Carg12303-RA (mRNA) Silver-seed gourd

NameCarg12303-RA
TypemRNA
OrganismCucurbita argyrosperma (Silver-seed gourd)
Descriptioninteractor of constitutive active ROPs 3-like
LocationCucurbita_argyrosperma_scaffold_074 : 189125 .. 191567 (-)
Sequence length2029
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexonthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCAAATCATAGGCAATCAAACAAGTTCTTTCCTCAACAGGAATTCCTTCTCAGAACCACCTCAAAAGGTTTCTCCACGAGTTGCTCGGAAACTTCGTCCCGCTTCTCTGGAATGTGATTCTGCTTCCTCAACCCGAACAAATAATCGAACCATTAAAGAACGAAGCCCGAAAGTCGTCGATCGAAAGTCGCCTAGAAGCCCTGCAAGTGAGGTAATATTACTTGTACCTCTTGGTTCTTGTTCAAAGTTGAGCTTTGGAATGGTGCTTGCACCTCCATTCATCTCGATAGAAGTTGGATTCTTATAATAATCGCTCCCACGTCAGTTGGAGAGGGAAACGAAACATTGTTTATAAGGGTGTGAACATCTCTTCTAGCTAACGTGCTTTATAAACCTTGAGGGGAAGCCCGAAAGAGAACAATATCTGCTAGTGGTGGGCTTGAGCTGTTACAAACGATAACAAAGCCAGACATTAGATGGTGTACCAATGAGGTCACTGAGCTCTGAAGGGGCGGTGTGCCAGTAAAGACACTGGGCTCCAAAGAGGGGTGGATTGTGAGATCCCACGTCGGTTAGAGAAGGGAACGAAACATTTTTTGAACTTTATTTCTCGTTGCTACTTCAGAAAAAACGTCCAAGCAAGATTTCCGAGTTGGAATCTCAGGTCTGTCAGCTGCAAGAGAATCTAACCAAAGTGAAGGATCAGTTATGTTTATCTGAATCATGCAAGAAGGAAGTCAAGCAAGAGGCCGAGGAGGCCAAGAATCAACTAGAAGCCATGGCCTTAAAGCTTCAAGAGTCACAAGAACAAGTACTTGAGCTATCTACTTCCAAGGAAGCTCATGTCATAGAACTACAAGAGATATCACTCGAACGAGATCGAGCTTGGCAAGCCGAGCTTGAGACGATTCAAGAACAGCACAAACTCGACTACGCTGCGTTGGCTACTGCAGTGAATGAGATTCATCAGCTGAAGATCCACCTTGAGATGGTAGCTCAATCCGAAACAAAAAAGATCAAGCTTACGGACTCCGAAAATAACGAACTTGATAGCTTGAGAATTCACCTTGCAGAAGCTCTTTGCGTTGTGGAAAGAATGAAAAATGAGCTCGAAAGTTGTCGAGAATCCGAAGCTCAAGCTCGGTTACTTGCTAATGAAACTCTAGCACAACTAGAAGACGCCAAAAAACGAGTCGAGGAGCTTAGATACGACGGTATGGAAGCAATGAAGGGATACCATTCCATTGCTTCAGAGCTGGATGAATCAAAGGCACGGATTAGCGTGTTAGAAGAACTCGTTAGTAAACTCAAAACCGACGTTGCGAATCCAGCAGTCGAAAACGAGAAACAGGGAGATCATATTGATGAACATTGTTGTCTCAAAACAGAGATAGAACAGCTGAAATCCGCCTTGGAAATGGCTGAGACAAAATACCATGAAGAACAAATTCAGAATGCAGTCAAGATAAGAACAGCTTATGAACAAGTTGAGCAGATTAAAACCAACATGAATTCTAAGGAGTCTAATCTAGAGCAGGAGCTGAAAAAATTGAAGGTCGATATCGACGAGTTAAAGGCCGACTTGATGGATAAGGAGACCGAATTGCAGGCTATTTCGGAGGAGAACGAGCAGCTGCACGCGAAAATTCAAAAGAATCTATCGAGCCAGAGAGAATATGAACTCGAAAGAGAGATTAAGGAATTGAAGGATCATCTTGCAGTGATGAAGTCCAATCTCATTGACAAGGATAGACAATTCCGTAGCGTTTTGGACGAGAACGATACGCTAAAACTCGATATAAACAAAAGGATAATGATTCGAGCGAAAGCAATCGATGGGACTACAGAAGTTGAGGACACAACGACTGTCGAACAAGAGGCTCTCACAAGACTTGGCGTTACGATAGAGGAGACAGAAAAAAGTAACAAGAGGGCGGCTCGAGTGTCCGAACAGTTGGAGGCTGCACAGGCTGCAAATGTCGAAATAGAAACGGAGTTGAGACGGTTGAGGGTGCAGTCAGACCAATGGAGGAAGGCTGCAGAGGCTGCAGCCGCAATGCTCTCTGCTGGAAACAATGGCAAGTTCATGGACCGATCGGCGTCGTTGGACAGCAGCTACGACTCGGATTCTGCAGGAAAAGTGAACAGGAGTTGTGCTGAAATAGATGATGATTTGCTGAAAAAGAAACATGGGAATGTGCTAAAAAAGATAGGGGTGCTGTGGAAGAAACAACACAAATGAAAGGGTCAGGGCCAGGGCCATGGCCAGGGCTTGGCATGTCTAACTCTGCTTGTGGGGCAGTTTATTTTGTTTTGTTACTCATGTACTGTTGATCTTGATATCATATGAAGCTCAAGAACAGATCTTGATATCATATGAAGCTCAAGAACAGATCTTGATATCATATGAAGCTCAAGAACACTACCCTGTTCAAATTTAG

mRNA sequence

ATGCAAATCATAGGCAATCAAACAAGTTCTTTCCTCAACAGGAATTCCTTCTCAGAACCACCTCAAAAGGTTTCTCCACGAGTTGCTCGGAAACTTCGTCCCGCTTCTCTGGAATGTGATTCTGCTTCCTCAACCCGAACAAATAATCGAACCATTAAAGAACGAAGCCCGAAAGTCGTCGATCGAAAGTCGCCTAGAAGCCCTGCAAGTGAGAAAAAACGTCCAAGCAAGATTTCCGAGTTGGAATCTCAGGTCTGTCAGCTGCAAGAGAATCTAACCAAAGTGAAGGATCAGTTATGTTTATCTGAATCATGCAAGAAGGAAGTCAAGCAAGAGGCCGAGGAGGCCAAGAATCAACTAGAAGCCATGGCCTTAAAGCTTCAAGAGTCACAAGAACAAGTACTTGAGCTATCTACTTCCAAGGAAGCTCATGTCATAGAACTACAAGAGATATCACTCGAACGAGATCGAGCTTGGCAAGCCGAGCTTGAGACGATTCAAGAACAGCACAAACTCGACTACGCTGCGTTGGCTACTGCAGTGAATGAGATTCATCAGCTGAAGATCCACCTTGAGATGGTAGCTCAATCCGAAACAAAAAAGATCAAGCTTACGGACTCCGAAAATAACGAACTTGATAGCTTGAGAATTCACCTTGCAGAAGCTCTTTGCGTTGTGGAAAGAATGAAAAATGAGCTCGAAAGTTGTCGAGAATCCGAAGCTCAAGCTCGGTTACTTGCTAATGAAACTCTAGCACAACTAGAAGACGCCAAAAAACGAGTCGAGGAGCTTAGATACGACGGTATGGAAGCAATGAAGGGATACCATTCCATTGCTTCAGAGCTGGATGAATCAAAGGCACGGATTAGCGTGTTAGAAGAACTCGTTAGTAAACTCAAAACCGACGTTGCGAATCCAGCAGTCGAAAACGAGAAACAGGGAGATCATATTGATGAACATTGTTGTCTCAAAACAGAGATAGAACAGCTGAAATCCGCCTTGGAAATGGCTGAGACAAAATACCATGAAGAACAAATTCAGAATGCAGTCAAGATAAGAACAGCTTATGAACAAGTTGAGCAGATTAAAACCAACATGAATTCTAAGGAGTCTAATCTAGAGCAGGAGCTGAAAAAATTGAAGGTCGATATCGACGAGTTAAAGGCCGACTTGATGGATAAGGAGACCGAATTGCAGGCTATTTCGGAGGAGAACGAGCAGCTGCACGCGAAAATTCAAAAGAATCTATCGAGCCAGAGAGAATATGAACTCGAAAGAGAGATTAAGGAATTGAAGGATCATCTTGCAGTGATGAAGTCCAATCTCATTGACAAGGATAGACAATTCCGTAGCGTTTTGGACGAGAACGATACGCTAAAACTCGATATAAACAAAAGGATAATGATTCGAGCGAAAGCAATCGATGGGACTACAGAAGTTGAGGACACAACGACTGTCGAACAAGAGGCTCTCACAAGACTTGGCGTTACGATAGAGGAGACAGAAAAAAGTAACAAGAGGGCGGCTCGAGTGTCCGAACAGTTGGAGGCTGCACAGGCTGCAAATGTCGAAATAGAAACGGAGTTGAGACGGTTGAGGGTGCAGTCAGACCAATGGAGGAAGGCTGCAGAGGCTGCAGCCGCAATGCTCTCTGCTGGAAACAATGGCAAGTTCATGGACCGATCGGCGTCGTTGGACAGCAGCTACGACTCGGATTCTGCAGGAAAAGTGAACAGGAGTTGTGCTGAAATAGATGATGATTTGCTGAAAAAGAAACATGGGAATGTGCTAAAAAAGATAGGGGTGCTGTGGAAGAAACAACACAAATGAAAGGGTCAGGGCCAGGGCCATGGCCAGGGCTTGGCATGTCTAACTCTGCTTGTGGGGCAGTTTATTTTGTTTTGTTACTCATGTACTGTTGATCTTGATATCATATGAAGCTCAAGAACAGATCTTGATATCATATGAAGCTCAAGAACAGATCTTGATATCATATGAAGCTCAAGAACACTACCCTGTTCAAATTTAG

Coding sequence (CDS)

