Carg07425-RA (mRNA) Silver-seed gourd

NameCarg07425-RA
TypemRNA
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionMethionine aminopeptidase 2
LocationCucurbita_argyrosperma_scaffold_019 : 730789 .. 736862 (+)
Sequence length1457
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTTCATTTGACACTTACTTTAATCTCTCGCTCATAACCGAAGGGTCGGCCAGTTTCAGTCGCAAAACAGGGCAAATCTTGGTTGATACTCAATTTCTTCAAAGAACCCACAGACCTTCAAGTCTTCTCTTCTCAGTGTACTGTTAGTAAGTCTTGATCACAGGGTTCTTCCATTTACAATTTTCCTGTTCTTCTTAATGCAGCTTAGGTAGAATCATTCCATAAGTTCCTCACTCGTTTCCCTCTGTTTTGTTTGATTTACATTACGCTTTACTTTTTTGTTCTTCTGTTTTGTTATAGGAAGCGCCGCCGAGGAGGGTTTTTCAATCATGGCTGATGAAAATCACACTGGAATTCGTGTCCCTGAAGATGGGGCCTCAGAACCCAGTAATGGGAATGAACCAGATTCTGTTGAACTTGCTTCCACATCGCAAAAGGATGAAGATGATTTGAAAGGTGAATTGTTTTGCCTTGTGAAAGATGATCGTGGGTCAAGGTTTCTTATGCTTATGAATAGTTAGAAATTGTTAAAGGAAAATTAAAGTTTTCACATCCTTTGGTTGTGTATACTAATGGGGCTTGATGGGAAGATAAAATATAGGTCGTTGTATCTGTTTTTCTTAAATAGATTTAGAATGATTGTTATTGTAGTTTATTAATATTCTGAGTGTAGAGTTTAGAGATCATGGTTGGGGAGGAGAACGAAACATCCGTTATAAGGGTGTGGAGACCTCTCCCTAGCAGACATGTTTTACAAACCTTGAGGGAAAGCTCGAAAGGGAAAGCTCAAATAGGACAATATCTGCTAGAAGTGAGCTTGGGCCATTAGAAATGGTATCAGAGCCAGACATGGATGATGTGCCAGCGAGGAGGCTGAGCTTCGAAGGGGGGTGGACACGAGGCGGTGTGCAACAAGAACGCTGGGCCCTAAAGGGGGGTGAATTGAGGGCTCCCACATCGATTGGAGGGAGGAATGAGTGCTAGCGAGGACGTTGAGCCTCGAAGGAGGGTGGATTGTGAGATTCCACATCGGTTGGGGAGGAGAACGAAACATCATTTATAAGGGTGTGGAAACCTCTCCCTAGTAGATGCGTTTTAAAAACTTTGAGAGTTTACTGATGCTTGTATTTCTCATCGCAACGTAAACCGTAACTTTGTACCCTGTCAAATGATTATTTAATTTGATATAACTGGTGGTTGTATCTACTTCCAGTTTATTGATGGTTTACTGATGCTTGCAATTGGCTGATATTTATCCTTCATTTTGCAGAAGGAACAAAGAAAAAGAAGAAGAAAAATAAAAGCAAGTGAGAACTCTTTATTTTTAGTACATTGTTTCTTGTTTGTGTATGCTTTCCCCTAGGTTGGGGTGTTGATGGATATTGCAAGGCTATCTTTAAGTTTGATATATATTATTTTTTTTACATGAATAGGAAAAAGAAAGAGACACCTCAACAGACGGATCCACCGTCTATTCCCGTCGTTGATCTTTTCCCGTCCGGGGATTTTCCAGAGGGTGAAATTCAACAGTACAAGGATGAGTGAGTAAAAGTCTTGATTTTAATGCTGCATTGTAATAGTTTTCTTGTCTAATATTATGGTTCAAAACATATGTTGTTTTGGATTGAATTGAGAAATCAAGAATAATATATTAAACACGGTATATGTAACAACACAAGCCCACCGCTAGCAGATATTATCTTCTTTGGGCTTTCCCTTTCGGGCTTCCCCTCAAGGTTAATAAAACGCTTCTGCTCGAGAGATGTTTTCACGCCCTTATAAAGAATATTTCTTTCTCCTCCCCAACCGATGTGGGATCTCACAGTCCACCCCTCTTCGGGGCCCAGCGTCCTCGCTGGCACTCGTTCCCTTCTCCAATCAATGTGAGACCATTAATCCACCCCCTATGGGGCCAGCGTCCTTGTTGTTACACCACCTCATGTCTACCCCCGTCGGGGCTTAGCCTCCTCGCTGGCACATCGCCCGGTGTCTGACTTTGATACCATTTGTAACGCCTCAAGCCCACCGCTAGCAGATATTGTTTTCTTTGGACTTTTCCTTTCGAGTTTTCCCTCAAGGTTTTTAGAACACGTCTGCTAGGGAGAGGTTTACAGTATACACTTGTAACTTCCACTTGTAACTTCCACTTGTAACTTGTAAGTTGTCTTTCAAAGTAAGTGGAAAAACACAGTAAGGTACTGGAAAGGAGCTTTACATATGTACTTGTTGTTGTGTAATTTTTGCATTACGAGTCAAGTTTTAATATTGTGATTTGAGAACATAGTATCGTCCACATGGTTGGTCTTTTAAACTCACTTAATTAACCTGAGTACTATCACCCAATTTTATTTAGATAATGAAATCGAATGATGATTGAAAAAATAAGTAAAGCAATGATTAAAAATTAAGATAGAAACAGTCTCGAGAGAATATCTTACGGAGTTGATCTCGCCTATTAGGCCAATAAACTACTTAAATATCCAATATTGATCATGCAAATGTTCCATAAGTCTAGAAGTCTATATCATACATGGCTCTTTGTTCTCTGTTTAGTAACCTATGGAGGACTACATCTGAAGAGAAGAGGGAGTTGGAGCGCCTTGAAAAGCCAATCTATAATTCTGTTCGCCGAGCAGCTGAAGTTCATCGTCAGGTAATGTAACTTGATTATATTCATGGTTATGTTGCCATATCGTGTGGTTTAATGAGACTGCTGTTAGTATGAATTATCTAACGTTTTTTTTCTGCTCATTGCTATTAGGTAAGAAGATACATAAAAAGCATACTAAAGCCAGGGATGTTGATGACTGACCTGTGTGAGACATTAGAGAACACAGTTAGGAAGCTCATATCTGAGAATGGCTTGCAAGCAGGCATAGCATTTCCTACAGGATGCTCTTTGAACTGGTAAAATATTAAAGAAGTGGCTTCAAATATGTGTGTGTGTATAGTTCTTCAGTATTTGTTGATAGATTAGGCTTCTATTCTTTCAGGGTTGCTGCTCATTGGACTCCTAATACTGGAGATAAGACTGTTCTTAACTACGACGATGTAATGAAATTGGATTTTGGAACGCATATTGATGGTATATATATACTTTTTGTTTCAGTTTTTTTGTATTATATGGATCGTAAAAGCCTAAGCCCACCGCTAGCAGATATTGTCCGTTTGGCCCGTTGAGGGGAAGCTTGAAAGGGAAAAATCAAACCCACCGCTGGCAGATATTGTCTACTTTGGCTCGTCGAGGGGAAGCTCGAAAGGGAAAGTCCAAAGAGGACAATATTTGCTAGTGGTAGGCTTGGGTTGTTACAAAAGACGCGTTTTAAAATCTTAAGCGGAAGTCTAGAAGAGAAAGCCCAAAAAGGACAATATCTGCTAGCGGTGGGCTTGGCTTGGGCTGTTACAATATCATATAACTGAAACTGGTTTATTGGTGAGTGCAGGATGCATAGTTGATTGTGCATTTACTGTTGCATTCAATCCTATGTTTGATCCTCTGCTTGAAGCCTCGCGTGAGGCAACTAACACTGGCATCAAGGTATTGTTAATTATCCTGTCTGCTGGCGGTCACTATAACATTTTGATTACTCTGCCTTGATGAAGTATATTATACTGTTTCATCTTATGAAATTACAAGTATCTGCGGTGGTTGAACAAATTGTGAGAAAATGCTGAGTTCTAGTGTGAGATCCCACGTCGGTTGGAGAGGAGAACGAAGCAATCTTTATAAGAGTGTGGAAACCTCTTCCTAGTACATGCGTTTTTAAAACCTTGAGGGGACCCCAAAGAGGACAATATCTACTAGCAGTGGGCTTGGACTGTTATAAATGTTATCAAAACCAGACATTGGGCGATGTACTGGCGAGGAGGCTAAGCCTCAATGAGGGGTGGAGACGAAGTGATGTGCCAGCAAGGACGCTGGGCCCCAAAGTTGGTGGATTGGAGGGTTCCACATCGATTGGAGAAGAGAACAAGTGCCAGCGAGGACCCTGGGCCCCGAAGGAGGTGAATTGTGAGATTCCACGTCGGTTGGGAGGAGAATGAAACAATCTTTATAAGGGTGTGGAAACCTCTCCTTTGCAGACGCGTTTTAAAAATTTTGAGGAAAAGCCCGAAAGGGAAAACTCAAATAGTACAATATCTGTTAGCGGTGGGTTTGAGTTTGGACGGTCACACATGTATATATCAAGTAAATAACAAAGAATATCAATCCATTTGTGAAAAGGTTCGATAGAATATGTTTATTCCATGTAATCAATAATTTGTTTGTTCTCTTAAATTTTGCACTACAAATCACTAGAATTTTATTTATATATATTCCGAATGAATTTGATTGTTTCTTGAACCTCTACAATTAACTATCTTTTATTTCTTTAGGAGTCTGGAATCGATGTACGACTTTGTGACGTTGGTGCTGCAATCCAGGAGGTCATGGAGTCGTATGAGGTTGAAATTAACGGAAAGGTGTTTCAAGGTACGAGTATTTCATTTGTCATCAAAGTAGAAGTCATTTGTTATCTTTTGAAAACTAGGCTATACTCAGAGTTCTCATGTGAGATCTCACATCGGTTGGAGAGGGGAATGAAACATTTCTTATAAAGGTATGGAAACCTCTCCCTACCCGATGCGTTTTAAAACCTTGAAGGGAAGCTTAGAAGGGAAAGCCCAAAAAGGACAATATCAGCTAGCGGTGGGCTTGAGTTGTTACAAATGGTATTAGAGCTTGACACTGGATTTTGTGCTAGCAAGGACGCTAGGCCTCCAAGAGGGGTGGACTATGAGATCTCACGTCGGTTGGAGTGGGGAACGAAGCATTCCTTATAAGGGTGTGGAAACCTCTCTCTACCATATGCGTTTTAAAACCTTGAGGGGAATCCAGAAGAGAAAGCCCAAAGAGGACAATATCTATTAGCGGTAGGCTTGAGCTATTACATCTTAACTCACCTATTCTTTATTATATTGACGCAAACAATGTTGTTACCCTCCCCGAAGATCATATTTCATGAAAATTAGATTTTAATAAACGATATTTTTCTCTTTTCAGTTAAAAGCATTCGAAATTTGAATGGACATAGCATTGGTCGTTACCAGATCCATGCTGGTAAATCTGTTCCCATTGTGAAAGGAGGGGAGCAAACCAAAATGGAAGAGGGTGAATTCTTCGCTATCGAAACGTTTGGATCGACGGGTAAGAAAGTACGCAGCTTGGAAGTTATTGCTATTTATTACGGTTTGCCCCTGTTGATAGGGCAGTTCATCTGTGTTTTATCCAGGGAAAGGCTATGTTCGAGAAGATCTTGAATGTAGCCATTACATGAAGAACTTTGACGTTGGCCATATACCTCTGAGGTTGCCAAGAGCTAAGCAATTATTGGCCACCATTAATAAGAACTTCTCAACATTGGCATTCTGCAAACGATATCTCGATCGCCTGGGCGAGACGAAATATCTAATGGGATTGAAGAATTTATGTGATTCCGGGATCGTTCAGGTGCTCCTCTTATACCTATTGTCTCGATATCTTCGATATATTATTAAGAGATGAATTCATATGACTTAGCTCGGAGTTTCTCTGGTTTCCCTTCTGTTAGTGCACCATGTTATATTTAGCACGTTAGTTCGTTGTGACTTCGATATCGATTTTGATTGCTGCTGGTGTGTTTGGGAGTGCTTTTAGCAAGAACCGTGTTTATATATATGTACATCCGTAGCAACGTGTCAGTCGATAATTCGGTTCTTTTCTCAGGCCTATCCTCCGTTGTGCGACTCGAAGGGCAGCTATGTTTCTCAGTTTGAGCATACCATTTTACTAAGGCCAACCTGCAAAGAAGTGATTTCCAGAGGTGATGACTATTGATATACTACTGTGGTGTGGCAGTTCCAAAGCCTTTCTCATTGAGACATGGTTACTTATGAGCATTGGTTTAAGTTACATCTTTTTGTACGATATTTGGTTAGTTCAAGAAGATCAAATACAACAAATTGTTATATTTTTATATAGTTCTTATGTTCTTTTGATACTTCAAATTACTCGAGCAACTCTAAAATCAGTAAAAAGATCAAAATTTTAAATT

