Carg12014-RA (mRNA) Silver-seed gourd

NameCarg12014-RA
TypemRNA
OrganismCucurbita argyrosperma (Silver-seed gourd)
Descriptionchaperone protein DnaJ-like
LocationCucurbita_argyrosperma_scaffold_037 : 469299 .. 471764 (+)
Sequence length1119
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTTTCTACCGTCAACTCGCCCTTCGGAATAGACCACACACCAATCGTTGAAGAAACCAACGCAACGGAGCCTCTTCTTCTTCTTCTTCTTCTTTCTCCGTTTTTGAAGTCCACGGCGCTGTTATGGTGATCTGAAAGTTGTTTTTTGCTCCTTTTGTTTGGTGTGGTGGTTGTGTTTCGAGATTCTGCGGCTTCTCTTCTGTCTGATTCCGATTTTTCGATGGATCGGGGAGGAGGATCCGACGGCGGAGCTTGCTACTACACCATCCTTGGGATTCGTAAGGACGCCTCCTTCTCCGATGTTCGTGCTGCCTACCGCAAGCTCGCTCTGGTACTGAATTCGGTTTTCGATTTAATTGTTTTGAAATTCGATTTTTCTAGGTTATTCTTTCTGTCTCAGATTTTTAGTCGGAATGAAATTTGGAGGAGTTTTGTTTTGTTCTGTTCTGTTCTGTTTCTTCCGATTTGAGAGCGAATTTGTGAAATCCGAACTGTTTCTGTTCTCGTTTCTTTTGCGGTATAAGAAATGGCATCCGGACCGGTGTACGCGGAATCCTGCGGTGGCCGGAGAAGCCAAGCGGCAGTTTCAATTAGTACAAGAAGCGTATTCAGGTGAGATCATCGATTCTCCGCCCCTAATCCCGTCAATTTTGTAAACGCCGTTGAATTCCCCGTCAATGTTCATAGGTTAGGACACTAACGCGCGTTGATTGCTCCATCTGCCTCCTCTCTCACAGTTCTCTCCGATCAGGCGAAGAGGTCCGTCTACGACGCCGGTCTATACGATCCAACGGAAGAAGACGATGAGGTTCGTATAGATTTTTTTTATTTTTATTAATTACTATTCATTATTATTCCAATTAGATATTTTTGGCATGGCATGATTATTTTCCCGGAAAAGACGACGTCGTTTTCATCATGCTCTATTTATACAACTTCGTTATTATTTTTTATTCACCATTTTCCTTTCGCTGTTGTGATTATTCATCATTTCTCATTGCGTACAAAAGCTGTCATCCAATGGCGTCCTCTAGAACTTCCCCTTTTGAGATTAAAAAAAAAAAAAAAAAAAAAAAGAGATTCCATCTATGTTCAAAATTAATTCATTGTCATGTAGAAAATAAGGTTTCTTATGCAACAAATATTTAAAAAGCTAGATTATAGAAATTTTGTTCTTTGGTTAGATGAAATTGGTTAATATTTTGTTAATAAAGTATTATTGGGTTTTAGAAAATGTCATTAATACCCATAAAACTTGAACAAAAAAAAAAAAAAAAAAAAGAAAAAAAAAAAAGCTATTTTATCTATAGACATATATTGTTTATAGACATGTTCATTTAATCTGATAACTTGTCCTTGAACGGGCGATGGAGTATGGAAAGATTTTTCCTACCCTGAAGATTATATTGCCATACTCGATCCCGCCCAAGATCAATGATGAATTTCGTGAGTCAGAAATTAAAATAGGAGGCTAGAATAGGGATAGGTAAGAGAAAGAATTCTAGTCGGGTAATTATCGATTAGAATTGAAATGAATTGGTTTGTGGTTGATCTTGTTTGATATTTATATATGTATTTTTAATACTCTTTTAATCCTCTTCTTTACTTTCTAAAAAAAATTATTTTAATTTTTATATATATATATATATATATGTAAATTTTTTATGGACCAAATATTACTGAATTTTTCGCAATTATTAAAAAAAAAAAGGAATTTTTTTTTTTGTTCTTTTTCTTTTTTGAGGAAAAATAATGGTCTCATTTCCGCTTGCGGTGCAGGAATTTTGCGACTTTATGCAAGAAATGATGACAATGATGAACAACGCCAAACCGGAGGTAATAACATCCTGACCGTCCAATTTCGAAGCCAATTAATTTCAGCCATGGATTGTTCGTCGTCCACAAATATCTGAAATGCCTTTTTGGTTTTTTGGTTTTTTTTTAATAGGGTGACAGCTTCGAGGATCTACAGAGGATGTTCATGGAAATGGTCGGTTCAGATGGCATGGGAGGGTTCCAAGTGAACGACATTCCAACGGCCTCAAAGAGGCCACGTCCTACTAACGGTTCCAGATCTAGTGCGCCCAAACGGAACTCCTCCCGCTGCTAAAATTTTACAGTGACGAAAATCTCCCTGGCTGATGTGGGTATATTGATTTGGGTTTGGGGGATTTGAAGGGTGTTTTAACCTTTTTCCTTTAATGTAATTTAGTGCAAGTTGGAGGGGCTTTTAAGTCTTTTCATTGCTAGGAAGTGTTTGGCTATTTTTTTTTTCTTTTATTAATAAATAAACTATTTGATCATCCCGCTTCTCTGATTGGCTTCCTCCATTTTCTACTTGGCAATGAAAATGGGCTTTCTTGATGGTTACAAGTTTTAATAGCTTTTTTTTAAAGCCCACATTTATAAAAATACAATTTTAAAGTGAAAAGTCCAAAATGGGCATTATGAGATAAGGTTAAGATT

