E++EFF D L KLF+K
Sbjct: 61  VLGLEEFKFLLKAVVNSGIGEQTYGPNVIFSGKESHPSLVDSIVEVEEFFLDCLHKLFEK 120

Query: 128 SHVSPDEIDILVVNISSMSTSPSFASRIINRYKMREDIKVFNISGMGCSASLISIDIVRR 187
           S +SP +IDILVVNIS  STSPS AS +IN++KMREDIKVFN+SGMGCSASLIS+D++RR
Sbjct: 121 SGISPSQIDILVVNISMFSTSPSLASMLINKFKMREDIKVFNLSGMGCSASLISMDVMRR 180

Query: 188 LFKSYKNSYAIVVTSESLTPNWYLGNDRSMILANCLFRIGGAAILLTNKRNMKNKAMLKL 247
           +FKS+ NSY IVVTSESLTPNWY GNDRSMILANCLFR GGAAILLTNKR++KNKAMLKL
Sbjct: 181 MFKSHMNSYGIVVTSESLTPNWYSGNDRSMILANCLFRTGGAAILLTNKRSLKNKAMLKL 240

Query: 248 KCLVRTHHGAVDESYGCCVQKEDDKGNLGIYLGKNLPKAATSAFIDNLTEISPQILPITE 307
           KCLVRTHHGA DESY CC QKEDD+G+LGI+LGKNLP+AA  AF DNL EI+P+ILPITE
Sbjct: 241 KCLVRTHHGAQDESYDCCYQKEDDQGHLGIHLGKNLPRAAIRAFTDNLREIAPKILPITE 300

Query: 308 IIRLTLSKIIHKLSSTGSWKPQRP----DFRTAAEHFCLHTGGKAVIDGVGKSLRLREHD 367
           I RLT+  IIHK+S   S KP+ P    +F+T AEHFC+HTGGKAVIDGVGK L L EHD
Sbjct: 301 IFRLTILTIIHKISLASSSKPRWPKPMANFKTGAEHFCIHTGGKAVIDGVGKGLNLTEHD 360

Query: 368 LEPARMTLHRFGNTSASSLWYVLGYMEAKKRLQKGDRVLMISFGAGFKCNSCLWEVTAAD 427
           LEPARMTLHRFGNTSASSLWYVLGYMEAKKRL+KGD+VLMI+FGAGFKCNSCLWEV   +
Sbjct: 361 LEPARMTLHRFGNTSASSLWYVLGYMEAKKRLKKGDKVLMINFGAGFKCNSCLWEV-VRE 420

Query: 428 LGGRKGNVWEDCINSYPPVSLVNPFLERFGWIQNQDASTFKSL 467
           LGG+KGNVWEDCINSYPP+SL NPF+E FGWIQ +DA+TFKSL
Sbjct: 421 LGGKKGNVWEDCINSYPPLSLANPFMETFGWIQKEDATTFKSL 462

BLAST of Carg12335-RA vs. TrEMBL
Match: tr|A0A1S3C5T5|A0A1S3C5T5_CUCME (3-ketoacyl-CoA synthase OS=Cucumis melo OX=3656 GN=LOC103497180 PE=3 SV=1)

HSP 1 Score: 718.4 bits (1853), Expect = 1.0e-203
Identity = 349/463 (75.38%), Postives = 390/463 (84.23%), Query Frame = 0

Query: 8   MEPLALLYAIPFVYLVLKLWKHLDAKRDQQCYILDYQCFKPTDDRMLDTQLCRDLLMRSK 67
           MEPL LLYAIP +YL LKLWKH DAKRDQQCYILDYQC+KP+DDRML TQLCRDL+ R+K
Sbjct: 1   MEPLFLLYAIPLLYLALKLWKHFDAKRDQQCYILDYQCYKPSDDRMLGTQLCRDLMKRTK 60

Query: 68  TLGLEEFKFLLKAIVNSGLGEQTYGPSVVFSATEHCPLLLDAISEMDEFFHDSLQKLFDK 127
            LGLEEFKFLLKA+VNSG+GEQTYGP VVFS  E  P L+D+I E++EFF D LQKLF K
Sbjct: 61  NLGLEEFKFLLKAVVNSGIGEQTYGPHVVFSGKESHPSLVDSIVEVEEFFLDCLQKLFQK 120

