Carg09324-RA (mRNA) Silver-seed gourd

NameCarg09324-RA
TypemRNA
OrganismCucurbita argyrosperma (Silver-seed gourd)
Descriptionacyl-protein thioesterase 2-like
LocationCucurbita_argyrosperma_scaffold_018 : 610972 .. 614438 (+)
Sequence length951
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
GTGTGAAGGGACGCGAAGGCAAAGCTTTTACAAAAGAGACAGAAACTGGAAAAGCCGATATGCTTTTCGGGTCGTTAAACACTTCTATCCCAAATTTTTTGTAATTGGTAAATAAAAACCCTCAAATCCTCTTCACTTTCTGCTTCTGTTCTTCATATTTAAGTAAATTTTAAGCAATCTGTCCAGATCTTTCCTATGAATTCTCGCCGTCATCAATGCTTGCTTGAACATTCAACTTTTAGGTCTGTAATTTGATGGCTGTCTTATCATTCTTTATGTATTGTATTTTATACCCTTTTTTTTCTTTCTTTTTTTTTTTTTTTTTTTTTTTGCATAAAAAATGGTTGAAAATTTTGATTAGTTGCTATATTCTGGTGTTGCTGTAGTTGAAGTGCTTTAGCCTTGTGGGTTGTCCTGCGTTCACGGGCATAGATTCTTTGCCTCTCGCTCTCTTGCCCTCGTAATCCCTCTTTTTGTTGCTCCCTCTGTGGCATTATGAACTGAAGGGCTATGTCAGCTCAGAGCTCGTTTTCTGCATCGATGCTTTAATCTCTTGTGGATTCTTCTTTGCCCATAAAGATTTCTTGCCTTTTTCTTCCTTTTTTGAGATGCCTTTCACTTTTTTTAGTATTTGAGGACTTTGGAGGCTGTTTATGGGTTGTGATATCAGGAGTAGCCAATGGAAAATTCATGGGTTTTAGTTCCATTTCAGCCCTCCCAAGTGCATATAAATACAGTTGGTCAATTCTAAAACTCTTCATTGCAGGAACTAATTCCAACGCCCAAGTTGTTGCCTGTCTTGCTTCCAACATTTTTGTGGAAATACAATGAGCTATAACAGCTCTGCTATGGGTTCTGGTTTGTTCACTTTCTGCTCATTTTGTTTTATTGGTTTACTGGCCATCAAGCTAAACTAGCTAGCTTTCATTTTTGCTCCATTATGTCAGGTAGTAGAACAGGCAGGATGAGCTTTGAGTTTGGGAGGACCCATGTGGTCAGGCCTAAAGGGAAGCATCAGGCAACTATTGTTTGGTTACATGGCCTTGGTGATAAGGGGTCAAGGTACTAATGGCTTATCTTCTTTACGTTCTCCTTCCTGTTTAGCCAGCTTATTAGAGTTATTTTGCTAGTTTCTGGTTGACTTACTTCGATTGAGATTAAGAAGAAACTTTGAGGTACTACGTGAGATCCCACATCAGTAGGAGAGGGGAACGAAACATTGCTCATAAGGGTGTGGAAACCTCTCCCTACCAAAGGGGCTCAGAAGGGAAAGCCCAAAGAAGACAATATCTGCTAGTGGTGAGTTTGGGCTGTTACAAATGGTATCAAAGCCAGACACCGGGCGGTGTGCTAGTGAGGACGCTAGGTCTCCAAGGGGGTGGATTGTGAGATCCCACATCGGTTGGAGAGGAGAATGAAACATTACTTATAAGGGTGTGGAAACCTCTCTCTACCAGACGCGTTTTAAAACCTTAAAGACAAACCTGTAAAGGAAAGTCCAAAGAGGACAATAACGGCCCGCCGTGGGTTTGGGCTATTACATACTTGAGTATAAACACTCAATGAACTCTATGGCGCATTACATGTAACTTGCAAACTTTCCATCCTAATTAGTGGGTTATATCTGAGCAGAGGACTTTCTTTTTGTCTTCCATGCCAAAAGTAAGGGAAGAAGTGGCTGAGTGAGAAAATGTCAATGTCTTGCCCTATCTTCTTGTGTATGTTCATATATAGAAAGTTTTAAAAATTCTATATGCCTTTACTAGAGATTCTTATTCTGATTTCTTTTTAAGTGGGAAGGTAACGTCATGTTTTGTGTCCTTTATAATTAGAAAAAAAATCAAAATCAATATAAAATAATGCTCATTATCATATGCTAGCTGATATCAGACATTTACATCATTAAAGGAGGGCTGGTTTCTTCATTATTGGAAGCCTATCTGGACATCTAATTTATTATTTATTTACTTCTAAATTTTTTCAGTTGGTCTCAGCTCTTGGAAACTCTTCCTCTACCAAACGTAAGGCTACTTAACATAGTAACCTTGATTGTCATTAAGTTTTGGGATTTGTTTAATATGATCAATTAACATAATAATTACGAATTCTGTTGCAGATTAAGTGGATTTGTCCTACTGCTCCTACTCGTCCTGTGACGTTATTTGGTGGATTTCCTTGCACTGCATGTAAGACTATCCAAATTTCATAAGTAGATTTATTAAAATTATGAGCCAAATTCCTCGTATGAAAACGATATATTTATTCTTGGAATTAGTTTAAATTATGAGTTACTCTGCCGTTTCGTATCAGGGTTTGATGTGGGAGATATTTCAGAGGAGTCTCCTGATGATTTAGAAGGCTTGGATGCTGCAGCATCGCATGTTGCTAATCTTTTGTCTACAGAACCTGCTGATAGTATGTTGTTTCTTCTTGAAAAGCTTTTAAACTCCCCCCAGCTGACTTGCTGTTTGTCATTTAATCACTGAACTTAATCATTGGTGTGATACTCCTCTGGTTGGTCACGTATTAGATTACCCGTTATTGTTGTGTCAAGGACTCGAACCGGTAGTTCTCATTTAAAACTATTTCATATATACGGTTTTGAAACGAAGAACAATTATGAATTGTATGTAGGGTAGGTATAGATAGAGGTTTCATCTACCTTCTCATATCGTTTTTACAGATGATACTTGGTGTTTGGCATACAAATTTGTCATATTCTCACTCTTTTTCTTACTAACAGTTAAACTGGGTATTGGAGGCTTCAGTATGGGTGCTGCAACTGCTATTTACTCTGCGTCACGCCGTATTCTTGGGCAATATGGAAATGGAAACCTATACCCTATTAACCTTAGTGCAGTTGTTGGGCTTAGTGGCTGGCTTCCCTGTTCAAGGTTTGATTTTTGTTTGTGAATTATGAAGAAGCCGAAACACACAGTGCACGGTCGTGAGAAAGCCTACACGGGTGGCCTTTGTTCTTTATCAAAGCCTTTTTTTGTTGTAGATCTTTGAGGAACCAGGTCAGTGTGTCACACGAAGCTGCAAGGCGCGCTGCGTCCCTGCCCATTTTACTCTGTCATGGTTCAGGTACACTGCTAGTTTACGAGTTCAAGCATTAATGCACTCTTGTGAGAACTAATTTCTCTCAATTCCCTTAATCCTATTGAATTTTACTGAGCGGTTGGTTACAGGTGACGATGTCGTAGCTTACAAACACGGGGAGAAATCTGCACATACATTAAGTTCATCTGGATTTAGCAATCTAACATTTAAATCCTATAACGGGTATGTTCGTATCTCGACTTTCTAGTGCTTCTCGTTTTGTATGTCTGATAATAAGTAACAAATAAGATGGGATCTTGCAATGCAGGCTGGGCCATTACACCATTCCTGAGGAGATGGATGTTGTTTGTAATTGGCTGACAGTCGTTTTGGGGCTCGATGGCTTACGGTTGGTCGATCACTGA

