Carg06486-RA (mRNA) Silver-seed gourd

NameCarg06486-RA
TypemRNA
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionMethyltransferase-like protein 22
LocationCucurbita_argyrosperma_scaffold_051 : 788846 .. 793832 (-)
Sequence length1300
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTTCTTTTTTTGTCGCAAGGCGTGAATTTATCTCTCTTTCGGTTCCGGAACCGGCGAAGGACTTCCATGGACGACGGAATCTCAGAGGAGCTGGTGATGAGTGAGGTTCACCTTGGCTGCCCGCCGGGTTTTTCCGGGTCGTTTGTTTCCAATTTCACCATTTCCCTCCCTTCTGGTTCGTAATTCCATGCTTTCTCTGATTATCTCTTCCATTTTGGTATTGAACTTCAAAGCTGTTCCATTAGCTTGGCACTGCCTAGCACTTAACCATTTTCAGTTTTGTAATTAATGGCGGATGATAATAAGATTACTGACAAAATTATAGGTTTTGATGATTTAGATACAGAACCCAGAACCTATGTCAGTGATTCTGAAGGCTATTCACCACCGTCTACAAAGCAACTCATTCGTTTCGACGAAGATGGAGATCTTGTTTTACCCAGGCGCCTTAGTCAGTACCCAACACCTCTCTTCTAAGTTAATCATGAGTTTATAAGTTAGGAATACATATTCATTGATATGAGGCCTTTTGGGAAAACCAAAAGTAAAACTACGAGAGCTTATGCTCAAAGTAGACAATATCATACCATTGTAGAGAGTCGTGGTTTTTAACATGGTACTAAAGCCATGCCATTAACTTAGCCATGTCGATAGAATCCTCAAATGTCGAACAAAGAAGTTTTGAGCCTCGAAGGTGTAGTCAAAAGAAGTTTTTAGCCTTTGTTCGAGGGCTCCAGAGAAGGAGTCGAGCCTTAATTAAAAGAAGATTGTTTGAGGGCTTCATAGGCCTCATGGGAGGCTCTATGATGTGCTTTGTTCAAGGGGAGGATTGTTGAAGATTGTTGGAGAGGAGTCCCACGTCAGTTAATTAAGGGGTTGATCATGGATTTATAAGTAAGGAATACATGTCCATTGGTACAAGGCTTTTTAGAAACCAAAAGCAAAGCCATGAGAGCTTATGCTCAAATGAACAATATCATACCATTGTGGAGAGCTGTGATTTCTAACATACAAGTACCATATAGAGTACCCAAATTTCTTATGCTTTTTGCATTCTTGCATATAGTAATTTATGATTGCATTTTAAAGAGATCTGGAAAATTAAGTTAACTTTTTTAATGAACGCATAATTTCTTTCCTTTGAGTCAAAGTGTTCACAGTTGTTGTTAGGCAGTAATGCGAGCAGCTGATTATAAGCTATGTAACGCATTTCATCAAATGCCCGAACTGACCAAGAGGCTACACGATCACAATTACTTGCTGTGCTAGTAATTGATCTGTTATGAATGCAAATTTTACATCTCTCAGTTAAATCCCTTGCATTGTTCCCACTTGAAAATATTTAAATTGAAAATGTGAGTGTTAAAACACTCAATATCCCATCAGTTATTTAATTGCTGAAATGCTGTAACATTTCTATATGAAAACAGATGTGGAAGTAGAACCTTCAGTTCGTAGTTATAACGTGTCGATCCAACATGATATTATGTCAACGATCCCAAGTGTTGGTTTGCAGGTAATTGTTTCTTTCGATCTGTGATCTTATCCATTATTTTTTTTATTCATTTTATGATTCAGTGTCTTTCCTCTTTCAGGTGTGGAAAGCAGAACTAGTATTATCTGATTTTGTCTTGCATACAATGCTTACTTCGTCTAAATTCGATGGAATTGTTGCTCTAGAACTTGGAGCTGGAACAGGTGTTTGACGGACATGCATTCGTGATATTTATTTTTATGCACATTTGTTATTTTTCTGATTCCTACCATTTTGTATTAACGTACTGTTAGATGAAACAAATTTTCCTTTTTTTTCTTTTTTGAAAGAACCACCGTTGACTATATTACTATTTCAATGACAGGGTCTTATCTTGTTCCTCTTGCCAGGACTGGTTGGAATATTGCTTGCACGGGTTGCTAAAACAGTATTTCTAACAGGTATCGGCTATAGCAATCCTCAGAAATTTTGATTTTTTGTTATTCCTTGTTTTCCCATTTTGTAAATGAAACAATCTGATGATCTTGAGTTTCTCTTATAGTTTGATCTTGTTCTGTTCTGATGGATTGAGAAATGTGTGAGCGTGCACACTTTTCTCTTAACATGAAAACCAAAGTAAACTTAACAGTAACAGTCCAAGCCCACCGCTAGCCCACCGCTAACAGATATTATCCTCTTTGGGTTTAACCTTTCAGGCTTCCCCTCAAGGTTTTTAAAACGTGTCTGCTAGGGAGAGGTTTCCACACCCTTATAAAGAATGCTTCGTTCCCCTTCTCTAGCCGACGTGGGATCTCACAATTTATGTCTTTAAATATTAGTCAAATAAACCTGTAACTGCCTCTCATAGCTCACATATGTTTTAGTCGGATTAGTTGTGAGTAATAATTAGCTTAGAGGCTTATCTAACTTGTTTTTAACGTATGGATCCATTTCAAGTATTTTTAATGCGAAGGATGTGCTCTTAATGATTGACAGATAAAGGCGATCATATTCTTGACAACTGTGCCAAAAACATTGGTCTTAATTCTGGTGGATTCAGTGCTGGAGTTGCAGTTCATGTAAGGGAACTTGATTGGACAGAACCTTGGCCTCCAAAGCAAACCCGAGGAGAATGTCACCCAAATAACAGGTGATGAAAGCTTTGCAATTCACTATACCTGACAACTGTATGCCTTGGACTATGTTCATTTTTTCTCCCCATAAATGTGTGATTGGTATGTGACTTTAAATTGTTTAGCGATCACTGCAAGTGTTTCTTTTTTCACATTGGTAATATTTGTTTAAATTTTACATAGTGGTAAAAGTTGTTTTTGACCAAATAAAAAAATTTCTAAAACACTTCTTAATAACCCAAAGTGATTTGAGGAATTCTAAAAATAGATTAAAGTAAAACGGTTGAAATGATTTTAGATGAAACATGTAACAGCTCAAGCTCACCGCTAGCAGATAATGTCCTCTTTGAGCTTTCCTTTTCGAGATTCTCCTCAAAGTTTTTAAAATGCATATGTTAGGTAGAGATTCTACACCCTTCGGGGCCTAGCATCCTCGCTTACACTTGTTCCCTTCTCTAATCAATGTGGGACCTCCAATCCACTCCCTTCGAGGCGCAGCGTCCTTGCTAGCACTCATTCCCTTTCTCCAATCAATGTGGGACCCCTCAATCCACCCCCTACCCCTCAATCCACCCCTTCGGAGCCTAGCATCCTTGTGTTAAGTGTTCTTGGATGGAAGGGGACTTTTGAATTTCCTCGGGTGATTGCCATAAATCATCAATAAGTAAGTTTTTAAGTATATACTTCTTTCAAAAGGCTGCCGAACACACACTAAGCAATCTAAGACTTGTGCTCGTGGGTCCGGACTCATAGAACATAAAGTAACTGACGTTTCTTTTCTGGTGCCTTCATCGTCATACAGGTATTCCTGGGCCTTATCAGAGGTTGAAGAGGCACAGGGAGCCTGCTTACTTGTTGCTGCCGATGTTATCTATAGTGACGACTTGACAGATGCGTTTTTTAGTATCTTAGAGAAGTTTATGTCTCAAGGTTCAGAAAAGGTACGCATTCTCATGTTGAGATTATTATATTTTATTTGAGGTCTCCAATTGATGGGTTAGGTTAGTAGATAATGAAATATGCCTTTCATTTTATTTTTATATGGAAAAGATGCTAATCATTGACCATATGTAAAAGGACAAATGATATGAACAAACATTTGCTTGAAATAATGCATTATTAGGCAAGGGACATGAAATTTGTATATATATGTGGGGCCTTATATTCTACCACATTTCCTCGTTGATCCTCATTTGAGGAGACGGAGATGAAACCATTGGGGTTGACCCGAAATCACTTATTGATTCTTTGTAGCTAAGTTAGGCATCATGGAGTTGCAAGGAAACTTACTGCCAATCATTTACTCATCGTCAACCTGAGCATATTTCAACTAGTTAAGACGCATATTTCAACTAGTTAAGACATATTATTCTCAATCTAACCCACGTTGAACTCAAAACGAGACCTCTTACCTACTTTTCTTTGGAATTAATTACCTACTTTTCTTTGGAATTATGTAGCGAGCTCATGTATTGTCTTGGCTGAAACCATGAGGATGAGTAGCTATTGCGAAGTTTGGTTAATGTTTTGGTTGAACGTCAAATACATGTAATGTTAGATGAATTAATTATCACTTGTACCAAACCCACCAGGTGTTGTATTTGGCTTTGGAAAAGCGCTATAACTTCACTCTGAATGACTTTGACATTGTAGCAAATGGCTATTCTCATTTCCTAAGTTACCTGAAACATGAAGACGGTAAGTGACAGTGACATTCAGCACGTATCTCCGCCTTAAATGTTTCTAACTCGAGCTCATGTTCTACTTTTGGTACTCGACAGATTATTCCAAACACAGCAGATTGCAGCAGGAATCAAAACCATGTTTTATAGGCAACCGCATTGATCTCGCAAACATCCCACAATATGTCCTCAACTATGAAAGAGGGGAAGATGTAGAAATCTGGCAGATCAAGTATTGTAGGAAAGAGTCTTGAGATCAGAGAAGGGCCATGAACATCTGCTGTAGAACTCACAAATAGTTGCCTGCATTCATAACTCTGGTGAGTTTTCCCTTCAAAACTTCACCAAGAAAATGGGTATCAGTTATCACAGAACAAGAACTGAAGATATGAAATAATCTCAGTTCTAATTCTGTATTTTCTTCATTTCGTTTCACAATCTTCCTAATATTTTTTCGTCGTGAGGATTGATTTTGCCATTATAGATCTTTTGGAAAAATCTCAGGGTTATAATACTCAGTAATAATGGTATTTTTTTTTTTTAATATTTTTATTTGTAGGTAGAAGATTGAAGCATTGACCTTTGAATGATATGATGAATGATAGGGATGATAATTAGTTCATTTGTGGTATTGGCTTGAAGTATTATAAACAATGTTATGAGATATATGATGAGTAGCATTTGGCATTTAAAATTG

