Carg12171-RA (mRNA) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg12171-RA
Type	mRNA
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	Bile acid:sodium symporter
Location	Cucurbita_argyrosperma_scaffold_054 : 309378 .. 310621 (+)
Sequence length	749

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

CAATCTTCTTTCCATATTGGGCAAGAATTCATTTTCTAATTGAGCGCCACTCTACTTCGATTCAGCTACCATTCTGCAATTTCAACTGCAGCTACATGGCGTCATTTTCCAAATTCGAGTACACAGATTGTAGAACACACAAATTTGATGCTCTCTACAGGCAAACCTACGATTTCTCCGCGAAGAAGCCTACAGCCCATCTGTGTATGTTTTAGTTATCTACTTTAGGGTCACTCTCTATCTGGGCGCGCTGATTTTTGCTTCTATTTTCAAGTTTCATGAAGACACGGCTTAAATTTTTGCTTCTTTTTGTCTTTAGATATAAGGGGAAAATTATGTTCATCTCACAATGCAAGAAGTTTAGTAGCTCAGTGCAAACTGCTTAAGCCGATCGTTTTTTCTGGCTTTCCTTCTGCAATCATCTCAAGGTATGATGTTAAACATGTGACTATTGCTTCATATTGTCTAGAATTCCTTTCATGAAAGTTCATTCCCCCTTATATTTAGATTTACTTGTTCTTCCTGATGCTTATTGGTTACGTTCATAGATTTAGTTAGATTGGAGACTATACCTTATAATAAATACATATTGAACTATCAACTCAGGAAGCTCCAAATTCTGTGCAAAGCTGCAAATGATGTCTCTGGAGATATCCCTGATGGTAATTCTCCAAGTGGAATGAGTCTGTACGAAAGAACTATTGAAACTTTAACCACACTTTTTCCTCTGTGGGTAATGCTTATTAGTTCTGTTTTTGTGAATCTGGATTTAGTGCATGGTTGTGTTCTGGAGCTTTGCCTTTTAATTCCTGAGTTTGCCACCTATGAACAGGTTATCCTTGGAACCATTATCGGCATCTACAAACCTGCTGCAGTAATTGCTCATTCTTGGTTCTTCTTGATTGATGATTGGTGAATCTACTGGTACTCATCAGGCTCCCCTTGCAGGTAACTTGGTTGGAGACTGATCTTTTTACCCTGGGATTGGGTTTTCTTATGCTTTCGATGGGATTGACATTGACATTTGAGGATTTCAGGCGTTGTTTGAGAAATCCATGGACTGTAAGAGACATCCTTCTACTAACCAAATGGATGTACTTAAATAGTTGTGTTATTTAGCATCTATTTTCCTGGTGCAGGTTGGTGTAGGATTTCTTGCCCAGTACTTAATTAAACCTCTGCTTGGCTTTACTATCTCCATGGTAATAACTATGATGAAATCTTATAGCTCAATATCATGCTGA

mRNA sequence

CAATCTTCTTTCCATATTGGGCAAGAATTCATTTTCTAATTGAGCGCCACTCTACTTCGATTCAGCTACCATTCTGCAATTTCAACTGCAGCTACATGGCGTCATTTTCCAAATTCGAGTACACAGATTGTAGAACACACAAATTTGATGCTCTCTACAGGCAAACCTACGATTTCTCCGCGAAGAAGCCTACAGCCCATCTGTATATAAGGGGAAAATTATGTTCATCTCACAATGCAAGAAGTTTAGTAGCTCAGTGCAAACTGCTTAAGCCGATCGTTTTTTCTGGCTTTCCTTCTGCAATCATCTCAAGGAAGCTCCAAATTCTGTGCAAAGCTGCAAATGATGTCTCTGGAGATATCCCTGATGGTAATTCTCCAAGTGGAATGAGTCTGTACGAAAGAACTATTGAAACTTTAACCACACTTTTTCCTCTGTGGGTTATCCTTGGAACCATTATCGGCATCTACAAACCTGCTGCAGTAACTTGGTTGGAGACTGATCTTTTTACCCTGGGATTGGGTTTTCTTATGCTTTCGATGGGATTGACATTGACATTTGAGGATTTCAGGCGTTGTTTGAGAAATCCATGGACTGTAAGAGACATCCTTCTACTAACCAAATGGATGTACTTAAATAGTTGTGTTGGTGTAGGATTTCTTGCCCAGTACTTAATTAAACCTCTGCTTGGCTTTACTATCTCCATGGTAATAACTATGATGAAATCTTATAGCTCAATATCATGCTGA

Coding sequence (CDS)

ATGGCGTCATTTTCCAAATTCGAGTACACAGATTGTAGAACACACAAATTTGATGCTCTCTACAGGCAAACCTACGATTTCTCCGCGAAGAAGCCTACAGCCCATCTGTATATAAGGGGAAAATTATGTTCATCTCACAATGCAAGAAGTTTAGTAGCTCAGTGCAAACTGCTTAAGCCGATCGTTTTTTCTGGCTTTCCTTCTGCAATCATCTCAAGGAAGCTCCAAATTCTGTGCAAAGCTGCAAATGATGTCTCTGGAGATATCCCTGATGGTAATTCTCCAAGTGGAATGAGTCTGTACGAAAGAACTATTGAAACTTTAACCACACTTTTTCCTCTGTGGGTTATCCTTGGAACCATTATCGGCATCTACAAACCTGCTGCAGTAACTTGGTTGGAGACTGATCTTTTTACCCTGGGATTGGGTTTTCTTATGCTTTCGATGGGATTGACATTGACATTTGAGGATTTCAGGCGTTGTTTGAGAAATCCATGGACTGTAAGAGACATCCTTCTACTAACCAAATGGATGTACTTAAATAGTTGTGTTGGTGTAGGATTTCTTGCCCAGTACTTAATTAAACCTCTGCTTGGCTTTACTATCTCCATGGTAATAACTATGATGAAATCTTATAGCTCAATATCATGCTGA

