Carg08499-RA (mRNA) Silver-seed gourd

NameCarg08499-RA
TypemRNA
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionKinase-like protein
LocationCucurbita_argyrosperma_scaffold_022 : 645033 .. 647074 (+)
Sequence length1005
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTCAAATTAATCCTTATAGTCGCTTCCGCCACCATTTTTGTTCTTCTTCTAGTTTCCATCTTCTTTTTCCGCCATAAGCTCTCCAAAACAAACCACAGACACGACATCGAAAACCCATCGCCGGACAACAACCAGAAAAAACAAGACACGGCGGCAGCGACCGTGGAGGAGCTAACGACGTTTGAAGGCGGCGAAGATTTGACGATCGACGAGATTCTAGAAGCTCCGGGTGAAGTGATCGGAAAATACCACTATGGAACTCTGTACAAGGCTTCCTTACAGAGTACCCAGAGAACTACACTACTCATGTTCCTCCGCCCTGCCGCCGTGGAATCTAGTGTAGACGACGTCGTTCGGTTTCTGGGTTCGATCCGGCACCGGAATTTGGTGCCGATGTTGGGATTTTACGCCGGCGGGAGAGGCGAGAGACTTCTGGTTCATCCGTTTTATGGCAGAGGGAATCTGTCAGAGTTCATCACAGGTAAGTTTCATTTTGATTTTCTGTTTGTTTCTCGAGAATGTGAAAAGGGAAAAGAATCTGAGGGGAAATTGAGTGAATATCGGGGCAATGTCGGTAACCCTTTTCGCTTTCGGTTGCTGTCGGTTTTCCATGGGCGAATCAGAGAAGACGACGATTCCGGTTCGGCCGGCGGGCACGTTTTTTATTTGTCTTTCCTACCATATATATTTTTTAAAAAAATATTAAATTTAAACCAAAATTTTAATAGGCATTTTAAAAAATTAGAGATTAAAATATAAAATGAAAAATATCTAAAATTTTATTAAATGTTGAAGGAAAATAATTTTTTTAATGCATTATTGAGGTTGAAATTAAGATAATAAAATTAAAAGAAAAATTAAAAATTCTGTCCACCTCATATATTAGCTATAGCATTGATATTTTTTCTTTAGATTTTTTTCTGTATTTATTTATTTATTTATTTCTCTTTGTTAACATATTCAACTTTAATTCTCATATTATCTATGTTTTATTTATTTATTTATTTATATTTAAAATGTAAATTTTGTAGGTCATCTAAACATTTTAAATTAGTGGATAATAATTATTTTATTTATTTTTTTATTTTCAGTATTTATTTATAAAAAATTTATATTTTAGAAATCCCTAAAAATTTTCTAAACAATCATTTGTATTCCAATTGTGCTGTTTCTAAAAATTTTCTAAAAATTTATATTTCATTTAAAACTTTATTATACGTTAAATTGGAGAATATATTCCTCTTCTTTATTTATTTTTCTTTTTTTGAAATTAAATAGGAAATATATTCCAATTGTGCTGTTTGTAAATGGAGAATATATATATATATATATTTTTTATAAATAAAAGGAAATTAACCTTAAAAGACAATGTTTAAGATTTAATTTATTGAAAATATCGGAAAGTAAAATTTAAATAAAATAAACCGATTTTTAAAAATTATTAAATAAATATTTATTTTAAGAAGGGGAAAAGCTAAGGTACAACAGCAGTTATGATAATTTCTTGTGATTGAACAGTGGGAGAATCGGAATCAAAAAATTGGGAAATCATCTACAAAATCTCTCTCGGAATCGCGAAAGCCCTAGATCATCTCCACCATGGATTACCAAAACCAGTACCGCACGGAAATCTCAAGCCCAAGAACATTTTTCTCGATCCAGATTTCGAGCCGTACGTCTCCGATTTCGGCCTCCATCTCCTCCTCAACGCCGCCGCAACTCGAGAATTGCTCCAAGCTTCCTCCGCCTCCGGCTACAACGCGCCGGAACTGGTCAGGATGACTGACGCCGACGAACGGACCGACGTCTACAGCTACGGCAAGATCTTACTCGAGTTGCTCTCCGCCAAAGATACAATGAATCTAACTGCGGTTGCTTGTGGCGGAGACGGCGGCGATTGCAGTGTTTCGAAAGAGCGGAATCTCCAATGCCTTTTTCAGATCGGCGAGGCTTGTTGTTGTTCTTCGCCGATTCGGCGGCCGGATTTCAAGGAAGTTATTGAGAAGATTGAAGAAATCGGAAGGATTGAGATGGATTGA

mRNA sequence

ATGCTCAAATTAATCCTTATAGTCGCTTCCGCCACCATTTTTGTTCTTCTTCTAGTTTCCATCTTCTTTTTCCGCCATAAGCTCTCCAAAACAAACCACAGACACGACATCGAAAACCCATCGCCGGACAACAACCAGAAAAAACAAGACACGGCGGCAGCGACCGTGGAGGAGCTAACGACGTTTGAAGGCGGCGAAGATTTGACGATCGACGAGATTCTAGAAGCTCCGGGTGAAGTGATCGGAAAATACCACTATGGAACTCTGTACAAGGCTTCCTTACAGAGTACCCAGAGAACTACACTACTCATGTTCCTCCGCCCTGCCGCCGTGGAATCTAGTGTAGACGACGTCGTTCGGTTTCTGGGTTCGATCCGGCACCGGAATTTGGTGCCGATGTTGGGATTTTACGCCGGCGGGAGAGGCGAGAGACTTCTGGTTCATCCGTTTTATGGCAGAGGGAATCTGTCAGAGTTCATCACAGTGGGAGAATCGGAATCAAAAAATTGGGAAATCATCTACAAAATCTCTCTCGGAATCGCGAAAGCCCTAGATCATCTCCACCATGGATTACCAAAACCAGTACCGCACGGAAATCTCAAGCCCAAGAACATTTTTCTCGATCCAGATTTCGAGCCGTACGTCTCCGATTTCGGCCTCCATCTCCTCCTCAACGCCGCCGCAACTCGAGAATTGCTCCAAGCTTCCTCCGCCTCCGGCTACAACGCGCCGGAACTGGTCAGGATGACTGACGCCGACGAACGGACCGACGTCTACAGCTACGGCAAGATCTTACTCGAGTTGCTCTCCGCCAAAGATACAATGAATCTAACTGCGGTTGCTTGTGGCGGAGACGGCGGCGATTGCAGTGTTTCGAAAGAGCGGAATCTCCAATGCCTTTTTCAGATCGGCGAGGCTTGTTGTTGTTCTTCGCCGATTCGGCGGCCGGATTTCAAGGAAGTTATTGAGAAGATTGAAGAAATCGGAAGGATTGAGATGGATTGA

Coding sequence (CDS)

ATGCTCAAATTAATCCTTATAGTCGCTTCCGCCACCATTTTTGTTCTTCTTCTAGTTTCCATCTTCTTTTTCCGCCATAAGCTCTCCAAAACAAACCACAGACACGACATCGAAAACCCATCGCCGGACAACAACCAGAAAAAACAAGACACGGCGGCAGCGACCGTGGAGGAGCTAACGACGTTTGAAGGCGGCGAAGATTTGACGATCGACGAGATTCTAGAAGCTCCGGGTGAAGTGATCGGAAAATACCACTATGGAACTCTGTACAAGGCTTCCTTACAGAGTACCCAGAGAACTACACTACTCATGTTCCTCCGCCCTGCCGCCGTGGAATCTAGTGTAGACGACGTCGTTCGGTTTCTGGGTTCGATCCGGCACCGGAATTTGGTGCCGATGTTGGGATTTTACGCCGGCGGGAGAGGCGAGAGACTTCTGGTTCATCCGTTTTATGGCAGAGGGAATCTGTCAGAGTTCATCACAGTGGGAGAATCGGAATCAAAAAATTGGGAAATCATCTACAAAATCTCTCTCGGAATCGCGAAAGCCCTAGATCATCTCCACCATGGATTACCAAAACCAGTACCGCACGGAAATCTCAAGCCCAAGAACATTTTTCTCGATCCAGATTTCGAGCCGTACGTCTCCGATTTCGGCCTCCATCTCCTCCTCAACGCCGCCGCAACTCGAGAATTGCTCCAAGCTTCCTCCGCCTCCGGCTACAACGCGCCGGAACTGGTCAGGATGACTGACGCCGACGAACGGACCGACGTCTACAGCTACGGCAAGATCTTACTCGAGTTGCTCTCCGCCAAAGATACAATGAATCTAACTGCGGTTGCTTGTGGCGGAGACGGCGGCGATTGCAGTGTTTCGAAAGAGCGGAATCTCCAATGCCTTTTTCAGATCGGCGAGGCTTGTTGTTGTTCTTCGCCGATTCGGCGGCCGGATTTCAAGGAAGTTATTGAGAAGATTGAAGAAATCGGAAGGATTGAGATGGATTGA

