Carg05185-RA (mRNA) Silver-seed gourd

BLAST of Carg05185-RA vs. NCBI nr
Match: XP_022947462.1 (probable ubiquitin-conjugating enzyme E2 23 [Cucurbita moschata])

HSP 1 Score: 2132.1 bits (5523), Expect = 0.0e+00
Identity = 1141/1147 (99.48%), Postives = 1141/1147 (99.48%), Query Frame = 0

Query: 15   MGQQDSVDEESLTDTCDSYMNRDGVSTSGNIHMPTINSDEARRMNENRSDKHNIPHVYRR 74
            MGQQDSVDEESLTD CDSYMNRDGVSTSGNIHMPTINSDEARRMNENRSDKHNIPHVYRR
Sbjct: 1    MGQQDSVDEESLTDACDSYMNRDGVSTSGNIHMPTINSDEARRMNENRSDKHNIPHVYRR 60

Query: 75   DIVKSKGSGMIGIVTEVAGDADSDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 134
            DIVKSKGSGMIGIVTEVAGDADSDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 61   DIVKSKGSGMIGIVTEVAGDADSDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 120

Query: 135  XXXXXXXXXXXXXXXXXXXQPLPADEVRVLWMDESETTQNVNDLTVIDRGFLHGDFVAAV 194
            XXXXXXXXXXXXXXXXXXXQPLPADEVRVLWMDESETTQNVNDLTV DRGFLHGDFVAAV
Sbjct: 121  XXXXXXXXXXXXXXXXXXXQPLPADEVRVLWMDESETTQNVNDLTVTDRGFLHGDFVAAV 180

Query: 195  SNPTGQAGVVVDVNISVDLLVPDGSVVKNISSKDLIRVRDFAVGDYVVLGPWLGRIDDVL 254
            SNPTGQAGVVVDVNISVDLLVPDGSVVKNISSKDLIRVRDFAVGDYVVLGPWLGRIDDVL
Sbjct: 181  SNPTGQAGVVVDVNISVDLLVPDGSVVKNISSKDLIRVRDFAVGDYVVLGPWLGRIDDVL 240

Query: 255  DNVTVMFDDGSKCKVTKAEPSRLKPVSKNILEDASFPYYPGQRVRASSSTVFKNSKWLSG 314
            DNVTVMFDDGSKCKVTKAEPSRLKPVSKNILEDASFPYYPGQRVRASSSTVFKNSKWLSG
Sbjct: 241  DNVTVMFDDGSKCKVTKAEPSRLKPVSKNILEDASFPYYPGQRVRASSSTVFKNSKWLSG 300

Query: 315  LWKPNRLQGTVTKVTVGSVFIYWIASAGYGPDSSTTPAEEQSPKNLRLLTCFSHVNWQLG 374
            LWKPNRLQGTVTKVTVGSVFIYWIASAGYGPDSSTTPAEEQSPKNLRLLTCFSHVNWQLG
Sbjct: 301  LWKPNRLQGTVTKVTVGSVFIYWIASAGYGPDSSTTPAEEQSPKNLRLLTCFSHVNWQLG 360

Query: 375  DWCLLPSSFSSGSTKDLSQTELSDSVTNTLDYAQTVGACDSEDTVVDELSGRTESTDLDS 434
            DWCLLPSSFSSGSTKDLSQTELSDSVTNTLDYAQTVGACDSEDTVVDELSGRTESTDLDS
Sbjct: 361  DWCLLPSSFSSGSTKDLSQTELSDSVTNTLDYAQTVGACDSEDTVVDELSGRTESTDLDS 420

Query: 435  ISACNGNYRDPECNILPESSSSRATKENTHETWPLHRKKIRKVVIRRDKXXXXXXXNFER 494
            ISACNGNYRDPECNILPESSSSRATKENTHETWPLHRKKIRKVVIRRDKXXXXXXXNFER
Sbjct: 421  ISACNGNYRDPECNILPESSSSRATKENTHETWPLHRKKIRKVVIRRDKXXXXXXXNFER 480

Query: 495  ALLIINTTTKVDVVWQDGRTELALDSTGLIPINPGDHEFVPEQYVVEKATDNDDDVTESR 554
            ALLIINTTTKVDVVWQDGRTELALDSTGLIPINPGDHEFVPEQYVVEKATDNDDDVTESR
Sbjct: 481  ALLIINTTTKVDVVWQDGRTELALDSTGLIPINPGDHEFVPEQYVVEKATDNDDDVTESR 540

Query: 555  RVGVVKSVHAKERTACVRWLKPVSRAEDPREFDIEEVVSVYELEGHPDYDYCYGDVVVRL 614
            RVGVVKSVHAKERTACVRWLKPVSRAEDPREFDIEEVVSVYELEGHPDYDYCYGDVVVRL
Sbjct: 541  RVGVVKSVHAKERTACVRWLKPVSRAEDPREFDIEEVVSVYELEGHPDYDYCYGDVVVRL 600

Query: 615  SPVSDSIEALSSENNTEELNQQSSTNGVMSCPEKVSGSLKIEYPSSSDDCFDFSDLSWVG 674
            SPVSDSIEALSSENNTEELNQQSSTNGVMSCPEKVSGSLKIEYPSSSDDCFDFSDLSWVG
Sbjct: 601  SPVSDSIEALSSENNTEELNQQSSTNGVMSCPEKVSGSLKIEYPSSSDDCFDFSDLSWVG 660

Query: 675  NITNLKNGDIEVFWANGMVSTVGPQAIYVVGRDDDDESTGAGSEISNGAASWETVXXXXX 734
            NITNLKNGDIEVFWANGMVSTVGPQAIYVVGRDDDDES GAGSEISNGAASWETV  XXX
Sbjct: 661  NITNLKNGDIEVFWANGMVSTVGPQAIYVVGRDDDDESIGAGSEISNGAASWETVDDXXX 720

Query: 735  XMDVENAKEDIELQDTGVDSEEEESEQHNSGRSLALSVPLAAFRFVTRLATGIFSRGPRN 794
             MDVENAKEDIELQDTGVDSEEEESEQHNSGRSLALSVPLAAFRFVTRLATGIFSRGPRN
Sbjct: 721  EMDVENAKEDIELQDTGVDSEEEESEQHNSGRSLALSVPLAAFRFVTRLATGIFSRGPRN 780

Query: 795  RESMDLDSHSESDTQSLEIQSLEEIDSGLQSSSLKSNSFTTSDMNSDCGRGEDDDASEPS 854
            RESMDLDSHSESDTQSLEIQSLEEIDSGLQSSSLKSNSFTTSDMNSDCGRGEDDDASEPS
Sbjct: 781  RESMDLDSHSESDTQSLEIQSLEEIDSGLQSSSLKSNSFTTSDMNSDCGRGEDDDASEPS 840

Query: 855  KMLVSAEASSNLRTAQSDGSACHEDGTCSFKGFDIAKDPLDHYFLGTNGQTINGRKWLKK 914
            KMLVSAEASSNLRTAQSDGSACHEDGTCSFKGFDIAKDPLDHYFLGTNGQTINGRKWLKK
Sbjct: 841  KMLVSAEASSNLRTAQSDGSACHEDGTCSFKGFDIAKDPLDHYFLGTNGQTINGRKWLKK 900

Query: 915  IQQDWTILQNNLPDGIYVRVYEDRMDLLRTVIVGAYGTPYQDGLFFFDFHLPPEYPDVPP 974
            IQQDWTILQNNLPDGIYVRVYEDRMDLLRTVIVGAYGTPYQDGLFFFDFHLPPEYPDVPP
Sbjct: 901  IQQDWTILQNNLPDGIYVRVYEDRMDLLRTVIVGAYGTPYQDGLFFFDFHLPPEYPDVPP 960

Query: 975  SAYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSSILQVLVSLQGLVLNSKP 1034
            SAYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSSILQVLVSLQGLVLNSKP
Sbjct: 961  SAYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSSILQVLVSLQGLVLNSKP 1020

Query: 1035 YFNEAGYDKQIGTAEGEKNSLSYNENTFLLNCKTIMYLMRKPPKDFEELVKEHFRRRGYY 1094
            YFNEAGYDKQIGTAEGEKNSLSYNENTFLLNCKTIMYLMRKPPKDFEELVKEHFRRRGYY
Sbjct: 1021 YFNEAGYDKQIGTAEGEKNSLSYNENTFLLNCKTIMYLMRKPPKDFEELVKEHFRRRGYY 1080

Query: 1095 ILKACDAYMKGHLIGSLTEDASIREESDPNSTSVGFKLMLAKIVPKLFSSLNEVGADCRD 1154
            ILKACDAYMKGHLIGSLTEDASIREESDPNSTSVGFKLMLAKIVPKLFSSLNEVGADCRD
Sbjct: 1081 ILKACDAYMKGHLIGSLTEDASIREESDPNSTSVGFKLMLAKIVPKLFSSLNEVGADCRD 1140

Query: 1155 FKHFQQL 1162
            FKHFQQL
Sbjct: 1141 FKHFQQL 1147

BLAST of Carg05185-RA vs. NCBI nr
Match: XP_023534561.1 (probable ubiquitin-conjugating enzyme E2 23 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 2081.6 bits (5392), Expect = 0.0e+00
Identity = 1102/1147 (96.08%), Postives = 1117/1147 (97.38%), Query Frame = 0

Query: 15   MGQQDSVDEESLTDTCDSYMNRDGVSTSGNIHMPTINSDEARRMNENRSDKHNIPHVYRR 74
            M Q DSVD+ESLTDTCDSYMNRDGVSTSGNIHMPTIN+DEARRMNEN+S+K NIPHVYRR
Sbjct: 1    MAQPDSVDKESLTDTCDSYMNRDGVSTSGNIHMPTINNDEARRMNENKSNKPNIPHVYRR 60

Query: 75   DIVKSKGSGMIGIVTEVAGDADSDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 134
            DIVKSKGSGMIGIVTEVAGDA   XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 61   DIVKSKGSGMIGIVTEVAGDAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 120

Query: 135  XXXXXXXXXXXXXXXXXXXQPLPADEVRVLWMDESETTQNVNDLTVIDRGFLHGDFVAAV 194
            XXXXXXXXX          QPLPADEVRVLWMDESETTQNVNDLTVIDRGFLHGDFVAAV
Sbjct: 121  XXXXXXXXXKNSNRDSYKNQPLPADEVRVLWMDESETTQNVNDLTVIDRGFLHGDFVAAV 180

Query: 195  SNPTGQAGVVVDVNISVDLLVPDGSVVKNISSKDLIRVRDFAVGDYVVLGPWLGRIDDVL 254
            SNPTGQ+GVVVDVNISVDLLVPDGSVVKNISSKDLIRVRDFAVGDYVVLGPWLGRIDDVL
Sbjct: 181  SNPTGQSGVVVDVNISVDLLVPDGSVVKNISSKDLIRVRDFAVGDYVVLGPWLGRIDDVL 240

Query: 255  DNVTVMFDDGSKCKVTKAEPSRLKPVSKNILEDASFPYYPGQRVRASSSTVFKNSKWLSG 314
            DNVTVMFDDGSKCKVTKAEPSRLKPVSKNILEDASFPYYPGQR+RASSSTVFKNSKWLSG
Sbjct: 241  DNVTVMFDDGSKCKVTKAEPSRLKPVSKNILEDASFPYYPGQRLRASSSTVFKNSKWLSG 300

Query: 315  LWKPNRLQGTVTKVTVGSVFIYWIASAGYGPDSSTTPAEEQSPKNLRLLTCFSHVNWQLG 374
            LWKPNRLQGTVTKVTVGSVFIYWIASAGYGPDSSTTPAEEQSPKNLRLLTCFSHVNWQLG
Sbjct: 301  LWKPNRLQGTVTKVTVGSVFIYWIASAGYGPDSSTTPAEEQSPKNLRLLTCFSHVNWQLG 360