Protein sequence

MASFSKFEYTDCRTHKFDALYRQTYDFSAKKPTAHLYIRGKLCSSHNARSLVAQCKLLKPIVFSGFPSAIISRKLQILCKAANDVSGDIPDGNSPSGMSLYERTIETLTTLFPLWVILGTIIGIYKPAAVTWLETDLFTLGLGFLMLSMGLTLTFEDFRRCLRNPWTVRDILLLTKWMYLNSCVGVGFLAQYLIKPLLGFTISMVITMMKSYSSISC

Homology

[Close]

BLAST of Carg12171-RA vs. NCBI nr
Match: XP_022932828.1 (sodium/pyruvate cotransporter BASS2, chloroplastic [Cucurbita moschata])

HSP 1 Score: 360.1 bits (923), Expect = 5.1e-96
Identity = 183/208 (87.98%), Postives = 187/208 (89.90%), Query Frame = 0


Query: 1   MASFSKFEYTDCRTHKFDALYRQTYDFSAKKPTAHLYIRGKLCSSHNARSLVAQCKLLKP 60
           MASFSKFEYTDCRTH FDALYRQTYDFSAKKPTAHLYIRGKLCSSHNARSLVAQCKLLKP
Sbjct: 1   MASFSKFEYTDCRTHNFDALYRQTYDFSAKKPTAHLYIRGKLCSSHNARSLVAQCKLLKP 60

Query: 61  IVFSGFPSAIISRKLQILCKAANDVSGDIPDGNSPSGMSLYERTIETLTTLFPLWVILGT 120
           IVFSG PSAI SRKLQILCKAANDVSGDIP+GNSPSG+SLYERTIETLTTLFPLWVILGT
Sbjct: 61  IVFSGSPSAITSRKLQILCKAANDVSGDIPEGNSPSGVSLYERTIETLTTLFPLWVILGT 120

Query: 121 IIGIYKPAAVTWLETDLFTLGLGFLMLSMGLTLTFEDFRRCLRNPWTVRDILLLTKWMYL 180
           IIGIYKPAAVTWLETDLFTLGLGFLMLSMGLTLTFEDFRRCLRNPWT             
Sbjct: 121 IIGIYKPAAVTWLETDLFTLGLGFLMLSMGLTLTFEDFRRCLRNPWT------------- 180

Query: 181 NSCVGVGFLAQYLIKPLLGFTISMVITM 209
              VGVGFLAQYLIKPLLGFTISM + +
Sbjct: 181 ---VGVGFLAQYLIKPLLGFTISMTLKL 192

[Close]

BLAST of Carg12171-RA vs. NCBI nr
Match: XP_023531206.1 (sodium/pyruvate cotransporter BASS2, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 360.1 bits (923), Expect = 5.1e-96
Identity = 184/208 (88.46%), Postives = 186/208 (89.42%), Query Frame = 0


Query: 1   MASFSKFEYTDCRTHKFDALYRQTYDFSAKKPTAHLYIRGKLCSSHNARSLVAQCKLLKP 60
           MASFSKFEYTDCRTHKFDALYRQTYDFSAKKPTAHLYIRGK CSSHNARSLVAQCKLLKP
Sbjct: 1   MASFSKFEYTDCRTHKFDALYRQTYDFSAKKPTAHLYIRGKSCSSHNARSLVAQCKLLKP 60

Query: 61  IVFSGFPSAIISRKLQILCKAANDVSGDIPDGNSPSGMSLYERTIETLTTLFPLWVILGT 120
           IVFSG PSAI SRKLQILCKAANDVSGDIPD NSPSGMSLYERTIETLTTLFPLWVILGT
Sbjct: 61  IVFSGSPSAITSRKLQILCKAANDVSGDIPDDNSPSGMSLYERTIETLTTLFPLWVILGT 120

Query: 121 IIGIYKPAAVTWLETDLFTLGLGFLMLSMGLTLTFEDFRRCLRNPWTVRDILLLTKWMYL 180
           IIGIYKPAAVTWLETDLFTLGLGFLMLSMGLTLTFEDFRRCLRNPWT             
Sbjct: 121 IIGIYKPAAVTWLETDLFTLGLGFLMLSMGLTLTFEDFRRCLRNPWT------------- 180

Query: 181 NSCVGVGFLAQYLIKPLLGFTISMVITM 209
              VGVGFLAQYLIKPLLGFTISM + +
Sbjct: 181 ---VGVGFLAQYLIKPLLGFTISMTLKL 192

[Close]

BLAST of Carg12171-RA vs. NCBI nr
Match: XP_023001525.1 (sodium/pyruvate cotransporter BASS2, chloroplastic [Cucurbita maxima])

HSP 1 Score: 359.0 bits (920), Expect = 1.1e-95
Identity = 183/208 (87.98%), Postives = 186/208 (89.42%), Query Frame = 0