mRNA sequence

TTTTTCTACCGTCAACTCGCCCTTCGGAATAGACCACACACCAATCGTTGAAGAAACCAACGCAACGGAGCCTCTTCTTCTTCTTCTTCTTCTTTCTCCGTTTTTGAAGTCCACGGCGCTGTTATGGTGATCTGAAAGTTGTTTTTTGCTCCTTTTGTTTGGTGTGGTGGTTGTGTTTCGAGATTCTGCGGCTTCTCTTCTGTCTGATTCCGATTTTTCGATGGATCGGGGAGGAGGATCCGACGGCGGAGCTTGCTACTACACCATCCTTGGGATTCGTAAGGACGCCTCCTTCTCCGATGTTCGTGCTGCCTACCGCAAGCTCGCTCTGAGCGAATTTGTGAAATCCGAACTGTTTCTGTTCTCGTTTCTTTTGCGGTATAAGAAATGGCATCCGGACCGGTGTACGCGGAATCCTGCGGTGGCCGGAGAAGCCAAGCGGCAGTTTCAATTAGTACAAGAAGCGTATTCAGTTCTCTCCGATCAGGCGAAGAGGTCCGTCTACGACGCCGGTCTATACGATCCAACGGAAGAAGACGATGAGGAATTTTGCGACTTTATGCAAGAAATGATGACAATGATGAACAACGCCAAACCGGAGGGTGACAGCTTCGAGGATCTACAGAGGATGTTCATGGAAATGGTCGGTTCAGATGGCATGGGAGGGTTCCAAGTGAACGACATTCCAACGGCCTCAAAGAGGCCACGTCCTACTAACGGTTCCAGATCTAGTGCGCCCAAACGGAACTCCTCCCGCTGCTAAAATTTTACAGTGACGAAAATCTCCCTGGCTGATGTGGGTATATTGATTTGGGTTTGGGGGATTTGAAGGGTGTTTTAACCTTTTTCCTTTAATGTAATTTAGTGCAAGTTGGAGGGGCTTTTAAGTCTTTTCATTGCTAGGAAGTGTTTGGCTATTTTTTTTTTCTTTTATTAATAAATAAACTATTTGATCATCCCGCTTCTCTGATTGGCTTCCTCCATTTTCTACTTGGCAATGAAAATGGGCTTTCTTGATGGTTACAAGTTTTAATAGCTTTTTTTTAAAGCCCACATTTATAAAAATACAATTTTAAAGTGAAAAGTCCAAAATGGGCATTATGAGATAAGGTTAAGATT

Coding sequence (CDS)

ATGGATCGGGGAGGAGGATCCGACGGCGGAGCTTGCTACTACACCATCCTTGGGATTCGTAAGGACGCCTCCTTCTCCGATGTTCGTGCTGCCTACCGCAAGCTCGCTCTGAGCGAATTTGTGAAATCCGAACTGTTTCTGTTCTCGTTTCTTTTGCGGTATAAGAAATGGCATCCGGACCGGTGTACGCGGAATCCTGCGGTGGCCGGAGAAGCCAAGCGGCAGTTTCAATTAGTACAAGAAGCGTATTCAGTTCTCTCCGATCAGGCGAAGAGGTCCGTCTACGACGCCGGTCTATACGATCCAACGGAAGAAGACGATGAGGAATTTTGCGACTTTATGCAAGAAATGATGACAATGATGAACAACGCCAAACCGGAGGGTGACAGCTTCGAGGATCTACAGAGGATGTTCATGGAAATGGTCGGTTCAGATGGCATGGGAGGGTTCCAAGTGAACGACATTCCAACGGCCTCAAAGAGGCCACGTCCTACTAACGGTTCCAGATCTAGTGCGCCCAAACGGAACTCCTCCCGCTGCTAA

Protein sequence

MDRGGGSDGGACYYTILGIRKDASFSDVRAAYRKLALSEFVKSELFLFSFLLRYKKWHPDRCTRNPAVAGEAKRQFQLVQEAYSVLSDQAKRSVYDAGLYDPTEEDDEEFCDFMQEMMTMMNNAKPEGDSFEDLQRMFMEMVGSDGMGGFQVNDIPTASKRPRPTNGSRSSAPKRNSSRC
BLAST of Carg12014-RA vs. NCBI nr
Match: XP_022946488.1 (dnaJ homolog subfamily B member 8-like [Cucurbita moschata])

HSP 1 Score: 312.8 bits (800), Expect = 7.8e-82
Identity = 160/180 (88.89%), Postives = 160/180 (88.89%), Query Frame = 0

Query: 1   MDRGGGSDGGACYYTILGIRKDASFSDVRAAYRKLALSEFVKSELFLFSFLLRYKKWHPD 60
           MDRGGGSDGGACYYTILGIRKDASFSDVR AYRKLAL                  KWHPD
Sbjct: 1   MDRGGGSDGGACYYTILGIRKDASFSDVRTAYRKLAL------------------KWHPD 60

Query: 61  RCTRNPAVAGEAKRQFQLVQEAYSVLSDQAKRSVYDAGLYDPTEEDDEEFCDFMQEMMTM 120
           RCTRNPAVAGEAKRQFQLVQEAYSVLSDQAKRSVYDAGLYDPTEEDDEEFCDFMQEMMTM
Sbjct: 61  RCTRNPAVAGEAKRQFQLVQEAYSVLSDQAKRSVYDAGLYDPTEEDDEEFCDFMQEMMTM 120

Query: 121 MNNAKPEGDSFEDLQRMFMEMVGSDGMGGFQVNDIPTASKRPRPTNGSRSSAPKRNSSRC 180
           MNNAKPEGDSFEDLQRMFMEMVGSDGMGGFQVND PTASKRPRPTNGSRSSAPKRNSSRC
Sbjct: 121 MNNAKPEGDSFEDLQRMFMEMVGSDGMGGFQVNDNPTASKRPRPTNGSRSSAPKRNSSRC 162