mRNA sequence

TTTTCTTTTTTTGTCGCAAGGCGTGAATTTATCTCTCTTTCGGTTCCGGAACCGGCGAAGGACTTCCATGGACGACGGAATCTCAGAGGAGCTGGTGATGAGTGAGGTTCACCTTGGCTGCCCGCCGGGTTTTTCCGGGTCGTTTGTTTCCAATTTCACCATTTCCCTCCCTTCTGGTTTTGATGATTTAGATACAGAACCCAGAACCTATGTCAGTGATTCTGAAGGCTATTCACCACCGTCTACAAAGCAACTCATTCGTTTCGACGAAGATGGAGATCTTGTTTTACCCAGGCGCCTTAATGTGGAAGTAGAACCTTCAGTTCGTAGTTATAACGTGTCGATCCAACATGATATTATGTCAACGATCCCAAGTGTTGGTTTGCAGGTGTGGAAAGCAGAACTAGTATTATCTGATTTTGTCTTGCATACAATGCTTACTTCGTCTAAATTCGATGGAATTGTTGCTCTAGAACTTGGAGCTGGAACAGGACTGGTTGGAATATTGCTTGCACGGGTTGCTAAAACAGTATTTCTAACAGATAAAGGCGATCATATTCTTGACAACTGTGCCAAAAACATTGGTCTTAATTCTGGTGGATTCAGTGCTGGAGTTGCAGTTCATGTAAGGGAACTTGATTGGACAGAACCTTGGCCTCCAAAGCAAACCCGAGGAGAATGTCACCCAAATAACAGGTATTCCTGGGCCTTATCAGAGGTTGAAGAGGCACAGGGAGCCTGCTTACTTGTTGCTGCCGATGTTATCTATAGTGACGACTTGACAGATGCGTTTTTTAGTATCTTAGAGAAGTTTATGTCTCAAGGTTCAGAAAAGGTGTTGTATTTGGCTTTGGAAAAGCGCTATAACTTCACTCTGAATGACTTTGACATTGTAGCAAATGGCTATTCTCATTTCCTAAGTTACCTGAAACATGAAGACGATTATTCCAAACACAGCAGATTGCAGCAGGAATCAAAACCATGTTTTATAGGCAACCGCATTGATCTCGCAAACATCCCACAATATGTCCTCAACTATGAAAGAGGGGAAGATGTAGAAATCTGGCAGATCAAGTATTGTAGGAAAGAGTCTTGAGATCAGAGAAGGGCCATGAACATCTGCTGTAGAACTCACAAATAGTTGCCTGCATTCATAACTCTGGTAGAAGATTGAAGCATTGACCTTTGAATGATATGATGAATGATAGGGATGATAATTAGTTCATTTGTGGTATTGGCTTGAAGTATTATAAACAATGTTATGAGATATATGATGAGTAGCATTTGGCATTTAAAATTG

Coding sequence (CDS)

ATGGACGACGGAATCTCAGAGGAGCTGGTGATGAGTGAGGTTCACCTTGGCTGCCCGCCGGGTTTTTCCGGGTCGTTTGTTTCCAATTTCACCATTTCCCTCCCTTCTGGTTTTGATGATTTAGATACAGAACCCAGAACCTATGTCAGTGATTCTGAAGGCTATTCACCACCGTCTACAAAGCAACTCATTCGTTTCGACGAAGATGGAGATCTTGTTTTACCCAGGCGCCTTAATGTGGAAGTAGAACCTTCAGTTCGTAGTTATAACGTGTCGATCCAACATGATATTATGTCAACGATCCCAAGTGTTGGTTTGCAGGTGTGGAAAGCAGAACTAGTATTATCTGATTTTGTCTTGCATACAATGCTTACTTCGTCTAAATTCGATGGAATTGTTGCTCTAGAACTTGGAGCTGGAACAGGACTGGTTGGAATATTGCTTGCACGGGTTGCTAAAACAGTATTTCTAACAGATAAAGGCGATCATATTCTTGACAACTGTGCCAAAAACATTGGTCTTAATTCTGGTGGATTCAGTGCTGGAGTTGCAGTTCATGTAAGGGAACTTGATTGGACAGAACCTTGGCCTCCAAAGCAAACCCGAGGAGAATGTCACCCAAATAACAGGTATTCCTGGGCCTTATCAGAGGTTGAAGAGGCACAGGGAGCCTGCTTACTTGTTGCTGCCGATGTTATCTATAGTGACGACTTGACAGATGCGTTTTTTAGTATCTTAGAGAAGTTTATGTCTCAAGGTTCAGAAAAGGTGTTGTATTTGGCTTTGGAAAAGCGCTATAACTTCACTCTGAATGACTTTGACATTGTAGCAAATGGCTATTCTCATTTCCTAAGTTACCTGAAACATGAAGACGATTATTCCAAACACAGCAGATTGCAGCAGGAATCAAAACCATGTTTTATAGGCAACCGCATTGATCTCGCAAACATCCCACAATATGTCCTCAACTATGAAAGAGGGGAAGATGTAGAAATCTGGCAGATCAAGTATTGTAGGAAAGAGTCTTGA

Protein sequence

MDDGISEELVMSEVHLGCPPGFSGSFVSNFTISLPSGFDDLDTEPRTYVSDSEGYSPPSTKQLIRFDEDGDLVLPRRLNVEVEPSVRSYNVSIQHDIMSTIPSVGLQVWKAELVLSDFVLHTMLTSSKFDGIVALELGAGTGLVGILLARVAKTVFLTDKGDHILDNCAKNIGLNSGGFSAGVAVHVRELDWTEPWPPKQTRGECHPNNRYSWALSEVEEAQGACLLVAADVIYSDDLTDAFFSILEKFMSQGSEKVLYLALEKRYNFTLNDFDIVANGYSHFLSYLKHEDDYSKHSRLQQESKPCFIGNRIDLANIPQYVLNYERGEDVEIWQIKYCRKES
BLAST of Carg06486-RA vs. NCBI nr
Match: XP_023528002.1 (methyltransferase-like protein 22 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 678.3 bits (1749), Expect = 1.3e-191
Identity = 336/342 (98.25%), Postives = 337/342 (98.54%), Query Frame = 0

Query: 1   MDDGISEELVMSEVHLGCPPGFSGSFVSNFTISLPSGFDDLDTEPRTYVSDSEGYSPPST 60
           MDDGISEELVMSEVHLGCPPGFSGSFVS+FTISLPSGFDDLDTEPRTYVSDSEGYS PST
Sbjct: 1   MDDGISEELVMSEVHLGCPPGFSGSFVSDFTISLPSGFDDLDTEPRTYVSDSEGYS-PST 60

