Carg11234-RA (mRNA) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg11234-RA
Type	mRNA
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	Sentrin-specific protease 8
Location	Cucurbita_argyrosperma_scaffold_013 : 793920 .. 794737 (+)
Sequence length	720

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATCTTAAATTCTTGATTTACAACGGCGTCGTGCTTCGAAGATCTGATGTACGTAGTTTAGAAGATTGTAACTATTTGAACGATGTCATCATCGATTTCTATTTTGCCTACCTGTTCGAGTCACATCAATCTGACGACATACTGTTACTACCGACGAGTATCTCTTTTTTGCTGGGCAACGCTCTTGACTGGGATACTTTCATGGACACAATGGAATCTCTGAACTTCCATGAGAAGAAACTCATATTCTTCACCGTCAACGAGAATTCCAATTTCTCCTCTTCTGGCGGATCTCATTGGAGCTTGCTTGTCTACTGTAGAAAGCGAAATTTGTTCATGCATTACGATAGCCTTCACATGATGAATAATGAATCTGCATTCGCTATCTACAAATTTGTTAATAGTTATATCAGTTATGATTCAGTTAAGTTCATGGAATGCCGAGCACCTCGACAAAGGAACGGATATGATTGTGGATTGTACGTGATGGAAATTGCTAGAGTGATTTACGAGCGCTATTTTAACTTTGATCAGTTTCAAAACGAAAATGAAATTTGTGGGTCGTTTAACGTGGTCCAAAGTGTGAATTGTAAACATGTTGAGTCCAATATGCGGTCTGAAATGTTGCAGCTTATCTTAGGATTGAATCAATAAACCGATCTTTTTTTCCAATTGCAGTAATTTTGTTTATTATCGATCTGCATTACGATAGCCTTCACATAATGAATAATGAATCTGCATTCACCATCCACGAATCTGTCAATAGTTATATCAGTTACGATTCAACTGAGTTCATAGAATGCCAAGCACCTTGA

mRNA sequence

ATGGATCTTAAATTCTTGATTTACAACGGCGTCGTGCTTCGAAGATCTGATGTACGTAGTTTAGAAGATTGTAACTATTTGAACGATGTCATCATCGATTTCTATTTTGCCTACCTGTTCGAGTCACATCAATCTGACGACATACTGTTACTACCGACGAGTATCTCTTTTTTGCTGGGCAACGCTCTTGACTGGGATACTTTCATGGACACAATGGAATCTCTGAACTTCCATGAGAAGAAACTCATATTCTTCACCGTCAACGAGAATTCCAATTTCTCCTCTTCTGGCGGATCTCATTGGAGCTTGCTTGTCTACTGTAGAAAGCGAAATTTGTTCATGCATTACGATAGCCTTCACATGATGAATAATGAATCTGCATTCGCTATCTACAAATTTGTTAATAGTTATATCAGTTATGATTCAGTTAAGTTCATGGAATGCCGAGCACCTCGACAAAGGAACGGATATGATTGTGGATTGTACGTGATGGAAATTGCTAGAGTGATTTACGAGCGCTATTTTAACTTTGATCAGTTTCAAAACGAAAATGAAATTTGTGGGTCGTTTAACGTGGTCCAAAGTGTGAATTGTAAACATGTTGAGTCCAATATGCGCCTTCACATAATGAATAATGAATCTGCATTCACCATCCACGAATCTGTCAATAGTTATATCAGTTACGATTCAACTGAGTTCATAGAATGCCAAGCACCTTGA

Coding sequence (CDS)

ATGGATCTTAAATTCTTGATTTACAACGGCGTCGTGCTTCGAAGATCTGATGTACGTAGTTTAGAAGATTGTAACTATTTGAACGATGTCATCATCGATTTCTATTTTGCCTACCTGTTCGAGTCACATCAATCTGACGACATACTGTTACTACCGACGAGTATCTCTTTTTTGCTGGGCAACGCTCTTGACTGGGATACTTTCATGGACACAATGGAATCTCTGAACTTCCATGAGAAGAAACTCATATTCTTCACCGTCAACGAGAATTCCAATTTCTCCTCTTCTGGCGGATCTCATTGGAGCTTGCTTGTCTACTGTAGAAAGCGAAATTTGTTCATGCATTACGATAGCCTTCACATGATGAATAATGAATCTGCATTCGCTATCTACAAATTTGTTAATAGTTATATCAGTTATGATTCAGTTAAGTTCATGGAATGCCGAGCACCTCGACAAAGGAACGGATATGATTGTGGATTGTACGTGATGGAAATTGCTAGAGTGATTTACGAGCGCTATTTTAACTTTGATCAGTTTCAAAACGAAAATGAAATTTGTGGGTCGTTTAACGTGGTCCAAAGTGTGAATTGTAAACATGTTGAGTCCAATATGCGCCTTCACATAATGAATAATGAATCTGCATTCACCATCCACGAATCTGTCAATAGTTATATCAGTTACGATTCAACTGAGTTCATAGAATGCCAAGCACCTTGA