Query: 375  DWCLLPSSFSSGSTKDLSQTELSDSVTNTLDYAQTVGACDSEDTVVDELSGRTESTDLDS 434
            DWCLLPSSFSSGSTKD SQTEL DSVTNTLDYAQTVGACDSEDTVVDEL+GRTESTDLDS
Sbjct: 361  DWCLLPSSFSSGSTKDSSQTELPDSVTNTLDYAQTVGACDSEDTVVDELTGRTESTDLDS 420

Query: 435  ISACNGNYRDPECNILPESSSSRATKENTHETWPLHRKKIRKVVIRRDKXXXXXXXNFER 494
            ISACNGNYRDPECNILPESSSSRATKENTHETWPLHRKKIRKVVIRRDKXXXXXXXNFER
Sbjct: 421  ISACNGNYRDPECNILPESSSSRATKENTHETWPLHRKKIRKVVIRRDKXXXXXXXNFER 480

Query: 495  ALLIINTTTKVDVVWQDGRTELALDSTGLIPINPGDHEFVPEQYVVEKATDNDDDVTESR 554
            ALLIINTTTKVDVVWQDGR EL LDSTGLIPINPGDHEFVPEQYVVEKA+DNDDDVTESR
Sbjct: 481  ALLIINTTTKVDVVWQDGRAELVLDSTGLIPINPGDHEFVPEQYVVEKASDNDDDVTESR 540

Query: 555  RVGVVKSVHAKERTACVRWLKPVSRAEDPREFDIEEVVSVYELEGHPDYDYCYGDVVVRL 614
            RVGVVKSVHAKERTACVRWLKPVSRAEDPREFDIEEVVSVYELEGHPDYDYCYGDVVVRL
Sbjct: 541  RVGVVKSVHAKERTACVRWLKPVSRAEDPREFDIEEVVSVYELEGHPDYDYCYGDVVVRL 600

Query: 615  SPVSDSIEALSSENNTEELNQQSSTNGVMSCPEKVSGSLKIEYPSSSDDCFDFSDLSWVG 674
            SPVSDSIEALSSEN TEELN QSSTNGVMSC EKVSGSLKIEYPSSSDDCFDFSDLSWVG
Sbjct: 601  SPVSDSIEALSSENTTEELNHQSSTNGVMSCTEKVSGSLKIEYPSSSDDCFDFSDLSWVG 660

Query: 675  NITNLKNGDIEVFWANGMVSTVGPQAIYVVGRDDDDESTGAGSEISNGAASWETVXXXXX 734
            NITNLKNGDIEVFWANGMVSTVGPQAIYVVGRDDDDES GAGSE+SNGAASWETV  XXX
Sbjct: 661  NITNLKNGDIEVFWANGMVSTVGPQAIYVVGRDDDDESIGAGSEVSNGAASWETVDDXXX 720

Query: 735  XMDVENAKEDIELQDTGVDSEEEESEQHNSGRSLALSVPLAAFRFVTRLATGIFSRGPRN 794
             MDVENAKEDIELQDTGVDSEEEE+EQHNSGRSLALSVPLAAFRFVTRLATG+FSRGPRN
Sbjct: 721  EMDVENAKEDIELQDTGVDSEEEENEQHNSGRSLALSVPLAAFRFVTRLATGLFSRGPRN 780

Query: 795  RESMDLDSHSESDTQSLEIQSLEEIDSGLQSSSLKSNSFTTSDMNSDCGRGEDDDASEPS 854
            R+SMDLDSHSESDTQSLEIQ+LEEIDSGLQSSSLKSNSFT SD+N DCGRGEDDDASEPS
Sbjct: 781  RDSMDLDSHSESDTQSLEIQALEEIDSGLQSSSLKSNSFTASDLNPDCGRGEDDDASEPS 840

Query: 855  KMLVSAEASSNLRTAQSDGSACHEDGTCSFKGFDIAKDPLDHYFLGTNGQTINGRKWLKK 914
            K+LVSAEASSNLRTAQSDGSACHEDGTCSFKGFDI KDPLDHYFLGTNGQTINGRKWLKK
Sbjct: 841  KVLVSAEASSNLRTAQSDGSACHEDGTCSFKGFDIVKDPLDHYFLGTNGQTINGRKWLKK 900

Query: 915  IQQDWTILQNNLPDGIYVRVYEDRMDLLRTVIVGAYGTPYQDGLFFFDFHLPPEYPDVPP 974
            IQQDWTILQNNLPDGIYVRVYEDRMDLLRTVIVGAYGTPYQDGLFFFDFHLPPEYPDVPP
Sbjct: 901  IQQDWTILQNNLPDGIYVRVYEDRMDLLRTVIVGAYGTPYQDGLFFFDFHLPPEYPDVPP 960

Query: 975  SAYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSSILQVLVSLQGLVLNSKP 1034
            SAYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSSILQVLVSLQGLVLNSKP
Sbjct: 961  SAYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSSILQVLVSLQGLVLNSKP 1020

Query: 1035 YFNEAGYDKQIGTAEGEKNSLSYNENTFLLNCKTIMYLMRKPPKDFEELVKEHFRRRGYY 1094
            YFNEAGYDKQIGTAEGEKNSLSYNENTFLLNCKTIMYLMRKPPKDFEELVKEHFRRRGYY
Sbjct: 1021 YFNEAGYDKQIGTAEGEKNSLSYNENTFLLNCKTIMYLMRKPPKDFEELVKEHFRRRGYY 1080

Query: 1095 ILKACDAYMKGHLIGSLTEDASIREESDPNSTSVGFKLMLAKIVPKLFSSLNEVGADCRD 1154
            ILKACDAYMKGHLIGSLTEDASIREESDPNSTSVGFKLMLAKIVPKLFSSLNEVGADCRD
Sbjct: 1081 ILKACDAYMKGHLIGSLTEDASIREESDPNSTSVGFKLMLAKIVPKLFSSLNEVGADCRD 1140

Query: 1155 FKHFQQL 1162
            FKHFQQL
Sbjct: 1141 FKHFQQL 1147

BLAST of Carg05185-RA vs. NCBI nr
Match: XP_022970910.1 (probable ubiquitin-conjugating enzyme E2 23 [Cucurbita maxima])

HSP 1 Score: 2060.8 bits (5338), Expect = 0.0e+00
Identity = 1089/1149 (94.78%), Postives = 1105/1149 (96.17%), Query Frame = 0

Query: 15   MGQQDSVDEESLTDTCDSYMNRDGVSTSGNIHMPTINSDEARRMNENRSDKHNIPHVYRR 74
            MGQQDSVD+ESLTDTCDSYMNRDGVSTSGNIHMPTIN+DEARRMNEN+SDKHN+PHVYRR
Sbjct: 1    MGQQDSVDKESLTDTCDSYMNRDGVSTSGNIHMPTINNDEARRMNENKSDKHNVPHVYRR 60

Query: 75   DIVKSKGSGMIGIVTEVAGDADSDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 134
            DIVKSKGSGMIGIVTEVAGDAD   XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 61   DIVKSKGSGMIGIVTEVAGDAD---XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 120

Query: 135  XXXXXXXXXXXXXXXXXXXQPLPADEVRVLWMDESETTQNVNDLTVIDRGFLHGDFVAAV 194
            XXXXXX             QPL ADEVRVLWMDESETTQNVNDLTVIDRGFLHGDFVAAV
Sbjct: 121  XXXXXXTNAKNSNRDSYKNQPLTADEVRVLWMDESETTQNVNDLTVIDRGFLHGDFVAAV 180

Query: 195  SNPTGQAGVVVDVNISVDLLVPDGSVVKNISSKDLIRVRDFAVGDYVVLGPWLGRIDDVL 254
            SNPTGQAGVVVDVNISVDLLVPDGSVVKNISSKDLIRVRDF VGDYVVLGPWLGRIDD+L
Sbjct: 181  SNPTGQAGVVVDVNISVDLLVPDGSVVKNISSKDLIRVRDFTVGDYVVLGPWLGRIDDIL 240

Query: 255  DNVTVMFDDGSKCKVTKAEPSRLKPVSKNILEDASFPYYPGQRVRASSSTVFKNSKWLSG 314
            DNVTVMFDDGSKCKVTKAEPSRLKPVSKNILEDASFPYYPGQRVRASSSTVFKNSKWLSG
Sbjct: 241  DNVTVMFDDGSKCKVTKAEPSRLKPVSKNILEDASFPYYPGQRVRASSSTVFKNSKWLSG 300

Query: 315  LWKPNRLQGTVTKVTVGSVFIYWIASAGYGPDSSTTPAEEQSPKNLRLLTCFSHVNWQLG 374
            LWKPNRLQGTVTKVTVGSVFIYWIASAGYGPDSSTTPAEEQSPKNLRLLTCFSHVNWQLG
Sbjct: 301  LWKPNRLQGTVTKVTVGSVFIYWIASAGYGPDSSTTPAEEQSPKNLRLLTCFSHVNWQLG 360

Query: 375  DWCLLPSSFSSGSTKDLSQTELSDSVTNTLDYAQTVGACDSEDTVVDELSGRTESTDLDS 434
            DWCLLPSSFSSGSTKD SQTELSDSVTNTLDYAQTVGACDSEDTV+DEL+GRTESTDLDS
Sbjct: 361  DWCLLPSSFSSGSTKDSSQTELSDSVTNTLDYAQTVGACDSEDTVLDELNGRTESTDLDS 420

Query: 435  ISACNGNYRDPECNILPESSSSRATKENTHETWPLHRKKIRKVVIRRDKXXXXXXXNFER 494
            ISACNGNYRDPEC+ILPESSSSRATKENTHETWPLHRKKIRKVVIRRDK       NFER
Sbjct: 421  ISACNGNYRDPECHILPESSSSRATKENTHETWPLHRKKIRKVVIRRDKKARKKDDNFER 480

Query: 495  ALLIINTTTKVDVVWQDGRTELALDSTGLIPINPGDHEFVPEQYVVEKATDNDDDVTESR 554
            ALLIINT+TKVDVVWQDGRTELALDSTGLIPINPGDHEFVPEQYVVEKA+DNDDDVTESR
Sbjct: 481  ALLIINTSTKVDVVWQDGRTELALDSTGLIPINPGDHEFVPEQYVVEKASDNDDDVTESR 540

Query: 555  RVGVVKSVHAKERTACVRWLKPVSRAEDPREFDIEEVVSVYELEGHPDYDYCYGDVVVRL 614
            RVGVVKSVHA ERTACVRWLKPVSRAEDPREFDIEEVVSVYELEGHPDYDYCYGDVVVRL
Sbjct: 541  RVGVVKSVHANERTACVRWLKPVSRAEDPREFDIEEVVSVYELEGHPDYDYCYGDVVVRL 600

Query: 615  SPVSDSIEALSSENNTEELNQQSSTNGVMSCPEKVSGSLKIEYPSSSDDCFDFSDLSWVG 674
            SPVSDSIEALSSENNTEELN QSSTNGVMSC EKVSGSLKIEYPSSSDD FDFSDLSWVG
Sbjct: 601  SPVSDSIEALSSENNTEELNHQSSTNGVMSCTEKVSGSLKIEYPSSSDDFFDFSDLSWVG 660

Query: 675  NITNLKNGDIEVFWANGMVSTVGPQAIYVVGRDDDDESTGAGSEISNGAASWETV--XXX 734
            NITNLKNGDIEVFWANGMVSTVGPQAIYVVGRDDDDES GAGSEISNGAASWET+  XXX
Sbjct: 661  NITNLKNGDIEVFWANGMVSTVGPQAIYVVGRDDDDESIGAGSEISNGAASWETIXXXXX 720

