Carg10052-RA (mRNA) Silver-seed gourd

NameCarg10052-RA
TypemRNA
OrganismCucurbita argyrosperma (Silver-seed gourd)
Description3'-phosphoadenosine 5'-phosphosulfate synthase 2
LocationCucurbita_argyrosperma_scaffold_039 : 106098 .. 109398 (+)
Sequence length1866
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AGAAAAAAAAGTACCTTTTAGTTTTGGCGCATAATAAACCGAATTGATCATGCCATTAATTATTAAAACCGCTCACGGAAGAAACAGAGAAGAACAGAACCGAAGCGGGGCCTGAGCTTGGGTCTTCACCTCCAATGGCGTCCATGGCTGCCGCCTTCATCAAACCCTCAAACGACTTCCCCCACTCTCCAATAACCTTCATCAAAACCACCTCACCGTCGATTAAACTCCCAATTTCTCTCAGAATTAAAAACAACGCTCACCGTCGGATCCGGGTCTCGTGCGGGTTGATCGACCCGGATGGAGGCAAGCTTGTGGACCTTATTGTGGACGAATCCATGAGGGCTTTTAAGAATCGGGAGGCTCTGAGTCTGCCCCGAGTCAAGCTGTCGCGAATCGATCTGCAATGGGTGCACGTTCTGAGCGAGGGCTGGGCTAGCCCTCTTTCCGGGTTCATGAGAGAATCCGAGTTCCTCCAAACGCTTCATTTCAATTCGCTCCGTCTTCATGATGGATCGGTTGTTAACATGTCGGTGCCCATCGTTTTGGCCATCGACGATGCCGTCAAGACCCGAATCGGCGCCTCCTCTAAGGTCGCGCTCTTCGGCTCCGATGATCGCCTGGTTGCAATTCTCAATGAGTAAGATCACGCCTTTTGTTTTAACCGTTTATTTTTTTTCTTCTCATTTTATATATATATATTTTTTTAAATAAAGAGTAAATTTTTTTGGGCTTTTAAAAATATGCCTTGTATTTAATTTTTCATATAAAAACATTATATTAAAATTTTATAATTTTGCAATTCGGATGCTACTCAAGCTCGTATATATTTGTTTTAATTAATTGAGTTACATATCGAGAAATTTTATATTTGATCTACACGTCAGTTTTTGTTGAATTTTTTAAAACAAAGTCGGGACAATATTCTCTTTATCGGTGTAGGAATAGAACTGCAGCTAACACGAACCAAGGTCGTATTTTTCTTTTTTACTTGATTAGATAAAAATAGAATTTCTCGATCGACTGATTATACTAAAAAATTGTAATTTTTATAAAAGAGTTCGATTTTTTTAATTTTTTTATAATAATAATTGATAAAAAAGGATGAGTAGATATGGACATTTTAAGACGAGGAGTGTTCGTAGGTTTTTCTTAGTACAACAAGTGGGGTATGAGAATTTGAACTTCCATTTTCGAGAAGATTGAACATTTTTAAACTCAATTTAATTGTTCGGTTGTAATTTTTTTTCGAGTAGTATTGAGATATACAAGCAACCGAAGGAAGAAAGGATTGCAAGAACATGGGGAACAACAGCTCCAGGTCTTCCTTATGTTGATGAGGCTATTAAGAATGCTGGAAATTGGTTGATAGGAGGTGATCTAGAGGTCATTGAACCAATCAAGTACCACGATGGGCTCGACTCGTTTCGATTATCGCCTGCACAACTTCGCAACGAGTTCATCAGCCGCAATGCCGATGCAGTGTTCGCTTTCCAGCTTCGCAATCCGGTGCATAACGGTCATGCTCTGCTCATGAACGATACTCGTCGACGCCTTCTTGATATGGGCTACACCAACCCCATCCTTTTGCTTAATCCATTGGGTGGCTACACCAAGGCTGATGATGTTCCTCTTCATTGGAGGATCAAACAACATCAAAAGGTACCACACCACACCCCTCCTTTCATATCATGTTTCCATTTTCCTCAAATACTAACCACACTTCATTAGGTTCTTGAAGATGGTGTGCTTGATCCCGAGACGACCGTTGTCTCCATTTTCCCGTCGCCGATGCACTACGCCGGTCCAACCGAGGTGCAGTGGCACGCAAAGGCAAGGATCAATGCAGGGGCTAACTTTTACATTGTTGGTCGAGATCCTGCTGGTATGGGCCATCCTACTGAGAAGAGGGACCTTTATGATCCTGACCATGGGAAGAAAGTATTGAGCATGGCTCCTGGTTTAGAGCGCCTAAATATCCTTCCTTTCAAGGTATCATATGAACTTGCAACTTTTCTGCCACAATCACTTCATATTTCTACATATCCTTTAGTTCCAACTTCCAATTATAATGCAGGTTGCTGCATATGACAAAACTCGAGGCAAAATGGCCTTTTTTGATCCTTCAAGACCTCAGGATTTCCTCTTCATATCAGGCACCAAGGTACTTCCTCATACCAATCTCCGCAAGTCGAGAGAATCGATGTCGTTTCATAGCTTTTCCAACAGGAAATTACTGTATTTTGTCTTTAAAACTAAAAAACAAAAGAGGGGTGTGAGATCCTACATTGGTTAGAGAGAAGAACGAAGCATTTCTTATAAGGGTGTAGAAACCACTCCCTAGCAGACGCGTTTTAAAACCTTGAGGGGAAGCCAAAAAATGACATCAGAGCTAGGCACCAAACGGTGTGCCAGCAAGGATGCTGGCTCCCAAGGGCGGAGAAGAGAAAGAAACATTCCTTATAAGGGTGTGGAAATTTTTCCCTAGCAGATGCGTTTTAAAATTTTGGGGGGAAACCCAGAAGGGAAAGGAAAGCCCAAAGAGGATAATATCTGCTAGTAGTGGGCTTGGGCTGTTACAAATGGTATTAGAGCTAGGCATCGAGCGTTGTGCCAGTGAGGACGTTGGGCTCCCAAGAGGGTAATTGTGAGATCCCACATCGGTTGGAGAGGAGAACGAAGTATTCCTTATAAGGGTGTGGAAACCTCTCCCTAGCAAACGCGTTTTAAAACCTTGAGGGGAAACCTAAAAGAGAAAACTCAAATATGACAATAAAGATGACAGTATCTATCTACTATTCTGAGTCGAGGTCTCTTCTTGGTATTTGTTAGATGCGAACTCTTGCAAGGAATAAGGAGAACCCTCCAGATGGGTTTATGTGTCCCGGTGGCTGGAGCGTATTGGTCGAATACTACGAAAGCTTAGTTCCAGCAAACAATGGGAGTATCCCCAAACCTGAAGCTGTAGCAGCTTAAATCCTGTAGATCATAGTACATCACAGGATTTCAGCACAACACTTCATGCTCCTGTTTGTGATATAAACTTGACATTCTGTTAGATGTCTTGAGCTGAATAAAGTCATTTGAGGCAGTGGCCGAACCCTGTAACATATATGCTCATGGGCATGTAAAATTTAAGTGTTATGCATTTCGATTTTCATTTTTATGTAATATAAGTGTGATTCTAGCTAGCATGGTAAGTTTGAATTTCTCTTTCTGGACTGGTTGTATCTTTCTGAACTGGAAAATTGTGAGATCTCCCGTCGATTGGAGTCTTATAAGAGTGTGGAAACCTTTTCTT

mRNA sequence

AGAAAAAAAAGTACCTTTTAGTTTTGGCGCATAATAAACCGAATTGATCATGCCATTAATTATTAAAACCGCTCACGGAAGAAACAGAGAAGAACAGAACCGAAGCGGGGCCTGAGCTTGGGTCTTCACCTCCAATGGCGTCCATGGCTGCCGCCTTCATCAAACCCTCAAACGACTTCCCCCACTCTCCAATAACCTTCATCAAAACCACCTCACCGTCGATTAAACTCCCAATTTCTCTCAGAATTAAAAACAACGCTCACCGTCGGATCCGGGTCTCGTGCGGGTTGATCGACCCGGATGGAGGCAAGCTTGTGGACCTTATTGTGGACGAATCCATGAGGGCTTTTAAGAATCGGGAGGCTCTGAGTCTGCCCCGAGTCAAGCTGTCGCGAATCGATCTGCAATGGGTGCACGTTCTGAGCGAGGGCTGGGCTAGCCCTCTTTCCGGGTTCATGAGAGAATCCGAGTTCCTCCAAACGCTTCATTTCAATTCGCTCCGTCTTCATGATGGATCGGTTGTTAACATGTCGGTGCCCATCGTTTTGGCCATCGACGATGCCGTCAAGACCCGAATCGGCGCCTCCTCTAAGGTCGCGCTCTTCGGCTCCGATGATCGCCTGGTTGCAATTCTCAATGATAGTATTGAGATATACAAGCAACCGAAGGAAGAAAGGATTGCAAGAACATGGGGAACAACAGCTCCAGGTCTTCCTTATGTTGATGAGGCTATTAAGAATGCTGGAAATTGGTTGATAGGAGGTGATCTAGAGGTCATTGAACCAATCAAGTACCACGATGGGCTCGACTCGTTTCGATTATCGCCTGCACAACTTCGCAACGAGTTCATCAGCCGCAATGCCGATGCAGTGTTCGCTTTCCAGCTTCGCAATCCGGTGCATAACGGTCATGCTCTGCTCATGAACGATACTCGTCGACGCCTTCTTGATATGGGCTACACCAACCCCATCCTTTTGCTTAATCCATTGGGTGGCTACACCAAGGCTGATGATGTTCCTCTTCATTGGAGGATCAAACAACATCAAAAGGTTCTTGAAGATGGTGTGCTTGATCCCGAGACGACCGTTGTCTCCATTTTCCCGTCGCCGATGCACTACGCCGGTCCAACCGAGGTGCAGTGGCACGCAAAGGCAAGGATCAATGCAGGGGCTAACTTTTACATTGTTGGTCGAGATCCTGCTGGTATGGGCCATCCTACTGAGAAGAGGGACCTTTATGATCCTGACCATGGGAAGAAAGTATTGAGCATGGCTCCTGGTTTAGAGCGCCTAAATATCCTTCCTTTCAAGGTTGCTGCATATGACAAAACTCGAGGCAAAATGGCCTTTTTTGATCCTTCAAGACCTCAGGATTTCCTCTTCATATCAGGCACCAAGATGCGAACTCTTGCAAGGAATAAGGAGAACCCTCCAGATGGGTTTATGTGTCCCGGTGGCTGGAGCGTATTGGTCGAATACTACGAAAGCTTAGTTCCAGCAAACAATGGGAGTATCCCCAAACCTGAAGCTGTAGCAGCTTAAATCCTGTAGATCATAGTACATCACAGGATTTCAGCACAACACTTCATGCTCCTGTTTGTGATATAAACTTGACATTCTGTTAGATGTCTTGAGCTGAATAAAGTCATTTGAGGCAGTGGCCGAACCCTGTAACATATATGCTCATGGGCATGTAAAATTTAAGTGTTATGCATTTCGATTTTCATTTTTATGTAATATAAGTGTGATTCTAGCTAGCATGGTAAGTTTGAATTTCTCTTTCTGGACTGGTTGTATCTTTCTGAACTGGAAAATTGTGAGATCTCCCGTCGATTGGAGTCTTATAAGAGTGTGGAAACCTTTTCTT