Protein sequence

MLKLILIVASATIFVLLLVSIFFFRHKLSKTNHRHDIENPSPDNNQKKQDTAAATVEELTTFEGGEDLTIDEILEAPGEVIGKYHYGTLYKASLQSTQRTTLLMFLRPAAVESSVDDVVRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGRGNLSEFITVGESESKNWEIIYKISLGIAKALDHLHHGLPKPVPHGNLKPKNIFLDPDFEPYVSDFGLHLLLNAAATRELLQASSASGYNAPELVRMTDADERTDVYSYGKILLELLSAKDTMNLTAVACGGDGGDCSVSKERNLQCLFQIGEACCCSSPIRRPDFKEVIEKIEEIGRIEMD
BLAST of Carg08499-RA vs. NCBI nr
Match: XP_022957896.1 (putative kinase-like protein TMKL1 [Cucurbita moschata])

HSP 1 Score: 655.6 bits (1690), Expect = 9.1e-185
Identity = 331/334 (99.10%), Postives = 332/334 (99.40%), Query Frame = 0

Query: 1   MLKLILIVASATIFVLLLVSIFFFRHKLSKTNHRHDIENPSPDNNQKKQDTAAATVEELT 60
           MLKLILIVASATIFVLLLVSIFF RHKLSKTNHR+DIENPSPDNNQKKQDTAAATVEELT
Sbjct: 1   MLKLILIVASATIFVLLLVSIFFLRHKLSKTNHRNDIENPSPDNNQKKQDTAAATVEELT 60

Query: 61  TFEGGEDLTIDEILEAPGEVIGKYHYGTLYKASLQSTQRTTLLMFLRPAAVESSVDDVVR 120
           TFEGGEDLTIDEILEAPGEVIGKYHYGTLYKASLQSTQRTTLLMFLRPAAVESSVDDVVR
Sbjct: 61  TFEGGEDLTIDEILEAPGEVIGKYHYGTLYKASLQSTQRTTLLMFLRPAAVESSVDDVVR 120

Query: 121 FLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGRGNLSEFITVGESESKNWEIIYKISLGI 180
           FLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGRGNLSEFITVGESESKNWEIIYKISLGI
Sbjct: 121 FLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGRGNLSEFITVGESESKNWEIIYKISLGI 180

Query: 181 AKALDHLHHGLPKPVPHGNLKPKNIFLDPDFEPYVSDFGLHLLLNAAATRELLQASSASG 240
           AKALDHLHHGLPKPVPHGNLKPKNIFLDPDFEPYVSDFGLHLLLNAAATRELLQASSASG
Sbjct: 181 AKALDHLHHGLPKPVPHGNLKPKNIFLDPDFEPYVSDFGLHLLLNAAATRELLQASSASG 240

Query: 241 YNAPELVRMTDADERTDVYSYGKILLELLSAKDTMNLTAVACGGDGGDCSVSKERNLQCL 300
           YNAPELVRMTDADERTDVYSYGKILLELLSAKDTMNLTAVACGGDGGDCSVSKERNLQCL
Sbjct: 241 YNAPELVRMTDADERTDVYSYGKILLELLSAKDTMNLTAVACGGDGGDCSVSKERNLQCL 300

Query: 301 FQIGEACCCSSPIRRPDFKEVIEKIEEIGRIEMD 335
           FQIGEACC SSPIRRPDFKEVIEKIEEIGRIEMD
Sbjct: 301 FQIGEACCSSSPIRRPDFKEVIEKIEEIGRIEMD 334

BLAST of Carg08499-RA vs. NCBI nr
Match: XP_023532398.1 (putative kinase-like protein TMKL1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 651.0 bits (1678), Expect = 2.2e-183
Identity = 329/334 (98.50%), Postives = 332/334 (99.40%), Query Frame = 0

Query: 1   MLKLILIVASATIFVLLLVSIFFFRHKLSKTNHRHDIENPSPDNNQKKQDTAAATVEELT 60
           MLKLILIVASATIFVLLLVSIFFFRHKLSKTNHR+DIENPSPDNNQKKQDTAAATVEELT
Sbjct: 1   MLKLILIVASATIFVLLLVSIFFFRHKLSKTNHRNDIENPSPDNNQKKQDTAAATVEELT 60

Query: 61  TFEGGEDLTIDEILEAPGEVIGKYHYGTLYKASLQSTQRTTLLMFLRPAAVESSVDDVVR 120
           TFEGGEDLTIDEILEAPGEVIGKYHYGTLYKASLQSTQRTTLLMFLRPAAVESSVDDVVR
Sbjct: 61  TFEGGEDLTIDEILEAPGEVIGKYHYGTLYKASLQSTQRTTLLMFLRPAAVESSVDDVVR 120

Query: 121 FLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGRGNLSEFITVGESESKNWEIIYKISLGI 180
           FLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGRGNLSEFITVGESESKNWEIIYKISLGI
Sbjct: 121 FLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGRGNLSEFITVGESESKNWEIIYKISLGI 180

Query: 181 AKALDHLHHGLPKPVPHGNLKPKNIFLDPDFEPYVSDFGLHLLLNAAATRELLQASSASG 240
           AKALDHLHHGLPK VPHGNLKPKNIFLDP+FEP+VSDFGLHLLLNAAATRELLQASSASG
Sbjct: 181 AKALDHLHHGLPKSVPHGNLKPKNIFLDPNFEPHVSDFGLHLLLNAAATRELLQASSASG 240

Query: 241 YNAPELVRMTDADERTDVYSYGKILLELLSAKDTMNLTAVACGGDGGDCSVSKERNLQCL 300
           YNAPELVRMTDADERTDVYSYGKILLELLSAKDTMNLTAVACGGDGGDCSVSKERNLQCL
Sbjct: 241 YNAPELVRMTDADERTDVYSYGKILLELLSAKDTMNLTAVACGGDGGDCSVSKERNLQCL 300

Query: 301 FQIGEACCCSSPIRRPDFKEVIEKIEEIGRIEMD 335
           FQIGEACC SSPIRRPDFKEVIEKIEEIGRIEMD
Sbjct: 301 FQIGEACCSSSPIRRPDFKEVIEKIEEIGRIEMD 334

BLAST of Carg08499-RA vs. NCBI nr
Match: XP_022994989.1 (putative kinase-like protein TMKL1 [Cucurbita maxima])

HSP 1 Score: 564.7 bits (1454), Expect = 2.1e-157
Identity = 280/299 (93.65%), Postives = 284/299 (94.98%), Query Frame = 0

Query: 36  DIENPSPDNNQKKQDTAAATVEELTTFEGGEDLTIDEILEAPGEVIGKYHYGTLYKASLQ 95
           DIENPS            ATVEELTTF+GGEDLTIDEILEAPGEVIGKYHYGTLYKASLQ
Sbjct: 36  DIENPSXXXXXXXXXXXXATVEELTTFQGGEDLTIDEILEAPGEVIGKYHYGTLYKASLQ 95

Query: 96  STQRTTLLMFLRPAAVESSVDDVVRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGRGN 155
           STQR TLLMFLRPAAVESSVDDVVRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGRGN
Sbjct: 96  STQRATLLMFLRPAAVESSVDDVVRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGRGN 155

Query: 156 LSEFITVGESESKNWEIIYKISLGIAKALDHLHHGLPKPVPHGNLKPKNIFLDPDFEPYV 215
           LSEFITVGESESKNWEIIYKISLGIAKALDHLHHGLPKPVPHGNLKP NIFLDP+FEPYV
Sbjct: 156 LSEFITVGESESKNWEIIYKISLGIAKALDHLHHGLPKPVPHGNLKPMNIFLDPNFEPYV 215