Query: 1   MASFSKFEYTDCRTHKFDALYRQTYDFSAKKPTAHLYIRGKLCSSHNARSLVAQCKLLKP 60
           MASFSKFEYTDCRTHKFDA YRQTYDFSAKKPT HLYIRGKLCSSHNARSLV+QCKLLKP
Sbjct: 1   MASFSKFEYTDCRTHKFDAPYRQTYDFSAKKPTDHLYIRGKLCSSHNARSLVSQCKLLKP 60

Query: 61  IVFSGFPSAIISRKLQILCKAANDVSGDIPDGNSPSGMSLYERTIETLTTLFPLWVILGT 120
           IVFSG PSAI SRKLQILCKAANDVSGDIPDGNSPSGMSLYERTIETLTTLFPLWVILGT
Sbjct: 61  IVFSGSPSAITSRKLQILCKAANDVSGDIPDGNSPSGMSLYERTIETLTTLFPLWVILGT 120

Query: 121 IIGIYKPAAVTWLETDLFTLGLGFLMLSMGLTLTFEDFRRCLRNPWTVRDILLLTKWMYL 180
           IIGIYKPAAVTWLETDLFTLGLGFLMLSMGLTLTFEDFRRCLRNPWT             
Sbjct: 121 IIGIYKPAAVTWLETDLFTLGLGFLMLSMGLTLTFEDFRRCLRNPWT------------- 180

Query: 181 NSCVGVGFLAQYLIKPLLGFTISMVITM 209
              VGVGFLAQYLIKPLLGFTISM + +
Sbjct: 181 ---VGVGFLAQYLIKPLLGFTISMTLKL 192

[Close]

BLAST of Carg12171-RA vs. NCBI nr
Match: XP_022145090.1 (sodium/pyruvate cotransporter BASS2, chloroplastic [Momordica charantia])

HSP 1 Score: 274.6 bits (701), Expect = 2.8e-70
Identity = 144/210 (68.57%), Postives = 164/210 (78.10%), Query Frame = 0


Query: 1   MASFSKFEYTDCRTHKFDALYRQTYDFSAKKPTAHLYIRGKLCSSHNARSLVAQCKLLKP 60
           MAS SKF + DCR  + DA+ R++Y  SA KP AHL ++GKLC S N RSLVAQ  LL+P
Sbjct: 29  MASISKFAFKDCRLQRNDAICRRSYSASAMKPQAHLDVKGKLCLSDNGRSLVAQFMLLRP 88

Query: 61  IVFSGFPSAI--ISRKLQILCKAANDVSGDIPDGNSPSGMSLYERTIETLTTLFPLWVIL 120
           ++ S  P A+   SRKLQ+LCKAAND SG++P GNSPSG SLYERTIETLTTLFPLWVIL
Sbjct: 89  VLSSASPCAVGQTSRKLQMLCKAANDASGNVP-GNSPSGTSLYERTIETLTTLFPLWVIL 148

Query: 121 GTIIGIYKPAAVTWLETDLFTLGLGFLMLSMGLTLTFEDFRRCLRNPWTVRDILLLTKWM 180
           GT+IGIY+PAAVTWLETDLFT+ LGFLMLSMGLTLTFEDFRRCLRNPWT           
Sbjct: 149 GTVIGIYRPAAVTWLETDLFTVALGFLMLSMGLTLTFEDFRRCLRNPWT----------- 208

Query: 181 YLNSCVGVGFLAQYLIKPLLGFTISMVITM 209
                VGVGFLAQYLIKP+LGFTISM + +
Sbjct: 209 -----VGVGFLAQYLIKPMLGFTISMTLKL 221

[Close]

BLAST of Carg12171-RA vs. NCBI nr
Match: XP_008463345.1 (PREDICTED: sodium/pyruvate cotransporter BASS2, chloroplastic [Cucumis melo])

HSP 1 Score: 262.3 bits (669), Expect = 1.4e-66
Identity = 142/212 (66.98%), Postives = 159/212 (75.00%), Query Frame = 0


Query: 1   MASFSKFEYTDCRTHKFDALYRQTYDFSAKKPTAHLYIRGKLCSSHNARS--LVAQCKLL 60
           MAS SKF + DC   +FDALY + + FSA K  +HL  RGKLC S + R   +VAQ  LL
Sbjct: 1   MASISKFAFNDCSIMRFDALYNRNFHFSAGKFQSHLDFRGKLCLSDSGRRRLVVAQSMLL 60

Query: 61  KPIVFSGFPSAI--ISRKLQILCKAANDVSGDIPDGNSPSGMSLYERTIETLTTLFPLWV 120
           KP+V S  P AI   SRKLQILCKAA+DVSG+I  G+SP GMS YER IETLTTLFP+WV
Sbjct: 61  KPLVSSASPCAIGVTSRKLQILCKAASDVSGEIVPGDSPDGMSTYERVIETLTTLFPVWV 120

Query: 121 ILGTIIGIYKPAAVTWLETDLFTLGLGFLMLSMGLTLTFEDFRRCLRNPWTVRDILLLTK 180
           ILGTIIGIYKPAAVTWLETDLFT+GLGFLMLSMGLTLTFEDFRRCLRNPWT         
Sbjct: 121 ILGTIIGIYKPAAVTWLETDLFTVGLGFLMLSMGLTLTFEDFRRCLRNPWT--------- 180

Query: 181 WMYLNSCVGVGFLAQYLIKPLLGFTISMVITM 209
                  VGVGFLAQYLIKP+LGF I+M + +
Sbjct: 181 -------VGVGFLAQYLIKPMLGFVIAMTLKL 196

