Carg10495-RA (mRNA) Silver-seed gourd

NameCarg10495-RA
TypemRNA
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionCASP-like protein
LocationCucurbita_argyrosperma_scaffold_098 : 472646 .. 473781 (+)
Sequence length639
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGAACGCCGAATCCTCTGCCATCAATGTTGCCTCCGAGACCACCAACAGCGGCCCAATTGGGAAGAAAAAGAGCACGACCCCGTTGCTAGCGGCGGAGGTCCCAATCCGGCCAGACAGGGGAACCCACCGGATGAAACGTGGGCTTGCCATTTTCGACATCGTTCTGAGAATCGGCGCCCTTTCCTCGGCGCTGGCGGCGGCAGCCACCATGGGAACCAGTGGCCAAACGTTGCCGTTTTTCACTCAGTTCTTCCAGTTCGAGGCTAGCTACGACGATTTGCCCACTTTCCAGTTGGGTGGTTTTCATTCTTTTTTAAAAATACTCTTCTTATTGCGATTGAATTACTTAAACAAATAATTGAATTTTTTAATTGAAATTTGCAGGTTTTTTGTGATCGTAATGGGAATCGTTGCGGGATATGTGGTTCTATCACTTCCATTCTCAATTGTTTGCATTATTCGTCCCCATGCTGCTGGGCCTCGCCTTCTCCTCTTGATTTTGGACTCCGTAAGTATTCACTTAAAACTCTCGAAACTACGACTCTCCACGATAGTATGATATTGTCCACTTTGATGCTTTGAGCTCCCTCAAAAAACCTCATACGATCCTCAAAAATGACTGTTAGGAACCACGACTATCTACAATTGTATGATATTATCTATTTTGAGTATAAGCTCTCGTGATTTTGTTTCGAGCTTTCCCAATGTTAAGAACCATGACTCTCTACAATGATTTGATATTCTTAGGAACCACGACTCTTCGCAGTAGCATGATGTTGTTCACTTTGAGAATAAGCTCTCGTGACTACATTGGGCTTCTCCCAAAGACCTCATAAGACCTCGTAGCTTACTTATACACCCATGATCATTTTCTAGGACTCCATTAAAGAAAATATATGTCGTGGTTTGCAGGTGGCTTTGGCGTTGAGCACAGCGGCAGCAGGAGCGGCGGCCGCAGTGGTGGCGCTTGCACACAGTGGGAACTCAAGCACGAACTGGCTAGCAGTGTGCAACCAATTTGGGGACTTTTGCCAACAAGCGAGTGGGGCTGTGGTGGGCTCTTTTGCTGCTGTTTTGCTCTTCCTCCTACTCATCTTGTTCTCTGCCTTGTCCCTCAAGAGTGCCCATTGA

mRNA sequence

ATGAAGAACGCCGAATCCTCTGCCATCAATGTTGCCTCCGAGACCACCAACAGCGGCCCAATTGGGAAGAAAAAGAGCACGACCCCGTTGCTAGCGGCGGAGGTCCCAATCCGGCCAGACAGGGGAACCCACCGGATGAAACGTGGGCTTGCCATTTTCGACATCGTTCTGAGAATCGGCGCCCTTTCCTCGGCGCTGGCGGCGGCAGCCACCATGGGAACCAGTGGCCAAACGTTGCCGTTTTTCACTCAGTTCTTCCAGTTCGAGGCTAGCTACGACGATTTGCCCACTTTCCAGTTTTTTGTGATCGTAATGGGAATCGTTGCGGGATATGTGGTTCTATCACTTCCATTCTCAATTGTTTGCATTATTCGTCCCCATGCTGCTGGGCCTCGCCTTCTCCTCTTGATTTTGGACTCCGTGGCTTTGGCGTTGAGCACAGCGGCAGCAGGAGCGGCGGCCGCAGTGGTGGCGCTTGCACACAGTGGGAACTCAAGCACGAACTGGCTAGCAGTGTGCAACCAATTTGGGGACTTTTGCCAACAAGCGAGTGGGGCTGTGGTGGGCTCTTTTGCTGCTGTTTTGCTCTTCCTCCTACTCATCTTGTTCTCTGCCTTGTCCCTCAAGAGTGCCCATTGA

Coding sequence (CDS)

ATGAAGAACGCCGAATCCTCTGCCATCAATGTTGCCTCCGAGACCACCAACAGCGGCCCAATTGGGAAGAAAAAGAGCACGACCCCGTTGCTAGCGGCGGAGGTCCCAATCCGGCCAGACAGGGGAACCCACCGGATGAAACGTGGGCTTGCCATTTTCGACATCGTTCTGAGAATCGGCGCCCTTTCCTCGGCGCTGGCGGCGGCAGCCACCATGGGAACCAGTGGCCAAACGTTGCCGTTTTTCACTCAGTTCTTCCAGTTCGAGGCTAGCTACGACGATTTGCCCACTTTCCAGTTTTTTGTGATCGTAATGGGAATCGTTGCGGGATATGTGGTTCTATCACTTCCATTCTCAATTGTTTGCATTATTCGTCCCCATGCTGCTGGGCCTCGCCTTCTCCTCTTGATTTTGGACTCCGTGGCTTTGGCGTTGAGCACAGCGGCAGCAGGAGCGGCGGCCGCAGTGGTGGCGCTTGCACACAGTGGGAACTCAAGCACGAACTGGCTAGCAGTGTGCAACCAATTTGGGGACTTTTGCCAACAAGCGAGTGGGGCTGTGGTGGGCTCTTTTGCTGCTGTTTTGCTCTTCCTCCTACTCATCTTGTTCTCTGCCTTGTCCCTCAAGAGTGCCCATTGA

Protein sequence

MKNAESSAINVASETTNSGPIGKKKSTTPLLAAEVPIRPDRGTHRMKRGLAIFDIVLRIGALSSALAAAATMGTSGQTLPFFTQFFQFEASYDDLPTFQFFVIVMGIVAGYVVLSLPFSIVCIIRPHAAGPRLLLLILDSVALALSTAAAGAAAAVVALAHSGNSSTNWLAVCNQFGDFCQQASGAVVGSFAAVLLFLLLILFSALSLKSAH
BLAST of Carg10495-RA vs. NCBI nr
Match: XP_023544952.1 (casparian strip membrane protein 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 337.4 bits (864), Expect = 3.5e-89
Identity = 211/212 (99.53%), Postives = 212/212 (100.00%), Query Frame = 0