Query: 216 SDFGLHLLLNAAATRELLQASSASGYNAPELVRMTDADERTDVYSYGKILLELLSAKDTM 275
           SDFGLHLLLNAAATRELLQASSASGYNAPELV+MTDADERTDVYSYGKILLELLSAK+TM
Sbjct: 216 SDFGLHLLLNAAATRELLQASSASGYNAPELVKMTDADERTDVYSYGKILLELLSAKETM 275

Query: 276 NLTAVACGGDGGDCSVSKERNLQCLFQIGEACCCSSPIRRPDFKEVIEKIEEIGRIEMD 335
           N TAVACGGDGGDCSVSKERNLQCLFQIGEACCCSSPIRRPDFKEVIEKIEEIGRIEMD
Sbjct: 276 NQTAVACGGDGGDCSVSKERNLQCLFQIGEACCCSSPIRRPDFKEVIEKIEEIGRIEMD 334

BLAST of Carg08499-RA vs. NCBI nr
Match: XP_023520536.1 (putative kinase-like protein TMKL1 [Cucurbita pepo subsp. pepo] >XP_023520537.1 putative kinase-like protein TMKL1 [Cucurbita pepo subsp. pepo] >XP_023550283.1 putative kinase-like protein TMKL1 [Cucurbita pepo subsp. pepo] >XP_023550284.1 putative kinase-like protein TMKL1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 461.8 bits (1187), Expect = 1.9e-126
Identity = 234/308 (75.97%), Postives = 254/308 (82.47%), Query Frame = 0

Query: 27  KLSKTNHRHDIENPSPDNNQKKQDTAAATVEELTTFEGGEDLTIDEILEAPGEVIGKYHY 86
           KLS T HRHD+E P  D + KK+ T   +   L TFEGGEDLTIDEILEAPGEVIGK HY
Sbjct: 76  KLSLTEHRHDVERPCNDEHNKKEHTPPPSPPSLATFEGGEDLTIDEILEAPGEVIGKSHY 135

Query: 87  GTLYKASLQSTQRTTLLMFLRPAAVESSVDDVVRFLGSIRHRNLVPMLGFYAGGRGERLL 146
           GTLYKASLQSTQ T LL FLRP A      DVVRFLGSIRHRNLVPMLGFYAGGRGERLL
Sbjct: 136 GTLYKASLQSTQSTRLLRFLRPGAGGGEY-DVVRFLGSIRHRNLVPMLGFYAGGRGERLL 195

Query: 147 VHPFYGRGNLSEFITVGESESKNWEIIYKISLGIAKALDHLHHGLPKPVPHGNLKPKNIF 206
           VHPFYG+GNL+E I   ESESKNWE+I KISLGIA+ALDHLH+GL KP+PHGNLK KNI 
Sbjct: 196 VHPFYGQGNLAELIRERESESKNWEVINKISLGIARALDHLHNGLQKPIPHGNLKSKNIL 255

Query: 207 LDPDFEPYVSDFGLHLLLNAAATRELLQASSASGYNAPELVRMTDADERTDVYSYGKILL 266
           LD +FEP+VSDFGLHLLLNAAATRE+LQ S+ASGY APEL+ M+DADERTDV+SYGKILL
Sbjct: 256 LDSNFEPHVSDFGLHLLLNAAATREMLQVSAASGYKAPELINMSDADERTDVFSYGKILL 315

Query: 267 ELLSAKDTMNLTAVACGGDGGDCSVSKERNLQCLFQIGEACCCSSPIRRPDFKEVIEKIE 326
           ELLSAK+T        GGDGGDC VS+ER L+CL QIGEACC SSP  RPDFKEVI KI 
Sbjct: 316 ELLSAKET--------GGDGGDCGVSEERTLRCLLQIGEACCSSSPDLRPDFKEVIAKIT 374

Query: 327 EIGRIEMD 335
           EIGRIEMD
Sbjct: 376 EIGRIEMD 374

BLAST of Carg08499-RA vs. NCBI nr
Match: XP_022929086.1 (putative kinase-like protein TMKL1 [Cucurbita moschata] >XP_022929087.1 putative kinase-like protein TMKL1 [Cucurbita moschata] >XP_022929088.1 putative kinase-like protein TMKL1 [Cucurbita moschata])

HSP 1 Score: 460.3 bits (1183), Expect = 5.6e-126
Identity = 233/308 (75.65%), Postives = 254/308 (82.47%), Query Frame = 0

Query: 27  KLSKTNHRHDIENPSPDNNQKKQDTAAATVEELTTFEGGEDLTIDEILEAPGEVIGKYHY 86
           KLS T HRHD+E P  D + KK+ T   +   L TFEGGEDLTIDEILEAPGEVIGK HY
Sbjct: 29  KLSLTEHRHDVERPCNDEHNKKEHTPPPSPPSLATFEGGEDLTIDEILEAPGEVIGKSHY 88

Query: 87  GTLYKASLQSTQRTTLLMFLRPAAVESSVDDVVRFLGSIRHRNLVPMLGFYAGGRGERLL 146
           GTLYKASLQSTQ T LL FLRP A      DVVRFLGSIRHRNLVPMLGFYAGGRGERLL
Sbjct: 89  GTLYKASLQSTQATRLLRFLRPGAGGGEY-DVVRFLGSIRHRNLVPMLGFYAGGRGERLL 148

Query: 147 VHPFYGRGNLSEFITVGESESKNWEIIYKISLGIAKALDHLHHGLPKPVPHGNLKPKNIF 206
           VHPFYG+GNL+E I   ESESKNWE+I KISLGIA+ALDHLH+GL KP+PHGNLK KNI 
Sbjct: 149 VHPFYGQGNLAELIRERESESKNWEVINKISLGIARALDHLHNGLQKPIPHGNLKSKNIL 208

Query: 207 LDPDFEPYVSDFGLHLLLNAAATRELLQASSASGYNAPELVRMTDADERTDVYSYGKILL 266
           LD +FEP+VSDFGLHLLLNAAATRE+L+ S+ASGY APEL+ MTDADERTDV+SYGKILL
Sbjct: 209 LDSNFEPHVSDFGLHLLLNAAATREMLKVSAASGYKAPELINMTDADERTDVFSYGKILL 268

Query: 267 ELLSAKDTMNLTAVACGGDGGDCSVSKERNLQCLFQIGEACCCSSPIRRPDFKEVIEKIE 326
           ELLSA++T        GGDGGDC  S+ER L+CL QIGEACC SSP RRPDFKEVI KI 
Sbjct: 269 ELLSAEET--------GGDGGDCGGSEERTLRCLLQIGEACCSSSPDRRPDFKEVIAKIA 327

Query: 327 EIGRIEMD 335
           EIGRIEMD
Sbjct: 329 EIGRIEMD 327

BLAST of Carg08499-RA vs. TAIR10
Match: AT5G07620.1 (Protein kinase superfamily protein)

HSP 1 Score: 297.4 bits (760), Expect = 1.1e-80
Identity = 161/327 (49.24%), Postives = 215/327 (65.75%), Query Frame = 0

Query: 34  RHDIENPSPDNNQKKQDTAAATVEELTTFEGGEDLTIDEILEAPGEVIGKYHYGTLYKAS 93
           ++D+E  S D+N  KQ  ++ T EEL  F+GGEDLTI +IL+APGEVIGK  YGTLYKAS
Sbjct: 40  QYDVE--SLDHN--KQGFSSET-EELVIFQGGEDLTICDILDAPGEVIGKSSYGTLYKAS 99

Query: 94  LQSTQRTTLLMFLRPAAVESS----VDDVVRFLGSIRHRNLVPMLGFYAGGRGERLLVHP 153
           LQ + +  +L FLRP     S     + ++  LG +RH NLVP+LGFYAG RGE+L+VHP
Sbjct: 100 LQRSGKIRVLRFLRPVCTVRSDSKEFNGIIETLGFVRHENLVPLLGFYAGNRGEKLMVHP 159

Query: 154 FYGRGNLSEFITVGESESKNWEIIYKISLGIAKALDHLHHGLPKPVPHGNLKPKNIFLDP 213
           F+G GNLS+FI  G+ ES+ W  I +I++GI+KALDHLH G+ KP+ HGNLK KN+ L  
Sbjct: 160 FFGSGNLSDFIRSGDDESRKWINILRITIGISKALDHLHTGMQKPIVHGNLKSKNVLLSS 219