Query: 735  XXXMDVENAKEDIELQDTGVDSEEEESEQHNSGRSLALSVPLAAFRFVTRLATGIFSRGP 794
            XXX  VENAKEDIELQDTGVDSE     QHNSGRSLALSVPLAAFRFVTRLATGIFSRGP
Sbjct: 721  XXXXXVENAKEDIELQDTGVDSEXXXXXQHNSGRSLALSVPLAAFRFVTRLATGIFSRGP 780

Query: 795  RNRESMDLDSHSESDTQSLEIQSLEEIDSGLQSSSLKSNSFTTSDMNSDCGRGEDDDASE 854
            RNRESMDLDSHSESDTQSLEIQ+LEEIDSGLQSSSLKSNSFT SDMNSDCGRGEDDDASE
Sbjct: 781  RNRESMDLDSHSESDTQSLEIQALEEIDSGLQSSSLKSNSFTASDMNSDCGRGEDDDASE 840

Query: 855  PSKMLVSAEASSNLRTAQSDGSACHEDGTCSFKGFDIAKDPLDHYFLGTNGQTINGRKWL 914
            PSK+LVSAEASSNLRTAQSDGS CHEDGTCSFKGFDIAKDPLDHYFLGTNGQTINGRKWL
Sbjct: 841  PSKVLVSAEASSNLRTAQSDGSTCHEDGTCSFKGFDIAKDPLDHYFLGTNGQTINGRKWL 900

Query: 915  KKIQQDWTILQNNLPDGIYVRVYEDRMDLLRTVIVGAYGTPYQDGLFFFDFHLPPEYPDV 974
            KKIQQDWTILQNNLPDGIYV+VYEDRMDLLR VIVGAYGTPYQDGLFFFDFHLPP+YPDV
Sbjct: 901  KKIQQDWTILQNNLPDGIYVQVYEDRMDLLRAVIVGAYGTPYQDGLFFFDFHLPPDYPDV 960

Query: 975  PPSAYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSSILQVLVSLQGLVLNS 1034
            PPSAYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSSILQVLVSLQGLVLNS
Sbjct: 961  PPSAYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSSILQVLVSLQGLVLNS 1020

Query: 1035 KPYFNEAGYDKQIGTAEGEKNSLSYNENTFLLNCKTIMYLMRKPPKDFEELVKEHFRRRG 1094
            KPYFNEAGYDKQIGTAEGEKNSLSYNENTFLLNCKTIMYLMRKPPKDFEELVKEHFR+RG
Sbjct: 1021 KPYFNEAGYDKQIGTAEGEKNSLSYNENTFLLNCKTIMYLMRKPPKDFEELVKEHFRKRG 1080

Query: 1095 YYILKACDAYMKGHLIGSLTEDASIREESDPNSTSVGFKLMLAKIVPKLFSSLNEVGADC 1154
            YYILKACDAYMKGHLIGSLTEDASIREESDPNSTSVGFKLMLAKIVPKLFSSLNEV ADC
Sbjct: 1081 YYILKACDAYMKGHLIGSLTEDASIREESDPNSTSVGFKLMLAKIVPKLFSSLNEVRADC 1140

Query: 1155 RDFKHFQQL 1162
            RDFKHFQQL
Sbjct: 1141 RDFKHFQQL 1146

BLAST of Carg05185-RA vs. NCBI nr
Match: XP_023534560.1 (probable ubiquitin-conjugating enzyme E2 23 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 2038.5 bits (5280), Expect = 0.0e+00
Identity = 1092/1164 (93.81%), Postives = 1106/1164 (95.02%), Query Frame = 0

Query: 15   MGQQDSVDEESLTDTCDSYMNRDGVSTSGNIHMPTINSDEARRMNENRSDKHNIPHVYRR 74
            M Q DSVD+ESLTDTCDSYMNRDGVSTSGNIHMPTIN+DEARRMNEN+S+K NIPHVYRR
Sbjct: 1    MAQPDSVDKESLTDTCDSYMNRDGVSTSGNIHMPTINNDEARRMNENKSNKPNIPHVYRR 60

Query: 75   DIVKSKGSGMIGIVTEVAGDADSDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 134
            DIVKSKGSGMIGIVTEVAGDA   XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 61   DIVKSKGSGMIGIVTEVAGDAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 120

Query: 135  XXXXXXXXXXXXXXXXXXXQPLPADEVRVLWMDESETTQNVNDLTVIDRGFLHGDFVAAV 194
            XXXXXXXXX          QPLPADEVRVLWMDESETTQNVNDLTVIDRGFLHGDFVAAV
Sbjct: 121  XXXXXXXXXKNSNRDSYKNQPLPADEVRVLWMDESETTQNVNDLTVIDRGFLHGDFVAAV 180

Query: 195  SNPTGQAGVVVDVNISVDLLVPDGSVVKNISSKDLIRVRDFAVGDYVVLGPWLGRIDDVL 254
            SNPTGQ+GVVVDVNISVDLLVPDGSVVKNISSKDLIRVRDFAVGDYVVLGPWLGRIDDVL
Sbjct: 181  SNPTGQSGVVVDVNISVDLLVPDGSVVKNISSKDLIRVRDFAVGDYVVLGPWLGRIDDVL 240

Query: 255  DNVTVMFDDGSKCKVTKAEPSRLKPVSKNILEDASFPYYPGQRVRASSSTVFKNSKWLSG 314
            DNVTVMFDDGSKCKVTKAEPSRLKPVSKNILEDASFPYYPGQR+RASSSTVFKNSKWLSG
Sbjct: 241  DNVTVMFDDGSKCKVTKAEPSRLKPVSKNILEDASFPYYPGQRLRASSSTVFKNSKWLSG 300

Query: 315  LWKPNRLQGTVTKVTVGSVFIYWIASAGYGPDSSTTPAEEQSPKNLRLLTCFSHVNWQLG 374
            LWKPNRLQGTVTKVTVGSVFIYWIASAGYGPDSSTTPAEEQSPKNLRLLTCFSHVNWQLG
Sbjct: 301  LWKPNRLQGTVTKVTVGSVFIYWIASAGYGPDSSTTPAEEQSPKNLRLLTCFSHVNWQLG 360

Query: 375  DWCLLPSSFSSGSTKDLSQTELSDSVTNTLDYAQTVGACDSEDTVVDELSGRTESTDLDS 434
            DWCLLPSSFSSGSTKD SQTEL DSVTNTLDYAQTVGACDSEDTVVDEL+GRTESTDLDS
Sbjct: 361  DWCLLPSSFSSGSTKDSSQTELPDSVTNTLDYAQTVGACDSEDTVVDELTGRTESTDLDS 420

Query: 435  ISACNGNYRDPECNILPESSSSRATKENTHETWPLHRKKIRKVVIRRDKXXXXXXXNFER 494
            ISACNGNYRDPECNILPESSSSRATKENTHETWPLHRKKIRKVVIRRDKXXXXXXXNFER
Sbjct: 421  ISACNGNYRDPECNILPESSSSRATKENTHETWPLHRKKIRKVVIRRDKXXXXXXXNFER 480

Query: 495  ALLIINTTTKVDVVWQDGRTELALDSTGLIPINPGDHEFVPEQYVVEKATDNDDDVTESR 554
            ALLIINTTTKVDVVWQDGR EL LDSTGLIPINPGDHEFVPEQYVVEKA+DNDDDVTESR
Sbjct: 481  ALLIINTTTKVDVVWQDGRAELVLDSTGLIPINPGDHEFVPEQYVVEKASDNDDDVTESR 540

Query: 555  RVGVVKSVHAKERTACVRWLKPVSRAEDPREFDIEEVVSVYELEGHPDYDYCYGDVVVRL 614
            RVGVVKSVHAKERTACVRWLKPVSRAEDPREFDIEEVVSVYELEGHPDYDYCYGDVVVRL
Sbjct: 541  RVGVVKSVHAKERTACVRWLKPVSRAEDPREFDIEEVVSVYELEGHPDYDYCYGDVVVRL 600

Query: 615  SPVSDSIEALSSENNTEELNQQSSTNGVMSCPEKVSGSLKIEYPSSSDDCFDFSDLSWVG 674
            SPVSDSIEALSSEN TEELN QSSTNGVMSC EKVSGSLKIEYPSSSDDCFDFSDLSWVG
Sbjct: 601  SPVSDSIEALSSENTTEELNHQSSTNGVMSCTEKVSGSLKIEYPSSSDDCFDFSDLSWVG 660

Query: 675  NITNLKNGDIEVFWANGMVSTVGPQAIYVVGRDDDDESTGAGSEISNGA----------- 734
            NITNLKNGDIEVFWANGMVSTVGPQAIYVVGRDDDDES GAGSE+SNGA           
Sbjct: 661  NITNLKNGDIEVFWANGMVSTVGPQAIYVVGRDDDDESIGAGSEVSNGAXXXXXXXXXXX 720

Query: 735  ------ASWETVXXXXXXMDVENAKEDIELQDTGVDSEEEESEQHNSGRSLALSVPLAAF 794
                        XXXXXX  VENAKEDIELQDTGVDS      QHNSGRSLALSVPLAAF
Sbjct: 721  XXXXXXXXXXXXXXXXXXXXVENAKEDIELQDTGVDSXXXXXXQHNSGRSLALSVPLAAF 780

Query: 795  RFVTRLATGIFSRGPRNRESMDLDSHSESDTQSLEIQSLEEIDSGLQSSSLKSNSFTTSD 854
            RFVTRLATG+FSRGPRNR+SMDLDSHSESDTQSLEIQ+LEEIDSGLQSSSLKSNSFT SD
Sbjct: 781  RFVTRLATGLFSRGPRNRDSMDLDSHSESDTQSLEIQALEEIDSGLQSSSLKSNSFTASD 840

Query: 855  MNSDCGRGEDDDASEPSKMLVSAEASSNLRTAQSDGSACHEDGTCSFKGFDIAKDPLDHY 914
            +N DCGRGEDDDASEPSK+LVSAEASSNLRTAQSDGSACHEDGTCSFKGFDI KDPLDHY
Sbjct: 841  LNPDCGRGEDDDASEPSKVLVSAEASSNLRTAQSDGSACHEDGTCSFKGFDIVKDPLDHY 900

Query: 915  FLGTNGQTINGRKWLKKIQQDWTILQNNLPDGIYVRVYEDRMDLLRTVIVGAYGTPYQDG 974
            FLGTNGQTINGRKWLKKIQQDWTILQNNLPDGIYVRVYEDRMDLLRTVIVGAYGTPYQDG
Sbjct: 901  FLGTNGQTINGRKWLKKIQQDWTILQNNLPDGIYVRVYEDRMDLLRTVIVGAYGTPYQDG 960

Query: 975  LFFFDFHLPPEYPDVPPSAYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSS 1034
            LFFFDFHLPPEYPDVPPSAYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSS
Sbjct: 961  LFFFDFHLPPEYPDVPPSAYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSS 1020

Query: 1035 ILQVLVSLQGLVLNSKPYFNEAGYDKQIGTAEGEKNSLSYNENTFLLNCKTIMYLMRKPP 1094
            ILQVLVSLQGLVLNSKPYFNEAGYDKQIGTAEGEKNSLSYNENTFLLNCKTIMYLMRKPP
Sbjct: 1021 ILQVLVSLQGLVLNSKPYFNEAGYDKQIGTAEGEKNSLSYNENTFLLNCKTIMYLMRKPP 1080

Query: 1095 KDFEELVKEHFRRRGYYILKACDAYMKGHLIGSLTEDASIREESDPNSTSVGFKLMLAKI 1154
            KDFEELVKEHFRRRGYYILKACDAYMKGHLIGSLTEDASIREESDPNSTSVGFKLMLAKI
Sbjct: 1081 KDFEELVKEHFRRRGYYILKACDAYMKGHLIGSLTEDASIREESDPNSTSVGFKLMLAKI 1140