[Close]

BLAST of Carg12171-RA vs. TAIR10
Match: AT2G26900.1 (Sodium Bile acid symporter family)

HSP 1 Score: 173.7 bits (439), Expect = 1.2e-43
Identity = 107/208 (51.44%), Postives = 133/208 (63.94%), Query Frame = 0


Query: 1   MASFSKFEYTDCRTHKFDALYRQTYDFSAKKPTAHLYIRGKLCSSHNARSLVAQCKLLKP 60
           MAS S+   TD R  +       ++  S  +   HL     +  S++  SL  Q    KP
Sbjct: 1   MASISRILPTDGRLSQ--CRINTSWVPSTTRTQTHLDFPKLVSVSNSGISLRIQNS--KP 60

Query: 61  IVFSGFPSAIISRKLQILCKAANDVSGDIPDGNSPSGMSLYERTIETLTTLFPLWVILGT 120
           I       A  SR+  ++CKAA  VSGD+P+ ++P  +S YE+ IE LTTLFPLWVILGT
Sbjct: 61  ISPVFALEATSSRR--VVCKAAAGVSGDLPE-STPKELSQYEKIIELLTTLFPLWVILGT 120

Query: 121 IIGIYKPAAVTWLETDLFTLGLGFLMLSMGLTLTFEDFRRCLRNPWTVRDILLLTKWMYL 180
           ++GI+KP+ VTWLETDLFTLGLGFLMLSMGLTLTFEDFRRCLRNPWT             
Sbjct: 121 LVGIFKPSLVTWLETDLFTLGLGFLMLSMGLTLTFEDFRRCLRNPWT------------- 180

Query: 181 NSCVGVGFLAQYLIKPLLGFTISMVITM 209
              VGVGFLAQY+IKP+LGF I+M + +
Sbjct: 181 ---VGVGFLAQYMIKPILGFLIAMTLKL 185

[Close]

BLAST of Carg12171-RA vs. TAIR10
Match: AT1G78560.1 (Sodium Bile acid symporter family)

HSP 1 Score: 66.2 bits (160), Expect = 2.8e-11
Identity = 40/129 (31.01%), Postives = 63/129 (48.84%), Query Frame = 0


Query: 86  SGDIPDGNSPSGMSLYERTIETLTTLFPLWVILGTIIGIYKPAAVTWLETDLFTLGLGFL 145
           S D+P     S     E   E ++T FP+WV LG ++G+ +P+   W+  +   +GL   
Sbjct: 75  SNDLPTEKKKSFGEWVEFVGEAVSTAFPIWVSLGCLLGLMRPSTFNWVTPNWTIVGLTIT 134

Query: 146 MLSMGLTLTFEDFRRCLRNPWTVRDILLLTKWMYLNSCVGVGFLAQYLIKPLLGFTISMV 205
           ML MG+TLT +D R  L  P          K ++       GFL QY + PL  F +S +
Sbjct: 135 MLGMGMTLTLDDLRGALSMP----------KELF------AGFLLQYSVMPLSAFFVSKL 187

Query: 206 ITMMKSYSS 215
           + +   Y++
Sbjct: 195 LNLPPHYAA 187

[Close]

BLAST of Carg12171-RA vs. TAIR10
Match: AT3G25410.1 (Sodium Bile acid symporter family)

HSP 1 Score: 53.5 bits (127), Expect = 1.9e-07
Identity = 31/104 (29.81%), Postives = 52/104 (50.00%), Query Frame = 0


Query: 109 TTLFPLWVILGTIIGIYKPAAVTWLETDLFTLGLGFLMLSMGLTLTFEDFRRCLRNPWTV 168
           + L P  V L  +  +  P + TW+  DL+   LG +MLS+G+ L+ +DF    + P   
Sbjct: 112 SALLPFVVALTAVAALSYPPSFTWVSKDLYAPALGGIMLSIGIQLSVDDFALAFKRPVP- 171

Query: 169 RDILLLTKWMYLNSCVGVGFLAQYLIKPLLGFTISMVITMMKSY 213
                          + VGF+AQY++KPLLG  ++    M +++
Sbjct: 172 ---------------LSVGFVAQYVLKPLLGVLVANAFGMPRTF 199

[Close]

BLAST of Carg12171-RA vs. Swiss-Prot
Match: sp|Q1EBV7|BASS2_ARATH (Sodium/pyruvate cotransporter BASS2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BASS2 PE=1 SV=1)

HSP 1 Score: 173.7 bits (439), Expect = 2.2e-42
Identity = 107/208 (51.44%), Postives = 133/208 (63.94%), Query Frame = 0


Query: 1   MASFSKFEYTDCRTHKFDALYRQTYDFSAKKPTAHLYIRGKLCSSHNARSLVAQCKLLKP 60
           MAS S+   TD R  +       ++  S  +   HL     +  S++  SL  Q    KP
Sbjct: 1   MASISRILPTDGRLSQ--CRINTSWVPSTTRTQTHLDFPKLVSVSNSGISLRIQNS--KP 60

Query: 61  IVFSGFPSAIISRKLQILCKAANDVSGDIPDGNSPSGMSLYERTIETLTTLFPLWVILGT 120
           I       A  SR+  ++CKAA  VSGD+P+ ++P  +S YE+ IE LTTLFPLWVILGT
Sbjct: 61  ISPVFALEATSSRR--VVCKAAAGVSGDLPE-STPKELSQYEKIIELLTTLFPLWVILGT 120

