Carg12331-RA (mRNA) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg12331-RA
Type	mRNA
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	Actin depolymerizing factor-like protein
Location	Cucurbita_argyrosperma_scaffold_074 : 359359 .. 362041 (+)
Sequence length	1402

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TCAAACTCCGCTGTCGACGGCCATCGTGATATTGTGGAAAAAAGTAAAGCCATCGTTTACCAAGAAGAACATAGAAACAGAGAGAGGAGAATATCTCGAACATTTCGCGAATTTCTTCACAGAGCTAGAGAGAGAGAAATTGTTCTTCCCGTCTTCCGTTCCTCTCCCTAGATCTTCTGATTCACTTTCGCTACTCAAGATTCAGGCATGGTGAGACTCTTTTCACTGATTCTTCTTCATTTGCGGATTTCTTTATCTTTTGCTTTTTCGTATTTTTTCTTCTTGATTTAGTCTGTTTCTGTTCTTCTTGCTCTACTTGATCTGTGATGCCTGGTAGATTTGATTTTCCGGGCCTGCGGGGTGTGGATCTGTGTTATTCTATTTTTCTGATCCAGATCGTTTTAGTTTTCTTTTGTCCGATTTGCGCTGGATCGGTTTTGATTGATTTAGATTTCGTTTAGGATTGATTTCAGTATCTCGGAAATACTTGGCAATAGTTCGTTTCCTTGGATGTGATCGGGATTTTACTGATTGACGCCGTTCCTTAGTCATCCGAAATTGATAGTTTATATTTTTCTTTAGTTTGGTGTTTTCTTGTGCTTGGTTCTGTAGTGATTGAGATGATGTTTGGTGGTTGGTGGGAGGATGGCGATATTTTGCGTGTACTATCAGAATGGTAATATTGTGAAGTAGATCGAGTGCATGTGCTTGTTGAATATGGGGCCAATGATAATTGGTTGGCGGCAGCATAGTCAGTGGACCAAAGAAGCCCCCAACGGCCGAGGATAAATATAGGGAAGAATTGGAAATAAATTGGGTTTTTGATATTTTTATTGTGATGCCCACTTCTAATTTTCAATATTACATCAAATCTCGAATGCTAAAACCGCTCTCTTGAGAATCAAAATGTTATGCATTAGCCAATTGACAACTCATCCATACCAACCAATGAATGTTATCTCAATCATTGCGGAGAATTTGTGAAGAAGAAGAAGAATAGACTCTAAAAGGACCATGCGGAGATCATAATGATAATGGTTCTGTTACACTGTGATGATGATCATGCCTTCGGGGTCCCACATTGATCGGAGAAGGGAATGAGTGCCAGTGAGGATGTTGGATCCCAAAGGGCGTAGATTGTGAGACCCCACATCGATTGATGAGAAGAACCAAACATTTTTTATATGGGTGTGGAAATCTCTCTCTAACATACGTTTTAAAAACCTTGAGGGGAAGCTCGAAAGAGAAAACCCAAAGAGGACAATATCTGCTAGCGGTGGGCTTAGTTGTTATGCTTGTTATGATAACCTTTATAAGTATCTGATATTTCCTATGCCTAGTGTTCACATCTTTGAATTCTTTCCTCTTAAGCTGTATTATCATTTTCTTCCACATTCTTTTTCAGGCTAATGCGGCATCCGGTATGGCTGTACATGATGAATGTAAGCTCAAGTTCTTAGAGCTGAAAACCAAGAGAACTTACAGATACATTGTATATAAGATTGAAGAGAAGCAAAAGCAAGTGATTGTGGAAAAAGTTGGCGAACCAAGTCAAAGCTATGAAGATTTCACAGCTTGTCTTCCTGCTGATGAATGCCGATACGCGGTTTATGATTTTGACTTCCTCACTAAAGAGAATGTCCAGAAGAGCAGAATTTTCTTCATTGCTTGGTAATGTTACGATCAAAAAAGAAAAAAGAAAAACGTGTAGTTAGTTCTATCACTAATCATCACCATATTCTTTTGTTGTTGCAAAGGTCTCCGGACATATCAAAGGTAAGAAGCAAGATGATCTATGCAAGTTCAAAGGACAAGTTCAGGAGAGAATTGGATGGCATTCAAATTGAGCTGCAAGCTACTGACCCAACTGAAATGGGTCTCGATGTTTTCAACAGCCGGGCTAAATAAGAAGCCTCCTCCTGGTTATGAATTTCTTGTGGTGTCTGTTTTACATTGTGAAGTGATGATGTTATTCATTTCTGGGTTTATGAATGTGCTTTGGTTGATTACATATTGGGTTGCTTTGAGTTTTGTCTTGTAGAATTATGTCCATCTGGAACAACAAATCTTCGGTCATTCCACCATCACACCACCTTATATTGTATGATTTATCGTGTGCAATTAATGCAATATCTCCCTATGTAATGCTACATGATTTTCATCTTGTGTCATGCCAAGAACATGGGAGATGATGTTTGACCATGTTTAGCTCCGAGGATGCTAATGAGTTGCTGTTTTCAATGTCAATTTCACTGTTTCTGAGCAGCAGCATGGGCTTTATAACCAAGTTGGCTCAATTCTCATATGGTTGGTTCTCTTCTTTAATCCTTGTTTTCAATTACTTGCAGAATTTAGATCTCATCAACATTTTTGCATTGGTAAGCTAGAAGGATCCATAATGTTTCTCTGTTGATCTGTTTAGTGGAAGCATAAGGTTCTCAATATCTTCATGCCAGGCTTGGCTAAATTTGTTGTTTATGGGGCAATAGTGCTGTTTTTTTTTTCTTTCCCCATTTTGTGTGATGTTACTATCTGGCTGTTTTTCCTGAAATCATTACTCTAAATTTTCTCGTGCACTAATAATTCTACTCCCTCGACTACAATCACGTACCTATTTTTTCAAATCTGGAAGTACCTTATCGATAGTACCTTATCGATAGACCATGTTATCTAGACTTTCAAT