Query: 1155 VPKLFSSLNEVGADCRDFKHFQQL 1162
            VPKLFSSLNEVGADCRDFKHFQQL
Sbjct: 1141 VPKLFSSLNEVGADCRDFKHFQQL 1164

BLAST of Carg05185-RA vs. NCBI nr
Match: XP_022986085.1 (probable ubiquitin-conjugating enzyme E2 23 isoform X2 [Cucurbita maxima])

HSP 1 Score: 1875.9 bits (4858), Expect = 0.0e+00
Identity = 995/1148 (86.67%), Postives = 1046/1148 (91.11%), Query Frame = 0

Query: 15   MGQQDSVDEESLTDTCDSYMNRDGVSTSGNIHMPTINSDEARRMNENRSDKHNIPHVYRR 74
            M QQ SVD+ESL  T +SY NRDGVS+S N+HM TIN+DE RR NEN SDK ++PH+YR+
Sbjct: 1    MEQQGSVDKESLIGTGNSYTNRDGVSSSENVHMTTINNDETRRTNENTSDKPSVPHIYRQ 60

Query: 75   DIVKSKGSGMIGIVTEVAGDADSDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 134
            DIVKSKGSGMIGIVTEVAGD    XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 61   DIVKSKGSGMIGIVTEVAGDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 120

Query: 135  XXXXXXXXXXXXXXXXXXXQPLPADEVRVLWMDESETTQNVNDLTVIDRGFLHGDFVAAV 194
            XXXXXXXX           QPLP DEVRVLWMDESETT NVNDLTVIDRGFLHGDFVAAV
Sbjct: 121  XXXXXXXXAKNSNRDNYKSQPLPDDEVRVLWMDESETTLNVNDLTVIDRGFLHGDFVAAV 180

Query: 195  SNPTGQAGVVVDVNISVDLLVPDGSVVKNISSKDLIRVRDFAVGDYVVLGPWLGRIDDVL 254
            S+PTGQAGVVVDVNISVDLLVPDGS++K+ISSKDL RVRDF VGDYVVLGPWLGRIDDVL
Sbjct: 181  SDPTGQAGVVVDVNISVDLLVPDGSIMKDISSKDLKRVRDFTVGDYVVLGPWLGRIDDVL 240

Query: 255  DNVTVMFDDGSKCKVTKAEPSRLKPVSKNILEDASFPYYPGQRVRASSSTVFKNSKWLSG 314
            DNVTVMFDDGSKCKVTKAEP RLKPVSKNILEDA+FPYYPGQRV+ASSSTVFKNSKWLSG
Sbjct: 241  DNVTVMFDDGSKCKVTKAEPLRLKPVSKNILEDANFPYYPGQRVKASSSTVFKNSKWLSG 300

Query: 315  LWKPNRLQGTVTKVTVGSVFIYWIASAGYGPDSSTTPAEEQSPKNLRLLTCFSHVNWQLG 374
            LWKPNRL+GTVTKVTVGSVFIYWIASAGYGPDSSTTPAEEQ+PKNLRLLTCFSH NWQLG
Sbjct: 301  LWKPNRLEGTVTKVTVGSVFIYWIASAGYGPDSSTTPAEEQTPKNLRLLTCFSHANWQLG 360

Query: 375  DWCLLPSSFSSGSTKDLSQTELSDSVTNTLDYAQTVGACDSEDTVVDELSGRTESTDLDS 434
            DWCLLPSSFSSG TK+ SQT  SDSVTN+LD  Q    CDSED V++ELSG TESTDLDS
Sbjct: 361  DWCLLPSSFSSGLTKESSQTHRSDSVTNSLDSTQPEVVCDSEDVVLNELSGTTESTDLDS 420

Query: 435  ISACNGNYRDPECNILPESSSSRATKENTHETWPLHRKKIRKVVIRRDKXXXXXXXNFER 494
            +SAC+GNYR+PECNILPESS+SRA KE +HETWPLHRKKIRKVVIRRDK       NFER
Sbjct: 421  LSACDGNYRNPECNILPESSNSRALKETSHETWPLHRKKIRKVVIRRDKKARKKEENFER 480

Query: 495  ALLIINTTTKVDVVWQDGRTELALDSTGLIPI-NPGDHEFVPEQYVVEKATDNDDDVTES 554
            ALLIINT TKVDV WQDGRTE  LDST LIPI NPGDHEFVPEQYVVEKA+DNDDD++ES
Sbjct: 481  ALLIINTKTKVDVAWQDGRTECGLDSTTLIPIDNPGDHEFVPEQYVVEKASDNDDDISES 540

Query: 555  RRVGVVKSVHAKERTACVRWLKPVSRAEDPREFDIEEVVSVYELEGHPDYDYCYGDVVVR 614
            RRVGVVKSVHAKERTACV WLKPVSRAEDPREFDI+E+VSVYELEGHPDYDYCYGDVVVR
Sbjct: 541  RRVGVVKSVHAKERTACVMWLKPVSRAEDPREFDIQEIVSVYELEGHPDYDYCYGDVVVR 600

Query: 615  LSPVSDSIEALSSENNTEELNQQSSTNGVMSCPEKVSGSLKIEYPSSSDDCFDFSDLSWV 674
            LSPVSDSIE++S  NNTEEL QQSSTN +M+C EK SGS KIE PS SDDC DFSDLSWV
Sbjct: 601  LSPVSDSIESISLGNNTEELKQQSSTNEMMNCTEKASGSQKIEDPSCSDDCIDFSDLSWV 660

Query: 675  GNITNLKNGDIEVFWANGMVSTVGPQAIYVVGRDDDDESTGAGSEISNGAASWETVXXXX 734
            GNIT LKNGDIEV WANGMVSTVGPQAIYVVGRDDDDES  AGSE+SNGAASWETV    
Sbjct: 661  GNITGLKNGDIEVTWANGMVSTVGPQAIYVVGRDDDDESIAAGSEVSNGAASWETVDDDE 720

Query: 735  XXMDVENAKEDIELQDTGVDSEEEESEQHNSGRSLALSVPLAAFRFVTRLATGIFSRGPR 794
                V+NAKEDIELQD GV+SEEE SEQ+NSGR+LALSVP AA RF TRLA GIFSRGPR
Sbjct: 721  RD-SVDNAKEDIELQDPGVNSEEEGSEQNNSGRNLALSVPFAAIRFFTRLAAGIFSRGPR 780

Query: 795  NRESMDLDSHSESDTQSLEIQSLEEIDSGLQSSSLKSNSFTTSDMNSDCGRGEDDDASEP 854
            N E MD+DSHSES+TQSLEIQ+LEE DSGLQSSSLKSNSF  SDMNSDCGRGED+ ASEP
Sbjct: 781  NPELMDVDSHSESETQSLEIQALEEKDSGLQSSSLKSNSFDASDMNSDCGRGEDNIASEP 840

Query: 855  SKMLVSAEASSNLRTAQSDGSACHEDGTCSFKGFDIAKDPLDHYFLGTNGQTINGRKWLK 914
            S++L SA+ASSNLRTA+SDGSACHEDGTCSFKGFDIAKDPLDHYFLGTNGQT +GRKWLK
Sbjct: 841  SEVLESADASSNLRTAESDGSACHEDGTCSFKGFDIAKDPLDHYFLGTNGQTNSGRKWLK 900

Query: 915  KIQQDWTILQNNLPDGIYVRVYEDRMDLLRTVIVGAYGTPYQDGLFFFDFHLPPEYPDVP 974
            KIQQDW+ILQNNLPDGIYVRVYEDRMDLLR VIVGAYGTPYQDGLFFFDFHLPPEYPDVP
Sbjct: 901  KIQQDWSILQNNLPDGIYVRVYEDRMDLLRAVIVGAYGTPYQDGLFFFDFHLPPEYPDVP 960

Query: 975  PSAYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSSILQVLVSLQGLVLNSK 1034
            PSAYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWD KSSSILQVLVSLQGLVLNSK
Sbjct: 961  PSAYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWDSKSSSILQVLVSLQGLVLNSK 1020

Query: 1035 PYFNEAGYDKQIGTAEGEKNSLSYNENTFLLNCKTIMYLMRKPPKDFEELVKEHFRRRGY 1094
            PYFNEAGYDKQIGTAEGEKNSLSYNENTFLLNCKTIMYLMRKPPKDFEELVKEHFRRRGY
Sbjct: 1021 PYFNEAGYDKQIGTAEGEKNSLSYNENTFLLNCKTIMYLMRKPPKDFEELVKEHFRRRGY 1080

Query: 1095 YILKACDAYMKGHLIGSLTEDASIREESDPNSTSVGFKLMLAKIVPKLFSSLNEVGADCR 1154
            +ILKACDAYMKGHLIGSLTEDASIRE SDPNSTSVGFKLMLAKIVPKL SSLNEVGADC 
Sbjct: 1081 FILKACDAYMKGHLIGSLTEDASIRENSDPNSTSVGFKLMLAKIVPKLMSSLNEVGADCD 1140

Query: 1155 DFKHFQQL 1162
            DFKHFQQL
Sbjct: 1141 DFKHFQQL 1147

BLAST of Carg05185-RA vs. TAIR10
Match: AT2G16920.1 (ubiquitin-conjugating enzyme 23)

HSP 1 Score: 1225.7 bits (3170), Expect = 0.0e+00
Identity = 684/1137 (60.16%), Postives = 824/1137 (72.47%), Query Frame = 0

Query: 36   RDGVSTSGNIHMPTINSDEARRMNENRSDKHNIPHVYRRDIVKSKGSGMIGIVTEVAGDA 95
            +D   TS N+ + +   D    ++   S+    P++YR+DIVK+K +G +G+V+EVAGD 
Sbjct: 5    QDDPGTSTNVGVDSSVDDSMASLSICDSEH---PNIYRQDIVKNKKTGSVGVVSEVAGDX 64

Query: 96   DSDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQP 155
               XXXXXXXXXXXXXXXXXXXXXXXXX                                
Sbjct: 65   XXXXXXXXXXXXXXXXXXXXXXXXXXXXGKKASEENVVNGDGEKKADGNYKCG------A 124

Query: 156  LPADEVRVLWMDESETTQNVNDLTVIDRGFLHGDFVAAVSNPTGQAGVVVDVNISVDLLV 215
            L  D++RVLWMD +E  Q++ND+TVIDRGFLHGD+VA+ S PTGQ GVVVDVNISVDLL 
Sbjct: 125  LEGDQIRVLWMDNTEPVQDINDVTVIDRGFLHGDYVASASEPTGQVGVVVDVNISVDLLA 184

Query: 216  PDGSVVKNISSKDLIRVRDFAVGDYVVLGPWLGRIDDVLDNVTVMFDDGSKCKVTKAEPS 275
            PDGS+ K+IS+K+L RVRDFAVGDYVV GPWLGRIDDVLDNVTV+FDDGS CKV + EP 
Sbjct: 185  PDGSIHKDISTKNLKRVRDFAVGDYVVHGPWLGRIDDVLDNVTVLFDDGSMCKVLRVEPL 244

Query: 276  RLKPVSKNIL-EDASFPYYPGQRVRASSSTVFKNSKWLSGLWKPNRLQGTVTKVTVGSVF 335
            RLKP+ KN L EDA+FPYYPGQRV+ASSS+VFKNS+WLSGLWKPNRL+GTVTKVT GS+F
Sbjct: 245  RLKPIPKNNLEEDANFPYYPGQRVKASSSSVFKNSRWLSGLWKPNRLEGTVTKVTAGSIF 304

Query: 336  IYWIASAGYGPDSSTTPAEEQSPKNLRLLTCFSHVNWQLGDWCLLPSSFSSGST---KDL 395
            +YWIASAG+GPDSS +P EEQ+P NL LL+CF+H NWQ+GDWCLLPS   S +    K +
Sbjct: 305  VYWIASAGFGPDSSVSPPEEQNPSNLTLLSCFTHANWQVGDWCLLPSLNQSATIPLHKHV 364