Protein sequence

MDLKFLIYNGVVLRRSDVRSLEDCNYLNDVIIDFYFAYLFESHQSDDILLLPTSISFLLGNALDWDTFMDTMESLNFHEKKLIFFTVNENSNFSSSGGSHWSLLVYCRKRNLFMHYDSLHMMNNESAFAIYKFVNSYISYDSVKFMECRAPRQRNGYDCGLYVMEIARVIYERYFNFDQFQNENEICGSFNVVQSVNCKHVESNMRLHIMNNESAFTIHESVNSYISYDSTEFIECQAP

Homology

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BLAST of Carg11234-RA vs. NCBI nr
Match: XP_022988745.1 (NEDD8-specific protease 1-like [Cucurbita maxima])

HSP 1 Score: 394.4 bits (1012), Expect = 2.7e-106
Identity = 190/210 (90.48%), Postives = 200/210 (95.24%), Query Frame = 0


Query: 1   MDLKFLIYNGVVLRRSDVRSLEDCNYLNDVIIDFYFAYLFESHQSDDILLLPTSISFLLG 60
           MD KFL YNGVVLRRSDVRSLEDCNYLND IIDFYFAYLFES+QSDDILL+PTSISFLLG
Sbjct: 1   MDPKFLTYNGVVLRRSDVRSLEDCNYLNDAIIDFYFAYLFESYQSDDILLVPTSISFLLG 60

Query: 61  NALDWDTFMDTMESLNFHEKKLIFFTVNENSNFSSSGGSHWSLLVYCRKRNLFMHYDSLH 120
           NA DWDTF+ T+ESLNFHEKKLIFFTVN+NSNFSSSGGSHWSLLVYCRKRNLFMHYDSLH
Sbjct: 61  NAPDWDTFIATVESLNFHEKKLIFFTVNDNSNFSSSGGSHWSLLVYCRKRNLFMHYDSLH 120

Query: 121 MMNNESAFAIYKFVNSYISYDSVKFMECRAPRQRNGYDCGLYVMEIARVIYERYFNFDQF 180
           MMNNESAFAIY+ VNSYISYDS +FMECRAPRQRNGYDCGLYVMEIARVIYERYFNFDQF
Sbjct: 121 MMNNESAFAIYESVNSYISYDSAEFMECRAPRQRNGYDCGLYVMEIARVIYERYFNFDQF 180

Query: 181 QNENEICGSFNVVQSVNCKHVESNMRLHIM 211
           QNE+EICGSFNVVQ VNCKHVESNMR  ++
Sbjct: 181 QNEDEICGSFNVVQRVNCKHVESNMRSEML 210

[Close]

BLAST of Carg11234-RA vs. NCBI nr
Match: XP_022942008.1 (NEDD8-specific protease 1-like [Cucurbita moschata])

HSP 1 Score: 389.4 bits (999), Expect = 8.7e-105
Identity = 189/211 (89.57%), Postives = 198/211 (93.84%), Query Frame = 0


Query: 1   MDLKFLIYNGVVLRRSDVRSLEDCNYLNDVIIDFYFAYLFESHQSDDILLLPTSISFLLG 60
           MD KFL YNGVVLRRSDVRSLEDCNYLND IIDFYFAYLFES+QSDDILL+ TSISFLLG
Sbjct: 1   MDPKFLTYNGVVLRRSDVRSLEDCNYLNDAIIDFYFAYLFESYQSDDILLISTSISFLLG 60

Query: 61  NALDWDTFMDTMESLNFHEKKLIFFTVNENSNFSSSGGSHWSLLVYCRKRNLFMHYDSLH 120
           NA DWDTF+ T+ESLNFHEKKLIFFTVN+NSNFSSSGGSHWSLLVYCRKRNLFMHYDSLH
Sbjct: 61  NASDWDTFVATVESLNFHEKKLIFFTVNDNSNFSSSGGSHWSLLVYCRKRNLFMHYDSLH 120

Query: 121 MMNNESAFAIYKFVNSYISYDSVKFMECRAPRQRNGYDCGLYVMEIARVIYERYFNFDQF 180
           MMNNESAFAIY+ VNSYISYDS +FMECRAPRQRN YDCGLYVMEIARVIYE YFNFDQF
Sbjct: 121 MMNNESAFAIYESVNSYISYDSAEFMECRAPRQRNEYDCGLYVMEIARVIYEHYFNFDQF 180

Query: 181 QNENEICGSFNVVQSVNCKHVESNMRLHIMN 212
           QNE+EICGSFNVVQSVNCKHVESNMR   +N
Sbjct: 181 QNEDEICGSFNVVQSVNCKHVESNMRSESIN 211

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BLAST of Carg11234-RA vs. NCBI nr
Match: XP_023526001.1 (NEDD8-specific protease 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 309.7 bits (792), Expect = 8.7e-81
Identity = 149/160 (93.12%), Postives = 153/160 (95.62%), Query Frame = 0


Query: 1   MDLKFLIYNGVVLRRSDVRSLEDCNYLNDVIIDFYFAYLFESHQSDDILLLPTSISFLLG 60
           MD KFL YNGVVLRR DVRSLEDCNYLNDVIIDFYFAYLFESHQSDDILLLPTSISFLLG
Sbjct: 1   MDPKFLTYNGVVLRRFDVRSLEDCNYLNDVIIDFYFAYLFESHQSDDILLLPTSISFLLG 60