Query: 214 DFEPYVSDFGLHLLLNAAATRELLQASSASGYNAPELVRMTDADERTDVYSYGKILLELL 273
            FEP +SDFGLHLLLN +A +E+L  S+A GY APEL++M D  + +DVYS G I+LEL+
Sbjct: 220 SFEPRISDFGLHLLLNLSAGQEILDVSAAEGYKAPELIKMKDVSKESDVYSLGVIMLELV 279

Query: 274 SAKDTMNLTAVACGGD--------------------------GGDCSVSKERNLQCLFQI 331
           S K+ +N  A   G D                          G D ++S+E  L+  FQ+
Sbjct: 280 SGKEPINENAT--GDDEFYLPDFMRNAVLDHRLSDLYRPEILGSDDNLSEECVLK-YFQL 339

BLAST of Carg08499-RA vs. TAIR10
Match: AT5G61570.1 (Protein kinase superfamily protein)

HSP 1 Score: 290.4 bits (742), Expect = 1.4e-78
Identity = 167/359 (46.52%), Postives = 225/359 (62.67%), Query Frame = 0

Query: 1   MLKLILIVASATIFVLLLVSIFFF---RHKLSKTNH-RHDIENPSPDNNQKKQDTAAATV 60
           ++KLIL V+  T F++++         R   ++TN  ++D+E  SP   Q+  D  + T 
Sbjct: 6   LVKLILAVSLPTTFLIMIAXXXXXXXCRRTTTETNEVQYDVE--SPYEKQEFSDNGSETE 65

Query: 61  EELTTFEGGEDLTIDEILEAPGEVIGKYHYGTLYKASLQSTQRTTLLMFLRPAAVESS-- 120
           EEL  F GGEDLTI +IL+APGEVIGK  YGTLYKA+LQ + +  +L FLRP    +S  
Sbjct: 66  EELIIFNGGEDLTICDILDAPGEVIGKSSYGTLYKATLQRSGKVRVLRFLRPLCAVNSDS 125

Query: 121 --VDDVVRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYG-RGNLSEFITV---GESESK 180
              + V+  LG +RH NLVP+LGFY G RGE+L++HPF+G  GNLS FI     G+ ++ 
Sbjct: 126 KEFNGVIESLGFVRHDNLVPLLGFYVGNRGEKLMIHPFFGSSGNLSAFIKFLAGGDVDAH 185

Query: 181 NWEIIYKISLGIAKALDHLHHGLPKPVPHGNLKPKNIFLDPDFEPYVSDFGLHLLLNAAA 240
            W  I  I++GIAKALDHLH G+ KP+ HGNLK KN+ LD  F P VSDFGLHLLLN AA
Sbjct: 186 KWSNILSITIGIAKALDHLHTGMQKPIVHGNLKSKNVLLDKSFRPRVSDFGLHLLLNLAA 245

Query: 241 TRELLQASSASGYNAPELVRMTDADERTDVYSYGKILLELLSAKDTMNLTAVA------- 300
            +E+L+AS+A GY APEL++M +  + +DVYS+G I+LEL+S K+  N            
Sbjct: 246 GQEVLEASAAEGYKAPELIKMKEVSKESDVYSFGVIMLELVSGKEPTNKNPTGSVLDRNR 305

Query: 301 ------------CGGDGGDCSVSKERNLQCLFQIGEACCCSSPIRRPDFKEVIEKIEEI 329
                       C  DG    V++E  L+  FQ+  +CC  SP  RP FK+V+ K+EEI
Sbjct: 306 LSDLYRPEIIRRCLKDGN--GVTEECVLE-YFQLAMSCCSPSPTLRPSFKQVLRKLEEI 359

BLAST of Carg08499-RA vs. TAIR10
Match: AT3G24660.1 (transmembrane kinase-like 1)

HSP 1 Score: 164.1 bits (414), Expect = 1.5e-40
Identity = 107/350 (30.57%), Postives = 177/350 (50.57%), Query Frame = 0

Query: 4   LILIVASATIFVLLLVSIFFFRHKLSKTNHRHDIENPSPDNNQKKQDTAAATVEELTTFE 63
           +I +++ A +   LL+     + + S      D+E    ++   +++       +L  F+
Sbjct: 305 VIGLMSGAVVVASLLIGYLQNKKRKSSIESEDDLEEGDEEDEIGEKEGGEG---KLVVFQ 364

Query: 64  GGEDLTIDEILEAPGEVIGKYHYGTLYKASLQSTQRTTLLMFLRPAAVE-SSVDDVVRFL 123
           GGE+LT+D++L A G+V+ K  YGT+YKA L       L +       + SS   V+R L
Sbjct: 365 GGENLTLDDVLNATGQVMEKTSYGTVYKAKLSDGGNIALRLLREGTCKDRSSCLPVIRQL 424

Query: 124 GSIRHRNLVPMLGFYAGGRGERLLVHPFYGRGNLSEFI--TVGESESKNWEIIYKISLGI 183
           G IRH NLVP+  FY G RGE+LL++ +    +L + +  +     + NW   +KI+LGI
Sbjct: 425 GRIRHENLVPLRAFYQGKRGEKLLIYDYLPNISLHDLLHESKPRKPALNWARRHKIALGI 484

Query: 184 AKALDHLHHGLPKPVPHGNLKPKNIFLDPDFEPYVSDFGLHLLLNAAATRELLQASSASG 243
           A+ L +LH G   P+ HGN++ KN+ +D  F   +++FGL  ++  A   E++  + + G
Sbjct: 485 ARGLAYLHTGQEVPIIHGNIRSKNVLVDDFFFARLTEFGLDKIMVQAVADEIVSQAKSDG 544

Query: 244 YNAPELVRMTDADERTDVYSYGKILLELLSAK-------------DTMNLTAVACGGDGG 303
           Y APEL +M   + R+DVY++G +LLE+L  K             D  +L   A   +  
Sbjct: 545 YKAPELHKMKKCNPRSDVYAFGILLLEILMGKKPGKSGRNGNEFVDLPSLVKAAVLEETT 604

Query: 304 ----DCSVSK------ERNLQCLFQIGEACCCSSPIRRPDFKEVIEKIEE 328
               D    K      E  L    ++   CC      RP  +EV++++EE
Sbjct: 605 MEVFDLEAMKGIRSPMEEGLVHALKLAMGCCAPVTTVRPSMEEVVKQLEE 651

BLAST of Carg08499-RA vs. TAIR10
Match: AT3G51740.1 (inflorescence meristem receptor-like kinase 2)

HSP 1 Score: 146.4 bits (368), Expect = 3.2e-35
Identity = 94/292 (32.19%), Postives = 146/292 (50.00%), Query Frame = 0

Query: 58  ELTTFEGGEDLTIDEILEAPGEVIGKYHYGTLYKASLQSTQRTTLLMFLRPAAVE--SSV 117
           +L  F+G    T D++L A  E++GK  YGT YKA+L+      +   LR    +     
Sbjct: 520 KLVHFDGPFVFTADDLLCATAEIMGKSTYGTAYKATLEDGNEVAVKR-LREKTTKGVKEF 579

Query: 118 DDVVRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGRGNLSEFITV-GESESKNWEIIY 177
           +  V  LG IRH+NL+ +  +Y G +GE+LLV  +  +G+LS F+   G      WE   
Sbjct: 580 EGEVTALGKIRHQNLLALRAYYLGPKGEKLLVFDYMSKGSLSAFLHARGPETLIPWETRM 639

Query: 178 KISLGIAKALDHLHHGLPKPVPHGNLKPKNIFLDPDFEPYVSDFGLHLLLNAAATRELLQ 237
           KI+ GI++ L HLH    + + H NL   NI LD     +++D+GL  L+ AAA   ++ 
Sbjct: 640 KIAKGISRGLAHLHSN--ENMIHENLTASNILLDEQTNAHIADYGLSRLMTAAAATNVIA 699

Query: 238 ASSASGYNAPELVRMTDADERTDVYSYGKILLELLSAKD-------------TMNLTAVA 297
            +   GY APE  ++ +A  +TDVYS G I+LELL+ K                ++    
Sbjct: 700 TAGTLGYRAPEFSKIKNASAKTDVYSLGIIILELLTGKSPGEPTNGMDLPQWVASIVKEE 759