Query: 396  SQTELSDSVTNTLDYAQTVGACDSEDTVVDELSGRTESTDLDSISACNGNYRDPECNILP 455
            S+  L DS     D  Q +G  D ED V DE+SG+ E   + + +       DP     P
Sbjct: 365  SKLRLYDS---QADRQQKIGR-DLED-VQDEVSGKVEPAGITAEALPKVTSDDP-----P 424

Query: 456  ESSSSRATKENTHETWPLHRKKIRKVVIRRDKXXXXXXXNFERALLIINTTTKVDVVWQD 515
            + + S  +KE  HE WPLHRKKIRK+VIR+DK       +FE+ALL++N+ T+VDV WQD
Sbjct: 425  QRNPS-VSKEPVHEPWPLHRKKIRKLVIRKDKKVKKKEESFEQALLVVNSRTRVDVSWQD 484

Query: 516  GRTELALDSTGLIPI-NPGDHEFVPEQYVVEKATDNDDDVTESRRVGVVKSVHAKERTAC 575
            G  E   ++  LIPI  PGDHEFV EQYVVEK +D+ D+ TE RR GVVK+V+AK+RTA 
Sbjct: 485  GTIECRREAITLIPIETPGDHEFVSEQYVVEKTSDDGDNTTEPRRAGVVKNVNAKDRTAS 544

Query: 576  VRWLKPVSRAEDPREFDIEEVVSVYELEGHPDYDYCYGDVVVRLSPVSDSIEALSSENNT 635
            VRWL P+ RAE+PREF+ EE+VSVYELEGHPDYDYCYGDVVVRLSP++ ++ A S  N+ 
Sbjct: 545  VRWLNPLRRAEEPREFEKEEIVSVYELEGHPDYDYCYGDVVVRLSPIAVALPASSPGNSF 604

Query: 636  EELNQQSSTNGVMSCPEKVSGSLKIEYPSSSDDCFDFSDLSWVGNITNLKNGDIEVFWAN 695
            EE  QQ   NG           + ++    ++   D S LSWVGNIT LK+GDIEV WA+
Sbjct: 605  EEATQQD--NGYQDSESHQEAKILVD-KEENEPSTDLSKLSWVGNITGLKDGDIEVTWAD 664

Query: 696  GMVSTVGPQAIYVVGRDDDDESTGAGSEISNGAASWETVXXXXXXMDVENAKEDIELQDT 755
            G +STVGP A+YVVGRDDDDES    SE S+ AASWET+       D +    +I  +D 
Sbjct: 665  GTISTVGPHAVYVVGRDDDDESVAGESETSD-AASWETLN------DDDRGAPEIPEEDL 724

Query: 756  GVDSEEEESE------QHNSGRSLALSVPLAAFRFVTRLATGIFSRGPRNRESMDLDSHS 815
            G  S  E +       +++SGR+ AL++PLAA  FVTRLA+GIFSR  ++     +DS S
Sbjct: 725  GRSSSIEGNSDADIYAENDSGRNGALALPLAAIEFVTRLASGIFSRARKS-----VDSSS 784

Query: 816  ESDTQSLEIQSLEEIDSGLQSSSLKSNSFTTSDMNSDCGRGEDDDASEPSKMLVSAEASS 875
               T     +  E  +   ++ SL   S +  ++  +C   E       +K ++S E S+
Sbjct: 785  SDYTVENVYKQAESTNPSDETDSLDDPSPSKVNVTDNC---ESKGTQANAKNILSGETST 844

Query: 876  NLRTAQSDGSACHEDGTCSFKGFDIAKDPLDHYFLGTNGQTINGRKWLKKIQQDWTILQN 935
             L     D     E  +CSF+ FDI++DPLDH+FLG +GQ    R+W KK+ QDW ILQN
Sbjct: 845  FLE--DEDKPVPSEGDSCSFRRFDISQDPLDHHFLGVDGQKTKERQWFKKVDQDWKILQN 904

Query: 936  NLPDGIYVRVYEDRMDLLRTVIVGAYGTPYQDGLFFFDFHLPPEYPDVPPSAYYHSGGWR 995
            NLPDGI+VR YEDRMDLLR VIVGA+GTPYQDGLFFFDFHLP +YP VPPSAYYHSGGWR
Sbjct: 905  NLPDGIFVRAYEDRMDLLRAVIVGAFGTPYQDGLFFFDFHLPSDYPSVPPSAYYHSGGWR 964

Query: 996  INPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSSILQVLVSLQGLVLNSKPYFNEAGYDKQ 1055
            +NPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSSILQVLVSLQGLVLNSKPYFNEAGYDKQ
Sbjct: 965  LNPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSSILQVLVSLQGLVLNSKPYFNEAGYDKQ 1024

Query: 1056 IGTAEGEKNSLSYNENTFLLNCKTIMYLMRKPPKDFEELVKEHFRRRGYYILKACDAYMK 1115
            +GTAEGEKNSL YNENTFLLNCKT+MYLMRKPPKDFEEL+K+HFR+RGYYILKACDAYMK
Sbjct: 1025 VGTAEGEKNSLGYNENTFLLNCKTMMYLMRKPPKDFEELIKDHFRKRGYYILKACDAYMK 1084

Query: 1116 GHLIGSLTEDAS-IREESDPNSTSVGFKLMLAKIVPKLFSSLNEVGADCRDFKHFQQ 1161
            G+LIGSLT+DAS I E S  NSTSVGFKLMLAKI PKLFS+L+EVGADC +F+H QQ
Sbjct: 1085 GYLIGSLTKDASVIDERSSANSTSVGFKLMLAKIAPKLFSALSEVGADCNEFQHLQQ 1101

BLAST of Carg05185-RA vs. TAIR10
Match: AT2G33770.1 (phosphate 2)

HSP 1 Score: 592.8 bits (1527), Expect = 4.5e-169
Identity = 364/1010 (36.04%), Postives = 537/1010 (53.17%), Query Frame = 0

Query: 170  ETTQNVNDLTVIDRGFLHGDFVAAVSNPTGQAGVVVDVNISVDLLVPDGSVVKNISSKDL 229
            ET   +++    +RGF++GD V + + P+GQ+G V+++++ V+L    G ++K + +K L
Sbjct: 41   ETIGKIDEFLSFERGFMYGDIVRSATEPSGQSGRVINIDMFVNLESTHGKIMKEVDTKRL 100

Query: 230  IRVRDFAVGDYVVLGPWLGRIDDVLDNVTVMFDDGSKCKVTKAEPSRLKPVSKNILEDAS 289
             ++R  ++ DYV+ GPW+GR+D +++ V+V  DDG+  +V      +L  +  N+LED+ 
Sbjct: 101  QKLRSISLSDYVINGPWVGRVDKIVERVSVTLDDGTNYEVLVDGQDKLVAIPPNLLEDSQ 160

Query: 290  FPYYPGQRVRASSSTVFKNSKWLSGLWKPNRLQGTVTKVTVGSVFIYWIAS-AGYGPDSS 349
            + YYPGQRV+   +   +++ WL G W+  ++ GTV  V  G V++ W+AS    G  + 
Sbjct: 161  YSYYPGQRVQVKLAHAPRSTTWLCGTWRGTQVMGTVCTVEAGLVYVDWVASIVMEGDRNL 220

Query: 350  TTPAEEQSPKNLRLLTCFSHVNWQLGDWCLLPSSFSSGSTKDLSQTELSDSVTNTLDYAQ 409
            T P   Q+P++L LL C SH +WQLGDWC+LP S         S  ++++  T       
Sbjct: 221  TAPQALQNPESLTLLPCVSHASWQLGDWCILPGS---------SHCDIAERQT------- 280

Query: 410  TVGACDSEDTVVDELSGRTESTDLDSISACNGNYRDPECNILPESSSSRATKENTHETWP 469
                                     +++A N N    EC+   +   +R  + +      
Sbjct: 281  ------------------------PNVAAYNLN----ECHKTFQKGFNRNMQNS------ 340

Query: 470  LHRKKIRKVVIRRDKXXXXXXXNFERALLIINTTTKVDVVWQDGRTELALDSTGLIPINP 529
                                    +   +I  T  KV V+WQDG   L +DS  L+P+  
Sbjct: 341  ----------------------GLDELFVITKTKMKVAVMWQDGSCSLGVDSQQLLPVGA 400

Query: 530  -GDHEFVPEQYVVEKATDNDDDVTESRRVGVVKSVHAKERTACVRWLKPVSR-AEDPREF 589
               H+F PEQ+VVEK T N      S++ GVVK+V+AKE+T  V+W   V + A    + 
Sbjct: 401  VNAHDFWPEQFVVEKETCN------SKKWGVVKAVNAKEQTVKVQWTIQVEKEATGCVDE 460

Query: 590  DIEEVVSVYELEGHPDYDYCYGDVVVRL------SPVSDSIEALSSENNTEELNQQSSTN 649
             +EE+VS YEL  HPD+ +C+ DVVV+L       P +D+I A  +++    L  +S  +
Sbjct: 461  VMEEIVSAYELLEHPDFGFCFSDVVVKLLPEGKFDPNADTIVATEAKH----LLTESDYS 520

Query: 650  GVMSCPEKVSGSLKIEYPSSSDDCFDFSDLSWVGNITNLKNGDIEVFWANGMVSTVGPQA 709
            G                            LS +G +T  KNG ++V WANG  S V P  
Sbjct: 521  GAYF-------------------------LSSIGVVTGFKNGSVKVKWANGSTSKVAPCE 580

Query: 710  IYVVGRDDDDESTGAGSEISNGAASWETVXXXXXXMDVENAKEDIELQDTGVDSEEE--- 769
            I+ + R +   S+   SE S                 V++  + I   D    + +E   
Sbjct: 581  IWKMERSEYSNSSTVSSEGS-----------------VQDLSQKISQSDEASSNHQETGL 640

Query: 770  -------ESEQHNSGRSLALSVPLAAFRFVTRLATGIF----SRGPRNRESMDLDSHSES 829
                   ES   N     +  +P AA  F+T LA+ +F    S    +  S   DS  +S
Sbjct: 641  VKLYSVGESCNENIPECSSFFLPKAAIGFITNLASSLFGYQGSTSVISSHSRCNDSEDQS 700

Query: 830  DTQSLEIQSLEEIDSGLQSSSLKSNSFTTSDMNSDCGRGEDDDASEPSKMLVSAEA-SSN 889
            D++ L  ++ E  D                  NS+   GE D  +    + +  +  +  
Sbjct: 701  DSEVLVQETAESYD------------------NSETNSGEVDMTTTMVNIPIEGKGINKT 760

Query: 890  LRTAQSDGSACHEDGTCSFKGFDIAKDPLDHYFLGTN---GQTINGRKWLKKIQQDWTIL 949
            L +   + S         F+ FD+  D  DH+FL ++    Q+   + W+KK+QQ+W+ L
Sbjct: 761  LDSTLLENSR----NQVRFRQFDMVNDCSDHHFLSSDKGLAQSQVTKSWVKKVQQEWSNL 820

Query: 950  QNNLPDGIYVRVYEDRMDLLRTVIVGAYGTPYQDGLFFFDFHLPPEYPDVPPSAYYHSGG 1009
            + NLP+ IYVRV E+RMDLLR  +VGA GTPY DGLFFFD  LPP+YP  PP  +YHSGG
Sbjct: 821  EANLPNTIYVRVCEERMDLLRAALVGAPGTPYHDGLFFFDIMLPPQYPHEPPMVHYHSGG 880

Query: 1010 WRINPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSSILQVLVSLQGLVLNSKPYFNEAGYD 1069
             R+NPNLYE G+VCLSLLNTW+G G EVW+  SSSILQ+L+S Q LVLN KPYFNEAGYD
Sbjct: 881  MRLNPNLYESGRVCLSLLNTWSGSGTEVWNAGSSSILQLLLSFQALVLNEKPYFNEAGYD 899