Query: 61  NALDWDTFMDTMESLNFHEKKLIFFTVNENSNFSSSGGSHWSLLVYCRKRNLFMHYDSLH 120
           NALDWDTFM TMESLNFHEKKLIFFTVN+NSNFSSSGGSHWSLLVYCRKRNLFMH+DSLH
Sbjct: 61  NALDWDTFMATMESLNFHEKKLIFFTVNDNSNFSSSGGSHWSLLVYCRKRNLFMHHDSLH 120

Query: 121 MMNNESAFAIYKFVNSYISYDSVKFMECRAPRQRNGYDCG 161
           MMNNESAF IY+ VNSYISYDS +FMECRAPRQRNGYDCG
Sbjct: 121 MMNNESAFTIYESVNSYISYDSAEFMECRAPRQRNGYDCG 160

[Close]

BLAST of Carg11234-RA vs. NCBI nr
Match: XP_022953908.1 (NEDD8-specific protease 1-like [Cucurbita moschata])

HSP 1 Score: 301.6 bits (771), Expect = 2.4e-78
Identity = 145/210 (69.05%), Postives = 171/210 (81.43%), Query Frame = 0


Query: 1   MDLKFLIYNGVVLRRSDVRSLEDCNYLNDVIIDFYFAYLFESHQSDDILLLPTSISFLLG 60
           M  KFL Y  VV++  D+ SLE  NYLND IIDFYFAYL  ++QS++IL +PTS+SFLLG
Sbjct: 1   MAAKFLTYGDVVIQGFDLHSLEGRNYLNDTIIDFYFAYLSATYQSNEILSIPTSVSFLLG 60

Query: 61  NALDWDTFMDTMESLNFHEKKLIFFTVNENSNFSSSGGSHWSLLVYCRKRNLFMHYDSLH 120
           N LDWD F+ T+ESLN HEKKLIFF VN+NSN SSSGGSHWSLLVYCRKRNLFMHYDSL 
Sbjct: 61  NTLDWDIFISTIESLNLHEKKLIFFVVNDNSNVSSSGGSHWSLLVYCRKRNLFMHYDSLS 120

Query: 121 MMNNESAFAIYKFVNSYISYDSVKFMECRAPRQRNGYDCGLYVMEIARVIYERYFNFDQF 180
            MN+E AF +Y+ V+ YI+YD  +FMECRAPRQ NGYDCGLYVME ARVIYE YFN DQF
Sbjct: 121 TMNSEPAFTLYESVSGYINYDPAEFMECRAPRQNNGYDCGLYVMETARVIYEHYFNVDQF 180

Query: 181 QNENEICGSFNVVQSVNCKHVESNMRLHIM 211
           ++++EI G  NV++SV  K+VESNMR  +M
Sbjct: 181 RSKDEISGPINVMRSVKSKYVESNMRSEMM 210

[Close]

BLAST of Carg11234-RA vs. NCBI nr
Match: XP_023549404.1 (NEDD8-specific protease 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 294.3 bits (752), Expect = 3.8e-76
Identity = 142/210 (67.62%), Postives = 169/210 (80.48%), Query Frame = 0


Query: 1   MDLKFLIYNGVVLRRSDVRSLEDCNYLNDVIIDFYFAYLFESHQSDDILLLPTSISFLLG 60
           M  KFL Y  VV++ SD+ SLE  NYLND IIDFYFAYL  ++QS++IL +PTS+SFLLG
Sbjct: 1   MAAKFLTYGDVVIQGSDLHSLEGRNYLNDTIIDFYFAYLSATYQSNEILSIPTSVSFLLG 60

Query: 61  NALDWDTFMDTMESLNFHEKKLIFFTVNENSNFSSSGGSHWSLLVYCRKRNLFMHYDSLH 120
           N LDWD F+ T+ESLN  EKKLIFF VN+NSN SSSGGSHWSLLVYCRKRNLFMHYDSL 
Sbjct: 61  NTLDWDIFISTIESLNLPEKKLIFFVVNDNSNVSSSGGSHWSLLVYCRKRNLFMHYDSLS 120

Query: 121 MMNNESAFAIYKFVNSYISYDSVKFMECRAPRQRNGYDCGLYVMEIARVIYERYFNFDQF 180
            MN+ESAF +Y+ VN YI+Y+  +FMECR PRQ NGYDCGLYVME ARVIYE Y N DQF
Sbjct: 121 TMNSESAFTLYESVNGYINYEPAEFMECRTPRQNNGYDCGLYVMETARVIYEHYCNVDQF 180

Query: 181 QNENEICGSFNVVQSVNCKHVESNMRLHIM 211
           ++++EI G  NV++SV  K+VE NMR  ++
Sbjct: 181 RSKDEISGPINVMRSVKSKYVEFNMRSEML 210

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BLAST of Carg11234-RA vs. TAIR10
Match: AT5G60190.1 (Cysteine proteinases superfamily protein)