mRNA sequence

GTGTGAAGGGACGCGAAGGCAAAGCTTTTACAAAAGAGACAGAAACTGGAAAAGCCGATATGCTTTTCGGGTCGTTAAACACTTCTATCCCAAATTTTTTGTAATTGGAACTAATTCCAACGCCCAAGTTGTTGCCTGTCTTGCTTCCAACATTTTTGTGGAAATACAATGAGCTATAACAGCTCTGCTATGGGTTCTGGTAGTAGAACAGGCAGGATGAGCTTTGAGTTTGGGAGGACCCATGTGGTCAGGCCTAAAGGGAAGCATCAGGCAACTATTGTTTGGTTACATGGCCTTGGTGATAAGGGGTCAAGTTGGTCTCAGCTCTTGGAAACTCTTCCTCTACCAAACATTAAGTGGATTTGTCCTACTGCTCCTACTCGTCCTGTGACGTTATTTGGTGGATTTCCTTGCACTGCATGGTTTGATGTGGGAGATATTTCAGAGGAGTCTCCTGATGATTTAGAAGGCTTGGATGCTGCAGCATCGCATGTTGCTAATCTTTTGTCTACAGAACCTGCTGATATTAAACTGGGTATTGGAGGCTTCAGTATGGGTGCTGCAACTGCTATTTACTCTGCGTCACGCCGTATTCTTGGGCAATATGGAAATGGAAACCTATACCCTATTAACCTTAGTGCAGTTGTTGGGCTTAGTGGCTGGCTTCCCTGTTCAAGATCTTTGAGGAACCAGGTCAGTGTGTCACACGAAGCTGCAAGGCGCGCTGCGTCCCTGCCCATTTTACTCTGTCATGGTTCAGGTGACGATGTCGTAGCTTACAAACACGGGGAGAAATCTGCACATACATTAAGTTCATCTGGATTTAGCAATCTAACATTTAAATCCTATAACGGGCTGGGCCATTACACCATTCCTGAGGAGATGGATGTTGTTTGTAATTGGCTGACAGTCGTTTTGGGGCTCGATGGCTTACGGTTGGTCGATCACTGA

Coding sequence (CDS)

ATGAGCTATAACAGCTCTGCTATGGGTTCTGGTAGTAGAACAGGCAGGATGAGCTTTGAGTTTGGGAGGACCCATGTGGTCAGGCCTAAAGGGAAGCATCAGGCAACTATTGTTTGGTTACATGGCCTTGGTGATAAGGGGTCAAGTTGGTCTCAGCTCTTGGAAACTCTTCCTCTACCAAACATTAAGTGGATTTGTCCTACTGCTCCTACTCGTCCTGTGACGTTATTTGGTGGATTTCCTTGCACTGCATGGTTTGATGTGGGAGATATTTCAGAGGAGTCTCCTGATGATTTAGAAGGCTTGGATGCTGCAGCATCGCATGTTGCTAATCTTTTGTCTACAGAACCTGCTGATATTAAACTGGGTATTGGAGGCTTCAGTATGGGTGCTGCAACTGCTATTTACTCTGCGTCACGCCGTATTCTTGGGCAATATGGAAATGGAAACCTATACCCTATTAACCTTAGTGCAGTTGTTGGGCTTAGTGGCTGGCTTCCCTGTTCAAGATCTTTGAGGAACCAGGTCAGTGTGTCACACGAAGCTGCAAGGCGCGCTGCGTCCCTGCCCATTTTACTCTGTCATGGTTCAGGTGACGATGTCGTAGCTTACAAACACGGGGAGAAATCTGCACATACATTAAGTTCATCTGGATTTAGCAATCTAACATTTAAATCCTATAACGGGCTGGGCCATTACACCATTCCTGAGGAGATGGATGTTGTTTGTAATTGGCTGACAGTCGTTTTGGGGCTCGATGGCTTACGGTTGGTCGATCACTGA

Protein sequence

MSYNSSAMGSGSRTGRMSFEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLPNIKWICPTAPTRPVTLFGGFPCTAWFDVGDISEESPDDLEGLDAAASHVANLLSTEPADIKLGIGGFSMGAATAIYSASRRILGQYGNGNLYPINLSAVVGLSGWLPCSRSLRNQVSVSHEAARRAASLPILLCHGSGDDVVAYKHGEKSAHTLSSSGFSNLTFKSYNGLGHYTIPEEMDVVCNWLTVVLGLDGLRLVDH
BLAST of Carg09324-RA vs. NCBI nr
Match: XP_022961761.1 (acyl-protein thioesterase 2-like [Cucurbita moschata])

HSP 1 Score: 533.5 bits (1373), Expect = 4.0e-148
Identity = 259/260 (99.62%), Postives = 259/260 (99.62%), Query Frame = 0

Query: 1   MSYNSSAMGSGSRTGRMSFEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLP 60
           MSYNSSAMGSGSRTGRMSFEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLP
Sbjct: 1   MSYNSSAMGSGSRTGRMSFEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLP 60

Query: 61  NIKWICPTAPTRPVTLFGGFPCTAWFDVGDISEESPDDLEGLDAAASHVANLLSTEPADI 120
           NIKWICPTAPTRPVTLFGGFPCTAWFDVGDISEESPDDLEGLDAAASHVANLLSTEPADI
Sbjct: 61  NIKWICPTAPTRPVTLFGGFPCTAWFDVGDISEESPDDLEGLDAAASHVANLLSTEPADI 120

Query: 121 KLGIGGFSMGAATAIYSASRRILGQYGNGNLYPINLSAVVGLSGWLPCSRSLRNQVSVSH 180
           KLGIGGFSMGAATAIYSAS RILGQYGNGNLYPINLSAVVGLSGWLPCSRSLRNQVSVSH
Sbjct: 121 KLGIGGFSMGAATAIYSASCRILGQYGNGNLYPINLSAVVGLSGWLPCSRSLRNQVSVSH 180

Query: 181 EAARRAASLPILLCHGSGDDVVAYKHGEKSAHTLSSSGFSNLTFKSYNGLGHYTIPEEMD 240
           EAARRAASLPILLCHGSGDDVVAYKHGEKSAHTLSSSGFSNLTFKSYNGLGHYTIPEEMD
Sbjct: 181 EAARRAASLPILLCHGSGDDVVAYKHGEKSAHTLSSSGFSNLTFKSYNGLGHYTIPEEMD 240

Query: 241 VVCNWLTVVLGLDGLRLVDH 261
           VVCNWLTVVLGLDGLRLVDH
Sbjct: 241 VVCNWLTVVLGLDGLRLVDH 260

BLAST of Carg09324-RA vs. NCBI nr
Match: XP_022996560.1 (acyl-protein thioesterase 2-like [Cucurbita maxima] >XP_022996561.1 acyl-protein thioesterase 2-like [Cucurbita maxima] >XP_022996562.1 acyl-protein thioesterase 2-like [Cucurbita maxima] >XP_022996563.1 acyl-protein thioesterase 2-like [Cucurbita maxima])

HSP 1 Score: 528.5 bits (1360), Expect = 1.3e-146
Identity = 255/260 (98.08%), Postives = 257/260 (98.85%), Query Frame = 0

Query: 1   MSYNSSAMGSGSRTGRMSFEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLP 60
           MSYNSS MGSGSRTGRMSFEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLP
Sbjct: 1   MSYNSSTMGSGSRTGRMSFEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLP 60

Query: 61  NIKWICPTAPTRPVTLFGGFPCTAWFDVGDISEESPDDLEGLDAAASHVANLLSTEPADI 120
           NIKWICPTAPTRPVTLFGGFPCTAWFDVGDISEESPDDLEGLDAAASHVANLLSTEPADI
Sbjct: 61  NIKWICPTAPTRPVTLFGGFPCTAWFDVGDISEESPDDLEGLDAAASHVANLLSTEPADI 120