BLAST of Carg12014-RA vs. NCBI nr
Match: XP_022999047.1 (dnaJ homolog subfamily B member 8-like [Cucurbita maxima])

HSP 1 Score: 309.3 bits (791), Expect = 8.6e-81
Identity = 158/180 (87.78%), Postives = 159/180 (88.33%), Query Frame = 0

Query: 1   MDRGGGSDGGACYYTILGIRKDASFSDVRAAYRKLALSEFVKSELFLFSFLLRYKKWHPD 60
           MDRGGGSDGGACYYTILGIRKDASFSDVR AYRKLAL                  KWHPD
Sbjct: 1   MDRGGGSDGGACYYTILGIRKDASFSDVRTAYRKLAL------------------KWHPD 60

Query: 61  RCTRNPAVAGEAKRQFQLVQEAYSVLSDQAKRSVYDAGLYDPTEEDDEEFCDFMQEMMTM 120
           RCTRNPAVAGEAKRQFQLVQEAYSVLSDQAKRSVYDAGLYDPTEEDDEEFCDFMQEMMTM
Sbjct: 61  RCTRNPAVAGEAKRQFQLVQEAYSVLSDQAKRSVYDAGLYDPTEEDDEEFCDFMQEMMTM 120

Query: 121 MNNAKPEGDSFEDLQRMFMEMVGSDGMGGFQVNDIPTASKRPRPTNGSRSSAPKRNSSRC 180
           M NAKPEGDSFEDLQRMFMEMVGSDGMGGFQVND PTASKRPRPTNGSRSSAPKRNS+RC
Sbjct: 121 MTNAKPEGDSFEDLQRMFMEMVGSDGMGGFQVNDNPTASKRPRPTNGSRSSAPKRNSTRC 162

BLAST of Carg12014-RA vs. NCBI nr
Match: XP_023545333.1 (dnaJ homolog subfamily B member 8-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 307.0 bits (785), Expect = 4.3e-80
Identity = 157/180 (87.22%), Postives = 158/180 (87.78%), Query Frame = 0

Query: 1   MDRGGGSDGGACYYTILGIRKDASFSDVRAAYRKLALSEFVKSELFLFSFLLRYKKWHPD 60
           MDRGGGSDGGACYYTILGIRKDASFSDVR AYRKLAL                  KWHPD
Sbjct: 1   MDRGGGSDGGACYYTILGIRKDASFSDVRTAYRKLAL------------------KWHPD 60

Query: 61  RCTRNPAVAGEAKRQFQLVQEAYSVLSDQAKRSVYDAGLYDPTEEDDEEFCDFMQEMMTM 120
           RCTRNPAVAGEAKRQFQLVQEAYSVLSDQAKRSVYDAGLYDPTEEDDEEFCDFMQEMMTM
Sbjct: 61  RCTRNPAVAGEAKRQFQLVQEAYSVLSDQAKRSVYDAGLYDPTEEDDEEFCDFMQEMMTM 120

Query: 121 MNNAKPEGDSFEDLQRMFMEMVGSDGMGGFQVNDIPTASKRPRPTNGSRSSAPKRNSSRC 180
           MNN KPEGDSFEDLQRMFMEMVGSDGMG FQVND PTASKRPRPTNGSRSSAPKR+SSRC
Sbjct: 121 MNNTKPEGDSFEDLQRMFMEMVGSDGMGAFQVNDNPTASKRPRPTNGSRSSAPKRSSSRC 162

BLAST of Carg12014-RA vs. NCBI nr
Match: XP_008453848.1 (PREDICTED: dnaJ homolog subfamily B member 8 [Cucumis melo])

HSP 1 Score: 278.5 bits (711), Expect = 1.6e-71
Identity = 146/179 (81.56%), Postives = 151/179 (84.36%), Query Frame = 0

Query: 1   MDRGGGSDGGACYYTILGIRKDASFSDVRAAYRKLALSEFVKSELFLFSFLLRYKKWHPD 60
           MDRGGGSDGGACYYTILGIRKDAS SD+R AYRKLAL                  KWHPD
Sbjct: 1   MDRGGGSDGGACYYTILGIRKDASLSDIRTAYRKLAL------------------KWHPD 60

Query: 61  RCTRNPAVAGEAKRQFQLVQEAYSVLSDQAKRSVYDAGLYDPTEEDDEEFCDFMQEMMTM 120
           R  RNPAVAGEAKRQFQLVQEAYSVLSDQ KRSVYDAGLYDPTEEDDEEFCDFMQEM+TM
Sbjct: 61  RWMRNPAVAGEAKRQFQLVQEAYSVLSDQTKRSVYDAGLYDPTEEDDEEFCDFMQEMITM 120

Query: 121 MNNAKPEGDSFEDLQRMFMEMVGSDGMGGFQVNDIPTASKRPRPTNGSRSSAPKRNSSR 180
           MNN KPEGDSFEDLQRMFMEMVGSDG+G F +ND PTASKRPRP NGSRSSAPKR+SSR
Sbjct: 121 MNNVKPEGDSFEDLQRMFMEMVGSDGVGMFNMNDNPTASKRPRP-NGSRSSAPKRSSSR 160

BLAST of Carg12014-RA vs. NCBI nr
Match: XP_022156437.1 (uncharacterized protein LOC111023328 [Momordica charantia])

HSP 1 Score: 276.6 bits (706), Expect = 6.2e-71
Identity = 143/180 (79.44%), Postives = 150/180 (83.33%), Query Frame = 0