Query: 128 SHVSPDEIDILVVNISSMSTSPSFASRIINRYKMREDIKVFNISGMGCSASLISIDIVRR 187
           S +SP +IDILVVNIS  STSPS AS IINR+KMREDIK                D++RR
Sbjct: 121 SGISPSQIDILVVNISMFSTSPSLASIIINRFKMREDIKXXXXXXXXXXXXXXXXDVMRR 180

Query: 188 LFKSYKNSYAIVVTSESLTPNWYLGNDRSMILANCLFRIGGAAILLTNKRNMKNKAMLKL 247
           +FKS+ NSY IVVTSESLTPNWY GNDRSMILANCLFR GGAAILLTNKRN+KNKAM KL
Sbjct: 181 MFKSHMNSYGIVVTSESLTPNWYSGNDRSMILANCLFRTGGAAILLTNKRNLKNKAMFKL 240

Query: 248 KCLVRTHHGAVDESYGCCVQKEDDKGNLGIYLGKNLPKAATSAFIDNLTEISPQILPITE 307
           KCL+RTHHGA +ESY CC QKEDD+GNLGI+LGKNLP+AA  AF DNL EI+P+ILPITE
Sbjct: 241 KCLIRTHHGAQNESYDCCYQKEDDQGNLGIHLGKNLPRAAIRAFTDNLREIAPKILPITE 300

Query: 308 IIRLTLSKIIHKLSSTGSWKPQRP----DFRTAAEHFCLHTGGKAVIDGVGKSLRLREHD 367
           I RLT+  IIHK+S   S KP+ P    +F+T AEHFCLHTGGKAVIDGVGKSL L EHD
Sbjct: 301 IFRLTILTIIHKISLASSSKPRWPKPMANFKTGAEHFCLHTGGKAVIDGVGKSLNLTEHD 360

Query: 368 LEPARMTLHRFGNTSASSLWYVLGYMEAKKRLQKGDRVLMISFGAGFKCNSCLWEVTAAD 427
           LEPARMTLHRFGNTSASSLWYVLGYMEAK+RL+KGD+V+MI+FGAGFKCNSCLWEV   +
Sbjct: 361 LEPARMTLHRFGNTSASSLWYVLGYMEAKRRLKKGDKVMMINFGAGFKCNSCLWEV-VRE 420

Query: 428 LGGRKGNVWEDCINSYPPVSLVNPFLERFGWIQNQDASTFKSL 467
           LG +KGNVWEDCIN YPPVSL NPF+E FGWIQN+DA+TFKSL
Sbjct: 421 LGEKKGNVWEDCINGYPPVSLANPFMESFGWIQNEDATTFKSL 462

BLAST of Carg12335-RA vs. TrEMBL
Match: tr|A0A0A0LXC4|A0A0A0LXC4_CUCSA (3-ketoacyl-CoA synthase OS=Cucumis sativus OX=3659 GN=Csa_1G501840 PE=3 SV=1)

HSP 1 Score: 678.7 bits (1750), Expect = 9.2e-192
Identity = 335/428 (78.27%), Postives = 373/428 (87.15%), Query Frame = 0

Query: 8   MEPLALLYAIPFVYLVLKLWKHLDAKRDQQCYILDYQCFKPTDDRMLDTQLCRDLLMRSK 67
           MEPL LLYAIP +YL LKLWKH DAKRDQQCYILDYQCFKP++DRML TQLCRDL+ R+K
Sbjct: 1   MEPLFLLYAIPLLYLALKLWKHFDAKRDQQCYILDYQCFKPSEDRMLGTQLCRDLMKRTK 60

Query: 68  TLGLEEFKFLLKAIVNSGLGEQTYGPSVVFSATEHCPLLLDAISEMDEFFHDSLQKLFDK 127
            LGLEEFKFLLKA+VNSG+GEQTYGP VVFS  E  P L+D+I E++EFF D L KLF K
Sbjct: 61  NLGLEEFKFLLKAVVNSGIGEQTYGPRVVFSGKESHPSLVDSIIEVEEFFLDCLHKLFQK 120