mRNA sequence

TTTTCATTTGACACTTACTTTAATCTCTCGCTCATAACCGAAGGGTCGGCCAGTTTCAGTCGCAAAACAGGGCAAATCTTGGTTGATACTCAATTTCTTCAAAGAACCCACAGACCTTCAAGTCTTCTCTTCTCAGTGTACTGTTAGAAGCGCCGCCGAGGAGGGTTTTTCAATCATGGCTGATGAAAATCACACTGGAATTCGTGTCCCTGAAGATGGGGCCTCAGAACCCAGTAATGGGAATGAACCAGATTCTGTTGAACTTGCTTCCACATCGCAAAAGGATGAAGATGATTTGAAAGAAGGAACAAAGAAAAAGAAGAAGAAAAATAAAAGCAAGAAAAAGAAAGAGACACCTCAACAGACGGATCCACCGTCTATTCCCGTCGTTGATCTTTTCCCGTCCGGGGATTTTCCAGAGGGTGAAATTCAACAGTACAAGGATGATAACCTATGGAGGACTACATCTGAAGAGAAGAGGGAGTTGGAGCGCCTTGAAAAGCCAATCTATAATTCTGTTCGCCGAGCAGCTGAAGTTCATCGTCAGGTAAGAAGATACATAAAAAGCATACTAAAGCCAGGGATGTTGATGACTGACCTGTGTGAGACATTAGAGAACACAGTTAGGAAGCTCATATCTGAGAATGGCTTGCAAGCAGGCATAGCATTTCCTACAGGATGCTCTTTGAACTGGGTTGCTGCTCATTGGACTCCTAATACTGGAGATAAGACTGAGTCTGGAATCGATGTACGACTTTGTGACGTTGTTAAAAGCATTCGAAATTTGAATGGACATAGCATTGGTCGTTACCAGATCCATGCTGGTAAATCTGTTCCCATTGTGAAAGGAGGGGAGCAAACCAAAATGGAAGAGGGTGAATTCTTCGCTATCGAAACGTTTGGATCGACGGGGAAAGGCTATGTTCGAGAAGATCTTGAATGTAGCCATTACATGAAGAACTTTGACGTTGGCCATATACCTCTGAGGTTGCCAAGAGCTAAGCAATTATTGGCCACCATTAATAAGAACTTCTCAACATTGGCATTCTGCAAACGATATCTCGATCGCCTGGGCGAGACGAAATATCTAATGGGATTGAAGAATTTATGTGATTCCGGGATCGTTCAGGCCTATCCTCCGTTGTGCGACTCGAAGGGCAGCTATGTTTCTCAGTTTGAGCATACCATTTTACTAAGGCCAACCTGCAAAGAAGTGATTTCCAGAGGTGATGACTATTGATATACTACTGTGGTGTGGCAGTTCCAAAGCCTTTCTCATTGAGACATGGTTACTTATGAGCATTGGTTTAAGTTACATCTTTTTGTACGATATTTGGTTAGTTCAAGAAGATCAAATACAACAAATTGTTATATTTTTATATAGTTCTTATGTTCTTTTGATACTTCAAATTACTCGAGCAACTCTAAAATCAGTAAAAAGATCAAAATTTTAAATT

Coding sequence (CDS)

ATGGCTGATGAAAATCACACTGGAATTCGTGTCCCTGAAGATGGGGCCTCAGAACCCAGTAATGGGAATGAACCAGATTCTGTTGAACTTGCTTCCACATCGCAAAAGGATGAAGATGATTTGAAAGAAGGAACAAAGAAAAAGAAGAAGAAAAATAAAAGCAAGAAAAAGAAAGAGACACCTCAACAGACGGATCCACCGTCTATTCCCGTCGTTGATCTTTTCCCGTCCGGGGATTTTCCAGAGGGTGAAATTCAACAGTACAAGGATGATAACCTATGGAGGACTACATCTGAAGAGAAGAGGGAGTTGGAGCGCCTTGAAAAGCCAATCTATAATTCTGTTCGCCGAGCAGCTGAAGTTCATCGTCAGGTAAGAAGATACATAAAAAGCATACTAAAGCCAGGGATGTTGATGACTGACCTGTGTGAGACATTAGAGAACACAGTTAGGAAGCTCATATCTGAGAATGGCTTGCAAGCAGGCATAGCATTTCCTACAGGATGCTCTTTGAACTGGGTTGCTGCTCATTGGACTCCTAATACTGGAGATAAGACTGAGTCTGGAATCGATGTACGACTTTGTGACGTTGTTAAAAGCATTCGAAATTTGAATGGACATAGCATTGGTCGTTACCAGATCCATGCTGGTAAATCTGTTCCCATTGTGAAAGGAGGGGAGCAAACCAAAATGGAAGAGGGTGAATTCTTCGCTATCGAAACGTTTGGATCGACGGGGAAAGGCTATGTTCGAGAAGATCTTGAATGTAGCCATTACATGAAGAACTTTGACGTTGGCCATATACCTCTGAGGTTGCCAAGAGCTAAGCAATTATTGGCCACCATTAATAAGAACTTCTCAACATTGGCATTCTGCAAACGATATCTCGATCGCCTGGGCGAGACGAAATATCTAATGGGATTGAAGAATTTATGTGATTCCGGGATCGTTCAGGCCTATCCTCCGTTGTGCGACTCGAAGGGCAGCTATGTTTCTCAGTTTGAGCATACCATTTTACTAAGGCCAACCTGCAAAGAAGTGATTTCCAGAGGTGATGACTATTGA