HSP 1 Score: 143.7 bits (361), Expect = 1.5e-34
Identity = 78/187 (41.71%), Postives = 115/187 (61.50%), Query Frame = 0


Query: 2   DLKFLIYNGVVLRRSDVRSLEDCNYLNDVIIDFYFAYLFESHQSDDILLLPTSISFLLGN 61
           D K L Y  VVLRRSD+  L    +LND +I+FY ++L   H S  I L+P SI+F + N
Sbjct: 7   DDKILSYEDVVLRRSDLDILNGPIFLNDRVIEFYLSFLSTVHSSTTISLIPPSIAFWISN 66

Query: 62  ALDWDTFMDTMESLNFHEKKLIFFTVNENSNFS-SSGGSHWSLLVYCRKRNLFMHYDSLH 121
             D +   D M+ LN  +K L+   VN+NSN   + GG HWSLLVY ++ N F+H+DS  
Sbjct: 67  CPDTEYLKDFMKPLNLRDKDLLILPVNDNSNVEVAEGGLHWSLLVYYKEANTFVHHDSYM 126

Query: 122 MMNNESAFAIYKFVNSYISYDSVKFMECR-APRQRNGYDCGLYVMEIARVIYERYFNFDQ 181
            +N  SA  ++K V+ ++S     + EC   P+Q+NGYDCG++++  ARVI E +F+   
Sbjct: 127 GVNRWSAKQLFKAVSPFVSNGDASYKECTDTPQQKNGYDCGVFLLATARVICE-WFSSGG 186

Query: 182 FQNENEI 187
            +N +E+
Sbjct: 187 MKNRDEL 192

[Close]

BLAST of Carg11234-RA vs. TAIR10
Match: AT1G09730.1 (Cysteine proteinases superfamily protein)

HSP 1 Score: 46.6 bits (109), Expect = 2.5e-05
Identity = 50/220 (22.73%), Postives = 84/220 (38.18%), Query Frame = 0


Query: 9   NGVVLRRSDVRSLEDCNYLNDVIIDFYFAYLFESHQSDDILLLPTSISFLLGNALDWDTF 68
           + V + + DV  L+   ++ND IIDFY  YL    Q+++        SF      D D  
Sbjct: 424 DAVSICKRDVELLQPETFVNDTIIDFYINYLKNQIQTEEKHRFHFFNSFFFRKLADLDKD 483

Query: 69  MDTM--------------ESLNFHEKKLIFFTVNENSNFSSSGGSHWSLLVYCRKRNLFM 128
             ++                ++   K  IF  VN N         HWSL+V C    +  
Sbjct: 484 PSSIADGKAAFLRVRKWTRKVDMFGKDYIFVPVNYN--------LHWSLIVICHPGEVAN 543

Query: 129 HYD-----------SLHMMNNESAFA-IYKFVNSYI-----------------SYDSVKF 185
             D            LHM + + + A +   V +Y+                  + +++F
Sbjct: 544 RTDLDLDDSKKVPCILHMDSIKGSHAGLKNLVQTYLCEEWKERHKETSDDISSRFMNLRF 603

[Close]

BLAST of Carg11234-RA vs. Swiss-Prot
Match: sp|Q9LSS7|RUBP1_ARATH (NEDD8-specific protease 1 OS=Arabidopsis thaliana OX=3702 GN=NEDP1 PE=2 SV=1)

HSP 1 Score: 143.7 bits (361), Expect = 2.7e-33
Identity = 78/187 (41.71%), Postives = 115/187 (61.50%), Query Frame = 0


Query: 2   DLKFLIYNGVVLRRSDVRSLEDCNYLNDVIIDFYFAYLFESHQSDDILLLPTSISFLLGN 61
           D K L Y  VVLRRSD+  L    +LND +I+FY ++L   H S  I L+P SI+F + N
Sbjct: 7   DDKILSYEDVVLRRSDLDILNGPIFLNDRVIEFYLSFLSTVHSSTTISLIPPSIAFWISN 66

Query: 62  ALDWDTFMDTMESLNFHEKKLIFFTVNENSNFS-SSGGSHWSLLVYCRKRNLFMHYDSLH 121
             D +   D M+ LN  +K L+   VN+NSN   + GG HWSLLVY ++ N F+H+DS  
Sbjct: 67  CPDTEYLKDFMKPLNLRDKDLLILPVNDNSNVEVAEGGLHWSLLVYYKEANTFVHHDSYM 126

Query: 122 MMNNESAFAIYKFVNSYISYDSVKFMECR-APRQRNGYDCGLYVMEIARVIYERYFNFDQ 181
            +N  SA  ++K V+ ++S     + EC   P+Q+NGYDCG++++  ARVI E +F+   
Sbjct: 127 GVNRWSAKQLFKAVSPFVSNGDASYKECTDTPQQKNGYDCGVFLLATARVICE-WFSSGG 186

Query: 182 FQNENEI 187
            +N +E+
Sbjct: 187 MKNRDEL 192

[Close]