Query: 121 IIGIYKPAAVTWLETDLFTLGLGFLMLSMGLTLTFEDFRRCLRNPWTVRDILLLTKWMYL 180
           ++GI+KP+ VTWLETDLFTLGLGFLMLSMGLTLTFEDFRRCLRNPWT             
Sbjct: 121 LVGIFKPSLVTWLETDLFTLGLGFLMLSMGLTLTFEDFRRCLRNPWT------------- 180

Query: 181 NSCVGVGFLAQYLIKPLLGFTISMVITM 209
              VGVGFLAQY+IKP+LGF I+M + +
Sbjct: 181 ---VGVGFLAQYMIKPILGFLIAMTLKL 185

[Close]

BLAST of Carg12171-RA vs. Swiss-Prot
Match: sp|Q5VRB2|BASS2_ORYSJ (Probable sodium/metabolite cotransporter BASS2, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=BASS2 PE=2 SV=1)

HSP 1 Score: 170.6 bits (431), Expect = 1.9e-41
Identity = 86/136 (63.24%), Postives = 104/136 (76.47%), Query Frame = 0


Query: 73  RKLQILCKAANDVSGDIPDGNSPSGMSLYERTIETLTTLFPLWVILGTIIGIYKPAAVTW 132
           R  Q+LCKA  ++S ++P+ + PS  + YE+ +E LTTLFP+WVILGTIIGIYKP+ VTW
Sbjct: 76  RSCQLLCKAEANISSNLPE-SIPSEANQYEKIVELLTTLFPVWVILGTIIGIYKPSMVTW 135

Query: 133 LETDLFTLGLGFLMLSMGLTLTFEDFRRCLRNPWTVRDILLLTKWMYLNSCVGVGFLAQY 192
           LETDLFT+GLGFLMLSMGLTLTFEDFRRC+RNPWT                VGVGFLAQY
Sbjct: 136 LETDLFTVGLGFLMLSMGLTLTFEDFRRCMRNPWT----------------VGVGFLAQY 194

Query: 193 LIKPLLGFTISMVITM 209
           LIKP+LGF I+M + +
Sbjct: 196 LIKPMLGFAIAMTLKL 194

[Close]

BLAST of Carg12171-RA vs. Swiss-Prot
Match: sp|Q93YR2|BASS1_ARATH (Probable sodium/metabolite cotransporter BASS1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BASS1 PE=2 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 5.0e-10
Identity = 40/129 (31.01%), Postives = 63/129 (48.84%), Query Frame = 0


Query: 86  SGDIPDGNSPSGMSLYERTIETLTTLFPLWVILGTIIGIYKPAAVTWLETDLFTLGLGFL 145
           S D+P     S     E   E ++T FP+WV LG ++G+ +P+   W+  +   +GL   
Sbjct: 75  SNDLPTEKKKSFGEWVEFVGEAVSTAFPIWVSLGCLLGLMRPSTFNWVTPNWTIVGLTIT 134

Query: 146 MLSMGLTLTFEDFRRCLRNPWTVRDILLLTKWMYLNSCVGVGFLAQYLIKPLLGFTISMV 205
           ML MG+TLT +D R  L  P          K ++       GFL QY + PL  F +S +
Sbjct: 135 MLGMGMTLTLDDLRGALSMP----------KELF------AGFLLQYSVMPLSAFFVSKL 187

Query: 206 ITMMKSYSS 215
           + +   Y++
Sbjct: 195 LNLPPHYAA 187

[Close]

BLAST of Carg12171-RA vs. Swiss-Prot
Match: sp|Q7XVB3|BASS1_ORYSJ (Probable sodium/metabolite cotransporter BASS1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=BASS1 PE=2 SV=2)

HSP 1 Score: 58.5 bits (140), Expect = 1.0e-07
Identity = 42/148 (28.38%), Postives = 65/148 (43.92%), Query Frame = 0


Query: 81  AANDVSGDIPDGNSPS-------GMSLYERTIETLTTLFPLWVILGTIIGIYKPAAVTWL 140
           AA+     IP G S +          L  +  E L+  FP+WV     + +++P A  W+
Sbjct: 68  AADPAPSKIPGGGSGALEAGVVGWRDLLLQVGEVLSLGFPVWVASACAVALWRPPAFLWV 127

Query: 141 ETDLFTLGLGFLMLSMGLTLTFEDFRRCLRNPWTVRDILLLTKWMYLNSCVGVGFLAQYL 200
                 +G+ F ML MG+TLT +D +  L  P  +                  GFL QY 
Sbjct: 128 SPMAQIVGISFTMLGMGMTLTLDDLKTALLMPKELAS----------------GFLLQYS 187

Query: 201 IKPLLGFTISMVITMMKSYSS----ISC 218
           + PL GF IS ++ +   Y++    +SC
Sbjct: 188 VMPLSGFLISKLLNLPSYYAAGLILVSC 199

[Close]

BLAST of Carg12171-RA vs. Swiss-Prot
Match: sp|O34524|YOCS_BACSU (Uncharacterized sodium-dependent transporter YocS OS=Bacillus subtilis (strain 168) OX=224308 GN=yocS PE=3 SV=1)

HSP 1 Score: 55.8 bits (133), Expect = 6.7e-07
Identity = 31/92 (33.70%), Postives = 48/92 (52.17%), Query Frame = 0