Protein sequence

MADENHTGIRVPEDGASEPSNGNEPDSVELASTSQKDEDDLKEGTKKKKKKNKSKKKKETPQQTDPPSIPVVDLFPSGDFPEGEIQQYKDDNLWRTTSEEKRELERLEKPIYNSVRRAAEVHRQVRRYIKSILKPGMLMTDLCETLENTVRKLISENGLQAGIAFPTGCSLNWVAAHWTPNTGDKTESGIDVRLCDVVKSIRNLNGHSIGRYQIHAGKSVPIVKGGEQTKMEEGEFFAIETFGSTGKGYVREDLECSHYMKNFDVGHIPLRLPRAKQLLATINKNFSTLAFCKRYLDRLGETKYLMGLKNLCDSGIVQAYPPLCDSKGSYVSQFEHTILLRPTCKEVISRGDDY
BLAST of Carg07425-RA vs. NCBI nr
Match: XP_022948436.1 (methionine aminopeptidase 2B isoform X1 [Cucurbita moschata])

HSP 1 Score: 619.0 bits (1595), Expect = 1.0e-173
Identity = 353/424 (83.25%), Postives = 353/424 (83.25%), Query Frame = 0

Query: 1   MADENHTGIRVPEDGASEPSNGNEPDSVELASTSQKXXXXXXXXXXXXXXXXXXXXXXXX 60
           MADENHTGIRVPEDGASEPSNGNEPDSVELASTSQKXXXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 1   MADENHTGIRVPEDGASEPSNGNEPDSVELASTSQKXXXXXXXXXXXXXXXXXXXXXXXX 60

Query: 61  XXXXXXPSIPVVDLFPSGDFPEGEIQQYKDDNLWRTTSEEKRELERLEKPIYNSVRRAAE 120
           XXXXXXPSIPVVDLFPSGDFPEGEIQQYKDDNLWRTTSEE RELERLEKPIYNSVRRAAE
Sbjct: 61  XXXXXXPSIPVVDLFPSGDFPEGEIQQYKDDNLWRTTSEEMRELERLEKPIYNSVRRAAE 120

Query: 121 VHRQVRRYIKSILKPGMLMTDLCETLENTVRKLISENGLQAGIAFPTGCSLNWVAAHWTP 180
           VHRQVRRYIKSILKPGMLMTDLCETLENTVRKLISENGLQAGIAFPTGCSLNWVAAHWTP
Sbjct: 121 VHRQVRRYIKSILKPGMLMTDLCETLENTVRKLISENGLQAGIAFPTGCSLNWVAAHWTP 180

Query: 181 NTGDKT-------------------------------------------------ESGID 240
           NTGDKT                                                 ESGID
Sbjct: 181 NTGDKTVLNYDDVMKLDFGTHIDGCIVDXXXXXXXXXXXXXXXXXXXXXXNTGIKESGID 240

Query: 241 VRLCDV---------------------VKSIRNLNGHSIGRYQIHAGKSVPIVKGGEQTK 300
           VRLCDV                     VKSIRNLNGHSIGRYQIHAGKSVPIVKGGEQTK
Sbjct: 241 VRLCDVGAAIQEVMESYEVEINGKVFQVKSIRNLNGHSIGRYQIHAGKSVPIVKGGEQTK 300

Query: 301 MEEGEFFAIETFGSTGKGYVREDLECSHYMKNFDVGHIPLRLPRAKQLLATINKNFSTLA 355
           MEEGEFFAIETFGSTGKGYVREDLECSHYMKNFDVGHIPLRLPRAKQLLATINKNFSTLA
Sbjct: 301 MEEGEFFAIETFGSTGKGYVREDLECSHYMKNFDVGHIPLRLPRAKQLLATINKNFSTLA 360

BLAST of Carg07425-RA vs. NCBI nr
Match: XP_023521739.1 (methionine aminopeptidase 2B isoform X1 [Cucurbita pepo subsp. pepo] >XP_023521748.1 methionine aminopeptidase 2B isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 611.7 bits (1576), Expect = 1.6e-171
Identity = 349/424 (82.31%), Postives = 351/424 (82.78%), Query Frame = 0

Query: 1   MADENHTGIRVPEDGASEPSNGNEPDSVELASTSQKXXXXXXXXXXXXXXXXXXXXXXXX 60
           MADENHT IRVPEDGASEPSNGNEPDSVELASTSQ XXXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 1   MADENHTEIRVPEDGASEPSNGNEPDSVELASTSQXXXXXXXXXXXXXXXXXXXXXXXXX 60

Query: 61  XXXXXXPSIPVVDLFPSGDFPEGEIQQYKDDNLWRTTSEEKRELERLEKPIYNSVRRAAE 120
           XXXXXXPSIPVVDLFPSGD+PEGEIQQYKDDNLWRTTSEEKRELERLEKPIYNSVRRAAE
Sbjct: 61  XXXXXXPSIPVVDLFPSGDYPEGEIQQYKDDNLWRTTSEEKRELERLEKPIYNSVRRAAE 120

Query: 121 VHRQVRRYIKSILKPGMLMTDLCETLENTVRKLISENGLQAGIAFPTGCSLNWVAAHWTP 180
           VHRQVR+YIKSILKPGMLMTDLCETLENTVRKLISENGLQAGIAFPTGCSLNWVAAHWTP
Sbjct: 121 VHRQVRKYIKSILKPGMLMTDLCETLENTVRKLISENGLQAGIAFPTGCSLNWVAAHWTP 180

Query: 181 NTGDKT-------------------------------------------------ESGID 240
           NTGDKT                                                 ESGID
Sbjct: 181 NTGDKTVLGYNDVMKLDFGTHIDGCIVDXXXXXXXXXXXXXXXXXXXXXXNTGIKESGID 240

Query: 241 VRLCDV---------------------VKSIRNLNGHSIGRYQIHAGKSVPIVKGGEQTK 300
           VRLCDV                     VKSIRNLNGHSIGRYQIHAGKSVPIVKGGEQTK
Sbjct: 241 VRLCDVGAAIQEVMESYEVEINGKVFQVKSIRNLNGHSIGRYQIHAGKSVPIVKGGEQTK 300

Query: 301 MEEGEFFAIETFGSTGKGYVREDLECSHYMKNFDVGHIPLRLPRAKQLLATINKNFSTLA 355
           MEEGEFFAIETFGSTGKGYVREDLECSHYMKNFDVGHIPLRLPRAKQLLATIN NFSTLA
Sbjct: 301 MEEGEFFAIETFGSTGKGYVREDLECSHYMKNFDVGHIPLRLPRAKQLLATINNNFSTLA 360

BLAST of Carg07425-RA vs. NCBI nr
Match: XP_022998246.1 (methionine aminopeptidase 2B-like [Cucurbita maxima])

HSP 1 Score: 600.9 bits (1548), Expect = 2.8e-168
Identity = 344/424 (81.13%), Postives = 348/424 (82.08%), Query Frame = 0

Query: 1   MADENHTGIRVPEDGASEPSNGNEPDSVELASTSQKXXXXXXXXXXXXXXXXXXXXXXXX 60
           MADENH+ IRVPEDGAS PSNGNE DSVELASTS  XXXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 1   MADENHSEIRVPEDGASGPSNGNEQDSVELASTSXXXXXXXXXXXXXXXXXXXXXXXXXX 60

Query: 61  XXXXXXPSIPVVDLFPSGDFPEGEIQQYKDDNLWRTTSEEKRELERLEKPIYNSVRRAAE 120
           XXXXXX SIPVVDLFPSGDFPEGEIQ+YKDDNLWRTTSEEKRELERLEKPIYNSVRRAAE
Sbjct: 61  XXXXXXXSIPVVDLFPSGDFPEGEIQRYKDDNLWRTTSEEKRELERLEKPIYNSVRRAAE 120

Query: 121 VHRQVRRYIKSILKPGMLMTDLCETLENTVRKLISENGLQAGIAFPTGCSLNWVAAHWTP 180
           VHRQVRRYIKSILKPGMLMTDLCETLENTVRKLISENGLQAGIAFPTGCSLNWVAAHWTP
Sbjct: 121 VHRQVRRYIKSILKPGMLMTDLCETLENTVRKLISENGLQAGIAFPTGCSLNWVAAHWTP 180

Query: 181 NTGDKT-------------------------------------------------ESGID 240
           NTGDKT                                                 ESGID
Sbjct: 181 NTGDKTVLNYDDVMKLDFGTHIDGCIVDXXXXXXXXXXXXXXXXXXXXATNTGIKESGID 240