BLAST of Carg11234-RA vs. Swiss-Prot
Match: sp|Q5FVJ8|SENP8_RAT (Sentrin-specific protease 8 OS=Rattus norvegicus OX=10116 GN=Senp8 PE=2 SV=1)

HSP 1 Score: 102.4 bits (254), Expect = 6.9e-21
Identity = 62/170 (36.47%), Postives = 96/170 (56.47%), Query Frame = 0


Query: 1   MDLKFLIYNGVVLRRSDVRSLEDCNYLNDVIIDFYFAYLFESHQ----SDDILLLPTSIS 60
           MD   L Y   +LR+SDV  L+  N+LND II F F Y F S Q    SD +  +   ++
Sbjct: 1   MDPVVLSYMDSLLRQSDVSLLDPPNWLNDHIIGFAFEY-FASSQFHDCSDHVCFISPEVT 60

Query: 61  FLLGNALDWDTFMDTMESLNFHEKKLIFFTVNENSNFSSSGGSHWSLLVYCRKRNLFMHY 120
             +            +E L+   K+++F  +N+NSN  ++GG+HWSLLVY + +N F HY
Sbjct: 61  QFIKCTSSPAEIAMFLEPLDLPHKRVVFLAINDNSN-QAAGGTHWSLLVYLQDKNSFFHY 120

Query: 121 DSLHMMNNESAFAIYKFVNSYISY--DSVKFMECRAPRQRNGYDCGLYVM 165
           DS    N+  A  + + + +++    D + F+E +AP Q+N YDCG+YV+
Sbjct: 121 DSHSRSNSIHAKQVAEKLKAFLGSKGDKLVFVEEKAPAQQNSYDCGMYVI 168

[Close]

BLAST of Carg11234-RA vs. Swiss-Prot
Match: sp|Q96LD8|SENP8_HUMAN (Sentrin-specific protease 8 OS=Homo sapiens OX=9606 GN=SENP8 PE=1 SV=1)

HSP 1 Score: 102.1 bits (253), Expect = 9.0e-21
Identity = 60/180 (33.33%), Postives = 99/180 (55.00%), Query Frame = 0


Query: 1   MDLKFLIYNGVVLRRSDVRSLEDCNYLNDVIIDFYFAYLFESH---QSDDILLLPTSISF 60
           MD   L Y   +LR+SDV  L+  ++LND II F F Y   S     SD +  +   ++ 
Sbjct: 1   MDPVVLSYMDSLLRQSDVSLLDPPSWLNDHIIGFAFEYFANSQFHDCSDHVSFISPEVTQ 60

Query: 61  LLGNALDWDTFMDTMESLNFHEKKLIFFTVNENSNFSSSGGSHWSLLVYCRKRNLFMHYD 120
            +    +       +E L+   K+++F  +N+NSN  ++GG+HWSLLVY + +N F HYD
Sbjct: 61  FIKCTSNPAEIAMFLEPLDLPNKRVVFLAINDNSN-QAAGGTHWSLLVYLQDKNSFFHYD 120

Query: 121 SLHMMNNESAFAIYKFVNSYISY--DSVKFMECRAPRQRNGYDCGLYVMEIARVIYERYF 176
           S    N+  A  + + + +++    D + F+E +AP Q+N YDCG+YV+     + + +F
Sbjct: 121 SHSRSNSVHAKQVAEKLEAFLGRKGDKLAFVEEKAPAQQNSYDCGMYVICNTEALCQNFF 179

[Close]

BLAST of Carg11234-RA vs. Swiss-Prot
Match: sp|Q9D2Z4|SENP8_MOUSE (Sentrin-specific protease 8 OS=Mus musculus OX=10090 GN=Senp8 PE=1 SV=2)

HSP 1 Score: 99.4 bits (246), Expect = 5.9e-20
Identity = 59/169 (34.91%), Postives = 93/169 (55.03%), Query Frame = 0


Query: 1   MDLKFLIYNGVVLRRSDVRSLEDCNYLNDVIIDFYFAYLFESH---QSDDILLLPTSISF 60
           MD   L Y   +LR+SDV  L+  ++LND II F F Y   S     SD +  +   ++ 
Sbjct: 1   MDPVVLSYMDSLLRQSDVSLLDPPSWLNDHIIGFAFEYFANSQFHDCSDHVCFISPEVTQ 60

Query: 61  LLGNALDWDTFMDTMESLNFHEKKLIFFTVNENSNFSSSGGSHWSLLVYCRKRNLFMHYD 120
            +            +E L+   K+++F  +N+NSN  ++GG+HWSLLVY + +N F HYD
Sbjct: 61  FIKCTSSPAEIAMFLEPLDLPHKRVVFLAINDNSN-QAAGGTHWSLLVYLQDKNSFFHYD 120

Query: 121 SLHMMNNESAFAIYKFVNSYISY--DSVKFMECRAPRQRNGYDCGLYVM 165
           S    N+  A  + + + +++    D + F+E +AP Q N YDCG+YV+
Sbjct: 121 SHSRSNSIHAKQVAEKLKAFLGSKGDKLVFVEEKAPAQENSYDCGMYVI 168

[Close]