ATGCAAATCATAGGCAATCAAACAAGTTCTTTCCTCAACAGGAATTCCTTCTCAGAACCACCTCAAAAGGTTTCTCCACGAGTTGCTCGGAAACTTCGTCCCGCTTCTCTGGAATGTGATTCTGCTTCCTCAACCCGAACAAATAATCGAACCATTAAAGAACGAAGCCCGAAAGTCGTCGATCGAAAGTCGCCTAGAAGCCCTGCAAGTGAGAAAAAACGTCCAAGCAAGATTTCCGAGTTGGAATCTCAGGTCTGTCAGCTGCAAGAGAATCTAACCAAAGTGAAGGATCAGTTATGTTTATCTGAATCATGCAAGAAGGAAGTCAAGCAAGAGGCCGAGGAGGCCAAGAATCAACTAGAAGCCATGGCCTTAAAGCTTCAAGAGTCACAAGAACAAGTACTTGAGCTATCTACTTCCAAGGAAGCTCATGTCATAGAACTACAAGAGATATCACTCGAACGAGATCGAGCTTGGCAAGCCGAGCTTGAGACGATTCAAGAACAGCACAAACTCGACTACGCTGCGTTGGCTACTGCAGTGAATGAGATTCATCAGCTGAAGATCCACCTTGAGATGGTAGCTCAATCCGAAACAAAAAAGATCAAGCTTACGGACTCCGAAAATAACGAACTTGATAGCTTGAGAATTCACCTTGCAGAAGCTCTTTGCGTTGTGGAAAGAATGAAAAATGAGCTCGAAAGTTGTCGAGAATCCGAAGCTCAAGCTCGGTTACTTGCTAATGAAACTCTAGCACAACTAGAAGACGCCAAAAAACGAGTCGAGGAGCTTAGATACGACGGTATGGAAGCAATGAAGGGATACCATTCCATTGCTTCAGAGCTGGATGAATCAAAGGCACGGATTAGCGTGTTAGAAGAACTCGTTAGTAAACTCAAAACCGACGTTGCGAATCCAGCAGTCGAAAACGAGAAACAGGGAGATCATATTGATGAACATTGTTGTCTCAAAACAGAGATAGAACAGCTGAAATCCGCCTTGGAAATGGCTGAGACAAAATACCATGAAGAACAAATTCAGAATGCAGTCAAGATAAGAACAGCTTATGAACAAGTTGAGCAGATTAAAACCAACATGAATTCTAAGGAGTCTAATCTAGAGCAGGAGCTGAAAAAATTGAAGGTCGATATCGACGAGTTAAAGGCCGACTTGATGGATAAGGAGACCGAATTGCAGGCTATTTCGGAGGAGAACGAGCAGCTGCACGCGAAAATTCAAAAGAATCTATCGAGCCAGAGAGAATATGAACTCGAAAGAGAGATTAAGGAATTGAAGGATCATCTTGCAGTGATGAAGTCCAATCTCATTGACAAGGATAGACAATTCCGTAGCGTTTTGGACGAGAACGATACGCTAAAACTCGATATAAACAAAAGGATAATGATTCGAGCGAAAGCAATCGATGGGACTACAGAAGTTGAGGACACAACGACTGTCGAACAAGAGGCTCTCACAAGACTTGGCGTTACGATAGAGGAGACAGAAAAAAGTAACAAGAGGGCGGCTCGAGTGTCCGAACAGTTGGAGGCTGCACAGGCTGCAAATGTCGAAATAGAAACGGAGTTGAGACGGTTGAGGGTGCAGTCAGACCAATGGAGGAAGGCTGCAGAGGCTGCAGCCGCAATGCTCTCTGCTGGAAACAATGGCAAGTTCATGGACCGATCGGCGTCGTTGGACAGCAGCTACGACTCGGATTCTGCAGGAAAAGTGAACAGGAGTTGTGCTGAAATAGATGATGATTTGCTGAAAAAGAAACATGGGAATGTGCTAAAAAAGATAGGGGTGCTGTGGAAGAAACAACACAAATGA

Protein sequence

MQIIGNQTSSFLNRNSFSEPPQKVSPRVARKLRPASLECDSASSTRTNNRTIKERSPKVVDRKSPRSPASEKKRPSKISELESQVCQLQENLTKVKDQLCLSESCKKEVKQEAEEAKNQLEAMALKLQESQEQVLELSTSKEAHVIELQEISLERDRAWQAELETIQEQHKLDYAALATAVNEIHQLKIHLEMVAQSETKKIKLTDSENNELDSLRIHLAEALCVVERMKNELESCRESEAQARLLANETLAQLEDAKKRVEELRYDGMEAMKGYHSIASELDESKARISVLEELVSKLKTDVANPAVENEKQGDHIDEHCCLKTEIEQLKSALEMAETKYHEEQIQNAVKIRTAYEQVEQIKTNMNSKESNLEQELKKLKVDIDELKADLMDKETELQAISEENEQLHAKIQKNLSSQREYELEREIKELKDHLAVMKSNLIDKDRQFRSVLDENDTLKLDINKRIMIRAKAIDGTTEVEDTTTVEQEALTRLGVTIEETEKSNKRAARVSEQLEAAQAANVEIETELRRLRVQSDQWRKAAEAAAAMLSAGNNGKFMDRSASLDSSYDSDSAGKVNRSCAEIDDDLLKKKHGNVLKKIGVLWKKQHK
BLAST of Carg12303-RA vs. NCBI nr
Match: XP_022949428.1 (interactor of constitutive active ROPs 3-like isoform X1 [Cucurbita moschata] >XP_022949429.1 interactor of constitutive active ROPs 3-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 800.8 bits (2067), Expect = 3.2e-228
Identity = 529/597 (88.61%), Postives = 535/597 (89.61%), Query Frame = 0

Query: 13  NRNSFSEPPQKVSPRVARKLRPASLECDSASSTRTNNRTIKERSPKVVDRKSPRSPASEK 72
           +RNSFSEPPQKVSPRVARKLRPASLECDSASSTRTNNR IKERSPKVVDRKSPRSPASEK
Sbjct: 6   SRNSFSEPPQKVSPRVARKLRPASLECDSASSTRTNNRIIKERSPKVVDRKSPRSPASEK 65

Query: 73  KRPSKISELESQVCQLQENLTKVKDQLCLSESCKKEVKQEAEEAKNQLEAMALKLQXXXX 132
           KRPSKISELESQVCQLQENLTKVKDQLCLSESCKKEVKQEAEEAK QLEAMALKL XXXX
Sbjct: 66  KRPSKISELESQVCQLQENLTKVKDQLCLSESCKKEVKQEAEEAKTQLEAMALKLXXXXX 125

Query: 133 XXXXXXXXXXXXXXXXXXXXXERDRAWQXXXXXXXXXHKLDYAALATAVNEIHQLKIHLE 192
           XXXXXXXXXXXXXXXXXX   ERDRAWQ         HKLDYAALA+AVNEIHQLKIHLE
Sbjct: 126 XXXXXXXXXXXXXXXXXXISLERDRAWQAELETIQEQHKLDYAALASAVNEIHQLKIHLE 185

Query: 193 MVAQSETKKIKLTDSENNELDSLRIHLAEALCVXXXXXXXXXXXXXXXXXXXXXXXXXXX 252
           MVAQSETKKI LTDSENNELDSLR HLAEALC XXXXXXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 186 MVAQSETKKINLTDSENNELDSLRTHLAEALCXXXXXXXXXXXXXXXXXXXXXXXXXXXX 245

Query: 253 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKARISVLEELVSKLKTDVANPAVENEK 312
           XXXXXXXXXXXXXXXXXXXX             KARISVLEELVSKLKTDVANPAVENEK
Sbjct: 246 XXXXXXXXXXXXXXXXXXXXKGYHSIASELDESKARISVLEELVSKLKTDVANPAVENEK 305

Query: 313 QGDHIDEHCCLKTEIEQLKSALEMAETKYHEEQIQNAVKIRTAYEQVEQIKTNMNSKXXX 372
           QGDHIDEHCCLK+EIEQLKSALEMAETKYHEEQIQNAVK+RTAYEQVEQIKTNMNS+   
Sbjct: 306 QGDHIDEHCCLKSEIEQLKSALEMAETKYHEEQIQNAVKVRTAYEQVEQIKTNMNSRESN 365

Query: 373 XXXXXXXXXXXXXXXXXXXXXXXXXXXAISEENEQLHAKIQKNLSSQREYELEREIKELK 432
                                      AISEENEQLHAKIQKNLSSQREYELEREIKELK
Sbjct: 366 LEQELKKSKVDIDELKADLMDKETELQAISEENEQLHAKIQKNLSSQREYELEREIKELK 425

Query: 433 DHLAVMKSNLIDKDRQFRSVLDENDTLKLDINKRIMIRAKAIDGTTEVEDTTTVEQEALT 492
           DHLAVMKSNLIDKDRQFRSVLDENDTLKLDINKRIMIRAKA DGTTEVEDTTTVEQEALT
Sbjct: 426 DHLAVMKSNLIDKDRQFRSVLDENDTLKLDINKRIMIRAKASDGTTEVEDTTTVEQEALT 485

Query: 493 RLGVTIEETEKSNKRAARVSEQLEAAQAANVEIETELRRLRVQSDQWRKAAEAAAAMLSA 552
           RLGVT+EETEKSNKRAARVSEQLEAAQAANVEIETELRRLRVQSDQWRKAAEAAAAMLSA
Sbjct: 486 RLGVTMEETEKSNKRAARVSEQLEAAQAANVEIETELRRLRVQSDQWRKAAEAAAAMLSA 545

Query: 553 GNNGKFMDRSASLDSSYDSDSAGKVNRSCAEIDDDLLKKKHGNVLKKIGVLWKKQHK 610
           GNNGKFMDRSASLDSSYDSDSAGKVNRSCAEIDDDLLKKKHGNVLKKIGVLWKKQHK
Sbjct: 546 GNNGKFMDRSASLDSSYDSDSAGKVNRSCAEIDDDLLKKKHGNVLKKIGVLWKKQHK 602

BLAST of Carg12303-RA vs. NCBI nr
Match: XP_023523260.1 (interactor of constitutive active ROPs 3-like [Cucurbita pepo subsp. pepo] >XP_023523261.1 interactor of constitutive active ROPs 3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 765.8 bits (1976), Expect = 1.1e-217
Identity = 530/597 (88.78%), Postives = 537/597 (89.95%), Query Frame = 0