Query: 298 CGGDGGDCSVSKER-----NLQCLFQIGEACCCSSPIRRPDFKEVIEKIEEI 329
              +  D  + +E       L    ++   C   SP  RP+  +V+E++EEI
Sbjct: 760 WTNEVFDLELMRETQSVGDELLNTLKLALHCVDPSPAARPEANQVVEQLEEI 808

BLAST of Carg08499-RA vs. TAIR10
Match: AT3G17840.1 (receptor-like kinase 902)

HSP 1 Score: 136.0 bits (341), Expect = 4.4e-32
Identity = 89/285 (31.23%), Postives = 138/285 (48.42%), Query Frame = 0

Query: 70  IDEILEAPGEVIGKYHYGTLYKASLQSTQRTTLLMFLRPAAVESSVDDVVRFLGSIRHRN 129
           ++++L A  EV+GK  +GT YKA L +     +         +    + +  +G++ H N
Sbjct: 360 LEDLLRASAEVLGKGTFGTAYKAVLDAVTLVAVKRLKDVTMADREFKEKIEVVGAMDHEN 419

Query: 130 LVPMLGFYAGGRGERLLVHPFYGRGNLSEFITVGESESK---NWEIIYKISLGIAKALDH 189
           LVP+  +Y  G  E+LLV+ F   G+LS  +   +   +   NWE+   I+LG A+ LD+
Sbjct: 420 LVPLRAYYYSG-DEKLLVYDFMPMGSLSALLHGNKGAGRPPLNWEVRSGIALGAARGLDY 479

Query: 190 LHHGLPKPVPHGNLKPKNIFLDPDFEPYVSDFGLHLLLNAAATRELLQASSASGYNAPEL 249
           LH   P    HGN+K  NI L    +  VSDFGL  L++A++T      + A+GY APE+
Sbjct: 480 LHSQDPLS-SHGNVKSSNILLTNSHDARVSDFGLAQLVSASST----TPNRATGYRAPEV 539

Query: 250 VRMTDADERTDVYSYGKILLELLSAKDTMNLTAVACGGD--------------------- 309
                  ++ DVYS+G +LLELL+ K   N      G D                     
Sbjct: 540 TDPRRVSQKADVYSFGVVLLELLTGKAPSNSVMNEEGMDLARWVHSVAREEWRNEVFDSE 599

Query: 310 --GGDCSVSKERNLQCLFQIGEACCCSSPIRRPDFKEVIEKIEEI 329
               +  VS E  +  + Q+G  C    P +RP   EV+ +I+E+
Sbjct: 600 LMSIETVVSVEEEMAEMLQLGIDCTEQHPDKRPVMVEVVRRIQEL 638

BLAST of Carg08499-RA vs. Swiss-Prot
Match: sp|P33543|TMKL1_ARATH (Putative kinase-like protein TMKL1 OS=Arabidopsis thaliana OX=3702 GN=TMKL1 PE=1 SV=1)

HSP 1 Score: 164.1 bits (414), Expect = 2.7e-39
Identity = 107/350 (30.57%), Postives = 177/350 (50.57%), Query Frame = 0

Query: 4   LILIVASATIFVLLLVSIFFFRHKLSKTNHRHDIENPSPDNNQKKQDTAAATVEELTTFE 63
           +I +++ A +   LL+     + + S      D+E    ++   +++       +L  F+
Sbjct: 305 VIGLMSGAVVVASLLIGYLQNKKRKSSIESEDDLEEGDEEDEIGEKEGGEG---KLVVFQ 364

Query: 64  GGEDLTIDEILEAPGEVIGKYHYGTLYKASLQSTQRTTLLMFLRPAAVE-SSVDDVVRFL 123
           GGE+LT+D++L A G+V+ K  YGT+YKA L       L +       + SS   V+R L
Sbjct: 365 GGENLTLDDVLNATGQVMEKTSYGTVYKAKLSDGGNIALRLLREGTCKDRSSCLPVIRQL 424

Query: 124 GSIRHRNLVPMLGFYAGGRGERLLVHPFYGRGNLSEFI--TVGESESKNWEIIYKISLGI 183
           G IRH NLVP+  FY G RGE+LL++ +    +L + +  +     + NW   +KI+LGI
Sbjct: 425 GRIRHENLVPLRAFYQGKRGEKLLIYDYLPNISLHDLLHESKPRKPALNWARRHKIALGI 484

Query: 184 AKALDHLHHGLPKPVPHGNLKPKNIFLDPDFEPYVSDFGLHLLLNAAATRELLQASSASG 243
           A+ L +LH G   P+ HGN++ KN+ +D  F   +++FGL  ++  A   E++  + + G
Sbjct: 485 ARGLAYLHTGQEVPIIHGNIRSKNVLVDDFFFARLTEFGLDKIMVQAVADEIVSQAKSDG 544

Query: 244 YNAPELVRMTDADERTDVYSYGKILLELLSAK-------------DTMNLTAVACGGDGG 303
           Y APEL +M   + R+DVY++G +LLE+L  K             D  +L   A   +  
Sbjct: 545 YKAPELHKMKKCNPRSDVYAFGILLLEILMGKKPGKSGRNGNEFVDLPSLVKAAVLEETT 604

Query: 304 ----DCSVSK------ERNLQCLFQIGEACCCSSPIRRPDFKEVIEKIEE 328
               D    K      E  L    ++   CC      RP  +EV++++EE
Sbjct: 605 MEVFDLEAMKGIRSPMEEGLVHALKLAMGCCAPVTTVRPSMEEVVKQLEE 651

BLAST of Carg08499-RA vs. Swiss-Prot
Match: sp|Q9SCT4|IMK2_ARATH (Probably inactive leucine-rich repeat receptor-like protein kinase IMK2 OS=Arabidopsis thaliana OX=3702 GN=IMK2 PE=1 SV=1)

HSP 1 Score: 146.4 bits (368), Expect = 5.8e-34
Identity = 94/292 (32.19%), Postives = 146/292 (50.00%), Query Frame = 0

Query: 58  ELTTFEGGEDLTIDEILEAPGEVIGKYHYGTLYKASLQSTQRTTLLMFLRPAAVE--SSV 117
           +L  F+G    T D++L A  E++GK  YGT YKA+L+      +   LR    +     
Sbjct: 520 KLVHFDGPFVFTADDLLCATAEIMGKSTYGTAYKATLEDGNEVAVKR-LREKTTKGVKEF 579

Query: 118 DDVVRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGRGNLSEFITV-GESESKNWEIIY 177
           +  V  LG IRH+NL+ +  +Y G +GE+LLV  +  +G+LS F+   G      WE   
Sbjct: 580 EGEVTALGKIRHQNLLALRAYYLGPKGEKLLVFDYMSKGSLSAFLHARGPETLIPWETRM 639

Query: 178 KISLGIAKALDHLHHGLPKPVPHGNLKPKNIFLDPDFEPYVSDFGLHLLLNAAATRELLQ 237
           KI+ GI++ L HLH    + + H NL   NI LD     +++D+GL  L+ AAA   ++ 
Sbjct: 640 KIAKGISRGLAHLHSN--ENMIHENLTASNILLDEQTNAHIADYGLSRLMTAAAATNVIA 699

Query: 238 ASSASGYNAPELVRMTDADERTDVYSYGKILLELLSAKD-------------TMNLTAVA 297
            +   GY APE  ++ +A  +TDVYS G I+LELL+ K                ++    
Sbjct: 700 TAGTLGYRAPEFSKIKNASAKTDVYSLGIIILELLTGKSPGEPTNGMDLPQWVASIVKEE 759

Query: 298 CGGDGGDCSVSKER-----NLQCLFQIGEACCCSSPIRRPDFKEVIEKIEEI 329
              +  D  + +E       L    ++   C   SP  RP+  +V+E++EEI
Sbjct: 760 WTNEVFDLELMRETQSVGDELLNTLKLALHCVDPSPAARPEANQVVEQLEEI 808

BLAST of Carg08499-RA vs. Swiss-Prot
Match: sp|C0LGP9|IMK3_ARATH (Probable leucine-rich repeat receptor-like protein kinase IMK3 OS=Arabidopsis thaliana OX=3702 GN=IMK3 PE=1 SV=1)