Query: 1   MKNAESSAINVASETTNSGPIGKKKSTTPLLAAEVPIRPDRGTHRMKRGLAIFDIVLRIG 60
           MKNAESSAINVASETTNSGPIGKKKSTTPLLAAEVPIRPDRGTHRMKRGLAIFDIVLRIG
Sbjct: 1   MKNAESSAINVASETTNSGPIGKKKSTTPLLAAEVPIRPDRGTHRMKRGLAIFDIVLRIG 60

Query: 61  ALSSALAAAATMGTSGQTLPFFTQFFQFEASYDDLPTFQFFVIVMGIVAGYVVLSLPFSI 120
           ALSSALAAAATMGTSGQTLPFFTQFFQFEASYDDLPTFQFFVIVMGIVAGYVVLSLPFSI
Sbjct: 61  ALSSALAAAATMGTSGQTLPFFTQFFQFEASYDDLPTFQFFVIVMGIVAGYVVLSLPFSI 120

Query: 121 VCIIRPHAAGPRLLLXXXXXXXXXXXXXXXXXXXXXXXXXXSGNSSTNWLAVCNQFGDFC 180
           VCIIRPHAAGPRLLLXXXXXXXXXXXXXXXXXXXXXXXXXXSGNSSTNWLAVCNQFGDFC
Sbjct: 121 VCIIRPHAAGPRLLLXXXXXXXXXXXXXXXXXXXXXXXXXXSGNSSTNWLAVCNQFGDFC 180

Query: 181 QQASGAVVGSFAAVLLFLLLILFSALSLKSAH 213
           QQASGAVVGSFAAVLLFLLLILFSALSLK+AH
Sbjct: 181 QQASGAVVGSFAAVLLFLLLILFSALSLKNAH 212

BLAST of Carg10495-RA vs. NCBI nr
Match: XP_022977901.1 (casparian strip membrane protein 1 [Cucurbita maxima])

HSP 1 Score: 336.7 bits (862), Expect = 5.9e-89
Identity = 210/212 (99.06%), Postives = 212/212 (100.00%), Query Frame = 0

Query: 1   MKNAESSAINVASETTNSGPIGKKKSTTPLLAAEVPIRPDRGTHRMKRGLAIFDIVLRIG 60
           MKNAESSAINVASETTNSGPIGKKKSTTPLLAAEVPIRPDRGTHRMKRGLAIFDIVLRIG
Sbjct: 1   MKNAESSAINVASETTNSGPIGKKKSTTPLLAAEVPIRPDRGTHRMKRGLAIFDIVLRIG 60

Query: 61  ALSSALAAAATMGTSGQTLPFFTQFFQFEASYDDLPTFQFFVIVMGIVAGYVVLSLPFSI 120
           ALSSALAAAATMGTSGQTLPFFTQFFQFEASYDDLPTFQFFVIVMGIVAGYVVLS+PFSI
Sbjct: 61  ALSSALAAAATMGTSGQTLPFFTQFFQFEASYDDLPTFQFFVIVMGIVAGYVVLSIPFSI 120

Query: 121 VCIIRPHAAGPRLLLXXXXXXXXXXXXXXXXXXXXXXXXXXSGNSSTNWLAVCNQFGDFC 180
           VCIIRPHAAGPRLLLXXXXXXXXXXXXXXXXXXXXXXXXXXSGNSSTNWLAVCNQFGDFC
Sbjct: 121 VCIIRPHAAGPRLLLXXXXXXXXXXXXXXXXXXXXXXXXXXSGNSSTNWLAVCNQFGDFC 180

Query: 181 QQASGAVVGSFAAVLLFLLLILFSALSLKSAH 213
           QQASGAVVGSFAAVLLFLLLILFSALSLK+AH
Sbjct: 181 QQASGAVVGSFAAVLLFLLLILFSALSLKNAH 212

BLAST of Carg10495-RA vs. NCBI nr
Match: XP_022926164.1 (casparian strip membrane protein 1-like [Cucurbita moschata])

HSP 1 Score: 334.3 bits (856), Expect = 2.9e-88
Identity = 210/212 (99.06%), Postives = 211/212 (99.53%), Query Frame = 0

Query: 1   MKNAESSAINVASETTNSGPIGKKKSTTPLLAAEVPIRPDRGTHRMKRGLAIFDIVLRIG 60
           MKNAESSAINVASETTNSG IGKKKSTTPLLAAEVPIRPDRGTHRMKRGLAIFDIVLRIG
Sbjct: 1   MKNAESSAINVASETTNSGSIGKKKSTTPLLAAEVPIRPDRGTHRMKRGLAIFDIVLRIG 60

Query: 61  ALSSALAAAATMGTSGQTLPFFTQFFQFEASYDDLPTFQFFVIVMGIVAGYVVLSLPFSI 120
           ALSSALAAAATMGTSGQTLPFFTQFFQFEASYDDLPTFQFFVIVMGIVAGYVVLSLPFSI
Sbjct: 61  ALSSALAAAATMGTSGQTLPFFTQFFQFEASYDDLPTFQFFVIVMGIVAGYVVLSLPFSI 120

Query: 121 VCIIRPHAAGPRLLLXXXXXXXXXXXXXXXXXXXXXXXXXXSGNSSTNWLAVCNQFGDFC 180
           VCIIRPHAAGPRLLLXXXXXXXXXXXXXXXXXXXXXXXXXXSGNSSTNWLAVCNQFGDFC
Sbjct: 121 VCIIRPHAAGPRLLLXXXXXXXXXXXXXXXXXXXXXXXXXXSGNSSTNWLAVCNQFGDFC 180

Query: 181 QQASGAVVGSFAAVLLFLLLILFSALSLKSAH 213
           QQASGAVVGSFAAVLLFLLLILFSALSLK+AH
Sbjct: 181 QQASGAVVGSFAAVLLFLLLILFSALSLKNAH 212