Query: 61  KQLIRFDEDGDLVLPRRLNVEVEPSVRSYNVSIQHDIMSTIPSVGLQVWKAELVLSDFVL 120
           KQLI FDEDGDLVLPRRLNVEVEPSVRSYNVSIQHDIMSTIPSVGLQVWKAELVLSDFVL
Sbjct: 61  KQLIHFDEDGDLVLPRRLNVEVEPSVRSYNVSIQHDIMSTIPSVGLQVWKAELVLSDFVL 120

Query: 121 HTMLTSSKFDGIVALELGAGTGLVGILLARVAKTVFLTDKGDHILDNCAKNIGLNSGGFS 180
           HTMLTSSKFDGIVALELGAGTGLVGILLARVAKTVFLTDKGDHILDNCAKNIGLNSGGFS
Sbjct: 121 HTMLTSSKFDGIVALELGAGTGLVGILLARVAKTVFLTDKGDHILDNCAKNIGLNSGGFS 180

Query: 181 AGVAVHVRELDWTEPWPPKQTRGECHPNNRYSWALSEVEEAQGACLLVAADVIYSDDLTD 240
           AGVAVHVRELDWTEPWPPKQTRGECHPNNRYSWALSEVEEAQGACLLVAADVIYSDDLTD
Sbjct: 181 AGVAVHVRELDWTEPWPPKQTRGECHPNNRYSWALSEVEEAQGACLLVAADVIYSDDLTD 240

Query: 241 AFFSILEKFMSQGSEKVLYLALEKRYNFTLNDFDIVANGYSHFLSYLKHEDDYSKHSRLQ 300
           AFFSILEKFMSQGSEKVLYLALEKRYNFTLNDFDIVANGYSHFLSYLKHEDD  KHS LQ
Sbjct: 241 AFFSILEKFMSQGSEKVLYLALEKRYNFTLNDFDIVANGYSHFLSYLKHEDDDPKHSSLQ 300

Query: 301 QESKPCFIGNRIDLANIPQYVLNYERGEDVEIWQIKYCRKES 343
           QESKPCFIGNRIDLANIPQYVLNYERGEDVEIWQIKYCRKES
Sbjct: 301 QESKPCFIGNRIDLANIPQYVLNYERGEDVEIWQIKYCRKES 341

BLAST of Carg06486-RA vs. NCBI nr
Match: XP_022955571.1 (methyltransferase-like protein 22 isoform X1 [Cucurbita moschata])

HSP 1 Score: 675.6 bits (1742), Expect = 8.7e-191
Identity = 335/342 (97.95%), Postives = 336/342 (98.25%), Query Frame = 0

Query: 1   MDDGISEELVMSEVHLGCPPGFSGSFVSNFTISLPSGFDDLDTEPRTYVSDSEGYSPPST 60
           MDDGISEELVMSEVHLGCPPGFSGSFVSNFTISLPSGFDDLDTEPRTYVSDSEGYS PST
Sbjct: 1   MDDGISEELVMSEVHLGCPPGFSGSFVSNFTISLPSGFDDLDTEPRTYVSDSEGYS-PST 60

Query: 61  KQLIRFDEDGDLVLPRRLNVEVEPSVRSYNVSIQHDIMSTIPSVGLQVWKAELVLSDFVL 120
           +QLIRFDEDGDLVLPRRLNVEVEP V SYNVSIQHDIMSTIPSVGLQVWKAELVLSDFVL
Sbjct: 61  RQLIRFDEDGDLVLPRRLNVEVEPLVCSYNVSIQHDIMSTIPSVGLQVWKAELVLSDFVL 120

Query: 121 HTMLTSSKFDGIVALELGAGTGLVGILLARVAKTVFLTDKGDHILDNCAKNIGLNSGGFS 180
           HTMLTSSKFDGIVALELGAGTGLVGILLARVAKTVFLTDKGDHILDNCAKNIGLNSGGFS
Sbjct: 121 HTMLTSSKFDGIVALELGAGTGLVGILLARVAKTVFLTDKGDHILDNCAKNIGLNSGGFS 180

Query: 181 AGVAVHVRELDWTEPWPPKQTRGECHPNNRYSWALSEVEEAQGACLLVAADVIYSDDLTD 240
           AGVAVHVRELDWTEPWPPKQTRGECHPNNRYSWALSEVEEAQGACLLVAADVIYSDDLTD
Sbjct: 181 AGVAVHVRELDWTEPWPPKQTRGECHPNNRYSWALSEVEEAQGACLLVAADVIYSDDLTD 240

Query: 241 AFFSILEKFMSQGSEKVLYLALEKRYNFTLNDFDIVANGYSHFLSYLKHEDDYSKHSRLQ 300
           AFFSILEKFMSQGSEKVLYLALEKRYNFTLNDFDIVANGYSHFLSYLKHEDD  KHS LQ
Sbjct: 241 AFFSILEKFMSQGSEKVLYLALEKRYNFTLNDFDIVANGYSHFLSYLKHEDDDPKHSSLQ 300

Query: 301 QESKPCFIGNRIDLANIPQYVLNYERGEDVEIWQIKYCRKES 343
           QESKPCFIGNRIDLANIPQYVLNYERGEDVEIWQIKYCRKES
Sbjct: 301 QESKPCFIGNRIDLANIPQYVLNYERGEDVEIWQIKYCRKES 341

BLAST of Carg06486-RA vs. NCBI nr
Match: XP_022955572.1 (methyltransferase-like protein 22 isoform X2 [Cucurbita moschata])

HSP 1 Score: 666.8 bits (1719), Expect = 4.0e-188
Identity = 333/342 (97.37%), Postives = 334/342 (97.66%), Query Frame = 0

Query: 1   MDDGISEELVMSEVHLGCPPGFSGSFVSNFTISLPSGFDDLDTEPRTYVSDSEGYSPPST 60
           MDDGISEELVMSEVHLGCPPGFSGSFVSNFTISLPSGFDDLDTEPRTYVSDSEGYS PST
Sbjct: 1   MDDGISEELVMSEVHLGCPPGFSGSFVSNFTISLPSGFDDLDTEPRTYVSDSEGYS-PST 60

Query: 61  KQLIRFDEDGDLVLPRRLNVEVEPSVRSYNVSIQHDIMSTIPSVGLQVWKAELVLSDFVL 120
           +QLIRFDEDGDLVLPRRLNVEVEP V SYNVSIQHDIMSTIPSVGLQVWKAELVLSDFVL
Sbjct: 61  RQLIRFDEDGDLVLPRRLNVEVEPLVCSYNVSIQHDIMSTIPSVGLQVWKAELVLSDFVL 120

Query: 121 HTMLTSSKFDGIVALELGAGTGLVGILLARVAKTVFLTDKGDHILDNCAKNIGLNSGGFS 180
           HTMLTSSKFDGIVALELGAGTGLVGILLARVAKTVFLTDKGDHILDNCAKNIGLNSGGFS
Sbjct: 121 HTMLTSSKFDGIVALELGAGTGLVGILLARVAKTVFLTDKGDHILDNCAKNIGLNSGGFS 180

Query: 181 AGVAVHVRELDWTEPWPPKQTRGECHPNNRYSWALSEVEEAQGACLLVAADVIYSDDLTD 240
           AGVAVHVRELDWTEPWPPKQTRGECHPNNRYSWALSEVEEAQGACLLVAADVIYSDDLTD
Sbjct: 181 AGVAVHVRELDWTEPWPPKQTRGECHPNNRYSWALSEVEEAQGACLLVAADVIYSDDLTD 240

Query: 241 AFFSILEKFMSQGSEKVLYLALEKRYNFTLNDFDIVANGYSHFLSYLKHEDDYSKHSRLQ 300
           AFFSILEKFMSQGSEKVLYLALEKRYNFTLNDFDIVANGYSHFLSYLKHEDD    S LQ
Sbjct: 241 AFFSILEKFMSQGSEKVLYLALEKRYNFTLNDFDIVANGYSHFLSYLKHEDD----SSLQ 300

Query: 301 QESKPCFIGNRIDLANIPQYVLNYERGEDVEIWQIKYCRKES 343
           QESKPCFIGNRIDLANIPQYVLNYERGEDVEIWQIKYCRKES
Sbjct: 301 QESKPCFIGNRIDLANIPQYVLNYERGEDVEIWQIKYCRKES 337

BLAST of Carg06486-RA vs. NCBI nr
Match: XP_022980860.1 (methyltransferase-like protein 22 isoform X1 [Cucurbita maxima])

HSP 1 Score: 664.8 bits (1714), Expect = 1.5e-187
Identity = 332/342 (97.08%), Postives = 334/342 (97.66%), Query Frame = 0