BLAST of Carg11234-RA vs. Swiss-Prot
Match: sp|Q54XR2|SENP8_DICDI (Probable sentrin-specific protease 8 OS=Dictyostelium discoideum OX=44689 GN=senp8 PE=3 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 1.9e-15
Identity = 57/174 (32.76%), Postives = 98/174 (56.32%), Query Frame = 0


Query: 8   YNGVVLRRSDVRSLEDCNYLNDVIIDFYFAYLFESHQS------DDILLLPTSISFLLGN 67
           YN   + +SD+  L+   +LND II FY  +L +  +       + +LLL  S+ F   +
Sbjct: 9   YNDSAIYQSDINILKSNQWLNDSIISFYLEWLKDGGEDNKNKIPNQVLLLSPSVVFCC-S 68

Query: 68  ALDWDTFMDTM--ESLNFHEKKLIFFTVNENSNFS-SSGGSHWSLLVYCRKRNLFMHYDS 127
            ++ +  +  M  + L+   K++IFF +  N + +   GG+HWSLL++ +  N F++YDS
Sbjct: 69  FVESEQEIQLMFEQPLSLKTKEVIFFPLTNNRDPNVIGGGTHWSLLIFIKSLNKFIYYDS 128

Query: 128 LHMMNNESA-FAIYKF---VNSYISYDSVK--FMECRAPRQRNGYDCGLYVMEI 167
           ++  N+  A F I KF   ++S     ++K   +  + P+Q+NGYDCGLYV+ I
Sbjct: 129 INSFNSSDAIFIISKFKFLLSSPPPKTNLKEFLINQKTPQQQNGYDCGLYVLSI 181

[Close]

BLAST of Carg11234-RA vs. TrEMBL
Match: tr|A0A2G5D630|A0A2G5D630_AQUCA (Uncharacterized protein OS=Aquilegia coerulea OX=218851 GN=AQUCO_02700117v1 PE=4 SV=1)

HSP 1 Score: 160.6 bits (405), Expect = 4.3e-36
Identity = 88/179 (49.16%), Postives = 117/179 (65.36%), Query Frame = 0


Query: 4   KFLIYNGVVLRRSDVRSLEDCNYLNDVIIDFYFAYLFESHQSDDILLLPTSISFLLGNAL 63
           K L YN VVLRRSD+  L   ++LND II+FYF+YL   + S+DILL+P SISF + N  
Sbjct: 9   KILSYNDVVLRRSDLDILSGPHFLNDRIIEFYFSYLASCYASEDILLVPPSISFWISNCP 68

Query: 64  DWDTFMDTMESLNFHEKKLIFFTVNENSNFS-SSGGSHWSLLVYCRKRNLFMHYDSLHMM 123
           D ++  D +E L F +KK++ F VN N + S + GGSHWSLL Y RK+N+F+H+DSL   
Sbjct: 69  DIESLKDFVEPLKFPDKKMVIFPVNNNDDVSMAEGGSHWSLLAYERKKNVFVHHDSLASS 128

Query: 124 NNESAFAIYKFVNSYISYDSVK----FMEC-RAPRQRNGYDCGLYVMEIARVIYERYFN 177
           N   A  +YK V  ++     K    F++C  +P+Q NGYDCGLYV  IA+VI E Y N
Sbjct: 129 NRCHAKRLYKGVVKFMGISDSKVDAPFIDCTTSPQQMNGYDCGLYVTAIAKVICEWYVN 187

[Close]

BLAST of Carg11234-RA vs. TrEMBL
Match: tr|A0A2G9G856|A0A2G9G856_9LAMI (Sentrin-specific cysteine protease (Ulp1 family) OS=Handroanthus impetiginosus OX=429701 GN=CDL12_26086 PE=4 SV=1)

HSP 1 Score: 156.4 bits (394), Expect = 8.2e-35
Identity = 84/175 (48.00%), Postives = 115/175 (65.71%), Query Frame = 0


Query: 2   DLKFLIYNGVVLRRSDVRSLEDCNYLNDVIIDFYFAYLFESHQSDDILLLPTSISFLLGN 61
           D K L YN VVLRRSD+  L    +LND II+FYF+YL   + S+DILL+P SI+F +  
Sbjct: 24  DEKILSYNDVVLRRSDLDILSGPYFLNDRIIEFYFSYLTSCYSSEDILLVPPSIAFWIKE 83

Query: 62  ALDWDTFMDTMESLNFHEKKLIFFTVNENSNFS-SSGGSHWSLLVYCRKRNLFMHYDSLH 121
             D  +  D +E L+  +KKLI F +N+N++ S + GGSHWSLL + R  N+F+H+DS  
Sbjct: 84  CPDVASLKDFVEPLHLSQKKLIIFPINDNADVSMAEGGSHWSLLAFERNANVFVHHDSSG 143

Query: 122 MMNNESAFAIYKFVNSYISYDSVKFMECRA-PRQRNGYDCGLYVMEIARVIYERY 175
            +N   A  +Y+ V SYI+    K++EC + P+Q NGYDCGLYV  IAR I + Y
Sbjct: 144 GINGAHAKRVYRTVVSYIASSDAKYVECSSTPKQVNGYDCGLYVTAIARAICDWY 198