BLAST of Carg10495-RA vs. NCBI nr
Match: XP_022133169.1 (casparian strip membrane protein 1 [Momordica charantia])

HSP 1 Score: 299.3 bits (765), Expect = 1.0e-77
Identity = 172/208 (82.69%), Postives = 183/208 (87.98%), Query Frame = 0

Query: 2   KNAESSAINVASETTNSGPIGKKKSTTPLLAAEVPIRPDRGTHRMKRGLAIFDIVLRIGA 61
           KN+ES+AI+VA E  NSGPIGKKKSTTPLLAA VP+R DRGTHRMKRGLAIFD VLRIGA
Sbjct: 3   KNSESTAIDVAPENPNSGPIGKKKSTTPLLAAPVPVRQDRGTHRMKRGLAIFDFVLRIGA 62

Query: 62  LSSALAAAATMGTSGQTLPFFTQFFQFEASYDDLPTFQFFVIVMGIVAGYVVLSLPFSIV 121
           L++ALAAAA MGTS +TLPFFTQFFQFEASYDDLPTFQFFVI MGIVAGYVVLS+PFSIV
Sbjct: 63  LAAALAAAAAMGTSDETLPFFTQFFQFEASYDDLPTFQFFVIAMGIVAGYVVLSIPFSIV 122

Query: 122 CIIRPHAAGPRLLLXXXXXXXXXXXXXXXXXXXXXXXXXXSGNSSTNWLAVCNQFGDFCQ 181
           CI+RPHA GPRLLL          XXXXXXXXXXX     SGNSSTNWLA+CNQFGDFCQ
Sbjct: 123 CIVRPHAVGPRLLLLILDSVALTLXXXXXXXXXXXVSLAHSGNSSTNWLAICNQFGDFCQ 182

Query: 182 QASGAVVGSFAAVLLFLLLILFSALSLK 210
           QASGAV+GSFAAVLLFLLLILFSALSLK
Sbjct: 183 QASGAVIGSFAAVLLFLLLILFSALSLK 210

BLAST of Carg10495-RA vs. NCBI nr
Match: XP_004143331.1 (PREDICTED: casparian strip membrane protein 1 [Cucumis sativus] >KGN48281.1 hypothetical protein Csa_6G453800 [Cucumis sativus])

HSP 1 Score: 297.0 bits (759), Expect = 5.2e-77
Identity = 161/212 (75.94%), Postives = 174/212 (82.08%), Query Frame = 0

Query: 1   MKNAESSAINVASETTNSGPIGKKKSTTPLLAAEVPIRPDRGTHRMKRGLAIFDIVLRIG 60
           MK  ES++I++A ET NSGPIGKKKSTTPLLAA VP+  DRGTHRMKRGL+IFD VLRIG
Sbjct: 1   MKTGESTSIDIAPETNNSGPIGKKKSTTPLLAAPVPV-TDRGTHRMKRGLSIFDFVLRIG 60

Query: 61  ALSSALAAAATMGTSGQTLPFFTQFFQFEASYDDLPTFQFFVIVMGIVAGYVVLSLPFSI 120
            L+SALAAAATMGTS QTLPFFTQFFQFEASYDDLPTFQFFV+ M +VAGYVVLS+PFSI
Sbjct: 61  VLASALAAAATMGTSDQTLPFFTQFFQFEASYDDLPTFQFFVVAMAVVAGYVVLSIPFSI 120

Query: 121 VCIIRPHAAGPRLLLXXXXXXXXXXXXXXXXXXXXXXXXXXSGNSSTNWLAVCNQFGDFC 180
           VCIIRPHAAGPR+LL                          SGNSSTNWLAVCNQFGDFC
Sbjct: 121 VCIIRPHAAGPRVLLLILDSVALTLNTAAAGAAAAVVSLAHSGNSSTNWLAVCNQFGDFC 180

Query: 181 QQASGAVVGSFAAVLLFLLLILFSALSLKSAH 213
           QQASGAVVGSFAAVLLFLLLILFSALSLK++H
Sbjct: 181 QQASGAVVGSFAAVLLFLLLILFSALSLKNSH 211

BLAST of Carg10495-RA vs. TAIR10
Match: AT5G06200.1 (Uncharacterised protein family (UPF0497))

HSP 1 Score: 179.9 bits (455), Expect = 1.7e-45
Identity = 123/206 (59.71%), Postives = 154/206 (74.76%), Query Frame = 0

Query: 4   AESSAINVASETTNSGPIGKKKSTTPLLAAEVPIRPDRGTHRMKRGLAIFDIVLRIGALS 63
           ++S A++V +E+++       K   PLL      R   G+   KRGL+IFD +LR+ A+ 
Sbjct: 3   SDSIAVDVPAESSS-----VIKGKAPLLGL---ARDHTGSGGYKRGLSIFDFLLRLAAIV 62

Query: 64  SALAAAATMGTSGQTLPFFTQFFQFEASYDDLPTFQFFVIVMGIVAGYVVLSLPFSIVCI 123
           +ALAAAATMGTS +TLPFFTQF QFEASYDDLPTFQFFV+ + IVAGY+VLSLPFS+V I
Sbjct: 63  AALAAAATMGTSDETLPFFTQFLQFEASYDDLPTFQFFVVAIAIVAGYLVLSLPFSVVTI 122

Query: 124 IRPHAAGPRLLLXXXXXXXXXXXXXXXXXXXXXXXXXXSGNSSTNWLAVCNQFGDFCQQA 183
           +RP A  PRLLL    XXXXXXXXXXXXXXXXXXX   +GN++TNWL +C QFGDFCQ+ 
Sbjct: 123 VRPLAVAPRLLLLVLDXXXXXXXXXXXXXXXXXXXLAHNGNTNTNWLPICQQFGDFCQKT 182

Query: 184 SGAVVGSFAAVLLFLLLILFSALSLK 210
           SGAVV +FA+V    +L++ S +SLK
Sbjct: 183 SGAVVSAFASVTFLAILVVISGVSLK 200

BLAST of Carg10495-RA vs. TAIR10
Match: AT3G11550.1 (Uncharacterised protein family (UPF0497))