Coding sequence (CDS)

ATGGCGTCCATGGCTGCCGCCTTCATCAAACCCTCAAACGACTTCCCCCACTCTCCAATAACCTTCATCAAAACCACCTCACCGTCGATTAAACTCCCAATTTCTCTCAGAATTAAAAACAACGCTCACCGTCGGATCCGGGTCTCGTGCGGGTTGATCGACCCGGATGGAGGCAAGCTTGTGGACCTTATTGTGGACGAATCCATGAGGGCTTTTAAGAATCGGGAGGCTCTGAGTCTGCCCCGAGTCAAGCTGTCGCGAATCGATCTGCAATGGGTGCACGTTCTGAGCGAGGGCTGGGCTAGCCCTCTTTCCGGGTTCATGAGAGAATCCGAGTTCCTCCAAACGCTTCATTTCAATTCGCTCCGTCTTCATGATGGATCGGTTGTTAACATGTCGGTGCCCATCGTTTTGGCCATCGACGATGCCGTCAAGACCCGAATCGGCGCCTCCTCTAAGGTCGCGCTCTTCGGCTCCGATGATCGCCTGGTTGCAATTCTCAATGATAGTATTGAGATATACAAGCAACCGAAGGAAGAAAGGATTGCAAGAACATGGGGAACAACAGCTCCAGGTCTTCCTTATGTTGATGAGGCTATTAAGAATGCTGGAAATTGGTTGATAGGAGGTGATCTAGAGGTCATTGAACCAATCAAGTACCACGATGGGCTCGACTCGTTTCGATTATCGCCTGCACAACTTCGCAACGAGTTCATCAGCCGCAATGCCGATGCAGTGTTCGCTTTCCAGCTTCGCAATCCGGTGCATAACGGTCATGCTCTGCTCATGAACGATACTCGTCGACGCCTTCTTGATATGGGCTACACCAACCCCATCCTTTTGCTTAATCCATTGGGTGGCTACACCAAGGCTGATGATGTTCCTCTTCATTGGAGGATCAAACAACATCAAAAGGTTCTTGAAGATGGTGTGCTTGATCCCGAGACGACCGTTGTCTCCATTTTCCCGTCGCCGATGCACTACGCCGGTCCAACCGAGGTGCAGTGGCACGCAAAGGCAAGGATCAATGCAGGGGCTAACTTTTACATTGTTGGTCGAGATCCTGCTGGTATGGGCCATCCTACTGAGAAGAGGGACCTTTATGATCCTGACCATGGGAAGAAAGTATTGAGCATGGCTCCTGGTTTAGAGCGCCTAAATATCCTTCCTTTCAAGGTTGCTGCATATGACAAAACTCGAGGCAAAATGGCCTTTTTTGATCCTTCAAGACCTCAGGATTTCCTCTTCATATCAGGCACCAAGATGCGAACTCTTGCAAGGAATAAGGAGAACCCTCCAGATGGGTTTATGTGTCCCGGTGGCTGGAGCGTATTGGTCGAATACTACGAAAGCTTAGTTCCAGCAAACAATGGGAGTATCCCCAAACCTGAAGCTGTAGCAGCTTAA

Protein sequence

MASMAAAFIKPSNDFPHSPITFIKTTSPSIKLPISLRIKNNAHRRIRVSCGLIDPDGGKLVDLIVDESMRAFKNREALSLPRVKLSRIDLQWVHVLSEGWASPLSGFMRESEFLQTLHFNSLRLHDGSVVNMSVPIVLAIDDAVKTRIGASSKVALFGSDDRLVAILNDSIEIYKQPKEERIARTWGTTAPGLPYVDEAIKNAGNWLIGGDLEVIEPIKYHDGLDSFRLSPAQLRNEFISRNADAVFAFQLRNPVHNGHALLMNDTRRRLLDMGYTNPILLLNPLGGYTKADDVPLHWRIKQHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLERLNILPFKVAAYDKTRGKMAFFDPSRPQDFLFISGTKMRTLARNKENPPDGFMCPGGWSVLVEYYESLVPANNGSIPKPEAVAA
BLAST of Carg10052-RA vs. NCBI nr
Match: XP_022958733.1 (ATP sulfurylase 1, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 951.0 bits (2457), Expect = 1.5e-273
Identity = 463/468 (98.93%), Postives = 465/468 (99.36%), Query Frame = 0

Query: 1   MASMAAAFIKPSNDFPHSPITFIKTTSPSIKLPISLRIKNNAHRRIRVSCGLIDPDGGKL 60
           MASMAAAFIKPSN FPHSPITFIKTTSPSIKLPISLRIKNNAHRRIRVSCGLIDPDGGKL
Sbjct: 1   MASMAAAFIKPSNAFPHSPITFIKTTSPSIKLPISLRIKNNAHRRIRVSCGLIDPDGGKL 60

Query: 61  VDLIVDESMRAFKNREALSLPRVKLSRIDLQWVHVLSEGWASPLSGFMRESEFLQTLHFN 120
           VDLIVDESMRAFKNREALSLPR+KLSRIDLQWVHVLSEGWASPLSGFMRESEFLQTLHFN
Sbjct: 61  VDLIVDESMRAFKNREALSLPRIKLSRIDLQWVHVLSEGWASPLSGFMRESEFLQTLHFN 120

Query: 121 SLRLHDGSVVNMSVPIVLAIDDAVKTRIGASSKVALFGSDDRLVAILNDSIEIYKQPKEE 180
           SLRLHDGSVVNMSVPIVLAIDDAVKTRIGASSKVALFGSDDRLVAILND IEIYKQPKEE
Sbjct: 121 SLRLHDGSVVNMSVPIVLAIDDAVKTRIGASSKVALFGSDDRLVAILND-IEIYKQPKEE 180

Query: 181 RIARTWGTTAPGLPYVDEAIKNAGNWLIGGDLEVIEPIKYHDGLDSFRLSPAQLRNEFIS 240
           RIARTWGTTAPGLPYVDEAIKNAGNWLIGGDLEVIEPIKYHDGLDSFRLSPAQLRNEFIS
Sbjct: 181 RIARTWGTTAPGLPYVDEAIKNAGNWLIGGDLEVIEPIKYHDGLDSFRLSPAQLRNEFIS 240

Query: 241 RNADAVFAFQLRNPVHNGHALLMNDTRRRLLDMGYTNPILLLNPLGGYTKADDVPLHWRI 300
           RNA AVFAFQLRNPVHNGHALLMNDTRRRLLDMGYTNPILLLNPLGGYTKADDVPLHWRI
Sbjct: 241 RNAGAVFAFQLRNPVHNGHALLMNDTRRRLLDMGYTNPILLLNPLGGYTKADDVPLHWRI 300

Query: 301 KQHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGH 360
           KQHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGH
Sbjct: 301 KQHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGH 360

Query: 361 PTEKRDLYDPDHGKKVLSMAPGLERLNILPFKVAAYDKTRGKMAFFDPSRPQDFLFISGT 420
           PTEKRDLYDPDHGKKVLSMAPGLERLNILPFKVAAYDKT+GKMAFFDPSRPQDFLFISGT
Sbjct: 361 PTEKRDLYDPDHGKKVLSMAPGLERLNILPFKVAAYDKTQGKMAFFDPSRPQDFLFISGT 420

Query: 421 KMRTLARNKENPPDGFMCPGGWSVLVEYYESLVPANNGSIPKPEAVAA 469
           KMRTLARNKENPPDGFMCPGGWSVLVEYYESLVPANNGSIPKPEAVAA
Sbjct: 421 KMRTLARNKENPPDGFMCPGGWSVLVEYYESLVPANNGSIPKPEAVAA 467

BLAST of Carg10052-RA vs. NCBI nr
Match: XP_023548667.1 (ATP sulfurylase 1, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 939.5 bits (2427), Expect = 4.4e-270
Identity = 458/468 (97.86%), Postives = 461/468 (98.50%), Query Frame = 0

Query: 1   MASMAAAFIKPSNDFPHSPITFIKTTSPSIKLPISLRIKNNAHRRIRVSCGLIDPDGGKL 60
           MASMAAAFIKPSN FPHSPITF+KTTSP I+LPISLRIKNNA RRIRVSCGLIDPDGGKL
Sbjct: 1   MASMAAAFIKPSNAFPHSPITFVKTTSPWIQLPISLRIKNNARRRIRVSCGLIDPDGGKL 60

Query: 61  VDLIVDESMRAFKNREALSLPRVKLSRIDLQWVHVLSEGWASPLSGFMRESEFLQTLHFN 120
           VDLIVDESMRAFKNREALSLPRVKLSRIDLQWVHVLSEGWASPLSGFMRESEFLQTLHFN
Sbjct: 61  VDLIVDESMRAFKNREALSLPRVKLSRIDLQWVHVLSEGWASPLSGFMRESEFLQTLHFN 120

Query: 121 SLRLHDGSVVNMSVPIVLAIDDAVKTRIGASSKVALFGSDDRLVAILNDSIEIYKQPKEE 180
           SLRLHDGSVVNMSVPIVLAIDDAVKTRIGASSKVALFGSDDR VAILND IEIYKQPKEE
Sbjct: 121 SLRLHDGSVVNMSVPIVLAIDDAVKTRIGASSKVALFGSDDRPVAILND-IEIYKQPKEE 180

Query: 181 RIARTWGTTAPGLPYVDEAIKNAGNWLIGGDLEVIEPIKYHDGLDSFRLSPAQLRNEFIS 240
           RIARTWGTTAPGLPYVDEAIKNAGNWLIGGDLEVIEPIKYHDGLDSFRLSPAQLRNEFIS
Sbjct: 181 RIARTWGTTAPGLPYVDEAIKNAGNWLIGGDLEVIEPIKYHDGLDSFRLSPAQLRNEFIS 240

Query: 241 RNADAVFAFQLRNPVHNGHALLMNDTRRRLLDMGYTNPILLLNPLGGYTKADDVPLHWRI 300
           RNADAVFAFQLRNPVHNGHALLM DTRRRLLDMGYTNPILLLNPLGGYTKADDVPLHWRI
Sbjct: 241 RNADAVFAFQLRNPVHNGHALLMTDTRRRLLDMGYTNPILLLNPLGGYTKADDVPLHWRI 300

Query: 301 KQHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGH 360
           KQHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGH
Sbjct: 301 KQHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGH 360

Query: 361 PTEKRDLYDPDHGKKVLSMAPGLERLNILPFKVAAYDKTRGKMAFFDPSRPQDFLFISGT 420
           PTEKRDLYDPDHGKKVLSMAPGLERLNILPFKVAAYDKT+GKMAFFDPSRPQDFLFISGT
Sbjct: 361 PTEKRDLYDPDHGKKVLSMAPGLERLNILPFKVAAYDKTQGKMAFFDPSRPQDFLFISGT 420