Query: 241 VRLCDV---------------------VKSIRNLNGHSIGRYQIHAGKSVPIVKGGEQTK 300
           VRLCD+                     VKSIRNLNGHSIGRYQIHAGKSVPIVKGGEQTK
Sbjct: 241 VRLCDIGAAIQEVMESYEVEINGTVFQVKSIRNLNGHSIGRYQIHAGKSVPIVKGGEQTK 300

Query: 301 MEEGEFFAIETFGSTGKGYVREDLECSHYMKNFDVGHIPLRLPRAKQLLATINKNFSTLA 355
           MEEGEFFAIETFGSTGKGYVREDLECSHYMKNFD+GHIPLRLPRAKQLLATINKNFSTLA
Sbjct: 301 MEEGEFFAIETFGSTGKGYVREDLECSHYMKNFDIGHIPLRLPRAKQLLATINKNFSTLA 360

BLAST of Carg07425-RA vs. NCBI nr
Match: XP_022934716.1 (methionine aminopeptidase 2B-like [Cucurbita moschata] >XP_022934717.1 methionine aminopeptidase 2B-like [Cucurbita moschata])

HSP 1 Score: 589.0 bits (1517), Expect = 1.1e-164
Identity = 329/424 (77.59%), Postives = 339/424 (79.95%), Query Frame = 0

Query: 1   MADENHTGIRVPEDGASEPSNGNEPDSVELASTSQKXXXXXXXXXXXXXXXXXXXXXXXX 60
           MADENH  + V EDGASEPSNGNE DSVELA  SQK      XXXXXXXXXXXXXXXXXX
Sbjct: 1   MADENHKEVPVHEDGASEPSNGNEQDSVELAPASQKDGDDVKXXXXXXXXXXXXXXXXXX 60

Query: 61  XXXXXXPSIPVVDLFPSGDFPEGEIQQYKDDNLWRTTSEEKRELERLEKPIYNSVRRAAE 120
           XXXXXXPSIP+VDLFPSGDFPEGEIQQYKDDNLWRTTSEEKRELERLEKP+YNSVRRAAE
Sbjct: 61  XXXXXXPSIPIVDLFPSGDFPEGEIQQYKDDNLWRTTSEEKRELERLEKPMYNSVRRAAE 120

Query: 121 VHRQVRRYIKSILKPGMLMTDLCETLENTVRKLISENGLQAGIAFPTGCSLNWVAAHWTP 180
           VHRQVR+YI+SI+KPGMLMTDLCETLENTVRKLISENGLQAGIAFPTGCSLNWVAAHWTP
Sbjct: 121 VHRQVRKYIRSIVKPGMLMTDLCETLENTVRKLISENGLQAGIAFPTGCSLNWVAAHWTP 180

Query: 181 NTGDKT-------------------------------------------------ESGID 240
           NTGDKT                                                 ESGID
Sbjct: 181 NTGDKTVLQYDDVMKLDFGTHIDGCIIDXXXXXXXXXXXXXXXXXXXXXXNTGIKESGID 240

Query: 241 VRLCDV---------------------VKSIRNLNGHSIGRYQIHAGKSVPIVKGGEQTK 300
           VRLCDV                     VKSIRNLNGHSIGRYQIHAGKSVPIVKGGEQTK
Sbjct: 241 VRLCDVGAAIQEVMESYEVEINGKVYQVKSIRNLNGHSIGRYQIHAGKSVPIVKGGEQTK 300

Query: 301 MEEGEFFAIETFGSTGKGYVREDLECSHYMKNFDVGHIPLRLPRAKQLLATINKNFSTLA 355
           MEEGEFFAIETFGSTGKGYVREDLECSHYMKNFDVGH+PLRLPRAKQLLATIN NFSTLA
Sbjct: 301 MEEGEFFAIETFGSTGKGYVREDLECSHYMKNFDVGHVPLRLPRAKQLLATINNNFSTLA 360

BLAST of Carg07425-RA vs. NCBI nr
Match: XP_022982549.1 (methionine aminopeptidase 2B [Cucurbita maxima] >XP_022982550.1 methionine aminopeptidase 2B [Cucurbita maxima])

HSP 1 Score: 587.8 bits (1514), Expect = 2.5e-164
Identity = 327/424 (77.12%), Postives = 338/424 (79.72%), Query Frame = 0

Query: 1   MADENHTGIRVPEDGASEPSNGNEPDSVELASTSQKXXXXXXXXXXXXXXXXXXXXXXXX 60
           MADENH  + V EDGASEPSNGN+ DSVELA  SQK      XXXXXXXXXXXXXXXXXX
Sbjct: 1   MADENHKEVPVHEDGASEPSNGNDQDSVELAPASQKDGDDVKXXXXXXXXXXXXXXXXXX 60

Query: 61  XXXXXXPSIPVVDLFPSGDFPEGEIQQYKDDNLWRTTSEEKRELERLEKPIYNSVRRAAE 120
           XXXXX PSIP+VDLFPSGDFPEGEIQQYKDDNLWRTTSEEKRELERLEKP+YNSVRRAAE
Sbjct: 61  XXXXXPPSIPIVDLFPSGDFPEGEIQQYKDDNLWRTTSEEKRELERLEKPMYNSVRRAAE 120

Query: 121 VHRQVRRYIKSILKPGMLMTDLCETLENTVRKLISENGLQAGIAFPTGCSLNWVAAHWTP 180
           VHRQVR+YI+SI+KPGMLMTDLCETLENTVRKLISENGLQAGIAFPTGCSLNWVAAHWTP
Sbjct: 121 VHRQVRKYIRSIVKPGMLMTDLCETLENTVRKLISENGLQAGIAFPTGCSLNWVAAHWTP 180

Query: 181 NTGDKT-------------------------------------------------ESGID 240
           NTGDKT                                                 ESGID
Sbjct: 181 NTGDKTVLQYDDVMKLDFGTHIDGCIIDXXXXXXXXXXXXXXXXXXXXXXNTGIKESGID 240

Query: 241 VRLCDV---------------------VKSIRNLNGHSIGRYQIHAGKSVPIVKGGEQTK 300
           VRLCDV                     VKSIRNLNGHSIGRYQIHAGKSVPIVKGGEQTK
Sbjct: 241 VRLCDVGAAIQEVMESYEVEINGKVYQVKSIRNLNGHSIGRYQIHAGKSVPIVKGGEQTK 300

Query: 301 MEEGEFFAIETFGSTGKGYVREDLECSHYMKNFDVGHIPLRLPRAKQLLATINKNFSTLA 355
           MEEGEFFAIETFGSTGKGYVREDLECSHYMKNFDVGH+PLRLPRAKQLLATIN NFSTLA
Sbjct: 301 MEEGEFFAIETFGSTGKGYVREDLECSHYMKNFDVGHVPLRLPRAKQLLATINNNFSTLA 360

BLAST of Carg07425-RA vs. TAIR10
Match: AT3G59990.1 (methionine aminopeptidase 2B)

HSP 1 Score: 534.3 bits (1375), Expect = 5.8e-152
Identity = 265/358 (74.02%), Postives = 282/358 (78.77%), Query Frame = 0

Query: 67  PSIPVVDLFPSGDFPEGEIQQYKDDNLWRTTSEEKRELERLEKPIYNSVRRAAEVHRQVR 126
           PSIPVV+LFPSG+FPEGEIQ+YKDDNLWRTTSEEKRELER EKPIYNSVRRAAEVHRQVR
Sbjct: 82  PSIPVVELFPSGEFPEGEIQEYKDDNLWRTTSEEKRELERFEKPIYNSVRRAAEVHRQVR 141

Query: 127 RYIKSILKPGMLMTDLCETLENTVRKLISENGLQAGIAFPTGCSLNWVAAHWTPNTGDKT 186
           +Y++SI+KPGMLMTD+CETLENTVRKLISENGLQAGIAFPTGCSLNWVAAHWTPN+GDKT
Sbjct: 142 KYVRSIVKPGMLMTDICETLENTVRKLISENGLQAGIAFPTGCSLNWVAAHWTPNSGDKT 201

Query: 187 -------------------------------------------------ESGIDVRLCDV 246
                                                            E+GIDVRLCD+
Sbjct: 202 VLQYDDVMKLDFGTHIDGHIIDCAFTVAFNPMFDPLLAASREATYTGIKEAGIDVRLCDI 261

Query: 247 ---------------------VKSIRNLNGHSIGRYQIHAGKSVPIVKGGEQTKMEEGEF 306
                                VKSIRNLNGHSIG YQIHAGKSVPIVKGGEQTKMEEGEF
Sbjct: 262 GAAIQEVMESYEVEINGKVFQVKSIRNLNGHSIGPYQIHAGKSVPIVKGGEQTKMEEGEF 321

Query: 307 FAIETFGSTGKGYVREDLECSHYMKNFDVGHIPLRLPRAKQLLATINKNFSTLAFCKRYL 355
           +AIETFGSTGKGYVREDLECSHYMKNFD GH+PLRLPRAKQLLATINKNFSTLAFC+RYL
Sbjct: 322 YAIETFGSTGKGYVREDLECSHYMKNFDAGHVPLRLPRAKQLLATINKNFSTLAFCRRYL 381