HSP 1 Score: 177.9 bits (450), Expect = 6.4e-45
Identity = 105/209 (50.24%), Postives = 135/209 (64.59%), Query Frame = 0

Query: 1   MKNAESSAINVASETTNSGPIGKKKSTTPLLAAEVPIRPDRGTHRMKRGLAIFDIVLRIG 60
           MKN ES+ I+V +E++++      K   PL+          G+    RGLAIFD +LR+ 
Sbjct: 1   MKN-ESTFIDVPAESSSA-----MKGKAPLIGV-ARDHTTSGSGGYNRGLAIFDFLLRLA 60

Query: 61  ALSSALAAAATMGTSGQTLPFFTQFFQFEASYDDLPTFQFFVIVMGIVAGYVVLSLPFSI 120
           A+ +ALAAAATMGTS +TLPFFTQF QFEASYDDLPTFQFFVI M +V GY+VLSLP S+
Sbjct: 61  AIVAALAAAATMGTSDETLPFFTQFLQFEASYDDLPTFQFFVIAMALVGGYLVLSLPISV 120

Query: 121 VCIIRPHAAGPRLLLXXXXXXXXXXXXXXXXXXXXXXXXXXSGNSSTNWLAVCNQFGDFC 180
           V I+RP A  PRLLL                          SGN +TNWL +C QFGDFC
Sbjct: 121 VTILRPLATAPRLLLLVLDTGVLALNTAAASSAAAISYLAHSGNQNTNWLPICQQFGDFC 180

Query: 181 QQASGAVVGSFAAVLLFLLLILFSALSLK 210
           Q++SGAVV +F +V+ F +L++ S ++LK
Sbjct: 181 QKSSGAVVSAFVSVVFFTILVVISGVALK 202

BLAST of Carg10495-RA vs. TAIR10
Match: AT2G36100.1 (Uncharacterised protein family (UPF0497))

HSP 1 Score: 162.2 bits (409), Expect = 3.6e-40
Identity = 85/163 (52.15%), Postives = 108/163 (66.26%), Query Frame = 0

Query: 47  KRGLAIFDIVLRIGALSSALAAAATMGTSGQTLPFFTQFFQFEASYDDLPTFQFFVIVMG 106
           KRGLAIFD +LR+ A++  + AA+ M T+ +TLPFFTQF QF+A YDDLP FQ+FVI + 
Sbjct: 42  KRGLAIFDFLLRLAAIAVTIGAASVMYTAEETLPFFTQFLQFQAGYDDLPAFQYFVIAVA 101

Query: 107 IVAGYVVLSLPFSIVCIIRPHAAGPRLLLXXXXXXXXXXXXXXXXXXXXXXXXXXSGNSS 166
           +VA Y+VLSLPFSIV I+RPHA  PRL+L                          +GN S
Sbjct: 102 VVASYLVLSLPFSIVSIVRPHAVAPRLILLICDTLVVTLNTSAAAAAASITYLAHNGNQS 161

Query: 167 TNWLAVCNQFGDFCQQASGAVVGSFAAVLLFLLLILFSALSLK 210
           TNWL +C QFGDFCQ  S AVV    A+L F++LI+ SA++LK
Sbjct: 162 TNWLPICQQFGDFCQNVSTAVVADSIAILFFIVLIIISAIALK 204

BLAST of Carg10495-RA vs. TAIR10
Match: AT2G27370.1 (Uncharacterised protein family (UPF0497))

HSP 1 Score: 157.1 bits (396), Expect = 1.2e-38
Identity = 89/163 (54.60%), Postives = 104/163 (63.80%), Query Frame = 0

Query: 47  KRGLAIFDIVLRIGALSSALAAAATMGTSGQTLPFFTQFFQFEASYDDLPTFQFFVIVMG 106
           KRG+AIFD VLR+ A  +A+AAAA M T+ +TLPFFTQF QF+A Y DLPT   FVIV  
Sbjct: 56  KRGVAIFDFVLRLIAAITAMAAAAKMATTEETLPFFTQFLQFQADYTDLPTMSSFVIVNS 115

Query: 107 IVAGYVVLSLPFSIVCIIRPHAAGPRLLLXXXXXXXXXXXXXXXXXXXXXXXXXXSGNSS 166
           IV GY+ LSLPFSIVCI+RP A  PRL L                          +GNSS
Sbjct: 116 IVGGYLTLSLPFSIVCILRPLAVPPRLFLILCDTVMMGLTLMAASASAAIVYLAHNGNSS 175

Query: 167 TNWLAVCNQFGDFCQQASGAVVGSFAAVLLFLLLILFSALSLK 210
           +NWL VC QFGDFCQ  SGAVV SF A  L + L++ SA +LK
Sbjct: 176 SNWLPVCQQFGDFCQGTSGAVVASFIAATLLMFLVILSAFALK 218

BLAST of Carg10495-RA vs. TAIR10
Match: AT5G15290.1 (Uncharacterised protein family (UPF0497))

HSP 1 Score: 114.8 bits (286), Expect = 6.6e-26
Identity = 61/167 (36.53%), Postives = 92/167 (55.09%), Query Frame = 0

Query: 46  MKRGLAIFDIVLRIGALSSALAAAATMGTSGQTLPFFTQFFQFEASYDDLPTFQFFVIVM 105
           M R +AI + +LRI A  + + +A  MGT+ +TLPFFTQF +F+A Y+DLP   FFV+  
Sbjct: 21  MSRRIAILEFILRIVAFFNTIGSAILMGTTHETLPFFTQFIRFQAEYNDLPALTFFVVAN 80

Query: 106 GIVAGYVVLSLPFSIVCIIRPHAAGPRLLLXXXXXXXXXXXXXXXXXXXXXXXXXXSGNS 165
            +V+GY++LSL  + V I++      R+LL                          +GN+
Sbjct: 81  AVVSGYLILSLTLAFVHIVKRKTQNTRILLIILDVAMLGLLTSGASSAAAIVYLAHNGNN 140

Query: 166 STNWLAVCNQFGDFCQQASGAVVGSFAAVLLFLLLILFSALSLKSAH 213
            TNW A+C QF  FC++ SG+++GSF A+         SA++L   H
Sbjct: 141 KTNWFAICQQFNSFCERISGSLIGSFIAIXXXXXXXXXSAIALSRRH 187