mRNA sequence

TCAAACTCCGCTGTCGACGGCCATCGTGATATTGTGGAAAAAAGTAAAGCCATCGTTTACCAAGAAGAACATAGAAACAGAGAGAGGAGAATATCTCGAACATTTCGCGAATTTCTTCACAGAGCTAGAGAGAGAGAAATTGTTCTTCCCGTCTTCCGTTCCTCTCCCTAGATCTTCTGATTCACTTTCGCTACTCAAGATTCAGGCATGGCTAATGCGGCATCCGGTATGGCTGTACATGATGAATGTAAGCTCAAGTTCTTAGAGCTGAAAACCAAGAGAACTTACAGATACATTGTATATAAGATTGAAGAGAAGCAAAAGCAAGTGATTGTGGAAAAAGTTGGCGAACCAAGTCAAAGCTATGAAGATTTCACAGCTTGTCTTCCTGCTGATGAATGCCGATACGCGGTTTATGATTTTGACTTCCTCACTAAAGAGAATGTCCAGAAGAGCAGAATTTTCTTCATTGCTTGGTCTCCGGACATATCAAAGGTAAGAAGCAAGATGATCTATGCAAGTTCAAAGGACAAGTTCAGGAGAGAATTGGATGGCATTCAAATTGAGCTGCAAGCTACTGACCCAACTGAAATGGGTCTCGATGTTTTCAACAGCCGGGCTAAATAAGAAGCCTCCTCCTGGTTATGAATTTCTTGTGGTGTCTGTTTTACATTGTGAAGTGATGATGTTATTCATTTCTGGGTTTATGAATGTGCTTTGGTTGATTACATATTGGGTTGCTTTGAGTTTTGTCTTGTAGAATTATGTCCATCTGGAACAACAAATCTTCGGTCATTCCACCATCACACCACCTTATATTGTATGATTTATCGTGTGCAATTAATGCAATATCTCCCTATGTAATGCTACATGATTTTCATCTTGTGTCATGCCAAGAACATGGGAGATGATGTTTGACCATGTTTAGCTCCGAGGATGCTAATGAGTTGCTGTTTTCAATGTCAATTTCACTGTTTCTGAGCAGCAGCATGGGCTTTATAACCAAGTTGGCTCAATTCTCATATGGTTGGTTCTCTTCTTTAATCCTTGTTTTCAATTACTTGCAGAATTTAGATCTCATCAACATTTTTGCATTGGTAAGCTAGAAGGATCCATAATGTTTCTCTGTTGATCTGTTTAGTGGAAGCATAAGGTTCTCAATATCTTCATGCCAGGCTTGGCTAAATTTGTTGTTTATGGGGCAATAGTGCTGTTTTTTTTTTCTTTCCCCATTTTGTGTGATGTTACTATCTGGCTGTTTTTCCTGAAATCATTACTCTAAATTTTCTCGTGCACTAATAATTCTACTCCCTCGACTACAATCACGTACCTATTTTTTCAAATCTGGAAGTACCTTATCGATAGTACCTTATCGATAGACCATGTTATCTAGACTTTCAAT

Coding sequence (CDS)

ATGGCTAATGCGGCATCCGGTATGGCTGTACATGATGAATGTAAGCTCAAGTTCTTAGAGCTGAAAACCAAGAGAACTTACAGATACATTGTATATAAGATTGAAGAGAAGCAAAAGCAAGTGATTGTGGAAAAAGTTGGCGAACCAAGTCAAAGCTATGAAGATTTCACAGCTTGTCTTCCTGCTGATGAATGCCGATACGCGGTTTATGATTTTGACTTCCTCACTAAAGAGAATGTCCAGAAGAGCAGAATTTTCTTCATTGCTTGGTCTCCGGACATATCAAAGGTAAGAAGCAAGATGATCTATGCAAGTTCAAAGGACAAGTTCAGGAGAGAATTGGATGGCATTCAAATTGAGCTGCAAGCTACTGACCCAACTGAAATGGGTCTCGATGTTTTCAACAGCCGGGCTAAATAA

Protein sequence

MANAASGMAVHDECKLKFLELKTKRTYRYIVYKIEEKQKQVIVEKVGEPSQSYEDFTACLPADECRYAVYDFDFLTKENVQKSRIFFIAWSPDISKVRSKMIYASSKDKFRRELDGIQIELQATDPTEMGLDVFNSRAK

Homology

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BLAST of Carg12331-RA vs. NCBI nr
Match: XP_022948707.1 (actin-depolymerizing factor 2-like [Cucurbita moschata] >XP_023521573.1 actin-depolymerizing factor 2-like [Cucurbita pepo subsp. pepo] >XP_023523187.1 actin-depolymerizing factor 2-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023523188.1 actin-depolymerizing factor 2-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 278.9 bits (712), Expect = 9.6e-72
Identity = 139/139 (100.00%), Postives = 139/139 (100.00%), Query Frame = 0


Query: 1   MANAASGMAVHDECKLKFLELKTKRTYRYIVYKIEEKQKQVIVEKVGEPSQSYEDFTACL 60
           MANAASGMAVHDECKLKFLELKTKRTYRYIVYKIEEKQKQVIVEKVGEPSQSYEDFTACL
Sbjct: 1   MANAASGMAVHDECKLKFLELKTKRTYRYIVYKIEEKQKQVIVEKVGEPSQSYEDFTACL 60

Query: 61  PADECRYAVYDFDFLTKENVQKSRIFFIAWSPDISKVRSKMIYASSKDKFRRELDGIQIE 120
           PADECRYAVYDFDFLTKENVQKSRIFFIAWSPDISKVRSKMIYASSKDKFRRELDGIQIE
Sbjct: 61  PADECRYAVYDFDFLTKENVQKSRIFFIAWSPDISKVRSKMIYASSKDKFRRELDGIQIE 120