BLAST of Carg07425-RA vs. TAIR10
Match: AT2G44180.1 (methionine aminopeptidase 2A)

HSP 1 Score: 511.1 bits (1315), Expect = 5.3e-145
Identity = 254/358 (70.95%), Postives = 278/358 (77.65%), Query Frame = 0

Query: 67  PSIPVVDLFPSGDFPEGEIQQYKDDNLWRTTSEEKRELERLEKPIYNSVRRAAEVHRQVR 126
           PSIPV++LFPSGDFP+GEIQQY DDNLWRTTSEEKRE+ERL+KPIYNS+R+AAEVHRQVR
Sbjct: 84  PSIPVLELFPSGDFPQGEIQQYNDDNLWRTTSEEKREMERLQKPIYNSLRQAAEVHRQVR 143

Query: 127 RYIKSILKPGMLMTDLCETLENTVRKLISENGLQAGIAFPTGCSLNWVAAHWTPNTGDKT 186
           +Y++SILKPGMLM DLCETLENTVRKLISENGLQAGIAFPTGCSLN VAAHWTPN+GDKT
Sbjct: 144 KYMRSILKPGMLMIDLCETLENTVRKLISENGLQAGIAFPTGCSLNNVAAHWTPNSGDKT 203

Query: 187 -------------------------------------------------ESGIDVRLCDV 246
                                                            E+G+DVRLCDV
Sbjct: 204 VLQYDDVMKLDFGTHIDGHIVDSAFTVAFNPMFDPLLAASRDATYTGIKEAGVDVRLCDV 263

Query: 247 ---------------------VKSIRNLNGHSIGRYQIHAGKSVPIVKGGEQTKMEEGEF 306
                                VKSIRNLNGHSIGRYQIHA KSVP V+GGEQTKMEEGE 
Sbjct: 264 GAAVQEVMESYEVEINGKVYQVKSIRNLNGHSIGRYQIHAEKSVPNVRGGEQTKMEEGEL 323

Query: 307 FAIETFGSTGKGYVREDLECSHYMKNFDVGHIPLRLPRAKQLLATINKNFSTLAFCKRYL 355
           +AIETFGSTGKGYVREDLECSHYMKN+DVGH+PLRLPRAKQLLATINKNFSTLAFC+RYL
Sbjct: 324 YAIETFGSTGKGYVREDLECSHYMKNYDVGHVPLRLPRAKQLLATINKNFSTLAFCRRYL 383

BLAST of Carg07425-RA vs. Swiss-Prot
Match: sp|Q56Y85|MAP22_ARATH (Methionine aminopeptidase 2B OS=Arabidopsis thaliana OX=3702 GN=MAP2B PE=2 SV=2)

HSP 1 Score: 534.3 bits (1375), Expect = 1.1e-150
Identity = 265/358 (74.02%), Postives = 282/358 (78.77%), Query Frame = 0

Query: 67  PSIPVVDLFPSGDFPEGEIQQYKDDNLWRTTSEEKRELERLEKPIYNSVRRAAEVHRQVR 126
           PSIPVV+LFPSG+FPEGEIQ+YKDDNLWRTTSEEKRELER EKPIYNSVRRAAEVHRQVR
Sbjct: 82  PSIPVVELFPSGEFPEGEIQEYKDDNLWRTTSEEKRELERFEKPIYNSVRRAAEVHRQVR 141

Query: 127 RYIKSILKPGMLMTDLCETLENTVRKLISENGLQAGIAFPTGCSLNWVAAHWTPNTGDKT 186
           +Y++SI+KPGMLMTD+CETLENTVRKLISENGLQAGIAFPTGCSLNWVAAHWTPN+GDKT
Sbjct: 142 KYVRSIVKPGMLMTDICETLENTVRKLISENGLQAGIAFPTGCSLNWVAAHWTPNSGDKT 201

Query: 187 -------------------------------------------------ESGIDVRLCDV 246
                                                            E+GIDVRLCD+
Sbjct: 202 VLQYDDVMKLDFGTHIDGHIIDCAFTVAFNPMFDPLLAASREATYTGIKEAGIDVRLCDI 261

Query: 247 ---------------------VKSIRNLNGHSIGRYQIHAGKSVPIVKGGEQTKMEEGEF 306
                                VKSIRNLNGHSIG YQIHAGKSVPIVKGGEQTKMEEGEF
Sbjct: 262 GAAIQEVMESYEVEINGKVFQVKSIRNLNGHSIGPYQIHAGKSVPIVKGGEQTKMEEGEF 321

Query: 307 FAIETFGSTGKGYVREDLECSHYMKNFDVGHIPLRLPRAKQLLATINKNFSTLAFCKRYL 355
           +AIETFGSTGKGYVREDLECSHYMKNFD GH+PLRLPRAKQLLATINKNFSTLAFC+RYL
Sbjct: 322 YAIETFGSTGKGYVREDLECSHYMKNFDAGHVPLRLPRAKQLLATINKNFSTLAFCRRYL 381

BLAST of Carg07425-RA vs. Swiss-Prot
Match: sp|Q9FV49|MAP21_ARATH (Methionine aminopeptidase 2A OS=Arabidopsis thaliana OX=3702 GN=MAP2A PE=2 SV=2)

HSP 1 Score: 511.1 bits (1315), Expect = 9.6e-144
Identity = 254/358 (70.95%), Postives = 278/358 (77.65%), Query Frame = 0

Query: 67  PSIPVVDLFPSGDFPEGEIQQYKDDNLWRTTSEEKRELERLEKPIYNSVRRAAEVHRQVR 126
           PSIPV++LFPSGDFP+GEIQQY DDNLWRTTSEEKRE+ERL+KPIYNS+R+AAEVHRQVR
Sbjct: 84  PSIPVLELFPSGDFPQGEIQQYNDDNLWRTTSEEKREMERLQKPIYNSLRQAAEVHRQVR 143

Query: 127 RYIKSILKPGMLMTDLCETLENTVRKLISENGLQAGIAFPTGCSLNWVAAHWTPNTGDKT 186
           +Y++SILKPGMLM DLCETLENTVRKLISENGLQAGIAFPTGCSLN VAAHWTPN+GDKT
Sbjct: 144 KYMRSILKPGMLMIDLCETLENTVRKLISENGLQAGIAFPTGCSLNNVAAHWTPNSGDKT 203

Query: 187 -------------------------------------------------ESGIDVRLCDV 246
                                                            E+G+DVRLCDV
Sbjct: 204 VLQYDDVMKLDFGTHIDGHIVDSAFTVAFNPMFDPLLAASRDATYTGIKEAGVDVRLCDV 263

Query: 247 ---------------------VKSIRNLNGHSIGRYQIHAGKSVPIVKGGEQTKMEEGEF 306
                                VKSIRNLNGHSIGRYQIHA KSVP V+GGEQTKMEEGE 
Sbjct: 264 GAAVQEVMESYEVEINGKVYQVKSIRNLNGHSIGRYQIHAEKSVPNVRGGEQTKMEEGEL 323

Query: 307 FAIETFGSTGKGYVREDLECSHYMKNFDVGHIPLRLPRAKQLLATINKNFSTLAFCKRYL 355
           +AIETFGSTGKGYVREDLECSHYMKN+DVGH+PLRLPRAKQLLATINKNFSTLAFC+RYL
Sbjct: 324 YAIETFGSTGKGYVREDLECSHYMKNYDVGHVPLRLPRAKQLLATINKNFSTLAFCRRYL 383

BLAST of Carg07425-RA vs. Swiss-Prot
Match: sp|P50579|MAP2_HUMAN (Methionine aminopeptidase 2 OS=Homo sapiens OX=9606 GN=METAP2 PE=1 SV=1)

HSP 1 Score: 407.1 bits (1045), Expect = 1.9e-112
Identity = 205/363 (56.47%), Postives = 241/363 (66.39%), Query Frame = 0

Query: 67  PSIPVVDLFPSGDFPEGEIQQYKDDN-----LWRTTSEEKRELERLEKPIYNSVRRAAEV 126
           PS+P+ DL+P+G FP+G+  +Y          WRTTSEEK+ L++  + I+N  R AAE 
Sbjct: 116 PSVPICDLYPNGVFPKGQECEYPPTQDGRTAAWRTTSEEKKALDQASEEIWNDFREAAEA 175

Query: 127 HRQVRRYIKSILKPGMLMTDLCETLENTVRKLISENGLQAGIAFPTGCSLNWVAAHWTPN 186
           HRQVR+Y+ S +KPGM M ++CE LE+  RKLI ENGL AG+AFPTGCSLN  AAH+TPN
Sbjct: 176 HRQVRKYVMSWIKPGMTMIEICEKLEDCSRKLIKENGLNAGLAFPTGCSLNNCAAHYTPN 235

Query: 187 TGDKTE-------------------------------------------------SGIDV 246
            GD T                                                  +GIDV
Sbjct: 236 AGDTTVLQYDDICKIDFGTHISGRIIDCAFTVTFNPKYDTLLKAVKDATNTGIKCAGIDV 295