Query: 112 FPLWVILGTIIGIYKPAAVTWLETDLFTLGLGFLMLSMGLTLTFEDFRRCLRNPWTVRDI 171
           F +WVI+  ++G   P+  TW+ +   T+ LG +M  MGLTL  +DF+  +R PW V   
Sbjct: 15  FGIWVIVFAVLGFSFPSLFTWI-SSYITIFLGIIMFGMGLTLQADDFKELVRKPWQVI-- 74

Query: 172 LLLTKWMYLNSCVGVGFLAQYLIKPLLGFTIS 204
                         +G +AQY I PL+ F ++
Sbjct: 75  --------------IGVIAQYTIMPLVAFGLA 89

[Close]

BLAST of Carg12171-RA vs. TrEMBL
Match: tr|A0A1S3CKL3|A0A1S3CKL3_CUCME (sodium/pyruvate cotransporter BASS2, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103501527 PE=4 SV=1)

HSP 1 Score: 262.3 bits (669), Expect = 9.6e-67
Identity = 142/212 (66.98%), Postives = 159/212 (75.00%), Query Frame = 0


Query: 1   MASFSKFEYTDCRTHKFDALYRQTYDFSAKKPTAHLYIRGKLCSSHNARS--LVAQCKLL 60
           MAS SKF + DC   +FDALY + + FSA K  +HL  RGKLC S + R   +VAQ  LL
Sbjct: 1   MASISKFAFNDCSIMRFDALYNRNFHFSAGKFQSHLDFRGKLCLSDSGRRRLVVAQSMLL 60

Query: 61  KPIVFSGFPSAI--ISRKLQILCKAANDVSGDIPDGNSPSGMSLYERTIETLTTLFPLWV 120
           KP+V S  P AI   SRKLQILCKAA+DVSG+I  G+SP GMS YER IETLTTLFP+WV
Sbjct: 61  KPLVSSASPCAIGVTSRKLQILCKAASDVSGEIVPGDSPDGMSTYERVIETLTTLFPVWV 120

Query: 121 ILGTIIGIYKPAAVTWLETDLFTLGLGFLMLSMGLTLTFEDFRRCLRNPWTVRDILLLTK 180
           ILGTIIGIYKPAAVTWLETDLFT+GLGFLMLSMGLTLTFEDFRRCLRNPWT         
Sbjct: 121 ILGTIIGIYKPAAVTWLETDLFTVGLGFLMLSMGLTLTFEDFRRCLRNPWT--------- 180

Query: 181 WMYLNSCVGVGFLAQYLIKPLLGFTISMVITM 209
                  VGVGFLAQYLIKP+LGF I+M + +
Sbjct: 181 -------VGVGFLAQYLIKPMLGFVIAMTLKL 196

[Close]

BLAST of Carg12171-RA vs. TrEMBL
Match: tr|A0A0A0LZK6|A0A0A0LZK6_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G404660 PE=4 SV=1)

HSP 1 Score: 257.7 bits (657), Expect = 2.4e-65
Identity = 137/212 (64.62%), Postives = 159/212 (75.00%), Query Frame = 0


Query: 1   MASFSKFEYTDCRTHKFDALYRQTYDFSAKKPTAHLYIRGKLCSSHNARS--LVAQCKLL 60
           MAS SK  + DCR  +FDAL+ + + FS  K  +HL +RGKLC S + R   +VAQ  LL
Sbjct: 1   MASISKLAFNDCRMQRFDALHNRNFHFSPGKLQSHLDVRGKLCLSDSRRRSLVVAQSMLL 60

Query: 61  KPIVFSGFPSA--IISRKLQILCKAANDVSGDIPDGNSPSGMSLYERTIETLTTLFPLWV 120
           KP+V S  P A  + SRKLQILC+AA+DVSG+I  G+SP GMS YER IETLTTLFP+WV
Sbjct: 61  KPLVSSASPCAVRVTSRKLQILCEAASDVSGEIVPGDSPDGMSTYERVIETLTTLFPVWV 120

Query: 121 ILGTIIGIYKPAAVTWLETDLFTLGLGFLMLSMGLTLTFEDFRRCLRNPWTVRDILLLTK 180
           ILGTI+GIYKPAAVTWLETDLFT+GLGFLMLSMGLTLTFEDFRRCLRNPWT         
Sbjct: 121 ILGTILGIYKPAAVTWLETDLFTVGLGFLMLSMGLTLTFEDFRRCLRNPWT--------- 180

Query: 181 WMYLNSCVGVGFLAQYLIKPLLGFTISMVITM 209
                  VGVGFLAQYLIKP+LGF I+M + +
Sbjct: 181 -------VGVGFLAQYLIKPMLGFVIAMTLKL 196

[Close]

BLAST of Carg12171-RA vs. TrEMBL
Match: tr|A0A061GUV1|A0A061GUV1_THECC (Transmembrane domain protein isoform 1 OS=Theobroma cacao OX=3641 GN=TCM_037960 PE=4 SV=1)

HSP 1 Score: 222.6 bits (566), Expect = 8.4e-55
Identity = 121/212 (57.08%), Postives = 147/212 (69.34%), Query Frame = 0


Query: 1   MASFSKFEYTDCRTHKFDALYRQTYDF--SAKKPTAHLYIRGKLCSSHNARSLVAQCKLL 60
           MAS S+F   DC  H +DA++R+ +    + +   AHL +R  +    N +    + K  
Sbjct: 1   MASLSRFVIKDCNLHTYDAVHRRPWSLIPARRLQAAHLDVRAGISVPENGKGWAIERKPS 60