Query: 128 SHVSPDEIDILVVNISSMSTSPSFASRIINRYKMREDIKVFNISGMGCSASLISIDIVRR 187
           S VSP +IDILVVNIS +STSPS AS IIN++KM+EDIKVFN+SGMGCSASLIS+D+VRR
Sbjct: 121 SGVSPSQIDILVVNISMLSTSPSLASIIINKFKMKEDIKVFNLSGMGCSASLISVDVVRR 180

Query: 188 LFKSYKNSYAIVVTSESLTPNWYLGNDRSMILANCLFRIGGAAILLTNKRNMKNKAMLKL 247
           +FKS+ NSY IVVTSESLT NWY GNDRSMILANCLFR GGAAILLTNKR++KNKA+LKL
Sbjct: 181 MFKSHMNSYGIVVTSESLTANWYSGNDRSMILANCLFRSGGAAILLTNKRSLKNKALLKL 240

Query: 248 KCLVRTHHGAVDESYGCCVQKEDDKGNLGIYLGKNLPKAATSAFIDNLTEISPQILPITE 307
           KCLVRTHHGA DESY CC QKEDD+GNLG +LGK+LP+AA  AF DNL EISP+ILPITE
Sbjct: 241 KCLVRTHHGAQDESYECCYQKEDDQGNLGFHLGKSLPRAAIRAFTDNLREISPKILPITE 300

Query: 308 IIRLTLSKIIHKLSSTGSWKPQRP----DFRTAAEHFCLHTGGKAVIDGVGKSLRLREHD 367
           I RLT+  IIHK+S   S KP+ P    +F+T AEHFCLHTGGKAVIDGVGKSL L EHD
Sbjct: 301 IFRLTILTIIHKISLASSSKPRWPKPMANFKTGAEHFCLHTGGKAVIDGVGKSLNLTEHD 360

Query: 368 LEPARMTLHRFGNTSASSLWYVLGYMEAKKRLQKGDRVLMISFGAGFKCNSCLWEVTAAD 427
           LEPARMTLHRFGNTSASSLWYVLGYMEAKKRL+KGD+VLMISFGAGFKCNSCLWEV   +
Sbjct: 361 LEPARMTLHRFGNTSASSLWYVLGYMEAKKRLKKGDKVLMISFGAGFKCNSCLWEV-MTE 420

Query: 428 LGGRKGNV 432
           LG +KGNV
Sbjct: 421 LGEKKGNV 427

BLAST of Carg12335-RA vs. TrEMBL
Match: tr|F6GTU8|F6GTU8_VITVI (3-ketoacyl-CoA synthase OS=Vitis vinifera OX=29760 GN=VIT_06s0004g04000 PE=3 SV=1)

HSP 1 Score: 661.0 bits (1704), Expect = 2.0e-186
Identity = 313/459 (68.19%), Postives = 382/459 (83.22%), Query Frame = 0

Query: 8   MEPLALLYAIPFVYLVLKLWKHLDAKRDQQCYILDYQCFKPTDDRMLDTQLCRDLLMRSK 67
           ME +  L+ +P  YL   LWK +D KRDQ+CYILDYQC+KPTDDR L T+ C D++ R+K
Sbjct: 1   MEAMVYLFLLPVFYLFFLLWKFIDGKRDQECYILDYQCYKPTDDRKLSTEFCGDMIRRNK 60

Query: 68  TLGLEEFKFLLKAIVNSGLGEQTYGPSVVFSATEHCPLLLDAISEMDEFFHDSLQKLFDK 127
           +LGL+E+KFLLKAIVNSG+GE+TYGP  VFS  E  P +LD ISEMDEFF+DS++KL D+
Sbjct: 61  SLGLQEYKFLLKAIVNSGIGEETYGPRNVFSGNETHPSVLDGISEMDEFFNDSIEKLLDR 120

Query: 128 SHVSPDEIDILVVNISSMSTSPSFASRIINRYKMREDIKVFNISGMGCSASLISIDIVRR 187
             VSP EID LVVN+S ++T+PS A RIINRYKMRE+IK FN++GMGCSASLISI+IV+ 
Sbjct: 121 VGVSPSEIDALVVNVSMITTAPSLAGRIINRYKMRENIKAFNLTGMGCSASLISINIVQN 180