Query: 421 KMRTLARNKENPPDGFMCPGGWSVLVEYYESLVPANNGSIPKPEAVAA 469
           KMRTLARNKENPPDGFMCPGGWSVLVEYYESLVPANNG IPKPEAVAA
Sbjct: 421 KMRTLARNKENPPDGFMCPGGWSVLVEYYESLVPANNGRIPKPEAVAA 467

BLAST of Carg10052-RA vs. NCBI nr
Match: XP_023006723.1 (ATP sulfurylase 1, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 931.8 bits (2407), Expect = 9.2e-268
Identity = 453/467 (97.00%), Postives = 459/467 (98.29%), Query Frame = 0

Query: 1   MASMAAAFIKPSNDFPHSPITFIKTTSPSIKLPISLRIKNNAHRRIRVSCGLIDPDGGKL 60
           MASMAAAFIKP+N FP SPITF+KT SPSI+LPISLRI+NNA RRIRVSCGLIDPDGGKL
Sbjct: 1   MASMAAAFIKPTNAFPQSPITFVKTISPSIQLPISLRIRNNARRRIRVSCGLIDPDGGKL 60

Query: 61  VDLIVDESMRAFKNREALSLPRVKLSRIDLQWVHVLSEGWASPLSGFMRESEFLQTLHFN 120
           +DLIVDESMRAFKNREALSLPRVKLSRIDLQWVH+LSEGWASPLSGFMRESEFLQTLHFN
Sbjct: 61  MDLIVDESMRAFKNREALSLPRVKLSRIDLQWVHILSEGWASPLSGFMRESEFLQTLHFN 120

Query: 121 SLRLHDGSVVNMSVPIVLAIDDAVKTRIGASSKVALFGSDDRLVAILNDSIEIYKQPKEE 180
           SLRLHDGSVVNMSVPIVLAIDDAVKTRIGASSKVALFGSDDR VAILND IEIYKQPKEE
Sbjct: 121 SLRLHDGSVVNMSVPIVLAIDDAVKTRIGASSKVALFGSDDRPVAILND-IEIYKQPKEE 180

Query: 181 RIARTWGTTAPGLPYVDEAIKNAGNWLIGGDLEVIEPIKYHDGLDSFRLSPAQLRNEFIS 240
           RIARTWGTTAPGLPYVDEAIKNAGNWLIGGDLEVIEPIKYHDGLDSFRLSPAQLRNEFIS
Sbjct: 181 RIARTWGTTAPGLPYVDEAIKNAGNWLIGGDLEVIEPIKYHDGLDSFRLSPAQLRNEFIS 240

Query: 241 RNADAVFAFQLRNPVHNGHALLMNDTRRRLLDMGYTNPILLLNPLGGYTKADDVPLHWRI 300
           RNADAVFAFQLRNPVHNGHALLM DTRRRLLDMGYTNPILLLNPLGGYTKADDVPLHWRI
Sbjct: 241 RNADAVFAFQLRNPVHNGHALLMTDTRRRLLDMGYTNPILLLNPLGGYTKADDVPLHWRI 300

Query: 301 KQHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGH 360
           KQHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGH
Sbjct: 301 KQHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGH 360

Query: 361 PTEKRDLYDPDHGKKVLSMAPGLERLNILPFKVAAYDKTRGKMAFFDPSRPQDFLFISGT 420
           PTEKRDLYDPDHGKKVLSMAPGLERLNILPFKVAAYDKTRGKMAFFDPSRPQDFLFISGT
Sbjct: 361 PTEKRDLYDPDHGKKVLSMAPGLERLNILPFKVAAYDKTRGKMAFFDPSRPQDFLFISGT 420

Query: 421 KMRTLARNKENPPDGFMCPGGWSVLVEYYESLVPANNGSIPKPEAVA 468
           KMRTLARNKENPPDGFMCPGGWSVLVEYYESLVPAN GSIPKPEAVA
Sbjct: 421 KMRTLARNKENPPDGFMCPGGWSVLVEYYESLVPANIGSIPKPEAVA 466

BLAST of Carg10052-RA vs. NCBI nr
Match: XP_004150566.1 (PREDICTED: ATP sulfurylase 1, chloroplastic [Cucumis sativus] >KGN61658.1 hypothetical protein Csa_2G215520 [Cucumis sativus])

HSP 1 Score: 878.6 bits (2269), Expect = 9.2e-252
Identity = 427/468 (91.24%), Postives = 438/468 (93.59%), Query Frame = 0

Query: 1   MASMAAAFIKPSNDFPHSPITFIKTTSPSIKLPISLRIKNNAHRRIRVSCGLIDPDGGKL 60
           MASMAAAFIKPSN FP SPITF K  +P I LP+SLR+K   HRRIRVSCGLIDPDGGKL
Sbjct: 1   MASMAAAFIKPSNLFPQSPITFTKAVTPPIMLPMSLRVKTKTHRRIRVSCGLIDPDGGKL 60

Query: 61  VDLIVDESMRAFKNREALSLPRVKLSRIDLQWVHVLSEGWASPLSGFMRESEFLQTLHFN 120
           V+LIV+ESMR  KNREALSLPR+KLSRIDLQWVHVLSEGWASPL+GFMRESEFLQTLHFN
Sbjct: 61  VELIVEESMRGSKNREALSLPRIKLSRIDLQWVHVLSEGWASPLTGFMRESEFLQTLHFN 120

Query: 121 SLRLHDGSVVNMSVPIVLAIDDAVKTRIGASSKVALFGSDDRLVAILNDSIEIYKQPKEE 180
           SLRL DGSVVNMSVPIVLAIDD  K RIG SSKVALFGSDDR VAILND IEIYK PKEE
Sbjct: 121 SLRLQDGSVVNMSVPIVLAIDDDFKNRIGDSSKVALFGSDDRPVAILND-IEIYKHPKEE 180

Query: 181 RIARTWGTTAPGLPYVDEAIKNAGNWLIGGDLEVIEPIKYHDGLDSFRLSPAQLRNEFIS 240
           RIARTWGTTA GLPYVDEAIKNAGNWLIGGDLEVIEPIKYHDGLD FRLSPAQLRNEF S
Sbjct: 181 RIARTWGTTASGLPYVDEAIKNAGNWLIGGDLEVIEPIKYHDGLDRFRLSPAQLRNEFTS 240

Query: 241 RNADAVFAFQLRNPVHNGHALLMNDTRRRLLDMGYTNPILLLNPLGGYTKADDVPLHWRI 300
           RNADAVFAFQLRNPVHNGHALLM DTRRRLL+MGY NPILLLNPLGGYTKADDVPLHWRI
Sbjct: 241 RNADAVFAFQLRNPVHNGHALLMTDTRRRLLNMGYNNPILLLNPLGGYTKADDVPLHWRI 300

Query: 301 KQHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGH 360
           KQHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGH
Sbjct: 301 KQHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGH 360

Query: 361 PTEKRDLYDPDHGKKVLSMAPGLERLNILPFKVAAYDKTRGKMAFFDPSRPQDFLFISGT 420
           PTEKRDLYD DHGKKVLSMAPGLERLNILPFKVAAYDKT+GKMAFFDPSRPQDFLFISGT
Sbjct: 361 PTEKRDLYDADHGKKVLSMAPGLERLNILPFKVAAYDKTQGKMAFFDPSRPQDFLFISGT 420

Query: 421 KMRTLARNKENPPDGFMCPGGWSVLVEYYESLVPANNGSIPKPEAVAA 469
           KMR LARNKENPPDGFMCP GW+VLVEYY+SLVPA NG IPKPEAVAA
Sbjct: 421 KMRNLARNKENPPDGFMCPSGWNVLVEYYKSLVPAENGRIPKPEAVAA 467

BLAST of Carg10052-RA vs. NCBI nr
Match: XP_008449310.1 (PREDICTED: ATP sulfurylase 1, chloroplastic [Cucumis melo])

HSP 1 Score: 873.2 bits (2255), Expect = 3.9e-250
Identity = 425/468 (90.81%), Postives = 440/468 (94.02%), Query Frame = 0

Query: 1   MASMAAAFIKPSNDFPHSPITFIKTTSPSIKLPISLRIKNNAHRRIRVSCGLIDPDGGKL 60
           MASMAAAFIKPSN F  SPITF K  +P IKLP+SLR+K+N  RRIRVS GLIDPDGGKL
Sbjct: 1   MASMAAAFIKPSNPFSKSPITFSKAVTPPIKLPMSLRVKSNTRRRIRVSGGLIDPDGGKL 60

Query: 61  VDLIVDESMRAFKNREALSLPRVKLSRIDLQWVHVLSEGWASPLSGFMRESEFLQTLHFN 120
           V+LIV+ESMR  KNREALSLPR+KLSRID+QWVHVLSEGWASPL+GFMRESEFLQTLHFN
Sbjct: 61  VELIVEESMRGSKNREALSLPRIKLSRIDVQWVHVLSEGWASPLTGFMRESEFLQTLHFN 120

Query: 121 SLRLHDGSVVNMSVPIVLAIDDAVKTRIGASSKVALFGSDDRLVAILNDSIEIYKQPKEE 180
           SLRL DGSVVNMSVPIVLAIDD +K RIG SSKVALFGSDDR VAILND IEIYK PKEE
Sbjct: 121 SLRLQDGSVVNMSVPIVLAIDDDLKNRIGDSSKVALFGSDDRPVAILND-IEIYKHPKEE 180

Query: 181 RIARTWGTTAPGLPYVDEAIKNAGNWLIGGDLEVIEPIKYHDGLDSFRLSPAQLRNEFIS 240
           RIARTWGTTA GLPYVDEAIKNAGNWLIGGDLEVIEPIKYHDGLD FRLSPAQLRNEF S
Sbjct: 181 RIARTWGTTASGLPYVDEAIKNAGNWLIGGDLEVIEPIKYHDGLDRFRLSPAQLRNEFTS 240

Query: 241 RNADAVFAFQLRNPVHNGHALLMNDTRRRLLDMGYTNPILLLNPLGGYTKADDVPLHWRI 300
           RNADAVFAFQLRNPVHNGHALLM DTRRRLL+MGY NPILLLNPLGGYTKADDVPLHWRI
Sbjct: 241 RNADAVFAFQLRNPVHNGHALLMTDTRRRLLNMGYNNPILLLNPLGGYTKADDVPLHWRI 300

Query: 301 KQHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGH 360
           KQHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGH
Sbjct: 301 KQHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGH 360

Query: 361 PTEKRDLYDPDHGKKVLSMAPGLERLNILPFKVAAYDKTRGKMAFFDPSRPQDFLFISGT 420
           P EKRDLYD DHGKKVLSMAPGLERLNILPFKVAAYDKT+GKMAFFDPSRPQDFLFISGT
Sbjct: 361 PIEKRDLYDADHGKKVLSMAPGLERLNILPFKVAAYDKTQGKMAFFDPSRPQDFLFISGT 420

Query: 421 KMRTLARNKENPPDGFMCPGGWSVLVEYYESLVPANNGSIPKPEAVAA 469
           KMR LARNKENPPDGFMCPGGW+VLVEYY+SLVPA+NG IPKPEAVAA
Sbjct: 421 KMRNLARNKENPPDGFMCPGGWNVLVEYYKSLVPADNGRIPKPEAVAA 467