Query: 121 LQATDPTEMGLDVFNSRAK 140
           LQATDPTEMGLDVFNSRAK
Sbjct: 121 LQATDPTEMGLDVFNSRAK 139

[Close]

BLAST of Carg12331-RA vs. NCBI nr
Match: XP_022997831.1 (actin-depolymerizing factor 2-like [Cucurbita maxima])

HSP 1 Score: 278.5 bits (711), Expect = 1.3e-71
Identity = 138/139 (99.28%), Postives = 139/139 (100.00%), Query Frame = 0


Query: 1   MANAASGMAVHDECKLKFLELKTKRTYRYIVYKIEEKQKQVIVEKVGEPSQSYEDFTACL 60
           MANAASGMAVHDECKLKFLELKTKRTYRYIVYKIEEKQKQVIVEKVGEPSQSYEDFTACL
Sbjct: 1   MANAASGMAVHDECKLKFLELKTKRTYRYIVYKIEEKQKQVIVEKVGEPSQSYEDFTACL 60

Query: 61  PADECRYAVYDFDFLTKENVQKSRIFFIAWSPDISKVRSKMIYASSKDKFRRELDGIQIE 120
           PADECRYAVYDFDFLTKENVQKSRIFFIAWSPD+SKVRSKMIYASSKDKFRRELDGIQIE
Sbjct: 61  PADECRYAVYDFDFLTKENVQKSRIFFIAWSPDVSKVRSKMIYASSKDKFRRELDGIQIE 120

Query: 121 LQATDPTEMGLDVFNSRAK 140
           LQATDPTEMGLDVFNSRAK
Sbjct: 121 LQATDPTEMGLDVFNSRAK 139

[Close]

BLAST of Carg12331-RA vs. NCBI nr
Match: XP_022156171.1 (actin-depolymerizing factor 2 [Momordica charantia])

HSP 1 Score: 275.0 bits (702), Expect = 1.4e-70
Identity = 136/139 (97.84%), Postives = 138/139 (99.28%), Query Frame = 0


Query: 1   MANAASGMAVHDECKLKFLELKTKRTYRYIVYKIEEKQKQVIVEKVGEPSQSYEDFTACL 60
           MANAASGMAVHD+CKLKFLELKTKRTYRYIVYKIEEKQKQVIVEK+GEP QSYEDFTACL
Sbjct: 1   MANAASGMAVHDDCKLKFLELKTKRTYRYIVYKIEEKQKQVIVEKLGEPGQSYEDFTACL 60

Query: 61  PADECRYAVYDFDFLTKENVQKSRIFFIAWSPDISKVRSKMIYASSKDKFRRELDGIQIE 120
           PADECRYAVYDFDFLTKENVQKSRIFFIAWSPDISKVRSKMIYASSKDKFRRELDGIQIE
Sbjct: 61  PADECRYAVYDFDFLTKENVQKSRIFFIAWSPDISKVRSKMIYASSKDKFRRELDGIQIE 120

Query: 121 LQATDPTEMGLDVFNSRAK 140
           LQATDPTEMGLDVFNSRAK
Sbjct: 121 LQATDPTEMGLDVFNSRAK 139

[Close]

BLAST of Carg12331-RA vs. NCBI nr
Match: XP_022964654.1 (actin-depolymerizing factor 2-like [Cucurbita moschata] >XP_022970372.1 actin-depolymerizing factor 2-like [Cucurbita maxima] >XP_023519424.1 actin-depolymerizing factor 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 267.7 bits (683), Expect = 2.2e-68
Identity = 132/139 (94.96%), Postives = 136/139 (97.84%), Query Frame = 0


Query: 1   MANAASGMAVHDECKLKFLELKTKRTYRYIVYKIEEKQKQVIVEKVGEPSQSYEDFTACL 60
           MANAASGMAVHD+CKLKFLELKTKRTYR+IVYKIEEKQKQVIVEKVGEP QSYEDFTACL
Sbjct: 1   MANAASGMAVHDDCKLKFLELKTKRTYRFIVYKIEEKQKQVIVEKVGEPGQSYEDFTACL 60

Query: 61  PADECRYAVYDFDFLTKENVQKSRIFFIAWSPDISKVRSKMIYASSKDKFRRELDGIQIE 120
           PADECRYAVYDFDFLTKENVQKSRIFFIAWSPD+SKVRSKMIYASSKDKFRRELDGIQIE
Sbjct: 61  PADECRYAVYDFDFLTKENVQKSRIFFIAWSPDVSKVRSKMIYASSKDKFRRELDGIQIE 120

Query: 121 LQATDPTEMGLDVFNSRAK 140
           LQATDP+EM LDVF SRAK
Sbjct: 121 LQATDPSEMDLDVFKSRAK 139

[Close]

BLAST of Carg12331-RA vs. NCBI nr
Match: XP_004149045.1 (PREDICTED: actin-depolymerizing factor 2 [Cucumis sativus] >KGN65663.1 hypothetical protein Csa_1G480180 [Cucumis sativus])

HSP 1 Score: 256.5 bits (654), Expect = 5.1e-65
Identity = 127/139 (91.37%), Postives = 132/139 (94.96%), Query Frame = 0


Query: 1   MANAASGMAVHDECKLKFLELKTKRTYRYIVYKIEEKQKQVIVEKVGEPSQSYEDFTACL 60
           MANAASGMAVHD+CKLKFLELKTKRTYRYIVYKIEEKQKQV VEK+GEP QSYEDFTACL
Sbjct: 1   MANAASGMAVHDDCKLKFLELKTKRTYRYIVYKIEEKQKQVTVEKLGEPGQSYEDFTACL 60