Query: 1   MDRGGGSDGGACYYTILGIRKDASFSDVRAAYRKLALSEFVKSELFLFSFLLRYKKWHPD 60
           MDRGGGSDGG+CYYTILGIRKDASFSD+R AYRKLAL                  KWHPD
Sbjct: 1   MDRGGGSDGGSCYYTILGIRKDASFSDIRTAYRKLAL------------------KWHPD 60

Query: 61  RCTRNPAVAGEAKRQFQLVQEAYSVLSDQAKRSVYDAGLYDPTEEDDEEFCDFMQEMMTM 120
           R TR+PAVAGEAKRQFQLVQEAYSVLSDQAKRSVYDAGLYDPTEEDDEEFCDFMQEM+TM
Sbjct: 61  RWTRDPAVAGEAKRQFQLVQEAYSVLSDQAKRSVYDAGLYDPTEEDDEEFCDFMQEMITM 120

Query: 121 MNNAKPEGDSFEDLQRMFMEMVGSDGMGGFQVNDIPTASKRPRPTNGSRSSAPKRNSSRC 180
           MN+ KPEGDSFEDLQRMFMEMVGSDGMGG    D PT +KRPRP NGSR SA KRN+SRC
Sbjct: 121 MNSVKPEGDSFEDLQRMFMEMVGSDGMGGLSATDNPTTTKRPRP-NGSRCSAAKRNASRC 161

BLAST of Carg12014-RA vs. TAIR10
Match: AT1G56300.1 (Chaperone DnaJ-domain superfamily protein)

HSP 1 Score: 151.0 bits (380), Expect = 7.1e-37
Identity = 89/172 (51.74%), Postives = 105/172 (61.05%), Query Frame = 0

Query: 4   GGGSDGGACYYTILGIRKDASFSDVRAAYRKLALSEFVKSELFLFSFLLRYKKWHPDRCT 63
           GGGS+  + YYTILGIRKDAS SD+R AYRKLA+                  KWHPDR  
Sbjct: 5   GGGSNVRSSYYTILGIRKDASVSDIRTAYRKLAM------------------KWHPDRYA 64

Query: 64  RNPAVAGEAKRQFQLVQEAYSVLSDQAKRSVYDAGLYDPTEEDDEEFCDFMQEMMTMMNN 123
           RNP VAGEAKR+FQ +QEAYSVL+D+ KRS+YD GLYDP E+                  
Sbjct: 65  RNPGVAGEAKRRFQQIQEAYSVLNDENKRSMYDVGLYDPHEDXXXXXXXXXXXXXXXXXX 124

Query: 124 AKPEGDSFEDLQRMFMEMVGSDGMGGFQVNDIPTASKRPRPTNGSRSSAPKR 176
               G+S EDLQRMF +MVG DG+  +  N+ P  +KRPR  N SRSSA  R
Sbjct: 125 XXXAGESLEDLQRMFTDMVGGDGV-SYDCNNNPKGNKRPR-VNISRSSAAMR 156

BLAST of Carg12014-RA vs. TAIR10
Match: AT3G14200.1 (Chaperone DnaJ-domain superfamily protein)

HSP 1 Score: 110.5 bits (275), Expect = 1.1e-24
Identity = 62/137 (45.26%), Postives = 84/137 (61.31%), Query Frame = 0

Query: 14  YTILGIRKDASFSDVRAAYRKLALSEFVKSELFLFSFLLRYKKWHPDRCTRNPAVAGEAK 73
           Y +LG++K+ S +++R+AY+KLAL                  +WHPDRC+    V  EAK
Sbjct: 14  YAVLGLKKECSKTELRSAYKKLAL------------------RWHPDRCSSMEFVE-EAK 73

Query: 74  RQFQLVQEAYSVLSDQAKRSVYDAGLYDPTEEDDEE-FCDFMQEMMTMMNNAKPE----G 133
           ++FQ +QEAYSVLSD  KR +YD G Y+  ++DD+    DF+ EM TMMN +KP     G
Sbjct: 74  KKFQAIQEAYSVLSDSNKRFLYDVGAYNTDDDDDQNGMGDFLNEMATMMNQSKPSDNNTG 131

Query: 134 DSFEDLQRMFMEMVGSD 146
           DSFE LQ +F EM   D
Sbjct: 134 DSFEQLQDLFNEMFQGD 131

BLAST of Carg12014-RA vs. TAIR10
Match: AT1G71000.1 (Chaperone DnaJ-domain superfamily protein)

HSP 1 Score: 84.0 bits (206), Expect = 1.1e-16
Identity = 51/130 (39.23%), Postives = 64/130 (49.23%), Query Frame = 0

Query: 13  YYTILGIRKDASFSDVRAAYRKLALSEFVKSELFLFSFLLRYKKWHPDRCTRNPAVAGEA 72
           YY ILG+  D+S   +R AY KLA                  K WHPDR T++P  +GEA
Sbjct: 9   YYEILGVAVDSSAEQIRRAYHKLA------------------KIWHPDRWTKDPFRSGEA 68

Query: 73  KRQFQLVQEAYSVLSDQAKRSVYDAGLYDPTEEDDEEFCDFMQEMMTMMNNAKPEGDSFE 132
           KR+FQ +QEAYSVLSD+ KRS YD GL                         + +  S E
Sbjct: 69  KRRFQQIQEAYSVLSDERKRSSYDVGLXXXXXXXXXXXXXXXXXXXXXQTKREEKQYSLE 120

Query: 133 DLQRMFMEMV 143
           +LQ M  +MV
Sbjct: 129 ELQTMVDDMV 120

BLAST of Carg12014-RA vs. TAIR10
Match: AT1G72416.3 (Chaperone DnaJ-domain superfamily protein)

