Carg09228-RA (mRNA) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg09228-RA
Type	mRNA
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	Eukaryotic translation initiation factor 5 like
Location	Cucurbita_argyrosperma_scaffold_018 : 68366 .. 69715 (+)
Sequence length	1350

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGTTACAGAACATTGGTGCCTCAAACCGTGACGATGCCTTCTATAGGTATAAGATGCCTAGGATGGTTACTAAAATTGAAGGTAGAGGTAATGGCATCAAGACTAATGTAGTTAACATGGTGGACATTGCAAAAGCCTTGGGCAGACCTGCTGCTTACACTACCAAGTACTTCGGATGTGAGCTTGGCGCACAATCCAAGTTTGATGAGAAAACAGGTATTGCTCTTGTAAATGGAGCACATGAGACGGCGAAGTTGGCTGGTCTTCTCGAGAACTTCATCAAGAAATATGTTCAATGCTATGGTTGTGGAAACCCTGAAACTGAGATAATCATCACTAAGACGCAGATGATCAATATGAAATGTGCTGCTTGTGGATTCATATCTGATGTTGATATGAGAGATAAACTCACTACATTTATTCTGAAGAATCCTCCTGAACAGAAGAAGGGATCGAAAGACAAGAAAGCAATGAGGCGGGCTGAGAAGGAACGGCTTAAGGAAGGAGAGGCTGCTGATGAGGAACTGAAGAAAATAAAGAAGGATTCCCTGAAGAAGAAAAGCACTAGTGGTAATGCAAAGGAAGCTGTAACCAAAGTTCCAAGCAAGAAGAAGAGTAACGGCTCTGATGAGGATCATTCTCCAACTCGTAGCCATGCCGTTGAGAATGACCAAGCTGCTGATGACGACGACGACGACGACGATGTACAGTGGCAAACAGACACATCTTTGGAGGCAGCCAAGCAGCGTATTCAGGAGCAGTTGAGTGCTGTCACTGCTGATATGGTCATGCTCTCTACAACTGAAGAGACAAAGTCGTCAAAGAAGTCCCCCGAACACGAGAAGTCTGTGGTCAACGGAAGCAAGAATGGAAATGGAGTGTCTACCCATGGTGCCCTTGTTCATGAGATCAAAGATTATTTGAAGAAAAGTCCCTCTGCAGCCGATCTTAAATCATTCTTGGAGTCGCTTACTGGAACTCACCAAGAGACTGCGAATGCCTTAGTTGAGGCTCTATTTGAAGGAGTTGGTAAAGGGTTCTCAAAGGAGGTAGTGAAAAAGAAGAAGTTCCTGGTTGCAGTTGCAGCAGCTCAGGAGGAGGGATCACAGATAGTTCTGTTGCGTTCCATCGAATCTTTCTGTCTCAACACGAGTCCGGAGGCGGCTAAGGAGATCGCACTGGTCCTGAAATCGTTATACGATGGGGATGTAATAGAGGAGGAATTCGTGCTAGAGTGGTACCAGCAGGGACTGGCAGGAGCTAACAAAGGTAGCCAAATCTGGAAGCATGTAAAGCCGTTCATCGAGTGGCTCCAGAACGCAGAGTCGGAGACGGAAGAAGAGTAA

mRNA sequence

ATGGCGTTACAGAACATTGGTGCCTCAAACCGTGACGATGCCTTCTATAGGTATAAGATGCCTAGGATGGTTACTAAAATTGAAGGTAGAGGTAATGGCATCAAGACTAATGTAGTTAACATGGTGGACATTGCAAAAGCCTTGGGCAGACCTGCTGCTTACACTACCAAGTACTTCGGATGTGAGCTTGGCGCACAATCCAAGTTTGATGAGAAAACAGGTATTGCTCTTGTAAATGGAGCACATGAGACGGCGAAGTTGGCTGGTCTTCTCGAGAACTTCATCAAGAAATATGTTCAATGCTATGGTTGTGGAAACCCTGAAACTGAGATAATCATCACTAAGACGCAGATGATCAATATGAAATGTGCTGCTTGTGGATTCATATCTGATGTTGATATGAGAGATAAACTCACTACATTTATTCTGAAGAATCCTCCTGAACAGAAGAAGGGATCGAAAGACAAGAAAGCAATGAGGCGGGCTGAGAAGGAACGGCTTAAGGAAGGAGAGGCTGCTGATGAGGAACTGAAGAAAATAAAGAAGGATTCCCTGAAGAAGAAAAGCACTAGTGGTAATGCAAAGGAAGCTGTAACCAAAGTTCCAAGCAAGAAGAAGAGTAACGGCTCTGATGAGGATCATTCTCCAACTCGTAGCCATGCCGTTGAGAATGACCAAGCTGCTGATGACGACGACGACGACGACGATGTACAGTGGCAAACAGACACATCTTTGGAGGCAGCCAAGCAGCGTATTCAGGAGCAGTTGAGTGCTGTCACTGCTGATATGGTCATGCTCTCTACAACTGAAGAGACAAAGTCGTCAAAGAAGTCCCCCGAACACGAGAAGTCTGTGGTCAACGGAAGCAAGAATGGAAATGGAGTGTCTACCCATGGTGCCCTTGTTCATGAGATCAAAGATTATTTGAAGAAAAGTCCCTCTGCAGCCGATCTTAAATCATTCTTGGAGTCGCTTACTGGAACTCACCAAGAGACTGCGAATGCCTTAGTTGAGGCTCTATTTGAAGGAGTTGGTAAAGGGTTCTCAAAGGAGGTAGTGAAAAAGAAGAAGTTCCTGGTTGCAGTTGCAGCAGCTCAGGAGGAGGGATCACAGATAGTTCTGTTGCGTTCCATCGAATCTTTCTGTCTCAACACGAGTCCGGAGGCGGCTAAGGAGATCGCACTGGTCCTGAAATCGTTATACGATGGGGATGTAATAGAGGAGGAATTCGTGCTAGAGTGGTACCAGCAGGGACTGGCAGGAGCTAACAAAGGTAGCCAAATCTGGAAGCATGTAAAGCCGTTCATCGAGTGGCTCCAGAACGCAGAGTCGGAGACGGAAGAAGAGTAA

Coding sequence (CDS)

ATGGCGTTACAGAACATTGGTGCCTCAAACCGTGACGATGCCTTCTATAGGTATAAGATGCCTAGGATGGTTACTAAAATTGAAGGTAGAGGTAATGGCATCAAGACTAATGTAGTTAACATGGTGGACATTGCAAAAGCCTTGGGCAGACCTGCTGCTTACACTACCAAGTACTTCGGATGTGAGCTTGGCGCACAATCCAAGTTTGATGAGAAAACAGGTATTGCTCTTGTAAATGGAGCACATGAGACGGCGAAGTTGGCTGGTCTTCTCGAGAACTTCATCAAGAAATATGTTCAATGCTATGGTTGTGGAAACCCTGAAACTGAGATAATCATCACTAAGACGCAGATGATCAATATGAAATGTGCTGCTTGTGGATTCATATCTGATGTTGATATGAGAGATAAACTCACTACATTTATTCTGAAGAATCCTCCTGAACAGAAGAAGGGATCGAAAGACAAGAAAGCAATGAGGCGGGCTGAGAAGGAACGGCTTAAGGAAGGAGAGGCTGCTGATGAGGAACTGAAGAAAATAAAGAAGGATTCCCTGAAGAAGAAAAGCACTAGTGGTAATGCAAAGGAAGCTGTAACCAAAGTTCCAAGCAAGAAGAAGAGTAACGGCTCTGATGAGGATCATTCTCCAACTCGTAGCCATGCCGTTGAGAATGACCAAGCTGCTGATGACGACGACGACGACGACGATGTACAGTGGCAAACAGACACATCTTTGGAGGCAGCCAAGCAGCGTATTCAGGAGCAGTTGAGTGCTGTCACTGCTGATATGGTCATGCTCTCTACAACTGAAGAGACAAAGTCGTCAAAGAAGTCCCCCGAACACGAGAAGTCTGTGGTCAACGGAAGCAAGAATGGAAATGGAGTGTCTACCCATGGTGCCCTTGTTCATGAGATCAAAGATTATTTGAAGAAAAGTCCCTCTGCAGCCGATCTTAAATCATTCTTGGAGTCGCTTACTGGAACTCACCAAGAGACTGCGAATGCCTTAGTTGAGGCTCTATTTGAAGGAGTTGGTAAAGGGTTCTCAAAGGAGGTAGTGAAAAAGAAGAAGTTCCTGGTTGCAGTTGCAGCAGCTCAGGAGGAGGGATCACAGATAGTTCTGTTGCGTTCCATCGAATCTTTCTGTCTCAACACGAGTCCGGAGGCGGCTAAGGAGATCGCACTGGTCCTGAAATCGTTATACGATGGGGATGTAATAGAGGAGGAATTCGTGCTAGAGTGGTACCAGCAGGGACTGGCAGGAGCTAACAAAGGTAGCCAAATCTGGAAGCATGTAAAGCCGTTCATCGAGTGGCTCCAGAACGCAGAGTCGGAGACGGAAGAAGAGTAA