Query: 13  NRNSFSEPPQKVSPRVARKLRPASLECDSASSTRTNNRTIKERSPKVVDRKSPRSPASEK 72
           +RNSFSEPPQKVSPRVARKLRPASLECDSASS+RTNNRTIKERSPKVVDRKSPRSPASEK
Sbjct: 6   SRNSFSEPPQKVSPRVARKLRPASLECDSASSSRTNNRTIKERSPKVVDRKSPRSPASEK 65

Query: 73  KRPSKISELESQVCQLQENLTKVKDQLCLSESCKKEVKQEAEEAKNQLEAMALKLQXXXX 132
           KRPSKISELESQVCQLQENLTKVKDQLCLSESCKKEVKQEAEEAKNQLEAMALKLQXXXX
Sbjct: 66  KRPSKISELESQVCQLQENLTKVKDQLCLSESCKKEVKQEAEEAKNQLEAMALKLQXXXX 125

Query: 133 XXXXXXXXXXXXXXXXXXXXXERDRAWQXXXXXXXXXHKLDYAALATAVNEIHQLKIHLE 192
           XXXXXXXXXXXXXXXXXXXXX RDRAWQ         HKLDYAALATAVNEI QLKIHLE
Sbjct: 126 XXXXXXXXXXXXXXXXXXXXXXRDRAWQAELETIQEQHKLDYAALATAVNEIRQLKIHLE 185

Query: 193 MVAQSETKKIKLTDSENNELDSLRIHLAEALCVXXXXXXXXXXXXXXXXXXXXXXXXXXX 252
           MVAQS+TKKIKLTDSENNELD            XXXXXXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 186 MVAQSKTKKIKLTDSENNELDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 245

Query: 253 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKARISVLEELVSKLKTDVANPAVENEK 312
           XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX    SVLEELVSKLKTDVANPAVENEK
Sbjct: 246 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSVLEELVSKLKTDVANPAVENEK 305

Query: 313 QGDHIDEHCCLKTEIEQLKSALEMAETKYHEEQIQNAVKIRTAYEQVEQIKTNMNSKXXX 372
           QGDHIDEHCCLK+EIEQLKSALEMAETKYHEEQIQNAVKIRTAYEQVEQIKTNMNS+   
Sbjct: 306 QGDHIDEHCCLKSEIEQLKSALEMAETKYHEEQIQNAVKIRTAYEQVEQIKTNMNSRESN 365

Query: 373 XXXXXXXXXXXXXXXXXXXXXXXXXXXAISEENEQLHAKIQKNLSSQREYELEREIKELK 432
                                      AISEENEQLH KIQKNLSSQREYELEREIKELK
Sbjct: 366 LEQELKKSKVDIDELKADLMDKETELQAISEENEQLHTKIQKNLSSQREYELEREIKELK 425

Query: 433 DHLAVMKSNLIDKDRQFRSVLDENDTLKLDINKRIMIRAKAIDGTTEVEDTTTVEQEALT 492
           DHLAVMKSNLIDKDRQFRSVLDENDTLKLDINKRIMIRAKA DGTTEVEDTTTVEQEALT
Sbjct: 426 DHLAVMKSNLIDKDRQFRSVLDENDTLKLDINKRIMIRAKANDGTTEVEDTTTVEQEALT 485

Query: 493 RLGVTIEETEKSNKRAARVSEQLEAAQAANVEIETELRRLRVQSDQWRKAAEAAAAMLSA 552
           RLGV +EETEKSNKRAARVSEQLEAAQAANVEIETELRRLRVQSDQWRKAAEAAAAMLSA
Sbjct: 486 RLGVMMEETEKSNKRAARVSEQLEAAQAANVEIETELRRLRVQSDQWRKAAEAAAAMLSA 545

Query: 553 GNNGKFMDRSASLDSSYDSDSAGKVNRSCAEIDDDLLKKKHGNVLKKIGVLWKKQHK 610
           GNNGKFMDRSASLDSSYDSDSAGK+NRSCAEIDDDLLKKKHGNVLKKIGVLWKKQHK
Sbjct: 546 GNNGKFMDRSASLDSSYDSDSAGKMNRSCAEIDDDLLKKKHGNVLKKIGVLWKKQHK 602

BLAST of Carg12303-RA vs. NCBI nr
Match: XP_023521523.1 (interactor of constitutive active ROPs 3-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023521524.1 interactor of constitutive active ROPs 3-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 759.6 bits (1960), Expect = 8.1e-216
Identity = 524/597 (87.77%), Postives = 531/597 (88.94%), Query Frame = 0

Query: 13  NRNSFSEPPQKVSPRVARKLRPASLECDSASSTRTNNRTIKERSPKVVDRKSPRSPASEK 72
           +RNSFSEPPQKVSPRVARKLRPASLECDSASS+RTNNRTIKERSPKVVDRKSPRSPASEK
Sbjct: 6   SRNSFSEPPQKVSPRVARKLRPASLECDSASSSRTNNRTIKERSPKVVDRKSPRSPASEK 65

Query: 73  KRPSKISELESQVCQLQENLTKVKDQLCLSESCKKEVKQEAEEAKNQLEAMALKLQXXXX 132
           KRPSKISELESQVCQLQENLTKVKDQLCLSESCKKEVKQEAEEAK QLEAMALKL XXXX
Sbjct: 66  KRPSKISELESQVCQLQENLTKVKDQLCLSESCKKEVKQEAEEAKTQLEAMALKLXXXXX 125

Query: 133 XXXXXXXXXXXXXXXXXXXXXERDRAWQXXXXXXXXXHKLDYAALATAVNEIHQLKIHLE 192
           XXXXXXXXXXXXXXXXXX   ERDRAWQ         HKLDYAALATAVNEIHQLKIHLE
Sbjct: 126 XXXXXXXXXXXXXXXXXXISLERDRAWQAELETIQEQHKLDYAALATAVNEIHQLKIHLE 185

Query: 193 MVAQSETKKIKLTDSENNELDSLRIHLAEALCVXXXXXXXXXXXXXXXXXXXXXXXXXXX 252
           MVAQS+TKKIKLTDSENN               XXXXXXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 186 MVAQSKTKKIKLTDSENNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 245

Query: 253 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKARISVLEELVSKLKTDVANPAVENEK 312
           XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX    SVLEELVSKLKTDVANPAVENEK
Sbjct: 246 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSVLEELVSKLKTDVANPAVENEK 305

Query: 313 QGDHIDEHCCLKTEIEQLKSALEMAETKYHEEQIQNAVKIRTAYEQVEQIKTNMNSKXXX 372
           QGDHIDEHCCLK+EIEQLKSALEMAETKYHEEQIQNAVKIRTAYEQVEQIKTNMNS+   
Sbjct: 306 QGDHIDEHCCLKSEIEQLKSALEMAETKYHEEQIQNAVKIRTAYEQVEQIKTNMNSRESN 365

Query: 373 XXXXXXXXXXXXXXXXXXXXXXXXXXXAISEENEQLHAKIQKNLSSQREYELEREIKELK 432
                                      AISEENEQLH KIQKNLSSQREYELEREIKELK
Sbjct: 366 LEQELKKSKVDIDELKADLMDKETELQAISEENEQLHTKIQKNLSSQREYELEREIKELK 425

Query: 433 DHLAVMKSNLIDKDRQFRSVLDENDTLKLDINKRIMIRAKAIDGTTEVEDTTTVEQEALT 492
           DHLAVMKSNLIDKDRQFRSVLDENDTLKLDINKRIMIRAKA DGTTEVEDTTTVEQEALT
Sbjct: 426 DHLAVMKSNLIDKDRQFRSVLDENDTLKLDINKRIMIRAKANDGTTEVEDTTTVEQEALT 485

Query: 493 RLGVTIEETEKSNKRAARVSEQLEAAQAANVEIETELRRLRVQSDQWRKAAEAAAAMLSA 552
           RLGV +EETEKSNKRAARVSEQLEAAQAANVEIETELRRLRVQSDQWRKAAEAAAAMLSA
Sbjct: 486 RLGVMMEETEKSNKRAARVSEQLEAAQAANVEIETELRRLRVQSDQWRKAAEAAAAMLSA 545

Query: 553 GNNGKFMDRSASLDSSYDSDSAGKVNRSCAEIDDDLLKKKHGNVLKKIGVLWKKQHK 610
           GNNGKFMDRSASLDSSYDSDSAGK+NRSCAEIDDDLLKKKHGNVLKKIGVLWKKQHK
Sbjct: 546 GNNGKFMDRSASLDSSYDSDSAGKMNRSCAEIDDDLLKKKHGNVLKKIGVLWKKQHK 602

BLAST of Carg12303-RA vs. NCBI nr
Match: XP_022998865.1 (interactor of constitutive active ROPs 3-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 729.6 bits (1882), Expect = 9.0e-207
Identity = 430/597 (72.03%), Postives = 445/597 (74.54%), Query Frame = 0

Query: 13  NRNSFSEPPQKVSPRVARKLRPASLECDSASSTRTNNRTIKERSPKVVDRKSPRSPASEK 72
           +RNSFSEPPQKVSPRVARKLRPAS ECDSASSTRTNNRTIKERSPKVVDRKSPRSPASEK
Sbjct: 6   SRNSFSEPPQKVSPRVARKLRPASPECDSASSTRTNNRTIKERSPKVVDRKSPRSPASEK 65

Query: 73  KRPSKISELESQVCQLQENLTKVKDQLCLSESCKKEVKQEAEEAKNQLEAMALKLQXXXX 132
           K                         LCLSESCKKEVKQEAEEAKNQLEAMALKLQ    
Sbjct: 66  KXXXXXXXXXXXXXXXXXXXXXXXXXLCLSESCKKEVKQEAEEAKNQLEAMALKLQESQE 125

Query: 133 XXXXXXXXXXXXXXXXXXXXXERDRAWQXXXXXXXXXHKLDYAALATAVNEIHQLKIHLE 192
                                ERDRAWQ          KLDYAALATAVNEIHQ KIHLE
Sbjct: 126 QVLELSTSNEAHVIELQEISLERDRAWQSELETIQEQQKLDYAALATAVNEIHQQKIHLE 185

Query: 193 MVAQSETKKIKLTDSENNELDSLRIHLAEALCVXXXXXXXXXXXXXXXXXXXXXXXXXXX 252
           MVAQS+TKKIKLTDSENNELDSLRIHLA+ALCV                           
Sbjct: 186 MVAQSKTKKIKLTDSENNELDSLRIHLADALCVVERMKNELESCRESEAQARLLANETLA 245