Query: 61  KPIVFSGFPSAII--SRKLQILCKAANDVSGDIPDGNSPSGMSLYERTIETLTTLFPLWV 120
            P+V    PS+II  SR LQ++CKAA +VSGDIP   +P+GM+ YER IE LTTLFP+WV
Sbjct: 61  SPLVSLAAPSSIIPASRNLQVMCKAATNVSGDIP---TPNGMNQYERIIEILTTLFPVWV 120

Query: 121 ILGTIIGIYKPAAVTWLETDLFTLGLGFLMLSMGLTLTFEDFRRCLRNPWTVRDILLLTK 180
           ILGTIIGIYKPAAVTWLETDLFT+GLGFLMLSMGLTLTFEDFRRCLRNPWT         
Sbjct: 121 ILGTIIGIYKPAAVTWLETDLFTVGLGFLMLSMGLTLTFEDFRRCLRNPWT--------- 180

Query: 181 WMYLNSCVGVGFLAQYLIKPLLGFTISMVITM 209
                  VGVGFLAQYLIKP+LGF I+M + +
Sbjct: 181 -------VGVGFLAQYLIKPMLGFAIAMALKL 193

[Close]

BLAST of Carg12171-RA vs. TrEMBL
Match: tr|A0A1R3GIQ7|A0A1R3GIQ7_9ROSI (Bile acid:sodium symporter OS=Corchorus olitorius OX=93759 GN=COLO4_34960 PE=4 SV=1)

HSP 1 Score: 222.2 bits (565), Expect = 1.1e-54
Identity = 125/212 (58.96%), Postives = 144/212 (67.92%), Query Frame = 0


Query: 1   MASFSKFEYTDCRTHKFDALYRQTYDF--SAKKPTAHLYIRGKLCSSHNARSLVAQCKLL 60
           MAS S+F    C  H  D +YR+      + +   AHL +R  +    N + L    K +
Sbjct: 1   MASLSRFVAKSCSLHTNDCVYRRPRPLIPAIRLQAAHLDVRVGISVPENGKGLAIHSKQI 60

Query: 61  KPIVFSGFPSAII--SRKLQILCKAANDVSGDIPDGNSPSGMSLYERTIETLTTLFPLWV 120
            P+V  G PS+II  SR +Q+ C AA +VSGDIP   +PSGMS YER IETLTTLFP+WV
Sbjct: 61  SPLVALGAPSSIIPASRNVQVKCSAAANVSGDIP---TPSGMSQYERIIETLTTLFPVWV 120

Query: 121 ILGTIIGIYKPAAVTWLETDLFTLGLGFLMLSMGLTLTFEDFRRCLRNPWTVRDILLLTK 180
           ILGTIIGIYKPAAVTWLETDLFTLGLGFLMLSMGLTLTFEDFRRCLRNPWT         
Sbjct: 121 ILGTIIGIYKPAAVTWLETDLFTLGLGFLMLSMGLTLTFEDFRRCLRNPWT--------- 180

Query: 181 WMYLNSCVGVGFLAQYLIKPLLGFTISMVITM 209
                  VGVGFLAQYLIKPLLGF I+M + +
Sbjct: 181 -------VGVGFLAQYLIKPLLGFAIAMTLKL 193

[Close]

BLAST of Carg12171-RA vs. TrEMBL
Match: tr|A0A2P6RJ81|A0A2P6RJ81_ROSCH (Putative Bile acid:sodium symporter/arsenical resistance protein Acr3 OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr2g0089661 PE=4 SV=1)

HSP 1 Score: 221.5 bits (563), Expect = 1.9e-54
Identity = 117/208 (56.25%), Postives = 144/208 (69.23%), Query Frame = 0


Query: 1   MASFSKFEYTDCRTHKFDALYRQTYDFSAKKPTAHLYIRGKLCSSHNARSLVAQCKLLKP 60
           MAS S+F   D + +  +A YRQ+   S+++   H+ +RG++      RS   Q K   P
Sbjct: 1   MASLSRFALKDLKLNTCEAYYRQSSSLSSRRLPTHVDVRGEISVLEKGRSCAIQSKTWSP 60

Query: 61  IVFSGFPSAIISRKLQILCKAANDVSGDIPDGNSPSGMSLYERTIETLTTLFPLWVILGT 120
           IV +G      SR  ++LCKAA + SGD+P   +P+GMS YER IETLTTLFP+WVILGT
Sbjct: 61  IVAAGSSVVQTSRNSKVLCKAAANASGDLP---NPTGMSQYERIIETLTTLFPVWVILGT 120

Query: 121 IIGIYKPAAVTWLETDLFTLGLGFLMLSMGLTLTFEDFRRCLRNPWTVRDILLLTKWMYL 180
           I+GIYKP+AVTWLETDLFTLGLGFLMLSMGLTLTFEDFRRCLRNPWT             
Sbjct: 121 IVGIYKPSAVTWLETDLFTLGLGFLMLSMGLTLTFEDFRRCLRNPWT------------- 180

Query: 181 NSCVGVGFLAQYLIKPLLGFTISMVITM 209
              VGVGFLAQY+IKPLLGF I++ + +
Sbjct: 181 ---VGVGFLAQYMIKPLLGFVIALTLKL 189

The following BLAST results are available for this feature:

BLAST of Carg12171-RA vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022932828.1	5.1e-96	87.98	sodium/pyruvate cotransporter BASS2, chloroplastic [Cucurbita moschata]	[more]
XP_023531206.1	5.1e-96	88.46	sodium/pyruvate cotransporter BASS2, chloroplastic [Cucurbita pepo subsp. pepo]	[more]
XP_023001525.1	1.1e-95	87.98	sodium/pyruvate cotransporter BASS2, chloroplastic [Cucurbita maxima]	[more]
XP_022145090.1	2.8e-70	68.57	sodium/pyruvate cotransporter BASS2, chloroplastic [Momordica charantia]	[more]
XP_008463345.1	1.4e-66	66.98	PREDICTED: sodium/pyruvate cotransporter BASS2, chloroplastic [Cucumis melo]	[more]

BLAST of Carg12171-RA vs. TAIR10


Match Name	E-value	Identity	Description
AT2G26900.1	1.2e-43	51.44	Sodium Bile acid symporter family	[more]
AT1G78560.1	2.8e-11	31.01	Sodium Bile acid symporter family	[more]
AT3G25410.1	1.9e-07	29.81	Sodium Bile acid symporter family	[more]

BLAST of Carg12171-RA vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q1EBV7\|BASS2_ARATH	2.2e-42	51.44	Sodium/pyruvate cotransporter BASS2, chloroplastic OS=Arabidopsis thaliana OX=37...	[more]
sp\|Q5VRB2\|BASS2_ORYSJ	1.9e-41	63.24	Probable sodium/metabolite cotransporter BASS2, chloroplastic OS=Oryza sativa su...	[more]
sp\|Q93YR2\|BASS1_ARATH	5.0e-10	31.01	Probable sodium/metabolite cotransporter BASS1, chloroplastic OS=Arabidopsis tha...	[more]
sp\|Q7XVB3\|BASS1_ORYSJ	1.0e-07	28.38	Probable sodium/metabolite cotransporter BASS1, chloroplastic OS=Oryza sativa su...	[more]
sp\|O34524\|YOCS_BACSU	6.7e-07	33.70	Uncharacterized sodium-dependent transporter YocS OS=Bacillus subtilis (strain 1...	[more]

BLAST of Carg12171-RA vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A1S3CKL3\|A0A1S3CKL3_CUCME	9.6e-67	66.98	sodium/pyruvate cotransporter BASS2, chloroplastic OS=Cucumis melo OX=3656 GN=LO...	[more]
tr\|A0A0A0LZK6\|A0A0A0LZK6_CUCSA	2.4e-65	64.62	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G404660 PE=4 SV=1	[more]
tr\|A0A061GUV1\|A0A061GUV1_THECC	8.4e-55	57.08	Transmembrane domain protein isoform 1 OS=Theobroma cacao OX=3641 GN=TCM_037960 ...	[more]
tr\|A0A1R3GIQ7\|A0A1R3GIQ7_9ROSI	1.1e-54	58.96	Bile acid:sodium symporter OS=Corchorus olitorius OX=93759 GN=COLO4_34960 PE=4 S...	[more]
tr\|A0A2P6RJ81\|A0A2P6RJ81_ROSCH	1.9e-54	56.25	Putative Bile acid:sodium symporter/arsenical resistance protein Acr3 OS=Rosa ch...	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **Cellular Component**
Term	Definition
GO:0016020	membrane

Vocabulary: **INTERPRO**
Term	Definition
IPR004710	Bilac:Na_transpt
IPR038770	Na+/solute_symporter_sf
IPR002657	BilAc:Na_symport/Acr3

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0016020	membrane
molecular_function	GO:0003674	molecular_function

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Carg12171	Carg12171	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Carg12171-RA	Carg12171-RA-protein	polypeptide

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg12171-RA.exon.1	Carg12171-RA.exon.1	exon
Carg12171-RA.exon.2	Carg12171-RA.exon.2	exon
Carg12171-RA.exon.3	Carg12171-RA.exon.3	exon
Carg12171-RA.exon.4	Carg12171-RA.exon.4	exon
Carg12171-RA.exon.5	Carg12171-RA.exon.5	exon
Carg12171-RA.exon.6	Carg12171-RA.exon.6	exon

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg12171-RA.five_prime_UTR.1	Carg12171-RA.five_prime_UTR.1	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg12171-RA.CDS.1	Carg12171-RA.CDS.1	CDS
Carg12171-RA.CDS.2	Carg12171-RA.CDS.2	CDS
Carg12171-RA.CDS.3	Carg12171-RA.CDS.3	CDS
Carg12171-RA.CDS.4	Carg12171-RA.CDS.4	CDS
Carg12171-RA.CDS.5	Carg12171-RA.CDS.5	CDS
Carg12171-RA.CDS.6	Carg12171-RA.CDS.6	CDS

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002657	Bile acid:sodium symporter/arsenical resistance protein Acr3	PFAM	PF01758	SBF	coord: 139..209 e-value: 5.1E-8 score: 32.7
IPR038770	Sodium/solute symporter superfamily	GENE3D	G3DSA:1.20.1530.20		coord: 95..214 e-value: 8.9E-18 score: 66.3
None	No IPR available	PANTHER	PTHR10361:SF35	SODIUM/PYRUVATE COTRANSPORTER BASS2, CHLOROPLASTIC	coord: 98..212
IPR004710	Bile acid:sodium symporter	PANTHER	PTHR10361	SODIUM-BILE ACID COTRANSPORTER	coord: 98..212