Protein sequence

MALQNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFGCELGAQSKFDEKTGIALVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMINMKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEELKKIKKDSLKKKSTSGNAKEAVTKVPSKKKSNGSDEDHSPTRSHAVENDQAADDDDDDDDVQWQTDTSLEAAKQRIQEQLSAVTADMVMLSTTEETKSSKKSPEHEKSVVNGSKNGNGVSTHGALVHEIKDYLKKSPSAADLKSFLESLTGTHQETANALVEALFEGVGKGFSKEVVKKKKFLVAVAAAQEEGSQIVLLRSIESFCLNTSPEAAKEIALVLKSLYDGDVIEEEFVLEWYQQGLAGANKGSQIWKHVKPFIEWLQNAESETEEE

Homology

[Close]

BLAST of Carg09228-RA vs. NCBI nr
Match: XP_023546001.1 (eukaryotic translation initiation factor 5-like [Cucurbita pepo subsp. pepo] >XP_023546002.1 eukaryotic translation initiation factor 5-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 686.4 bits (1770), Expect = 6.4e-194
Identity = 444/449 (98.89%), Postives = 444/449 (98.89%), Query Frame = 0


Query: 1   MALQNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFG 60
           MALQNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFG
Sbjct: 1   MALQNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFG 60

Query: 61  CELGAQSKFDEKTGIALVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIN 120
           CELGAQSKFDEKTGIALVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIN
Sbjct: 61  CELGAQSKFDEKTGIALVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIN 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERXXXXXXXXXXXXXX 180
           MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKER XXXXXXXXXXXXX
Sbjct: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLXXXXXXXXXXXXX 180

Query: 181 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXHAVXXXXXXXXXXXXXXXQWQ 240
           XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX   XXXXXXXXXXXXXXXQWQ
Sbjct: 181 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQWQ 240

Query: 241 TDTSLEAAKQRIQEQLSAVTADMVMLSTTEETKSSKKSPEHEKSVVNGSKNGNGVSTHGA 300
           TDTSLEAAKQRIQEQLSAVTADMVMLSTTEETKSSKKSPEHEKSVVNGSKNGNGVSTHGA
Sbjct: 241 TDTSLEAAKQRIQEQLSAVTADMVMLSTTEETKSSKKSPEHEKSVVNGSKNGNGVSTHGA 300

Query: 301 LVHEIKDYLKKSPSAADLKSFLESLTGTHQETANALVEALFEGVGKGFSKEVVKXXXXXX 360
           LVHEIKDYLKKSPSAADLKSFLESLTGTHQETANALVEALFEGVGKGFSKEVVKXXXXXX
Sbjct: 301 LVHEIKDYLKKSPSAADLKSFLESLTGTHQETANALVEALFEGVGKGFSKEVVKXXXXXX 360

Query: 361 XXXXXXEEGSQIVLLRSIESFCLNTSPEAAKEIALVLKSLYDGDVIEEEFVLEWYQQGLA 420
           XXXXXXEEGSQIVLLRSIESFCLNTSPEAAKEIALVLKSLYDGDVIEEE VLEWYQQGLA
Sbjct: 361 XXXXXXEEGSQIVLLRSIESFCLNTSPEAAKEIALVLKSLYDGDVIEEEIVLEWYQQGLA 420

Query: 421 GANKGSQIWKHVKPFIEWLQNAESETEEE 450
           GANKGSQIWKHVKPFIEWLQNAESETEEE
Sbjct: 421 GANKGSQIWKHVKPFIEWLQNAESETEEE 449

[Close]

BLAST of Carg09228-RA vs. NCBI nr
Match: XP_022962417.1 (probable eukaryotic translation initiation factor 5-1 [Cucurbita moschata] >XP_022962418.1 probable eukaryotic translation initiation factor 5-1 [Cucurbita moschata])

HSP 1 Score: 681.0 bits (1756), Expect = 2.7e-192
Identity = 442/449 (98.44%), Postives = 442/449 (98.44%), Query Frame = 0


Query: 1   MALQNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFG 60
           MALQNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFG
Sbjct: 1   MALQNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFG 60

Query: 61  CELGAQSKFDEKTGIALVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIN 120
           CELGAQSKFDEKTGIALVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIN
Sbjct: 61  CELGAQSKFDEKTGIALVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIN 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERXXXXXXXXXXXXXX 180
           MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKER  XXXXXXXXXXXX
Sbjct: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKER-LXXXXXXXXXXXX 180

Query: 181 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXHAVXXXXXXXXXXXXXXXQWQ 240
           XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX   XXXXXXXXXXXXXXXQWQ
Sbjct: 181 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQWQ 240

Query: 241 TDTSLEAAKQRIQEQLSAVTADMVMLSTTEETKSSKKSPEHEKSVVNGSKNGNGVSTHGA 300
           TDTSLEAAKQRIQEQLSAVTADMVMLSTTEETKSSKKSPEHEKSVVNGSKNGNGVSTHGA
Sbjct: 241 TDTSLEAAKQRIQEQLSAVTADMVMLSTTEETKSSKKSPEHEKSVVNGSKNGNGVSTHGA 300

Query: 301 LVHEIKDYLKKSPSAADLKSFLESLTGTHQETANALVEALFEGVGKGFSKEVVKXXXXXX 360
           LVHEIKDYLKKSPSAADLKSFLESLTGTHQETANALVEALFEGVGKGFSKEVVKXXXXXX
Sbjct: 301 LVHEIKDYLKKSPSAADLKSFLESLTGTHQETANALVEALFEGVGKGFSKEVVKXXXXXX 360

Query: 361 XXXXXXEEGSQIVLLRSIESFCLNTSPEAAKEIALVLKSLYDGDVIEEEFVLEWYQQGLA 420
           XXXXXXEEGSQIVLLRSIESFCLNT PE AKEIALVLKSLYDGDVIEEEFVLEWYQQGLA
Sbjct: 361 XXXXXXEEGSQIVLLRSIESFCLNTCPEVAKEIALVLKSLYDGDVIEEEFVLEWYQQGLA 420

Query: 421 GANKGSQIWKHVKPFIEWLQNAESETEEE 450
           GANKGSQIWKHVKPFIEWLQNAESETEEE
Sbjct: 421 GANKGSQIWKHVKPFIEWLQNAESETEEE 448

[Close]

BLAST of Carg09228-RA vs. NCBI nr
Match: XP_022997142.1 (probable eukaryotic translation initiation factor 5-1 [Cucurbita maxima] >XP_022997143.1 probable eukaryotic translation initiation factor 5-1 [Cucurbita maxima])

HSP 1 Score: 675.6 bits (1742), Expect = 1.1e-190
Identity = 439/449 (97.77%), Postives = 443/449 (98.66%), Query Frame = 0


Query: 1   MALQNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFG 60
           MALQNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFG
Sbjct: 1   MALQNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFG 60

Query: 61  CELGAQSKFDEKTGIALVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIN 120
           CELGAQSKFDEKTGIALVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIN
Sbjct: 61  CELGAQSKFDEKTGIALVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIN 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERXXXXXXXXXXXXXX 180
           MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKER  XXXXXXXXXXXX
Sbjct: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKER-LXXXXXXXXXXXX 180

Query: 181 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXHAVXXXXXXXXXXXXXXXQWQ 240
           XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX   XXXXXXXXXXXXXXXQWQ
Sbjct: 181 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQWQ 240

Query: 241 TDTSLEAAKQRIQEQLSAVTADMVMLSTTEETKSSKKSPEHEKSVVNGSKNGNGVSTHGA 300
           TDTSLEAAKQRIQEQLSAVTADMVMLSTTEETKSSKKSPEHEKSVVNGSKNGN VS+HGA
Sbjct: 241 TDTSLEAAKQRIQEQLSAVTADMVMLSTTEETKSSKKSPEHEKSVVNGSKNGNRVSSHGA 300

Query: 301 LVHEIKDYLKKSPSAADLKSFLESLTGTHQETANALVEALFEGVGKGFSKEVVKXXXXXX 360
           LV+EIKDYLK+SPSAADLKSFLESLTGTHQETANAL+EALFEGVGKGFSKEVVKXXXXXX
Sbjct: 301 LVNEIKDYLKRSPSAADLKSFLESLTGTHQETANALIEALFEGVGKGFSKEVVKXXXXXX 360