Query: 253 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKARISVLEELVSKLKTDVANPAVENEK 312
           XXXX                             KARISVLEELVSKLKTDVANPAVENEK
Sbjct: 246 XXXXAKKRVEELRYDGMEAMKGYHSIASELDESKARISVLEELVSKLKTDVANPAVENEK 305

Query: 313 QGDHIDEHCCLKTEIEQLKSALEMAETKYHEEQIQNAVKIRTAYEQVEQIKTNMNSKXXX 372
           +GDHIDEHCCLK+EIEQLKSALEMAETKYHEEQIQNAVKIRTAYEQVEQIKTNMNS+   
Sbjct: 306 KGDHIDEHCCLKSEIEQLKSALEMAETKYHEEQIQNAVKIRTAYEQVEQIKTNMNSRESN 365

Query: 373 XXXXXXXXXXXXXXXXXXXXXXXXXXXAISEENEQLHAKIQKNLSSQREYELEREIKELK 432
                                      AISEENEQLHAKIQKNLSSQREYELEREIKELK
Sbjct: 366 LEQELKKSKVDIDELKADLMDKETELQAISEENEQLHAKIQKNLSSQREYELEREIKELK 425

Query: 433 DHLAVMKSNLIDKDRQFRSVLDENDTLKLDINKRIMIRAKAIDGTTEVEDTTTVEQEALT 492
           DHLAVMKSNLIDKDRQFRS+LDENDTLK DINKRIMIRAKA DGTTEV++ TTVEQEALT
Sbjct: 426 DHLAVMKSNLIDKDRQFRSILDENDTLKFDINKRIMIRAKASDGTTEVDNATTVEQEALT 485

Query: 493 RLGVTIEETEKSNKRAARVSEQLEAAQAANVEIETELRRLRVQSDQWRKAAEAAAAMLSA 552
           RLGV +EETEKSNKRAARVSEQLEAAQAANVE+ETELRRLRVQSDQWRKAAEAAAAMLSA
Sbjct: 486 RLGVMMEETEKSNKRAARVSEQLEAAQAANVEMETELRRLRVQSDQWRKAAEAAAAMLSA 545

Query: 553 GNNGKFMDRSASLDSSYDSDSAGKVNRSCAEIDDDLLKKKHGNVLKKIGVLWKKQHK 610
           GNNGKFMD+SASLDSSY+SD+AGK+NRSCAEIDDDLLKKKHGNVLKKIGVLWKKQHK
Sbjct: 546 GNNGKFMDQSASLDSSYNSDAAGKMNRSCAEIDDDLLKKKHGNVLKKIGVLWKKQHK 602

BLAST of Carg12303-RA vs. NCBI nr
Match: XP_022949430.1 (interactor of constitutive active ROPs 3-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 611.3 bits (1575), Expect = 3.6e-171
Identity = 401/487 (82.34%), Postives = 406/487 (83.37%), Query Frame = 0

Query: 123 MALKLQXXXXXXXXXXXXXXXXXXXXXXXXXERDRAWQXXXXXXXXXHKLDYAALATAVN 182
           MALKLQ                         ERDRAWQ         HKLDYAALA+AVN
Sbjct: 1   MALKLQESQEQVLELSTSKEAHVIELQEISLERDRAWQAELETIQEQHKLDYAALASAVN 60

Query: 183 EIHQLKIHLEMVAQSETKKIKLTDSENNELDSLRIHLAEALCVXXXXXXXXXXXXXXXXX 242
           EIHQLKIHLEMVAQSETKKI LTDSENNELDSLR HLAEALC XXXXXXXXXXXXXXXXX
Sbjct: 61  EIHQLKIHLEMVAQSETKKINLTDSENNELDSLRTHLAEALCXXXXXXXXXXXXXXXXXX 120

Query: 243 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKARISVLEELVSKLKTD 302
           XXXXXXXXXXXXXXXXXXXXXXXXXXXXXX             KARISVLEELVSKLKTD
Sbjct: 121 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKGYHSIASELDESKARISVLEELVSKLKTD 180

Query: 303 VANPAVENEKQGDHIDEHCCLKTEIEQLKSALEMAETKYHEEQIQNAVKIRTAYEQVEQI 362
           VANPAVENEKQGDHIDEHCCLK+EIEQLKSALEMAETKYHEEQIQNAVK+RTAYEQVEQI
Sbjct: 181 VANPAVENEKQGDHIDEHCCLKSEIEQLKSALEMAETKYHEEQIQNAVKVRTAYEQVEQI 240

Query: 363 KTNMNSKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAISEENEQLHAKIQKNLSSQREY 422
           KTNMNS+                              AISEENEQLHAKIQKNLSSQREY
Sbjct: 241 KTNMNSRESNLEQELKKSKVDIDELKADLMDKETELQAISEENEQLHAKIQKNLSSQREY 300

Query: 423 ELEREIKELKDHLAVMKSNLIDKDRQFRSVLDENDTLKLDINKRIMIRAKAIDGTTEVED 482
           ELEREIKELKDHLAVMKSNLIDKDRQFRSVLDENDTLKLDINKRIMIRAKA DGTTEVED
Sbjct: 301 ELEREIKELKDHLAVMKSNLIDKDRQFRSVLDENDTLKLDINKRIMIRAKASDGTTEVED 360

Query: 483 TTTVEQEALTRLGVTIEETEKSNKRAARVSEQLEAAQAANVEIETELRRLRVQSDQWRKA 542
           TTTVEQEALTRLGVT+EETEKSNKRAARVSEQLEAAQAANVEIETELRRLRVQSDQWRKA
Sbjct: 361 TTTVEQEALTRLGVTMEETEKSNKRAARVSEQLEAAQAANVEIETELRRLRVQSDQWRKA 420

Query: 543 AEAAAAMLSAGNNGKFMDRSASLDSSYDSDSAGKVNRSCAEIDDDLLKKKHGNVLKKIGV 602
           AEAAAAMLSAGNNGKFMDRSASLDSSYDSDSAGKVNRSCAEIDDDLLKKKHGNVLKKIGV
Sbjct: 421 AEAAAAMLSAGNNGKFMDRSASLDSSYDSDSAGKVNRSCAEIDDDLLKKKHGNVLKKIGV 480

Query: 603 LWKKQHK 610
           LWKKQHK
Sbjct: 481 LWKKQHK 487

BLAST of Carg12303-RA vs. TAIR10
Match: AT5G60210.1 (ROP interactive partner 5)

HSP 1 Score: 171.8 bits (434), Expect = 1.3e-42
Identity = 216/613 (35.24%), Postives = 286/613 (46.66%), Query Frame = 0

Query: 14  RNSFSEPPQKVSPRVARKLRPASLECDSASS-TRTNNRTIKERSPKVVDRKSPRSPASEK 73
           RN   + P+KVSPR AR L+ A+LE +S+SS     NRT K++SP V++R+SPRSP SEK
Sbjct: 7   RNGSPDVPKKVSPRAARPLKIAALEPESSSSPISATNRTPKDKSPNVLNRRSPRSPVSEK 66

Query: 74  KRPSKISELESQVCQLQENLTKVKDQL--CLSESCKKEVKQEAEEAKNQLEAMALKLQXX 133
           KRPS+I+ELE  V QLQE L K KDQ+                               XX
Sbjct: 67  KRPSRITELELLVSQLQEELKKAKDQISXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 126

Query: 134 XXXXXXXXXXXXXXXXXXXXXXXERDRAWQXXXXXXXXXHKLDYAALATAVNEIHQLKIH 193
           XXXXXXXXXXXX            ++  W+            + A LA   +EI QLK+ 
Sbjct: 127 XXXXXXXXXXXXDKTGSLVFQSVSQECDWEFSATAG------ERAGLAAVAHEIRQLKLQ 186

Query: 194 LEMVAQSETKKIKLTDSENNELDSLRIHLAEALCVXXXXXXXXXXXXXXXXXXXXXXXXX 253
           +EMVA SE   +K  +  N+E+  LR +L + L                           
Sbjct: 187 IEMVASSEAGHVKQAELYNSEVQLLRGNLMDTLFHVENFRNQLKDCEISEAETEALATET 246

Query: 254 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKARISVLEELVSKLKTDVANPA-VE 313
                       XXXXXXXXXXXXXXXXXXXXX  K+R+  LE LV+KL+    NPA +E
Sbjct: 247 LRQLENAKKAVEXXXXXXXXXXXXXXXXXXXXXQSKSRMVWLEALVNKLQN---NPADLE 306

Query: 314 NEK------------QGDHIDEH-CCLKTEIEQLKSALEMAETKYHEEQIQNAVKIRTAY 373
           N +            + + +DE    L+ E+E+L++ALE ++ K  E  ++ + ++R   
Sbjct: 307 NHEILLKDYESLRRGESNEMDEEVSSLRCEVERLRAALEASDKKDQEGNVEASSRLRIQA 366

Query: 374 EQVEQIKTNMNSKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAISEENEQLHAKIQKNL 433
           E   ++K   +                                 ISEE +    K+ KN 
Sbjct: 367 ELQSELKIAKSE---------------IDELKARLMDKETELQFISEERDNFSMKLMKNQ 426

Query: 434 SSQREYELEREIKELKDHLAVMKSNLIDKDRQFRSVLDENDTLKLDINKRIMIRAKAIDG 493
                                                                       
Sbjct: 427 K--------------------XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 486

Query: 494 TTEVEDTTTVEQEALTRLGVTIEETEKSNKRAARVSEQLEAAQAANVEIETELRRLRVQS 553
                      Q+A  +LG+ +EE +KS+K+A RV+EQLEA QA+N E+ETELR+L+VQS
Sbjct: 487 XXXXXXXXXXXQDAFLKLGIAMEEADKSSKKAVRVTEQLEATQASNSEMETELRKLKVQS 546

Query: 554 DQWRKAAEAAAAMLSAGNNGKFMDRSASLDSSYDSDSAGKVNRSCAEIDDDLLKKKHGNV 610
           +QWRKAAEAA AMLSAGNNGKF +     +S Y  D           IDD+L KKK+GNV
Sbjct: 547 NQWRKAAEAATAMLSAGNNGKFAENYNQTNSPYSED-----------IDDELTKKKNGNV 564