Query: 121 KLGIGGFSMGAATAIYSASRRILGQYGNGNLYPINLSAVVGLSGWLPCSRSLRNQVSVSH 180
           KLGIGGFSMGAATAIYSAS RILGQYGNGNLYPINLSAVVGLSGWLPCSRSLRNQVSVSH
Sbjct: 121 KLGIGGFSMGAATAIYSASCRILGQYGNGNLYPINLSAVVGLSGWLPCSRSLRNQVSVSH 180

Query: 181 EAARRAASLPILLCHGSGDDVVAYKHGEKSAHTLSSSGFSNLTFKSYNGLGHYTIPEEMD 240
           EAARRAASLPILLCHGSGDDVVAYKHGEKSAHTLSSSGFSNLTFKSYNGLGHYTIPEEMD
Sbjct: 181 EAARRAASLPILLCHGSGDDVVAYKHGEKSAHTLSSSGFSNLTFKSYNGLGHYTIPEEMD 240

Query: 241 VVCNWLTVVLGLDGLRLVDH 261
           VVCNWLTV+L LDG+RLVDH
Sbjct: 241 VVCNWLTVILALDGIRLVDH 260

BLAST of Carg09324-RA vs. NCBI nr
Match: XP_023545446.1 (acyl-protein thioesterase 2-like [Cucurbita pepo subsp. pepo] >XP_023545447.1 acyl-protein thioesterase 2-like [Cucurbita pepo subsp. pepo] >XP_023545448.1 acyl-protein thioesterase 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 525.4 bits (1352), Expect = 1.1e-145
Identity = 254/260 (97.69%), Postives = 256/260 (98.46%), Query Frame = 0

Query: 1   MSYNSSAMGSGSRTGRMSFEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLP 60
           MSYNSS MGSGSRTGRMSFEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLP
Sbjct: 1   MSYNSSTMGSGSRTGRMSFEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLP 60

Query: 61  NIKWICPTAPTRPVTLFGGFPCTAWFDVGDISEESPDDLEGLDAAASHVANLLSTEPADI 120
           NIKWICPTAPTRPVTLFGGFPCTAWFDVGDISEESPDDLEGLDAAASHVANLLSTEPADI
Sbjct: 61  NIKWICPTAPTRPVTLFGGFPCTAWFDVGDISEESPDDLEGLDAAASHVANLLSTEPADI 120

Query: 121 KLGIGGFSMGAATAIYSASRRILGQYGNGNLYPINLSAVVGLSGWLPCSRSLRNQVSVSH 180
           KLGIGGFSMGAATAIYSAS RILGQYGNGNLYPINLSAVVGLSGWLPCSRSLRNQVS+SH
Sbjct: 121 KLGIGGFSMGAATAIYSASCRILGQYGNGNLYPINLSAVVGLSGWLPCSRSLRNQVSMSH 180

Query: 181 EAARRAASLPILLCHGSGDDVVAYKHGEKSAHTLSSSGFSNLTFKSYNGLGHYTIPEEMD 240
           EAARRAASLPILLCHGSGDDVVAYKHGEKSAHTLSSSGF NLTFKSYNGLGHYTIPEEMD
Sbjct: 181 EAARRAASLPILLCHGSGDDVVAYKHGEKSAHTLSSSGFRNLTFKSYNGLGHYTIPEEMD 240

Query: 241 VVCNWLTVVLGLDGLRLVDH 261
           VVCNWLTVVL LDG+RLVDH
Sbjct: 241 VVCNWLTVVLALDGVRLVDH 260

BLAST of Carg09324-RA vs. NCBI nr
Match: XP_004138733.1 (PREDICTED: acyl-protein thioesterase 2 [Cucumis sativus] >XP_011649812.1 PREDICTED: acyl-protein thioesterase 2 [Cucumis sativus] >XP_011649813.1 PREDICTED: acyl-protein thioesterase 2 [Cucumis sativus] >XP_011649814.1 PREDICTED: acyl-protein thioesterase 2 [Cucumis sativus] >KGN62923.1 hypothetical protein Csa_2G380030 [Cucumis sativus])

HSP 1 Score: 515.4 bits (1326), Expect = 1.1e-142
Identity = 245/260 (94.23%), Postives = 255/260 (98.08%), Query Frame = 0

Query: 1   MSYNSSAMGSGSRTGRMSFEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLP 60
           MSYNSS +GSGSRTGRM+FEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQ+LETLPLP
Sbjct: 1   MSYNSSTVGSGSRTGRMTFEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQILETLPLP 60

Query: 61  NIKWICPTAPTRPVTLFGGFPCTAWFDVGDISEESPDDLEGLDAAASHVANLLSTEPADI 120
           NIKWICPTAPTRPV LFGGFPCTAWFDVGDISE+SPDDLEGLDAAASHVANLLSTEPADI
Sbjct: 61  NIKWICPTAPTRPVALFGGFPCTAWFDVGDISEDSPDDLEGLDAAASHVANLLSTEPADI 120

Query: 121 KLGIGGFSMGAATAIYSASRRILGQYGNGNLYPINLSAVVGLSGWLPCSRSLRNQVSVSH 180
           KLGIGGFSMGAATAIYSAS RILGQYGNGNLYPINLSAVVGLSGWLPCSRSLRNQ++VSH
Sbjct: 121 KLGIGGFSMGAATAIYSASCRILGQYGNGNLYPINLSAVVGLSGWLPCSRSLRNQINVSH 180

Query: 181 EAARRAASLPILLCHGSGDDVVAYKHGEKSAHTLSSSGFSNLTFKSYNGLGHYTIPEEMD 240
           EAARRAA LPILLCHGSGDDVVAYKHGEKSAHTLSS+GF NLTFK+YNGLGHYTIPEEM+
Sbjct: 181 EAARRAACLPILLCHGSGDDVVAYKHGEKSAHTLSSAGFRNLTFKTYNGLGHYTIPEEMN 240

Query: 241 VVCNWLTVVLGLDGLRLVDH 261
           VVCNWLTV+LGLDGLRLVDH
Sbjct: 241 VVCNWLTVILGLDGLRLVDH 260

BLAST of Carg09324-RA vs. NCBI nr
Match: XP_008445177.1 (PREDICTED: acyl-protein thioesterase 2-like [Cucumis melo] >XP_008445178.1 PREDICTED: acyl-protein thioesterase 2-like [Cucumis melo] >XP_008445179.1 PREDICTED: acyl-protein thioesterase 2-like [Cucumis melo] >XP_008445180.1 PREDICTED: acyl-protein thioesterase 2-like [Cucumis melo] >XP_016899931.1 PREDICTED: acyl-protein thioesterase 2-like [Cucumis melo])

HSP 1 Score: 513.5 bits (1321), Expect = 4.3e-142
Identity = 245/260 (94.23%), Postives = 252/260 (96.92%), Query Frame = 0

Query: 1   MSYNSSAMGSGSRTGRMSFEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLP 60
           MSYNSS +GSGSRTGRM+FEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLP
Sbjct: 1   MSYNSSTVGSGSRTGRMTFEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLP 60

Query: 61  NIKWICPTAPTRPVTLFGGFPCTAWFDVGDISEESPDDLEGLDAAASHVANLLSTEPADI 120
           NIKWICPTAPTRPV LFGGFPCTAWFDVGDISE+SPDDLEGLDAAASHVANLLSTEP DI
Sbjct: 61  NIKWICPTAPTRPVALFGGFPCTAWFDVGDISEDSPDDLEGLDAAASHVANLLSTEPGDI 120