Query: 1070 KQIGTAEGEKNSLSYNENTFLLNCKTIMYLMRKPPKDFEELVKEHFRRRGYYILKACDAY 1129
            KQ+G AEGEKNS+SYNEN FL+ CK+++ ++RKPPK FE LVK+HF  R  ++L AC AY
Sbjct: 941  KQLGRAEGEKNSVSYNENAFLITCKSMISMLRKPPKHFEMLVKDHFTHRAQHVLAACKAY 899

Query: 1130 MKGHLIGSLTEDASIREESDPNSTSVGFKLMLAKIVPKLFSSLNEVGADC 1153
            M+G  +GS    A+++  S  NST  GFK+ML+K+ PKL  + +E+G DC
Sbjct: 1001 MEGVPVGS---SANLQGNSTTNST--GFKIMLSKLYPKLLEAFSEIGVDC 899

BLAST of Carg05185-RA vs. TAIR10
Match: AT3G15355.1 (ubiquitin-conjugating enzyme 25)

HSP 1 Score: 285.0 bits (728), Expect = 2.0e-76
Identity = 135/257 (52.53%), Postives = 175/257 (68.09%), Query Frame = 0

Query: 884  FKGFDIAKDPLDHYFL--GTNGQTINGRKWLKKIQQDWTILQNNLPDGIYVRVYEDRMDL 943
            FK FDI +D  DH++   GT  +  + + W K+IQ +W IL+ +LP+ I+VR YE RMDL
Sbjct: 305  FKRFDIVEDFSDHHYASKGTTSKQ-HSKDWAKRIQDEWRILEKDLPEMIFVRAYESRMDL 364

Query: 944  LRTVIVGAYGTPYQDGLFFFDFHLPPEYPDVPPSAYYHSGGWRINPNLYEEGKVCLSLLN 1003
            LR VI+GA GTPY DGLFFFD   P  YP  PP  +YHSGG RINPNLY  GKVCLSLL 
Sbjct: 365  LRAVIIGAQGTPYHDGLFFFDIFFPDTYPSTPPIVHYHSGGLRINPNLYNCGKVCLSLLG 424

Query: 1004 TWTGRGNEVWDPKSSSILQVLVSLQGLVLNSKPYFNEAGYDKQIGTAEGEKNSLSYNENT 1063
            TW+G   E W P +S++LQVLVS+QGL+LN KPYFNE GY++  G+A GE  S +Y+ENT
Sbjct: 425  TWSGNQREKWIPNTSTMLQVLVSIQGLILNQKPYFNEPGYERSAGSAHGESTSKAYSENT 484

Query: 1064 FLLNCKTIMYLMRKPPKDFEELVKEHFRRRGYYILKACDAYMKGHLIGSLTEDASIREES 1123
            F+L+ KT++Y MR+PPK FE+    HF    + +LKAC+AY  G   G L + A   EE+
Sbjct: 485  FILSLKTMVYTMRRPPKYFEDFAYGHFFSCAHDVLKACNAYRNGATPGYLVKGAPDVEEN 544

Query: 1124 DPNSTSVGFKLMLAKIV 1139
                +S+ F+  +A  V
Sbjct: 545  SAGMSSLKFRTDVATFV 560

BLAST of Carg05185-RA vs. TAIR10
Match: AT1G53025.1 (Ubiquitin-conjugating enzyme family protein)

HSP 1 Score: 281.6 bits (719), Expect = 2.2e-75
Identity = 142/302 (47.02%), Postives = 189/302 (62.58%), Query Frame = 0

Query: 849  DASEPSKMLVSAEASSNLRTAQSDGSACHEDGTCSFKGFDIAKDPLDHYFLGT-NGQTIN 908
            DAS  S   ++AE  S+++ + S+     E+    FK FD  +D  DH++         +
Sbjct: 210  DASASSSHPIAAEPISSVQDS-SNARKLKEEFLRDFKRFDTVEDFSDHHYASKGKSSKQH 269

Query: 909  GRKWLKKIQQDWTILQNNLPDGIYVRVYEDRMDLLRTVIVGAYGTPYQDGLFFFDFHLPP 968
             + W+KK+Q DW IL+N+LP+ I VR  E RMDLLR VI+GA GTPY DGLFFFD   P 
Sbjct: 270  SKNWVKKVQADWKILENDLPEAISVRACESRMDLLRAVIIGAEGTPYHDGLFFFDIQFPD 329

Query: 969  EYPDVPPSAYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSSILQVLVSLQG 1028
             YP VPP+ +YHSGG RINPNLY  GKVCLSLL TW G   E W P  S++LQ+LVS+Q 
Sbjct: 330  TYPSVPPNVHYHSGGLRINPNLYNCGKVCLSLLGTWAGSAREKWLPNESTMLQLLVSIQA 389

Query: 1029 LVLNSKPYFNEAGYDKQIGTAEGEKNSLSYNENTFLLNCKTIMYLMRKPPKDFEELVKEH 1088
            L+LN KPYFNE GY +  GTA GE  S  Y+EN FLL+ KT++Y +R+PP+ FEE V+ H
Sbjct: 390  LILNEKPYFNEPGYVQSAGTASGESKSKVYSENVFLLSLKTMVYSIRRPPQHFEEYVQNH 449

Query: 1089 FRRRGYYILKACDAYMKGHLIGSLTEDASIREESDPNSTSVGFKLMLAKIVPKLFSSLNE 1148
            +  R + I+KAC+AY  G  +GS+ +      E    S S  FK  +A  +  +     +
Sbjct: 450  YFVRSHDIVKACNAYKAGAPLGSMVKGGVQDLEEARQSGSKKFKTDVASFMQTVVDEFVK 509

Query: 1149 VG 1150
            +G
Sbjct: 510  LG 510

BLAST of Carg05185-RA vs. TAIR10
Match: AT1G53023.1 (Ubiquitin-conjugating enzyme family protein)

HSP 1 Score: 253.4 bits (646), Expect = 6.5e-67
Identity = 131/304 (43.09%), Postives = 182/304 (59.87%), Query Frame = 0

Query: 857  LVSAEASSNLRTAQSDGSACHEDGTCSFKGFDIAKDPLDHYFLGTNGQT--------ING 916
            +V  + SSN+R  +       E+    FK FD  +D  DH++                 G
Sbjct: 1    MVEIKDSSNVRKLK-------EEFLRDFKRFDTVEDFSDHHYAAEGSPAKLSLWHLKFKG 60

Query: 917  R--KWLKKIQQDWTILQNNLPDGIYVRVYEDRMDLLRTVIVGAYGTPYQDGLFFFDFHLP 976
            R   W+K IQ++W IL  NLP+ I+VR  E R+DLLR VI+GA GTPY DGLFFFD   P
Sbjct: 61   RPKNWVKDIQKEWKILDKNLPETIFVRACESRIDLLRAVIIGAEGTPYHDGLFFFDIQFP 120

Query: 977  PEYPDVPPSAYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSSILQVLVSLQ 1036
              YP VPP  +YHSGG RINPNLY+ GKVCLSL++TWTG+  E W PK S++LQ+LVS+Q
Sbjct: 121  DTYPSVPPKVHYHSGGLRINPNLYKCGKVCLSLISTWTGKKREKWLPKESTMLQLLVSIQ 180

Query: 1037 GLVLNSKPYFNEAGYDKQIGTAEGEKNSLSYNENTFLLNCKTIMYLMRKPPKDFEELVKE 1096
             L+LN KPY+NE GY+K +GT  GE  S  Y+EN F+ + KT+          FEE V+ 
Sbjct: 181  ALILNEKPYYNEPGYEKSMGTPLGESYSKDYSENVFVFSLKTM---------HFEEFVRS 240

Query: 1097 HFRRRGYYILKACDAYMKGHLIGSLTEDASIREESDPNSTSVGFKLMLAKIVPKLFSSLN 1151
            HF  R + I+KAC+AY  G  +GS+ +   +++++     S+ F++ +   +  +     
Sbjct: 241  HFFVRSHDIVKACNAYKDGAPVGSI-DKGGVKKQTRQRG-SLKFRINVTSFMKTVVDEFV 286

BLAST of Carg05185-RA vs. Swiss-Prot
Match: sp|Q9ZVX1|UBC23_ARATH (Probable ubiquitin-conjugating enzyme E2 23 OS=Arabidopsis thaliana OX=3702 GN=UBC23 PE=1 SV=1)

HSP 1 Score: 1225.7 bits (3170), Expect = 0.0e+00
Identity = 684/1137 (60.16%), Postives = 824/1137 (72.47%), Query Frame = 0

Query: 36   RDGVSTSGNIHMPTINSDEARRMNENRSDKHNIPHVYRRDIVKSKGSGMIGIVTEVAGDA 95
            +D   TS N+ + +   D    ++   S+    P++YR+DIVK+K +G +G+V+EVAGD 
Sbjct: 5    QDDPGTSTNVGVDSSVDDSMASLSICDSEH---PNIYRQDIVKNKKTGSVGVVSEVAGDX 64

Query: 96   DSDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQP 155
               XXXXXXXXXXXXXXXXXXXXXXXXX                                
Sbjct: 65   XXXXXXXXXXXXXXXXXXXXXXXXXXXXGKKASEENVVNGDGEKKADGNYKCG------A 124

Query: 156  LPADEVRVLWMDESETTQNVNDLTVIDRGFLHGDFVAAVSNPTGQAGVVVDVNISVDLLV 215
            L  D++RVLWMD +E  Q++ND+TVIDRGFLHGD+VA+ S PTGQ GVVVDVNISVDLL 
Sbjct: 125  LEGDQIRVLWMDNTEPVQDINDVTVIDRGFLHGDYVASASEPTGQVGVVVDVNISVDLLA 184

Query: 216  PDGSVVKNISSKDLIRVRDFAVGDYVVLGPWLGRIDDVLDNVTVMFDDGSKCKVTKAEPS 275
            PDGS+ K+IS+K+L RVRDFAVGDYVV GPWLGRIDDVLDNVTV+FDDGS CKV + EP 
Sbjct: 185  PDGSIHKDISTKNLKRVRDFAVGDYVVHGPWLGRIDDVLDNVTVLFDDGSMCKVLRVEPL 244

Query: 276  RLKPVSKNIL-EDASFPYYPGQRVRASSSTVFKNSKWLSGLWKPNRLQGTVTKVTVGSVF 335
            RLKP+ KN L EDA+FPYYPGQRV+ASSS+VFKNS+WLSGLWKPNRL+GTVTKVT GS+F
Sbjct: 245  RLKPIPKNNLEEDANFPYYPGQRVKASSSSVFKNSRWLSGLWKPNRLEGTVTKVTAGSIF 304

Query: 336  IYWIASAGYGPDSSTTPAEEQSPKNLRLLTCFSHVNWQLGDWCLLPSSFSSGST---KDL 395
            +YWIASAG+GPDSS +P EEQ+P NL LL+CF+H NWQ+GDWCLLPS   S +    K +
Sbjct: 305  VYWIASAGFGPDSSVSPPEEQNPSNLTLLSCFTHANWQVGDWCLLPSLNQSATIPLHKHV 364

Query: 396  SQTELSDSVTNTLDYAQTVGACDSEDTVVDELSGRTESTDLDSISACNGNYRDPECNILP 455
            S+  L DS     D  Q +G  D ED V DE+SG+ E   + + +       DP     P
Sbjct: 365  SKLRLYDS---QADRQQKIGR-DLED-VQDEVSGKVEPAGITAEALPKVTSDDP-----P 424

Query: 456  ESSSSRATKENTHETWPLHRKKIRKVVIRRDKXXXXXXXNFERALLIINTTTKVDVVWQD 515
            + + S  +KE  HE WPLHRKKIRK+VIR+DK       +FE+ALL++N+ T+VDV WQD
Sbjct: 425  QRNPS-VSKEPVHEPWPLHRKKIRKLVIRKDKKVKKKEESFEQALLVVNSRTRVDVSWQD 484