Query: 1   MDDGISEELVMSEVHLGCPPGFSGSFVSNFTISLPSGFDDLDTEPRTYVSDSEGYSPPST 60
           MDDGISEELVMSEVHLGCPPGFSGSFVSNF+ISLPSGFDDLD EPRTYVSDSEGYS PST
Sbjct: 1   MDDGISEELVMSEVHLGCPPGFSGSFVSNFSISLPSGFDDLDIEPRTYVSDSEGYS-PST 60

Query: 61  KQLIRFDEDGDLVLPRRLNVEVEPSVRSYNVSIQHDIMSTIPSVGLQVWKAELVLSDFVL 120
           KQLIRFDEDGDLVLPRRLNVEVE SVRSYNVSIQHDIMSTIPSVGLQVWKAELVLSDFVL
Sbjct: 61  KQLIRFDEDGDLVLPRRLNVEVESSVRSYNVSIQHDIMSTIPSVGLQVWKAELVLSDFVL 120

Query: 121 HTMLTSSKFDGIVALELGAGTGLVGILLARVAKTVFLTDKGDHILDNCAKNIGLNSGGFS 180
           HTMLTSSKFDGIVALELGAGTGLVGILLARVAKTVFLTDKGDHILDNCAKNIGLNSGGFS
Sbjct: 121 HTMLTSSKFDGIVALELGAGTGLVGILLARVAKTVFLTDKGDHILDNCAKNIGLNSGGFS 180

Query: 181 AGVAVHVRELDWTEPWPPKQTRGECHPNNRYSWALSEVEEAQGACLLVAADVIYSDDLTD 240
           AGVAVHVRELDWTEPWPPKQTRGECHPNNRYSWALSEVEEAQGACLLVAADVIYSDDLTD
Sbjct: 181 AGVAVHVRELDWTEPWPPKQTRGECHPNNRYSWALSEVEEAQGACLLVAADVIYSDDLTD 240

Query: 241 AFFSILEKFMSQGSEKVLYLALEKRYNFTLNDFDIVANGYSHFLSYLKHEDDYSKHSRLQ 300
           AFFSILEKFMSQGSEKVLYLALEKRYNFTLNDFDIVANGYSHFLSYLKHE+D    S LQ
Sbjct: 241 AFFSILEKFMSQGSEKVLYLALEKRYNFTLNDFDIVANGYSHFLSYLKHEED----SSLQ 300

Query: 301 QESKPCFIGNRIDLANIPQYVLNYERGEDVEIWQIKYCRKES 343
           QESKPCFIGNRIDLANIPQYVLNYERGEDVEIWQIKYCRKES
Sbjct: 301 QESKPCFIGNRIDLANIPQYVLNYERGEDVEIWQIKYCRKES 337

BLAST of Carg06486-RA vs. NCBI nr
Match: XP_023528004.1 (methyltransferase-like protein 22 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 661.8 bits (1706), Expect = 1.3e-186
Identity = 331/342 (96.78%), Postives = 332/342 (97.08%), Query Frame = 0

Query: 1   MDDGISEELVMSEVHLGCPPGFSGSFVSNFTISLPSGFDDLDTEPRTYVSDSEGYSPPST 60
           MDDGISEELVMSEVHLGCPPGFSGSFVS+FTISLPS     DTEPRTYVSDSEGYS PST
Sbjct: 1   MDDGISEELVMSEVHLGCPPGFSGSFVSDFTISLPS-----DTEPRTYVSDSEGYS-PST 60

Query: 61  KQLIRFDEDGDLVLPRRLNVEVEPSVRSYNVSIQHDIMSTIPSVGLQVWKAELVLSDFVL 120
           KQLI FDEDGDLVLPRRLNVEVEPSVRSYNVSIQHDIMSTIPSVGLQVWKAELVLSDFVL
Sbjct: 61  KQLIHFDEDGDLVLPRRLNVEVEPSVRSYNVSIQHDIMSTIPSVGLQVWKAELVLSDFVL 120

Query: 121 HTMLTSSKFDGIVALELGAGTGLVGILLARVAKTVFLTDKGDHILDNCAKNIGLNSGGFS 180
           HTMLTSSKFDGIVALELGAGTGLVGILLARVAKTVFLTDKGDHILDNCAKNIGLNSGGFS
Sbjct: 121 HTMLTSSKFDGIVALELGAGTGLVGILLARVAKTVFLTDKGDHILDNCAKNIGLNSGGFS 180

Query: 181 AGVAVHVRELDWTEPWPPKQTRGECHPNNRYSWALSEVEEAQGACLLVAADVIYSDDLTD 240
           AGVAVHVRELDWTEPWPPKQTRGECHPNNRYSWALSEVEEAQGACLLVAADVIYSDDLTD
Sbjct: 181 AGVAVHVRELDWTEPWPPKQTRGECHPNNRYSWALSEVEEAQGACLLVAADVIYSDDLTD 240

Query: 241 AFFSILEKFMSQGSEKVLYLALEKRYNFTLNDFDIVANGYSHFLSYLKHEDDYSKHSRLQ 300
           AFFSILEKFMSQGSEKVLYLALEKRYNFTLNDFDIVANGYSHFLSYLKHEDD  KHS LQ
Sbjct: 241 AFFSILEKFMSQGSEKVLYLALEKRYNFTLNDFDIVANGYSHFLSYLKHEDDDPKHSSLQ 300

Query: 301 QESKPCFIGNRIDLANIPQYVLNYERGEDVEIWQIKYCRKES 343
           QESKPCFIGNRIDLANIPQYVLNYERGEDVEIWQIKYCRKES
Sbjct: 301 QESKPCFIGNRIDLANIPQYVLNYERGEDVEIWQIKYCRKES 336

BLAST of Carg06486-RA vs. TAIR10
Match: AT1G79915.1 (Putative methyltransferase family protein)

HSP 1 Score: 301.6 bits (771), Expect = 6.2e-82
Identity = 170/335 (50.75%), Postives = 211/335 (62.99%), Query Frame = 0

Query: 6   SEELVMSEVHLGCPPGFSGS-FVSNFTISLPSGFDDLDTEPRTYVSDSEGYSPPSTKQLI 65
           SE  VMSE+HLGCPP  +   F+S FT S PS                           +
Sbjct: 5   SEIEVMSEIHLGCPPAATDDPFLSRFTFSFPS-------------------------NTV 64

Query: 66  RFDEDGDLVLPRRLNVEVEPSVRSYNVSIQHDIMSTIPSVGLQVWKAELVLSDFVLHTML 125
             D+DGDL+LPRR      P+  S+ V+IQH I S +  VGLQVWKA+LVLS+FVLH + 
Sbjct: 65  SVDKDGDLLLPRR----NYPTGPSFTVTIQHSITSNLRHVGLQVWKAQLVLSEFVLHKIC 124

Query: 126 TSSKFDGIVALELGAGTGLVGILLARVAKTVFLTDKGDHILDNCAKNIGLNSGGFSAGVA 185
           TSS F GIV LELGAGTGL+GILLARVAK +FLTD GD IL NC KN+ LNS  F     
Sbjct: 125 TSSDFHGIVCLELGAGTGLLGILLARVAKAIFLTDHGDEILGNCGKNLELNSSLFHPHTV 184

Query: 186 VHVRELDWTEPWPPKQTRGECHP--NNRYSWALSEVEEAQGACLLVAADVIYSDDLTDAF 245
           V+VREL+W   WP + T     P   + Y W   + E  + +  + AADVIYSDDLT A 
Sbjct: 185 VNVRELNWMSDWPIQDTHA-LSPLFMSSYCWNKQDFELVKCSSFIFAADVIYSDDLTIAL 244

Query: 246 FSILEKFMSQGSEKVLYLALEKRYNFTLNDFDIVANGYSHFLSYLKHEDDYSKHSRLQQE 305
           FS+L + MS G +KVLYL LEKRYNF+L+D ++VANGY+ F SY+K ED   K      +
Sbjct: 245 FSMLRRVMSFGCDKVLYLGLEKRYNFSLDDLNVVANGYTCFRSYVK-EDGIGK------Q 302

Query: 306 SKPCFIGNRIDLANIPQYVLNYERGEDVEIWQIKY 338
            K  F+G RID+  IP Y+ +Y+RGEDVE+W+IKY
Sbjct: 305 EKRSFVGKRIDVTQIPHYLKDYDRGEDVELWEIKY 302

BLAST of Carg06486-RA vs. TAIR10
Match: AT2G26810.1 (Putative methyltransferase family protein)

HSP 1 Score: 47.0 bits (110), Expect = 2.7e-05
Identity = 25/90 (27.78%), Postives = 43/90 (47.78%), Query Frame = 0

Query: 105 GLQVWKAELVLSDFVLHTMLTSSKFDGIVALELGAGTGLVGILLARVAKTVFLTDKGDHI 164
           G  VW   ++++ ++      +    G   LELG+G G+ G+L ++  + V  TD  D +
Sbjct: 60  GQLVWPGAMLMNGYLSE---NADILQGCSVLELGSGVGITGVLCSKFCRKVIFTDHNDEV 119