BLAST of Carg10052-RA vs. TAIR10
Match: AT3G22890.1 (ATP sulfurylase 1)

HSP 1 Score: 747.3 bits (1928), Expect = 5.8e-216
Identity = 368/465 (79.14%), Postives = 397/465 (85.38%), Query Frame = 0

Query: 1   MASMAAAFIKPSNDFPHSPITFIKTTSPSIKLP---ISLRIKNNAHRRIRVSCGLIDPDG 60
           MASMAA   K    F   P+T    +SP+  LP   +S   K+   R   +  GLI PDG
Sbjct: 1   MASMAAVLSK--TPFLSQPLT---KSSPNSDLPFAAVSFPSKSLRRRVGSIRAGLIAPDG 60

Query: 61  GKLVDLIVDESMRAFKNREALSLPRVKLSRIDLQWVHVLSEGWASPLSGFMRESEFLQTL 120
           GKLV+LIV+E  R  K  EA  LPRV+L+ IDLQW+HVLSEGWASPL GFMRESEFLQTL
Sbjct: 61  GKLVELIVEEPKRREKKHEAADLPRVELTAIDLQWMHVLSEGWASPLGGFMRESEFLQTL 120

Query: 121 HFNSLRLHDGSVVNMSVPIVLAIDDAVKTRIGASSKVALFGSDDRLVAILNDSIEIYKQP 180
           HFNSLRL DGSVVNMSVPIVLAIDD  K RIG S++VALF SD   VAIL+D IEIYK P
Sbjct: 121 HFNSLRLDDGSVVNMSVPIVLAIDDEQKARIGESTRVALFNSDGNPVAILSD-IEIYKHP 180

Query: 181 KEERIARTWGTTAPGLPYVDEAIKNAGNWLIGGDLEVIEPIKYHDGLDSFRLSPAQLRNE 240
           KEERIARTWGTTAPGLPYVDEAI NAGNWLIGGDLEV+EP+KY+DGLD FRLSPA+LR E
Sbjct: 181 KEERIARTWGTTAPGLPYVDEAITNAGNWLIGGDLEVLEPVKYNDGLDRFRLSPAELRKE 240

Query: 241 FISRNADAVFAFQLRNPVHNGHALLMNDTRRRLLDMGYTNPILLLNPLGGYTKADDVPLH 300
              RNADAVFAFQLRNPVHNGHALLM DTRRRLL+MGY NPILLL+PLGG+TKADDVPL 
Sbjct: 241 LEKRNADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPILLLHPLGGFTKADDVPLD 300

Query: 301 WRIKQHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAG 360
           WR+KQH+KVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAG
Sbjct: 301 WRMKQHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAG 360

Query: 361 MGHPTEKRDLYDPDHGKKVLSMAPGLERLNILPFKVAAYDKTRGKMAFFDPSRPQDFLFI 420
           MGHP EKRDLYD DHGKKVLSMAPGLERLNILPF+VAAYDKT+GKMAFFDPSRPQDFLFI
Sbjct: 361 MGHPVEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRPQDFLFI 420

Query: 421 SGTKMRTLARNKENPPDGFMCPGGWSVLVEYYESLVPANNGSIPK 463
           SGTKMRTLA+N ENPPDGFMCPGGW VLV+YYESL PA NG +P+
Sbjct: 421 SGTKMRTLAKNNENPPDGFMCPGGWKVLVDYYESLTPAGNGRLPE 459

BLAST of Carg10052-RA vs. TAIR10
Match: AT4G14680.1 (Pseudouridine synthase/archaeosine transglycosylase-like family protein)

HSP 1 Score: 719.9 bits (1857), Expect = 9.9e-208
Identity = 352/463 (76.03%), Postives = 387/463 (83.59%), Query Frame = 0

Query: 1   MASMAAAFIKPSNDFPHSPIT-FIKTTSPSIKLPISLRIKNNAHRRIRVSCGLIDPDGGK 60
           MASM+  F KP++ F   P+T   K+ S +  +      K  + R I V  GLI+PDGGK
Sbjct: 1   MASMSTVFPKPTS-FISQPLTKSHKSDSVTTSISFPSNSKTRSLRTISVRAGLIEPDGGK 60

Query: 61  LVDLIVDESMRAFKNREALSLPRVKLSRIDLQWVHVLSEGWASPLSGFMRESEFLQTLHF 120
           LVDL+V E  R  K  EA  LPRV+L+ IDLQW+HVLSEGWASPL GFMRESEFLQTLHF
Sbjct: 61  LVDLVVPEPRRREKKHEAADLPRVRLTAIDLQWMHVLSEGWASPLRGFMRESEFLQTLHF 120

Query: 121 NSLRLHDGSVVNMSVPIVLAIDDAVKTRIGASSKVALFGSDDRLVAILNDSIEIYKQPKE 180
           N L L DGSVVNMSVPIVLAIDD  K  IG S +V+L  SDD  +AILND IEIYK PKE
Sbjct: 121 NLLNLDDGSVVNMSVPIVLAIDDQQKALIGESKRVSLVDSDDNPIAILND-IEIYKHPKE 180

Query: 181 ERIARTWGTTAPGLPYVDEAIKNAGNWLIGGDLEVIEPIKYHDGLDSFRLSPAQLRNEFI 240
           ERIARTWGTTAPGLPYV+EAI NAG+WLIGGDLEV+EP+KY+DGLD FRLSP +LR E  
Sbjct: 181 ERIARTWGTTAPGLPYVEEAITNAGDWLIGGDLEVLEPVKYNDGLDRFRLSPFELRKELE 240

Query: 241 SRNADAVFAFQLRNPVHNGHALLMNDTRRRLLDMGYTNPILLLNPLGGYTKADDVPLHWR 300
            R ADAVFAFQLRNPVHNGHALLM DTRRRLL+MGY NPILLL+PLGG+TKADDVPL WR
Sbjct: 241 KRGADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPILLLHPLGGFTKADDVPLSWR 300

Query: 301 IKQHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMG 360
           +KQH+KVLEDGVLDPETTVVSIFPSPM YAGPTEVQWHAKARINAGANFYIVGRDPAGMG
Sbjct: 301 MKQHEKVLEDGVLDPETTVVSIFPSPMLYAGPTEVQWHAKARINAGANFYIVGRDPAGMG 360

Query: 361 HPTEKRDLYDPDHGKKVLSMAPGLERLNILPFKVAAYDKTRGKMAFFDPSRPQDFLFISG 420
           HP EKRDLYD DHGKKVLSMAPGLERLNILPF+VAAYDKT+GKMAFFDPSR QDFLFISG
Sbjct: 361 HPVEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRAQDFLFISG 420

Query: 421 TKMRTLARNKENPPDGFMCPGGWSVLVEYYESLVPANNGSIPK 463
           TKMR LA+N+ENPPDGFMCPGGW VLV+YY+SL    N  +P+
Sbjct: 421 TKMRALAKNRENPPDGFMCPGGWKVLVDYYDSLTLTGNTKLPE 461

BLAST of Carg10052-RA vs. TAIR10
Match: AT5G43780.1 (Pseudouridine synthase/archaeosine transglycosylase-like family protein)

HSP 1 Score: 702.2 bits (1811), Expect = 2.1e-202
Identity = 353/473 (74.63%), Postives = 392/473 (82.88%), Query Frame = 0

Query: 1   MASMAAAFIKPSNDFPHSPITFIKTTSPSIKLPISLRI----KNNAHRRIRVSCGLIDPD 60
           MAS AAA +  S  F  SP+  I     S   P S+ +    +  + R + V  GLI+PD
Sbjct: 1   MASSAAAIVSGS-PFRSSPL--IHNHHASRYAPGSISVVSLPRQVSRRGLSVKSGLIEPD 60

Query: 61  GGKLVDLIVDESMRAFKNREALSLP-RVKLSRIDLQWVHVLSEGWASPLSGFMRESEFLQ 120
           GGKL++L+V+ES R     EA ++P R+KL+R+DL+WVHVLSEGWASPL GFMR+SEFLQ
Sbjct: 61  GGKLMNLVVEESRRRVMKHEAETVPARIKLNRVDLEWVHVLSEGWASPLKGFMRQSEFLQ 120

Query: 121 TLHFNSLRLHDGSVVNMSVPIVLAIDDAVKTRIGASSKVALFGSDDRLVAILNDSIEIYK 180
           TLHFNS RL DGSVVNMSVPIVLAIDD  K RIG S++V L  S    +AILND IEIYK
Sbjct: 121 TLHFNSFRLEDGSVVNMSVPIVLAIDDDQKFRIGDSNQVTLVDSVGNPIAILND-IEIYK 180

Query: 181 QPKEERIARTWGTTAPGLPYVDEAIKNAGNWLIGGDLEVIEPIKYHDGLDSFRLSPAQLR 240
            PKEERIARTWGTTA GLPY +EAI  AGNWLIGGDL+V+EPIKY+DGLD FRLSP+QLR
Sbjct: 181 HPKEERIARTWGTTARGLPYAEEAITKAGNWLIGGDLQVLEPIKYNDGLDRFRLSPSQLR 240

Query: 241 NEFISRNADAVFAFQLRNPVHNGHALLMNDTRRRLLDMGYTNPILLLNPLGGYTKADDVP 300
            EFI R ADAVFAFQLRNPVHNGHALLM DTRRRLL+MGY NP+LLLNPLGG+TKADDVP
Sbjct: 241 EEFIRRGADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPVLLLNPLGGFTKADDVP 300

Query: 301 LHWRIKQHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDP 360
           L WR++QH+KVLEDGVLDPETTVVSIFPSPM YAGPTEVQWHAKARINAGANFYIVGRDP
Sbjct: 301 LSWRMRQHEKVLEDGVLDPETTVVSIFPSPMLYAGPTEVQWHAKARINAGANFYIVGRDP 360

Query: 361 AGMGHPTEKRDLYDPDHGKKVLSMAPGLERLNILPFKVAAYDKTRGKMAFFDPSRPQDFL 420
           AGMGHPTEKRDLYD DHGKKVLSMAPGLERLNILPFKVAAYDKT+GKMAFFDPSR QDFL
Sbjct: 361 AGMGHPTEKRDLYDADHGKKVLSMAPGLERLNILPFKVAAYDKTQGKMAFFDPSRSQDFL 420

Query: 421 FISGTKMRTLARNKENPPDGFMCPGGWSVLVEYYESLVPANNGSIPKPEAVAA 469
           FISGTKMR LA+ KENPPDGFMCP GW VLV+YY+SL  A  G+    EAVA+
Sbjct: 421 FISGTKMRGLAKKKENPPDGFMCPSGWKVLVDYYDSL-SAETGNGRVSEAVAS 468

BLAST of Carg10052-RA vs. TAIR10
Match: AT1G19920.1 (Pseudouridine synthase/archaeosine transglycosylase-like family protein)

HSP 1 Score: 657.9 bits (1696), Expect = 4.6e-189
Identity = 316/448 (70.54%), Postives = 370/448 (82.59%), Query Frame = 0