HSP 1 Score: 141.4 bits (355), Expect = 1.9e-32
Identity = 81/226 (35.84%), Postives = 126/226 (55.75%), Query Frame = 0

Query: 50  DTAAATVEELTTFEGGEDLTIDEILEAPGEVIGKYHYGTLYKASLQSTQRTTLLMFLRPA 109
           +    T  +L  F+G    T D++L A  E++GK  YGT+YKA+L+   +  +   LR  
Sbjct: 463 EAGGETGGKLVHFDGPMAFTADDLLCATAEIMGKSTYGTVYKATLEDGSQVAVKR-LREK 522

Query: 110 AVES--SVDDVVRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGRGNLSEFITV-GESE 169
             +S    ++ +  LG IRH NL+ +  +Y G +GE+L+V  +  RG+L+ F+   G   
Sbjct: 523 ITKSQKEFENEINVLGRIRHPNLLALRAYYLGPKGEKLVVFDYMSRGSLATFLHARGPDV 582

Query: 170 SKNWEIIYKISLGIAKALDHLHHGLPKPVPHGNLKPKNIFLDPDFEPYVSDFGLHLLLNA 229
             NW     +  G+A+ L +LH      + HGNL   N+ LD +    +SD+GL  L+ A
Sbjct: 583 HINWPTRMSLIKGMARGLFYLH--THANIIHGNLTSSNVLLDENITAKISDYGLSRLMTA 642

Query: 230 AATRELLQASSASGYNAPELVRMTDADERTDVYSYGKILLELLSAK 273
           AA   ++  + A GY APEL ++  A+ +TDVYS G I+LELL+ K
Sbjct: 643 AAGSSVIATAGALGYRAPELSKLKKANTKTDVYSLGVIILELLTGK 685

BLAST of Carg08499-RA vs. Swiss-Prot
Match: sp|Q9LVI6|RLK90_ARATH (Probable inactive receptor kinase RLK902 OS=Arabidopsis thaliana OX=3702 GN=RLK902 PE=1 SV=1)

HSP 1 Score: 136.0 bits (341), Expect = 7.9e-31
Identity = 89/285 (31.23%), Postives = 138/285 (48.42%), Query Frame = 0

Query: 70  IDEILEAPGEVIGKYHYGTLYKASLQSTQRTTLLMFLRPAAVESSVDDVVRFLGSIRHRN 129
           ++++L A  EV+GK  +GT YKA L +     +         +    + +  +G++ H N
Sbjct: 360 LEDLLRASAEVLGKGTFGTAYKAVLDAVTLVAVKRLKDVTMADREFKEKIEVVGAMDHEN 419

Query: 130 LVPMLGFYAGGRGERLLVHPFYGRGNLSEFITVGESESK---NWEIIYKISLGIAKALDH 189
           LVP+  +Y  G  E+LLV+ F   G+LS  +   +   +   NWE+   I+LG A+ LD+
Sbjct: 420 LVPLRAYYYSG-DEKLLVYDFMPMGSLSALLHGNKGAGRPPLNWEVRSGIALGAARGLDY 479

Query: 190 LHHGLPKPVPHGNLKPKNIFLDPDFEPYVSDFGLHLLLNAAATRELLQASSASGYNAPEL 249
           LH   P    HGN+K  NI L    +  VSDFGL  L++A++T      + A+GY APE+
Sbjct: 480 LHSQDPLS-SHGNVKSSNILLTNSHDARVSDFGLAQLVSASST----TPNRATGYRAPEV 539

Query: 250 VRMTDADERTDVYSYGKILLELLSAKDTMNLTAVACGGD--------------------- 309
                  ++ DVYS+G +LLELL+ K   N      G D                     
Sbjct: 540 TDPRRVSQKADVYSFGVVLLELLTGKAPSNSVMNEEGMDLARWVHSVAREEWRNEVFDSE 599

Query: 310 --GGDCSVSKERNLQCLFQIGEACCCSSPIRRPDFKEVIEKIEEI 329
               +  VS E  +  + Q+G  C    P +RP   EV+ +I+E+
Sbjct: 600 LMSIETVVSVEEEMAEMLQLGIDCTEQHPDKRPVMVEVVRRIQEL 638

BLAST of Carg08499-RA vs. Swiss-Prot
Match: sp|Q9S7I6|RPK2_ARATH (LRR receptor-like serine/threonine-protein kinase RPK2 OS=Arabidopsis thaliana OX=3702 GN=RPK2 PE=2 SV=1)

HSP 1 Score: 134.4 bits (337), Expect = 2.3e-30
Identity = 104/350 (29.71%), Postives = 160/350 (45.71%), Query Frame = 0

Query: 9    ASATIFVLL-LVSIFFFRHKLSKTNHRHDIENPSPDNNQKKQDTAAATVEELTTF-EGGE 68
            ASA + VL+ LV +FF+  K                    K    A T  E+T F + G 
Sbjct: 818  ASAIVSVLIALVILFFYTRKW-----------------HPKSKIMATTKREVTMFMDIGV 877

Query: 69   DLTIDEILEAPG-----EVIGKYHYGTLYKASLQSTQRTTL--LMFLRPAAVESSVDDVV 128
             +T D ++ A G      +IG   +G  YKA +       +  L   R   V+    + +
Sbjct: 878  PITFDNVVRATGNFNASNLIGNGGFGATYKAEISQDVVVAIKRLSIGRFQGVQQFHAE-I 937

Query: 129  RFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGRGNLSEFITVGESESKNWEIIYKISLG 188
            + LG +RH NLV ++G++A    E  LV+ +   GNL +FI   E  +++W +++KI+L 
Sbjct: 938  KTLGRLRHPNLVTLIGYHA-SETEMFLVYNYLPGGNLEKFIQ--ERSTRDWRVLHKIALD 997

Query: 189  IAKALDHLHHGLPKPVPHGNLKPKNIFLDPDFEPYVSDFGLHLLLNAAATRELLQASSAS 248
            IA+AL +LH      V H ++KP NI LD D   Y+SDFGL  LL  + T      +   
Sbjct: 998  IARALAYLHDQCVPRVLHRDVKPSNILLDDDCNAYLSDFGLARLLGTSETHATTGVAGTF 1057

Query: 249  GYNAPELVRMTDADERTDVYSYGKILLELLSAKDTMNLTAVACGG--------------- 308
            GY APE        ++ DVYSYG +LLELLS K  ++ + V+ G                
Sbjct: 1058 GYVAPEYAMTCRVSDKADVYSYGVVLLELLSDKKALDPSFVSYGNGFNIVQWACMLLRQG 1117

Query: 309  ------DGGDCSVSKERNLQCLFQIGEACCCSSPIRRPDFKEVIEKIEEI 329
                    G        +L  +  +   C   S   RP  K+V+ +++++
Sbjct: 1118 RAKEFFTAGLWDAGPHDDLVEVLHLAVVCTVDSLSTRPTMKQVVRRLKQL 1146

BLAST of Carg08499-RA vs. TrEMBL
Match: tr|A0A1S3AT53|A0A1S3AT53_CUCME (putative kinase-like protein TMKL1 OS=Cucumis melo OX=3656 GN=LOC103482568 PE=4 SV=1)

HSP 1 Score: 374.4 bits (960), Expect = 2.7e-100
Identity = 194/276 (70.29%), Postives = 220/276 (79.71%), Query Frame = 0

Query: 59  LTTFEGGEDLTIDEILEAPGEVIGKYHYGTLYKASLQSTQRTTLLMFLRPAAV-ESSVDD 118
           LTTFEGG+DLTIDEILEAPGEVIGK HYGTLYKA LQ+TQ   LL FLRPAA       D
Sbjct: 60  LTTFEGGQDLTIDEILEAPGEVIGKSHYGTLYKAVLQTTQTIRLLRFLRPAAAGGEDQHD 119

Query: 119 VVRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGRGNLSEFITVGESESKNWEIIYKIS 178
           +VRFLGS+RHRNLVP+LGFYAGGRGE+LLVHPFY  GNL+EFI   + ESKNWE+I KI+
Sbjct: 120 LVRFLGSVRHRNLVPLLGFYAGGRGEKLLVHPFYEHGNLAEFIR-DDGESKNWEVICKIA 179