Query: 165 LDNCAKNIGL--NSGGFSAGVAVHVRELDW 193
           L    KNI L  +S G      +   +L+W
Sbjct: 120 LKILKKNIDLHGHSSGPKPSAELEAAKLEW 146

BLAST of Carg06486-RA vs. TAIR10
Match: AT5G49560.1 (Putative methyltransferase family protein)

HSP 1 Score: 46.6 bits (109), Expect = 3.6e-05
Identity = 44/148 (29.73%), Postives = 65/148 (43.92%), Query Frame = 0

Query: 122 TMLTSSKFDGIVALELGAGTGLVGILLA-RVAKTVFLTDKGDHILDNCAKNIGLNSGGFS 181
           T+L+  K   +  LELG+GTGLVGI  A  ++  V +TD   H+LDN   N   N+    
Sbjct: 97  TLLSLKKPSPLNILELGSGTGLVGIAAAITLSANVTVTDL-PHVLDNLNFNAEANAEIVE 156

Query: 182 A-GVAVHVRELDWTEPWPPKQTRGECHPNNRYSWALSEVEE-AQGACLLVAADVIYSDDL 241
             G  V+V  L W E                      +VE   Q   L++A+DV+Y D L
Sbjct: 157 RFGGKVNVAPLRWGE--------------------ADDVEVLGQNVDLILASDVVYHDHL 216

Query: 242 TDAFFSILEKFMSQGSEKVLYLALEKRY 267
            +     L     +G   +  +A  +R+
Sbjct: 217 YEPLLKTLRLMQLEGKRLIFLMAHLRRW 223

BLAST of Carg06486-RA vs. TAIR10
Match: AT5G27400.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein)

HSP 1 Score: 45.1 bits (105), Expect = 1.0e-04
Identity = 43/133 (32.33%), Postives = 60/133 (45.11%), Query Frame = 0

Query: 105 GLQVWKAELVLSDFVLHTMLTSSKFDGIVALELGAGTGLVGILLARV-AKTVFLTDKGDH 164
           G  +W + L LS+FVL        F      E+G+G G+VGI LA V AK V LTD    
Sbjct: 148 GCSIWPSSLFLSEFVLS---FPELFANKACFEVGSGVGMVGICLAHVKAKEVILTDGDLL 207

Query: 165 ILDNCAKNIGLNSGGFSAGVAVHVRELDWTEPWPPKQTRGECHPNNRYSW-ALSEVEEAQ 224
            L N   N+  N         ++  +    +P   + TR +C       W   SE E +Q
Sbjct: 208 TLSNMKLNLERNH--------LNYDDEFLKQPGEAQSTRVKC---THLPWETASESELSQ 266

Query: 225 -GACLLVAADVIY 235
               +++ ADVIY
Sbjct: 268 YRPDIVLGADVIY 266

BLAST of Carg06486-RA vs. TAIR10
Match: AT1G08125.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein)

HSP 1 Score: 44.7 bits (104), Expect = 1.4e-04
Identity = 32/100 (32.00%), Postives = 51/100 (51.00%), Query Frame = 0

Query: 104 VGLQVWKAELVLSDFV----LHTMLTSSKFDGIVALELGAGTGLVGILLARVAKTVFLTD 163
           +G  VW A +V + ++         +SSK  G  A+ELGAG G+ G  LA +   V  TD
Sbjct: 41  LGTTVWDASMVFAKYLGKNSRKGRFSSSKLKGKRAIELGAGCGVAGFALAMLGCDVVTTD 100

Query: 164 KGD-------HILDNCAKNIGLNSGGFSAGVAVHVRELDW 193
           + +       ++  N ++ + +N G  SA  ++ V ELDW
Sbjct: 101 QKEVLPLLKRNVEWNTSRIVQMNPG--SAFGSLRVAELDW 138

BLAST of Carg06486-RA vs. Swiss-Prot
Match: sp|Q9BUU2|MET22_HUMAN (Methyltransferase-like protein 22 OS=Homo sapiens OX=9606 GN=METTL22 PE=1 SV=2)

HSP 1 Score: 136.7 bits (343), Expect = 4.7e-31
Identity = 116/347 (33.43%), Postives = 164/347 (47.26%), Query Frame = 0

Query: 17  GCPPGFSGSFVSNFTISLPSGFD---------------DLDT--EPRTYVSDSEGYSPPS 76
           G PP   GS   N   SL +G D               DLD    PR   SDS    P  
Sbjct: 86  GSPP---GSGHGNEGFSLQAGTDTTGQEVAEAQLDEDGDLDVVRRPRA-ASDSNPAGPLR 145

Query: 77  TK--QLIRFDEDGDLVLPRRLNVEVEPSVRSYNVSIQHDIMSTIPSVGLQVWKAELVLSD 136
            K   +I   E+ D+     L  E + S     + I+H + + +  VG QVW+  L+L+D
Sbjct: 146 DKVHPMILAQEEDDV-----LGEEAQGSPHDI-IRIEHTMATPLEDVGKQVWRGALLLAD 205

Query: 137 FVLHTMLTSSKFDGIVALELGAGTGLVGILLARVAKTVFLTDKGDHILDNCAKNIGLNSG 196
           ++L        F G  ALELGAGTGL  I+ A +A+TV+ TD G  +L  C +NI LNS 
Sbjct: 206 YIL---FRQDLFRGCTALELGAGTGLASIIAATMARTVYCTDVGADLLSMCQRNIALNSH 265

Query: 197 -GFSAGVAVHVRELDW------TEPWPPKQTRGECHPNNRYSWALSEVEEA-QGACLLVA 256
              + G  V V+ELDW      T+P  P            +SW+  E+ +      +L A
Sbjct: 266 LAATGGGIVRVKELDWLKDDLCTDPKVP------------FSWSQEEISDLYDHTTILFA 325

Query: 257 ADVIYSDDLTDAFFSILEKFMSQ-GSEKVLYLALEKRYNFTLNDFDIVANGYSHFLSYLK 316
           A+V Y DDLTDA F  L +   +  +     L++EKR NFTL   D+    Y HF S L 
Sbjct: 326 AEVFYDDDLTDAVFKTLSRLAHRLKNACTAILSVEKRLNFTLRHLDVTCEAYDHFRSCLH 385

Query: 317 HEDDYSKHSRLQQESKPCFIGNRIDLANIPQYVLNYERGEDVEIWQI 336
             +  +       + K  F+   ++ A+ PQ +L YER + +E+W+I
Sbjct: 386 ALEQLA-------DGKLRFVVEPVE-ASFPQ-LLVYERLQQLELWKI 398

BLAST of Carg06486-RA vs. Swiss-Prot
Match: sp|Q8R1C6|MET22_MOUSE (Methyltransferase-like protein 22 OS=Mus musculus OX=10090 GN=Mettl22 PE=2 SV=1)

HSP 1 Score: 134.0 bits (336), Expect = 3.1e-30
Identity = 109/350 (31.14%), Postives = 165/350 (47.14%), Query Frame = 0

Query: 19  PPGFSGSFVSNFTISLPSGFDDLDTEPRTYVSDSEGYSPPSTKQLIRFDEDGDLVLPRRL 78
           PP  SGS  S+  +SL +G D+         +  EG S P        DEDGDL + RR 
Sbjct: 73  PPAGSGSGHSHEDLSLQAGDDN---------TIQEGLSVP-------LDEDGDLDVVRRP 132

Query: 79  NV------------EVEPSVRSYN-----------------VSIQHDIMSTIPSVGLQVW 138
                         +V P++ +                   + I+H + + +  VG QVW
Sbjct: 133 RAASDPNPAEPARDKVHPTILAQEEDDLVGDQEYESCPHSIIKIEHTMATPLEDVGKQVW 192

Query: 139 KAELVLSDFVLHTMLTSSKFDGIVALELGAGTGLVGILLARVAKTVFLTDKGDHILDNCA 198
           +  L+L+D++L        F G   LELGAGTGL  I+ A +A TV+ TD G  +L  C 
Sbjct: 193 RGALLLADYIL---FRRDLFQGCTVLELGAGTGLASIVAATMAHTVYCTDVGTDLLAMCQ 252

Query: 199 KNIGLNSG-GFSAGVAVHVRELDWTEPWPPKQTRGEC-HPNNRYSWALSEVEEA-QGACL 258
           +N+ LNS    + G  V V+ELDW +          C  P   +SW+  E+ +      +
Sbjct: 253 RNVALNSHLTATGGGVVKVKELDWLKD-------NLCTDPKAPFSWSEEEIADLYDHTTV 312