Query: 61  PADECRYAVYDFDFLTKENVQKSRIFFIAWSPDISKVRSKMIYASSKDKFRRELDGIQIE 120
           PADECRYAVYDF+FLT+ NV KSRIFFIAWSPD SKVRSKMIYASSKDKFRRELDGIQIE
Sbjct: 61  PADECRYAVYDFEFLTEGNVPKSRIFFIAWSPDTSKVRSKMIYASSKDKFRRELDGIQIE 120

Query: 121 LQATDPTEMGLDVFNSRAK 140
           LQATDP+EM LDVF SRAK
Sbjct: 121 LQATDPSEMDLDVFKSRAK 139

[Close]

BLAST of Carg12331-RA vs. TAIR10
Match: AT3G46010.2 (actin depolymerizing factor 1)

HSP 1 Score: 241.1 bits (614), Expect = 4.0e-64
Identity = 114/137 (83.21%), Postives = 129/137 (94.16%), Query Frame = 0


Query: 2   ANAASGMAVHDECKLKFLELKTKRTYRYIVYKIEEKQKQVIVEKVGEPSQSYEDFTACLP 61
           ANAASGMAVHD+CKL+FLELK KRT+R+IVYKIEEKQKQV+VEKVG+P Q+YE+F ACLP
Sbjct: 13  ANAASGMAVHDDCKLRFLELKAKRTHRFIVYKIEEKQKQVVVEKVGQPIQTYEEFAACLP 72

Query: 62  ADECRYAVYDFDFLTKENVQKSRIFFIAWSPDISKVRSKMIYASSKDKFRRELDGIQIEL 121
           ADECRYA+YDFDF+T EN QKS+IFFIAW PDI+KVRSKMIYASSKD+F+RELDGIQ+EL
Sbjct: 73  ADECRYAIYDFDFVTAENCQKSKIFFIAWCPDIAKVRSKMIYASSKDRFKRELDGIQVEL 132

Query: 122 QATDPTEMGLDVFNSRA 139
           QATDPTEM LDVF SRA
Sbjct: 133 QATDPTEMDLDVFRSRA 149

[Close]

BLAST of Carg12331-RA vs. TAIR10
Match: AT5G59890.1 (actin depolymerizing factor 4)

HSP 1 Score: 235.0 bits (598), Expect = 2.9e-62
Identity = 113/137 (82.48%), Postives = 126/137 (91.97%), Query Frame = 0


Query: 1   MANAASGMAVHDECKLKFLELKTKRTYRYIVYKIEEKQKQVIVEKVGEPSQSYEDFTACL 60
           MANAASGMAVHD+CKL+FLELK KRT+R+IVYKIEEKQKQVIVEKVGEP  +YEDF A L
Sbjct: 1   MANAASGMAVHDDCKLRFLELKAKRTHRFIVYKIEEKQKQVIVEKVGEPILTYEDFAASL 60

Query: 61  PADECRYAVYDFDFLTKENVQKSRIFFIAWSPDISKVRSKMIYASSKDKFRRELDGIQIE 120
           PADECRYA+YDFDF+T EN QKS+IFFIAW PD++KVRSKMIYASSKD+F+RELDGIQ+E
Sbjct: 61  PADECRYAIYDFDFVTAENCQKSKIFFIAWCPDVAKVRSKMIYASSKDRFKRELDGIQVE 120

Query: 121 LQATDPTEMGLDVFNSR 138
           LQATDPTEM LDV  SR
Sbjct: 121 LQATDPTEMDLDVLKSR 137

[Close]

BLAST of Carg12331-RA vs. TAIR10
Match: AT5G59880.1 (actin depolymerizing factor 3)

HSP 1 Score: 230.3 bits (586), Expect = 7.1e-61
Identity = 108/138 (78.26%), Postives = 127/138 (92.03%), Query Frame = 0


Query: 1   MANAASGMAVHDECKLKFLELKTKRTYRYIVYKIEEKQKQVIVEKVGEPSQSYEDFTACL 60
           MANAASGMAVHD+CKLKF+ELKTKRT+R+I+YKIEE QKQVIVEK+GEP Q++ED  A L
Sbjct: 1   MANAASGMAVHDDCKLKFMELKTKRTHRFIIYKIEELQKQVIVEKIGEPGQTHEDLAASL 60

Query: 61  PADECRYAVYDFDFLTKENVQKSRIFFIAWSPDISKVRSKMIYASSKDKFRRELDGIQIE 120
           PADECRYA++DFDF++ E V +SRIFF+AWSPD ++VRSKMIYASSKD+F+RELDGIQ+E
Sbjct: 61  PADECRYAIFDFDFVSSEGVPRSRIFFVAWSPDTARVRSKMIYASSKDRFKRELDGIQVE 120

Query: 121 LQATDPTEMGLDVFNSRA 139
           LQATDPTEM LDVF SRA
Sbjct: 121 LQATDPTEMDLDVFKSRA 138

[Close]

BLAST of Carg12331-RA vs. TAIR10
Match: AT3G46000.1 (actin depolymerizing factor 2)

HSP 1 Score: 227.6 bits (579), Expect = 4.6e-60
Identity = 110/137 (80.29%), Postives = 124/137 (90.51%), Query Frame = 0


Query: 1   MANAASGMAVHDECKLKFLELKTKRTYRYIVYKIEEKQKQVIVEKVGEPSQSYEDFTACL 60
           MANAASGMAVHD+CKLKF+ELK KRT+R IVYKIE+  KQVIVEK+GEP QSY+DF A L
Sbjct: 1   MANAASGMAVHDDCKLKFMELKAKRTFRTIVYKIED--KQVIVEKLGEPEQSYDDFAASL 60