BLAST of Carg10495-RA vs. Swiss-Prot
Match: sp|P0DI61|CASP1_CUCME (Casparian strip membrane protein 1 OS=Cucumis melo OX=3656 PE=2 SV=1)

HSP 1 Score: 295.0 bits (754), Expect = 6.4e-79
Identity = 161/212 (75.94%), Postives = 172/212 (81.13%), Query Frame = 0

Query: 1   MKNAESSAINVASETTNSGPIGKKKSTTPLLAAEVPIRPDRGTHRMKRGLAIFDIVLRIG 60
           MK  ES+AI++A ET NSGPIGKKKSTTPLLAA V   PDRGTHRMKRGLAIFD VLRIG
Sbjct: 1   MKTGESTAIDIAPETNNSGPIGKKKSTTPLLAAPV---PDRGTHRMKRGLAIFDFVLRIG 60

Query: 61  ALSSALAAAATMGTSGQTLPFFTQFFQFEASYDDLPTFQFFVIVMGIVAGYVVLSLPFSI 120
            L+SALAAAA MGTS QTLPFFTQFFQFEASYDDLPTFQFFV+ M +VAGYVVLS+PFSI
Sbjct: 61  VLASALAAAAAMGTSEQTLPFFTQFFQFEASYDDLPTFQFFVVAMAVVAGYVVLSIPFSI 120

Query: 121 VCIIRPHAAGPRLLLXXXXXXXXXXXXXXXXXXXXXXXXXXSGNSSTNWLAVCNQFGDFC 180
           VCIIRPHAAGPR+LL                          SGNSSTNWLA+CNQFGDFC
Sbjct: 121 VCIIRPHAAGPRVLLLILDSVALTLNTAAAGAAAAVVSLAHSGNSSTNWLAICNQFGDFC 180

Query: 181 QQASGAVVGSFAAVLLFLLLILFSALSLKSAH 213
           QQASGAVVGSFAAVLLFLLLILFSALSLK++H
Sbjct: 181 QQASGAVVGSFAAVLLFLLLILFSALSLKNSH 209

BLAST of Carg10495-RA vs. Swiss-Prot
Match: sp|B9IIR5|CASP2_POPTR (Casparian strip membrane protein 2 OS=Populus trichocarpa OX=3694 GN=POPTRDRAFT_576556 PE=2 SV=1)

HSP 1 Score: 199.5 bits (506), Expect = 3.7e-50
Identity = 110/185 (59.46%), Postives = 135/185 (72.97%), Query Frame = 0

Query: 25  KSTTPLLAAEVPIRPDRGTHRMKRGLAIFDIVLRIGALSSALAAAATMGTSGQTLPFFTQ 84
           K T PL+AA  P++   G    K+G+AIFD +LR+ A+++ALAAAA+MGTS +TLPFFTQ
Sbjct: 18  KGTAPLIAA--PMKEKGG---YKKGIAIFDFILRLAAIATALAAAASMGTSDETLPFFTQ 77

Query: 85  FFQFEASYDDLPTFQFFVIVMGIVAGYVVLSLPFSIVCIIRPHAAGPRLLLXXXXXXXXX 144
           FFQF+ASYDDLPTFQFFVI M IVAGY+VLSLPFSIV I+RPHAAGPRLLL         
Sbjct: 78  FFQFQASYDDLPTFQFFVIAMAIVAGYLVLSLPFSIVAIVRPHAAGPRLLLIILDTVALT 137

Query: 145 XXXXXXXXXXXXXXXXXSGNSSTNWLAVCNQFGDFCQQASGAVVGSFAAVLLFLLLILFS 204
                   X        +GNSSTNWLA+C QFGDFCQ+ SGAVV SF  V++F+ L++ S
Sbjct: 138 LNTAAGAAXAAIVYLAHNGNSSTNWLAICQQFGDFCQKNSGAVVASFITVVIFVFLLVLS 197

Query: 205 ALSLK 210
           A +L+
Sbjct: 198 AFALR 197

BLAST of Carg10495-RA vs. Swiss-Prot
Match: sp|B9IIR4|CASP3_POPTR (Casparian strip membrane protein 3 OS=Populus trichocarpa OX=3694 GN=POPTRDRAFT_907513 PE=3 SV=1)

HSP 1 Score: 197.6 bits (501), Expect = 1.4e-49
Identity = 119/204 (58.33%), Postives = 146/204 (71.57%), Query Frame = 0

Query: 6   SSAINVASETTNSGPIGKKKSTTPLLAAEVPIRPDRGTHRMKRGLAIFDIVLRIGALSSA 65
           S+ +++ ++T+ +      K T PL+AA   ++        K+GLAIFD+VLR+GA+ +A
Sbjct: 2   SARVDIPADTSAAA-----KGTAPLIAASTHVKGG-----YKKGLAIFDLVLRLGAVVTA 61

Query: 66  LAAAATMGTSGQTLPFFTQFFQFEASYDDLPTFQFFVIVMGIVAGYVVLSLPFSIVCIIR 125
           LAAAATMGT+ QTLPFFTQFFQF+ASYDDLPTFQFFVI M IV+GY+VLSLPFSIV IIR
Sbjct: 62  LAAAATMGTTDQTLPFFTQFFQFQASYDDLPTFQFFVIAMAIVSGYLVLSLPFSIVAIIR 121

Query: 126 PHAAGPRLLLXXXXXXXXXXXXXXXXXXXXXXXXXXSGNSSTNWLAVCNQFGDFCQQASG 185
           PHA GPRLLL             XXXXXX       +GNSS NWL +C QFGDFCQ+ASG
Sbjct: 122 PHATGPRLLLIILDTVALTLNTAXXXXXXAIVDLAQNGNSSANWLGICQQFGDFCQKASG 181

Query: 186 AVVGSFAAVLLFLLLILFSALSLK 210
           AVV SF A  + L LI+ SAL+L+
Sbjct: 182 AVVASFIAAGVLLFLIVISALALR 195

BLAST of Carg10495-RA vs. Swiss-Prot
Match: sp|B9HBX2|CASP1_POPTR (Casparian strip membrane protein 1 OS=Populus trichocarpa OX=3694 GN=POPTRDRAFT_763057 PE=2 SV=1)