Query: 259 LVAADVIYSDDLTDAFFSILEKFMSQ-GSEKVLYLALEKRYNFTLNDFDIVANGYSHFLS 318
           L+AA+V Y DDLT+A F+ L + + +  +      ++EKR+NFTL   D+    Y HF +
Sbjct: 313 LLAAEVFYDDDLTNALFNTLSRLVHRLKNACTAIFSVEKRFNFTLRHLDVTCEAYDHFRA 372

Query: 319 YLKHEDDYSKHSRLQQESKPCFIGNRIDLANIPQYVLNYERGEDVEIWQI 336
            L   +  +   RL+   +P         A+ PQ +L YER   +E+W+I
Sbjct: 373 SLDSLEKLA-DGRLRFMVEPV-------EASFPQ-LLVYERIRQLELWKI 387

BLAST of Carg06486-RA vs. Swiss-Prot
Match: sp|P38347|EFM2_YEAST (Protein-lysine N-methyltransferase EFM2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=EFM2 PE=1 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 1.3e-07
Identity = 59/200 (29.50%), Postives = 88/200 (44.00%), Query Frame = 0

Query: 86  VRSYNVSIQ-HDIMSTIPSVGLQVWKAELVLSDFV------LHT----MLTSSKFDGIVA 145
           +R+ + SI  H+   T  ++G + W + L+LS  V      LHT    ML +S    I  
Sbjct: 198 LRNLSTSIPLHEPSLTADNLGWKTWGSSLILSQLVVDHLDYLHTTNVNMLANSDIKQIKV 257

Query: 146 LELGAGTGLVGILLARVAK--------TVFLTDKGDHILDNCAKNIGLNSGGFSAGVAVH 205
           LELGAGTGLVG+  A   K         +F+TD  + I+ N  KN+ LN    + G  V 
Sbjct: 258 LELGAGTGLVGLSWALKWKELYGTENIEIFVTDLPE-IVTNLKKNVSLN----NLGDFVQ 317

Query: 206 VRELDWTEPWPPKQTRGECHPNNRYSWALSEVEEAQGACLLVAADVIYSDDLTDAFFSIL 265
              LDWT P       G     N +              +++ AD IYS    +   +++
Sbjct: 318 AEILDWTNPHDFIDKFGH---ENEFD-------------VILIADPIYSPQHPEWVVNMI 376

Query: 266 EKFMSQGSEKVLYLALEKRY 267
            KF++      L + L  +Y
Sbjct: 378 SKFLAASGTCHLEIPLRAKY 376

BLAST of Carg06486-RA vs. Swiss-Prot
Match: sp|Q86JB0|Y8324_DICDI (Putative uncharacterized protein DDB_G0277003 OS=Dictyostelium discoideum OX=44689 GN=DDB_G0277003 PE=4 SV=2)

HSP 1 Score: 51.6 bits (122), Expect = 2.0e-05
Identity = 52/190 (27.37%), Postives = 74/190 (38.95%), Query Frame = 0

Query: 104 VGLQVWKAELVLSDFVLHTMLTSSKFDGIVALELGAGTGLVGILL--ARVAKTVFLTDKG 163
           VG+  W A   LSDF+L      + F     LELG+GTGLVGI+L   +  K V LTD  
Sbjct: 144 VGMTTWGAAYQLSDFILS---NQNLFINKNILELGSGTGLVGIILDFIKPLKKVILTDYS 203

Query: 164 DHILDNCAKNIGLNSGGFSAGVAV------------------HVRELDWTEPWPPKQTRG 223
             +L N   N+ LN+      +                     VR LDW           
Sbjct: 204 PKVLKNLKFNMELNNLEIQDFINXXXXXXXXXXXXXXXXKINQVRVLDW----------- 263

Query: 224 ECHPNNRYSWALSEVEEAQGACLLVAADVIYSDDLTDAFFSILEKFMSQGSEKVLYLALE 274
           E    N     L+       + +++ AD++Y   L     SIL   + +    V Y++  
Sbjct: 264 EIEDLN----ILNNYSGLNDSNIILGADIVYEPSLCKYLVSILYFLLERNENSVAYISST 315

BLAST of Carg06486-RA vs. Swiss-Prot
Match: sp|O14118|EFM3_SCHPO (Protein-lysine N-methyltransferase efm3 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC3A11.03 PE=3 SV=3)

HSP 1 Score: 51.2 bits (121), Expect = 2.6e-05
Identity = 47/205 (22.93%), Postives = 89/205 (43.41%), Query Frame = 0

Query: 82  VEPSVRSYNVSIQHD--IMSTIPSVGLQVWKAELVLSDFVLHTMLTSSKFDGIVALELGA 141
           V+ S+  Y++ I+    ++    + G + W+A + L++++    + S    G+  LELGA
Sbjct: 96  VQYSLGEYDILIRESRHVLLREGTTGARTWEAGMALAEYIYQHPVQS----GMRVLELGA 155

Query: 142 GTGLVGILLARVAKTVFLTDKGDHILDNCAKNIGLNSGGFSAGVAVHVRELDWTEPWPPK 201
           GTGLV IL A++   V  TD    + D   +N  LN+        ++V++L W       
Sbjct: 156 GTGLVSILCAKMGSIVLATDGDTKVCDGVRENARLNN------CDINVKKLLW------- 215

Query: 202 QTRGECHPNNRYSWALSEVEEAQGACLLVAADVIYSDDLTDAFFSILEKFMSQGSEKVLY 261
                             V+  + + ++ A+DV Y  DL     ++ +      + K++ 
Sbjct: 216 -----------------GVDPPEFSDIVFASDVTYDCDLRCLATTLTQIITINPNCKIIL 266

Query: 262 LALEKRYNFTLNDFDIVANGYSHFL 285
            A  +R     N   ++ N Y+  L
Sbjct: 276 SASLRRQETFFNFLKLIQNLYARQL 266

BLAST of Carg06486-RA vs. TrEMBL
Match: tr|A0A1S3BHK9|A0A1S3BHK9_CUCME (methyltransferase-like protein 22 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103489932 PE=4 SV=1)

HSP 1 Score: 599.7 bits (1545), Expect = 4.0e-168
Identity = 298/342 (87.13%), Postives = 320/342 (93.57%), Query Frame = 0

Query: 1   MDDGISEELVMSEVHLGCPPGFSGSFVSNFTISLPSGFDDLDTEPRTYVSDSEGYSPPST 60
           MDDGISEELVMSEVHLGCPPGF GS+VSNFTIS PSG DDLDTE +TYVSDS      S+
Sbjct: 23  MDDGISEELVMSEVHLGCPPGFLGSYVSNFTISFPSGSDDLDTETKTYVSDSS-----SS 82

Query: 61  KQLIRFDEDGDLVLPRRLNVEVEPSVRSYNVSIQHDIMSTIPSVGLQVWKAELVLSDFVL 120
           KQLI FDEDGDLVLPRR+NVE EPSVRS+NVSIQHD+MSTIPSVGLQVWKAELVLSDFVL
Sbjct: 83  KQLIGFDEDGDLVLPRRVNVE-EPSVRSFNVSIQHDVMSTIPSVGLQVWKAELVLSDFVL 142

Query: 121 HTMLTSSKFDGIVALELGAGTGLVGILLARVAKTVFLTDKGDHILDNCAKNIGLNSGGFS 180
           HTMLTSS+F GIVALELGAGTGLVGILLARVAKT+FLTDKGD +LDNCAKNI LNSGGFS
Sbjct: 143 HTMLTSSEFQGIVALELGAGTGLVGILLARVAKTIFLTDKGDRVLDNCAKNINLNSGGFS 202

Query: 181 AGVAVHVRELDWTEPWPPKQTRGECHPNNRYSWALSEVEEAQGACLLVAADVIYSDDLTD 240
           AGVAVHVRELDWT+PWPPK+T+GEC PNNRYSWA SEVEEA GA LLVAADVIYSDDLTD
Sbjct: 203 AGVAVHVRELDWTKPWPPKRTQGECRPNNRYSWASSEVEEALGASLLVAADVIYSDDLTD 262

Query: 241 AFFSILEKFMSQGSEKVLYLALEKRYNFTLNDFDIVANGYSHFLSYLKHEDDYSKHSRLQ 300
           AFF++LEKFMSQGSEKVLYLALEKRYNFTL+DFDIVANGYSHFLSYLKHE D +++SRL+
Sbjct: 263 AFFNMLEKFMSQGSEKVLYLALEKRYNFTLDDFDIVANGYSHFLSYLKHEKDDTENSRLE 322

Query: 301 QESKPCFIGNRIDLANIPQYVLNYERGEDVEIWQIKYCRKES 343
           QESKP F+G+RIDLANIPQYVLNYERG+DVEIWQIKYCRKES
Sbjct: 323 QESKPYFVGHRIDLANIPQYVLNYERGKDVEIWQIKYCRKES 358