Query: 61  PADECRYAVYDFDFLTKENVQKSRIFFIAWSPDISKVRSKMIYASSKDKFRRELDGIQIE 120
           PAD+CRY +YDFDF+T EN QKS+IFFIAWSPD +KVR KMIYASSKD+F+RELDGIQ+E
Sbjct: 61  PADDCRYCIYDFDFVTAENCQKSKIFFIAWSPDTAKVRDKMIYASSKDRFKRELDGIQVE 120

Query: 121 LQATDPTEMGLDVFNSR 138
           LQATDPTEMGLDVF SR
Sbjct: 121 LQATDPTEMGLDVFKSR 135

[Close]

BLAST of Carg12331-RA vs. TAIR10
Match: AT1G01750.1 (actin depolymerizing factor 11)

HSP 1 Score: 212.6 bits (540), Expect = 1.5e-55
Identity = 98/137 (71.53%), Postives = 119/137 (86.86%), Query Frame = 0


Query: 1   MANAASGMAVHDECKLKFLELKTKRTYRYIVYKIEEKQKQVIVEKVGEPSQSYEDFTACL 60
           MAN+ASGM V DECKLKFLELK KR YR+IV+KI+EK +QV+++K+G P ++YEDFT  +
Sbjct: 1   MANSASGMHVSDECKLKFLELKAKRNYRFIVFKIDEKAQQVMIDKLGNPEETYEDFTRSI 60

Query: 61  PADECRYAVYDFDFLTKENVQKSRIFFIAWSPDISKVRSKMIYASSKDKFRRELDGIQIE 120
           P DECRYAVYD+DF T EN QKS+IFFIAWSPD S+VRSKM+YASSKD+F+RELDGIQ+E
Sbjct: 61  PEDECRYAVYDYDFTTPENCQKSKIFFIAWSPDTSRVRSKMLYASSKDRFKRELDGIQVE 120

Query: 121 LQATDPTEMGLDVFNSR 138
           LQATDP+EM LD+   R
Sbjct: 121 LQATDPSEMSLDIIKGR 137

[Close]

BLAST of Carg12331-RA vs. Swiss-Prot
Match: sp|Q9FVI1|ADF2_PETHY (Actin-depolymerizing factor 2 OS=Petunia hybrida OX=4102 GN=ADF2 PE=2 SV=1)

HSP 1 Score: 249.2 bits (635), Expect = 2.7e-65
Identity = 119/138 (86.23%), Postives = 133/138 (96.38%), Query Frame = 0


Query: 1   MANAASGMAVHDECKLKFLELKTKRTYRYIVYKIEEKQKQVIVEKVGEPSQSYEDFTACL 60
           MANAASGMAVHD+CKLKFLELK KRTYR+I+YKIEEKQK+V+VEK+GEP++SYEDFTA L
Sbjct: 1   MANAASGMAVHDDCKLKFLELKAKRTYRFIIYKIEEKQKEVVVEKLGEPTESYEDFTAGL 60

Query: 61  PADECRYAVYDFDFLTKENVQKSRIFFIAWSPDISKVRSKMIYASSKDKFRRELDGIQIE 120
           PADECRYAVYDFDF+TKEN QKSRIFFIAWSPD ++VRSKMIYASSKD+F+RELDGIQ+E
Sbjct: 61  PADECRYAVYDFDFMTKENHQKSRIFFIAWSPDTARVRSKMIYASSKDRFKRELDGIQVE 120

Query: 121 LQATDPTEMGLDVFNSRA 139
           LQATDPTEMGLDVF SRA
Sbjct: 121 LQATDPTEMGLDVFRSRA 138

[Close]

BLAST of Carg12331-RA vs. Swiss-Prot
Match: sp|Q39250|ADF1_ARATH (Actin-depolymerizing factor 1 OS=Arabidopsis thaliana OX=3702 GN=ADF1 PE=1 SV=1)

HSP 1 Score: 242.7 bits (618), Expect = 2.5e-63
Identity = 115/138 (83.33%), Postives = 130/138 (94.20%), Query Frame = 0


Query: 1   MANAASGMAVHDECKLKFLELKTKRTYRYIVYKIEEKQKQVIVEKVGEPSQSYEDFTACL 60
           MANAASGMAVHD+CKL+FLELK KRT+R+IVYKIEEKQKQV+VEKVG+P Q+YE+F ACL
Sbjct: 1   MANAASGMAVHDDCKLRFLELKAKRTHRFIVYKIEEKQKQVVVEKVGQPIQTYEEFAACL 60

Query: 61  PADECRYAVYDFDFLTKENVQKSRIFFIAWSPDISKVRSKMIYASSKDKFRRELDGIQIE 120
           PADECRYA+YDFDF+T EN QKS+IFFIAW PDI+KVRSKMIYASSKD+F+RELDGIQ+E
Sbjct: 61  PADECRYAIYDFDFVTAENCQKSKIFFIAWCPDIAKVRSKMIYASSKDRFKRELDGIQVE 120

Query: 121 LQATDPTEMGLDVFNSRA 139
           LQATDPTEM LDVF SRA
Sbjct: 121 LQATDPTEMDLDVFRSRA 138

[Close]

BLAST of Carg12331-RA vs. Swiss-Prot
Match: sp|Q9FVI2|ADF1_PETHY (Actin-depolymerizing factor 1 OS=Petunia hybrida OX=4102 GN=ADF1 PE=2 SV=1)

HSP 1 Score: 235.0 bits (598), Expect = 5.2e-61
Identity = 112/138 (81.16%), Postives = 127/138 (92.03%), Query Frame = 0


Query: 1   MANAASGMAVHDECKLKFLELKTKRTYRYIVYKIEEKQKQVIVEKVGEPSQSYEDFTACL 60
           MANAASGMAVHD+CKL+FLELK KRT+R+IVYKIEEKQKQV+VEK+GEP++SYEDF A L
Sbjct: 1   MANAASGMAVHDDCKLRFLELKAKRTHRFIVYKIEEKQKQVVVEKIGEPTESYEDFAASL 60