BLAST of Carg12303-RA vs. TAIR10
Match: AT2G37080.1 (ROP interactive partner 3)

HSP 1 Score: 128.3 bits (321), Expect = 1.7e-29
Identity = 150/558 (26.88%), Postives = 217/558 (38.89%), Query Frame = 0

Query: 71  EKKRPSKISELESQVCQLQENLTKVKDQLCLSESCKKEVKQEAEEAKNQLEAMALKLQXX 130
           +KKR  K  EL SQ+ QLQE L K K+QL  SE+ K             L  +       
Sbjct: 69  QKKRTGKTPELASQISQLQEELKKAKEQLSASEALKXXXXXXXXXXXXXLMEI------- 128

Query: 131 XXXXXXXXXXXXXXXXXXXXXXXERDRAWQXXXXXXXXXHKLDYAALATAVNEIHQLKIH 190
                                  ERD+AWQ         H +D AAL++ +NE+ +LK  
Sbjct: 129 -------NASEDSRIDELRKLSQERDKAWQSELEAMQRQHAMDSAALSSTMNEVQKLKAQ 188

Query: 191 LEMVAQSETKKIKLTDSENNELDSLRIHLAEALCVXXXXXXXXXXXXXXXXXXXXXXXXX 250
           L               SE+  +++LR+ L E L +                         
Sbjct: 189 L---------------SESENVENLRMELNETLSLVEKLRGELFDAKEGEAQAHEIVSGT 248

Query: 251 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKARISVLEELVSKLKTD---VANPA 310
                                              K+ +  LE+LV +L+ +     N  
Sbjct: 249 EKQLEIANLTLEMLRSDGMKMSEACNSLTTELEQSKSEVRSLEQLVRQLEEEDEARGNAN 308

Query: 311 VENEKQGDHIDEHCCLKTEIEQLKSALEMAETKYHEEQIQNAVKIRTAYEQVEQIKTNMN 370
            ++    +  +E    + EI QLKSA+E+ E +YHEE IQ+ ++IRTAYEQV+++K+   
Sbjct: 309 GDSSSVEELKEEINVARQEISQLKSAVEVTERRYHEEYIQSTLQIRTAYEQVDEVKSGYA 368

Query: 371 SKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAISEENEQLHAKIQ---------KNLSS 430
            +                               + +ENE L++KI+         +N  +
Sbjct: 369 QREAELGEELKKTKAERDSLHERLMDKEAKLRILVDENEILNSKIKEKEEVYLNLENSLN 428

Query: 431 QREYELEREIKELKDHLAVMKSNLIDKDRQFRSVLDENDTLKLDINKRIMIRAKAIDGTT 490
           Q E E   E+K+L+                                        AID   
Sbjct: 429 QNEPEDTGELKKLESDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAID--- 488

Query: 491 EVEDTTTVEQEALTRLGVTIEETEKSNKRAARVSEQLEAAQAANVEIETELRRLRVQSDQ 550
                     EAL +LG   EE +KS KRA   +EQL AAQ  N E+E ELRRL+VQ DQ
Sbjct: 489 ----------EALAKLGSLTEEADKSGKRAENATEQLGAAQVTNTELEAELRRLKVQCDQ 548

Query: 551 WRKA-----AEAAAAMLSAGNNGKFMDRSASLDSSYDSDSAGKVNRSCAEIDDDLL--KK 610
           WRKA                  GK+++R+ SL+S     +   ++    E DD+L   KK
Sbjct: 549 WRKAXXXXXXXXXXXXXXXXXXGKYVERTGSLESPLRRRNV-NMSPYMGETDDELSSPKK 583

BLAST of Carg12303-RA vs. TAIR10
Match: AT1G78430.1 (ROP interactive partner 2)

HSP 1 Score: 53.1 bits (126), Expect = 6.8e-07
Identity = 44/109 (40.37%), Postives = 63/109 (57.80%), Query Frame = 0

Query: 18  SEPPQKVSPRVARKLRPASLECDSASSTRTNNRTIKERSPKV-VDRKSPRS------PAS 77
           SE PQ+ SPR+   L        ++S     +R I +RSPK+ +DR+SPRS      P S
Sbjct: 9   SELPQRQSPRLRTSL------LSTSSDPHHLSRPITDRSPKLGLDRRSPRSGGPHTDPLS 68

Query: 78  EKKRPSKISELESQVCQLQENLTKVKDQLCLSESCKKEVKQEAEEAKNQ 120
           +KK  S+IS LESQ+ Q QE L  +K QL  +E+ KK  ++E    K++
Sbjct: 69  QKKLGSRISGLESQLGQAQEELRLLKQQLAKAEAAKKRAQEELHRKKSK 111

BLAST of Carg12303-RA vs. TAIR10
Match: AT1G17140.1 (interactor of constitutive active rops 1)

HSP 1 Score: 45.4 bits (106), Expect = 1.4e-04
Identity = 41/110 (37.27%), Postives = 61/110 (55.45%), Query Frame = 0

Query: 23  KVSPRVARKLRPASLECDSASSTR--TNNRTIKERSPKVVDRKSPRS------PASEKKR 82
           ++S R A +LR +S   DS  S R  T +++ K      VDRKSPRS      P  +KK 
Sbjct: 8   ELSQRQAPRLRSSSSTSDSNHSNRLITTDQSFKPG----VDRKSPRSGGPNSDPLGQKKL 67

Query: 83  PSKISELESQVCQLQENLTKVKDQLCLSESCKKEVKQEAEEAKNQLEAMA 125
             +IS+LESQ+ Q QE L  +K+QL  +E+ KK+ + E  +   +   +A
Sbjct: 68  GGRISDLESQLGQAQEELRLLKEQLANAEAVKKQAQDELHKKSKKPNPLA 113

BLAST of Carg12303-RA vs. Swiss-Prot
Match: sp|Q9LSS5|ICR3_ARATH (Interactor of constitutive active ROPs 3 OS=Arabidopsis thaliana OX=3702 GN=ICR3 PE=1 SV=1)

HSP 1 Score: 171.8 bits (434), Expect = 2.4e-41
Identity = 216/613 (35.24%), Postives = 286/613 (46.66%), Query Frame = 0

Query: 14  RNSFSEPPQKVSPRVARKLRPASLECDSASS-TRTNNRTIKERSPKVVDRKSPRSPASEK 73
           RN   + P+KVSPR AR L+ A+LE +S+SS     NRT K++SP V++R+SPRSP SEK
Sbjct: 7   RNGSPDVPKKVSPRAARPLKIAALEPESSSSPISATNRTPKDKSPNVLNRRSPRSPVSEK 66

Query: 74  KRPSKISELESQVCQLQENLTKVKDQL--CLSESCKKEVKQEAEEAKNQLEAMALKLQXX 133
           KRPS+I+ELE  V QLQE L K KDQ+                               XX
Sbjct: 67  KRPSRITELELLVSQLQEELKKAKDQISXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 126

Query: 134 XXXXXXXXXXXXXXXXXXXXXXXERDRAWQXXXXXXXXXHKLDYAALATAVNEIHQLKIH 193
           XXXXXXXXXXXX            ++  W+            + A LA   +EI QLK+ 
Sbjct: 127 XXXXXXXXXXXXDKTGSLVFQSVSQECDWEFSATAG------ERAGLAAVAHEIRQLKLQ 186

Query: 194 LEMVAQSETKKIKLTDSENNELDSLRIHLAEALCVXXXXXXXXXXXXXXXXXXXXXXXXX 253
           +EMVA SE   +K  +  N+E+  LR +L + L                           
Sbjct: 187 IEMVASSEAGHVKQAELYNSEVQLLRGNLMDTLFHVENFRNQLKDCEISEAETEALATET 246

Query: 254 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKARISVLEELVSKLKTDVANPA-VE 313
                       XXXXXXXXXXXXXXXXXXXXX  K+R+  LE LV+KL+    NPA +E
Sbjct: 247 LRQLENAKKAVEXXXXXXXXXXXXXXXXXXXXXQSKSRMVWLEALVNKLQN---NPADLE 306

Query: 314 NEK------------QGDHIDEH-CCLKTEIEQLKSALEMAETKYHEEQIQNAVKIRTAY 373
           N +            + + +DE    L+ E+E+L++ALE ++ K  E  ++ + ++R   
Sbjct: 307 NHEILLKDYESLRRGESNEMDEEVSSLRCEVERLRAALEASDKKDQEGNVEASSRLRIQA 366

Query: 374 EQVEQIKTNMNSKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAISEENEQLHAKIQKNL 433
           E   ++K   +                                 ISEE +    K+ KN 
Sbjct: 367 ELQSELKIAKSE---------------IDELKARLMDKETELQFISEERDNFSMKLMKNQ 426

Query: 434 SSQREYELEREIKELKDHLAVMKSNLIDKDRQFRSVLDENDTLKLDINKRIMIRAKAIDG 493
                                                                       
Sbjct: 427 K--------------------XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 486

Query: 494 TTEVEDTTTVEQEALTRLGVTIEETEKSNKRAARVSEQLEAAQAANVEIETELRRLRVQS 553
                      Q+A  +LG+ +EE +KS+K+A RV+EQLEA QA+N E+ETELR+L+VQS
Sbjct: 487 XXXXXXXXXXXQDAFLKLGIAMEEADKSSKKAVRVTEQLEATQASNSEMETELRKLKVQS 546

Query: 554 DQWRKAAEAAAAMLSAGNNGKFMDRSASLDSSYDSDSAGKVNRSCAEIDDDLLKKKHGNV 610
           +QWRKAAEAA AMLSAGNNGKF +     +S Y  D           IDD+L KKK+GNV
Sbjct: 547 NQWRKAAEAATAMLSAGNNGKFAENYNQTNSPYSED-----------IDDELTKKKNGNV 564

BLAST of Carg12303-RA vs. Swiss-Prot
Match: sp|Q9ZQC5|ICR2_ARATH (Interactor of constitutive active ROPs 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ICR2 PE=1 SV=1)

HSP 1 Score: 128.3 bits (321), Expect = 3.0e-28
Identity = 150/558 (26.88%), Postives = 217/558 (38.89%), Query Frame = 0