Query: 516  GRTELALDSTGLIPI-NPGDHEFVPEQYVVEKATDNDDDVTESRRVGVVKSVHAKERTAC 575
            G  E   ++  LIPI  PGDHEFV EQYVVEK +D+ D+ TE RR GVVK+V+AK+RTA 
Sbjct: 485  GTIECRREAITLIPIETPGDHEFVSEQYVVEKTSDDGDNTTEPRRAGVVKNVNAKDRTAS 544

Query: 576  VRWLKPVSRAEDPREFDIEEVVSVYELEGHPDYDYCYGDVVVRLSPVSDSIEALSSENNT 635
            VRWL P+ RAE+PREF+ EE+VSVYELEGHPDYDYCYGDVVVRLSP++ ++ A S  N+ 
Sbjct: 545  VRWLNPLRRAEEPREFEKEEIVSVYELEGHPDYDYCYGDVVVRLSPIAVALPASSPGNSF 604

Query: 636  EELNQQSSTNGVMSCPEKVSGSLKIEYPSSSDDCFDFSDLSWVGNITNLKNGDIEVFWAN 695
            EE  QQ   NG           + ++    ++   D S LSWVGNIT LK+GDIEV WA+
Sbjct: 605  EEATQQD--NGYQDSESHQEAKILVD-KEENEPSTDLSKLSWVGNITGLKDGDIEVTWAD 664

Query: 696  GMVSTVGPQAIYVVGRDDDDESTGAGSEISNGAASWETVXXXXXXMDVENAKEDIELQDT 755
            G +STVGP A+YVVGRDDDDES    SE S+ AASWET+       D +    +I  +D 
Sbjct: 665  GTISTVGPHAVYVVGRDDDDESVAGESETSD-AASWETLN------DDDRGAPEIPEEDL 724

Query: 756  GVDSEEEESE------QHNSGRSLALSVPLAAFRFVTRLATGIFSRGPRNRESMDLDSHS 815
            G  S  E +       +++SGR+ AL++PLAA  FVTRLA+GIFSR  ++     +DS S
Sbjct: 725  GRSSSIEGNSDADIYAENDSGRNGALALPLAAIEFVTRLASGIFSRARKS-----VDSSS 784

Query: 816  ESDTQSLEIQSLEEIDSGLQSSSLKSNSFTTSDMNSDCGRGEDDDASEPSKMLVSAEASS 875
               T     +  E  +   ++ SL   S +  ++  +C   E       +K ++S E S+
Sbjct: 785  SDYTVENVYKQAESTNPSDETDSLDDPSPSKVNVTDNC---ESKGTQANAKNILSGETST 844

Query: 876  NLRTAQSDGSACHEDGTCSFKGFDIAKDPLDHYFLGTNGQTINGRKWLKKIQQDWTILQN 935
             L     D     E  +CSF+ FDI++DPLDH+FLG +GQ    R+W KK+ QDW ILQN
Sbjct: 845  FLE--DEDKPVPSEGDSCSFRRFDISQDPLDHHFLGVDGQKTKERQWFKKVDQDWKILQN 904

Query: 936  NLPDGIYVRVYEDRMDLLRTVIVGAYGTPYQDGLFFFDFHLPPEYPDVPPSAYYHSGGWR 995
            NLPDGI+VR YEDRMDLLR VIVGA+GTPYQDGLFFFDFHLP +YP VPPSAYYHSGGWR
Sbjct: 905  NLPDGIFVRAYEDRMDLLRAVIVGAFGTPYQDGLFFFDFHLPSDYPSVPPSAYYHSGGWR 964

Query: 996  INPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSSILQVLVSLQGLVLNSKPYFNEAGYDKQ 1055
            +NPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSSILQVLVSLQGLVLNSKPYFNEAGYDKQ
Sbjct: 965  LNPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSSILQVLVSLQGLVLNSKPYFNEAGYDKQ 1024

Query: 1056 IGTAEGEKNSLSYNENTFLLNCKTIMYLMRKPPKDFEELVKEHFRRRGYYILKACDAYMK 1115
            +GTAEGEKNSL YNENTFLLNCKT+MYLMRKPPKDFEEL+K+HFR+RGYYILKACDAYMK
Sbjct: 1025 VGTAEGEKNSLGYNENTFLLNCKTMMYLMRKPPKDFEELIKDHFRKRGYYILKACDAYMK 1084

Query: 1116 GHLIGSLTEDAS-IREESDPNSTSVGFKLMLAKIVPKLFSSLNEVGADCRDFKHFQQ 1161
            G+LIGSLT+DAS I E S  NSTSVGFKLMLAKI PKLFS+L+EVGADC +F+H QQ
Sbjct: 1085 GYLIGSLTKDASVIDERSSANSTSVGFKLMLAKIAPKLFSALSEVGADCNEFQHLQQ 1101

BLAST of Carg05185-RA vs. Swiss-Prot
Match: sp|Q8VY10|UBC24_ARATH (Probable ubiquitin-conjugating enzyme E2 24 OS=Arabidopsis thaliana OX=3702 GN=UBC24 PE=1 SV=1)

HSP 1 Score: 592.8 bits (1527), Expect = 8.2e-168
Identity = 364/1010 (36.04%), Postives = 537/1010 (53.17%), Query Frame = 0

Query: 170  ETTQNVNDLTVIDRGFLHGDFVAAVSNPTGQAGVVVDVNISVDLLVPDGSVVKNISSKDL 229
            ET   +++    +RGF++GD V + + P+GQ+G V+++++ V+L    G ++K + +K L
Sbjct: 41   ETIGKIDEFLSFERGFMYGDIVRSATEPSGQSGRVINIDMFVNLESTHGKIMKEVDTKRL 100

Query: 230  IRVRDFAVGDYVVLGPWLGRIDDVLDNVTVMFDDGSKCKVTKAEPSRLKPVSKNILEDAS 289
             ++R  ++ DYV+ GPW+GR+D +++ V+V  DDG+  +V      +L  +  N+LED+ 
Sbjct: 101  QKLRSISLSDYVINGPWVGRVDKIVERVSVTLDDGTNYEVLVDGQDKLVAIPPNLLEDSQ 160

Query: 290  FPYYPGQRVRASSSTVFKNSKWLSGLWKPNRLQGTVTKVTVGSVFIYWIAS-AGYGPDSS 349
            + YYPGQRV+   +   +++ WL G W+  ++ GTV  V  G V++ W+AS    G  + 
Sbjct: 161  YSYYPGQRVQVKLAHAPRSTTWLCGTWRGTQVMGTVCTVEAGLVYVDWVASIVMEGDRNL 220

Query: 350  TTPAEEQSPKNLRLLTCFSHVNWQLGDWCLLPSSFSSGSTKDLSQTELSDSVTNTLDYAQ 409
            T P   Q+P++L LL C SH +WQLGDWC+LP S         S  ++++  T       
Sbjct: 221  TAPQALQNPESLTLLPCVSHASWQLGDWCILPGS---------SHCDIAERQT------- 280

Query: 410  TVGACDSEDTVVDELSGRTESTDLDSISACNGNYRDPECNILPESSSSRATKENTHETWP 469
                                     +++A N N    EC+   +   +R  + +      
Sbjct: 281  ------------------------PNVAAYNLN----ECHKTFQKGFNRNMQNS------ 340

Query: 470  LHRKKIRKVVIRRDKXXXXXXXNFERALLIINTTTKVDVVWQDGRTELALDSTGLIPINP 529
                                    +   +I  T  KV V+WQDG   L +DS  L+P+  
Sbjct: 341  ----------------------GLDELFVITKTKMKVAVMWQDGSCSLGVDSQQLLPVGA 400

Query: 530  -GDHEFVPEQYVVEKATDNDDDVTESRRVGVVKSVHAKERTACVRWLKPVSR-AEDPREF 589
               H+F PEQ+VVEK T N      S++ GVVK+V+AKE+T  V+W   V + A    + 
Sbjct: 401  VNAHDFWPEQFVVEKETCN------SKKWGVVKAVNAKEQTVKVQWTIQVEKEATGCVDE 460

Query: 590  DIEEVVSVYELEGHPDYDYCYGDVVVRL------SPVSDSIEALSSENNTEELNQQSSTN 649
             +EE+VS YEL  HPD+ +C+ DVVV+L       P +D+I A  +++    L  +S  +
Sbjct: 461  VMEEIVSAYELLEHPDFGFCFSDVVVKLLPEGKFDPNADTIVATEAKH----LLTESDYS 520

Query: 650  GVMSCPEKVSGSLKIEYPSSSDDCFDFSDLSWVGNITNLKNGDIEVFWANGMVSTVGPQA 709
            G                            LS +G +T  KNG ++V WANG  S V P  
Sbjct: 521  GAYF-------------------------LSSIGVVTGFKNGSVKVKWANGSTSKVAPCE 580

Query: 710  IYVVGRDDDDESTGAGSEISNGAASWETVXXXXXXMDVENAKEDIELQDTGVDSEEE--- 769
            I+ + R +   S+   SE S                 V++  + I   D    + +E   
Sbjct: 581  IWKMERSEYSNSSTVSSEGS-----------------VQDLSQKISQSDEASSNHQETGL 640

Query: 770  -------ESEQHNSGRSLALSVPLAAFRFVTRLATGIF----SRGPRNRESMDLDSHSES 829
                   ES   N     +  +P AA  F+T LA+ +F    S    +  S   DS  +S
Sbjct: 641  VKLYSVGESCNENIPECSSFFLPKAAIGFITNLASSLFGYQGSTSVISSHSRCNDSEDQS 700

Query: 830  DTQSLEIQSLEEIDSGLQSSSLKSNSFTTSDMNSDCGRGEDDDASEPSKMLVSAEA-SSN 889
            D++ L  ++ E  D                  NS+   GE D  +    + +  +  +  
Sbjct: 701  DSEVLVQETAESYD------------------NSETNSGEVDMTTTMVNIPIEGKGINKT 760

Query: 890  LRTAQSDGSACHEDGTCSFKGFDIAKDPLDHYFLGTN---GQTINGRKWLKKIQQDWTIL 949
            L +   + S         F+ FD+  D  DH+FL ++    Q+   + W+KK+QQ+W+ L
Sbjct: 761  LDSTLLENSR----NQVRFRQFDMVNDCSDHHFLSSDKGLAQSQVTKSWVKKVQQEWSNL 820

Query: 950  QNNLPDGIYVRVYEDRMDLLRTVIVGAYGTPYQDGLFFFDFHLPPEYPDVPPSAYYHSGG 1009
            + NLP+ IYVRV E+RMDLLR  +VGA GTPY DGLFFFD  LPP+YP  PP  +YHSGG
Sbjct: 821  EANLPNTIYVRVCEERMDLLRAALVGAPGTPYHDGLFFFDIMLPPQYPHEPPMVHYHSGG 880

Query: 1010 WRINPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSSILQVLVSLQGLVLNSKPYFNEAGYD 1069
             R+NPNLYE G+VCLSLLNTW+G G EVW+  SSSILQ+L+S Q LVLN KPYFNEAGYD
Sbjct: 881  MRLNPNLYESGRVCLSLLNTWSGSGTEVWNAGSSSILQLLLSFQALVLNEKPYFNEAGYD 899

Query: 1070 KQIGTAEGEKNSLSYNENTFLLNCKTIMYLMRKPPKDFEELVKEHFRRRGYYILKACDAY 1129
            KQ+G AEGEKNS+SYNEN FL+ CK+++ ++RKPPK FE LVK+HF  R  ++L AC AY
Sbjct: 941  KQLGRAEGEKNSVSYNENAFLITCKSMISMLRKPPKHFEMLVKDHFTHRAQHVLAACKAY 899

Query: 1130 MKGHLIGSLTEDASIREESDPNSTSVGFKLMLAKIVPKLFSSLNEVGADC 1153
            M+G  +GS    A+++  S  NST  GFK+ML+K+ PKL  + +E+G DC
Sbjct: 1001 MEGVPVGS---SANLQGNSTTNST--GFKIMLSKLYPKLLEAFSEIGVDC 899