HSP 1 Score: 81.3 bits (199), Expect = 6.9e-16
Identity = 47/133 (35.34%), Postives = 70/133 (52.63%), Query Frame = 0

Query: 14  YTILGIRKDASFSDVRAAYRKLALSEFVKSELFLFSFLLRYKKWHPDRCTRNPAVAGEAK 73
           Y +L +  + +  D+R +Y+ L L                  KWHPDR         EAK
Sbjct: 7   YAVLDLNNECTQGDLRLSYKNLVL------------------KWHPDRFLEE-IEKDEAK 66

Query: 74  RQFQLVQEAYSVLSDQAKRSVYDAGLYDPTEEDDEEFCDFMQEMMTMMNNAKPEGD-SFE 133
            +FQ +Q AYSVLSD  KR +YD G YD +++D+    DF+ EM+T+M   +  GD S E
Sbjct: 67  MKFQSIQRAYSVLSDSNKRLLYDVGAYD-SDDDETGMADFINEMVTLMAQTQSTGDESLE 119

Query: 134 DLQRMFMEMVGSD 146
           + + +F E++  D
Sbjct: 127 EFEELFQELLKDD 119

BLAST of Carg12014-RA vs. TAIR10
Match: AT4G28480.1 (DNAJ heat shock family protein)

HSP 1 Score: 58.9 bits (141), Expect = 3.7e-09
Identity = 33/87 (37.93%), Postives = 47/87 (54.02%), Query Frame = 0

Query: 10 GACYYTILGIRKDASFSDVRAAYRKLALSEFVKSELFLFSFLLRYKKWHPDRCTRNPAVA 69
          G  YY +L + + A+  D++ AYRKLA+                  KWHPD   +NP   
Sbjct: 2  GVDYYKVLQVDRSANDDDLKKAYRKLAM------------------KWHPD---KNPNNK 61

Query: 70 GEAKRQFQLVQEAYSVLSDQAKRSVYD 97
           +A+ +F+ + EAY VLSD  KR+VYD
Sbjct: 62 KDAEAKFKQISEAYDVLSDPQKRAVYD 67

BLAST of Carg12014-RA vs. Swiss-Prot
Match: sp|Q5F3Z5|DNJB6_CHICK (DnaJ homolog subfamily B member 6 OS=Gallus gallus OX=9031 GN=DNAJB6 PE=2 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 4.9e-11
Identity = 38/84 (45.24%), Postives = 48/84 (57.14%), Query Frame = 0

Query: 13 YYTILGIRKDASFSDVRAAYRKLALSEFVKSELFLFSFLLRYKKWHPDRCTRNPAVAGEA 72
          YY +LG++K AS  D++ AYRKLAL                  KWHPD   +NP    EA
Sbjct: 4  YYEVLGVQKHASAEDIKKAYRKLAL------------------KWHPD---KNPENKEEA 63

Query: 73 KRQFQLVQEAYSVLSDQAKRSVYD 97
          ++QF+ V EAY VLSD  KR +YD
Sbjct: 64 EQQFKQVAEAYEVLSDAKKRDIYD 66

BLAST of Carg12014-RA vs. Swiss-Prot
Match: sp|A6Q486|DNAJ_NITSB (Chaperone protein DnaJ OS=Nitratiruptor sp. (strain SB155-2) OX=387092 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 6.4e-11
Identity = 47/138 (34.06%), Postives = 69/138 (50.00%), Query Frame = 0

Query: 13  YYTILGIRKDASFSDVRAAYRKLALSEFVKSELFLFSFLLRYKKWHPDRCTRNPAVAGEA 72
           YY +L + ++ASF +++ AYRKLAL                  K+HPDR   NP    EA
Sbjct: 6   YYELLEVDRNASFEEIKKAYRKLAL------------------KYHPDRNPDNP----EA 65

Query: 73  KRQFQLVQEAYSVLSDQAKRSVYDAGLYDPTEEDDEEFCDFMQEMMTMMNNAKPEGDSFE 132
           + +F+L+ EAY VLSD+ KR++YD   Y     +++ F  F Q+             SF+
Sbjct: 66  EEKFKLINEAYQVLSDEEKRALYDQ--YGKAGLENQGFSGFNQK-------------SFD 106

Query: 133 DLQRMFMEMVGSDGMGGF 151
           D+   F  + G    GGF
Sbjct: 126 DIMDFFESVFGETFGGGF 106

BLAST of Carg12014-RA vs. Swiss-Prot
Match: sp|Q0III6|DNJB6_BOVIN (DnaJ homolog subfamily B member 6 OS=Bos taurus OX=9913 GN=DNAJB6 PE=2 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 1.1e-10
Identity = 37/84 (44.05%), Postives = 48/84 (57.14%), Query Frame = 0

Query: 13 YYTILGIRKDASFSDVRAAYRKLALSEFVKSELFLFSFLLRYKKWHPDRCTRNPAVAGEA 72
          YY +LG+++ AS  D++ AYRKLAL                  KWHPD   +NP    EA
Sbjct: 4  YYEVLGVQRHASAEDIKKAYRKLAL------------------KWHPD---KNPENKEEA 63

Query: 73 KRQFQLVQEAYSVLSDQAKRSVYD 97
          +R+F+ V EAY VLSD  KR +YD
Sbjct: 64 ERKFKQVAEAYEVLSDAKKRDIYD 66

BLAST of Carg12014-RA vs. Swiss-Prot
Match: sp|Q6P642|DNJB6_XENTR (DnaJ homolog subfamily B member 6 OS=Xenopus tropicalis OX=8364 GN=dnajb6 PE=2 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 1.1e-10
Identity = 37/84 (44.05%), Postives = 49/84 (58.33%), Query Frame = 0