Query: 188 LFKSYKNSYAIVVTSESLTPNWYLGNDRSMILANCLFRIGGAAILLTNKRNMKNKAMLKL 247
           LFKSYKN YA+VVTSESL+PNWY GNDRSMIL+NCLFR GG A+LLTNKR ++++AMLKL
Sbjct: 181 LFKSYKNMYALVVTSESLSPNWYTGNDRSMILSNCLFRSGGCAVLLTNKRALRHRAMLKL 240

Query: 248 KCLVRTHHGAVDESYGCCVQKEDDKGNLGIYLGKNLPKAATSAFIDNLTEISPQILPITE 307
           +CLVRTHHGA DES+GCC+QKEDD+G LG +L K+LPKAAT AFIDNL  ISP+ILP+ E
Sbjct: 241 RCLVRTHHGAKDESFGCCIQKEDDQGRLGFFLNKSLPKAATRAFIDNLRVISPKILPVRE 300

Query: 308 IIRLTLSKIIHKL--SSTGSWKPQRPDFRTAAEHFCLHTGGKAVIDGVGKSLRLREHDLE 367
           ++R  ++ ++ K+  +S G  K    +F+T  +HFC+HTGGKAVIDG+GKSL L E+DLE
Sbjct: 301 LLRYMVASLVRKMYQNSKGGGKVV-INFKTGVDHFCIHTGGKAVIDGIGKSLELTEYDLE 360

Query: 368 PARMTLHRFGNTSASSLWYVLGYMEAKKRLQKGDRVLMISFGAGFKCNSCLWEVTAADLG 427
           PARMTLHRFGNTSASSLWYVL YM+ KKRL+KGDRVLMISFGAGFKCNSC+WEV A DL 
Sbjct: 361 PARMTLHRFGNTSASSLWYVLAYMDEKKRLKKGDRVLMISFGAGFKCNSCMWEV-ARDLD 420

Query: 428 GRKGNVWEDCINSYPPVSLVNPFLERFGWIQNQDASTFK 465
           G  GNVW+DCI+SYPP SL NPF+E++GWI   D +TF+
Sbjct: 421 G--GNVWKDCIDSYPPESLSNPFMEKYGWINQADENTFE 455