Query: 61  PADECRYAVYDFDFLTKENVQKSRIFFIAWSPDISKVRSKMIYASSKDKFRRELDGIQIE 120
           P +ECRYAVYDFDF+T EN QKSRIFFIAW PD ++VRSKMIYASSKD+F+RELDGIQ+E
Sbjct: 61  PENECRYAVYDFDFVTAENCQKSRIFFIAWCPDTARVRSKMIYASSKDRFKRELDGIQVE 120

Query: 121 LQATDPTEMGLDVFNSRA 139
           LQA DPTEMGLDV  SRA
Sbjct: 121 LQACDPTEMGLDVIQSRA 138

[Close]

BLAST of Carg12331-RA vs. Swiss-Prot
Match: sp|Q9ZSK3|ADF4_ARATH (Actin-depolymerizing factor 4 OS=Arabidopsis thaliana OX=3702 GN=ADF4 PE=1 SV=2)

HSP 1 Score: 235.0 bits (598), Expect = 5.2e-61
Identity = 113/137 (82.48%), Postives = 126/137 (91.97%), Query Frame = 0


Query: 1   MANAASGMAVHDECKLKFLELKTKRTYRYIVYKIEEKQKQVIVEKVGEPSQSYEDFTACL 60
           MANAASGMAVHD+CKL+FLELK KRT+R+IVYKIEEKQKQVIVEKVGEP  +YEDF A L
Sbjct: 1   MANAASGMAVHDDCKLRFLELKAKRTHRFIVYKIEEKQKQVIVEKVGEPILTYEDFAASL 60

Query: 61  PADECRYAVYDFDFLTKENVQKSRIFFIAWSPDISKVRSKMIYASSKDKFRRELDGIQIE 120
           PADECRYA+YDFDF+T EN QKS+IFFIAW PD++KVRSKMIYASSKD+F+RELDGIQ+E
Sbjct: 61  PADECRYAIYDFDFVTAENCQKSKIFFIAWCPDVAKVRSKMIYASSKDRFKRELDGIQVE 120

Query: 121 LQATDPTEMGLDVFNSR 138
           LQATDPTEM LDV  SR
Sbjct: 121 LQATDPTEMDLDVLKSR 137

[Close]

BLAST of Carg12331-RA vs. Swiss-Prot
Match: sp|Q9ZSK4|ADF3_ARATH (Actin-depolymerizing factor 3 OS=Arabidopsis thaliana OX=3702 GN=ADF3 PE=1 SV=1)

HSP 1 Score: 230.3 bits (586), Expect = 1.3e-59
Identity = 108/138 (78.26%), Postives = 127/138 (92.03%), Query Frame = 0


Query: 1   MANAASGMAVHDECKLKFLELKTKRTYRYIVYKIEEKQKQVIVEKVGEPSQSYEDFTACL 60
           MANAASGMAVHD+CKLKF+ELKTKRT+R+I+YKIEE QKQVIVEK+GEP Q++ED  A L
Sbjct: 1   MANAASGMAVHDDCKLKFMELKTKRTHRFIIYKIEELQKQVIVEKIGEPGQTHEDLAASL 60

Query: 61  PADECRYAVYDFDFLTKENVQKSRIFFIAWSPDISKVRSKMIYASSKDKFRRELDGIQIE 120
           PADECRYA++DFDF++ E V +SRIFF+AWSPD ++VRSKMIYASSKD+F+RELDGIQ+E
Sbjct: 61  PADECRYAIFDFDFVSSEGVPRSRIFFVAWSPDTARVRSKMIYASSKDRFKRELDGIQVE 120

Query: 121 LQATDPTEMGLDVFNSRA 139
           LQATDPTEM LDVF SRA
Sbjct: 121 LQATDPTEMDLDVFKSRA 138

[Close]

BLAST of Carg12331-RA vs. TrEMBL
Match: tr|A0A0A0LV73|A0A0A0LV73_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G480180 PE=3 SV=1)

HSP 1 Score: 256.5 bits (654), Expect = 3.4e-65
Identity = 127/139 (91.37%), Postives = 132/139 (94.96%), Query Frame = 0


Query: 1   MANAASGMAVHDECKLKFLELKTKRTYRYIVYKIEEKQKQVIVEKVGEPSQSYEDFTACL 60
           MANAASGMAVHD+CKLKFLELKTKRTYRYIVYKIEEKQKQV VEK+GEP QSYEDFTACL
Sbjct: 1   MANAASGMAVHDDCKLKFLELKTKRTYRYIVYKIEEKQKQVTVEKLGEPGQSYEDFTACL 60

Query: 61  PADECRYAVYDFDFLTKENVQKSRIFFIAWSPDISKVRSKMIYASSKDKFRRELDGIQIE 120
           PADECRYAVYDF+FLT+ NV KSRIFFIAWSPD SKVRSKMIYASSKDKFRRELDGIQIE
Sbjct: 61  PADECRYAVYDFEFLTEGNVPKSRIFFIAWSPDTSKVRSKMIYASSKDKFRRELDGIQIE 120

Query: 121 LQATDPTEMGLDVFNSRAK 140
           LQATDP+EM LDVF SRAK
Sbjct: 121 LQATDPSEMDLDVFKSRAK 139

[Close]

BLAST of Carg12331-RA vs. TrEMBL
Match: tr|A0A1S3C732|A0A1S3C732_CUCME (actin-depolymerizing factor 2 OS=Cucumis melo OX=3656 GN=LOC103497215 PE=3 SV=1)

HSP 1 Score: 256.1 bits (653), Expect = 4.4e-65
Identity = 127/139 (91.37%), Postives = 132/139 (94.96%), Query Frame = 0