Query: 71  EKKRPSKISELESQVCQLQENLTKVKDQLCLSESCKKEVKQEAEEAKNQLEAMALKLQXX 130
           +KKR  K  EL SQ+ QLQE L K K+QL  SE+ K             L  +       
Sbjct: 69  QKKRTGKTPELASQISQLQEELKKAKEQLSASEALKXXXXXXXXXXXXXLMEI------- 128

Query: 131 XXXXXXXXXXXXXXXXXXXXXXXERDRAWQXXXXXXXXXHKLDYAALATAVNEIHQLKIH 190
                                  ERD+AWQ         H +D AAL++ +NE+ +LK  
Sbjct: 129 -------NASEDSRIDELRKLSQERDKAWQSELEAMQRQHAMDSAALSSTMNEVQKLKAQ 188

Query: 191 LEMVAQSETKKIKLTDSENNELDSLRIHLAEALCVXXXXXXXXXXXXXXXXXXXXXXXXX 250
           L               SE+  +++LR+ L E L +                         
Sbjct: 189 L---------------SESENVENLRMELNETLSLVEKLRGELFDAKEGEAQAHEIVSGT 248

Query: 251 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKARISVLEELVSKLKTD---VANPA 310
                                              K+ +  LE+LV +L+ +     N  
Sbjct: 249 EKQLEIANLTLEMLRSDGMKMSEACNSLTTELEQSKSEVRSLEQLVRQLEEEDEARGNAN 308

Query: 311 VENEKQGDHIDEHCCLKTEIEQLKSALEMAETKYHEEQIQNAVKIRTAYEQVEQIKTNMN 370
            ++    +  +E    + EI QLKSA+E+ E +YHEE IQ+ ++IRTAYEQV+++K+   
Sbjct: 309 GDSSSVEELKEEINVARQEISQLKSAVEVTERRYHEEYIQSTLQIRTAYEQVDEVKSGYA 368

Query: 371 SKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAISEENEQLHAKIQ---------KNLSS 430
            +                               + +ENE L++KI+         +N  +
Sbjct: 369 QREAELGEELKKTKAERDSLHERLMDKEAKLRILVDENEILNSKIKEKEEVYLNLENSLN 428

Query: 431 QREYELEREIKELKDHLAVMKSNLIDKDRQFRSVLDENDTLKLDINKRIMIRAKAIDGTT 490
           Q E E   E+K+L+                                        AID   
Sbjct: 429 QNEPEDTGELKKLESDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAID--- 488

Query: 491 EVEDTTTVEQEALTRLGVTIEETEKSNKRAARVSEQLEAAQAANVEIETELRRLRVQSDQ 550
                     EAL +LG   EE +KS KRA   +EQL AAQ  N E+E ELRRL+VQ DQ
Sbjct: 489 ----------EALAKLGSLTEEADKSGKRAENATEQLGAAQVTNTELEAELRRLKVQCDQ 548

Query: 551 WRKA-----AEAAAAMLSAGNNGKFMDRSASLDSSYDSDSAGKVNRSCAEIDDDLL--KK 610
           WRKA                  GK+++R+ SL+S     +   ++    E DD+L   KK
Sbjct: 549 WRKAXXXXXXXXXXXXXXXXXXGKYVERTGSLESPLRRRNV-NMSPYMGETDDELSSPKK 583

BLAST of Carg12303-RA vs. Swiss-Prot
Match: sp|Q9M9F9|ICR4_ARATH (Interactor of constitutive active ROPs 4 OS=Arabidopsis thaliana OX=3702 GN=ICR4 PE=1 SV=2)

HSP 1 Score: 53.1 bits (126), Expect = 1.2e-05
Identity = 44/109 (40.37%), Postives = 63/109 (57.80%), Query Frame = 0

Query: 18  SEPPQKVSPRVARKLRPASLECDSASSTRTNNRTIKERSPKV-VDRKSPRS------PAS 77
           SE PQ+ SPR+   L        ++S     +R I +RSPK+ +DR+SPRS      P S
Sbjct: 9   SELPQRQSPRLRTSL------LSTSSDPHHLSRPITDRSPKLGLDRRSPRSGGPHTDPLS 68

Query: 78  EKKRPSKISELESQVCQLQENLTKVKDQLCLSESCKKEVKQEAEEAKNQ 120
           +KK  S+IS LESQ+ Q QE L  +K QL  +E+ KK  ++E    K++
Sbjct: 69  QKKLGSRISGLESQLGQAQEELRLLKQQLAKAEAAKKRAQEELHRKKSK 111

BLAST of Carg12303-RA vs. TrEMBL
Match: tr|A0A0A0LVB5|A0A0A0LVB5_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G089500 PE=4 SV=1)

HSP 1 Score: 561.6 bits (1446), Expect = 2.1e-156
Identity = 371/610 (60.82%), Postives = 415/610 (68.03%), Query Frame = 0

Query: 13  NRNSFSEPPQKVSPRVARKLRPASLECDS-ASSTRTNNRTIKERSPKVVDRKSPRSPA-S 72
           +RNS  E PQK SPRVARKLRP +LECDS +SS + NNR IKERSPKV+DRKSPRSPA  
Sbjct: 6   SRNSLPEAPQKASPRVARKLRPTALECDSTSSSNQVNNRIIKERSPKVIDRKSPRSPAXX 65

Query: 73  EKKRPSKISELESQVCQLQENLTKVKDQLCLSESCKKEVKQEAEEAKNQLEAMALKLQXX 132
                SKISELESQV QLQE+LT VKDQLCLSESCKKE KQ+AEEAK+QL AM LKLQ  
Sbjct: 66  XXXXXSKISELESQVSQLQEDLTIVKDQLCLSESCKKEFKQDAEEAKDQLVAMTLKLQES 125

Query: 133 XXXXXXXXXXXXXXXXXXXXXXXERDRAWQXXXXXXXXXHKLDYAALATAVNEIHQLKIH 192
                                  +RD+AWQ         HKLDY+ALA+AVNEI QLKI 
Sbjct: 126 EKLVLELSASEEARVIELQQISQDRDQAWQSELEAIQEQHKLDYSALASAVNEIQQLKIQ 185

Query: 193 LEMVAQSETKKIKLTDSENNELDSLRIHLAEALCVXXXXXXXXXXXXXXXXXXXXXXXXX 252
           LEMVAQSETKK +  DS N ELDS R  LAE LC+                         
Sbjct: 186 LEMVAQSETKKTEHADSANKELDSFRSKLAETLCLMERMEHELKNCRESEAQAQLLARET 245

Query: 253 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKARISVLEELVSKLKTDVAN----- 312
                                              KAR+++LE+LV KL+TDVAN     
Sbjct: 246 LAQLEDAKKRLEELRFDGMEAKKAYNAIALELDESKARVNILEKLVGKLETDVANASSNL 305

Query: 313 ---PAVENEKQ--GDHIDEHCCLKTEIEQLKSALEMAETKYHEEQIQNAVKIRTAYEQVE 372
              PAVENEK+   DHI+E C L++EIEQLK+ALE+AE KYHE+QIQ+AV+I+ AYEQVE
Sbjct: 306 LQHPAVENEKKRGEDHIEELCSLRSEIEQLKTALEVAEAKYHEDQIQSAVQIKMAYEQVE 365

Query: 373 QIKTNMNSKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAISEENEQLHAKIQKNLSSQR 432
           QIK+N NS+             XXXXXXXXXXXXXXXXX       QL AKIQKNLSSQR
Sbjct: 366 QIKSNKNSREFNLEQELKKFKAXXXXXXXXXXXXXXXXXXXXXXXXQLQAKIQKNLSSQR 425

Query: 433 EYELEREIKELKDHLAVMKSNLIDKDRQFRSVLDENDTLKLDINKRIMIRAKAIDGTTEV 492
           EYELEREIK          S+L DK+R + S+ DEN+ LKL+INKRIMIRAKA +GTTEV
Sbjct: 426 EYELEREIKXXXXXXXXXXSSLTDKERDYHSISDENEMLKLEINKRIMIRAKASNGTTEV 485

Query: 493 EDTTTVEQEALTRLGVTIEETEKSNKRAARVSEQLEAAQAANVEIETELRRLRVQSDQWR 552
           ED T  EQ+AL RLG+ +EE +KSNKRAARVSEQLEAAQAAN EIETELRRLRVQSDQWR
Sbjct: 486 EDATAAEQDALIRLGIVMEEADKSNKRAARVSEQLEAAQAANAEIETELRRLRVQSDQWR 545

Query: 553 KAAEAAAAMLSAGNNGKFMDRSASLDSSYDSDSAGKVNRS-CAEIDDDLLKKKHGNVLKK 610
           KAAEAAAAMLSAGNNGK MDRS SLDSSYDS +AGK N S C EIDDDLLKKK+GNVLKK
Sbjct: 546 KAAEAAAAMLSAGNNGKLMDRSGSLDSSYDS-AAGKFNGSYCEEIDDDLLKKKNGNVLKK 605

BLAST of Carg12303-RA vs. TrEMBL
Match: tr|A0A1S3CJE4|A0A1S3CJE4_CUCME (interactor of constitutive active ROPs 3-like OS=Cucumis melo OX=3656 GN=LOC103501623 PE=4 SV=1)

HSP 1 Score: 544.7 bits (1402), Expect = 2.7e-151
Identity = 378/610 (61.97%), Postives = 419/610 (68.69%), Query Frame = 0

Query: 13  NRNSFSEPPQKVSPRVARKLRPASLECDS-ASSTRTNNRTIKERSPKVVDRKSPRSPAS- 72
           +RNS  E PQK SPRVARKLRP +LECDS +SS + NNR IKERSPKV+DRKSPRSPAS 
Sbjct: 6   SRNSLPEAPQKASPRVARKLRPTALECDSTSSSNQVNNRIIKERSPKVIDRKSPRSPASX 65

Query: 73  EKKRPSKISELESQVCQLQENLTKVKDQLCLSESCKKEVKQEAEEAKNQLEAMALKLQXX 132
                SKISELESQV QLQE+LT VKDQLCLSESCKK  KQ+AEEAKNQL AM LKLQ  
Sbjct: 66  XXXXXSKISELESQVSQLQEDLTIVKDQLCLSESCKKAFKQDAEEAKNQLVAMTLKLQES 125