Query: 121 KLGIGGFSMGAATAIYSASRRILGQYGNGNLYPINLSAVVGLSGWLPCSRSLRNQVSVSH 180
           KLGIGGFSMGAATAIYSAS RILGQYGNGNLYPINLSAVVGLSGWLPCSRSLRNQ+ VSH
Sbjct: 121 KLGIGGFSMGAATAIYSASCRILGQYGNGNLYPINLSAVVGLSGWLPCSRSLRNQIGVSH 180

Query: 181 EAARRAASLPILLCHGSGDDVVAYKHGEKSAHTLSSSGFSNLTFKSYNGLGHYTIPEEMD 240
           EAA RAASLPILLCHGSGDDVVAYKHGEKSAHTLSS+GF NLTFK+YNGLGHYTIPEEMD
Sbjct: 181 EAAMRAASLPILLCHGSGDDVVAYKHGEKSAHTLSSAGFRNLTFKAYNGLGHYTIPEEMD 240

Query: 241 VVCNWLTVVLGLDGLRLVDH 261
           VVC WLTV+LGLDGLRLVDH
Sbjct: 241 VVCQWLTVILGLDGLRLVDH 260

BLAST of Carg09324-RA vs. TAIR10
Match: AT5G20060.2 (alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 373.6 bits (958), Expect = 9.7e-104
Identity = 174/254 (68.50%), Postives = 212/254 (83.46%), Query Frame = 0

Query: 1   MSYNSSAMGSGSRTGRMSFEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLP 60
           MS + +A+GSG R  R + EFG+THVVRPKGKHQATIVWLHGLGD GSSWSQLLETLPLP
Sbjct: 1   MSISGAAVGSG-RNLRRAVEFGKTHVVRPKGKHQATIVWLHGLGDNGSSWSQLLETLPLP 60

Query: 61  NIKWICPTAPTRPVTLFGGFPCTAWFDVGDISEESPDDLEGLDAAASHVANLLSTEPADI 120
           NIKWICPTAP++P++LFGGFP TAWFDV DI+E+ PDD+EGLD AA+HVANLLS EPADI
Sbjct: 61  NIKWICPTAPSQPISLFGGFPSTAWFDVVDINEDGPDDMEGLDVAAAHVANLLSNEPADI 120

Query: 121 KLGIGGFSMGAATAIYSASRRILGQYGNGNLYPINLSAVVGLSGWLPCSRSLRNQVSVSH 180
           KLG+GGFSMGAAT++YSA+   LG+YGNGN YPINLSA++GLSGWLPC+++L  ++    
Sbjct: 121 KLGVGGFSMGAATSLYSATCFALGKYGNGNPYPINLSAIIGLSGWLPCAKTLAGKLE-EE 180

Query: 181 EAARRAASLPILLCHGSGDDVVAYKHGEKSAHTLSSSGFSNLTFKSYNGLGHYTIPEEMD 240
           +   RAASLPI++CHG  DDVV +K GEKS+  L S+GF  +TFK Y+ LGH+TIP+E+D
Sbjct: 181 QIKNRAASLPIVVCHGKADDVVPFKFGEKSSQALLSNGFKKVTFKPYSALGHHTIPQELD 240

Query: 241 VVCNWLTVVLGLDG 255
            +C WLT  L L+G
Sbjct: 241 ELCAWLTSTLSLEG 252

BLAST of Carg09324-RA vs. TAIR10
Match: AT1G52700.1 (alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 372.9 bits (956), Expect = 1.7e-103
Identity = 178/256 (69.53%), Postives = 212/256 (82.81%), Query Frame = 0

Query: 1   MSYNSSAMGSGSRTGRMSFEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLP 60
           MSY+  +MGSGSR  R  +EFGRT+VVRPKGKHQAT+VWLHGLGD GSS SQL+++L LP
Sbjct: 1   MSYSHQSMGSGSRNAR-GYEFGRTYVVRPKGKHQATLVWLHGLGDNGSSSSQLMDSLHLP 60

Query: 61  NIKWICPTAPTRPVTLFGGFPCTAWFDVGDISEESPDDLEGLDAAASHVANLLSTEPADI 120
           NIKWICPTAP+RPVT  GGF CTAWFDVG+ISE+  DDLEGLDA+ASH+ANLLS+EPAD+
Sbjct: 61  NIKWICPTAPSRPVTSLGGFTCTAWFDVGEISEDGHDDLEGLDASASHIANLLSSEPADV 120

Query: 121 KLGIGGFSMGAATAIYSASRRILGQYGNGNLYPINLSAVVGLSGWLPCSRSLRNQVSVSH 180
           K+GIGGFSMGAA ++YSA+   LG+YG G+ YPINL AVVGLSGWLP  +SLR+++  S 
Sbjct: 121 KVGIGGFSMGAAISLYSATCYALGRYGTGHAYPINLQAVVGLSGWLPGWKSLRSKIECSF 180

Query: 181 EAARRAASLPILLCHGSGDDVVAYKHGEKSAHTLSSSGFSNLTFKSYNGLGHYTIPEEMD 240
           EAARRAASLPI+L HG+ DDVV Y+ GEKSA +L  +GF    FK Y GLGHYT+P EMD
Sbjct: 181 EAARRAASLPIILTHGTSDDVVPYRFGEKSAQSLGMAGFRLAMFKPYEGLGHYTVPREMD 240

Query: 241 VVCNWLTVVLGLDGLR 257
            V +WLT +LGL+G R
Sbjct: 241 EVVHWLTTMLGLEGSR 255

BLAST of Carg09324-RA vs. TAIR10
Match: AT3G15650.2 (alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 362.1 bits (928), Expect = 2.9e-100
Identity = 178/275 (64.73%), Postives = 212/275 (77.09%), Query Frame = 0

Query: 1   MSYNSSAMGSGSRTGRMSFEFGRTHVVRPKGKHQATIVWLHGLGDKGS------------ 60
           MSY+  +MGSGSR+ R  +EFGRT+VVRPKGKHQATIVWLHGLGD GS            
Sbjct: 1   MSYSRQSMGSGSRSTR-GYEFGRTYVVRPKGKHQATIVWLHGLGDNGSRILACSLITTSH 60

Query: 61  -------SWSQLLETLPLPNIKWICPTAPTRPVTLFGGFPCTAWFDVGDISEESPDDLEG 120
                  S SQLLE+LPLPNIKWICPTAP+RPV+L GGFPCTAWFDVG+ISE+  DD+EG
Sbjct: 61  FGSVSFCSSSQLLESLPLPNIKWICPTAPSRPVSLLGGFPCTAWFDVGEISEDLHDDIEG 120

Query: 121 LDAAASHVANLLSTEPADIKLGIGGFSMGAATAIYSASRRILGQYGNGNLYPINLSAVVG 180
           LDA+A+H+ANLLS EP D+K+GIGGFSMGAA A+YS +   LG+YG G+ Y INL A VG
Sbjct: 121 LDASAAHIANLLSAEPTDVKVGIGGFSMGAAIALYSTTCYALGRYGTGHAYTINLRATVG 180

Query: 181 LSGWLPCSRSLRNQVSVSHEAARRAASLPILLCHGSGDDVVAYKHGEKSAHTLSSSGFSN 240
           LSGWLP  RSLR+++  S+E ARRAAS+PILL HG+ DDVV Y+ GEKSAH+L+ +GF  
Sbjct: 181 LSGWLPGWRSLRSKIESSNEVARRAASIPILLAHGTSDDVVPYRFGEKSAHSLAMAGFRQ 240

Query: 241 LTFKSYNGLGHYTIPEEMDVVCNWLTVVLGLDGLR 257
             FK Y GLGHYT+P+EMD V +WL   LGL+G R
Sbjct: 241 TMFKPYEGLGHYTVPKEMDEVVHWLVSRLGLEGSR 274