HSP 1 Score: 190.7 bits (483), Expect = 1.7e-47
Identity = 114/206 (55.34%), Postives = 148/206 (71.84%), Query Frame = 0

Query: 4   AESSAINVASETTNSGPIGKKKSTTPLLAAEVPIRPDRGTHRMKRGLAIFDIVLRIGALS 63
           +ES+AI++   ++ +      K   PL+A     R ++G +R  +G+AIFD +LR+ A++
Sbjct: 3   SESAAIDIPESSSVA------KGKAPLIAVS---RNEKGGYR--KGIAIFDFILRLAAIA 62

Query: 64  SALAAAATMGTSGQTLPFFTQFFQFEASYDDLPTFQFFVIVMGIVAGYVVLSLPFSIVCI 123
           +ALAAAA MGTS +TLPFFTQFFQF+ASYDDLPTFQFFVI + IV GY+VLSLPFSIV I
Sbjct: 63  TALAAAAAMGTSDETLPFFTQFFQFQASYDDLPTFQFFVIAIAIVGGYLVLSLPFSIVAI 122

Query: 124 IRPHAAGPRLLLXXXXXXXXXXXXXXXXXXXXXXXXXXSGNSSTNWLAVCNQFGDFCQQA 183
           +RPHA GPRLLL              XXXXXXX     +GNS+TNWLA+C Q+GDFCQ+ 
Sbjct: 123 VRPHAVGPRLLLIILDAVALTLNTAAXXXXXXXVYLAHNGNSNTNWLAICQQYGDFCQKV 182

Query: 184 SGAVVGSFAAVLLFLLLILFSALSLK 210
           SGAVV SF  V++F+ LI+ SA +L+
Sbjct: 183 SGAVVASFITVVIFVFLIVLSAFALR 197

BLAST of Carg10495-RA vs. Swiss-Prot
Match: sp|B9SX12|CASP1_RICCO (Casparian strip membrane protein 1 OS=Ricinus communis OX=3988 GN=RCOM_1259250 PE=2 SV=1)

HSP 1 Score: 189.1 bits (479), Expect = 5.0e-47
Identity = 131/204 (64.22%), Postives = 157/204 (76.96%), Query Frame = 0

Query: 6   SSAINVASETTNSGPIGKKKSTTPLLAAEVPIRPDRGTHRMKRGLAIFDIVLRIGALSSA 65
           S+ I + +E   S  + K K+  PL+ A       +G +  K+G+AIFD +LR+GA+ SA
Sbjct: 2   STTIEIPAE---SSAVAKGKA--PLIGASSSSYEKKGGY--KKGIAIFDFILRLGAVISA 61

Query: 66  LAAAATMGTSGQTLPFFTQFFQFEASYDDLPTFQFFVIVMGIVAGYVVLSLPFSIVCIIR 125
           L+AAATMGTS +TLPFFTQFFQFEA YDD PTFQFFVI MG V GY+VLSLPFS+V IIR
Sbjct: 62  LSAAATMGTSDETLPFFTQFFQFEAGYDDFPTFQFFVIAMGFVGGYLVLSLPFSVVAIIR 121

Query: 126 PHAAGPRLLLXXXXXXXXXXXXXXXXXXXXXXXXXXSGNSSTNWLAVCNQFGDFCQQASG 185
           PHA G RLL XXXXXXXXXXXXXXXXXXXXXX    +GN S NWLAVC QFGDFCQ+ SG
Sbjct: 122 PHAVGIRLLXXXXXXXXXXXXXXXXXXXXXXXYLAHNGNQSANWLAVCQQFGDFCQKVSG 181

Query: 186 AVVGSFAAVLLFLLLILFSALSLK 210
            VV SF +VL+FLLL++ SA++L+
Sbjct: 182 GVVASFVSVLVFLLLVVMSAVALR 198

BLAST of Carg10495-RA vs. TrEMBL
Match: tr|A0A0A0KKJ8|A0A0A0KKJ8_CUCSA (CASP-like protein OS=Cucumis sativus OX=3659 GN=Csa_6G453800 PE=3 SV=1)

HSP 1 Score: 297.0 bits (759), Expect = 3.4e-77
Identity = 161/212 (75.94%), Postives = 174/212 (82.08%), Query Frame = 0

Query: 1   MKNAESSAINVASETTNSGPIGKKKSTTPLLAAEVPIRPDRGTHRMKRGLAIFDIVLRIG 60
           MK  ES++I++A ET NSGPIGKKKSTTPLLAA VP+  DRGTHRMKRGL+IFD VLRIG
Sbjct: 1   MKTGESTSIDIAPETNNSGPIGKKKSTTPLLAAPVPV-TDRGTHRMKRGLSIFDFVLRIG 60

Query: 61  ALSSALAAAATMGTSGQTLPFFTQFFQFEASYDDLPTFQFFVIVMGIVAGYVVLSLPFSI 120
            L+SALAAAATMGTS QTLPFFTQFFQFEASYDDLPTFQFFV+ M +VAGYVVLS+PFSI
Sbjct: 61  VLASALAAAATMGTSDQTLPFFTQFFQFEASYDDLPTFQFFVVAMAVVAGYVVLSIPFSI 120

Query: 121 VCIIRPHAAGPRLLLXXXXXXXXXXXXXXXXXXXXXXXXXXSGNSSTNWLAVCNQFGDFC 180
           VCIIRPHAAGPR+LL                          SGNSSTNWLAVCNQFGDFC
Sbjct: 121 VCIIRPHAAGPRVLLLILDSVALTLNTAAAGAAAAVVSLAHSGNSSTNWLAVCNQFGDFC 180

Query: 181 QQASGAVVGSFAAVLLFLLLILFSALSLKSAH 213
           QQASGAVVGSFAAVLLFLLLILFSALSLK++H
Sbjct: 181 QQASGAVVGSFAAVLLFLLLILFSALSLKNSH 211

BLAST of Carg10495-RA vs. TrEMBL
Match: tr|A0A1S3CHB9|A0A1S3CHB9_CUCME (CASP-like protein OS=Cucumis melo OX=3656 GN=LOC103500916 PE=3 SV=1)