Query: 133 XXXXXXXXXXXXXXXXXXXXXXXERDRAWQXXXXXXXXXHKLDYAALATAVNEIHQLKIH 192
                                  +RD+AWQ         HKLDYAALA+AVNEI QLKI 
Sbjct: 126 EKLVLQLSASEEARVIELQQISQDRDQAWQSELEAIQEQHKLDYAALASAVNEIQQLKIQ 185

Query: 193 LEMVAQSETKKIKLTDSENNELDSLRIHLAEALCVXXXXXXXXXXXXXXXXXXXXXXXXX 252
           LEMVA+SETKK +  DS NNELDSLR  LAE LC+                         
Sbjct: 186 LEMVAKSETKKTEHADSANNELDSLRSKLAETLCLVERMEHELKNCRESEAQAQLLARET 245

Query: 253 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKARISVLEELVSKLKTDVA------ 312
                                              KAR+++LE+LV KL+TDVA      
Sbjct: 246 LAQLEDAKKRVEELRFDGTEAKKAYNAIALELDESKARVNLLEKLVGKLETDVANASSNL 305

Query: 313 --NPAVENEKQ--GDHIDEHCCLKTEIEQLKSALEMAETKYHEEQIQNAVKIRTAYEQVE 372
             NP VEN K+   DHI+E C LK+EIEQLK+ALE+AETKYHE+QIQ++V+I+ AYEQVE
Sbjct: 306 LQNPPVENNKKRGEDHIEELCSLKSEIEQLKTALEVAETKYHEDQIQSSVQIKMAYEQVE 365

Query: 373 QIKTNMNSKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAISEENEQLHAKIQKNLSSQR 432
           QIK NMNS XXXXXXXXXXXXXXXXXXXXXXXXXXXXXX           KIQKNLSS+R
Sbjct: 366 QIKFNMNSXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKIQKNLSSER 425

Query: 433 EYELEREIKELKDHLAVMKSNLIDKDRQFRSVLDENDTLKLDINKRIMIRAKAIDGTTEV 492
           EYEL                   DK+R +RS+ DEN+ LKL+INKRIMIRAKA +GTTEV
Sbjct: 426 EYELXXXXXXXXXXXXXXXXXXTDKERDYRSISDENEMLKLEINKRIMIRAKASNGTTEV 485

Query: 493 EDTTTVEQEALTRLGVTIEETEKSNKRAARVSEQLEAAQAANVEIETELRRLRVQSDQWR 552
           ED T  EQ+AL RLG+ +EE +KSNKRAARVSEQLEAAQAAN EIETELRRLRVQSDQWR
Sbjct: 486 EDATAAEQDALIRLGIVMEEADKSNKRAARVSEQLEAAQAANAEIETELRRLRVQSDQWR 545

Query: 553 KAAEAAAAMLSAGNNGKFMDRSASLDSSYDSDSAGKVNRS-CAEIDDDLLKKKHGNVLKK 610
           KAAEAAAAMLSAGNNGKFMDRS SLDSSYDS +AGK N S C EIDDDLLKKK+GNVLKK
Sbjct: 546 KAAEAAAAMLSAGNNGKFMDRSGSLDSSYDS-AAGKFNGSYCEEIDDDLLKKKNGNVLKK 605

BLAST of Carg12303-RA vs. TrEMBL
Match: tr|A0A151SUZ5|A0A151SUZ5_CAJCA (Interactor of constitutive active ROPs 3 OS=Cajanus cajan OX=3821 GN=KK1_014035 PE=4 SV=1)

HSP 1 Score: 360.9 bits (925), Expect = 5.6e-96
Identity = 252/596 (42.28%), Postives = 354/596 (59.40%), Query Frame = 0

Query: 14  RNSFSEPPQKVSPRVARKLRPASLECDSASSTRTNNRTIKERSPKVVDRKSPRSPASEKK 73
           R+S SE PQKVSPR  R+LRP +L  D+ASS    N+T KERSPK+ DR+SPRSPA E+K
Sbjct: 7   RSSSSEAPQKVSPRGGRQLRPTTL--DTASSLNQANKTSKERSPKITDRRSPRSPAPERK 66

Query: 74  RPSKISELESQVCQLQENLTKVKDQLCLSESCKKEVKQEAEEAKNQLEAMALKLQXXXXX 133
           RPS+ISELESQ+ QL+++L  V++QL LS+S KK+ +Q+AEE+K QL ++++KL+     
Sbjct: 67  RPSRISELESQISQLKDDLKVVRNQLSLSDSGKKQAQQDAEESKKQLASLSVKLEDSQQQ 126

Query: 134 XXXXXXXXXXXXXXXXXXXXERDRAWQXXXXXXXXXHKLDYAALATAVNEIHQLKIHLEM 193
                               E D+AWQ            + AAL +A+NEI QLK+ LE+
Sbjct: 127 LLKFSAAEQARVIELQKTIEEHDKAWQSELRAAQQKLSDNTAALTSAINEIQQLKVQLEL 186

Query: 194 VAQSETKKIKLTDSENNELDSLRIHLAEALCVXXXXXXXXXXXXXXXXXXXXXXXXXXXX 253
           VA  E    ++ +S + EL +L+ +LAE+L +                            
Sbjct: 187 VANCENAHTQIAESSDVELLNLKDNLAESLSL-VENMKNQLRDSKESVQAQALVNETLRQ 246

Query: 254 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKARISVLEELVSKLKTDVANPAVENEKQ 313
                                           +AR++ LE LVSKL+  + +    N   
Sbjct: 247 LEAAKRTVEFLRADAAKAVHGYNSAALELDQSRARVNSLEALVSKLEFGLISNKSNNSV- 306

Query: 314 GDHIDEHCCLKTEIEQLKSALEMAETKYHEEQIQNAVKIRTAYEQVEQIKTNMNSKXXXX 373
            +  DE   L++E+ QL+ A+E AE KY EEQI+ +VKI  AYE +EQI +  + +    
Sbjct: 307 -NLADEIYSLRSEVGQLRYAIETAEAKYREEQIECSVKITKAYELIEQITSESSQRESEL 366

Query: 374 XXXXXXXXXXXXXXXXXXXXXXXXXXAISEENEQLHAKIQKNLSSQREYELEREIKELKD 433
                          XXXXXXXX    I EENE+L+ +++KN+SSQRE+EL +E+++L +
Sbjct: 367 EAELKRKKTDIEELKXXXXXXXXELQGIVEENEKLNLQLEKNMSSQREHELRKELRKLDE 426

Query: 434 HLAVMKSNLIDKDRQFRSVLDENDTLKLDINKRIMIRAKAIDGTTEVEDTTTVEQEALTR 493
            +A +K++L+DK+   +S+ +EN+ LKL+I+KR            E+E     E+EA+ R
Sbjct: 427 CVAGLKADLMDKETTLQSISEENEMLKLEISKRY--------ARAELEAAKAAEREAVMR 486

Query: 494 LGVTIEETEKSNKRAARVSEQLEAAQAANVEIETELRRLRVQSDQWRKAAEAAAAMLSAG 553
           LG+ +E+ E+SN++A RV+EQLEA+QAAN  IE ELRRL+VQSDQWRKAAEAAAAMLSAG
Sbjct: 487 LGIVMEDAERSNRKAVRVAEQLEASQAANSIIEAELRRLKVQSDQWRKAAEAAAAMLSAG 546

Query: 554 NNGKFMDRSASLDSSYDSDSAGKVNRSCAEIDDDLLKKKHGNVLKKIGVLWKKQHK 610
           NNGK  +RS SLD++Y+S    K    C E+DDD   KK+GN+LKKIGVLW+K  K
Sbjct: 547 NNGKLAERSLSLDNNYNS-VMNKYTPFCEELDDDFQTKKNGNMLKKIGVLWRKPQK 588

BLAST of Carg12303-RA vs. TrEMBL
Match: tr|A0A1S3TJZ9|A0A1S3TJZ9_VIGRR (interactor of constitutive active ROPs 3 isoform X2 OS=Vigna radiata var. radiata OX=3916 GN=LOC106756269 PE=4 SV=1)

HSP 1 Score: 355.5 bits (911), Expect = 2.3e-94
Identity = 243/604 (40.23%), Postives = 333/604 (55.13%), Query Frame = 0

Query: 14  RNSFSEPPQKVSPRVARKLRPASLECDSASSTRTNNRTIKERSPKVVDRKSPRSPASEKK 73
           R+  SE PQKVSPR  R+LRP +++ DS SS+   N++ KERSPKV DR+SPRSP  E+K
Sbjct: 7   RSGSSEVPQKVSPRAVRQLRPTTVDTDSVSSSSQANKSSKERSPKVTDRRSPRSPVVERK 66

Query: 74  RPSKISELESQVCQLQENLTKVKDQLCLSESCKKEVKQEAEEAKNQLEAMALKLQXXXXX 133
           RPSKISELESQ+ QLQ +L KV DQL LSES K++ +Q+A E+K QL A++ K +     
Sbjct: 67  RPSKISELESQISQLQNDLKKVMDQLVLSESSKRQAQQDALESKEQLLALSAKFEDSQKH 126

Query: 134 XXXXXXXXXXXXXXXXXXXXERDRAWQXXXXXXXXXHKLDYAALATAVNEIHQLKIHLEM 193
                               ERD A Q           ++ AALA+A+NEI  LK  LE+
Sbjct: 127 VLDLCAAGEGRVLELQKIIDERDMACQSELEASKKQLSVESAALASAMNEIQMLKTELEL 186

Query: 194 VAQSETKKIKLTDSENNELDSLRIHLAEALCVXXXXXXXXXXXXXXXXXXXXXXXXXXXX 253
           VA  E                     +EAL +                            
Sbjct: 187 VANCEXXXXXXXXXXXXXXXXXXXXXSEALSLVDTMKNQLRDYKESEAQAQALVNETMLQ 246

Query: 254 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKARISVLEELVSKLKTDVANPA------ 313
                                           KAR++ LE LVSKL+TD  +        
Sbjct: 247 HEAAKKSIEILRADVAKAVDAYNTIALELDQSKARVNSLEALVSKLQTDPISSKFEHQPE 306

Query: 314 --VENEKQGDHIDEHCCLKTEIEQLKSALEMAETKYHEEQIQNAVKIRTAYEQVEQIKTN 373
             +E E   D   +   LK+E+ +L+SA+E AETKY EE+IQ+ V+IR+AYE VEQIK+ 
Sbjct: 307 IWIEGEGSDDLEAQVISLKSEVGRLQSAVETAETKYEEEKIQSTVQIRSAYELVEQIKSE 366