Query: 179 LGIAKALDHLHHGLPKPVPHGNLKPKNIFLDPDFEPYVSDFGLHLLLNAAATRELLQASS 238
           +GIA+AL HLH GL KP+ HGNL  KN+ LD + +P+VSDFGLHLLLN+AATRE+LQ S+
Sbjct: 180 IGIARALHHLHSGLQKPISHGNLNSKNVLLDQNLDPHVSDFGLHLLLNSAATREMLQVSA 239

Query: 239 ASGYNAPELVRMTDADERTDVYSYGKILLELL-SAKDTMNLTAVACGGDGGDCSVSKERN 298
           +SGY APEL+ MT ADERTDVYSYGKILLELL SAK+      VA     GD  VS E+ 
Sbjct: 240 SSGYKAPELINMTHADERTDVYSYGKILLELLISAKEPTIRNPVAT----GDSGVSGEQI 299

Query: 299 LQCLFQIGEACCCSSPIRRPDFKEVIEKIEEIGRIE 333
           L+CL QIGE CC SSP  RPDF+EVI KIEEI RIE
Sbjct: 300 LRCLVQIGEDCCRSSPNLRPDFREVIAKIEEIRRIE 330

BLAST of Carg08499-RA vs. TrEMBL
Match: tr|A0A0A0KKU6|A0A0A0KKU6_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G167070 PE=4 SV=1)

HSP 1 Score: 370.5 bits (950), Expect = 3.8e-99
Identity = 190/275 (69.09%), Postives = 217/275 (78.91%), Query Frame = 0

Query: 61  TFEGGEDLTIDEILEAPGEVIGKYHYGTLYKASLQSTQRTTLLMFLRPAAVE-SSVDDVV 120
           TFEGGEDLTIDEILEAPGEVIGK HYGTLYKA LQ+TQ   LL FLRPAA       D+V
Sbjct: 61  TFEGGEDLTIDEILEAPGEVIGKSHYGTLYKAVLQTTQTIRLLRFLRPAAAGCDDQHDLV 120

Query: 121 RFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGRGNLSEFITVGESESKNWEIIYKISLG 180
           R LGS+RH NLVP+LGFYAGGRGE+LLVHPFY  GNL+EFIT  + ESKN  +I KI++G
Sbjct: 121 RLLGSVRHPNLVPLLGFYAGGRGEKLLVHPFYEHGNLAEFIT--DKESKNGVVICKIAIG 180

Query: 181 IAKALDHLHHGLPKPVPHGNLKPKNIFLDPDFEPYVSDFGLHLLLNAAATRELLQASSAS 240
           IA+AL HLH+GL KP+PHGNL  KN+ LD +F P+VSDFGLHLLLN+ ATR++LQ S++S
Sbjct: 181 IARALHHLHNGLQKPIPHGNLNSKNVLLDQNFHPHVSDFGLHLLLNSPATRQMLQLSASS 240

Query: 241 GYNAPELVRMTDADERTDVYSYGKILLELLSAKDTMNLTAVACGGDGGDCSVSKERNLQC 300
           GY APEL+ MTDADE TDVYSYGKILLELL +     +   A  GD G   VS+E  LQC
Sbjct: 241 GYKAPELINMTDADELTDVYSYGKILLELLMSSKDPTVRNPAATGDSG---VSQEPILQC 300

Query: 301 LFQIGEACCCSSPIRRPDFKEVIEKIEEIGRIEMD 335
           L QIGE CC SSP  RPDF+EVI KIEEIGRIEM+
Sbjct: 301 LVQIGEDCCRSSPNLRPDFREVIAKIEEIGRIEMN 330

BLAST of Carg08499-RA vs. TrEMBL
Match: tr|A0A251KPE9|A0A251KPE9_MANES (Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_06G131900 PE=4 SV=1)

HSP 1 Score: 316.2 bits (809), Expect = 8.6e-83
Identity = 177/361 (49.03%), Postives = 234/361 (64.82%), Query Frame = 0

Query: 1   MLKLILIVASATIFVLLLVSIFFFRHKLSKTNHRHDIENPSPDNNQKKQDTAAATVEELT 60
           +LKLIL + SATIF+ L+V IF +  K    N   D+E+P   +  +K+      +E+L 
Sbjct: 6   LLKLILGLTSATIFISLMVVIFLY-FKNRARNDSIDVESPELKHGDEKE------MEDLL 65

Query: 61  TFEGGEDLTIDEILEAPGEVIGKYHYGTLYKASLQSTQRTTLLMFLRPAAVESSVD--DV 120
           TF+GG+DLTI +IL+APGEVIGK +YGTLYKA L  +    LL FLRP  +  + D  + 
Sbjct: 66  TFQGGQDLTISDILDAPGEVIGKSNYGTLYKALLHRSNCVRLLRFLRPVCIARAKDFEEA 125

Query: 121 VRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGRGNLSEFITVGESESKNWEIIYKISL 180
           ++ LGSIRH NLVP++GFYAG RGE+LLVHPF+ RG++++FI  G ++S  W IIY+ISL
Sbjct: 126 IQLLGSIRHPNLVPLVGFYAGPRGEKLLVHPFFRRGSMAQFIRDGNADSHKWTIIYRISL 185

Query: 181 GIAKALDHLHHGLPKPVPHGNLKPKNIFLDPDFEPYVSDFGLHLLLNAAATRELLQASSA 240
           GIAK LDHLH GL KPV HGNLK KNI LD +++PY+SDFGLHLLLN  A +E+L+AS+A
Sbjct: 186 GIAKGLDHLHTGLQKPVIHGNLKSKNILLDRNYQPYISDFGLHLLLNPTAGQEMLEASAA 245

Query: 241 SGYNAPELVRMTDADERTDVYSYGKILLELLSAK-------------------------- 300
            GY APEL++M DA E TD+YS G ILLELLS K                          
Sbjct: 246 DGYKAPELIKMKDASELTDIYSLGIILLELLSGKEPISENPTPNDDFHLPNFMRNAVLDR 305

Query: 301 ---DTMNLTAVACGGDGGDCSVSKERNLQCLFQIGEACCCSSPIRRPDFKEVIEKIEEIG 331
              D  +   +    +  +  V++E  L+  FQ+  ACC  SP  RP  ++V+ K+EEIG
Sbjct: 306 RITDLYHPDILLSNHNDNEGPVTEECVLK-FFQLAMACCSPSPSLRPTIRQVLWKLEEIG 358

BLAST of Carg08499-RA vs. TrEMBL
Match: tr|A0A2I4FHV5|A0A2I4FHV5_9ROSI (putative kinase-like protein TMKL1 OS=Juglans regia OX=51240 GN=LOC108998914 PE=4 SV=1)

HSP 1 Score: 316.2 bits (809), Expect = 8.6e-83
Identity = 167/329 (50.76%), Postives = 211/329 (64.13%), Query Frame = 0

Query: 32  NHRHDIENPSPDNNQKKQDTAAATVEELTTFEGGEDLTIDEILEAPGEVIGKYHYGTLYK 91
           N R DIE     N  +KQ   A T E+L  F GG+DLTI +IL+APGEVIGK ++GTLYK
Sbjct: 36  NGREDIE-----NMDQKQGVEAQT-EDLIKFHGGQDLTISDILDAPGEVIGKSNFGTLYK 95

Query: 92  ASLQSTQRTTLLMFLRP--AAVESSVDDVVRFLGSIRHRNLVPMLGFYAGGRGERLLVHP 151
           A +Q      LL FLRP  +A     DDV++ LG +RH NLVP+LGFYAG RGE+LLVHP
Sbjct: 96  ALMQQNSTLRLLRFLRPVCSARGMEFDDVIQLLGCVRHPNLVPLLGFYAGTRGEKLLVHP 155

Query: 152 FYGRGNLSEFITVGESESKNWEIIYKISLGIAKALDHLHHGLPKPVPHGNLKPKNIFLDP 211
           FY RGNL+EFI  G  ES+ W IIY IS+GI+K L+HLH G  KP+ HGNLK KN+ LD 
Sbjct: 156 FYRRGNLAEFIRDGNGESQTWAIIYSISMGISKGLEHLHTGFQKPIIHGNLKSKNVLLDR 215