Query: 247 RLCDV---------------------VKSIRNLNGHSIGRYQIHAGKSVPIVKGGEQTKM 306
           RLCDV                     VK IRNLNGHSIG+Y+IHAGK+VPIVKGGE T+M
Sbjct: 296 RLCDVGEAIQEVMESYEVEIDGKTYQVKPIRNLNGHSIGQYRIHAGKTVPIVKGGEATRM 355

Query: 307 EEGEFFAIETFGSTGKGYVREDLECSHYMKNFDVGHIPLRLPRAKQLLATINKNFSTLAF 355
           EEGE +AIETFGSTGKG V +D+ECSHYMKNFDVGH+P+RLPR K LL  IN+NF TLAF
Sbjct: 356 EEGEVYAIETFGSTGKGVVHDDMECSHYMKNFDVGHVPIRLPRTKHLLNVINENFGTLAF 415

BLAST of Carg07425-RA vs. Swiss-Prot
Match: sp|O08663|MAP2_MOUSE (Methionine aminopeptidase 2 OS=Mus musculus OX=10090 GN=Metap2 PE=1 SV=1)

HSP 1 Score: 406.0 bits (1042), Expect = 4.3e-112
Identity = 205/363 (56.47%), Postives = 240/363 (66.12%), Query Frame = 0

Query: 67  PSIPVVDLFPSGDFPEGEIQQYKDDN-----LWRTTSEEKRELERLEKPIYNSVRRAAEV 126
           PS+P+ DL+P+G FP+G+  +Y          WRTTSEEK+ L++  + I+N  R AAE 
Sbjct: 116 PSVPICDLYPNGVFPKGQECEYPPTQDGRTAAWRTTSEEKKALDQASEEIWNDFREAAEA 175

Query: 127 HRQVRRYIKSILKPGMLMTDLCETLENTVRKLISENGLQAGIAFPTGCSLNWVAAHWTPN 186
           HRQVR+Y+ S +KPGM M ++CE LE+  RKLI ENGL AG+AFPTGCSLN  AAH+TPN
Sbjct: 176 HRQVRKYVMSWIKPGMTMIEICEKLEDCSRKLIKENGLNAGLAFPTGCSLNNCAAHYTPN 235

Query: 187 TGDKTE-------------------------------------------------SGIDV 246
            GD T                                                  +GIDV
Sbjct: 236 AGDTTVLQYDDICKIDFGTHISGRIIDCAFTVTFNPKYDILLTAVKDATNTGIKCAGIDV 295

Query: 247 RLCDV---------------------VKSIRNLNGHSIGRYQIHAGKSVPIVKGGEQTKM 306
           RLCDV                     VK IRNLNGHSIG Y+IHAGK+VPIVKGGE T+M
Sbjct: 296 RLCDVGEAIQEVMESYEVEIDGKTYQVKPIRNLNGHSIGPYRIHAGKTVPIVKGGEATRM 355

Query: 307 EEGEFFAIETFGSTGKGYVREDLECSHYMKNFDVGHIPLRLPRAKQLLATINKNFSTLAF 355
           EEGE +AIETFGSTGKG V +D+ECSHYMKNFDVGH+P+RLPR K LL  IN+NF TLAF
Sbjct: 356 EEGEVYAIETFGSTGKGVVHDDMECSHYMKNFDVGHVPIRLPRTKHLLNVINENFGTLAF 415

BLAST of Carg07425-RA vs. Swiss-Prot
Match: sp|P38062|MAP2_RAT (Methionine aminopeptidase 2 OS=Rattus norvegicus OX=10116 GN=Metap2 PE=1 SV=2)

HSP 1 Score: 406.0 bits (1042), Expect = 4.3e-112
Identity = 205/363 (56.47%), Postives = 240/363 (66.12%), Query Frame = 0

Query: 67  PSIPVVDLFPSGDFPEGEIQQYKDDN-----LWRTTSEEKRELERLEKPIYNSVRRAAEV 126
           PS+P+ DL+P+G FP+G+  +Y          WRTTSEEK+ L++  + I+N  R AAE 
Sbjct: 116 PSVPICDLYPNGVFPKGQECEYPPTQDGRTAAWRTTSEEKKALDQASEEIWNDFREAAEA 175

Query: 127 HRQVRRYIKSILKPGMLMTDLCETLENTVRKLISENGLQAGIAFPTGCSLNWVAAHWTPN 186
           HRQVR+Y+ S +KPGM M ++CE LE+  RKLI ENGL AG+AFPTGCSLN  AAH+TPN
Sbjct: 176 HRQVRKYVMSWIKPGMTMIEICEKLEDCSRKLIKENGLNAGLAFPTGCSLNNCAAHYTPN 235

Query: 187 TGDKTE-------------------------------------------------SGIDV 246
            GD T                                                  +GIDV
Sbjct: 236 AGDTTVLQYDDICKIDFGTHISGRIIDCAFTVTFNPKYDILLKAVKDATNTGIKCAGIDV 295

Query: 247 RLCDV---------------------VKSIRNLNGHSIGRYQIHAGKSVPIVKGGEQTKM 306
           RLCDV                     VK IRNLNGHSIG Y+IHAGK+VPIVKGGE T+M
Sbjct: 296 RLCDVGEAIQEVMESYEVEIDGKTYQVKPIRNLNGHSIGPYRIHAGKTVPIVKGGEATRM 355

Query: 307 EEGEFFAIETFGSTGKGYVREDLECSHYMKNFDVGHIPLRLPRAKQLLATINKNFSTLAF 355
           EEGE +AIETFGSTGKG V +D+ECSHYMKNFDVGH+P+RLPR K LL  IN+NF TLAF
Sbjct: 356 EEGEVYAIETFGSTGKGVVHDDMECSHYMKNFDVGHVPIRLPRTKHLLNVINENFGTLAF 415

BLAST of Carg07425-RA vs. TrEMBL
Match: tr|A0A1S4E1G4|A0A1S4E1G4_CUCME (Methionine aminopeptidase 2 OS=Cucumis melo OX=3656 GN=LOC103496871 PE=3 SV=1)

HSP 1 Score: 580.9 bits (1496), Expect = 2.0e-162
Identity = 318/424 (75.00%), Postives = 332/424 (78.30%), Query Frame = 0

Query: 1   MADENHTGIRVPEDGASEPSNGNEPDSVELASTSQKXXXXXXXXXXXXXXXXXXXXXXXX 60
           MADENH  + VP+DGA E SNG+E DSVELA TSQK        XXXXXXXXXXXXXXXX
Sbjct: 13  MADENHIEVPVPDDGAPETSNGHEQDSVELAPTSQKGEDDVKEGXXXXXXXXXXXXXXXX 72

Query: 61  XXXXXXPSIPVVDLFPSGDFPEGEIQQYKDDNLWRTTSEEKRELERLEKPIYNSVRRAAE 120
           XXX   P+IP++DLFPSGDFPEGEIQQYKDDNLWRTTSEEKRELERLEKPIYNS+RRAAE
Sbjct: 73  XXXTDPPTIPIIDLFPSGDFPEGEIQQYKDDNLWRTTSEEKRELERLEKPIYNSIRRAAE 132

Query: 121 VHRQVRRYIKSILKPGMLMTDLCETLENTVRKLISENGLQAGIAFPTGCSLNWVAAHWTP 180
           VHRQVR+YI+SI+KPGMLMTDLCETLENTVRKLI ENGLQAGIAFPTGCSLNWVAAHWTP
Sbjct: 133 VHRQVRKYIRSIVKPGMLMTDLCETLENTVRKLILENGLQAGIAFPTGCSLNWVAAHWTP 192

Query: 181 NTGDKT-------------------------------------------------ESGID 240
           NTGD T                                                 ESGID
Sbjct: 193 NTGDTTVLQYDDVMKLDFGTHIDGCIVDXXXXXXXXXXXXXXXXXXXXXXNTGIKESGID 252

Query: 241 VRLCDV---------------------VKSIRNLNGHSIGRYQIHAGKSVPIVKGGEQTK 300
           VRLCDV                     VKSIRNLNGHSIGRYQIHAGKSVPIVKGGEQTK
Sbjct: 253 VRLCDVGAAIQEVMESYEVEINGKVYQVKSIRNLNGHSIGRYQIHAGKSVPIVKGGEQTK 312

Query: 301 MEEGEFFAIETFGSTGKGYVREDLECSHYMKNFDVGHIPLRLPRAKQLLATINKNFSTLA 355
           MEEGEFFAIETFGSTGKGYVREDLECSHYMKNFDVGH+PLRLPRAKQLLATIN NFSTLA
Sbjct: 313 MEEGEFFAIETFGSTGKGYVREDLECSHYMKNFDVGHVPLRLPRAKQLLATINNNFSTLA 372

BLAST of Carg07425-RA vs. TrEMBL
Match: tr|A0A1S3C5H0|A0A1S3C5H0_CUCME (Methionine aminopeptidase 2 OS=Cucumis melo OX=3656 GN=LOC103496871 PE=3 SV=1)