Query: 374 MNSKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAISEENEQLHAKIQKNLSSQREYELE 433
              +                               I EENE L+ K+Q+++SS+ E+EL+
Sbjct: 367 TGKREGELEAELKRKKADIEELKANLMDKETELQGIVEENENLNTKLQESMSSKNEHELK 426

Query: 434 REIKELKDHLAVMKSNLIDKDRQFRSVLDENDTLKLDINKRIMIRAKAIDGTTEVEDTTT 493
           RE K L++ +A +KS+++DK+   +S+ +EN+ LK++INK       + +   EVE    
Sbjct: 427 RETKRLEECVAELKSDMMDKETTLQSISEENEMLKMEINKLSDGGNVSEEVAAEVEGAKA 486

Query: 494 VEQEALTRLGVTIEETEKSNKRAARVSEQLEAAQAANVEIETELRRLRVQSDQWRKAAEA 553
            E+EAL +LG+ +EE ++SNK+AARV+EQLEAAQAAN+E+E ELRRL+VQSDQWRKA EA
Sbjct: 487 AEREALMKLGIVMEEADRSNKKAARVAEQLEAAQAANLEMEAELRRLKVQSDQWRKAXEA 546

Query: 554 AAAMLSAGNNGKFMDRSASLDSSYDSDSAGKVNRSCAEIDDDLLKKKHGNVLKKIGVLWK 610
           AAAMLS+GNNGK  +R+ SLD++Y      K +    ++DDD  +KK+GN+LKK GVLWK
Sbjct: 547 AAAMLSSGNNGKLTERTVSLDNNY------KCSPYAEDMDDDFQRKKNGNMLKKFGVLWK 604

BLAST of Carg12303-RA vs. TrEMBL
Match: tr|A0A1S3TK17|A0A1S3TK17_VIGRR (interactor of constitutive active ROPs 3 isoform X1 OS=Vigna radiata var. radiata OX=3916 GN=LOC106756269 PE=4 SV=1)

HSP 1 Score: 355.5 bits (911), Expect = 2.3e-94
Identity = 243/604 (40.23%), Postives = 333/604 (55.13%), Query Frame = 0

Query: 14  RNSFSEPPQKVSPRVARKLRPASLECDSASSTRTNNRTIKERSPKVVDRKSPRSPASEKK 73
           R+  SE PQKVSPR  R+LRP +++ DS SS+   N++ KERSPKV DR+SPRSP  E+K
Sbjct: 12  RSGSSEVPQKVSPRAVRQLRPTTVDTDSVSSSSQANKSSKERSPKVTDRRSPRSPVVERK 71

Query: 74  RPSKISELESQVCQLQENLTKVKDQLCLSESCKKEVKQEAEEAKNQLEAMALKLQXXXXX 133
           RPSKISELESQ+ QLQ +L KV DQL LSES K++ +Q+A E+K QL A++ K +     
Sbjct: 72  RPSKISELESQISQLQNDLKKVMDQLVLSESSKRQAQQDALESKEQLLALSAKFEDSQKH 131

Query: 134 XXXXXXXXXXXXXXXXXXXXERDRAWQXXXXXXXXXHKLDYAALATAVNEIHQLKIHLEM 193
                               ERD A Q           ++ AALA+A+NEI  LK  LE+
Sbjct: 132 VLDLCAAGEGRVLELQKIIDERDMACQSELEASKKQLSVESAALASAMNEIQMLKTELEL 191

Query: 194 VAQSETKKIKLTDSENNELDSLRIHLAEALCVXXXXXXXXXXXXXXXXXXXXXXXXXXXX 253
           VA  E                     +EAL +                            
Sbjct: 192 VANCEXXXXXXXXXXXXXXXXXXXXXSEALSLVDTMKNQLRDYKESEAQAQALVNETMLQ 251

Query: 254 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKARISVLEELVSKLKTDVANPA------ 313
                                           KAR++ LE LVSKL+TD  +        
Sbjct: 252 HEAAKKSIEILRADVAKAVDAYNTIALELDQSKARVNSLEALVSKLQTDPISSKFEHQPE 311

Query: 314 --VENEKQGDHIDEHCCLKTEIEQLKSALEMAETKYHEEQIQNAVKIRTAYEQVEQIKTN 373
             +E E   D   +   LK+E+ +L+SA+E AETKY EE+IQ+ V+IR+AYE VEQIK+ 
Sbjct: 312 IWIEGEGSDDLEAQVISLKSEVGRLQSAVETAETKYEEEKIQSTVQIRSAYELVEQIKSE 371

Query: 374 MNSKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAISEENEQLHAKIQKNLSSQREYELE 433
              +                               I EENE L+ K+Q+++SS+ E+EL+
Sbjct: 372 TGKREGELEAELKRKKADIEELKANLMDKETELQGIVEENENLNTKLQESMSSKNEHELK 431

Query: 434 REIKELKDHLAVMKSNLIDKDRQFRSVLDENDTLKLDINKRIMIRAKAIDGTTEVEDTTT 493
           RE K L++ +A +KS+++DK+   +S+ +EN+ LK++INK       + +   EVE    
Sbjct: 432 RETKRLEECVAELKSDMMDKETTLQSISEENEMLKMEINKLSDGGNVSEEVAAEVEGAKA 491

Query: 494 VEQEALTRLGVTIEETEKSNKRAARVSEQLEAAQAANVEIETELRRLRVQSDQWRKAAEA 553
            E+EAL +LG+ +EE ++SNK+AARV+EQLEAAQAAN+E+E ELRRL+VQSDQWRKA EA
Sbjct: 492 AEREALMKLGIVMEEADRSNKKAARVAEQLEAAQAANLEMEAELRRLKVQSDQWRKAXEA 551

Query: 554 AAAMLSAGNNGKFMDRSASLDSSYDSDSAGKVNRSCAEIDDDLLKKKHGNVLKKIGVLWK 610
           AAAMLS+GNNGK  +R+ SLD++Y      K +    ++DDD  +KK+GN+LKK GVLWK
Sbjct: 552 AAAMLSSGNNGKLTERTVSLDNNY------KCSPYAEDMDDDFQRKKNGNMLKKFGVLWK 609

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022949428.13.2e-22888.61interactor of constitutive active ROPs 3-like isoform X1 [Cucurbita moschata] >X... [more]
XP_023523260.11.1e-21788.78interactor of constitutive active ROPs 3-like [Cucurbita pepo subsp. pepo] >XP_0... [more]
XP_023521523.18.1e-21687.77interactor of constitutive active ROPs 3-like isoform X1 [Cucurbita pepo subsp. ... [more]
XP_022998865.19.0e-20772.03interactor of constitutive active ROPs 3-like isoform X1 [Cucurbita maxima][more]
XP_022949430.13.6e-17182.34interactor of constitutive active ROPs 3-like isoform X2 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT5G60210.11.3e-4235.24ROP interactive partner 5[more]
AT2G37080.11.7e-2926.88ROP interactive partner 3[more]
AT1G78430.16.8e-0740.37ROP interactive partner 2[more]
AT1G17140.11.4e-0437.27interactor of constitutive active rops 1[more]
Match NameE-valueIdentityDescription
sp|Q9LSS5|ICR3_ARATH2.4e-4135.24Interactor of constitutive active ROPs 3 OS=Arabidopsis thaliana OX=3702 GN=ICR3... [more]
sp|Q9ZQC5|ICR2_ARATH3.0e-2826.88Interactor of constitutive active ROPs 2, chloroplastic OS=Arabidopsis thaliana ... [more]
sp|Q9M9F9|ICR4_ARATH1.2e-0540.37Interactor of constitutive active ROPs 4 OS=Arabidopsis thaliana OX=3702 GN=ICR4... [more]
Match NameE-valueIdentityDescription
tr|A0A0A0LVB5|A0A0A0LVB5_CUCSA2.1e-15660.82Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G089500 PE=4 SV=1[more]
tr|A0A1S3CJE4|A0A1S3CJE4_CUCME2.7e-15161.97interactor of constitutive active ROPs 3-like OS=Cucumis melo OX=3656 GN=LOC1035... [more]
tr|A0A151SUZ5|A0A151SUZ5_CAJCA5.6e-9642.28Interactor of constitutive active ROPs 3 OS=Cajanus cajan OX=3821 GN=KK1_014035 ... [more]
tr|A0A1S3TJZ9|A0A1S3TJZ9_VIGRR2.3e-9440.23interactor of constitutive active ROPs 3 isoform X2 OS=Vigna radiata var. radiat... [more]
tr|A0A1S3TK17|A0A1S3TK17_VIGRR2.3e-9440.23interactor of constitutive active ROPs 3 isoform X1 OS=Vigna radiata var. radiat... [more]
The following terms have been associated with this mRNA:
Vocabulary: INTERPRO
TermDefinition
IPR029688ICR
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0003674 molecular_function

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Carg12303Carg12303gene


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg12303-RA.three_prime_UTR.1Carg12303-RA.three_prime_UTR.1three_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg12303-RA.CDS.2Carg12303-RA.CDS.2CDS
Carg12303-RA.CDS.1Carg12303-RA.CDS.1CDS


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg12303-RA.exon.2Carg12303-RA.exon.2exon
Carg12303-RA.exon.1Carg12303-RA.exon.1exon


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Carg12303-RACarg12303-RA-proteinpolypeptide


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 282..302
NoneNo IPR availableCOILSCoilCoilcoord: 212..239
NoneNo IPR availableCOILSCoilCoilcoord: 327..347
NoneNo IPR availableCOILSCoilCoilcoord: 498..535
NoneNo IPR availableCOILSCoilCoilcoord: 106..133
NoneNo IPR availableCOILSCoilCoilcoord: 244..267
NoneNo IPR availableCOILSCoilCoilcoord: 352..411
NoneNo IPR availableCOILSCoilCoilcoord: 421..441
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 51..79
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..22
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..79
NoneNo IPR availablePANTHERPTHR34224:SF5INTERACTOR OF CONSTITUTIVE ACTIVE ROPS 3coord: 13..609
IPR029688Interactor of constitutive active ROPsPANTHERPTHR34224FAMILY NOT NAMEDcoord: 13..609