Query: 10  KPSNDFPHS---PITFIKTT--SPSIKLPISLRIKNNAHRRIRVSCGLIDPDGGKLVDLI 69
           +P N  P S    I+F+ ++  +P + L     +   +  ++ V   LIDPDGG+LV+LI
Sbjct: 17  QPRNSKPSSFTNQISFLSSSNNNPFLNLVYKRNLTMQSVSKMTVKSSLIDPDGGELVELI 76

Query: 70  VDESMRAFKNREALSLPRVKLSRIDLQWVHVLSEGWASPLSGFMRESEFLQTLHFNSLRL 129
           V E+    K  E+ ++P+VKL++IDL+WVHV+SEGWASPL GFMRE E+LQ+LHFNSLRL
Sbjct: 77  VPETEIGVKKAESETMPKVKLNQIDLEWVHVISEGWASPLKGFMREDEYLQSLHFNSLRL 136

Query: 130 HDGSVVNMSVPIVLAIDDAVKTRIGASSKVALFGSDDRLVAILNDSIEIYKQPKEERIAR 189
            +G+ VNMS+PIVLAIDD  K +IG+S  VAL      ++  L  S+EIYK  KEERIAR
Sbjct: 137 KNGTFVNMSLPIVLAIDDDTKEQIGSSENVALVCPQGDIIGSLR-SVEIYKHNKEERIAR 196

Query: 190 TWGTTAPGLPYVDEAIKNAGNWLIGGDLEVIEPIKYHDGLDSFRLSPAQLRNEFISRNAD 249
           TWGTT+PGLPYV+E I  +GNWLIGGDLEV EPIKY+DGLD +RLSP QLR EF +R AD
Sbjct: 197 TWGTTSPGLPYVEEYITPSGNWLIGGDLEVFEPIKYNDGLDHYRLSPKQLREEFDNRQAD 256

Query: 250 AVFAFQLRNPVHNGHALLMNDTRRRLLDMGYTNPILLLNPLGGYTKADDVPLHWRIKQHQ 309
           AVFAFQLRNPVHNGHALLMNDTR+RLL+MGY NP+LLL+PLGG+TKADDVPL  R++QH 
Sbjct: 257 AVFAFQLRNPVHNGHALLMNDTRKRLLEMGYKNPVLLLHPLGGFTKADDVPLDVRMEQHS 316

Query: 310 KVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHPTEK 369
           KVLEDGVLDP+TT+VSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHPTEK
Sbjct: 317 KVLEDGVLDPKTTIVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHPTEK 376

Query: 370 RDLYDPDHGKKVLSMAPGLERLNILPFKVAAYDKTRGKMAFFDPSRPQDFLFISGTKMRT 429
           RDLYDPDHGK+VLSMAPGLE+LNILPF+VAAYD    KMAFFDPSR ++FLFISGTKMRT
Sbjct: 377 RDLYDPDHGKRVLSMAPGLEKLNILPFRVAAYDTIEKKMAFFDPSRAKEFLFISGTKMRT 436

Query: 430 LARNKENPPDGFMCPGGWSVLVEYYESL 453
            AR  ENPPDGFMCP GW+VLV+YYESL
Sbjct: 437 YARTGENPPDGFMCPSGWNVLVKYYESL 463

BLAST of Carg10052-RA vs. Swiss-Prot
Match: sp|Q9LIK9|APS1_ARATH (ATP sulfurylase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APS1 PE=1 SV=1)

HSP 1 Score: 747.3 bits (1928), Expect = 1.0e-214
Identity = 368/465 (79.14%), Postives = 397/465 (85.38%), Query Frame = 0

Query: 1   MASMAAAFIKPSNDFPHSPITFIKTTSPSIKLP---ISLRIKNNAHRRIRVSCGLIDPDG 60
           MASMAA   K    F   P+T    +SP+  LP   +S   K+   R   +  GLI PDG
Sbjct: 1   MASMAAVLSK--TPFLSQPLT---KSSPNSDLPFAAVSFPSKSLRRRVGSIRAGLIAPDG 60

Query: 61  GKLVDLIVDESMRAFKNREALSLPRVKLSRIDLQWVHVLSEGWASPLSGFMRESEFLQTL 120
           GKLV+LIV+E  R  K  EA  LPRV+L+ IDLQW+HVLSEGWASPL GFMRESEFLQTL
Sbjct: 61  GKLVELIVEEPKRREKKHEAADLPRVELTAIDLQWMHVLSEGWASPLGGFMRESEFLQTL 120

Query: 121 HFNSLRLHDGSVVNMSVPIVLAIDDAVKTRIGASSKVALFGSDDRLVAILNDSIEIYKQP 180
           HFNSLRL DGSVVNMSVPIVLAIDD  K RIG S++VALF SD   VAIL+D IEIYK P
Sbjct: 121 HFNSLRLDDGSVVNMSVPIVLAIDDEQKARIGESTRVALFNSDGNPVAILSD-IEIYKHP 180

Query: 181 KEERIARTWGTTAPGLPYVDEAIKNAGNWLIGGDLEVIEPIKYHDGLDSFRLSPAQLRNE 240
           KEERIARTWGTTAPGLPYVDEAI NAGNWLIGGDLEV+EP+KY+DGLD FRLSPA+LR E
Sbjct: 181 KEERIARTWGTTAPGLPYVDEAITNAGNWLIGGDLEVLEPVKYNDGLDRFRLSPAELRKE 240

Query: 241 FISRNADAVFAFQLRNPVHNGHALLMNDTRRRLLDMGYTNPILLLNPLGGYTKADDVPLH 300
              RNADAVFAFQLRNPVHNGHALLM DTRRRLL+MGY NPILLL+PLGG+TKADDVPL 
Sbjct: 241 LEKRNADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPILLLHPLGGFTKADDVPLD 300

Query: 301 WRIKQHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAG 360
           WR+KQH+KVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAG
Sbjct: 301 WRMKQHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAG 360

Query: 361 MGHPTEKRDLYDPDHGKKVLSMAPGLERLNILPFKVAAYDKTRGKMAFFDPSRPQDFLFI 420
           MGHP EKRDLYD DHGKKVLSMAPGLERLNILPF+VAAYDKT+GKMAFFDPSRPQDFLFI
Sbjct: 361 MGHPVEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRPQDFLFI 420

Query: 421 SGTKMRTLARNKENPPDGFMCPGGWSVLVEYYESLVPANNGSIPK 463
           SGTKMRTLA+N ENPPDGFMCPGGW VLV+YYESL PA NG +P+
Sbjct: 421 SGTKMRTLAKNNENPPDGFMCPGGWKVLVDYYESLTPAGNGRLPE 459

BLAST of Carg10052-RA vs. Swiss-Prot
Match: sp|O23324|APS3_ARATH (ATP-sulfurylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APS3 PE=1 SV=1)

HSP 1 Score: 719.9 bits (1857), Expect = 1.8e-206
Identity = 352/463 (76.03%), Postives = 387/463 (83.59%), Query Frame = 0

Query: 1   MASMAAAFIKPSNDFPHSPIT-FIKTTSPSIKLPISLRIKNNAHRRIRVSCGLIDPDGGK 60
           MASM+  F KP++ F   P+T   K+ S +  +      K  + R I V  GLI+PDGGK
Sbjct: 1   MASMSTVFPKPTS-FISQPLTKSHKSDSVTTSISFPSNSKTRSLRTISVRAGLIEPDGGK 60

Query: 61  LVDLIVDESMRAFKNREALSLPRVKLSRIDLQWVHVLSEGWASPLSGFMRESEFLQTLHF 120
           LVDL+V E  R  K  EA  LPRV+L+ IDLQW+HVLSEGWASPL GFMRESEFLQTLHF
Sbjct: 61  LVDLVVPEPRRREKKHEAADLPRVRLTAIDLQWMHVLSEGWASPLRGFMRESEFLQTLHF 120

Query: 121 NSLRLHDGSVVNMSVPIVLAIDDAVKTRIGASSKVALFGSDDRLVAILNDSIEIYKQPKE 180
           N L L DGSVVNMSVPIVLAIDD  K  IG S +V+L  SDD  +AILND IEIYK PKE
Sbjct: 121 NLLNLDDGSVVNMSVPIVLAIDDQQKALIGESKRVSLVDSDDNPIAILND-IEIYKHPKE 180

Query: 181 ERIARTWGTTAPGLPYVDEAIKNAGNWLIGGDLEVIEPIKYHDGLDSFRLSPAQLRNEFI 240
           ERIARTWGTTAPGLPYV+EAI NAG+WLIGGDLEV+EP+KY+DGLD FRLSP +LR E  
Sbjct: 181 ERIARTWGTTAPGLPYVEEAITNAGDWLIGGDLEVLEPVKYNDGLDRFRLSPFELRKELE 240

Query: 241 SRNADAVFAFQLRNPVHNGHALLMNDTRRRLLDMGYTNPILLLNPLGGYTKADDVPLHWR 300
            R ADAVFAFQLRNPVHNGHALLM DTRRRLL+MGY NPILLL+PLGG+TKADDVPL WR
Sbjct: 241 KRGADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPILLLHPLGGFTKADDVPLSWR 300

Query: 301 IKQHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMG 360
           +KQH+KVLEDGVLDPETTVVSIFPSPM YAGPTEVQWHAKARINAGANFYIVGRDPAGMG
Sbjct: 301 MKQHEKVLEDGVLDPETTVVSIFPSPMLYAGPTEVQWHAKARINAGANFYIVGRDPAGMG 360

Query: 361 HPTEKRDLYDPDHGKKVLSMAPGLERLNILPFKVAAYDKTRGKMAFFDPSRPQDFLFISG 420
           HP EKRDLYD DHGKKVLSMAPGLERLNILPF+VAAYDKT+GKMAFFDPSR QDFLFISG
Sbjct: 361 HPVEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRAQDFLFISG 420

Query: 421 TKMRTLARNKENPPDGFMCPGGWSVLVEYYESLVPANNGSIPK 463
           TKMR LA+N+ENPPDGFMCPGGW VLV+YY+SL    N  +P+
Sbjct: 421 TKMRALAKNRENPPDGFMCPGGWKVLVDYYDSLTLTGNTKLPE 461

BLAST of Carg10052-RA vs. Swiss-Prot
Match: sp|Q9S7D8|APS4_ARATH (ATP sulfurylase 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APS4 PE=2 SV=1)

HSP 1 Score: 702.2 bits (1811), Expect = 3.9e-201
Identity = 353/473 (74.63%), Postives = 392/473 (82.88%), Query Frame = 0

Query: 1   MASMAAAFIKPSNDFPHSPITFIKTTSPSIKLPISLRI----KNNAHRRIRVSCGLIDPD 60
           MAS AAA +  S  F  SP+  I     S   P S+ +    +  + R + V  GLI+PD
Sbjct: 1   MASSAAAIVSGS-PFRSSPL--IHNHHASRYAPGSISVVSLPRQVSRRGLSVKSGLIEPD 60

Query: 61  GGKLVDLIVDESMRAFKNREALSLP-RVKLSRIDLQWVHVLSEGWASPLSGFMRESEFLQ 120
           GGKL++L+V+ES R     EA ++P R+KL+R+DL+WVHVLSEGWASPL GFMR+SEFLQ
Sbjct: 61  GGKLMNLVVEESRRRVMKHEAETVPARIKLNRVDLEWVHVLSEGWASPLKGFMRQSEFLQ 120