BLAST of Carg09324-RA vs. TAIR10
Match: AT1G52695.1 (alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 160.6 bits (405), Expect = 1.3e-39
Identity = 93/223 (41.70%), Postives = 123/223 (55.16%), Query Frame = 0

Query: 8   MGSGSRTGRMSFEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLPNIKWICP 67
           M SGS       EFG+ + V P G H+ATIVWLH +G+ G +  + L+ L LPNIKWICP
Sbjct: 1   MASGS-INVSGLEFGQINTVYPTGIHKATIVWLHDVGNTGFNSLEPLQNLRLPNIKWICP 60

Query: 68  TAPTRPVTLFGGFPCTAWFDVGDISEESPDDLEGLDAAASHVANLLSTEPADIKLGIGGF 127
           TAP R VT  GG    AW D+  +SE   DD   L+    ++ +L S EP ++  G+ G 
Sbjct: 61  TAPRRRVTSLGGEITNAWCDIAKVSENMQDDFGTLNYVNEYITSLFSNEPQNVIKGVAGL 120

Query: 128 SMGAATAIYSASRRILGQYGNGNLYPINLSAVVGLSGWLPCSRSLR-NQVSVSHEAARRA 187
            +GAA A+Y  S    G        PIN   V+G++GWLP  R L  N  + +   A RA
Sbjct: 121 GLGAAQALYYTSCYAFG------WVPINPQIVIGINGWLPGWRRLEYNMNNTNFGTANRA 180

Query: 188 ASLPILLCHGSGDDVVAYKHGEKSAHTLSSSGFSNLTFKSYNG 230
           A+  IL+ HG+ DDVV    G + A +L  +GF  L FK   G
Sbjct: 181 AASKILILHGTSDDVVPSSFGYRCADSLRMAGFPTL-FKQCGG 215

BLAST of Carg09324-RA vs. TAIR10
Match: AT1G52470.1 (alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 129.8 bits (325), Expect = 2.4e-30
Identity = 81/241 (33.61%), Postives = 122/241 (50.62%), Query Frame = 0

Query: 10  SGSRTGRMSFEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLPNIKWICPTA 69
           S SR G    +   +H V P+G  +A+IVWLH   +  +   Q +++L L N+ WICP  
Sbjct: 4   SSSRNGANVLDLKCSHFVEPQGDQRASIVWLHDKDEHFTDSVQFVKSLKLKNVNWICP-- 63

Query: 70  PTRPVTLFGGFPCTAWFDVGDISEESPDDLEGLDAAASHVANLLSTEPADIKLGIGGFSM 129
              P+           +D G  S    DD E LD+AA  VA+LL  EP ++  G+GGF M
Sbjct: 64  ---PIVY-----TNTSYDFG--SNIKQDDREALDSAAKFVADLLLREPLNVVKGVGGFGM 123

Query: 130 GAATAIYSASRRILGQYGNGNLYPINLSAVVGLSGWLPCSRSLRNQVSVSHEAARRAASL 189
           GA  A+  A+   LG       YPIN   VVG++GWL  + S+ + +  +  A  RAAS 
Sbjct: 124 GAVVALQFATNCALGH------YPINPRVVVGINGWLSITGSITSSIEYTVGAVARAASQ 183

Query: 190 PILLCHGSGDDVVAYKHGEKSAHTLSSSGFSNLTFKSYNGLGHYTIPEEMDVVCNWLTVV 249
            I    G+ + ++ Y   E+   +L  +GF ++ F  Y+ L H    +  D++  WL + 
Sbjct: 184 KIFFTRGAENRLLPYTREEEVVESLREAGFGDVFFLIYSWLRHEHHLDIRDMLKLWLELS 226

Query: 250 L 251
           L
Sbjct: 244 L 226

BLAST of Carg09324-RA vs. Swiss-Prot
Match: sp|O95372|LYPA2_HUMAN (Acyl-protein thioesterase 2 OS=Homo sapiens OX=9606 GN=LYPLA2 PE=1 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 1.7e-28
Identity = 79/225 (35.11%), Postives = 120/225 (53.33%), Query Frame = 0

Query: 32  KHQATIVWLHGLGDKGSSWSQLLETLPLPNIKWICPTAPTRPVTLFGGFPCTAWFDVGDI 91
           +  A +++LHGLGD G SW+  L T+ LP++K+ICP AP  PVTL       +WFD+  +
Sbjct: 22  RETAAVIFLHGLGDTGHSWADALSTIRLPHVKYICPHAPRIPVTLNMKMVMPSWFDLMGL 81

Query: 92  SEESPDDLEGLDAAASHVANLLSTE-----PADIKLGIGGFSMGAATAIYSASRRILGQY 151
           S ++P+D  G+  AA ++  L+  E     PA+ ++ +GGFS G A ++Y+A        
Sbjct: 82  SPDAPEDEAGIKKAAENIKALIEHEMKNGIPAN-RIVLGGFSQGGALSLYTAL------- 141

Query: 152 GNGNLYPINLSAVVGLSGWLPCSRSLRNQVSVSHEAARRAASLPILLCHGSGDDVVAYKH 211
                 P  L+ +V LS WLP  R+     + S      A  L IL CHG  D +V  + 
Sbjct: 142 ----TCPHPLAGIVALSCWLPLHRAFPQAANGS------AKDLAILQCHGELDPMVPVRF 201

Query: 212 GEKSAHTLSSSGF-SNLTFKSYNGLGHYTIPEEMDVVCNWLTVVL 251
           G  +A  L S    + + FK+Y G+ H + P+EM  V  +L  +L
Sbjct: 202 GALTAEKLRSVVTPARVQFKTYPGVMHSSCPQEMAAVKEFLEKLL 228

BLAST of Carg09324-RA vs. Swiss-Prot
Match: sp|Q9WTL7|LYPA2_MOUSE (Acyl-protein thioesterase 2 OS=Mus musculus OX=10090 GN=Lypla2 PE=1 SV=1)

HSP 1 Score: 126.7 bits (317), Expect = 3.7e-28
Identity = 78/225 (34.67%), Postives = 120/225 (53.33%), Query Frame = 0

Query: 32  KHQATIVWLHGLGDKGSSWSQLLETLPLPNIKWICPTAPTRPVTLFGGFPCTAWFDVGDI 91
           +  A +++LHGLGD G SW+  L T+ LP++K+ICP AP  PVTL       +WFD+  +
Sbjct: 22  RETAAVIFLHGLGDTGHSWADALSTIRLPHVKYICPHAPRIPVTLNMKMVMPSWFDLMGL 81

Query: 92  SEESPDDLEGLDAAASHVANLLSTE-----PADIKLGIGGFSMGAATAIYSASRRILGQY 151
           S ++P+D  G+  AA ++  L+  E     PA+ ++ +GGFS G A ++Y+A        
Sbjct: 82  SPDAPEDEAGIKKAAENIKALIEHEMKNGIPAN-RIVLGGFSQGGALSLYTAL------- 141

Query: 152 GNGNLYPINLSAVVGLSGWLPCSRSLRNQVSVSHEAARRAASLPILLCHGSGDDVVAYKH 211
                 P  L+ +V LS WLP  R+     + S      A  L IL CHG  D +V  + 
Sbjct: 142 ----TCPHPLAGIVALSCWLPLHRNFPQAANGS------AKDLAILQCHGELDPMVPVRF 201

Query: 212 GEKSAHTLSSSGF-SNLTFKSYNGLGHYTIPEEMDVVCNWLTVVL 251
           G  +A  L +    + + FK+Y G+ H + P+EM  V  +L  +L
Sbjct: 202 GALTAEKLRTVVTPARVQFKTYPGVMHSSCPQEMAAVKEFLEKLL 228