HSP 1 Score: 580.9 bits (1496), Expect = 2.0e-162
Identity = 318/424 (75.00%), Postives = 332/424 (78.30%), Query Frame = 0

Query: 1   MADENHTGIRVPEDGASEPSNGNEPDSVELASTSQKXXXXXXXXXXXXXXXXXXXXXXXX 60
           MADENH  + VP+DGA E SNG+E DSVELA TSQK        XXXXXXXXXXXXXXXX
Sbjct: 1   MADENHIEVPVPDDGAPETSNGHEQDSVELAPTSQKGEDDVKEGXXXXXXXXXXXXXXXX 60

Query: 61  XXXXXXPSIPVVDLFPSGDFPEGEIQQYKDDNLWRTTSEEKRELERLEKPIYNSVRRAAE 120
           XXX   P+IP++DLFPSGDFPEGEIQQYKDDNLWRTTSEEKRELERLEKPIYNS+RRAAE
Sbjct: 61  XXXTDPPTIPIIDLFPSGDFPEGEIQQYKDDNLWRTTSEEKRELERLEKPIYNSIRRAAE 120

Query: 121 VHRQVRRYIKSILKPGMLMTDLCETLENTVRKLISENGLQAGIAFPTGCSLNWVAAHWTP 180
           VHRQVR+YI+SI+KPGMLMTDLCETLENTVRKLI ENGLQAGIAFPTGCSLNWVAAHWTP
Sbjct: 121 VHRQVRKYIRSIVKPGMLMTDLCETLENTVRKLILENGLQAGIAFPTGCSLNWVAAHWTP 180

Query: 181 NTGDKT-------------------------------------------------ESGID 240
           NTGD T                                                 ESGID
Sbjct: 181 NTGDTTVLQYDDVMKLDFGTHIDGCIVDXXXXXXXXXXXXXXXXXXXXXXNTGIKESGID 240

Query: 241 VRLCDV---------------------VKSIRNLNGHSIGRYQIHAGKSVPIVKGGEQTK 300
           VRLCDV                     VKSIRNLNGHSIGRYQIHAGKSVPIVKGGEQTK
Sbjct: 241 VRLCDVGAAIQEVMESYEVEINGKVYQVKSIRNLNGHSIGRYQIHAGKSVPIVKGGEQTK 300

Query: 301 MEEGEFFAIETFGSTGKGYVREDLECSHYMKNFDVGHIPLRLPRAKQLLATINKNFSTLA 355
           MEEGEFFAIETFGSTGKGYVREDLECSHYMKNFDVGH+PLRLPRAKQLLATIN NFSTLA
Sbjct: 301 MEEGEFFAIETFGSTGKGYVREDLECSHYMKNFDVGHVPLRLPRAKQLLATINNNFSTLA 360

BLAST of Carg07425-RA vs. TrEMBL
Match: tr|A0A0A0L674|A0A0A0L674_CUCSA (Methionine aminopeptidase 2 OS=Cucumis sativus OX=3659 GN=Csa_3G189860 PE=3 SV=1)

HSP 1 Score: 580.9 bits (1496), Expect = 2.0e-162
Identity = 321/424 (75.71%), Postives = 335/424 (79.01%), Query Frame = 0

Query: 1   MADENHTGIRVPEDGASEPSNGNEPDSVELASTSQKXXXXXXXXXXXXXXXXXXXXXXXX 60
           MADENH  + VP+DGA E SNG+E DSVELA TSQK        XXXXXXXXXXXXXXXX
Sbjct: 1   MADENHIEVPVPDDGAPETSNGHEQDSVELAPTSQKGEDDVKEGXXXXXXXXXXXXXXXX 60

Query: 61  XXXXXXPSIPVVDLFPSGDFPEGEIQQYKDDNLWRTTSEEKRELERLEKPIYNSVRRAAE 120
           XXXXXXP+IP+ DLFPSGDFPEGEIQQYKDDNLWRTTSEEKRELERLEKP+YNS+RRAAE
Sbjct: 61  XXXXXXPTIPITDLFPSGDFPEGEIQQYKDDNLWRTTSEEKRELERLEKPMYNSIRRAAE 120

Query: 121 VHRQVRRYIKSILKPGMLMTDLCETLENTVRKLISENGLQAGIAFPTGCSLNWVAAHWTP 180
           VHRQVR+YI+SI+KPGMLMTDLCETLENTVRKLISENGLQAGIAFPTGCSLNWVAAHWTP
Sbjct: 121 VHRQVRKYIRSIVKPGMLMTDLCETLENTVRKLISENGLQAGIAFPTGCSLNWVAAHWTP 180

Query: 181 NTGDKT-------------------------------------------------ESGID 240
           NTGD T                                                 ESGID
Sbjct: 181 NTGDTTVLQYDDVMKLDFGTHIDGCIVDXXXXXXXXXXXXXXXXXXXXXXNTGIKESGID 240

Query: 241 VRLCDV---------------------VKSIRNLNGHSIGRYQIHAGKSVPIVKGGEQTK 300
           VRLCDV                     VKSIRNLNGHSIGRYQIHAGKSVPIVKGGEQTK
Sbjct: 241 VRLCDVGAAIQEVMESYEVEINGKVYQVKSIRNLNGHSIGRYQIHAGKSVPIVKGGEQTK 300

Query: 301 MEEGEFFAIETFGSTGKGYVREDLECSHYMKNFDVGHIPLRLPRAKQLLATINKNFSTLA 355
           MEEGEFFAIETFGSTGKGYVREDLECSHYMKNFDVGH+PLRLPRAKQLLATIN NFSTLA
Sbjct: 301 MEEGEFFAIETFGSTGKGYVREDLECSHYMKNFDVGHVPLRLPRAKQLLATINNNFSTLA 360

BLAST of Carg07425-RA vs. TrEMBL
Match: tr|V4TZ76|V4TZ76_9ROSI (Methionine aminopeptidase 2 OS=Citrus clementina OX=85681 GN=CICLE_v10020270mg PE=3 SV=1)

HSP 1 Score: 543.5 bits (1399), Expect = 3.5e-151
Identity = 317/425 (74.59%), Postives = 327/425 (76.94%), Query Frame = 0

Query: 1   MADEN-HTGIRVPEDGASEPSNGNEPDSVELASTSQKXXXXXXXXXXXXXXXXXXXXXXX 60
           MADEN    I + E+G  E  NGNE  S         XXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 1   MADENVSKEISIEENGTLESVNGNEEHSDVSXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 60

Query: 61  XXXXXXXPSIPVVDLFPSGDFPEGEIQQYKDDNLWRTTSEEKRELERLEKPIYNSVRRAA 120
           XXXXX  PSIPVVDLFPSG+FPEGEIQQYKDDNLWRTTSEEKRELERLEKP YNSVR+AA
Sbjct: 61  XXXXXDPPSIPVVDLFPSGEFPEGEIQQYKDDNLWRTTSEEKRELERLEKPKYNSVRQAA 120

Query: 121 EVHRQVRRYIKSILKPGMLMTDLCETLENTVRKLISENGLQAGIAFPTGCSLNWVAAHWT 180
           EVHRQVR+YIKSILKPGMLMTDLCETLENTVRKLISENGLQAGIAFPTGCSLNWVAAHWT
Sbjct: 121 EVHRQVRKYIKSILKPGMLMTDLCETLENTVRKLISENGLQAGIAFPTGCSLNWVAAHWT 180

Query: 181 PNTGDKT-------------------------------------------------ESGI 240
           PN+GDKT                                                 E+GI
Sbjct: 181 PNSGDKTVLQYDDVMKLDFGTHIDGCIVDXXXXXXXXXXXXXXXXXXXXXXNTGIKEAGI 240

Query: 241 DVRLCDV---------------------VKSIRNLNGHSIGRYQIHAGKSVPIVKGGEQT 300
           DVRLCD+                     VKSIRNLNGHSIGRYQIHAGKSVPIVKGGEQT
Sbjct: 241 DVRLCDIGAAIQEVMESYEVEINGKVFQVKSIRNLNGHSIGRYQIHAGKSVPIVKGGEQT 300

Query: 301 KMEEGEFFAIETFGSTGKGYVREDLECSHYMKNFDVGHIPLRLPRAKQLLATINKNFSTL 355
           KMEEGEFFAIETF STGKGYVREDLECSHYMKNFDVGHIPLRLPRAKQLLATINKNFSTL
Sbjct: 301 KMEEGEFFAIETFASTGKGYVREDLECSHYMKNFDVGHIPLRLPRAKQLLATINKNFSTL 360

BLAST of Carg07425-RA vs. TrEMBL
Match: tr|A0A2C9WH03|A0A2C9WH03_MANES (Methionine aminopeptidase 2 OS=Manihot esculenta OX=3983 GN=MANES_01G007800 PE=3 SV=1)

HSP 1 Score: 543.1 bits (1398), Expect = 4.6e-151
Identity = 275/358 (76.82%), Postives = 282/358 (78.77%), Query Frame = 0