Query: 13 YYTILGIRKDASFSDVRAAYRKLALSEFVKSELFLFSFLLRYKKWHPDRCTRNPAVAGEA 72
          YY +LG++++AS  D++ AYRKLAL                  KWHPD   +NP    EA
Sbjct: 4  YYDVLGVQRNASPEDIKKAYRKLAL------------------KWHPD---KNPDNKDEA 63

Query: 73 KRQFQLVQEAYSVLSDQAKRSVYD 97
          +R+F+ V EAY VLSD  KR +YD
Sbjct: 64 ERRFKEVAEAYEVLSDSKKRDIYD 66

BLAST of Carg12014-RA vs. Swiss-Prot
Match: sp|Q5F1R6|DJC21_HUMAN (DnaJ homolog subfamily C member 21 OS=Homo sapiens OX=9606 GN=DNAJC21 PE=1 SV=2)

HSP 1 Score: 67.8 bits (164), Expect = 1.4e-10
Identity = 51/157 (32.48%), Postives = 75/157 (47.77%), Query Frame = 0

Query: 12  CYYTILGIRKDASFSDVRAAYRKLALSEFVKSELFLFSFLLRYKKWHPDRCTRNPAVAGE 71
           C+Y  LG+R+DAS  +++ AYRKLAL                  KWHPD+   N   A E
Sbjct: 3   CHYEALGVRRDASEEELKKAYRKLAL------------------KWHPDKNLDN---AAE 62

Query: 72  AKRQFQLVQEAYSVLSDQAKRSVYD-------AGLYDPTEEDDEEFCDFMQEM-MTMMNN 131
           A  QF+L+Q AY VLSD  +R+ YD        G +D   +DD    D ++   +T  + 
Sbjct: 63  AAEQFKLIQAAYDVLSDPQERAWYDNHREALLKGGFDGEYQDDS--LDLLRYFTVTCYSG 122

Query: 132 AKPEGDSFEDLQRMFMEMVGSDGMGGF---QVNDIPT 158
              +   F  + R   EM+  + +      +V+D PT
Sbjct: 123 YGDDEKGFYTVYRNVFEMIAKEELESVLEEEVDDFPT 136

BLAST of Carg12014-RA vs. TrEMBL
Match: tr|A0A1S3BXC3|A0A1S3BXC3_CUCME (dnaJ homolog subfamily B member 8 OS=Cucumis melo OX=3656 GN=LOC103494448 PE=4 SV=1)

HSP 1 Score: 278.5 bits (711), Expect = 1.1e-71
Identity = 146/179 (81.56%), Postives = 151/179 (84.36%), Query Frame = 0

Query: 1   MDRGGGSDGGACYYTILGIRKDASFSDVRAAYRKLALSEFVKSELFLFSFLLRYKKWHPD 60
           MDRGGGSDGGACYYTILGIRKDAS SD+R AYRKLAL                  KWHPD
Sbjct: 1   MDRGGGSDGGACYYTILGIRKDASLSDIRTAYRKLAL------------------KWHPD 60

Query: 61  RCTRNPAVAGEAKRQFQLVQEAYSVLSDQAKRSVYDAGLYDPTEEDDEEFCDFMQEMMTM 120
           R  RNPAVAGEAKRQFQLVQEAYSVLSDQ KRSVYDAGLYDPTEEDDEEFCDFMQEM+TM
Sbjct: 61  RWMRNPAVAGEAKRQFQLVQEAYSVLSDQTKRSVYDAGLYDPTEEDDEEFCDFMQEMITM 120

Query: 121 MNNAKPEGDSFEDLQRMFMEMVGSDGMGGFQVNDIPTASKRPRPTNGSRSSAPKRNSSR 180
           MNN KPEGDSFEDLQRMFMEMVGSDG+G F +ND PTASKRPRP NGSRSSAPKR+SSR
Sbjct: 121 MNNVKPEGDSFEDLQRMFMEMVGSDGVGMFNMNDNPTASKRPRP-NGSRSSAPKRSSSR 160

BLAST of Carg12014-RA vs. TrEMBL
Match: tr|A0A0A0LGH9|A0A0A0LGH9_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G033330 PE=4 SV=1)

HSP 1 Score: 270.0 bits (689), Expect = 3.8e-69
Identity = 142/179 (79.33%), Postives = 148/179 (82.68%), Query Frame = 0

Query: 1   MDRGGGSDGGACYYTILGIRKDASFSDVRAAYRKLALSEFVKSELFLFSFLLRYKKWHPD 60
           MDRGGGSDGG  YY ILGIRKDAS SD+R AYRKLAL                  KWHPD
Sbjct: 1   MDRGGGSDGGTSYYAILGIRKDASLSDIRTAYRKLAL------------------KWHPD 60

Query: 61  RCTRNPAVAGEAKRQFQLVQEAYSVLSDQAKRSVYDAGLYDPTEEDDEEFCDFMQEMMTM 120
           R  RNPAVAGEAKRQFQLVQEAYSVLSDQ KRSVYDAGLYDPTEEDDEEFCDFMQEM+TM
Sbjct: 61  RWMRNPAVAGEAKRQFQLVQEAYSVLSDQTKRSVYDAGLYDPTEEDDEEFCDFMQEMITM 120

Query: 121 MNNAKPEGDSFEDLQRMFMEMVGSDGMGGFQVNDIPTASKRPRPTNGSRSSAPKRNSSR 180
           MNN KPEGDSFEDLQ+MFMEMVGSDG+G F +ND PTASKRPRP NGSRSSAPKR+SSR
Sbjct: 121 MNNVKPEGDSFEDLQKMFMEMVGSDGVGMFNMNDNPTASKRPRP-NGSRSSAPKRSSSR 160