BLAST of Carg09324-RA vs. Swiss-Prot
Match: sp|Q9QYL8|LYPA2_RAT (Acyl-protein thioesterase 2 OS=Rattus norvegicus OX=10116 GN=Lypla2 PE=1 SV=1)

HSP 1 Score: 126.7 bits (317), Expect = 3.7e-28
Identity = 78/225 (34.67%), Postives = 120/225 (53.33%), Query Frame = 0

Query: 32  KHQATIVWLHGLGDKGSSWSQLLETLPLPNIKWICPTAPTRPVTLFGGFPCTAWFDVGDI 91
           +  A +++LHGLGD G SW+  L T+ LP++K+ICP AP  PVTL       +WFD+  +
Sbjct: 22  RETAAVIFLHGLGDTGHSWADALSTIRLPHVKYICPHAPRIPVTLNMKMVMPSWFDLMGL 81

Query: 92  SEESPDDLEGLDAAASHVANLLSTE-----PADIKLGIGGFSMGAATAIYSASRRILGQY 151
           S ++P+D  G+  AA ++  L+  E     PA+ ++ +GGFS G A ++Y+A        
Sbjct: 82  SPDAPEDEAGIKKAAENIKALIEHEMKNGIPAN-RIVLGGFSQGGALSLYTAL------- 141

Query: 152 GNGNLYPINLSAVVGLSGWLPCSRSLRNQVSVSHEAARRAASLPILLCHGSGDDVVAYKH 211
                 P  L+ +V LS WLP  R+     + S      A  L IL CHG  D +V  + 
Sbjct: 142 ----TCPHPLAGIVALSCWLPLHRNFPQAANGS------AKDLAILQCHGELDPMVPVRF 201

Query: 212 GEKSAHTLSSSGF-SNLTFKSYNGLGHYTIPEEMDVVCNWLTVVL 251
           G  +A  L +    + + FK+Y G+ H + P+EM  V  +L  +L
Sbjct: 202 GALTAEKLRTVVTPARVQFKTYPGVMHSSCPQEMAAVKEFLEKLL 228

BLAST of Carg09324-RA vs. Swiss-Prot
Match: sp|Q54T49|APT11_DICDI (Acyl-protein thioesterase 1 homolog 1 OS=Dictyostelium discoideum OX=44689 GN=DDB_G0282005 PE=2 SV=1)

HSP 1 Score: 119.4 bits (298), Expect = 5.9e-26
Identity = 70/227 (30.84%), Postives = 119/227 (52.42%), Query Frame = 0

Query: 33  HQATIVWLHGLGDKGSSWSQLLETLPLPN---IKWICPTAPTRPVTLFGGFPCTAWFDVG 92
           H AT+++ HGLGD G+ W +++E +   N   I++ICP AP + VTL GGF   +W+D+ 
Sbjct: 18  HSATVIFSHGLGDSGAGWIEVMEEIQSRNNGHIRFICPNAPIQAVTLNGGFKMPSWYDIK 77

Query: 93  DISEESPDDLEGLDAAASHVANLLSTEPADIKLG-----IGGFSMGAATAIYSASRRILG 152
            +S    +D   +D + + +  ++  E  + K+      IGGFS GAA ++Y+   +   
Sbjct: 78  SLSSRGDEDPAQVDESKNIIETIIKHEMEEEKIPAERIIIGGFSQGAALSLYTFYSQT-- 137

Query: 153 QYGNGNLYPINLSAVVGLSGWLP-CSRSLRNQVSVSHEAARRAASLPILLCHGSGDDVVA 212
                      L   + LSG+LP  ++ + N ++            P+L+ HG  D VV 
Sbjct: 138 --------ETKLGGCIALSGYLPLATKFVANSLNKEQ---------PLLMIHGDCDQVVR 197

Query: 213 YKHGEKSAHTLSSSGFSNLTFKSYNGLGHYTIPEEMDVVCNWLTVVL 251
           ++ G+ S   L S G  N  F +  GLGH++ PEE+D++  +++  L
Sbjct: 198 HQWGKLSFDHLKSQGI-NGEFITLKGLGHHSSPEEIDLMTKFISKTL 224

BLAST of Carg09324-RA vs. Swiss-Prot
Match: sp|P0CL95|APTH1_CRYNB (Acyl-protein thioesterase 1 OS=Cryptococcus neoformans var. neoformans serotype D (strain B-3501A) OX=283643 GN=CNBF2260 PE=3 SV=1)

HSP 1 Score: 116.3 bits (290), Expect = 5.0e-25
Identity = 74/247 (29.96%), Postives = 119/247 (48.18%), Query Frame = 0

Query: 27  VRPKGKHQATIVWLHGLGDKGSSWSQLLETL--PLPNIKWICPTAPTRPVTLFGGFPCTA 86
           + PK  H AT+++LHGLGD G  W  + + L    PN+KWI P APT PV+L  G    +
Sbjct: 10  ISPKEAHTATVIFLHGLGDSGHGWLPVAKMLWSSFPNVKWILPHAPTIPVSLNHGMAMPS 69

Query: 87  WFDV---GDISEESPDDLEGLDAAASHVANLLSTEP----ADIKLGIGGFSMGAATAIYS 146
           WFD+     +     DD +G+      V  L+  E      + ++ +GGFS G A ++ +
Sbjct: 70  WFDIRHLDKLDNSENDDEQGMLETLKSVDELIQAEVDSGIPENRIVLGGFSQGGAISVLN 129

Query: 147 --ASRRILGQYGNGNLYPINLSAVVGLSGWLPCSRSLRNQVSVSHEAARRAASLPILLCH 206
              ++R              L+ VV LS W+P +  +   +S        A  +P+   H
Sbjct: 130 MLTTKR-------------KLAGVVALSTWVPLNHKIVQMMS------EHAKDIPVFWGH 189

Query: 207 GSGDDVVAYKHGEKS--------AHTLSSSGFS----NLTFKSYNGLGHYTIPEEMDVVC 251
           G+ D VV Y+ G++S         + L S G +     + F+SY G+ H + P+E++ + 
Sbjct: 190 GTNDPVVDYRFGQRSVDFLVQKCGYKLLSQGTTFARPGIRFESYPGMPHSSCPQEIEDLK 237

BLAST of Carg09324-RA vs. TrEMBL
Match: tr|A0A0A0LM90|A0A0A0LM90_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G380030 PE=4 SV=1)

HSP 1 Score: 515.4 bits (1326), Expect = 7.5e-143
Identity = 245/260 (94.23%), Postives = 255/260 (98.08%), Query Frame = 0

Query: 1   MSYNSSAMGSGSRTGRMSFEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLP 60
           MSYNSS +GSGSRTGRM+FEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQ+LETLPLP
Sbjct: 1   MSYNSSTVGSGSRTGRMTFEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQILETLPLP 60

Query: 61  NIKWICPTAPTRPVTLFGGFPCTAWFDVGDISEESPDDLEGLDAAASHVANLLSTEPADI 120
           NIKWICPTAPTRPV LFGGFPCTAWFDVGDISE+SPDDLEGLDAAASHVANLLSTEPADI
Sbjct: 61  NIKWICPTAPTRPVALFGGFPCTAWFDVGDISEDSPDDLEGLDAAASHVANLLSTEPADI 120

Query: 121 KLGIGGFSMGAATAIYSASRRILGQYGNGNLYPINLSAVVGLSGWLPCSRSLRNQVSVSH 180
           KLGIGGFSMGAATAIYSAS RILGQYGNGNLYPINLSAVVGLSGWLPCSRSLRNQ++VSH
Sbjct: 121 KLGIGGFSMGAATAIYSASCRILGQYGNGNLYPINLSAVVGLSGWLPCSRSLRNQINVSH 180