Query: 361 XXXXXXEEGSQIVLLRSIESFCLNTSPEAAKEIALVLKSLYDGDVIEEEFVLEWYQQGLA 420
           XXXXXXEEGSQIVLLRSIESFCLNTSPEAAKEIALVLKSLYDGDVIEEEFVLEWYQQGLA
Sbjct: 361 XXXXXXEEGSQIVLLRSIESFCLNTSPEAAKEIALVLKSLYDGDVIEEEFVLEWYQQGLA 420

Query: 421 GANKGSQIWKHVKPFIEWLQNAESETEEE 450
           GANKGSQIWKHVKPFIEWLQNAESETEEE
Sbjct: 421 GANKGSQIWKHVKPFIEWLQNAESETEEE 448

[Close]

BLAST of Carg09228-RA vs. NCBI nr
Match: XP_022131574.1 (eukaryotic translation initiation factor 5-like [Momordica charantia] >XP_022131575.1 eukaryotic translation initiation factor 5-like [Momordica charantia] >XP_022131576.1 eukaryotic translation initiation factor 5-like [Momordica charantia])

HSP 1 Score: 574.7 bits (1480), Expect = 2.7e-160
Identity = 387/449 (86.19%), Postives = 404/449 (89.98%), Query Frame = 0


Query: 1   MALQNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFG 60
           MALQNIGASNRDDAFYRYKMP+MVTKIEGRGNGIKTNVVNMV+IAKALGRPA+YTTKYFG
Sbjct: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60

Query: 61  CELGAQSKFDEKTGIALVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIN 120
           CELGAQSKFDEKTG +LVNGAH+TAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMI 
Sbjct: 61  CELGAQSKFDEKTGTSLVNGAHDTAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERXXXXXXXXXXXXXX 180
           MKCAACGFISDVDMRDKLTTFILKNP                    XXXXXXXXXXXXXX
Sbjct: 121 MKCAACGFISDVDMRDKLTTFILKNPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 180

Query: 181 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXHAVXXXXXXXXXXXXXXXQWQ 240
           XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX   XXXXXXXXXXXXXXXQWQ
Sbjct: 181 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQWQ 240

Query: 241 TDTSLEAAKQRIQEQLSAVTADMVMLSTTEETKSSKKSPEHEKSVVNGSKNGNGVSTHGA 300
           TDTSLEAAKQRIQEQLSAVTADMVMLSTTEE KSSKKS E+EKS VNG+KNGNG+S HGA
Sbjct: 241 TDTSLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSTENEKSQVNGNKNGNGISAHGA 300

Query: 301 LVHEIKDYLKKSPSAADLKSFLESLTGTHQETANALVEALFEGVGKGFSKEVVKXXXXXX 360
           LVHEIKDYLKK  SAA+LKSFLESL+GT QE  NALVE LF+GVGKGFSKEV+K  XXXX
Sbjct: 301 LVHEIKDYLKKGSSAAELKSFLESLSGTRQEVVNALVECLFDGVGKGFSKEVLK-KXXXX 360

Query: 361 XXXXXXEEGSQIVLLRSIESFCLNTSPEAAKEIALVLKSLYDGDVIEEEFVLEWYQQGLA 420
           XXXXXX    QI+LLR+IES CLNT+PEA KE+ALVLKSLYDGDV+EEEFVLEWYQQGLA
Sbjct: 361 XXXXXXXXXXQIILLRAIESLCLNTNPEAGKEVALVLKSLYDGDVLEEEFVLEWYQQGLA 420

Query: 421 GANKGSQIWKHVKPFIEWLQNAESETEEE 450
           G NK SQIWKHVKPFIEWLQNAESETEEE
Sbjct: 421 GENKSSQIWKHVKPFIEWLQNAESETEEE 448

[Close]

BLAST of Carg09228-RA vs. NCBI nr
Match: XP_023536674.1 (probable eukaryotic translation initiation factor 5-1 [Cucurbita pepo subsp. pepo] >XP_023536675.1 probable eukaryotic translation initiation factor 5-1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 555.8 bits (1431), Expect = 1.3e-154
Identity = 373/449 (83.07%), Postives = 391/449 (87.08%), Query Frame = 0


Query: 1   MALQNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFG 60
           MALQNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMV+IAKALGRPA+YTTKYFG
Sbjct: 1   MALQNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60

Query: 61  CELGAQSKFDEKTGIALVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIN 120
           CELGAQSKFDEKTG +LVNGAH+TAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMI 
Sbjct: 61  CELGAQSKFDEKTGTSLVNGAHDTAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERXXXXXXXXXXXXXX 180
           MKCAACGFISDVDMRDK+TTFILKN                     XXXXXXXXXXXXXX
Sbjct: 121 MKCAACGFISDVDMRDKITTFILKNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 180

Query: 181 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXHAVXXXXXXXXXXXXXXXQWQ 240
           XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX   XXXXXXXXXXXXXXXQWQ
Sbjct: 181 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQWQ 240

Query: 241 TDTSLEAAKQRIQEQLSAVTADMVMLSTTEETKSSKKSPEHEKSVVNGSKNGNGVSTHGA 300
           TDTSLEAAKQRIQEQLSAVTADMVMLS TEET SSKK  EHEKS +NG+KNGNGVSTH A
Sbjct: 241 TDTSLEAAKQRIQEQLSAVTADMVMLS-TEETTSSKKPSEHEKSSINGNKNGNGVSTHVA 300

Query: 301 LVHEIKDYLKKSPSAADLKSFLESLTGTHQETANALVEALFEGVGKGFSKEVVKXXXXXX 360
           LV+EIKDYLKK  SAA+LKSFL SL+GT QE  NALVEALF+GVGKGFSKEV+K      
Sbjct: 301 LVNEIKDYLKKGSSAANLKSFLGSLSGTPQEIVNALVEALFDGVGKGFSKEVMKKKNFLA 360

Query: 361 XXXXXXEEGSQIVLLRSIESFCLNTSPEAAKEIALVLKSLYDGDVIEEEFVLEWYQQGLA 420
                  EGSQI LLR+IESFCLNTS EAAKE+ALVLKSLYD ++IEEEFVLEWYQ+G+ 
Sbjct: 361 AAVAL--EGSQIALLRAIESFCLNTSSEAAKEVALVLKSLYDSNLIEEEFVLEWYQKGVV 420

Query: 421 GANKGSQIWKHVKPFIEWLQNAESETEEE 450
           GANK S IW HVKPFIEWLQNAESETEEE
Sbjct: 421 GANKSSPIWNHVKPFIEWLQNAESETEEE 446

[Close]

BLAST of Carg09228-RA vs. TAIR10
Match: AT1G77840.1 (Translation initiation factor IF2/IF5)

HSP 1 Score: 473.4 bits (1217), Expect = 1.6e-133
Identity = 284/450 (63.11%), Postives = 331/450 (73.56%), Query Frame = 0


Query: 1   MALQNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFG 60
           MALQNIGASNR+DAFYRYKMP+MVTK EG+GNGIKTN++N V+IAKAL RP +YTTKYFG
Sbjct: 1   MALQNIGASNRNDAFYRYKMPKMVTKTEGKGNGIKTNIINNVEIAKALARPPSYTTKYFG 60

Query: 61  CELGAQSKFDEKTGIALVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIN 120
           CELGAQSKFDEKTG +LVNGAH T+KLAGLLENFIKK+VQCYGCGNPETEIIITKTQM+N
Sbjct: 61  CELGAQSKFDEKTGTSLVNGAHNTSKLAGLLENFIKKFVQCYGCGNPETEIIITKTQMVN 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERXXXXXXXXXXXXXX 180
           +KCAACGFIS+VDMRDKLT FILKNPPEQKK SKDKKAMR+AEKER              
Sbjct: 121 LKCAACGFISEVDMRDKLTNFILKNPPEQKKVSKDKKAMRKAEKER-----------LKE 180

Query: 181 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXHAVXXXXXXXXXXXXXXXQWQ 240
                  XXXXXXXXXXXXXXX                 H       XXXXXXXXX +WQ
Sbjct: 181 GELADEEXXXXXXXXXXXXXXXDSKTSKNHSSDEDISPKHDENALEVXXXXXXXXXVEWQ 240

Query: 241 TDTSLEAAKQRIQEQLSAVTADMVMLSTTEETKSSKKSPEHEKSVVNGSKNGNGVSTHGA 300
           TDTS EAA++R+ EQLSA TA+MVMLS  E     KK+P+  KS  N  K  N       
Sbjct: 241 TDTSREAAEKRMMEQLSAKTAEMVMLSAME--VEEKKAPK-SKSNGNVVKTENPPPQEKN 300

Query: 301 LVHEIKDYLKKSPSAADLKSFLESLTGTHQETANALVEALFEGVGKGFSKEVVKXXXXXX 360
           LV ++K+YLKK    + LKSF+ SL+   Q+  +AL  ALF+GVGKGF+KEV K      
Sbjct: 301 LVQDMKEYLKKGSPISALKSFISSLSEPPQDIMDALFNALFDGVGKGFAKEVTKKKNYLA 360