Query: 1   MANAASGMAVHDECKLKFLELKTKRTYRYIVYKIEEKQKQVIVEKVGEPSQSYEDFTACL 60
           MANAASGMAVHD+CKLKFLELKTKRTYR+IVYKIEEKQKQV VEKVGEP QSYEDFTACL
Sbjct: 1   MANAASGMAVHDDCKLKFLELKTKRTYRFIVYKIEEKQKQVTVEKVGEPGQSYEDFTACL 60

Query: 61  PADECRYAVYDFDFLTKENVQKSRIFFIAWSPDISKVRSKMIYASSKDKFRRELDGIQIE 120
           PADECRYAVYDF+FLT+ NV KSRIFFIAWSPD SKVRSKMIYASSKDKFRRELDGIQIE
Sbjct: 61  PADECRYAVYDFEFLTEGNVPKSRIFFIAWSPDTSKVRSKMIYASSKDKFRRELDGIQIE 120

Query: 121 LQATDPTEMGLDVFNSRAK 140
           LQATDP+EM LDVF SRAK
Sbjct: 121 LQATDPSEMDLDVFKSRAK 139

[Close]

BLAST of Carg12331-RA vs. TrEMBL
Match: tr|E5GCP0|E5GCP0_CUCME (Actin depolymerizing factor-like protein OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1)

HSP 1 Score: 256.1 bits (653), Expect = 4.4e-65
Identity = 127/139 (91.37%), Postives = 132/139 (94.96%), Query Frame = 0


Query: 1   MANAASGMAVHDECKLKFLELKTKRTYRYIVYKIEEKQKQVIVEKVGEPSQSYEDFTACL 60
           MANAASGMAVHD+CKLKFLELKTKRTYR+IVYKIEEKQKQV VEKVGEP QSYEDFTACL
Sbjct: 1   MANAASGMAVHDDCKLKFLELKTKRTYRFIVYKIEEKQKQVTVEKVGEPGQSYEDFTACL 60

Query: 61  PADECRYAVYDFDFLTKENVQKSRIFFIAWSPDISKVRSKMIYASSKDKFRRELDGIQIE 120
           PADECRYAVYDF+FLT+ NV KSRIFFIAWSPD SKVRSKMIYASSKDKFRRELDGIQIE
Sbjct: 61  PADECRYAVYDFEFLTEGNVPKSRIFFIAWSPDTSKVRSKMIYASSKDKFRRELDGIQIE 120

Query: 121 LQATDPTEMGLDVFNSRAK 140
           LQATDP+EM LDVF SRAK
Sbjct: 121 LQATDPSEMDLDVFKSRAK 139

[Close]

BLAST of Carg12331-RA vs. TrEMBL
Match: tr|A0A2I0I5Y4|A0A2I0I5Y4_PUNGR (Uncharacterized protein OS=Punica granatum OX=22663 GN=CRG98_040264 PE=3 SV=1)

HSP 1 Score: 253.4 bits (646), Expect = 2.9e-64
Identity = 124/138 (89.86%), Postives = 132/138 (95.65%), Query Frame = 0


Query: 1   MANAASGMAVHDECKLKFLELKTKRTYRYIVYKIEEKQKQVIVEKVGEPSQSYEDFTACL 60
           MANAASGMAVHD+CKLKFLELK KRTYRYIV+KIEEKQKQVIVEKVGEPSQSYEDF A L
Sbjct: 1   MANAASGMAVHDDCKLKFLELKAKRTYRYIVFKIEEKQKQVIVEKVGEPSQSYEDFAASL 60

Query: 61  PADECRYAVYDFDFLTKENVQKSRIFFIAWSPDISKVRSKMIYASSKDKFRRELDGIQIE 120
           PADECRYAVYDFDF+T+EN QKSRIFFIAWSPD S+VRSKMIYASSKD+F+RELDGIQ+E
Sbjct: 61  PADECRYAVYDFDFVTEENCQKSRIFFIAWSPDTSRVRSKMIYASSKDRFKRELDGIQVE 120

Query: 121 LQATDPTEMGLDVFNSRA 139
           LQATDPTEMGLDVF SRA
Sbjct: 121 LQATDPTEMGLDVFRSRA 138

[Close]

BLAST of Carg12331-RA vs. TrEMBL
Match: tr|A9P876|A9P876_POPTR (Actin-depolymerizing factor family protein OS=Populus trichocarpa OX=3694 GN=POPTR_001G236500v3 PE=2 SV=1)

HSP 1 Score: 252.7 bits (644), Expect = 4.9e-64
Identity = 123/138 (89.13%), Postives = 133/138 (96.38%), Query Frame = 0


Query: 1   MANAASGMAVHDECKLKFLELKTKRTYRYIVYKIEEKQKQVIVEKVGEPSQSYEDFTACL 60
           MANAASGMAVHD+CKLKFLELK KRTYR+IVYKIEEKQKQVIVEK+GEP+QSYEDFTA L
Sbjct: 1   MANAASGMAVHDDCKLKFLELKAKRTYRFIVYKIEEKQKQVIVEKLGEPAQSYEDFTASL 60

Query: 61  PADECRYAVYDFDFLTKENVQKSRIFFIAWSPDISKVRSKMIYASSKDKFRRELDGIQIE 120
           PADECRYAVYDFDF+T+ENVQKSRIFFIAWSPD S+VRSKMIYASSKD+F+RELDGIQ+E
Sbjct: 61  PADECRYAVYDFDFVTEENVQKSRIFFIAWSPDTSRVRSKMIYASSKDRFKRELDGIQVE 120

Query: 121 LQATDPTEMGLDVFNSRA 139
           LQATDPTEMGLDV  SRA
Sbjct: 121 LQATDPTEMGLDVIKSRA 138

The following BLAST results are available for this feature:

BLAST of Carg12331-RA vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022948707.1	9.6e-72	100.00	actin-depolymerizing factor 2-like [Cucurbita moschata] >XP_023521573.1 actin-de...	[more]
XP_022997831.1	1.3e-71	99.28	actin-depolymerizing factor 2-like [Cucurbita maxima]	[more]
XP_022156171.1	1.4e-70	97.84	actin-depolymerizing factor 2 [Momordica charantia]	[more]
XP_022964654.1	2.2e-68	94.96	actin-depolymerizing factor 2-like [Cucurbita moschata] >XP_022970372.1 actin-de...	[more]
XP_004149045.1	5.1e-65	91.37	PREDICTED: actin-depolymerizing factor 2 [Cucumis sativus] >KGN65663.1 hypotheti...	[more]

BLAST of Carg12331-RA vs. TAIR10


Match Name	E-value	Identity	Description
AT3G46010.2	4.0e-64	83.21	actin depolymerizing factor 1	[more]
AT5G59890.1	2.9e-62	82.48	actin depolymerizing factor 4	[more]
AT5G59880.1	7.1e-61	78.26	actin depolymerizing factor 3	[more]
AT3G46000.1	4.6e-60	80.29	actin depolymerizing factor 2	[more]
AT1G01750.1	1.5e-55	71.53	actin depolymerizing factor 11	[more]

BLAST of Carg12331-RA vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q9FVI1\|ADF2_PETHY	2.7e-65	86.23	Actin-depolymerizing factor 2 OS=Petunia hybrida OX=4102 GN=ADF2 PE=2 SV=1	[more]
sp\|Q39250\|ADF1_ARATH	2.5e-63	83.33	Actin-depolymerizing factor 1 OS=Arabidopsis thaliana OX=3702 GN=ADF1 PE=1 SV=1	[more]
sp\|Q9FVI2\|ADF1_PETHY	5.2e-61	81.16	Actin-depolymerizing factor 1 OS=Petunia hybrida OX=4102 GN=ADF1 PE=2 SV=1	[more]
sp\|Q9ZSK3\|ADF4_ARATH	5.2e-61	82.48	Actin-depolymerizing factor 4 OS=Arabidopsis thaliana OX=3702 GN=ADF4 PE=1 SV=2	[more]
sp\|Q9ZSK4\|ADF3_ARATH	1.3e-59	78.26	Actin-depolymerizing factor 3 OS=Arabidopsis thaliana OX=3702 GN=ADF3 PE=1 SV=1	[more]

BLAST of Carg12331-RA vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A0A0LV73\|A0A0A0LV73_CUCSA	3.4e-65	91.37	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G480180 PE=3 SV=1	[more]
tr\|A0A1S3C732\|A0A1S3C732_CUCME	4.4e-65	91.37	actin-depolymerizing factor 2 OS=Cucumis melo OX=3656 GN=LOC103497215 PE=3 SV=1	[more]
tr\|E5GCP0\|E5GCP0_CUCME	4.4e-65	91.37	Actin depolymerizing factor-like protein OS=Cucumis melo subsp. melo OX=412675 P...	[more]
tr\|A0A2I0I5Y4\|A0A2I0I5Y4_PUNGR	2.9e-64	89.86	Uncharacterized protein OS=Punica granatum OX=22663 GN=CRG98_040264 PE=3 SV=1	[more]
tr\|A9P876\|A9P876_POPTR	4.9e-64	89.13	Actin-depolymerizing factor family protein OS=Populus trichocarpa OX=3694 GN=POP...	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **Biological Process**
Term	Definition
GO:0030042	actin filament depolymerization

Vocabulary: **Cellular Component**
Term	Definition
GO:0015629	actin cytoskeleton
GO:0005622	intracellular

Vocabulary: **Molecular Function**
Term	Definition
GO:0003779	actin binding

Vocabulary: **INTERPRO**
Term	Definition
IPR017904	ADF/Cofilin
IPR029006	ADF-H/Gelsolin-like_dom_sf
IPR002108	ADF-H

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030042	actin filament depolymerization
cellular_component	GO:0015629	actin cytoskeleton
cellular_component	GO:0005622	intracellular
molecular_function	GO:0003779	actin binding

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Carg12331	Carg12331	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Carg12331-RA	Carg12331-RA-protein	polypeptide

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg12331-RA.exon.1	Carg12331-RA.exon.1	exon
Carg12331-RA.exon.2	Carg12331-RA.exon.2	exon
Carg12331-RA.exon.3	Carg12331-RA.exon.3	exon

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg12331-RA.five_prime_UTR.1	Carg12331-RA.five_prime_UTR.1	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg12331-RA.CDS.1	Carg12331-RA.CDS.1	CDS
Carg12331-RA.CDS.2	Carg12331-RA.CDS.2	CDS
Carg12331-RA.CDS.3	Carg12331-RA.CDS.3	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg12331-RA.three_prime_UTR.1	Carg12331-RA.three_prime_UTR.1	three_prime_UTR

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002108	Actin-depolymerising factor homology domain	SMART	SM00102	adf_2	coord: 12..139 e-value: 1.1E-55 score: 201.0
IPR002108	Actin-depolymerising factor homology domain	PFAM	PF00241	Cofilin_ADF	coord: 14..135 e-value: 5.9E-36 score: 123.3
IPR002108	Actin-depolymerising factor homology domain	PROSITE	PS51263	ADF_H	coord: 5..139 score: 36.715
IPR029006	ADF-H/Gelsolin-like domain superfamily	GENE3D	G3DSA:3.40.20.10		coord: 1..139 e-value: 9.2E-51 score: 172.9
None	No IPR available	PANTHER	PTHR11913:SF50	SUBFAMILY NOT NAMED	coord: 1..138
None	No IPR available	SUPERFAMILY	SSF55753	Actin depolymerizing proteins	coord: 2..137
IPR017904	ADF/Cofilin	PANTHER	PTHR11913	COFILIN-RELATED	coord: 1..138
IPR017904	ADF/Cofilin	CDD	cd11286	ADF_cofilin_like	coord: 6..138 e-value: 1.70631E-62 score: 189.305