Query: 181 EAARRAASLPILLCHGSGDDVVAYKHGEKSAHTLSSSGFSNLTFKSYNGLGHYTIPEEMD 240
           EAARRAA LPILLCHGSGDDVVAYKHGEKSAHTLSS+GF NLTFK+YNGLGHYTIPEEM+
Sbjct: 181 EAARRAACLPILLCHGSGDDVVAYKHGEKSAHTLSSAGFRNLTFKTYNGLGHYTIPEEMN 240

Query: 241 VVCNWLTVVLGLDGLRLVDH 261
           VVCNWLTV+LGLDGLRLVDH
Sbjct: 241 VVCNWLTVILGLDGLRLVDH 260

BLAST of Carg09324-RA vs. TrEMBL
Match: tr|A0A1S4DVB8|A0A1S4DVB8_CUCME (acyl-protein thioesterase 2-like OS=Cucumis melo OX=3656 GN=LOC103488285 PE=4 SV=1)

HSP 1 Score: 513.5 bits (1321), Expect = 2.9e-142
Identity = 245/260 (94.23%), Postives = 252/260 (96.92%), Query Frame = 0

Query: 1   MSYNSSAMGSGSRTGRMSFEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLP 60
           MSYNSS +GSGSRTGRM+FEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLP
Sbjct: 1   MSYNSSTVGSGSRTGRMTFEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLP 60

Query: 61  NIKWICPTAPTRPVTLFGGFPCTAWFDVGDISEESPDDLEGLDAAASHVANLLSTEPADI 120
           NIKWICPTAPTRPV LFGGFPCTAWFDVGDISE+SPDDLEGLDAAASHVANLLSTEP DI
Sbjct: 61  NIKWICPTAPTRPVALFGGFPCTAWFDVGDISEDSPDDLEGLDAAASHVANLLSTEPGDI 120

Query: 121 KLGIGGFSMGAATAIYSASRRILGQYGNGNLYPINLSAVVGLSGWLPCSRSLRNQVSVSH 180
           KLGIGGFSMGAATAIYSAS RILGQYGNGNLYPINLSAVVGLSGWLPCSRSLRNQ+ VSH
Sbjct: 121 KLGIGGFSMGAATAIYSASCRILGQYGNGNLYPINLSAVVGLSGWLPCSRSLRNQIGVSH 180

Query: 181 EAARRAASLPILLCHGSGDDVVAYKHGEKSAHTLSSSGFSNLTFKSYNGLGHYTIPEEMD 240
           EAA RAASLPILLCHGSGDDVVAYKHGEKSAHTLSS+GF NLTFK+YNGLGHYTIPEEMD
Sbjct: 181 EAAMRAASLPILLCHGSGDDVVAYKHGEKSAHTLSSAGFRNLTFKAYNGLGHYTIPEEMD 240

Query: 241 VVCNWLTVVLGLDGLRLVDH 261
           VVC WLTV+LGLDGLRLVDH
Sbjct: 241 VVCQWLTVILGLDGLRLVDH 260

BLAST of Carg09324-RA vs. TrEMBL
Match: tr|B9IKU8|B9IKU8_POPTR (Uncharacterized protein OS=Populus trichocarpa OX=3694 GN=POPTR_018G030200v3 PE=4 SV=1)

HSP 1 Score: 458.4 bits (1178), Expect = 1.1e-125
Identity = 215/254 (84.65%), Postives = 237/254 (93.31%), Query Frame = 0

Query: 1   MSYNSSAMGSGSRTGRMSFEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLP 60
           MSYN+SA+GSGSR    +FEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLP
Sbjct: 1   MSYNTSAVGSGSRNTTRTFEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLP 60

Query: 61  NIKWICPTAPTRPVTLFGGFPCTAWFDVGDISEESPDDLEGLDAAASHVANLLSTEPADI 120
           NIKWICPTAPTRPV +FGGFPCTAWFDVGDISE++PDDLEGL+A+A+HVANLLSTEPADI
Sbjct: 61  NIKWICPTAPTRPVAIFGGFPCTAWFDVGDISEDAPDDLEGLEASATHVANLLSTEPADI 120

Query: 121 KLGIGGFSMGAATAIYSASRRILGQYGNGNLYPINLSAVVGLSGWLPCSRSLRNQVSVSH 180
           KLG+GGFSMGAATA+YSA+  +LGQYGNGN YPINL+AVVGLSGWLPCSR+LR+++  S 
Sbjct: 121 KLGVGGFSMGAATALYSATCHVLGQYGNGNQYPINLTAVVGLSGWLPCSRTLRSRIERSD 180

Query: 181 EAARRAASLPILLCHGSGDDVVAYKHGEKSAHTLSSSGFSNLTFKSYNGLGHYTIPEEMD 240
           EAARRAASLPILLCHGSGDDVVA+KHGEKSA  LSS+GF NLTF+SYNGLGHYTIPEEMD
Sbjct: 181 EAARRAASLPILLCHGSGDDVVAHKHGEKSAQALSSAGFRNLTFRSYNGLGHYTIPEEMD 240

Query: 241 VVCNWLTVVLGLDG 255
            VC+WLT  LGLDG
Sbjct: 241 EVCHWLTTRLGLDG 254

BLAST of Carg09324-RA vs. TrEMBL
Match: tr|A0A2K2A818|A0A2K2A818_POPTR (Uncharacterized protein OS=Populus trichocarpa OX=3694 GN=POPTR_006G251100v3 PE=4 SV=1)

HSP 1 Score: 458.0 bits (1177), Expect = 1.4e-125
Identity = 215/256 (83.98%), Postives = 235/256 (91.80%), Query Frame = 0

Query: 1   MSYNSSAMGSGSRTGRMSFEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLP 60
           MSYN+SA GSGSR    +FEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLP
Sbjct: 1   MSYNTSAAGSGSRNTTRTFEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLP 60

Query: 61  NIKWICPTAPTRPVTLFGGFPCTAWFDVGDISEESPDDLEGLDAAASHVANLLSTEPADI 120
           NIKWICPTAPTRPV +FGGFPCTAW D GDISE++PDDLEGLDA+A+HVANLLSTEPADI
Sbjct: 61  NIKWICPTAPTRPVAIFGGFPCTAWSDAGDISEDAPDDLEGLDASAAHVANLLSTEPADI 120

Query: 121 KLGIGGFSMGAATAIYSASRRILGQYGNGNLYPINLSAVVGLSGWLPCSRSLRNQVSVSH 180
           KLG+GGFSMGAATA+YSA+  I GQYGNGNLYP+NLSA+VGLSGWLPCSR+LRN++  S 
Sbjct: 121 KLGVGGFSMGAATALYSATCHIFGQYGNGNLYPVNLSAIVGLSGWLPCSRTLRNRMERSD 180

Query: 181 EAARRAASLPILLCHGSGDDVVAYKHGEKSAHTLSSSGFSNLTFKSYNGLGHYTIPEEMD 240
           EAARRAASLPILLCHGSGDDVVA+KHGEKSA  LSS+GF NLTF+SYNGLGHYTIPEEMD
Sbjct: 181 EAARRAASLPILLCHGSGDDVVAHKHGEKSAQALSSAGFRNLTFRSYNGLGHYTIPEEMD 240

Query: 241 VVCNWLTVVLGLDGLR 257
            VCNWLT  +GL+G R
Sbjct: 241 EVCNWLTTRIGLEGPR 256

BLAST of Carg09324-RA vs. TrEMBL
Match: tr|A0A2P5DUI3|A0A2P5DUI3_9ROSA (Phospholipase/carboxylesterase/thioesterase OS=Trema orientalis OX=63057 GN=TorRG33x02_241300 PE=4 SV=1)

HSP 1 Score: 454.1 bits (1167), Expect = 2.1e-124
Identity = 213/256 (83.20%), Postives = 237/256 (92.58%), Query Frame = 0