Query: 361 XXXXXXEEGSQIVLLRSIESFC-LNTSPEAAKEIALVLKSLYDGDVIEEEFVLEWYQQGL 420
                 E+GSQ+ LL SI +FC  N + EA KE+ALVLK+LYD D+IEEE VL+WY++GL
Sbjct: 361 AAATMQEDGSQMHLLNSIGTFCGKNGNEEALKEVALVLKALYDQDIIEEEVVLDWYEKGL 420

Query: 421 AGANKGSQIWKHVKPFIEWLQNAESETEEE 450
            GA+K S +WK+VKPF+EWLQ+AESE+EEE
Sbjct: 421 TGADKSSPVWKNVKPFVEWLQSAESESEEE 436

[Close]

BLAST of Carg09228-RA vs. TAIR10
Match: AT1G36730.1 (Translation initiation factor IF2/IF5)

HSP 1 Score: 408.7 bits (1049), Expect = 4.7e-114
Identity = 318/458 (69.43%), Postives = 359/458 (78.38%), Query Frame = 0


Query: 1   MALQNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFG 60
           MALQNIGASNRDDAFYRYKMPRM+TKIEGRGNGIKTNVVNMV+IAKALGRPAAYTTKYFG
Sbjct: 1   MALQNIGASNRDDAFYRYKMPRMMTKIEGRGNGIKTNVVNMVEIAKALGRPAAYTTKYFG 60

Query: 61  CELGAQSKFDEKTGIALVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIN 120
           CELGAQSKFDEK G +LVNGAH+T+KLAGLLENFIKKYVQCYGCGNPETEI+ITKTQM+ 
Sbjct: 61  CELGAQSKFDEKNGTSLVNGAHDTSKLAGLLENFIKKYVQCYGCGNPETEILITKTQMLQ 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERXXXXXXXXXXXXXX 180
           +KCAACGF+SDVDMRDKLT+FILK                      XXXXXXXXXXXXXX
Sbjct: 121 LKCAACGFLSDVDMRDKLTSFILK---------XXXXXXXXXXXXXXXXXXXXXXXXXXX 180

Query: 181 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXHAVXXXXXXXXXXXXXXXQWQ 240
           XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX   XXXXXXXXXXXXXXX WQ
Sbjct: 181 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXWQ 240

Query: 241 TDTSLEAAKQRIQEQLSAVTADMVMLSTTEETK---SSKKSPEHEKSVVNGSKNGNG--- 300
           TDTS EAA++R++EQLSAVTA+MVMLST EE K     KK+PE         +NGN    
Sbjct: 241 TDTSREAAEKRMKEQLSAVTAEMVMLSTVEEKKPVAEVKKAPEQV------HENGNSKIP 300

Query: 301 VSTHGALVHEIKDYLKKSPSAADLKSFLESLTGTHQETANALVEALFEGVGKGFSKEVVK 360
            + H  LV+EIK+ L    S   LK+ L S +   QE  +AL  ALF G GKGF+KEV+ 
Sbjct: 301 ENAHEKLVNEIKELLSSGSSPTQLKTALASNSANPQEKMDALFSALFGGTGKGFAKEVIX 360

Query: 361 XXXXXXXXXXXXEEG---SQIVLLRSIESFCLNTSPEAAKEIALVLKSLYDGDVIEEEFV 420
           XXXXXXXXXXXX      +Q+ LL  IESFC+  S EAAKE+ALV+K LYD D+++E+ +
Sbjct: 361 XXXXXXXXXXXXXXAGAPAQMGLLNGIESFCMKASAEAAKEVALVIKGLYDEDILDEDVI 420

Query: 421 LEWYQQGLAGANKGSQIWKHVKPFIEWLQNAESETEEE 450
           +EWY +G+    K S + K+V PFIEWLQNAESE+EEE
Sbjct: 421 VEWYNKGV----KSSPVLKNVTPFIEWLQNAESESEEE 439

[Close]

BLAST of Carg09228-RA vs. TAIR10
Match: AT5G20920.1 (eukaryotic translation initiation factor 2 beta subunit)

HSP 1 Score: 56.6 bits (135), Expect = 4.5e-08
Identity = 28/93 (30.11%), Postives = 46/93 (49.46%), Query Frame = 0


Query: 35  KTNVVNMVDIAKALGRPAAYTTKYFGCELGAQSKFDEKTGIALVNGAHETAKLAGLLENF 94
           KT  VN +D+ K + R   +  +Y   ELG     D +  + +V G        G+L  +
Sbjct: 157 KTVFVNFMDLCKTMHRQPDHVMQYLLAELGTSGSLDGQQRL-VVKGRFAPKNFEGILRRY 216

Query: 95  IKKYVQCYGCGNPETEIIITKTQMINMKCAACG 128
           I  YV C GC +P+T I+  + ++  ++C  CG
Sbjct: 217 ITDYVICLGCKSPDT-ILSKENRLFFLRCEKCG 247

[Close]

BLAST of Carg09228-RA vs. TAIR10
Match: AT2G34970.1 (Trimeric LpxA-like enzyme)

HSP 1 Score: 46.6 bits (109), Expect = 4.7e-05
Identity = 23/73 (31.51%), Postives = 40/73 (54.79%), Query Frame = 0


Query: 371 QIVLLRSIESFCLNTSPEAAKEIALVLKSLYDGDVIEEEFVLEWYQQGLAGANKGSQIW- 430
           QI ++   E  C  +  E       +L  LYD DV++E+ +L W ++  AGA++  +++ 
Sbjct: 642 QIEVIMKFEEMCQESHKELGPLFTQILHLLYDKDVLQEDAILRW-EEEKAGADEADKVYL 701

Query: 431 KHVKPFIEWLQNA 443
           K    FI+WL+ A
Sbjct: 702 KQCDTFIQWLKEA 713

[Close]

BLAST of Carg09228-RA vs. TAIR10
Match: AT4G18300.1 (Trimeric LpxA-like enzyme)

HSP 1 Score: 46.6 bits (109), Expect = 4.7e-05
Identity = 27/81 (33.33%), Postives = 46/81 (56.79%), Query Frame = 0


Query: 370 SQIVLLRSIESFCLNTSPEAAKEIALVLKSLYDGDVIEEEFVLEWYQQGLAGANKGSQIW 429
           +QI ++   E  C   S E +   A +L  LYD DV++E+ +L W ++  AGA+   +++
Sbjct: 630 AQIEVIMKFEEMC-QESEELSPLFAKLLPFLYDKDVVQEDAILRWGEE-KAGADDCDKVY 689

Query: 430 -KHVKPFIEWLQNAESETEEE 450
               + FI+WL+ A  E EE+
Sbjct: 690 LNKCESFIKWLKEASEEEEED 708

[Close]

BLAST of Carg09228-RA vs. Swiss-Prot
Match: sp|Q9S825|IF5Z_ARATH (Probable eukaryotic translation initiation factor 5-2 OS=Arabidopsis thaliana OX=3702 GN=At1g77840 PE=1 SV=1)

HSP 1 Score: 473.4 bits (1217), Expect = 2.8e-132
Identity = 284/450 (63.11%), Postives = 331/450 (73.56%), Query Frame = 0


Query: 1   MALQNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFG 60
           MALQNIGASNR+DAFYRYKMP+MVTK EG+GNGIKTN++N V+IAKAL RP +YTTKYFG
Sbjct: 1   MALQNIGASNRNDAFYRYKMPKMVTKTEGKGNGIKTNIINNVEIAKALARPPSYTTKYFG 60

Query: 61  CELGAQSKFDEKTGIALVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIN 120
           CELGAQSKFDEKTG +LVNGAH T+KLAGLLENFIKK+VQCYGCGNPETEIIITKTQM+N
Sbjct: 61  CELGAQSKFDEKTGTSLVNGAHNTSKLAGLLENFIKKFVQCYGCGNPETEIIITKTQMVN 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERXXXXXXXXXXXXXX 180
           +KCAACGFIS+VDMRDKLT FILKNPPEQKK SKDKKAMR+AEKER              
Sbjct: 121 LKCAACGFISEVDMRDKLTNFILKNPPEQKKVSKDKKAMRKAEKER-----------LKE 180

Query: 181 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXHAVXXXXXXXXXXXXXXXQWQ 240
                  XXXXXXXXXXXXXXX                 H       XXXXXXXXX +WQ
Sbjct: 181 GELADEEXXXXXXXXXXXXXXXDSKTSKNHSSDEDISPKHDENALEVXXXXXXXXXVEWQ 240