Query: 67  PSIPVVDLFPSGDFPEGEIQQYKDDNLWRTTSEEKRELERLEKPIYNSVRRAAEVHRQVR 126
           PSIPV++LFPSG+FPEGEIQQYKDDNLWRTTSEEKRELERLEKPIYNSVR+AAEVHRQVR
Sbjct: 75  PSIPVIELFPSGEFPEGEIQQYKDDNLWRTTSEEKRELERLEKPIYNSVRQAAEVHRQVR 134

Query: 127 RYIKSILKPGMLMTDLCETLENTVRKLISENGLQAGIAFPTGCSLNWVAAHWTPNTGDKT 186
           +YIK ILKPGMLMTDLCETLENTVRKLISENGLQAGIAFPTGCSLNWVAAHWTPN+GDKT
Sbjct: 135 KYIKGILKPGMLMTDLCETLENTVRKLISENGLQAGIAFPTGCSLNWVAAHWTPNSGDKT 194

Query: 187 -------------------------------------------------ESGIDVRLCDV 246
                                                            ESGIDVRLCDV
Sbjct: 195 VLQYDDVMKLDFGTHIDGYIVDCAFTVAFNPMFXXLLEASREATNTGIKESGIDVRLCDV 254

Query: 247 ---------------------VKSIRNLNGHSIGRYQIHAGKSVPIVKGGEQTKMEEGEF 306
                                VKSIRNLNGHSIGRYQIHAGKSVPIVKGGEQTKMEEGEF
Sbjct: 255 GAAIQEVMESYEVEINGKVFQVKSIRNLNGHSIGRYQIHAGKSVPIVKGGEQTKMEEGEF 314

Query: 307 FAIETFGSTGKGYVREDLECSHYMKNFDVGHIPLRLPRAKQLLATINKNFSTLAFCKRYL 355
           FAIETF STGKGYVREDLECSHYMKNFD GHIPLRLPRAKQLLATINKNFSTLAFC+RYL
Sbjct: 315 FAIETFASTGKGYVREDLECSHYMKNFDAGHIPLRLPRAKQLLATINKNFSTLAFCRRYL 374

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022948436.11.0e-17383.25methionine aminopeptidase 2B isoform X1 [Cucurbita moschata][more]
XP_023521739.11.6e-17182.31methionine aminopeptidase 2B isoform X1 [Cucurbita pepo subsp. pepo] >XP_0235217... [more]
XP_022998246.12.8e-16881.13methionine aminopeptidase 2B-like [Cucurbita maxima][more]
XP_022934716.11.1e-16477.59methionine aminopeptidase 2B-like [Cucurbita moschata] >XP_022934717.1 methionin... [more]
XP_022982549.12.5e-16477.12methionine aminopeptidase 2B [Cucurbita maxima] >XP_022982550.1 methionine amino... [more]
Match NameE-valueIdentityDescription
AT3G59990.15.8e-15274.02methionine aminopeptidase 2B[more]
AT2G44180.15.3e-14570.95methionine aminopeptidase 2A[more]
Match NameE-valueIdentityDescription
sp|Q56Y85|MAP22_ARATH1.1e-15074.02Methionine aminopeptidase 2B OS=Arabidopsis thaliana OX=3702 GN=MAP2B PE=2 SV=2[more]
sp|Q9FV49|MAP21_ARATH9.6e-14470.95Methionine aminopeptidase 2A OS=Arabidopsis thaliana OX=3702 GN=MAP2A PE=2 SV=2[more]
sp|P50579|MAP2_HUMAN1.9e-11256.47Methionine aminopeptidase 2 OS=Homo sapiens OX=9606 GN=METAP2 PE=1 SV=1[more]
sp|O08663|MAP2_MOUSE4.3e-11256.47Methionine aminopeptidase 2 OS=Mus musculus OX=10090 GN=Metap2 PE=1 SV=1[more]
sp|P38062|MAP2_RAT4.3e-11256.47Methionine aminopeptidase 2 OS=Rattus norvegicus OX=10116 GN=Metap2 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
tr|A0A1S4E1G4|A0A1S4E1G4_CUCME2.0e-16275.00Methionine aminopeptidase 2 OS=Cucumis melo OX=3656 GN=LOC103496871 PE=3 SV=1[more]
tr|A0A1S3C5H0|A0A1S3C5H0_CUCME2.0e-16275.00Methionine aminopeptidase 2 OS=Cucumis melo OX=3656 GN=LOC103496871 PE=3 SV=1[more]
tr|A0A0A0L674|A0A0A0L674_CUCSA2.0e-16275.71Methionine aminopeptidase 2 OS=Cucumis sativus OX=3659 GN=Csa_3G189860 PE=3 SV=1[more]
tr|V4TZ76|V4TZ76_9ROSI3.5e-15174.59Methionine aminopeptidase 2 OS=Citrus clementina OX=85681 GN=CICLE_v10020270mg P... [more]
tr|A0A2C9WH03|A0A2C9WH03_MANES4.6e-15176.82Methionine aminopeptidase 2 OS=Manihot esculenta OX=3983 GN=MANES_01G007800 PE=3... [more]
The following terms have been associated with this mRNA:
Vocabulary: Biological Process
TermDefinition
GO:0006508proteolysis
Vocabulary: Molecular Function
TermDefinition
GO:0004177aminopeptidase activity
GO:0008235metalloexopeptidase activity
Vocabulary: INTERPRO
TermDefinition
IPR036390WH_DNA-bd_sf
IPR036005Creatinase/aminopeptidase-like
IPR002468Pept_M24A_MAP2
IPR000994Pept_M24
IPR036388WH-like_DNA-bd_sf
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0004177 aminopeptidase activity
molecular_function GO:0008235 metalloexopeptidase activity
molecular_function GO:0003674 molecular_function

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Carg07425Carg07425gene


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Carg07425-RACarg07425-RA-proteinpolypeptide


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg07425-RA.exon.1Carg07425-RA.exon.1exon
Carg07425-RA.exon.2Carg07425-RA.exon.2exon
Carg07425-RA.exon.3Carg07425-RA.exon.3exon
Carg07425-RA.exon.4Carg07425-RA.exon.4exon
Carg07425-RA.exon.5Carg07425-RA.exon.5exon
Carg07425-RA.exon.6Carg07425-RA.exon.6exon
Carg07425-RA.exon.7Carg07425-RA.exon.7exon
Carg07425-RA.exon.8Carg07425-RA.exon.8exon
Carg07425-RA.exon.9Carg07425-RA.exon.9exon
Carg07425-RA.exon.10Carg07425-RA.exon.10exon
Carg07425-RA.exon.11Carg07425-RA.exon.11exon


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg07425-RA.five_prime_UTR.1Carg07425-RA.five_prime_UTR.1five_prime_UTR
Carg07425-RA.five_prime_UTR.2Carg07425-RA.five_prime_UTR.2five_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg07425-RA.CDS.1Carg07425-RA.CDS.1CDS
Carg07425-RA.CDS.2Carg07425-RA.CDS.2CDS
Carg07425-RA.CDS.3Carg07425-RA.CDS.3CDS
Carg07425-RA.CDS.4Carg07425-RA.CDS.4CDS
Carg07425-RA.CDS.5Carg07425-RA.CDS.5CDS
Carg07425-RA.CDS.6Carg07425-RA.CDS.6CDS
Carg07425-RA.CDS.7Carg07425-RA.CDS.7CDS
Carg07425-RA.CDS.8Carg07425-RA.CDS.8CDS
Carg07425-RA.CDS.9Carg07425-RA.CDS.9CDS
Carg07425-RA.CDS.10Carg07425-RA.CDS.10CDS


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg07425-RA.three_prime_UTR.1Carg07425-RA.three_prime_UTR.1three_prime_UTR


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3DG3DSA:3.90.230.10coord: 61..189
e-value: 8.2E-36
score: 125.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 31..46
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..82
NoneNo IPR availablePANTHERPTHR10804PROTEASE FAMILY M24 METHIONYL AMINOPEPTIDASE, AMINOPEPTIDASE Pcoord: 198..354
NoneNo IPR availablePANTHERPTHR10804:SF9METHIONINE AMINOPEPTIDASE 2coord: 32..186
coord: 198..354
NoneNo IPR availablePANTHERPTHR10804PROTEASE FAMILY M24 METHIONYL AMINOPEPTIDASE, AMINOPEPTIDASE Pcoord: 32..186
IPR036388Winged helix-like DNA-binding domain superfamilyGENE3DG3DSA:1.10.10.10coord: 248..326
e-value: 4.8E-40
score: 137.2
IPR000994Peptidase M24PFAMPF00557Peptidase_M24coord: 114..184
e-value: 3.1E-7
score: 30.2
coord: 198..341
e-value: 9.6E-7
score: 28.6
IPR002468Peptidase M24A, methionine aminopeptidase, subfamily 2CDDcd01088MetAP2coord: 112..350
e-value: 1.47286E-129
score: 374.664
IPR036005Creatinase/aminopeptidase-likeSUPERFAMILYSSF55920Creatinase/aminopeptidasecoord: 325..353
coord: 63..251
IPR036390Winged helix DNA-binding domain superfamilySUPERFAMILYSSF46785"Winged helix" DNA-binding domaincoord: 252..324