Query: 212 DFEPYVSDFGLHLLLNAAATRELLQASSASGYNAPELVRMTDADERTDVYSYGKILLELL 271
           ++ PY+SDFGLHLLLNA A++E+L+AS+A GY APEL++M DA E TD+YS G ILLEL+
Sbjct: 216 NYHPYISDFGLHLLLNATASQEMLEASAAEGYKAPELIKMKDASEETDIYSLGVILLELI 275

Query: 272 SAKDTMN----------------------------LTAVACGGDGGDCSVSKERNLQCLF 331
           S ++ +N                               +    D GD S   E  +  LF
Sbjct: 276 SGREPINENPTPDEDFYLPNFMRNAVLGHRIGDLFRPEILLSSDNGDRSRVTEERILKLF 335

BLAST of Carg08499-RA vs. TrEMBL
Match: tr|U5GNN6|U5GNN6_POPTR (Uncharacterized protein OS=Populus trichocarpa OX=3694 GN=POPTR_004G232800v3 PE=4 SV=1)

HSP 1 Score: 313.5 bits (802), Expect = 5.6e-82
Identity = 181/360 (50.28%), Postives = 225/360 (62.50%), Query Frame = 0

Query: 2   LKLIL-IVASATIFVLLLVSIFFFRHKLSKTNHRHDIENPSPDNNQKKQDTAAATVEELT 61
           LKL++ +  +  +FV       FF+ K  +    +DIEN      +K  D  A T E+L 
Sbjct: 8   LKLVVGLTLAIFLFVXXXXXFVFFKRKAERKT-CNDIEN----TEEKHGDHGAETTEDLV 67

Query: 62  TFEGGEDLTIDEILEAPGEVIGKYHYGTLYKASLQSTQRTTLLMFLRPAAVESSVD--DV 121
           TF+GG+DLTI +IL+APGEVIGK +YGTLYKA LQ +    LL FLRP       D  DV
Sbjct: 68  TFQGGQDLTISDILDAPGEVIGKSNYGTLYKALLQRSNCVRLLRFLRPICTARVEDFGDV 127

Query: 122 VRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGRGNLSEFITVGESESKNWEIIYKISL 181
           V+ LG IRH NLVP+LGFYAG RGE+LLVHPF  RGNLS+FI  G+SE   W +IYKIS+
Sbjct: 128 VQLLGCIRHPNLVPLLGFYAGPRGEKLLVHPFLRRGNLSDFIRDGKSEFHKWTVIYKISI 187

Query: 182 GIAKALDHLHHGLPKPVPHGNLKPKNIFLDPDFEPYVSDFGLHLLLNAAATRELLQASSA 241
           GIAK LDHLH GL KPV HGNLK KNI LD +F P +SDFGLHLLLN  A +E+L+AS+A
Sbjct: 188 GIAKGLDHLHAGLQKPVIHGNLKSKNILLDRNFHPCISDFGLHLLLNLTAGQEMLEASAA 247

Query: 242 SGYNAPELVRMTDADERTDVYSYGKILLELLSAKDTMNLTAVA----------------- 301
            GY APEL++M +    TD+YS G ILLELLS K+ +N    A                 
Sbjct: 248 EGYKAPELIKMKEVTLETDIYSLGIILLELLSGKEPINENPTADEDFYLPTFMRNAVLDR 307

Query: 302 -----------CGGDGGDCSVSKERNLQCLFQIGEACCCSSPIRRPDFKEVIEKIEEIGR 331
                        GD  +  V++E  L+  FQ+  ACC  SP  RP+ K+V+ K+EEIGR
Sbjct: 308 RIADLYHPDILSNGDDNERPVTEECVLK-YFQLAMACCSPSPSLRPNIKQVLWKLEEIGR 361

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022957896.19.1e-18599.10putative kinase-like protein TMKL1 [Cucurbita moschata][more]
XP_023532398.12.2e-18398.50putative kinase-like protein TMKL1 [Cucurbita pepo subsp. pepo][more]
XP_022994989.12.1e-15793.65putative kinase-like protein TMKL1 [Cucurbita maxima][more]
XP_023520536.11.9e-12675.97putative kinase-like protein TMKL1 [Cucurbita pepo subsp. pepo] >XP_023520537.1 ... [more]
XP_022929086.15.6e-12675.65putative kinase-like protein TMKL1 [Cucurbita moschata] >XP_022929087.1 putative... [more]
Match NameE-valueIdentityDescription
AT5G07620.11.1e-8049.24Protein kinase superfamily protein[more]
AT5G61570.11.4e-7846.52Protein kinase superfamily protein[more]
AT3G24660.11.5e-4030.57transmembrane kinase-like 1[more]
AT3G51740.13.2e-3532.19inflorescence meristem receptor-like kinase 2[more]
AT3G17840.14.4e-3231.23receptor-like kinase 902[more]
Match NameE-valueIdentityDescription
sp|P33543|TMKL1_ARATH2.7e-3930.57Putative kinase-like protein TMKL1 OS=Arabidopsis thaliana OX=3702 GN=TMKL1 PE=1... [more]
sp|Q9SCT4|IMK2_ARATH5.8e-3432.19Probably inactive leucine-rich repeat receptor-like protein kinase IMK2 OS=Arabi... [more]
sp|C0LGP9|IMK3_ARATH1.9e-3235.84Probable leucine-rich repeat receptor-like protein kinase IMK3 OS=Arabidopsis th... [more]
sp|Q9LVI6|RLK90_ARATH7.9e-3131.23Probable inactive receptor kinase RLK902 OS=Arabidopsis thaliana OX=3702 GN=RLK9... [more]
sp|Q9S7I6|RPK2_ARATH2.3e-3029.71LRR receptor-like serine/threonine-protein kinase RPK2 OS=Arabidopsis thaliana O... [more]
Match NameE-valueIdentityDescription
tr|A0A1S3AT53|A0A1S3AT53_CUCME2.7e-10070.29putative kinase-like protein TMKL1 OS=Cucumis melo OX=3656 GN=LOC103482568 PE=4 ... [more]
tr|A0A0A0KKU6|A0A0A0KKU6_CUCSA3.8e-9969.09Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G167070 PE=4 SV=1[more]
tr|A0A251KPE9|A0A251KPE9_MANES8.6e-8349.03Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_06G131900 PE=4 SV=... [more]
tr|A0A2I4FHV5|A0A2I4FHV5_9ROSI8.6e-8350.76putative kinase-like protein TMKL1 OS=Juglans regia OX=51240 GN=LOC108998914 PE=... [more]
tr|U5GNN6|U5GNN6_POPTR5.6e-8250.28Uncharacterized protein OS=Populus trichocarpa OX=3694 GN=POPTR_004G232800v3 PE=... [more]
The following terms have been associated with this mRNA:
Vocabulary: Molecular Function
TermDefinition
GO:0005524ATP binding
GO:0004672protein kinase activity
Vocabulary: Biological Process
TermDefinition
GO:0006468protein phosphorylation
Vocabulary: INTERPRO
TermDefinition
IPR011009Kinase-like_dom_sf
IPR000719Prot_kinase_dom
IPR001245Ser-Thr/Tyr_kinase_cat_dom
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0005575 cellular_component
molecular_function GO:0005524 ATP binding
molecular_function GO:0004672 protein kinase activity

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Carg08499Carg08499gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg08499-RA.exon.1Carg08499-RA.exon.1exon
Carg08499-RA.exon.2Carg08499-RA.exon.2exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg08499-RA.CDS.1Carg08499-RA.CDS.1CDS
Carg08499-RA.CDS.2Carg08499-RA.CDS.2CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Carg08499-RACarg08499-RA-proteinpolypeptide


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePIRSFPIRSF000654ILKcoord: 109..333
e-value: 6.1E-22
score: 75.8
NoneNo IPR availableGENE3DG3DSA:1.10.510.10coord: 68..334
e-value: 2.4E-45
score: 156.7
NoneNo IPR availablePANTHERPTHR27008:SF13PROTEIN KINASE FAMILY PROTEIN-RELATEDcoord: 8..278
NoneNo IPR availablePANTHERPTHR27008FAMILY NOT NAMEDcoord: 8..278
IPR001245Serine-threonine/tyrosine-protein kinase, catalytic domainPFAMPF07714Pkinase_Tyrcoord: 79..324
e-value: 4.2E-22
score: 78.6
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 75..328
score: 22.605
IPR011009Protein kinase-like domain superfamilySUPERFAMILYSSF56112Protein kinase-like (PK-like)coord: 78..326