Query: 241 TDTSLEAAKQRIQEQLSAVTADMVMLSTTEETKSSKKSPEHEKSVVNGSKNGNGVSTHGA 300
           TDTS EAA++R+ EQLSA TA+MVMLS  E     KK+P+  KS  N  K  N       
Sbjct: 241 TDTSREAAEKRMMEQLSAKTAEMVMLSAME--VEEKKAPK-SKSNGNVVKTENPPPQEKN 300

Query: 301 LVHEIKDYLKKSPSAADLKSFLESLTGTHQETANALVEALFEGVGKGFSKEVVKXXXXXX 360
           LV ++K+YLKK    + LKSF+ SL+   Q+  +AL  ALF+GVGKGF+KEV K      
Sbjct: 301 LVQDMKEYLKKGSPISALKSFISSLSEPPQDIMDALFNALFDGVGKGFAKEVTKKKNYLA 360

Query: 361 XXXXXXEEGSQIVLLRSIESFC-LNTSPEAAKEIALVLKSLYDGDVIEEEFVLEWYQQGL 420
                 E+GSQ+ LL SI +FC  N + EA KE+ALVLK+LYD D+IEEE VL+WY++GL
Sbjct: 361 AAATMQEDGSQMHLLNSIGTFCGKNGNEEALKEVALVLKALYDQDIIEEEVVLDWYEKGL 420

Query: 421 AGANKGSQIWKHVKPFIEWLQNAESETEEE 450
            GA+K S +WK+VKPF+EWLQ+AESE+EEE
Sbjct: 421 TGADKSSPVWKNVKPFVEWLQSAESESEEE 436

[Close]

BLAST of Carg09228-RA vs. Swiss-Prot
Match: sp|P48724|IF5_PHAVU (Eukaryotic translation initiation factor 5 OS=Phaseolus vulgaris OX=3885 GN=EIF5 PE=2 SV=1)

HSP 1 Score: 448.7 bits (1153), Expect = 7.4e-125
Identity = 322/451 (71.40%), Postives = 356/451 (78.94%), Query Frame = 0


Query: 1   MALQNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFG 60
           MALQNIGA N DDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAK L RPA+YTTKYFG
Sbjct: 1   MALQNIGAGNSDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKRLARPASYTTKYFG 60

Query: 61  CELGAQSKFDEKTGIALVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIN 120
           CELGAQSKFDEKTG + VNGAHETAKLAGLLE FIKKYVQCYGCGNPETEI+ITK QMI 
Sbjct: 61  CELGAQSKFDEKTGTSHVNGAHETAKLAGLLEIFIKKYVQCYGCGNPETEILITKNQMIQ 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKE-RXXXXXXXXXXXXX 180
           +KCAACGF+SDVDMRDKLTTFI+KNPPE KKGSKDKKAMRRAEKE  XXXXXXXXXXXXX
Sbjct: 121 LKCAACGFVSDVDMRDKLTTFIVKNPPEVKKGSKDKKAMRRAEKEXXXXXXXXXXXXXXX 180

Query: 181 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXHAVXXXXXXXXXXXXXXXQW 240
           XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX   XXXXXXXXXXXXXX QW
Sbjct: 181 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVQW 240

Query: 241 QTDTSLEAAKQRIQEQLSAVTADMV-MLSTTEETKSSKKSPEHEKSVVNGSKNGNGVSTH 300
            TDTSL+A++QRI+EQLSAVTADMV                         SKN      +
Sbjct: 241 LTDTSLDASRQRIKEQLSAVTADMVXXXXXXXXXXXXXXXXXXXXXXXXXSKN------Y 300

Query: 301 GALVHEIKDYLKKSPSAADLKSFLESLTGTHQETANALVEALFEGVGKGFSKEVVKXXXX 360
           G +V E+K  LKK   A++L S L +L    QE  +ALVEALFEG  KGF +E +K    
Sbjct: 301 GTVVAEVKANLKKGFGASELLSHLAALPVPAQEKMSALVEALFEGTEKGFGRETLK--KK 360

Query: 361 XXXXXXXXEEGSQIVLLRSIESFCLNTSPEAAKEIALVLKSLYDGDVIEEEFVLEWYQQG 420
                   EEGSQI+LL +IE FC   +  A KE+ALVLK+LYD DV+EEE ++ WYQ+G
Sbjct: 361 NYFAAAVAEEGSQILLLHAIEEFCCKPNSNALKEVALVLKTLYDADVLEEEAIVLWYQKG 420

Query: 421 LAGANKGSQIWKHVKPFIEWLQNAESETEEE 450
           L G NK S+IWK+ +PFI+WLQNAESE++EE
Sbjct: 421 LKGDNKNSKIWKNAQPFIDWLQNAESESDEE 443

[Close]

BLAST of Carg09228-RA vs. Swiss-Prot
Match: sp|Q9C8F1|IF5Y_ARATH (Probable eukaryotic translation initiation factor 5-1 OS=Arabidopsis thaliana OX=3702 GN=At1g36730 PE=2 SV=1)

HSP 1 Score: 408.7 bits (1049), Expect = 8.5e-113
Identity = 318/458 (69.43%), Postives = 359/458 (78.38%), Query Frame = 0


Query: 1   MALQNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFG 60
           MALQNIGASNRDDAFYRYKMPRM+TKIEGRGNGIKTNVVNMV+IAKALGRPAAYTTKYFG
Sbjct: 1   MALQNIGASNRDDAFYRYKMPRMMTKIEGRGNGIKTNVVNMVEIAKALGRPAAYTTKYFG 60

Query: 61  CELGAQSKFDEKTGIALVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIN 120
           CELGAQSKFDEK G +LVNGAH+T+KLAGLLENFIKKYVQCYGCGNPETEI+ITKTQM+ 
Sbjct: 61  CELGAQSKFDEKNGTSLVNGAHDTSKLAGLLENFIKKYVQCYGCGNPETEILITKTQMLQ 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERXXXXXXXXXXXXXX 180
           +KCAACGF+SDVDMRDKLT+FILK                      XXXXXXXXXXXXXX
Sbjct: 121 LKCAACGFLSDVDMRDKLTSFILK---------XXXXXXXXXXXXXXXXXXXXXXXXXXX 180

Query: 181 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXHAVXXXXXXXXXXXXXXXQWQ 240
           XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX   XXXXXXXXXXXXXXX WQ
Sbjct: 181 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXWQ 240

Query: 241 TDTSLEAAKQRIQEQLSAVTADMVMLSTTEETK---SSKKSPEHEKSVVNGSKNGNG--- 300
           TDTS EAA++R++EQLSAVTA+MVMLST EE K     KK+PE         +NGN    
Sbjct: 241 TDTSREAAEKRMKEQLSAVTAEMVMLSTVEEKKPVAEVKKAPEQV------HENGNSKIP 300

Query: 301 VSTHGALVHEIKDYLKKSPSAADLKSFLESLTGTHQETANALVEALFEGVGKGFSKEVVK 360
            + H  LV+EIK+ L    S   LK+ L S +   QE  +AL  ALF G GKGF+KEV+ 
Sbjct: 301 ENAHEKLVNEIKELLSSGSSPTQLKTALASNSANPQEKMDALFSALFGGTGKGFAKEVIX 360

Query: 361 XXXXXXXXXXXXEEG---SQIVLLRSIESFCLNTSPEAAKEIALVLKSLYDGDVIEEEFV 420
           XXXXXXXXXXXX      +Q+ LL  IESFC+  S EAAKE+ALV+K LYD D+++E+ +
Sbjct: 361 XXXXXXXXXXXXXXAGAPAQMGLLNGIESFCMKASAEAAKEVALVIKGLYDEDILDEDVI 420

Query: 421 LEWYQQGLAGANKGSQIWKHVKPFIEWLQNAESETEEE 450
           +EWY +G+    K S + K+V PFIEWLQNAESE+EEE
Sbjct: 421 VEWYNKGV----KSSPVLKNVTPFIEWLQNAESESEEE 439

[Close]

BLAST of Carg09228-RA vs. Swiss-Prot
Match: sp|P55876|IF5_MAIZE (Eukaryotic translation initiation factor 5 OS=Zea mays OX=4577 GN=EIF5 PE=2 SV=1)

HSP 1 Score: 407.9 bits (1047), Expect = 1.4e-112
Identity = 304/455 (66.81%), Postives = 351/455 (77.14%), Query Frame = 0


Query: 1   MALQNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFG 60
           MALQNIGASNRDDAFYRYKMPRM+TKIEGRGNGIKTNVVNMVDIAKAL RPA+YTTKYFG
Sbjct: 1   MALQNIGASNRDDAFYRYKMPRMITKIEGRGNGIKTNVVNMVDIAKALARPASYTTKYFG 60