HSP 1 Score: 295.0 bits (754), Expect = 1.3e-76
Identity = 161/212 (75.94%), Postives = 172/212 (81.13%), Query Frame = 0

Query: 1   MKNAESSAINVASETTNSGPIGKKKSTTPLLAAEVPIRPDRGTHRMKRGLAIFDIVLRIG 60
           MK  ES+AI++A ET NSGPIGKKKSTTPLLAA V   PDRGTHRMKRGLAIFD VLRIG
Sbjct: 1   MKTGESTAIDIAPETNNSGPIGKKKSTTPLLAAPV---PDRGTHRMKRGLAIFDFVLRIG 60

Query: 61  ALSSALAAAATMGTSGQTLPFFTQFFQFEASYDDLPTFQFFVIVMGIVAGYVVLSLPFSI 120
            L+SALAAAA MGTS QTLPFFTQFFQFEASYDDLPTFQFFV+ M +VAGYVVLS+PFSI
Sbjct: 61  VLASALAAAAAMGTSEQTLPFFTQFFQFEASYDDLPTFQFFVVAMAVVAGYVVLSIPFSI 120

Query: 121 VCIIRPHAAGPRLLLXXXXXXXXXXXXXXXXXXXXXXXXXXSGNSSTNWLAVCNQFGDFC 180
           VCIIRPHAAGPR+LL                          SGNSSTNWLA+CNQFGDFC
Sbjct: 121 VCIIRPHAAGPRVLLLILDSVALTLNTAAAGAAAAVVSLAHSGNSSTNWLAICNQFGDFC 180

Query: 181 QQASGAVVGSFAAVLLFLLLILFSALSLKSAH 213
           QQASGAVVGSFAAVLLFLLLILFSALSLK++H
Sbjct: 181 QQASGAVVGSFAAVLLFLLLILFSALSLKNSH 209

BLAST of Carg10495-RA vs. TrEMBL
Match: tr|A0A2K1XC97|A0A2K1XC97_POPTR (CASP-like protein OS=Populus trichocarpa OX=3694 GN=POPTR_016G075400v3 PE=3 SV=1)

HSP 1 Score: 199.5 bits (506), Expect = 7.5e-48
Identity = 110/185 (59.46%), Postives = 135/185 (72.97%), Query Frame = 0

Query: 25  KSTTPLLAAEVPIRPDRGTHRMKRGLAIFDIVLRIGALSSALAAAATMGTSGQTLPFFTQ 84
           K T PL+AA  P++   G    K+G+AIFD +LR+ A+++ALAAAA+MGTS +TLPFFTQ
Sbjct: 18  KGTAPLIAA--PMKEKGG---YKKGIAIFDFILRLAAIATALAAAASMGTSDETLPFFTQ 77

Query: 85  FFQFEASYDDLPTFQFFVIVMGIVAGYVVLSLPFSIVCIIRPHAAGPRLLLXXXXXXXXX 144
           FFQF+ASYDDLPTFQFFVI M IVAGY+VLSLPFSIV I+RPHAAGPRLLL         
Sbjct: 78  FFQFQASYDDLPTFQFFVIAMAIVAGYLVLSLPFSIVAIVRPHAAGPRLLLIILDTVALT 137

Query: 145 XXXXXXXXXXXXXXXXXSGNSSTNWLAVCNQFGDFCQQASGAVVGSFAAVLLFLLLILFS 204
                   X        +GNSSTNWLA+C QFGDFCQ+ SGAVV SF  V++F+ L++ S
Sbjct: 138 LNTAAGAAXAAIVYLAHNGNSSTNWLAICQQFGDFCQKNSGAVVASFITVVIFVFLLVLS 197

Query: 205 ALSLK 210
           A +L+
Sbjct: 198 AFALR 197

BLAST of Carg10495-RA vs. TrEMBL
Match: tr|M5VSP6|M5VSP6_PRUPE (CASP-like protein OS=Prunus persica OX=3760 GN=PRUPE_7G101300 PE=3 SV=1)

HSP 1 Score: 198.4 bits (503), Expect = 1.7e-47
Identity = 113/209 (54.07%), Postives = 141/209 (67.46%), Query Frame = 0

Query: 1   MKNAESSAINVASETTNSGPIGKKKSTTPLLAAEVPIRPDRGTHRMKRGLAIFDIVLRIG 60
           MK+ +S+ I+V     + G   K K         VP R ++G   M+RG+AI D+VLRIG
Sbjct: 1   MKSGDSTTIDVPHHEASGGAASKGKGA----ILTVPARQEKG--GMRRGMAIIDLVLRIG 60

Query: 61  ALSSALAAAATMGTSGQTLPFFTQFFQFEASYDDLPTFQFFVIVMGIVAGYVVLSLPFSI 120
           A+ +ALAAAATMGTS Q LPFFTQFFQFEASYDD+P+FQFF+I M +VAGY+VLS+PFSI
Sbjct: 61  AIVAALAAAATMGTSDQNLPFFTQFFQFEASYDDMPSFQFFLIAMSLVAGYLVLSVPFSI 120

Query: 121 VCIIRPHAAGPRLLLXXXXXXXXXXXXXXXXXXXXXXXXXXSGNSSTNWLAVCNQFGDFC 180
           V I+RPHA+G RLLL                          +GNSS+NWLA+CNQFGDFC
Sbjct: 121 VSIVRPHASGIRLLLLILDVVALTLATSAAGAATAIVYLAHNGNSSSNWLAICNQFGDFC 180

Query: 181 QQASGAVVGSFAAVLLFLLLILFSALSLK 210
           Q  SGAVV SF  V+ F+ LIL SA++L+
Sbjct: 181 QTVSGAVVASFVTVVTFMFLILLSAVALR 203

BLAST of Carg10495-RA vs. TrEMBL
Match: tr|A0A2K1XCA2|A0A2K1XCA2_POPTR (CASP-like protein OS=Populus trichocarpa OX=3694 GN=POPTR_016G075300v3 PE=3 SV=1)

HSP 1 Score: 197.2 bits (500), Expect = 3.7e-47
Identity = 118/204 (57.84%), Postives = 146/204 (71.57%), Query Frame = 0