[Close]

BLAST of Carg11234-RA vs. TrEMBL
Match: tr|A0A2K3PDX9|A0A2K3PDX9_TRIPR (NEDD8-specific protease 1-like protein OS=Trifolium pratense OX=57577 GN=L195_g010164 PE=4 SV=1)

HSP 1 Score: 154.1 bits (388), Expect = 4.1e-34
Identity = 85/181 (46.96%), Postives = 112/181 (61.88%), Query Frame = 0


Query: 2   DLKFLIYNGVVLRRSDVRSLEDCNYLNDVIIDFYFAYLFESHQSDDILLLPTSISFLLGN 61
           D K L YN VVLRRSD+  L    +LND II+FY +YL   H S DILL+P SI+F +  
Sbjct: 7   DEKILSYNDVVLRRSDLDILSGQYFLNDRIIEFYLSYLSSGHPSQDILLVPPSIAFWIIQ 66

Query: 62  ALDWDTFMDTMESLNFHEKKLIFFTVNENSNFS-SSGGSHWSLLVYCRKRNLFMHYDSLH 121
               +   D ++ L+  +K L+ F +N+N N S + GGSHWSLL Y R  NLF+H+DS  
Sbjct: 67  CPVAEAMRDFLDPLHLSDKALVLFPINDNDNVSMAEGGSHWSLLAYYRNANLFVHHDSCR 126

Query: 122 MMNNESAFAIYKFVNSYI----SYDSVKFMECR-APRQRNGYDCGLYVMEIARVIYERYF 177
            MN   A  +YK V  Y+    S    +++EC  +PRQ+NGYDCGLYV  IARVI + + 
Sbjct: 127 SMNASPAKKLYKAVVGYMGLSESGSKARYLECTDSPRQKNGYDCGLYVTAIARVICDWHV 186

[Close]

BLAST of Carg11234-RA vs. TrEMBL
Match: tr|A0A1S2YKG3|A0A1S2YKG3_CICAR (NEDD8-specific protease 1 OS=Cicer arietinum OX=3827 GN=LOC101498866 PE=4 SV=1)

HSP 1 Score: 154.1 bits (388), Expect = 4.1e-34
Identity = 88/181 (48.62%), Postives = 113/181 (62.43%), Query Frame = 0


Query: 2   DLKFLIYNGVVLRRSDVRSLEDCNYLNDVIIDFYFAYLFESHQSDDILLLPTSISFLLGN 61
           D K L YN VVLRRSD+  L    YLND II+FY +YL   H S DILL+P SI+F +  
Sbjct: 7   DEKILSYNDVVLRRSDLDILGGPYYLNDRIIEFYLSYLSSCHPSQDILLVPPSIAFWIIQ 66

Query: 62  ALDWDTFMDTMESLNFHEKKLIFFTVNENSNFSSS-GGSHWSLLVYCRKRNLFMHYDSLH 121
               ++F D +E L   +K LI F +N+N + +++ GGSHWSLL Y R  N F+H+DS  
Sbjct: 67  CPVAESFKDFLEPLQLSDKTLILFPINDNDDVNTAEGGSHWSLLAYYRNANQFVHHDSFI 126

Query: 122 MMNNESAFAIYKFVNSYI----SYDSVKFMEC-RAPRQRNGYDCGLYVMEIARVIYERYF 177
            MN E A  +YK V  Y+    S    +F+EC  +PRQ NGYDCGLYV  IARVI + + 
Sbjct: 127 NMNAEPAKKLYKAVLGYVGLSESGTEARFLECTNSPRQVNGYDCGLYVTAIARVICDWHV 186

[Close]

BLAST of Carg11234-RA vs. TrEMBL
Match: tr|A0A061GZJ1|A0A061GZJ1_THECC (Cysteine proteinases superfamily protein OS=Theobroma cacao OX=3641 GN=TCM_041110 PE=4 SV=1)

HSP 1 Score: 153.7 bits (387), Expect = 5.3e-34
Identity = 86/176 (48.86%), Postives = 107/176 (60.80%), Query Frame = 0


Query: 2   DLKFLIYNGVVLRRSDVRSLEDCNYLNDVIIDFYFAYLFESHQSDDILLLPTSISFLLGN 61
           D K L YN VVLR+SD+  L    YLND II+FYF+ L  SH S DILLL  SI+F + N
Sbjct: 7   DEKILSYNDVVLRQSDLEILSGPYYLNDRIIEFYFSLLSSSHPSQDILLLSPSIAFWIAN 66

Query: 62  ALDWDTFMDTMESLNFHEKKLIFFTVNENSNFS-SSGGSHWSLLVYCRKRNLFMHYDSLH 121
             D     D +E L   +KKL+ F VN N + S + GGSHWSLL YCR  N+F+H+DS  
Sbjct: 67  CPDVGGLKDFLEPLKLPDKKLVIFPVNNNDDVSVAEGGSHWSLLAYCRSANVFVHHDSNG 126