Query: 61  CELGAQSKFDEKTGIALVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIN 120
           CELGAQSKFDEKTGI+LVNGAH+TAKLAGLLE FIKKYVQCYGCGNPETEI+I+KTQMI+
Sbjct: 61  CELGAQSKFDEKTGISLVNGAHDTAKLAGLLEVFIKKYVQCYGCGNPETEILISKTQMIS 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERXXXXXXXXXXXXXX 180
           +KCAACGF+SDVDMRDKLTTFIL                       XXXXXXXXXXXXXX
Sbjct: 121 LKCAACGFVSDVDMRDKLTTFIL----XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 180

Query: 181 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXHAVXXXXXXXXXXXXXXXQWQ 240
           XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX   XXXXXXXXXXXXXXX  Q
Sbjct: 181 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQ 240

Query: 241 TDTSLEAAKQRIQEQLSAVTADMVMLSTTEETKSSKKSPEHEKSVVNGSKNG-------- 300
           TDTS+EAAKQR+QEQLSA TA+MVMLS TEET+   K P H+    NGS           
Sbjct: 241 TDTSIEAAKQRMQEQLSAATAEMVMLS-TEETEKKMKQPTHKDGSTNGSAKEIPNDKPAV 300

Query: 301 NGVSTHGALVHEIKDYLKKSPSAADLKSFLESLTGTHQETANALVEALFEGVGKGFSKEV 360
              S +  L+ +IK  L  +P+ + LK+ L S T   Q+  NA +EALF GVGKGF+KEV
Sbjct: 301 TKPSPYEELIGDIKASLGSAPTPSQLKAVLASSTLPPQDVMNAPLEALFGGVGKGFTKEV 360

Query: 361 VKXXXXXXXXXXXXEEGSQIVLLRSIESFCLNTSPEAAKEIALVLKSLYDGDVIEEEFVL 420
           VK            +EG+Q +L+++IE+F    +PEA KE+ +VLK+LYDGD+++EE ++
Sbjct: 361 VK--NKKYLAVAVPDEGAQTLLVQAIEAFGGKCNPEALKEVPVVLKALYDGDILDEETIV 420

Query: 421 EWYQQGLAGANKGSQIWKHVKPFIEWLQNAESETE 448
           +WY   +A A K SQ+ K+ KPF+EWLQ+AES+ E
Sbjct: 421 DWYNDAVA-AGKDSQVVKNAKPFVEWLQSAESDEE 447

[Close]

BLAST of Carg09228-RA vs. Swiss-Prot
Match: sp|P55010|IF5_HUMAN (Eukaryotic translation initiation factor 5 OS=Homo sapiens OX=9606 GN=EIF5 PE=1 SV=2)

HSP 1 Score: 176.8 bits (447), Expect = 5.4e-43
Identity = 82/137 (59.85%), Postives = 100/137 (72.99%), Query Frame = 0


Query: 13  DAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFGCELGAQSKFDEK 72
           D FYRYKMPR++ K+EG+GNGIKT +VNMVD+AKAL RP  Y TKYFGCELGAQ++FD K
Sbjct: 11  DQFYRYKMPRLIAKVEGKGNGIKTVIVNMVDVAKALNRPPTYPTKYFGCELGAQTQFDVK 70

Query: 73  TGIALVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIIT-KTQMINMKCAACGFISD 132
               +VNG+HE  KL  +L+ FIKK+V C  C NPET++ +  K Q I   C ACG+   
Sbjct: 71  NDRYIVNGSHEANKLQDMLDGFIKKFVLCPECENPETDLHVNPKKQTIGNSCKACGYRGM 130

Query: 133 VDMRDKLTTFILKNPPE 149
           +D   KL TFILKNPPE
Sbjct: 131 LDTHHKLCTFILKNPPE 147

[Close]

BLAST of Carg09228-RA vs. TrEMBL
Match: tr|B9RJC4|B9RJC4_RICCO (Eukaryotic translation initiation factor, putative OS=Ricinus communis OX=3988 GN=RCOM_1033150 PE=4 SV=1)

HSP 1 Score: 527.3 bits (1357), Expect = 3.3e-146
Identity = 289/451 (64.08%), Postives = 318/451 (70.51%), Query Frame = 0


Query: 1   MALQNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFG 60
           MALQNIGASN DDAFYRYKMP+M+TKIEGRGNGIKTNVVNMVDIAKAL RPAAYTTKYFG
Sbjct: 1   MALQNIGASNSDDAFYRYKMPKMITKIEGRGNGIKTNVVNMVDIAKALARPAAYTTKYFG 60

Query: 61  CELGAQSKFDEKTGIALVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIN 120
            ELGAQSKFDEKTG +LVNGAH+T KLAGLLENFIKKYVQCYGCGNPETEI+ITKTQMIN
Sbjct: 61  NELGAQSKFDEKTGTSLVNGAHDTPKLAGLLENFIKKYVQCYGCGNPETEIVITKTQMIN 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKER-XXXXXXXXXXXXX 180
           +KCAACGF+SDVDMRDKLTTFILKNPPE KKG+KDKKAMRRAEKER              
Sbjct: 121 LKCAACGFVSDVDMRDKLTTFILKNPPEPKKGAKDKKAMRRAEKERLKEGEFADEEQKKH 180

Query: 181 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXHAVXXXXXXXXXXXXXXXQW 240
                                                                     QW
Sbjct: 181 KKETIKKKGPTVSSKDITKGTSSRKKGNGSDEDHSPTRSLPDENDQLTADDDEDDGDVQW 240

Query: 241 QTDTSLEAAKQRIQEQLSAVTADMVMLSTTEE-TKSSKKSPEHEKSVVNGSKNGNGVSTH 300
           QTDTSLEAAKQRIQEQLS VTADMVML+T EE  KS+KKSPE E  V       NG  + 
Sbjct: 241 QTDTSLEAAKQRIQEQLSTVTADMVMLTTDEEKAKSAKKSPEREVKVHENGSKSNG--SQ 300

Query: 301 GALVHEIKDYLKKSPSAADLKSFLESLTGTHQETANALVEALFEGVGKGFSKEVVKXXXX 360
             LVHEIK +LKK  S + LKS+L SL+GT QE  NAL  ALFEGV KGFSKEV K    
Sbjct: 301 EGLVHEIKAHLKKGSSPSQLKSYLSSLSGTSQEVMNALFAALFEGVEKGFSKEVTK--RK 360

Query: 361 XXXXXXXXEEGSQIVLLRSIESFCLNTSPEAAKEIALVLKSLYDGDVIEEEFVLEWYQQG 420
                   EEGSQIVLL +IESF    SPEA KE++LVLK LYD DV+EE+F+L WYQ+G
Sbjct: 361 KYLAAATQEEGSQIVLLHAIESFFAKLSPEATKEVSLVLKVLYDNDVLEEDFILAWYQKG 420

Query: 421 LAGANKGSQIWKHVKPFIEWLQNAESETEEE 450
           L+G+NK S +WK+VKPF+EWL+NAESE+EEE
Sbjct: 421 LSGSNKNSAVWKYVKPFVEWLENAESESEEE 447

[Close]

BLAST of Carg09228-RA vs. TrEMBL
Match: tr|A0A251LIP4|A0A251LIP4_MANES (Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_02G158200 PE=4 SV=1)

HSP 1 Score: 516.5 bits (1329), Expect = 5.8e-143
Identity = 346/450 (76.89%), Postives = 378/450 (84.00%), Query Frame = 0


Query: 1   MALQNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFG 60
           M LQNIGASN DDAFYRYKMP+M+TKIEGRGNGIKTNVVNMVDIAKAL RPA+YTTKYFG
Sbjct: 1   MDLQNIGASNSDDAFYRYKMPKMITKIEGRGNGIKTNVVNMVDIAKALARPASYTTKYFG 60

Query: 61  CELGAQSKFDEKTGIALVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIN 120
            ELGAQSKFDEKTG +LVNGAH+T KLAGLLENFIKKYVQCYGCGNPETEI+ITKTQMI 
Sbjct: 61  NELGAQSKFDEKTGTSLVNGAHDTPKLAGLLENFIKKYVQCYGCGNPETEIVITKTQMIT 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERXXXXXXXXXXXXXX 180
           +KCAACGF+SDVDMRDK+TTFILKNPP  KKGSKDKKAMRRAEKER   XXXXXXXXXXX
Sbjct: 121 LKCAACGFVSDVDMRDKITTFILKNPPVPKKGSKDKKAMRRAEKER-LKXXXXXXXXXXX 180

Query: 181 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXHAVXXXXXXXXXXXXXXXQWQ 240
           XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX   XXXXXXXXXXXXXX QWQ
Sbjct: 181 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVQWQ 240

Query: 241 TDTSLEAAKQRIQEQLSAVTADMVMLSTTEETKSSKKSPEHE-KSVVNGSKNGNGVSTHG 300
           TDTSLEAAKQRIQEQLSA TADMVMLST EE  +S KSPE + K+  NGSK+ +   T  
Sbjct: 241 TDTSLEAAKQRIQEQLSAATADMVMLSTNEEKANSAKSPERDVKAHQNGSKSND---TLE 300