Query: 6   SSAINVASETTNSGPIGKKKSTTPLLAAEVPIRPDRGTHRMKRGLAIFDIVLRIGALSSA 65
           S+ +++ ++T+ +      K T PL+AA   ++        K+GLAIFD+VLR+GA+ +A
Sbjct: 2   SARVDIPADTSAAA-----KGTAPLIAASTHVKGG-----YKKGLAIFDLVLRLGAVVTA 61

Query: 66  LAAAATMGTSGQTLPFFTQFFQFEASYDDLPTFQFFVIVMGIVAGYVVLSLPFSIVCIIR 125
           LAAAATMGT+ QTLPFFTQFFQF+ASYDDLPTFQFFVI M IV+GY++LSLPFSIV IIR
Sbjct: 62  LAAAATMGTTDQTLPFFTQFFQFQASYDDLPTFQFFVIAMAIVSGYLILSLPFSIVAIIR 121

Query: 126 PHAAGPRLLLXXXXXXXXXXXXXXXXXXXXXXXXXXSGNSSTNWLAVCNQFGDFCQQASG 185
           PHA GPRLLL             XXXXXX       +GNSS NWL +C QFGDFCQ+ASG
Sbjct: 122 PHATGPRLLLIILDTVALTLNTAXXXXXXAIVDLAQNGNSSANWLGICQQFGDFCQKASG 181

Query: 186 AVVGSFAAVLLFLLLILFSALSLK 210
           AVV SF A  + L LI+ SAL+L+
Sbjct: 182 AVVASFIAAGVLLFLIVISALALR 195

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023544952.13.5e-8999.53casparian strip membrane protein 1 [Cucurbita pepo subsp. pepo][more]
XP_022977901.15.9e-8999.06casparian strip membrane protein 1 [Cucurbita maxima][more]
XP_022926164.12.9e-8899.06casparian strip membrane protein 1-like [Cucurbita moschata][more]
XP_022133169.11.0e-7782.69casparian strip membrane protein 1 [Momordica charantia][more]
XP_004143331.15.2e-7775.94PREDICTED: casparian strip membrane protein 1 [Cucumis sativus] >KGN48281.1 hypo... [more]
Match NameE-valueIdentityDescription
AT5G06200.11.7e-4559.71Uncharacterised protein family (UPF0497)[more]
AT3G11550.16.4e-4550.24Uncharacterised protein family (UPF0497)[more]
AT2G36100.13.6e-4052.15Uncharacterised protein family (UPF0497)[more]
AT2G27370.11.2e-3854.60Uncharacterised protein family (UPF0497)[more]
AT5G15290.16.6e-2636.53Uncharacterised protein family (UPF0497)[more]
Match NameE-valueIdentityDescription
sp|P0DI61|CASP1_CUCME6.4e-7975.94Casparian strip membrane protein 1 OS=Cucumis melo OX=3656 PE=2 SV=1[more]
sp|B9IIR5|CASP2_POPTR3.7e-5059.46Casparian strip membrane protein 2 OS=Populus trichocarpa OX=3694 GN=POPTRDRAFT_... [more]
sp|B9IIR4|CASP3_POPTR1.4e-4958.33Casparian strip membrane protein 3 OS=Populus trichocarpa OX=3694 GN=POPTRDRAFT_... [more]
sp|B9HBX2|CASP1_POPTR1.7e-4755.34Casparian strip membrane protein 1 OS=Populus trichocarpa OX=3694 GN=POPTRDRAFT_... [more]
sp|B9SX12|CASP1_RICCO5.0e-4764.22Casparian strip membrane protein 1 OS=Ricinus communis OX=3988 GN=RCOM_1259250 P... [more]
Match NameE-valueIdentityDescription
tr|A0A0A0KKJ8|A0A0A0KKJ8_CUCSA3.4e-7775.94CASP-like protein OS=Cucumis sativus OX=3659 GN=Csa_6G453800 PE=3 SV=1[more]
tr|A0A1S3CHB9|A0A1S3CHB9_CUCME1.3e-7675.94CASP-like protein OS=Cucumis melo OX=3656 GN=LOC103500916 PE=3 SV=1[more]
tr|A0A2K1XC97|A0A2K1XC97_POPTR7.5e-4859.46CASP-like protein OS=Populus trichocarpa OX=3694 GN=POPTR_016G075400v3 PE=3 SV=1[more]
tr|M5VSP6|M5VSP6_PRUPE1.7e-4754.07CASP-like protein OS=Prunus persica OX=3760 GN=PRUPE_7G101300 PE=3 SV=1[more]
tr|A0A2K1XCA2|A0A2K1XCA2_POPTR3.7e-4757.84CASP-like protein OS=Populus trichocarpa OX=3694 GN=POPTR_016G075300v3 PE=3 SV=1[more]
The following terms have been associated with this mRNA:
Vocabulary: INTERPRO
TermDefinition
IPR006702CASP_dom
IPR006459CASP/CASPL
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0003674 molecular_function

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Carg10495Carg10495gene


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Carg10495-RACarg10495-RA-proteinpolypeptide


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg10495-RA.exon.1Carg10495-RA.exon.1exon
Carg10495-RA.exon.2Carg10495-RA.exon.2exon
Carg10495-RA.exon.3Carg10495-RA.exon.3exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg10495-RA.CDS.1Carg10495-RA.CDS.1CDS
Carg10495-RA.CDS.2Carg10495-RA.CDS.2CDS
Carg10495-RA.CDS.3Carg10495-RA.CDS.3CDS


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006459Casparian strip membrane proteinTIGRFAMTIGR01569TIGR01569coord: 55..207
e-value: 1.9E-40
score: 136.2
IPR006702Casparian strip membrane protein domainPFAMPF04535DUF588coord: 48..196
e-value: 1.2E-44
score: 151.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..26
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..24
NoneNo IPR availablePANTHERPTHR11615NITRATE, FROMATE, IRON DEHYDROGENASEcoord: 5..209
NoneNo IPR availablePANTHERPTHR11615:SF116CASPARIAN STRIP MEMBRANE PROTEIN 1-RELATEDcoord: 5..209