BLAST of Carg12014-RA vs. TrEMBL
Match: tr|A0A1R3JPI4|A0A1R3JPI4_COCAP (Uncharacterized protein OS=Corchorus capsularis OX=210143 GN=CCACVL1_04814 PE=4 SV=1)

HSP 1 Score: 236.1 bits (601), Expect = 6.1e-59
Identity = 125/180 (69.44%), Postives = 139/180 (77.22%), Query Frame = 0

Query: 1   MDRGGGSDGGACYYTILGIRKDASFSDVRAAYRKLALSEFVKSELFLFSFLLRYKKWHPD 60
           MDR GGS GG+CYYT+LGIRKDASFSD+RAAYRKLAL                  KWHPD
Sbjct: 1   MDREGGSRGGSCYYTVLGIRKDASFSDIRAAYRKLAL------------------KWHPD 60

Query: 61  RCTRNPAVAGEAKRQFQLVQEAYSVLSDQAKRSVYDAGLYDPTEEDDEEFCDFMQEMMTM 120
           R  RNP VAGEAKR+FQ +QEAYSVLSD++KRS+YDAGLYDP EE+DEEFCDFMQEM++M
Sbjct: 61  RYARNPGVAGEAKRRFQQIQEAYSVLSDESKRSMYDAGLYDPLEEEDEEFCDFMQEMISM 120

Query: 121 MNNAKPEGDSFEDLQRMFMEMVGSDGMGGFQVNDIPTASKRPRPTNGSRSSAPKRNSSRC 180
           MNN K EGDSFEDLQRMF EMVG DGM  F VN  PT +KR R T  S+ +A KRNSSRC
Sbjct: 121 MNNVKDEGDSFEDLQRMFAEMVGGDGM-SFDVNTDPTETKRARVT-ASKGNAAKRNSSRC 160

BLAST of Carg12014-RA vs. TrEMBL
Match: tr|W9R8I6|W9R8I6_9ROSA (DnaJ homolog subfamily B member 6 OS=Morus notabilis OX=981085 GN=L484_002892 PE=4 SV=1)

HSP 1 Score: 229.2 bits (583), Expect = 7.5e-57
Identity = 124/183 (67.76%), Postives = 147/183 (80.33%), Query Frame = 0

Query: 1   MDRGGGSDGGACYYTILGIRKDASFSDVRAAYRKLALSEFVKSELFL-FSFLLRYKKWHP 60
           MDR GGS+GG+CYY +LGIRKD SF+DVR AYRKLAL ++ ++  F   SF     KWHP
Sbjct: 1   MDR-GGSNGGSCYYAVLGIRKDVSFADVRTAYRKLALVKYRQTTAFCSLSF-----KWHP 60

Query: 61  DRCTRNPAVAGEAKRQFQLVQEAYSVLSDQAKRSVYDAGLYDPTEEDDEEFCDFMQEMMT 120
           DR TRNPAVAGEAKR+FQ +QEAYSVLSDQ KRS+YDAGLY+P EE+DEEFCDFMQEM++
Sbjct: 61  DRWTRNPAVAGEAKRRFQQIQEAYSVLSDQTKRSMYDAGLYNPLEEEDEEFCDFMQEMIS 120

Query: 121 MMNNAKPEGDSFEDLQRMFMEMVGSDGMGGFQVNDIPTASKRPRPTNGSRSSAPKR--NS 180
           +MNN K EGDSFEDLQRMF+EMVG DG   F + + PTA+K+ R  N S+SSA KR  N+
Sbjct: 121 IMNNVKDEGDSFEDLQRMFVEMVGGDGT-SFDLKEDPTATKKAR-VNASKSSAAKRKCNA 175

BLAST of Carg12014-RA vs. TrEMBL
Match: tr|W9R342|W9R342_9ROSA (DnaJ homolog subfamily B member 6 OS=Morus notabilis OX=981085 GN=L484_006216 PE=4 SV=1)

HSP 1 Score: 228.8 bits (582), Expect = 9.7e-57
Identity = 124/183 (67.76%), Postives = 146/183 (79.78%), Query Frame = 0

Query: 1   MDRGGGSDGGACYYTILGIRKDASFSDVRAAYRKLALSEFVKSELFL-FSFLLRYKKWHP 60
           MDR GGS+GG+CYY +LGIRKD SF+DVR AYRKLAL ++ ++  F   SF     KWHP
Sbjct: 1   MDR-GGSNGGSCYYAVLGIRKDVSFADVRTAYRKLALVKYRQTTAFCSLSF-----KWHP 60

Query: 61  DRCTRNPAVAGEAKRQFQLVQEAYSVLSDQAKRSVYDAGLYDPTEEDDEEFCDFMQEMMT 120
           DR TRNPAVAGEAKR+FQ +QEAYSVLSDQ KRS+YDAGLY+P EE+DEEFCDFMQEM++
Sbjct: 61  DRWTRNPAVAGEAKRRFQQIQEAYSVLSDQTKRSMYDAGLYNPLEEEDEEFCDFMQEMIS 120

Query: 121 MMNNAKPEGDSFEDLQRMFMEMVGSDGMGGFQVNDIPTASKRPRPTNGSRSSAPKR--NS 180
           MMNN K EGDSFEDLQRMF+EMVG DG   F + + PTA+K+ R  N S+SSA K   N+
Sbjct: 121 MMNNVKDEGDSFEDLQRMFVEMVGGDGT-SFDLKEDPTATKKAR-VNASKSSAAKHKCNA 175