Query: 301 ALVHEIKDYLKKSPSAADLKSFLESLTGTHQETANALVEALFEGVGKGFSKEVVKXXXXX 360
            LV+EIK YLKK   A+ LKSFL SL+GT QE  NAL  ALFEG+ KGF KEV K     
Sbjct: 301 GLVNEIKGYLKKGGPASQLKSFLSSLSGTPQEVINALFAALFEGLEKGFVKEVTK--KKN 360

Query: 361 XXXXXXXEEGSQIVLLRSIESFCLNTSPEAAKEIALVLKSLYDGDVIEEEFVLEWYQQGL 420
                  EEGSQ++LL ++ESFC    PE  KE+ L +K LYD DV+EEEF+LEWYQ+G+
Sbjct: 361 YLAAATQEEGSQMLLLHTVESFCCKAGPEVVKEVPLAVKVLYDNDVLEEEFILEWYQKGV 420

Query: 421 AGANKGSQIWKHVKPFIEWLQNAESETEEE 450
           +G NK S +WK+VKPFIEWLQNAESE+EEE
Sbjct: 421 SGGNKSSPVWKNVKPFIEWLQNAESESEEE 444

[Close]

BLAST of Carg09228-RA vs. TrEMBL
Match: tr|A0A2C9U0Z8|A0A2C9U0Z8_MANES (Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_18G072500 PE=4 SV=1)

HSP 1 Score: 514.6 bits (1324), Expect = 2.2e-142
Identity = 353/452 (78.10%), Postives = 383/452 (84.73%), Query Frame = 0


Query: 1   MALQNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFG 60
           MALQNIGASN DDAFYRYKMP+M+TKIEGRGNGIKTNVVNMV+IAKAL RPA+YTTKYFG
Sbjct: 1   MALQNIGASNSDDAFYRYKMPKMITKIEGRGNGIKTNVVNMVEIAKALARPASYTTKYFG 60

Query: 61  CELGAQSKFDEKTGIALVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIN 120
            ELGAQSKFDEKTG +LVNGAH+TAKLAGLLENFIKKYVQCYGCGNPETEI+ITKTQMI 
Sbjct: 61  NELGAQSKFDEKTGTSLVNGAHDTAKLAGLLENFIKKYVQCYGCGNPETEIVITKTQMIT 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKER-XXXXXXXXXXXXX 180
           +KCAACGFISDVDMRDKLTTFILK+PPE KKGSKDKKAMRRAEKER XXXXXXXXXXXXX
Sbjct: 121 LKCAACGFISDVDMRDKLTTFILKSPPEPKKGSKDKKAMRRAEKERLXXXXXXXXXXXXX 180

Query: 181 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXHAVXXXXXXXXXXXXXXXQW 240
           XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX   XXXXXXXXXXXXXX +W
Sbjct: 181 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVEW 240

Query: 241 QTDTSLEAAKQRIQEQLSAVTADMVMLSTTEE-TKSSKKSPEHE-KSVVNGSKNGNGVST 300
           QTDTSLEAAKQRIQEQLSAVTADMVMLST EE  KS KKSPE E KS  NG K+ +    
Sbjct: 241 QTDTSLEAAKQRIQEQLSAVTADMVMLSTVEEKAKSGKKSPEREAKSHQNGIKSND---P 300

Query: 301 HGALVHEIKDYLKKSPSAADLKSFLESLTGTHQETANALVEALFEGVGKGFSKEVVKXXX 360
              LV+EIK YL+    A+ LKSFL SL+GT QE  NAL  ALF GV KGFSKEV K   
Sbjct: 301 SEGLVNEIKGYLQMGSPASQLKSFLSSLSGTPQEVINALFAALFGGVEKGFSKEVTK--K 360

Query: 361 XXXXXXXXXEEGSQIVLLRSIESFCLNTSPEAAKEIALVLKSLYDGDVIEEEFVLEWYQQ 420
                    EEGSQ++LL + ESFC N SPE  KE+ L +K LYD DV+EEEF+LEWYQ+
Sbjct: 361 KNYLAAAVQEEGSQMLLLHAFESFCDNASPEVLKEVPLAVKVLYDKDVLEEEFILEWYQK 420

Query: 421 GLAGANKGSQIWKHVKPFIEWLQNAESETEEE 450
           GL+G NK S +WK++KP +EWLQNAESE+EEE
Sbjct: 421 GLSGGNKSSPVWKNMKPLVEWLQNAESESEEE 447

[Close]

BLAST of Carg09228-RA vs. TrEMBL
Match: tr|A0A164UXM5|A0A164UXM5_DAUCA (Uncharacterized protein OS=Daucus carota subsp. sativus OX=79200 GN=DCAR_023122 PE=4 SV=1)

HSP 1 Score: 513.5 bits (1321), Expect = 4.9e-142
Identity = 296/454 (65.20%), Postives = 331/454 (72.91%), Query Frame = 0


Query: 1   MALQNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFG 60
           MALQNIGA N+DDAFYRYKMPRM+TKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFG
Sbjct: 1   MALQNIGAGNKDDAFYRYKMPRMITKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFG 60

Query: 61  CELGAQSKFDEKTGIALVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIN 120
           CELGAQSKFDEKT  + VNGAH+TAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQM+ 
Sbjct: 61  CELGAQSKFDEKTATSHVNGAHDTAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMVT 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERXXXXXXXXXXXXXX 180
           +KCAACG +SDVDMRDKLTTFI+KNPPE KKG KDKKAMRRAEKER              
Sbjct: 121 LKCAACGHLSDVDMRDKLTTFIIKNPPE-KKGGKDKKAMRRAEKERIKAGEALDEEQKKL 180

Query: 181 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXHAVXXXXXXXXXXXXXXXQWQ 240
                 XXXXXXXXXXXXXXXX                                   QWQ
Sbjct: 181 KKEGVKXXXXXXXXXXXXXXXXKANVSDEDNSPAGSQVDEV-----DTVVSNDDDDIQWQ 240

Query: 241 TDTSLEAAKQRIQEQLSAVTADMVMLSTTEE-TKSSKKSP-EHEKSVVNGSKNG---NGV 300
           TDTS EAAKQRIQEQLSAVTA MVML+TT++  KS K SP   EK   NG  NG   N  
Sbjct: 241 TDTSAEAAKQRIQEQLSAVTAGMVMLNTTDDKPKSGKNSPVREEKPKGNGQSNGASANSS 300

Query: 301 STHGALVHEIKDYLKKSPSAADLKSFLESLTGTHQETANALVEALFEGVGKGFSKEVVKX 360
           + H  LV EIKDYLKK  SA+ LK FL++L+GT Q+  +A  EA+F+G GKGFSKEV K 
Sbjct: 301 TPHEKLVLEIKDYLKKGSSASKLKPFLDTLSGTSQQIVDAFFEAMFDGAGKGFSKEVTK- 360

Query: 361 XXXXXXXXXXXEEGSQIVLLRSIESFCLNTSPEAAKEIALVLKSLYDGDVIEEEFVLEWY 420
                       EGSQ+VLL ++E+FC   S EA KE+AL LK LYD DV+EEEF++EWY
Sbjct: 361 -KKNYLAAVTQNEGSQLVLLNALEAFCAKASSEAVKEVALALKVLYDSDVLEEEFIVEWY 420

Query: 421 QQGLAGANKGSQIWKHVKPFIEWLQNAESETEEE 450
           ++GL G NK S +WK+VKPF+EWLQ+AESETE+E
Sbjct: 421 EKGLKGGNKSSLVWKNVKPFVEWLQSAESETEDE 446

[Close]

BLAST of Carg09228-RA vs. TrEMBL
Match: tr|A0A067KLT2|A0A067KLT2_JATCU (Uncharacterized protein OS=Jatropha curcas OX=180498 GN=JCGZ_12026 PE=4 SV=1)

HSP 1 Score: 509.6 bits (1311), Expect = 7.1e-141
Identity = 349/452 (77.21%), Postives = 380/452 (84.07%), Query Frame = 0


Query: 1   MALQNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFG 60
           MALQNIGASN DDAFYRYKMP+M+TKIEGRGNGIKTNVVNMVDIAKAL RP +YTTKYFG
Sbjct: 1   MALQNIGASNSDDAFYRYKMPKMITKIEGRGNGIKTNVVNMVDIAKALARPPSYTTKYFG 60