Query: 121 TLHFNSLRLHDGSVVNMSVPIVLAIDDAVKTRIGASSKVALFGSDDRLVAILNDSIEIYK 180
           TLHFNS RL DGSVVNMSVPIVLAIDD  K RIG S++V L  S    +AILND IEIYK
Sbjct: 121 TLHFNSFRLEDGSVVNMSVPIVLAIDDDQKFRIGDSNQVTLVDSVGNPIAILND-IEIYK 180

Query: 181 QPKEERIARTWGTTAPGLPYVDEAIKNAGNWLIGGDLEVIEPIKYHDGLDSFRLSPAQLR 240
            PKEERIARTWGTTA GLPY +EAI  AGNWLIGGDL+V+EPIKY+DGLD FRLSP+QLR
Sbjct: 181 HPKEERIARTWGTTARGLPYAEEAITKAGNWLIGGDLQVLEPIKYNDGLDRFRLSPSQLR 240

Query: 241 NEFISRNADAVFAFQLRNPVHNGHALLMNDTRRRLLDMGYTNPILLLNPLGGYTKADDVP 300
            EFI R ADAVFAFQLRNPVHNGHALLM DTRRRLL+MGY NP+LLLNPLGG+TKADDVP
Sbjct: 241 EEFIRRGADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPVLLLNPLGGFTKADDVP 300

Query: 301 LHWRIKQHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDP 360
           L WR++QH+KVLEDGVLDPETTVVSIFPSPM YAGPTEVQWHAKARINAGANFYIVGRDP
Sbjct: 301 LSWRMRQHEKVLEDGVLDPETTVVSIFPSPMLYAGPTEVQWHAKARINAGANFYIVGRDP 360

Query: 361 AGMGHPTEKRDLYDPDHGKKVLSMAPGLERLNILPFKVAAYDKTRGKMAFFDPSRPQDFL 420
           AGMGHPTEKRDLYD DHGKKVLSMAPGLERLNILPFKVAAYDKT+GKMAFFDPSR QDFL
Sbjct: 361 AGMGHPTEKRDLYDADHGKKVLSMAPGLERLNILPFKVAAYDKTQGKMAFFDPSRSQDFL 420

Query: 421 FISGTKMRTLARNKENPPDGFMCPGGWSVLVEYYESLVPANNGSIPKPEAVAA 469
           FISGTKMR LA+ KENPPDGFMCP GW VLV+YY+SL  A  G+    EAVA+
Sbjct: 421 FISGTKMRGLAKKKENPPDGFMCPSGWKVLVDYYDSL-SAETGNGRVSEAVAS 468

BLAST of Carg10052-RA vs. Swiss-Prot
Match: sp|Q43870|APS2_ARATH (ATP sulfurylase 2 OS=Arabidopsis thaliana OX=3702 GN=APS2 PE=1 SV=1)

HSP 1 Score: 657.9 bits (1696), Expect = 8.4e-188
Identity = 316/448 (70.54%), Postives = 370/448 (82.59%), Query Frame = 0

Query: 10  KPSNDFPHS---PITFIKTT--SPSIKLPISLRIKNNAHRRIRVSCGLIDPDGGKLVDLI 69
           +P N  P S    I+F+ ++  +P + L     +   +  ++ V   LIDPDGG+LV+LI
Sbjct: 17  QPRNSKPSSFTNQISFLSSSNNNPFLNLVYKRNLTMQSVSKMTVKSSLIDPDGGELVELI 76

Query: 70  VDESMRAFKNREALSLPRVKLSRIDLQWVHVLSEGWASPLSGFMRESEFLQTLHFNSLRL 129
           V E+    K  E+ ++P+VKL++IDL+WVHV+SEGWASPL GFMRE E+LQ+LHFNSLRL
Sbjct: 77  VPETEIGVKKAESETMPKVKLNQIDLEWVHVISEGWASPLKGFMREDEYLQSLHFNSLRL 136

Query: 130 HDGSVVNMSVPIVLAIDDAVKTRIGASSKVALFGSDDRLVAILNDSIEIYKQPKEERIAR 189
            +G+ VNMS+PIVLAIDD  K +IG+S  VAL      ++  L  S+EIYK  KEERIAR
Sbjct: 137 KNGTFVNMSLPIVLAIDDDTKEQIGSSENVALVCPQGDIIGSLR-SVEIYKHNKEERIAR 196

Query: 190 TWGTTAPGLPYVDEAIKNAGNWLIGGDLEVIEPIKYHDGLDSFRLSPAQLRNEFISRNAD 249
           TWGTT+PGLPYV+E I  +GNWLIGGDLEV EPIKY+DGLD +RLSP QLR EF +R AD
Sbjct: 197 TWGTTSPGLPYVEEYITPSGNWLIGGDLEVFEPIKYNDGLDHYRLSPKQLREEFDNRQAD 256

Query: 250 AVFAFQLRNPVHNGHALLMNDTRRRLLDMGYTNPILLLNPLGGYTKADDVPLHWRIKQHQ 309
           AVFAFQLRNPVHNGHALLMNDTR+RLL+MGY NP+LLL+PLGG+TKADDVPL  R++QH 
Sbjct: 257 AVFAFQLRNPVHNGHALLMNDTRKRLLEMGYKNPVLLLHPLGGFTKADDVPLDVRMEQHS 316

Query: 310 KVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHPTEK 369
           KVLEDGVLDP+TT+VSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHPTEK
Sbjct: 317 KVLEDGVLDPKTTIVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHPTEK 376

Query: 370 RDLYDPDHGKKVLSMAPGLERLNILPFKVAAYDKTRGKMAFFDPSRPQDFLFISGTKMRT 429
           RDLYDPDHGK+VLSMAPGLE+LNILPF+VAAYD    KMAFFDPSR ++FLFISGTKMRT
Sbjct: 377 RDLYDPDHGKRVLSMAPGLEKLNILPFRVAAYDTIEKKMAFFDPSRAKEFLFISGTKMRT 436

Query: 430 LARNKENPPDGFMCPGGWSVLVEYYESL 453
            AR  ENPPDGFMCP GW+VLV+YYESL
Sbjct: 437 YARTGENPPDGFMCPSGWNVLVKYYESL 463

BLAST of Carg10052-RA vs. Swiss-Prot
Match: sp|O95340|PAPS2_HUMAN (Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 2 OS=Homo sapiens OX=9606 GN=PAPSS2 PE=1 SV=2)

HSP 1 Score: 495.7 bits (1275), Expect = 5.5e-139
Identity = 236/391 (60.36%), Postives = 299/391 (76.47%), Query Frame = 0

Query: 62  DLIVDESMRAFKNREALSLPRVKLSRIDLQWVHVLSEGWASPLSGFMRESEFLQTLHFNS 121
           +L V E+       EA +LP + ++++DLQWV VLSEGWA+PL GFMRE E+LQ +HF++
Sbjct: 226 ELFVPENKLDHVRAEAETLPSLSITKLDLQWVQVLSEGWATPLKGFMREKEYLQVMHFDT 285

Query: 122 LRLHDGSVVNMSVPIVLAIDDAVKTRIGASSKVALFGSDDRLVAILNDSIEIYKQPKEER 181
           L L DG V+NMS+PIVL +    KTR+   SK  L     R VAIL D+ E Y+  KEER
Sbjct: 286 L-LDDG-VINMSIPIVLPVSAEDKTRLEGCSKFVL-AHGGRRVAILRDA-EFYEHRKEER 345

Query: 182 IARTWGTTAPGLPYVDEAIKNAGNWLIGGDLEVIEPIKYHDGLDSFRLSPAQLRNEFISR 241
            +R WGTT    P++ + +  +G+WL+GGDL+V+E I+++DGLD +RL+P +L+ +    
Sbjct: 346 CSRVWGTTCTKHPHI-KMVMESGDWLVGGDLQVLEKIRWNDGLDQYRLTPLELKQKCKEM 405

Query: 242 NADAVFAFQLRNPVHNGHALLMNDTRRRLLDMGYTNPILLLNPLGGYTKADDVPLHWRIK 301
           NADAVFAFQLRNPVHNGHALLM DTRRRLL+ GY +P+LLL+PLGG+TK DDVPL WR+K
Sbjct: 406 NADAVFAFQLRNPVHNGHALLMQDTRRRLLERGYKHPVLLLHPLGGWTKDDDVPLDWRMK 465

Query: 302 QHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP 361
           QH  VLE+GVLDP++T+V+IFPSPM YAGPTEVQWH ++R+ AGANFYIVGRDPAGM HP
Sbjct: 466 QHAAVLEEGVLDPKSTIVAIFPSPMLYAGPTEVQWHCRSRMIAGANFYIVGRDPAGMPHP 525

Query: 362 TEKRDLYDPDHGKKVLSMAPGLERLNILPFKVAAYDKTRGKMAFFDPSRPQDFLFISGTK 421
             K+DLY+P HG KVLSMAPGL  + I+PF+VAAY+K +  M F+DP+R  +F FISGT+
Sbjct: 526 ETKKDLYEPTHGGKVLSMAPGLTSVEIIPFRVAAYNKAKKAMDFYDPARHNEFDFISGTR 585

Query: 422 MRTLARNKENPPDGFMCPGGWSVLVEYYESL 453
           MR LAR  ENPPDGFM P  W VL +YY SL
Sbjct: 586 MRKLAREGENPPDGFMAPKAWKVLTDYYRSL 611

BLAST of Carg10052-RA vs. TrEMBL
Match: tr|A0A0A0LIM7|A0A0A0LIM7_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G215520 PE=4 SV=1)

HSP 1 Score: 878.6 bits (2269), Expect = 6.1e-252
Identity = 427/468 (91.24%), Postives = 438/468 (93.59%), Query Frame = 0

Query: 1   MASMAAAFIKPSNDFPHSPITFIKTTSPSIKLPISLRIKNNAHRRIRVSCGLIDPDGGKL 60
           MASMAAAFIKPSN FP SPITF K  +P I LP+SLR+K   HRRIRVSCGLIDPDGGKL
Sbjct: 1   MASMAAAFIKPSNLFPQSPITFTKAVTPPIMLPMSLRVKTKTHRRIRVSCGLIDPDGGKL 60

Query: 61  VDLIVDESMRAFKNREALSLPRVKLSRIDLQWVHVLSEGWASPLSGFMRESEFLQTLHFN 120
           V+LIV+ESMR  KNREALSLPR+KLSRIDLQWVHVLSEGWASPL+GFMRESEFLQTLHFN
Sbjct: 61  VELIVEESMRGSKNREALSLPRIKLSRIDLQWVHVLSEGWASPLTGFMRESEFLQTLHFN 120

Query: 121 SLRLHDGSVVNMSVPIVLAIDDAVKTRIGASSKVALFGSDDRLVAILNDSIEIYKQPKEE 180
           SLRL DGSVVNMSVPIVLAIDD  K RIG SSKVALFGSDDR VAILND IEIYK PKEE
Sbjct: 121 SLRLQDGSVVNMSVPIVLAIDDDFKNRIGDSSKVALFGSDDRPVAILND-IEIYKHPKEE 180