BLAST of Carg06486-RA vs. TrEMBL
Match: tr|A0A1S3BHK0|A0A1S3BHK0_CUCME (methyltransferase-like protein 22 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103489932 PE=4 SV=1)

HSP 1 Score: 525.4 bits (1352), Expect = 9.6e-146
Identity = 267/342 (78.07%), Postives = 286/342 (83.63%), Query Frame = 0

Query: 1   MDDGISEELVMSEVHLGCPPGFSGSFVSNFTISLPSGFDDLDTEPRTYVSDSEGYSPPST 60
           MDDGISEELVMSEVHLGCPPGF GS+VSNFTIS PS     D E                
Sbjct: 23  MDDGISEELVMSEVHLGCPPGFLGSYVSNFTISFPS-----DVE---------------- 82

Query: 61  KQLIRFDEDGDLVLPRRLNVEVEPSVRSYNVSIQHDIMSTIPSVGLQVWKAELVLSDFVL 120
                                 EPSVRS+NVSIQHD+MSTIPSVGLQVWKAELVLSDFVL
Sbjct: 83  ----------------------EPSVRSFNVSIQHDVMSTIPSVGLQVWKAELVLSDFVL 142

Query: 121 HTMLTSSKFDGIVALELGAGTGLVGILLARVAKTVFLTDKGDHILDNCAKNIGLNSGGFS 180
           HTMLTSS+F GIVALELGAGTGLVGILLARVAKT+FLTDKGD +LDNCAKNI LNSGGFS
Sbjct: 143 HTMLTSSEFQGIVALELGAGTGLVGILLARVAKTIFLTDKGDRVLDNCAKNINLNSGGFS 202

Query: 181 AGVAVHVRELDWTEPWPPKQTRGECHPNNRYSWALSEVEEAQGACLLVAADVIYSDDLTD 240
           AGVAVHVRELDWT+PWPPK+T+GEC PNNRYSWA SEVEEA GA LLVAADVIYSDDLTD
Sbjct: 203 AGVAVHVRELDWTKPWPPKRTQGECRPNNRYSWASSEVEEALGASLLVAADVIYSDDLTD 262

Query: 241 AFFSILEKFMSQGSEKVLYLALEKRYNFTLNDFDIVANGYSHFLSYLKHEDDYSKHSRLQ 300
           AFF++LEKFMSQGSEKVLYLALEKRYNFTL+DFDIVANGYSHFLSYLKHE D +++SRL+
Sbjct: 263 AFFNMLEKFMSQGSEKVLYLALEKRYNFTLDDFDIVANGYSHFLSYLKHEKDDTENSRLE 321

Query: 301 QESKPCFIGNRIDLANIPQYVLNYERGEDVEIWQIKYCRKES 343
           QESKP F+G+RIDLANIPQYVLNYERG+DVEIWQIKYCRKES
Sbjct: 323 QESKPYFVGHRIDLANIPQYVLNYERGKDVEIWQIKYCRKES 321

BLAST of Carg06486-RA vs. TrEMBL
Match: tr|M5X0H5|M5X0H5_PRUPE (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_ppa015023mg PE=4 SV=1)

HSP 1 Score: 411.0 bits (1055), Expect = 2.6e-111
Identity = 211/335 (62.99%), Postives = 250/335 (74.63%), Query Frame = 0

Query: 3   DGISEELVMSEVHLGCPPGFSGSFVSNFTISLPSGFDDLDTEPRTYVSDSEGYSPPSTKQ 62
           D   EE VMSEVHLGCP GFSG  +S FT S+P+     + EPR Y   S+   P ST Q
Sbjct: 8   DSQEEEQVMSEVHLGCPSGFSGPHISRFTFSIPT-----ELEPRRYNGTSQ-VEPASTNQ 67

Query: 63  LIRFDEDGDLVLPRRLNVEVEPSVRSYNVSIQHDIMSTIPSVGLQVWKAELVLSDFVLHT 122
           +I  DEDGDL+LPRR  +    S +S +VSIQH+I S+IPSVGLQVW+AEL+LSDF+LH 
Sbjct: 68  MISLDEDGDLLLPRRNKL----STQSSSVSIQHNITSSIPSVGLQVWRAELLLSDFLLHK 127

Query: 123 MLTSSKFDGIVALELGAGTGLVGILLARVAKTVFLTDKGDHILDNCAKNIGLNSGGFSAG 182
           M TSS+ DG VALELGAGTGLVG+LLARVAKT+F+TD G+ +LDNCAKN+ LNS  FS  
Sbjct: 128 MFTSSELDGTVALELGAGTGLVGVLLARVAKTIFITDHGNEVLDNCAKNVDLNSELFSHR 187

Query: 183 VAVHVRELDWTEPWPPKQTRGECHPNNRYSWALSEVEEAQGACLLVAADVIYSDDLTDAF 242
             VHVRELDW +PWPP+ T  E     RYSW  SEVE AQ A LLVAADVIYSDDLTDAF
Sbjct: 188 ATVHVRELDWMKPWPPRMTLEESSLGKRYSWTFSEVEAAQEASLLVAADVIYSDDLTDAF 247

Query: 243 FSILEKFMSQGSEKVLYLALEKRYNFTLNDFDIVANGYSHFLSYLKHEDDYSKHSRLQQE 302
           FS L++ MS GSEKVLYLALEKRYNF+++D ++VANGYSHF S+L+ E D      L+  
Sbjct: 248 FSTLQRLMSSGSEKVLYLALEKRYNFSVDDLNVVANGYSHFRSFLRLEGD---SEALECG 307

Query: 303 SKPCFIGNRIDLANIPQYVLNYERGEDVEIWQIKY 338
           S PCF+G  ID+  IPQYV  Y+RG DVE+WQI+Y
Sbjct: 308 SMPCFVGKCIDITQIPQYVREYDRGTDVELWQIEY 329

BLAST of Carg06486-RA vs. TrEMBL
Match: tr|A0A2N9FRG1|A0A2N9FRG1_FAGSY (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS21189 PE=4 SV=1)

HSP 1 Score: 409.8 bits (1052), Expect = 5.9e-111
Identity = 212/338 (62.72%), Postives = 250/338 (73.96%), Query Frame = 0

Query: 3   DGISEELVMSEVHLGCPPGFSGSFVSNFTISLPSGFDDLDTEPRTYVSDSEGYSPPSTKQ 62
           D   EE VMSEVHLGCPPG SGS +S+FTIS+P+     + EP  Y  +S          
Sbjct: 5   DSAEEEEVMSEVHLGCPPGLSGSHISHFTISVPA-XXXXEVEPSRYECES---------- 64

Query: 63  LIRFDEDGDLVLPRRLNVEVEPSVRSYNVSIQHDIMSTIPSVGLQVWKAELVLSDFVLHT 122
             R D+DGDL+L RR     +P+   Y V+IQH+I S+IP+VGLQVWKAELVLSDFVLH 
Sbjct: 65  -FRMDQDGDLLLTRR----HKPASSIYRVTIQHNITSSIPNVGLQVWKAELVLSDFVLHK 124

Query: 123 MLTSSKFDGIVALELGAGTGLVGILLARVAKTVFLTDKGDHILDNCAKNIGLNSGGFSAG 182
           M TSS F GIVALELGAGTGLVGILLARVAKTVF+TD GD IL+NCA N+ LNS  F+  
Sbjct: 125 MFTSSDFHGIVALELGAGTGLVGILLARVAKTVFVTDHGDEILNNCATNVELNSALFNCQ 184

Query: 183 VAVHVRELDWTEPWPPKQTRGECHPNNRYSWALSEVEEAQGACLLVAADVIYSDDLTDAF 242
            AVHVREL+W  PWPP+   GE   + RYSW  SEV++ QGA LLVAADVIYSDDLTDAF
Sbjct: 185 AAVHVRELEWMSPWPPRVGPGESPLHKRYSWTFSEVDQVQGASLLVAADVIYSDDLTDAF 244

Query: 243 FSILEKFMSQGSEKVLYLALEKRYNFTLNDFDIVANGYSHFLSYLKHEDDYSKHSRLQQE 302
           FS+LE+ MS GSEKVLYLALEKRYNF++NDF++VANGY HF SYL+ + D      ++  
Sbjct: 245 FSVLERLMSLGSEKVLYLALEKRYNFSVNDFNVVANGYLHFQSYLRDDKD---SEDIKNG 304

Query: 303 SKPCFIGNRIDLANIPQYVLNYERGEDVEIWQIKYCRK 341
           S PCF G RID+ +IP YV +Y+RG DVEIWQIKY +K
Sbjct: 305 SAPCFAGKRIDITHIPHYVRDYDRGNDVEIWQIKYEKK 323