Query: 122 MMNNESAFAIYK----FVNSYISYDSVKFMEC-RAPRQRNGYDCGLYVMEIARVIY 172
            MN   A  ++K    FV    S  + K++EC   P+Q NGYDCGLYV   AR I+
Sbjct: 127 QMNKRHAMRLFKSVVGFVGDSSSACNAKYLECIGTPQQVNGYDCGLYVTVTARTIH 182

The following BLAST results are available for this feature:

BLAST of Carg11234-RA vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022988745.1	2.7e-106	90.48	NEDD8-specific protease 1-like [Cucurbita maxima]	[more]
XP_022942008.1	8.7e-105	89.57	NEDD8-specific protease 1-like [Cucurbita moschata]	[more]
XP_023526001.1	8.7e-81	93.13	NEDD8-specific protease 1-like [Cucurbita pepo subsp. pepo]	[more]
XP_022953908.1	2.4e-78	69.05	NEDD8-specific protease 1-like [Cucurbita moschata]	[more]
XP_023549404.1	3.8e-76	67.62	NEDD8-specific protease 1-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Carg11234-RA vs. TAIR10


Match Name	E-value	Identity	Description
AT5G60190.1	1.5e-34	41.71	Cysteine proteinases superfamily protein	[more]
AT1G09730.1	2.5e-05	22.73	Cysteine proteinases superfamily protein	[more]

BLAST of Carg11234-RA vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q9LSS7\|RUBP1_ARATH	2.7e-33	41.71	NEDD8-specific protease 1 OS=Arabidopsis thaliana OX=3702 GN=NEDP1 PE=2 SV=1	[more]
sp\|Q5FVJ8\|SENP8_RAT	6.9e-21	36.47	Sentrin-specific protease 8 OS=Rattus norvegicus OX=10116 GN=Senp8 PE=2 SV=1	[more]
sp\|Q96LD8\|SENP8_HUMAN	9.0e-21	33.33	Sentrin-specific protease 8 OS=Homo sapiens OX=9606 GN=SENP8 PE=1 SV=1	[more]
sp\|Q9D2Z4\|SENP8_MOUSE	5.9e-20	34.91	Sentrin-specific protease 8 OS=Mus musculus OX=10090 GN=Senp8 PE=1 SV=2	[more]
sp\|Q54XR2\|SENP8_DICDI	1.9e-15	32.76	Probable sentrin-specific protease 8 OS=Dictyostelium discoideum OX=44689 GN=sen...	[more]

BLAST of Carg11234-RA vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A2G5D630\|A0A2G5D630_AQUCA	4.3e-36	49.16	Uncharacterized protein OS=Aquilegia coerulea OX=218851 GN=AQUCO_02700117v1 PE=4...	[more]
tr\|A0A2G9G856\|A0A2G9G856_9LAMI	8.2e-35	48.00	Sentrin-specific cysteine protease (Ulp1 family) OS=Handroanthus impetiginosus O...	[more]
tr\|A0A2K3PDX9\|A0A2K3PDX9_TRIPR	4.1e-34	46.96	NEDD8-specific protease 1-like protein OS=Trifolium pratense OX=57577 GN=L195_g0...	[more]
tr\|A0A1S2YKG3\|A0A1S2YKG3_CICAR	4.1e-34	48.62	NEDD8-specific protease 1 OS=Cicer arietinum OX=3827 GN=LOC101498866 PE=4 SV=1	[more]
tr\|A0A061GZJ1\|A0A061GZJ1_THECC	5.3e-34	48.86	Cysteine proteinases superfamily protein OS=Theobroma cacao OX=3641 GN=TCM_04111...	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **Biological Process**
Term	Definition
GO:0006508	proteolysis

Vocabulary: **Molecular Function**
Term	Definition
GO:0008234	cysteine-type peptidase activity

Vocabulary: **INTERPRO**
Term	Definition
IPR038765	Papain_like_cys_pep_sf
IPR003653	Peptidase_C48_C

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0008234	cysteine-type peptidase activity

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Carg11234	Carg11234	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg11234-RA.exon.1	Carg11234-RA.exon.1	exon
Carg11234-RA.exon.2	Carg11234-RA.exon.2	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg11234-RA.CDS.1	Carg11234-RA.CDS.1	CDS
Carg11234-RA.CDS.2	Carg11234-RA.CDS.2	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Carg11234-RA	Carg11234-RA-protein	polypeptide

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003653	Ulp1 protease family, C-terminal catalytic domain	PFAM	PF02902	Peptidase_C48	coord: 27..173 e-value: 4.0E-12 score: 46.3
IPR003653	Ulp1 protease family, C-terminal catalytic domain	PROSITE	PS50600	ULP_PROTEASE	coord: 11..170 score: 16.999
None	No IPR available	GENE3D	G3DSA:3.40.395.10		coord: 1..211 e-value: 6.4E-53 score: 181.5
None	No IPR available	PANTHER	PTHR12606	SENTRIN/SUMO-SPECIFIC PROTEASE	coord: 8..177
IPR038765	Papain-like cysteine peptidase superfamily	SUPERFAMILY	SSF54001	Cysteine proteinases	coord: 5..180