Query: 181 RIARTWGTTAPGLPYVDEAIKNAGNWLIGGDLEVIEPIKYHDGLDSFRLSPAQLRNEFIS 240
           RIARTWGTTA GLPYVDEAIKNAGNWLIGGDLEVIEPIKYHDGLD FRLSPAQLRNEF S
Sbjct: 181 RIARTWGTTASGLPYVDEAIKNAGNWLIGGDLEVIEPIKYHDGLDRFRLSPAQLRNEFTS 240

Query: 241 RNADAVFAFQLRNPVHNGHALLMNDTRRRLLDMGYTNPILLLNPLGGYTKADDVPLHWRI 300
           RNADAVFAFQLRNPVHNGHALLM DTRRRLL+MGY NPILLLNPLGGYTKADDVPLHWRI
Sbjct: 241 RNADAVFAFQLRNPVHNGHALLMTDTRRRLLNMGYNNPILLLNPLGGYTKADDVPLHWRI 300

Query: 301 KQHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGH 360
           KQHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGH
Sbjct: 301 KQHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGH 360

Query: 361 PTEKRDLYDPDHGKKVLSMAPGLERLNILPFKVAAYDKTRGKMAFFDPSRPQDFLFISGT 420
           PTEKRDLYD DHGKKVLSMAPGLERLNILPFKVAAYDKT+GKMAFFDPSRPQDFLFISGT
Sbjct: 361 PTEKRDLYDADHGKKVLSMAPGLERLNILPFKVAAYDKTQGKMAFFDPSRPQDFLFISGT 420

Query: 421 KMRTLARNKENPPDGFMCPGGWSVLVEYYESLVPANNGSIPKPEAVAA 469
           KMR LARNKENPPDGFMCP GW+VLVEYY+SLVPA NG IPKPEAVAA
Sbjct: 421 KMRNLARNKENPPDGFMCPSGWNVLVEYYKSLVPAENGRIPKPEAVAA 467

BLAST of Carg10052-RA vs. TrEMBL
Match: tr|A0A1S3BMN3|A0A1S3BMN3_CUCME (ATP sulfurylase 1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103491225 PE=4 SV=1)

HSP 1 Score: 873.2 bits (2255), Expect = 2.6e-250
Identity = 425/468 (90.81%), Postives = 440/468 (94.02%), Query Frame = 0

Query: 1   MASMAAAFIKPSNDFPHSPITFIKTTSPSIKLPISLRIKNNAHRRIRVSCGLIDPDGGKL 60
           MASMAAAFIKPSN F  SPITF K  +P IKLP+SLR+K+N  RRIRVS GLIDPDGGKL
Sbjct: 1   MASMAAAFIKPSNPFSKSPITFSKAVTPPIKLPMSLRVKSNTRRRIRVSGGLIDPDGGKL 60

Query: 61  VDLIVDESMRAFKNREALSLPRVKLSRIDLQWVHVLSEGWASPLSGFMRESEFLQTLHFN 120
           V+LIV+ESMR  KNREALSLPR+KLSRID+QWVHVLSEGWASPL+GFMRESEFLQTLHFN
Sbjct: 61  VELIVEESMRGSKNREALSLPRIKLSRIDVQWVHVLSEGWASPLTGFMRESEFLQTLHFN 120

Query: 121 SLRLHDGSVVNMSVPIVLAIDDAVKTRIGASSKVALFGSDDRLVAILNDSIEIYKQPKEE 180
           SLRL DGSVVNMSVPIVLAIDD +K RIG SSKVALFGSDDR VAILND IEIYK PKEE
Sbjct: 121 SLRLQDGSVVNMSVPIVLAIDDDLKNRIGDSSKVALFGSDDRPVAILND-IEIYKHPKEE 180

Query: 181 RIARTWGTTAPGLPYVDEAIKNAGNWLIGGDLEVIEPIKYHDGLDSFRLSPAQLRNEFIS 240
           RIARTWGTTA GLPYVDEAIKNAGNWLIGGDLEVIEPIKYHDGLD FRLSPAQLRNEF S
Sbjct: 181 RIARTWGTTASGLPYVDEAIKNAGNWLIGGDLEVIEPIKYHDGLDRFRLSPAQLRNEFTS 240

Query: 241 RNADAVFAFQLRNPVHNGHALLMNDTRRRLLDMGYTNPILLLNPLGGYTKADDVPLHWRI 300
           RNADAVFAFQLRNPVHNGHALLM DTRRRLL+MGY NPILLLNPLGGYTKADDVPLHWRI
Sbjct: 241 RNADAVFAFQLRNPVHNGHALLMTDTRRRLLNMGYNNPILLLNPLGGYTKADDVPLHWRI 300

Query: 301 KQHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGH 360
           KQHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGH
Sbjct: 301 KQHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGH 360

Query: 361 PTEKRDLYDPDHGKKVLSMAPGLERLNILPFKVAAYDKTRGKMAFFDPSRPQDFLFISGT 420
           P EKRDLYD DHGKKVLSMAPGLERLNILPFKVAAYDKT+GKMAFFDPSRPQDFLFISGT
Sbjct: 361 PIEKRDLYDADHGKKVLSMAPGLERLNILPFKVAAYDKTQGKMAFFDPSRPQDFLFISGT 420

Query: 421 KMRTLARNKENPPDGFMCPGGWSVLVEYYESLVPANNGSIPKPEAVAA 469
           KMR LARNKENPPDGFMCPGGW+VLVEYY+SLVPA+NG IPKPEAVAA
Sbjct: 421 KMRNLARNKENPPDGFMCPGGWNVLVEYYKSLVPADNGRIPKPEAVAA 467

BLAST of Carg10052-RA vs. TrEMBL
Match: tr|Q1W2K0|Q1W2K0_CAMSI (ATP-sulfurylase OS=Camellia sinensis OX=4442 PE=2 SV=1)

HSP 1 Score: 770.4 bits (1988), Expect = 2.3e-219
Identity = 376/466 (80.69%), Postives = 412/466 (88.41%), Query Frame = 0

Query: 1   MASMAAAFIKPSNDFPHSPITFIKT-TSPSIKLPISLRIKNNAHRRIRVSCGLIDPDGGK 60
           MASMAA FIK     P+  ++F KT  S  +KLP+SL   N    +IR+SCGLI+PDGGK
Sbjct: 1   MASMAAFFIKT----PYQSLSFPKTHFSTPLKLPLSLH-SNPKTTKIRISCGLINPDGGK 60

Query: 61  LVDLIVDESMRAFKNREALSLPRVKLSRIDLQWVHVLSEGWASPLSGFMRESEFLQTLHF 120
           LV+L+V+ES R  K R+ALS+PR+KLSRIDLQWVHVLSEGWASPL GFMRESEFLQTLHF
Sbjct: 61  LVELVVEESQRDEKKRQALSMPRIKLSRIDLQWVHVLSEGWASPLRGFMRESEFLQTLHF 120

Query: 121 NSLRLHDGSVVNMSVPIVLAIDDAVKTRIGASSKVALFGSDDRLVAILNDSIEIYKQPKE 180
           N LRL DGSVVNMSVPIVLAI D+ K+RIG SS VAL  S D  VAIL+ +IEIYK  KE
Sbjct: 121 NLLRLDDGSVVNMSVPIVLAITDSQKSRIGESSAVALVDSGDNPVAILS-NIEIYKHNKE 180

Query: 181 ERIARTWGTTAPGLPYVDEAIKNAGNWLIGGDLEVIEPIKYHDGLDSFRLSPAQLRNEFI 240
           ERIARTWGTTAPGLPYV+EAI N+GNWLIGGDLEVIEPIKYHDGLD FRLSPA+LR EF 
Sbjct: 181 ERIARTWGTTAPGLPYVEEAITNSGNWLIGGDLEVIEPIKYHDGLDRFRLSPAELREEFT 240

Query: 241 SRNADAVFAFQLRNPVHNGHALLMNDTRRRLLDMGYTNPILLLNPLGGYTKADDVPLHWR 300
            RNADAVFAFQLRNPVHNGHALLM DTRRRLL+MGY NP+LLL+PLGGYTKADDVPL WR
Sbjct: 241 RRNADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPVLLLHPLGGYTKADDVPLSWR 300

Query: 301 IKQHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMG 360
           +KQH+KVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGM 
Sbjct: 301 MKQHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMS 360

Query: 361 HPTEKRDLYDPDHGKKVLSMAPGLERLNILPFKVAAYDKTRGKMAFFDPSRPQDFLFISG 420
           HP EKRDLYD DHGKKVLSMAPGL RLNILPFKVAAYD+T+GKMAFFDP+RPQDF+FISG
Sbjct: 361 HPVEKRDLYDADHGKKVLSMAPGLGRLNILPFKVAAYDRTQGKMAFFDPARPQDFVFISG 420

Query: 421 TKMRTLARNKENPPDGFMCPGGWSVLVEYYESLVPANNGSIPKPEA 466
           TKMRTLA+NKENPPDGFMCPGGW VLVEYY+SLVP++NG +P+P A
Sbjct: 421 TKMRTLAKNKENPPDGFMCPGGWEVLVEYYDSLVPSDNGKVPQPVA 460

BLAST of Carg10052-RA vs. TrEMBL
Match: tr|A0A2P5FW98|A0A2P5FW98_9ROSA (Sulfate adenylyltransferase, prokaryotic-type OS=Trema orientalis OX=63057 GN=TorRG33x02_018540 PE=4 SV=1)

HSP 1 Score: 770.0 bits (1987), Expect = 3.1e-219
Identity = 381/470 (81.06%), Postives = 408/470 (86.81%), Query Frame = 0

Query: 4   MAAAFIKPSNDFPHSPITFIKTTSPSIKLPISLRIKNNA----HRRIRVSCGLIDPDGGK 63
           MA  F K     P  P TF    +PS+K+ +  R  + A     +R+RVSCGLI+PDGGK
Sbjct: 1   MATLFAKTPYQSPSLPKTFNTHFAPSLKVSLPGRSGSRAGVRVGQRLRVSCGLIEPDGGK 60

Query: 64  LVDLIVDESMRAFKNREALSLPRVKLSRIDLQWVHVLSEGWASPLSGFMRESEFLQTLHF 123
           LV+L V+ES+R+ K RE L LPR++LSRIDLQWVHVLSEGWASPL GFMRESEFLQTLHF
Sbjct: 61  LVELFVEESLRSEKKREGLGLPRIQLSRIDLQWVHVLSEGWASPLRGFMRESEFLQTLHF 120

Query: 124 NSLRLHDGSVVNMSVPIVLAIDDAVKTRIGASSKVALFGSDDRLVAILNDSIEIYKQPKE 183
           NSLRL DGS VNMSVPIVLAID++ K RIG S++VAL  SDD LVAIL D IEIYK  KE
Sbjct: 121 NSLRLDDGSAVNMSVPIVLAIDESQKRRIGESTRVALVDSDDHLVAILYD-IEIYKHHKE 180