Query: 61  CELGAQSKFDEKTGIALVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIN 120
            ELGAQSKFDEKTGI+LVNGAH+T KLAGLLENFIKKYVQCYGCGNPETEI+ITKTQMI 
Sbjct: 61  NELGAQSKFDEKTGISLVNGAHDTPKLAGLLENFIKKYVQCYGCGNPETEIVITKTQMIT 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKER-XXXXXXXXXXXXX 180
           +KCAACGFISDVDMRDKLTTFILKNPPE KKGSKDKKAMRRAEKER   XXXXXXXXXXX
Sbjct: 121 LKCAACGFISDVDMRDKLTTFILKNPPELKKGSKDKKAMRRAEKERLKEXXXXXXXXXXX 180

Query: 181 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXHAVXXXXXXXXXXXXXXXQW 240
           XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX   XXXXXXXXXXXXXXXQW
Sbjct: 181 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQW 240

Query: 241 QTDTSLEAAKQRIQEQLSAVTADMVMLSTTEE-TKSSKKSPEHEKSV-VNGSKNGNGVST 300
           QTDTSLEAAKQRIQEQLSAVTADMVMLST EE  KS+K+SP+ E  V  NGSK    +  
Sbjct: 241 QTDTSLEAAKQRIQEQLSAVTADMVMLSTEEEKAKSAKRSPDREIKVRENGSK---VIDP 300

Query: 301 HGALVHEIKDYLKKSPSAADLKSFLESLTGTHQETANALVEALFEGVGKGFSKEVVKXXX 360
              LV+E+K++LKK    + LKSFL SL G  QE  NAL  ALFEGV KGF+KE   XX 
Sbjct: 301 QQLLVNEVKEFLKKGSPPSQLKSFLGSLAGASQEVMNALFYALFEGVEKGFTKEAT-XXK 360

Query: 361 XXXXXXXXXEEGSQIVLLRSIESFCLNTSPEAAKEIALVLKSLYDGDVIEEEFVLEWYQQ 420
                    EEGSQ++LL +IESF    SP+  KE+AL+LK LYD DV+EEEF++EWYQ+
Sbjct: 361 NYLAAAVIEEEGSQLLLLNAIESFFCKASPDVVKEVALILKVLYDNDVLEEEFIMEWYQK 420

Query: 421 GLAGANKGSQIWKHVKPFIEWLQNAESETEEE 450
           G+ G NK S +WK  KPF+EWL+NAESETEEE
Sbjct: 421 GVNGGNKNSPVWKSAKPFVEWLENAESETEEE 448

The following BLAST results are available for this feature:

BLAST of Carg09228-RA vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023546001.1	6.4e-194	98.89	eukaryotic translation initiation factor 5-like [Cucurbita pepo subsp. pepo] >XP...	[more]
XP_022962417.1	2.7e-192	98.44	probable eukaryotic translation initiation factor 5-1 [Cucurbita moschata] >XP_0...	[more]
XP_022997142.1	1.1e-190	97.77	probable eukaryotic translation initiation factor 5-1 [Cucurbita maxima] >XP_022...	[more]
XP_022131574.1	2.7e-160	86.19	eukaryotic translation initiation factor 5-like [Momordica charantia] >XP_022131...	[more]
XP_023536674.1	1.3e-154	83.07	probable eukaryotic translation initiation factor 5-1 [Cucurbita pepo subsp. pep...	[more]

BLAST of Carg09228-RA vs. TAIR10


Match Name	E-value	Identity	Description
AT1G77840.1	1.6e-133	63.11	Translation initiation factor IF2/IF5	[more]
AT1G36730.1	4.7e-114	69.43	Translation initiation factor IF2/IF5	[more]
AT5G20920.1	4.5e-08	30.11	eukaryotic translation initiation factor 2 beta subunit	[more]
AT2G34970.1	4.7e-05	31.51	Trimeric LpxA-like enzyme	[more]
AT4G18300.1	4.7e-05	33.33	Trimeric LpxA-like enzyme	[more]

BLAST of Carg09228-RA vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q9S825\|IF5Z_ARATH	2.8e-132	63.11	Probable eukaryotic translation initiation factor 5-2 OS=Arabidopsis thaliana OX...	[more]
sp\|P48724\|IF5_PHAVU	7.4e-125	71.40	Eukaryotic translation initiation factor 5 OS=Phaseolus vulgaris OX=3885 GN=EIF5...	[more]
sp\|Q9C8F1\|IF5Y_ARATH	8.5e-113	69.43	Probable eukaryotic translation initiation factor 5-1 OS=Arabidopsis thaliana OX...	[more]
sp\|P55876\|IF5_MAIZE	1.4e-112	66.81	Eukaryotic translation initiation factor 5 OS=Zea mays OX=4577 GN=EIF5 PE=2 SV=1	[more]
sp\|P55010\|IF5_HUMAN	5.4e-43	59.85	Eukaryotic translation initiation factor 5 OS=Homo sapiens OX=9606 GN=EIF5 PE=1 ...	[more]

BLAST of Carg09228-RA vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|B9RJC4\|B9RJC4_RICCO	3.3e-146	64.08	Eukaryotic translation initiation factor, putative OS=Ricinus communis OX=3988 G...	[more]
tr\|A0A251LIP4\|A0A251LIP4_MANES	5.8e-143	76.89	Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_02G158200 PE=4 SV=...	[more]
tr\|A0A2C9U0Z8\|A0A2C9U0Z8_MANES	2.2e-142	78.10	Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_18G072500 PE=4 SV=...	[more]
tr\|A0A164UXM5\|A0A164UXM5_DAUCA	4.9e-142	65.20	Uncharacterized protein OS=Daucus carota subsp. sativus OX=79200 GN=DCAR_023122 ...	[more]
tr\|A0A067KLT2\|A0A067KLT2_JATCU	7.1e-141	77.21	Uncharacterized protein OS=Jatropha curcas OX=180498 GN=JCGZ_12026 PE=4 SV=1	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **Molecular Function**
Term	Definition
GO:0005488	binding
GO:0003743	translation initiation factor activity
GO:0005515	protein binding

Vocabulary: **Biological Process**
Term	Definition
GO:0006413	translational initiation

Vocabulary: **INTERPRO**
Term	Definition
IPR016024	ARM-type_fold
IPR016190	Transl_init_fac_IF2/IF5_Zn-bd
IPR016189	Transl_init_fac_IF2/IF5_N
IPR016021	MIF4-like_sf
IPR002735	Transl_init_fac_IF2/IF5
IPR003307	W2_domain

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006413	translational initiation
biological_process	GO:0006446	regulation of translational initiation
cellular_component	GO:0005840	ribosome
molecular_function	GO:0005488	binding
molecular_function	GO:0005515	protein binding
molecular_function	GO:0003743	translation initiation factor activity

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Carg09228	Carg09228	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg09228-RA.exon.1	Carg09228-RA.exon.1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg09228-RA.CDS.1	Carg09228-RA.CDS.1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Carg09228-RA	Carg09228-RA-protein	polypeptide

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 444..449
None	No IPR available	GENE3D	G3DSA:3.30.30.50		coord: 24..99 e-value: 7.1E-30 score: 104.8
None	No IPR available	GENE3D	G3DSA:2.20.25.350		coord: 101..145 e-value: 8.7E-18 score: 65.7
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 268..295
None	No IPR available	PANTHER	PTHR23001	EUKARYOTIC TRANSLATION INITIATION FACTOR	coord: 1..449
None	No IPR available	PANTHER	PTHR23001:SF14	EUKARYOTIC TRANSLATION INITIATION FACTOR 5-1-RELATED	coord: 1..449
None	No IPR available	CDD	cd11561	W2_eIF5	coord: 296..447 e-value: 8.46064E-36 score: 129.273
IPR003307	W2 domain	SMART	SM00515	542_3	coord: 357..445 e-value: 9.9E-28 score: 108.1
IPR003307	W2 domain	PFAM	PF02020	W2	coord: 371..449 e-value: 3.7E-19 score: 68.5
IPR003307	W2 domain	PROSITE	PS51363	W2	coord: 276..449 score: 23.839
IPR002735	Translation initiation factor IF2/IF5	SMART	SM00653	eIF2Bneu4	coord: 15..129 e-value: 3.5E-63 score: 225.9
IPR002735	Translation initiation factor IF2/IF5	PFAM	PF01873	eIF-5_eIF-2B	coord: 11..127 e-value: 1.3E-36 score: 125.0
IPR016021	MIF4G-like domain superfamily	GENE3D	G3DSA:1.25.40.180		coord: 293..449 e-value: 3.2E-27 score: 97.4
IPR016189	Translation initiation factor IF2/IF5, N-terminal	SUPERFAMILY	SSF100966	Translation initiation factor 2 beta, aIF2beta, N-terminal domain	coord: 10..101
IPR016190	Translation initiation factor IF2/IF5, zinc-binding	SUPERFAMILY	SSF75689	Zinc-binding domain of translation initiation factor 2 beta	coord: 98..133
IPR016024	Armadillo-type fold	SUPERFAMILY	SSF48371	ARM repeat	coord: 300..443