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022946488.17.8e-8288.89dnaJ homolog subfamily B member 8-like [Cucurbita moschata][more]
XP_022999047.18.6e-8187.78dnaJ homolog subfamily B member 8-like [Cucurbita maxima][more]
XP_023545333.14.3e-8087.22dnaJ homolog subfamily B member 8-like [Cucurbita pepo subsp. pepo][more]
XP_008453848.11.6e-7181.56PREDICTED: dnaJ homolog subfamily B member 8 [Cucumis melo][more]
XP_022156437.16.2e-7179.44uncharacterized protein LOC111023328 [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT1G56300.17.1e-3751.74Chaperone DnaJ-domain superfamily protein[more]
AT3G14200.11.1e-2445.26Chaperone DnaJ-domain superfamily protein[more]
AT1G71000.11.1e-1639.23Chaperone DnaJ-domain superfamily protein[more]
AT1G72416.36.9e-1635.34Chaperone DnaJ-domain superfamily protein[more]
AT4G28480.13.7e-0937.93DNAJ heat shock family protein[more]
Match NameE-valueIdentityDescription
sp|Q5F3Z5|DNJB6_CHICK4.9e-1145.24DnaJ homolog subfamily B member 6 OS=Gallus gallus OX=9031 GN=DNAJB6 PE=2 SV=1[more]
sp|A6Q486|DNAJ_NITSB6.4e-1134.06Chaperone protein DnaJ OS=Nitratiruptor sp. (strain SB155-2) OX=387092 GN=dnaJ P... [more]
sp|Q0III6|DNJB6_BOVIN1.1e-1044.05DnaJ homolog subfamily B member 6 OS=Bos taurus OX=9913 GN=DNAJB6 PE=2 SV=1[more]
sp|Q6P642|DNJB6_XENTR1.1e-1044.05DnaJ homolog subfamily B member 6 OS=Xenopus tropicalis OX=8364 GN=dnajb6 PE=2 S... [more]
sp|Q5F1R6|DJC21_HUMAN1.4e-1032.48DnaJ homolog subfamily C member 21 OS=Homo sapiens OX=9606 GN=DNAJC21 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
tr|A0A1S3BXC3|A0A1S3BXC3_CUCME1.1e-7181.56dnaJ homolog subfamily B member 8 OS=Cucumis melo OX=3656 GN=LOC103494448 PE=4 S... [more]
tr|A0A0A0LGH9|A0A0A0LGH9_CUCSA3.8e-6979.33Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G033330 PE=4 SV=1[more]
tr|A0A1R3JPI4|A0A1R3JPI4_COCAP6.1e-5969.44Uncharacterized protein OS=Corchorus capsularis OX=210143 GN=CCACVL1_04814 PE=4 ... [more]
tr|W9R8I6|W9R8I6_9ROSA7.5e-5767.76DnaJ homolog subfamily B member 6 OS=Morus notabilis OX=981085 GN=L484_002892 PE... [more]
tr|W9R342|W9R342_9ROSA9.7e-5767.76DnaJ homolog subfamily B member 6 OS=Morus notabilis OX=981085 GN=L484_006216 PE... [more]
The following terms have been associated with this mRNA:
Vocabulary: INTERPRO
TermDefinition
IPR018253DnaJ_domain_CS
IPR036869J_dom_sf
IPR001623DnaJ_domain
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0003674 molecular_function

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Carg12014Carg12014gene


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Carg12014-RACarg12014-RA-proteinpolypeptide


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg12014-RA.exon.1Carg12014-RA.exon.1exon
Carg12014-RA.exon.2Carg12014-RA.exon.2exon
Carg12014-RA.exon.3Carg12014-RA.exon.3exon
Carg12014-RA.exon.4Carg12014-RA.exon.4exon
Carg12014-RA.exon.5Carg12014-RA.exon.5exon


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg12014-RA.five_prime_UTR.1Carg12014-RA.five_prime_UTR.1five_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg12014-RA.CDS.1Carg12014-RA.CDS.1CDS
Carg12014-RA.CDS.2Carg12014-RA.CDS.2CDS
Carg12014-RA.CDS.3Carg12014-RA.CDS.3CDS
Carg12014-RA.CDS.4Carg12014-RA.CDS.4CDS
Carg12014-RA.CDS.5Carg12014-RA.CDS.5CDS


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg12014-RA.three_prime_UTR.1Carg12014-RA.three_prime_UTR.1three_prime_UTR


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001623DnaJ domainPRINTSPR00625JDOMAINcoord: 14..32
score: 40.01
coord: 50..65
score: 28.61
coord: 71..91
score: 45.09
IPR001623DnaJ domainSMARTSM00271dnaj_3coord: 11..91
e-value: 5.3E-14
score: 62.5
IPR001623DnaJ domainPFAMPF00226DnaJcoord: 54..96
e-value: 3.5E-11
score: 42.9
IPR001623DnaJ domainPROSITEPS50076DNAJ_2coord: 12..99
score: 17.74
IPR001623DnaJ domainCDDcd06257DnaJcoord: 12..88
e-value: 7.95858E-19
score: 75.6597
IPR036869Chaperone J-domain superfamilyGENE3DG3DSA:1.10.287.110coord: 6..121
e-value: 1.5E-22
score: 81.6
IPR036869Chaperone J-domain superfamilySUPERFAMILYSSF46565Chaperone J-domaincoord: 13..99
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 149..180
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 158..180
NoneNo IPR availablePANTHERPTHR44743:SF2SUBFAMILY NOT NAMEDcoord: 1..37
NoneNo IPR availablePANTHERPTHR44743FAMILY NOT NAMEDcoord: 56..174
NoneNo IPR availablePANTHERPTHR44743:SF2SUBFAMILY NOT NAMEDcoord: 56..174
NoneNo IPR availablePANTHERPTHR44743FAMILY NOT NAMEDcoord: 1..37
IPR018253DnaJ domain, conserved sitePROSITEPS00636DNAJ_1coord: 76..95