Query: 184 ERIARTWGTTAPGLPYVDEAIKNAGNWLIGGDLEVIEPIKYHDGLDSFRLSPAQLRNEFI 243
           ERIARTWGTTAP LPYVD+AI NAGNWLIGGDLEV+EPIKYHDGLD FRLSPA+LR EF 
Sbjct: 181 ERIARTWGTTAPALPYVDQAITNAGNWLIGGDLEVLEPIKYHDGLDRFRLSPAELREEFT 240

Query: 244 SRNADAVFAFQLRNPVHNGHALLMNDTRRRLLDMGYTNPILLLNPLGGYTKADDVPLHWR 303
            RNADAVFAFQLRNPVHNGHALLM DTRRRLL+MGY NPILLL+PLGGYTKADDVPL WR
Sbjct: 241 KRNADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPILLLHPLGGYTKADDVPLSWR 300

Query: 304 IKQHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMG 363
           +KQH+KVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMG
Sbjct: 301 MKQHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMG 360

Query: 364 HPTEKRDLYDPDHGKKVLSMAPGLERLNILPFKVAAYDKTRGKMAFFDPSRPQDFLFISG 423
           HP EKRDLYD DHGKKVLSMAPGLERLNILPFKVAAYDKTRGKMAFFDPSRPQDFLFISG
Sbjct: 361 HPIEKRDLYDADHGKKVLSMAPGLERLNILPFKVAAYDKTRGKMAFFDPSRPQDFLFISG 420

Query: 424 TKMRTLARNKENPPDGFMCPGGWSVLVEYYESL-VPANNGSIPKPEAVAA 469
           TKMRTLARNKENPP+GFMCPGGW VLVEYY+SL VPA NG I  PEAV A
Sbjct: 421 TKMRTLARNKENPPEGFMCPGGWQVLVEYYDSLVVPAENGKI-IPEAVPA 468

BLAST of Carg10052-RA vs. TrEMBL
Match: tr|A0A1S3AWG3|A0A1S3AWG3_CUCME (ATP sulfurylase 1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103483665 PE=4 SV=1)

HSP 1 Score: 770.0 bits (1987), Expect = 3.1e-219
Identity = 371/468 (79.27%), Postives = 406/468 (86.75%), Query Frame = 0

Query: 1   MASMAAAFIKPSNDFPHSPITFIKTTSPSIKLPISLRIKNNAHRRIRVSCGLIDPDGGKL 60
           MASMA  F K  + F     T        IK+ I+       H R+RVS GLI+PDGG+L
Sbjct: 1   MASMATRFTKTPSPFHSIQRTSYTHLGAPIKVSIATSKSKKTHLRLRVSAGLIEPDGGRL 60

Query: 61  VDLIVDESMRAFKNREALSLPRVKLSRIDLQWVHVLSEGWASPLSGFMRESEFLQTLHFN 120
           V+L+V+E +R  K +EALSLPR++LS ID+QWVHVLSEGWASPL+GFMRESEFLQTLHFN
Sbjct: 61  VELLVEEPLRGLKKKEALSLPRIELSSIDIQWVHVLSEGWASPLTGFMRESEFLQTLHFN 120

Query: 121 SLRLHDGSVVNMSVPIVLAIDDAVKTRIGASSKVALFGSDDRLVAILNDSIEIYKQPKEE 180
           SLRL DGSV NMSVPIVLAIDDA K  IG S+ VALF S +  +A+L D IEIYK PKEE
Sbjct: 121 SLRLPDGSVANMSVPIVLAIDDAQKHSIGDSTSVALFDSKNNPIAVLKD-IEIYKHPKEE 180

Query: 181 RIARTWGTTAPGLPYVDEAIKNAGNWLIGGDLEVIEPIKYHDGLDSFRLSPAQLRNEFIS 240
           RIARTWGTTAPGLPYVD++I NAGNWLIGGDLEVIEPIKYHDGLD FR SPA+LR EF  
Sbjct: 181 RIARTWGTTAPGLPYVDQSITNAGNWLIGGDLEVIEPIKYHDGLDRFRQSPAELREEFTR 240

Query: 241 RNADAVFAFQLRNPVHNGHALLMNDTRRRLLDMGYTNPILLLNPLGGYTKADDVPLHWRI 300
           RNADAVFAFQLRNPVHNGHALLM DTRRRLL+MGY NPILLL+PLGGYTKADDVPL WR+
Sbjct: 241 RNADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPILLLHPLGGYTKADDVPLSWRM 300

Query: 301 KQHQKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGH 360
           KQH+KVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGH
Sbjct: 301 KQHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGH 360

Query: 361 PTEKRDLYDPDHGKKVLSMAPGLERLNILPFKVAAYDKTRGKMAFFDPSRPQDFLFISGT 420
           PTEKRDLYD DHGKKVLSMAPGLERLNILPF+VAAYDKT+GKMAFFDPSR QDFLFISGT
Sbjct: 361 PTEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRAQDFLFISGT 420

Query: 421 KMRTLARNKENPPDGFMCPGGWSVLVEYYESLVPANNGSIPKPEAVAA 469
           KMRTLA+NKENPP+GFMCPGGW VLVEYY+SLVPA++G +P+PEAV A
Sbjct: 421 KMRTLAKNKENPPEGFMCPGGWKVLVEYYDSLVPASSGRLPEPEAVLA 467

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022958733.11.5e-27398.93ATP sulfurylase 1, chloroplastic-like [Cucurbita moschata][more]
XP_023548667.14.4e-27097.86ATP sulfurylase 1, chloroplastic-like [Cucurbita pepo subsp. pepo][more]
XP_023006723.19.2e-26897.00ATP sulfurylase 1, chloroplastic-like [Cucurbita maxima][more]
XP_004150566.19.2e-25291.24PREDICTED: ATP sulfurylase 1, chloroplastic [Cucumis sativus] >KGN61658.1 hypoth... [more]
XP_008449310.13.9e-25090.81PREDICTED: ATP sulfurylase 1, chloroplastic [Cucumis melo][more]
Match NameE-valueIdentityDescription
AT3G22890.15.8e-21679.14ATP sulfurylase 1[more]
AT4G14680.19.9e-20876.03Pseudouridine synthase/archaeosine transglycosylase-like family protein[more]
AT5G43780.12.1e-20274.63Pseudouridine synthase/archaeosine transglycosylase-like family protein[more]
AT1G19920.14.6e-18970.54Pseudouridine synthase/archaeosine transglycosylase-like family protein[more]
Match NameE-valueIdentityDescription
sp|Q9LIK9|APS1_ARATH1.0e-21479.14ATP sulfurylase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APS1 PE=1 SV... [more]
sp|O23324|APS3_ARATH1.8e-20676.03ATP-sulfurylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APS3 PE=1 SV... [more]
sp|Q9S7D8|APS4_ARATH3.9e-20174.63ATP sulfurylase 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APS4 PE=2 SV... [more]
sp|Q43870|APS2_ARATH8.4e-18870.54ATP sulfurylase 2 OS=Arabidopsis thaliana OX=3702 GN=APS2 PE=1 SV=1[more]
sp|O95340|PAPS2_HUMAN5.5e-13960.36Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 2 OS=Homo sapiens OX... [more]
Match NameE-valueIdentityDescription
tr|A0A0A0LIM7|A0A0A0LIM7_CUCSA6.1e-25291.24Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G215520 PE=4 SV=1[more]
tr|A0A1S3BMN3|A0A1S3BMN3_CUCME2.6e-25090.81ATP sulfurylase 1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103491225 PE=4 SV... [more]
tr|Q1W2K0|Q1W2K0_CAMSI2.3e-21980.69ATP-sulfurylase OS=Camellia sinensis OX=4442 PE=2 SV=1[more]
tr|A0A2P5FW98|A0A2P5FW98_9ROSA3.1e-21981.06Sulfate adenylyltransferase, prokaryotic-type OS=Trema orientalis OX=63057 GN=To... [more]
tr|A0A1S3AWG3|A0A1S3AWG3_CUCME3.1e-21979.27ATP sulfurylase 1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103483665 PE=4 SV... [more]
The following terms have been associated with this mRNA:
Vocabulary: Biological Process
TermDefinition
GO:0000103sulfate assimilation
Vocabulary: Molecular Function
TermDefinition
GO:0004781sulfate adenylyltransferase (ATP) activity
Vocabulary: INTERPRO
TermDefinition
IPR015947PUA-like_sf
IPR002650Sulphate_adenylyltransferase
IPR025980ATP-Sase_PUA-like_dom
IPR014729Rossmann-like_a/b/a_fold
IPR024951Sulfurylase_cat_dom
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000103 sulfate assimilation
biological_process GO:0006144 purine nucleobase metabolic process
cellular_component GO:0005575 cellular_component
molecular_function GO:0004781 sulfate adenylyltransferase (ATP) activity

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Carg10052Carg10052gene


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Carg10052-RACarg10052-RA-proteinpolypeptide


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg10052-RA.exon.1Carg10052-RA.exon.1exon
Carg10052-RA.exon.2Carg10052-RA.exon.2exon
Carg10052-RA.exon.3Carg10052-RA.exon.3exon
Carg10052-RA.exon.4Carg10052-RA.exon.4exon
Carg10052-RA.exon.5Carg10052-RA.exon.5exon


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg10052-RA.five_prime_UTR.1Carg10052-RA.five_prime_UTR.1five_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg10052-RA.CDS.1Carg10052-RA.CDS.1CDS
Carg10052-RA.CDS.2Carg10052-RA.CDS.2CDS
Carg10052-RA.CDS.3Carg10052-RA.CDS.3CDS
Carg10052-RA.CDS.4Carg10052-RA.CDS.4CDS
Carg10052-RA.CDS.5Carg10052-RA.CDS.5CDS


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg10052-RA.three_prime_UTR.1Carg10052-RA.three_prime_UTR.1three_prime_UTR


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR024951Sulphate adenylyltransferase catalytic domainPFAMPF01747ATP-sulfurylasecoord: 226..447
e-value: 4.0E-64
score: 215.9
NoneNo IPR availableGENE3DG3DSA:3.10.400.10coord: 52..220
e-value: 4.7E-61
score: 207.0
NoneNo IPR availablePANTHERPTHR11055:SF18ATP SULFURYLASE 1, CHLOROPLASTIC-RELATEDcoord: 25..458
NoneNo IPR availablePANTHERPTHR11055ADENYLSULFATE KINASE/SULFATE ADENYLTRANSFERASEcoord: 25..458
NoneNo IPR availableSUPERFAMILYSSF52374Nucleotidylyl transferasecoord: 226..449
IPR014729Rossmann-like alpha/beta/alpha sandwich foldGENE3DG3DSA:3.40.50.620coord: 221..455
e-value: 2.5E-99
score: 333.4
IPR025980ATP-sulfurylase PUA-like domainPFAMPF14306PUA_2coord: 53..216
e-value: 2.3E-41
score: 141.0
IPR002650Sulphate adenylyltransferaseTIGRFAMTIGR00339TIGR00339coord: 55..447
e-value: 3.3E-132
score: 438.9
IPR002650Sulphate adenylyltransferaseCDDcd00517ATPScoord: 80..448
e-value: 1.81141E-159
score: 457.865
IPR015947PUA-like superfamilySUPERFAMILYSSF88697PUA domain-likecoord: 55..220