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022949181.12.0e-26799.133-ketoacyl-CoA synthase 12-like [Cucurbita moschata][more]
XP_022997816.12.7e-26498.043-ketoacyl-CoA synthase 12-like [Cucurbita maxima][more]
XP_023524576.11.1e-24997.923-ketoacyl-CoA synthase 12-like [Cucurbita pepo subsp. pepo][more]
XP_022156169.11.7e-22182.933-ketoacyl-CoA synthase 12-like, partial [Momordica charantia][more]
XP_022146108.15.4e-22081.343-ketoacyl-CoA synthase 12-like [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT2G28630.17.9e-18163.623-ketoacyl-CoA synthase 12[more]
AT1G07720.11.9e-17461.023-ketoacyl-CoA synthase 3[more]
AT5G04530.17.2e-13450.553-ketoacyl-CoA synthase 19[more]
AT1G01120.17.3e-10242.523-ketoacyl-CoA synthase 1[more]
AT2G16280.11.7e-9841.853-ketoacyl-CoA synthase 9[more]
Match NameE-valueIdentityDescription
sp|Q9SIB2|KCS12_ARATH1.4e-17963.623-ketoacyl-CoA synthase 12 OS=Arabidopsis thaliana OX=3702 GN=KCS12 PE=2 SV=1[more]
sp|Q9LQP8|KCS3_ARATH3.4e-17361.023-ketoacyl-CoA synthase 3 OS=Arabidopsis thaliana OX=3702 GN=KCS3 PE=2 SV=3[more]
sp|Q9LZ72|KCS19_ARATH1.3e-13250.553-ketoacyl-CoA synthase 19 OS=Arabidopsis thaliana OX=3702 GN=KCS19 PE=2 SV=1[more]
sp|Q9MAM3|KCS1_ARATH1.3e-10042.523-ketoacyl-CoA synthase 1 OS=Arabidopsis thaliana OX=3702 GN=KCS1 PE=1 SV=1[more]
sp|Q9SIX1|KCS9_ARATH3.0e-9741.853-ketoacyl-CoA synthase 9 OS=Arabidopsis thaliana OX=3702 GN=KCS9 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
tr|A0A1S3C5N4|A0A1S3C5N4_CUCME3.3e-21379.053-ketoacyl-CoA synthase OS=Cucumis melo OX=3656 GN=LOC103497181 PE=3 SV=1[more]
tr|A0A0A0LUS6|A0A0A0LUS6_CUCSA7.3e-21377.323-ketoacyl-CoA synthase OS=Cucumis sativus OX=3659 GN=Csa_1G501850 PE=3 SV=1[more]
tr|A0A1S3C5T5|A0A1S3C5T5_CUCME1.0e-20375.383-ketoacyl-CoA synthase OS=Cucumis melo OX=3656 GN=LOC103497180 PE=3 SV=1[more]
tr|A0A0A0LXC4|A0A0A0LXC4_CUCSA9.2e-19278.273-ketoacyl-CoA synthase OS=Cucumis sativus OX=3659 GN=Csa_1G501840 PE=3 SV=1[more]
tr|F6GTU8|F6GTU8_VITVI2.0e-18668.193-ketoacyl-CoA synthase OS=Vitis vinifera OX=29760 GN=VIT_06s0004g04000 PE=3 SV=... [more]
The following terms have been associated with this mRNA:
Vocabulary: Biological Process
TermDefinition
GO:0008152metabolic process
GO:0006633fatty acid biosynthetic process
Vocabulary: Molecular Function
TermDefinition
GO:0003824catalytic activity
GO:0016747transferase activity, transferring acyl groups other than amino-acyl groups
Vocabulary: Cellular Component
TermDefinition
GO:0016020membrane
Vocabulary: INTERPRO
TermDefinition
IPR0123923-ktacl-CoA_syn
IPR016039Thiolase-like
IPR013747ACP_syn_III_C
IPR013601FAE1_typ3_polyketide_synth
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006633 fatty acid biosynthetic process
biological_process GO:0008152 metabolic process
cellular_component GO:0016020 membrane
molecular_function GO:0003824 catalytic activity
molecular_function GO:0016747 transferase activity, transferring acyl groups other than amino-acyl groups

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Carg12335Carg12335gene


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Carg12335-RACarg12335-RA-proteinpolypeptide


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg12335-RA.three_prime_UTR.1Carg12335-RA.three_prime_UTR.1three_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg12335-RA.CDS.3Carg12335-RA.CDS.3CDS
Carg12335-RA.CDS.2Carg12335-RA.CDS.2CDS
Carg12335-RA.CDS.1Carg12335-RA.CDS.1CDS


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg12335-RA.exon.3Carg12335-RA.exon.3exon
Carg12335-RA.exon.2Carg12335-RA.exon.2exon
Carg12335-RA.exon.1Carg12335-RA.exon.1exon


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013601FAE1/Type III polyketide synthase-like proteinPFAMPF08392FAE1_CUT1_RppAcoord: 32..318
e-value: 1.0E-112
score: 376.1
IPR0137473-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III, C-terminalPFAMPF08541ACP_syn_III_Ccoord: 338..418
e-value: 2.0E-13
score: 50.2
IPR016039Thiolase-likeGENE3DG3DSA:3.40.47.10coord: 90..419
e-value: 2.9E-77
score: 262.2
IPR016039Thiolase-likeSUPERFAMILYSSF53901Thiolase-likecoord: 250..419
IPR016039Thiolase-likeSUPERFAMILYSSF53901Thiolase-likecoord: 27..238
IPR012392Very-long-chain 3-ketoacyl-CoA synthasePIRSFPIRSF036417Very_long_ch_3-ktacl-CoA_syncoord: 2..452
e-value: 4.1E-171
score: 567.7
IPR012392Very-long-chain 3-ketoacyl-CoA synthasePANTHERPTHR31561FAMILY NOT NAMEDcoord: 8..461
NoneNo IPR availablePANTHERPTHR31561:SF243-KETOACYL-COA SYNTHASE 12-RELATEDcoord: 8..461
NoneNo IPR availableCDDcd00831CHS_likecoord: 23..417
e-value: 8.04147E-109
score: 328.415