Query: 1   MSYNSSAMGSGSRTGRMSFEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLP 60
           MSYN+S++GSGSRT R +FEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPL 
Sbjct: 1   MSYNNSSIGSGSRTARRTFEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLS 60

Query: 61  NIKWICPTAPTRPVTLFGGFPCTAWFDVGDISEESPDDLEGLDAAASHVANLLSTEPADI 120
           NIKWICPTAPTRPV LFGGFPCTAWFD+GDISE++PDDLEGLDA+A+HVANLLSTEPADI
Sbjct: 61  NIKWICPTAPTRPVALFGGFPCTAWFDMGDISEDAPDDLEGLDASAAHVANLLSTEPADI 120

Query: 121 KLGIGGFSMGAATAIYSASRRILGQYGNGNLYPINLSAVVGLSGWLPCSRSLRNQVSVSH 180
           KLGIGGFSMGAATA+YSA+ R+LG+YGNGNLYP+NLSAVVGLSGWLPCSR+LR+ +  S 
Sbjct: 121 KLGIGGFSMGAATALYSATCRVLGKYGNGNLYPVNLSAVVGLSGWLPCSRTLRSLMEGSV 180

Query: 181 EAARRAASLPILLCHGSGDDVVAYKHGEKSAHTLSSSGFSNLTFKSYNGLGHYTIPEEMD 240
           EAARRAAS+PIL+CHG  DDVVAY HG+KSA TLSS+GF NLTF++YNGLGHYT+PEE D
Sbjct: 181 EAARRAASVPILMCHGLVDDVVAYHHGDKSAQTLSSTGFQNLTFRTYNGLGHYTVPEETD 240

Query: 241 VVCNWLTVVLGLDGLR 257
            VCNWLT  LGLDG R
Sbjct: 241 EVCNWLTANLGLDGSR 256

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022961761.14.0e-14899.62acyl-protein thioesterase 2-like [Cucurbita moschata][more]
XP_022996560.11.3e-14698.08acyl-protein thioesterase 2-like [Cucurbita maxima] >XP_022996561.1 acyl-protein... [more]
XP_023545446.11.1e-14597.69acyl-protein thioesterase 2-like [Cucurbita pepo subsp. pepo] >XP_023545447.1 ac... [more]
XP_004138733.11.1e-14294.23PREDICTED: acyl-protein thioesterase 2 [Cucumis sativus] >XP_011649812.1 PREDICT... [more]
XP_008445177.14.3e-14294.23PREDICTED: acyl-protein thioesterase 2-like [Cucumis melo] >XP_008445178.1 PREDI... [more]
Match NameE-valueIdentityDescription
AT5G20060.29.7e-10468.50alpha/beta-Hydrolases superfamily protein[more]
AT1G52700.11.7e-10369.53alpha/beta-Hydrolases superfamily protein[more]
AT3G15650.22.9e-10064.73alpha/beta-Hydrolases superfamily protein[more]
AT1G52695.11.3e-3941.70alpha/beta-Hydrolases superfamily protein[more]
AT1G52470.12.4e-3033.61alpha/beta-Hydrolases superfamily protein[more]
Match NameE-valueIdentityDescription
sp|O95372|LYPA2_HUMAN1.7e-2835.11Acyl-protein thioesterase 2 OS=Homo sapiens OX=9606 GN=LYPLA2 PE=1 SV=1[more]
sp|Q9WTL7|LYPA2_MOUSE3.7e-2834.67Acyl-protein thioesterase 2 OS=Mus musculus OX=10090 GN=Lypla2 PE=1 SV=1[more]
sp|Q9QYL8|LYPA2_RAT3.7e-2834.67Acyl-protein thioesterase 2 OS=Rattus norvegicus OX=10116 GN=Lypla2 PE=1 SV=1[more]
sp|Q54T49|APT11_DICDI5.9e-2630.84Acyl-protein thioesterase 1 homolog 1 OS=Dictyostelium discoideum OX=44689 GN=DD... [more]
sp|P0CL95|APTH1_CRYNB5.0e-2529.96Acyl-protein thioesterase 1 OS=Cryptococcus neoformans var. neoformans serotype ... [more]
Match NameE-valueIdentityDescription
tr|A0A0A0LM90|A0A0A0LM90_CUCSA7.5e-14394.23Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G380030 PE=4 SV=1[more]
tr|A0A1S4DVB8|A0A1S4DVB8_CUCME2.9e-14294.23acyl-protein thioesterase 2-like OS=Cucumis melo OX=3656 GN=LOC103488285 PE=4 SV... [more]
tr|B9IKU8|B9IKU8_POPTR1.1e-12584.65Uncharacterized protein OS=Populus trichocarpa OX=3694 GN=POPTR_018G030200v3 PE=... [more]
tr|A0A2K2A818|A0A2K2A818_POPTR1.4e-12583.98Uncharacterized protein OS=Populus trichocarpa OX=3694 GN=POPTR_006G251100v3 PE=... [more]
tr|A0A2P5DUI3|A0A2P5DUI3_9ROSA2.1e-12483.20Phospholipase/carboxylesterase/thioesterase OS=Trema orientalis OX=63057 GN=TorR... [more]
The following terms have been associated with this mRNA:
Vocabulary: Molecular Function
TermDefinition
GO:0016787hydrolase activity
Vocabulary: INTERPRO
TermDefinition
IPR003140PLipase/COase/thioEstase
IPR029058AB_hydrolase
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0016787 hydrolase activity

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Carg09324Carg09324gene


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Carg09324-RACarg09324-RA-proteinpolypeptide


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg09324-RA.exon.1Carg09324-RA.exon.1exon
Carg09324-RA.exon.2Carg09324-RA.exon.2exon
Carg09324-RA.exon.3Carg09324-RA.exon.3exon
Carg09324-RA.exon.4Carg09324-RA.exon.4exon
Carg09324-RA.exon.5Carg09324-RA.exon.5exon
Carg09324-RA.exon.6Carg09324-RA.exon.6exon
Carg09324-RA.exon.7Carg09324-RA.exon.7exon
Carg09324-RA.exon.8Carg09324-RA.exon.8exon
Carg09324-RA.exon.9Carg09324-RA.exon.9exon
Carg09324-RA.exon.10Carg09324-RA.exon.10exon


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg09324-RA.five_prime_UTR.1Carg09324-RA.five_prime_UTR.1five_prime_UTR
Carg09324-RA.five_prime_UTR.2Carg09324-RA.five_prime_UTR.2five_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg09324-RA.CDS.1Carg09324-RA.CDS.1CDS
Carg09324-RA.CDS.2Carg09324-RA.CDS.2CDS
Carg09324-RA.CDS.3Carg09324-RA.CDS.3CDS
Carg09324-RA.CDS.4Carg09324-RA.CDS.4CDS
Carg09324-RA.CDS.5Carg09324-RA.CDS.5CDS
Carg09324-RA.CDS.6Carg09324-RA.CDS.6CDS
Carg09324-RA.CDS.7Carg09324-RA.CDS.7CDS
Carg09324-RA.CDS.8Carg09324-RA.CDS.8CDS
Carg09324-RA.CDS.9Carg09324-RA.CDS.9CDS


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR029058Alpha/Beta hydrolase foldGENE3DG3DSA:3.40.50.1820coord: 15..251
e-value: 5.6E-62
score: 211.3
IPR029058Alpha/Beta hydrolase foldSUPERFAMILYSSF53474alpha/beta-Hydrolasescoord: 25..247
IPR003140Phospholipase/carboxylesterase/thioesterasePFAMPF02230Abhydrolase_2coord: 25..248
e-value: 6.2E-40
score: 137.2
NoneNo IPR availablePANTHERPTHR10655LYSOPHOSPHOLIPASE-RELATEDcoord: 2..254
NoneNo IPR availablePANTHERPTHR10655:SF34SUBFAMILY NOT NAMEDcoord: 2..254