BLAST of Carg06486-RA vs. TrEMBL
Match: tr|A0A2P4HQB1|A0A2P4HQB1_QUESU (Methyltransferase-like protein 22 OS=Quercus suber OX=58331 GN=CFP56_08945 PE=4 SV=1)

HSP 1 Score: 409.8 bits (1052), Expect = 5.9e-111
Identity = 214/341 (62.76%), Postives = 256/341 (75.07%), Query Frame = 0

Query: 2   DDGISEELVMSEVHLGCPPGFSGSFVSNFTISLPSGFDDLDTEPRTYVSDSEGYSPPSTK 61
           D  + EE VMSEVHLGCPPG SG  +S+FTISLP+     + EP  Y  ++      S  
Sbjct: 30  DSTMEEEHVMSEVHLGCPPGISGPHISHFTISLPT---PPEVEPSRYQCEA-----ASPN 89

Query: 62  QLIRFDEDGDLVLPRRLNVEVEPSVRSYNVSIQHDIMSTIPSVGLQVWKAELVLSDFVLH 121
           Q    D DGDLVL RR     +P+   Y V+IQH+I S+IP+VGLQVW+AELVLSDFVLH
Sbjct: 90  QTFSVDPDGDLVLTRR----NKPASSIYRVTIQHNITSSIPNVGLQVWRAELVLSDFVLH 149

Query: 122 TMLTSSKFDGIVALELGAGTGLVGILLARVAKTVFLTDKGDHILDNCAKNIGLNSGGFSA 181
            M TS++ DGIVALELGAGTGLVGILLA VAKTVF+TD GD IL+NCA+N+ LNSG F+ 
Sbjct: 150 KMFTSTELDGIVALELGAGTGLVGILLAHVAKTVFVTDHGDEILNNCAENVDLNSGLFNC 209

Query: 182 GVAVHVRELDWTEPWPPKQT-RGECHPNNRYSWALSEVEEAQGACLLVAADVIYSDDLTD 241
             AV+VREL+W  PWPP+    GE   + RYSW  SEVEE QGA LLVAADVIYSDDLTD
Sbjct: 210 QAAVYVRELEWMSPWPPRVVGPGESPLHKRYSWTFSEVEEVQGASLLVAADVIYSDDLTD 269

Query: 242 AFFSILEKFMSQGSEKVLYLALEKRYNFTLNDFDIVANGYSHFLSYLKHEDDYSKHSRLQ 301
           AFF++LEKFMS GS+KVLYLALEKRYNF++ND D+VANGYSHF SYL+   D S+   ++
Sbjct: 270 AFFNVLEKFMSLGSKKVLYLALEKRYNFSVNDLDVVANGYSHFRSYLR---DDSECEDIK 329

Query: 302 QESKPCFIGNRIDLANIPQYVLNYERGEDVEIWQIKYCRKE 342
             S PCF+G RID+  IPQYV +Y+RG DVEIW+IKY +K+
Sbjct: 330 NGSVPCFVGKRIDITQIPQYVRDYDRGNDVEIWRIKYEKKK 355

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023528002.11.3e-19198.25methyltransferase-like protein 22 isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_022955571.18.7e-19197.95methyltransferase-like protein 22 isoform X1 [Cucurbita moschata][more]
XP_022955572.14.0e-18897.37methyltransferase-like protein 22 isoform X2 [Cucurbita moschata][more]
XP_022980860.11.5e-18797.08methyltransferase-like protein 22 isoform X1 [Cucurbita maxima][more]
XP_023528004.11.3e-18696.78methyltransferase-like protein 22 isoform X2 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT1G79915.16.2e-8250.75Putative methyltransferase family protein[more]
AT2G26810.12.7e-0527.78Putative methyltransferase family protein[more]
AT5G49560.13.6e-0529.73Putative methyltransferase family protein[more]
AT5G27400.11.0e-0432.33S-adenosyl-L-methionine-dependent methyltransferases superfamily protein[more]
AT1G08125.21.4e-0432.00S-adenosyl-L-methionine-dependent methyltransferases superfamily protein[more]
Match NameE-valueIdentityDescription
sp|Q9BUU2|MET22_HUMAN4.7e-3133.43Methyltransferase-like protein 22 OS=Homo sapiens OX=9606 GN=METTL22 PE=1 SV=2[more]
sp|Q8R1C6|MET22_MOUSE3.1e-3031.14Methyltransferase-like protein 22 OS=Mus musculus OX=10090 GN=Mettl22 PE=2 SV=1[more]
sp|P38347|EFM2_YEAST1.3e-0729.50Protein-lysine N-methyltransferase EFM2 OS=Saccharomyces cerevisiae (strain ATCC... [more]
sp|Q86JB0|Y8324_DICDI2.0e-0527.37Putative uncharacterized protein DDB_G0277003 OS=Dictyostelium discoideum OX=446... [more]
sp|O14118|EFM3_SCHPO2.6e-0522.93Protein-lysine N-methyltransferase efm3 OS=Schizosaccharomyces pombe (strain 972... [more]
Match NameE-valueIdentityDescription
tr|A0A1S3BHK9|A0A1S3BHK9_CUCME4.0e-16887.13methyltransferase-like protein 22 isoform X1 OS=Cucumis melo OX=3656 GN=LOC10348... [more]
tr|A0A1S3BHK0|A0A1S3BHK0_CUCME9.6e-14678.07methyltransferase-like protein 22 isoform X2 OS=Cucumis melo OX=3656 GN=LOC10348... [more]
tr|M5X0H5|M5X0H5_PRUPE2.6e-11162.99Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_ppa015023mg PE=4 SV=1[more]
tr|A0A2N9FRG1|A0A2N9FRG1_FAGSY5.9e-11162.72Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS21189 PE=4 SV=1[more]
tr|A0A2P4HQB1|A0A2P4HQB1_QUESU5.9e-11162.76Methyltransferase-like protein 22 OS=Quercus suber OX=58331 GN=CFP56_08945 PE=4 ... [more]
The following terms have been associated with this mRNA:
Vocabulary: Molecular Function
TermDefinition
GO:0008276protein methyltransferase activity
Vocabulary: Biological Process
TermDefinition
GO:0006479protein methylation
Vocabulary: INTERPRO
TermDefinition
IPR029063SAM-dependent_MTases
IPR038899METTL22
IPR019410Methyltransf_16
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006479 protein methylation
cellular_component GO:0005575 cellular_component
molecular_function GO:0008276 protein methyltransferase activity

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Carg06486Carg06486gene


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg06486-RA.three_prime_UTR.2Carg06486-RA.three_prime_UTR.2three_prime_UTR
Carg06486-RA.three_prime_UTR.1Carg06486-RA.three_prime_UTR.1three_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg06486-RA.CDS.9Carg06486-RA.CDS.9CDS
Carg06486-RA.CDS.8Carg06486-RA.CDS.8CDS
Carg06486-RA.CDS.7Carg06486-RA.CDS.7CDS
Carg06486-RA.CDS.6Carg06486-RA.CDS.6CDS
Carg06486-RA.CDS.5Carg06486-RA.CDS.5CDS
Carg06486-RA.CDS.4Carg06486-RA.CDS.4CDS
Carg06486-RA.CDS.3Carg06486-RA.CDS.3CDS
Carg06486-RA.CDS.2Carg06486-RA.CDS.2CDS
Carg06486-RA.CDS.1Carg06486-RA.CDS.1CDS


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg06486-RA.five_prime_UTR.1Carg06486-RA.five_prime_UTR.1five_prime_UTR


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg06486-RA.exon.10Carg06486-RA.exon.10exon
Carg06486-RA.exon.9Carg06486-RA.exon.9exon
Carg06486-RA.exon.8Carg06486-RA.exon.8exon
Carg06486-RA.exon.7Carg06486-RA.exon.7exon
Carg06486-RA.exon.6Carg06486-RA.exon.6exon
Carg06486-RA.exon.5Carg06486-RA.exon.5exon
Carg06486-RA.exon.4Carg06486-RA.exon.4exon
Carg06486-RA.exon.3Carg06486-RA.exon.3exon
Carg06486-RA.exon.2Carg06486-RA.exon.2exon
Carg06486-RA.exon.1Carg06486-RA.exon.1exon


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Carg06486-RACarg06486-RA-proteinpolypeptide


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR019410Lysine methyltransferasePFAMPF10294Methyltransf_16coord: 101..200
e-value: 1.6E-16
score: 60.4
NoneNo IPR availableGENE3DG3DSA:3.40.50.150coord: 80..292
e-value: 1.3E-36
score: 128.0
NoneNo IPR availablePANTHERPTHR23108:SF0METHYLTRANSFERASE-LIKE PROTEIN 22coord: 5..339
IPR038899Methyltransferase-like protein 22PANTHERPTHR23108METHYLTRANSFERASE-RELATEDcoord: 5..339
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILYSSF53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 124..203