BLAST of Carg05185-RA vs. Swiss-Prot
Match: sp|Q9LUQ5|UBC25_ARATH (Probable ubiquitin-conjugating enzyme E2 25 OS=Arabidopsis thaliana OX=3702 GN=UBC25 PE=2 SV=1)

HSP 1 Score: 285.0 bits (728), Expect = 3.7e-75
Identity = 135/257 (52.53%), Postives = 175/257 (68.09%), Query Frame = 0

Query: 884  FKGFDIAKDPLDHYFL--GTNGQTINGRKWLKKIQQDWTILQNNLPDGIYVRVYEDRMDL 943
            FK FDI +D  DH++   GT  +  + + W K+IQ +W IL+ +LP+ I+VR YE RMDL
Sbjct: 305  FKRFDIVEDFSDHHYASKGTTSKQ-HSKDWAKRIQDEWRILEKDLPEMIFVRAYESRMDL 364

Query: 944  LRTVIVGAYGTPYQDGLFFFDFHLPPEYPDVPPSAYYHSGGWRINPNLYEEGKVCLSLLN 1003
            LR VI+GA GTPY DGLFFFD   P  YP  PP  +YHSGG RINPNLY  GKVCLSLL 
Sbjct: 365  LRAVIIGAQGTPYHDGLFFFDIFFPDTYPSTPPIVHYHSGGLRINPNLYNCGKVCLSLLG 424

Query: 1004 TWTGRGNEVWDPKSSSILQVLVSLQGLVLNSKPYFNEAGYDKQIGTAEGEKNSLSYNENT 1063
            TW+G   E W P +S++LQVLVS+QGL+LN KPYFNE GY++  G+A GE  S +Y+ENT
Sbjct: 425  TWSGNQREKWIPNTSTMLQVLVSIQGLILNQKPYFNEPGYERSAGSAHGESTSKAYSENT 484

Query: 1064 FLLNCKTIMYLMRKPPKDFEELVKEHFRRRGYYILKACDAYMKGHLIGSLTEDASIREES 1123
            F+L+ KT++Y MR+PPK FE+    HF    + +LKAC+AY  G   G L + A   EE+
Sbjct: 485  FILSLKTMVYTMRRPPKYFEDFAYGHFFSCAHDVLKACNAYRNGATPGYLVKGAPDVEEN 544

Query: 1124 DPNSTSVGFKLMLAKIV 1139
                +S+ F+  +A  V
Sbjct: 545  SAGMSSLKFRTDVATFV 560

BLAST of Carg05185-RA vs. Swiss-Prot
Match: sp|Q8GY87|UBC26_ARATH (Probable ubiquitin-conjugating enzyme E2 26 OS=Arabidopsis thaliana OX=3702 GN=UBC26 PE=2 SV=2)

HSP 1 Score: 281.6 bits (719), Expect = 4.0e-74
Identity = 142/302 (47.02%), Postives = 189/302 (62.58%), Query Frame = 0

Query: 849  DASEPSKMLVSAEASSNLRTAQSDGSACHEDGTCSFKGFDIAKDPLDHYFLGT-NGQTIN 908
            DAS  S   ++AE  S+++ + S+     E+    FK FD  +D  DH++         +
Sbjct: 210  DASASSSHPIAAEPISSVQDS-SNARKLKEEFLRDFKRFDTVEDFSDHHYASKGKSSKQH 269

Query: 909  GRKWLKKIQQDWTILQNNLPDGIYVRVYEDRMDLLRTVIVGAYGTPYQDGLFFFDFHLPP 968
             + W+KK+Q DW IL+N+LP+ I VR  E RMDLLR VI+GA GTPY DGLFFFD   P 
Sbjct: 270  SKNWVKKVQADWKILENDLPEAISVRACESRMDLLRAVIIGAEGTPYHDGLFFFDIQFPD 329

Query: 969  EYPDVPPSAYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSSILQVLVSLQG 1028
             YP VPP+ +YHSGG RINPNLY  GKVCLSLL TW G   E W P  S++LQ+LVS+Q 
Sbjct: 330  TYPSVPPNVHYHSGGLRINPNLYNCGKVCLSLLGTWAGSAREKWLPNESTMLQLLVSIQA 389

Query: 1029 LVLNSKPYFNEAGYDKQIGTAEGEKNSLSYNENTFLLNCKTIMYLMRKPPKDFEELVKEH 1088
            L+LN KPYFNE GY +  GTA GE  S  Y+EN FLL+ KT++Y +R+PP+ FEE V+ H
Sbjct: 390  LILNEKPYFNEPGYVQSAGTASGESKSKVYSENVFLLSLKTMVYSIRRPPQHFEEYVQNH 449

Query: 1089 FRRRGYYILKACDAYMKGHLIGSLTEDASIREESDPNSTSVGFKLMLAKIVPKLFSSLNE 1148
            +  R + I+KAC+AY  G  +GS+ +      E    S S  FK  +A  +  +     +
Sbjct: 450  YFVRSHDIVKACNAYKAGAPLGSMVKGGVQDLEEARQSGSKKFKTDVASFMQTVVDEFVK 509

Query: 1149 VG 1150
            +G
Sbjct: 510  LG 510

BLAST of Carg05185-RA vs. Swiss-Prot
Match: sp|Q9C918|UBC38_ARATH (Putative ubiquitin-conjugating enzyme E2 38 OS=Arabidopsis thaliana OX=3702 GN=UBC38 PE=3 SV=2)

HSP 1 Score: 276.6 bits (706), Expect = 1.3e-72
Identity = 135/268 (50.37%), Postives = 172/268 (64.18%), Query Frame = 0

Query: 883  SFKGFDIAKDPLDHYFLGT-NGQTINGRKWLKKIQQDWTILQNNLPDGIYVRVYEDRMDL 942
            +FK FD  +D  DH+++        + + W+KK+Q +W IL  NLP+ I+VR  E RMDL
Sbjct: 26   NFKRFDSVEDFSDHHYVSKGKASKQHSKNWVKKVQDEWKILNQNLPETIFVRACESRMDL 85

Query: 943  LRTVIVGAYGTPYQDGLFFFDFHLPPEYPDVPPSAYYHSGGWRINPNLYEEGKVCLSLLN 1002
            LR VI+GA GTPY DGLFFFD   P  YP VPP+ YYHSGG RINPNLY  GKVCLSLL 
Sbjct: 86   LRAVIIGAEGTPYHDGLFFFDIQFPDTYPSVPPNVYYHSGGLRINPNLYNCGKVCLSLLG 145

Query: 1003 TWTGRGNEVWDPKSSSILQVLVSLQGLVLNSKPYFNEAGYDKQIGTAEGEKNSLSYNENT 1062
            TW G   + W PK S++LQVLVS+Q LVLN +PYFNE GY    GT  GE  S  Y+EN 
Sbjct: 146  TWHGNARQSWLPKESTMLQVLVSIQALVLNEQPYFNEPGYGLIKGTWLGESKSKVYSENV 205

Query: 1063 FLLNCKTIMYLMRKPPKDFEELVKEHFRRRGYYILKACDAYMKGHLIGSLTEDASIREES 1122
            FLL+ KT++Y MRKPP+ FEE V+ H+  R + I+KAC+AY  G  +GS+ +      E 
Sbjct: 206  FLLSLKTMVYSMRKPPQHFEEYVQNHYFVRSHDIVKACNAYKAGAPLGSMVKGGVQDLEQ 265

Query: 1123 DPNSTSVGFKLMLAKIVPKLFSSLNEVG 1150
               S S  FK  +A  +  +     ++G
Sbjct: 266  ARQSGSKKFKTDVASFMQTVVDEFVKLG 293

BLAST of Carg05185-RA vs. TrEMBL
Match: tr|A0A0A0LLP6|A0A0A0LLP6_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G270870 PE=4 SV=1)

HSP 1 Score: 1835.8 bits (4754), Expect = 0.0e+00
Identity = 985/1150 (85.65%), Postives = 1036/1150 (90.09%), Query Frame = 0

Query: 15   MGQQDSVDEESLTDTCDSYMNRDGVSTSGNIHMPTINSDEARRMNENRSDKHNIPHVYRR 74
            M QQDSVD+ESL  TC+   NRDGVS+S +I + TIN+DEAR  NEN SDK NIPH+YR+
Sbjct: 2    MEQQDSVDKESLIGTCN---NRDGVSSS-DIQITTINNDEARIKNENTSDKPNIPHIYRQ 61

Query: 75   DIVKSKGSGMIGIVTEVAGDADSDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 134
            DIVKSKGSGMIGIVTEVAGD    XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 62   DIVKSKGSGMIGIVTEVAGDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 121

Query: 135  XXXXXXXXXXXXXXXXXXXQPLPADEVRVLWMDESETTQNVNDLTVIDRGFLHGDFVAAV 194
            XXXXXX             QPLP +EVRVLWMDESETTQ+VNDLTVIDRGF+HGDFVAAV
Sbjct: 122  XXXXXXGNGRHSNGDNYKSQPLPDNEVRVLWMDESETTQHVNDLTVIDRGFVHGDFVAAV 181

Query: 195  SNPTGQAGVVVDVNISVDLLVPDGSVVKNISSKDLIRVRDFAVGDYVVLGPWLGRIDDVL 254
            S+PTGQAGVVVDVNISVDLLVPDGS++K+ISSKDL RVRDF VGDYVVLGPWLGR+DDVL
Sbjct: 182  SDPTGQAGVVVDVNISVDLLVPDGSIMKDISSKDLKRVRDFTVGDYVVLGPWLGRVDDVL 241

Query: 255  DNVTVMFDDGSKCKVTKAEPSRLKPVSKNILEDASFPYYPGQRVRASSSTVFKNSKWLSG 314
            DNVTVMFDDGSKCKVTKAEP RLKPVSKN LEDA+FPYYPGQRVRA +STVFKNSKWLSG
Sbjct: 242  DNVTVMFDDGSKCKVTKAEPLRLKPVSKNTLEDANFPYYPGQRVRA-TSTVFKNSKWLSG 301

Query: 315  LWKPNRLQGTVTKVTVGSVFIYWIASAGYGPDSSTTPAEEQSPKNLRLLTCFSHVNWQLG 374
            LWKPNRL+GTVTKVTVGSVFIYWIASAGYGPDSST PAEEQ+PKNLRLLTCFSH NWQLG
Sbjct: 302  LWKPNRLEGTVTKVTVGSVFIYWIASAGYGPDSSTAPAEEQTPKNLRLLTCFSHANWQLG 361

Query: 375  DWCLLPSSFSSGSTKDLSQTELSDSVTNTLDYAQTVGACDSEDTVVDELSGRTESTDLDS 434
            DWCLLP SFS+G TKD SQTEL  SVTNTLD AQ+VGACDSEDTV+DELSG TESTDLDS
Sbjct: 362  DWCLLPPSFSAGLTKDPSQTEL--SVTNTLDCAQSVGACDSEDTVLDELSGTTESTDLDS 421

Query: 435  ISACNGNYRDPECNILPESSSSRATKENTHETWPLHRKKIRKVVIRRDKXXXXXXXNFER 494
            ISAC+GNYR+P  N LPESSSSRA KE  HETWPLHRKKIRKVV+RRDK       NFER
Sbjct: 422  ISACDGNYRNPVDNSLPESSSSRALKETAHETWPLHRKKIRKVVVRRDKKARKKEENFER 481

Query: 495  ALLIINTTTKVDVVWQDGRTELALDSTGLIPI-NPGDHEFVPEQYVVEKATDNDDDVTES 554
            ALLIINT T+VDV WQDG+TEL LDST LIPI NPGDHEFVPEQYVVEKA+DNDDDV+ES
Sbjct: 482  ALLIINTKTRVDVAWQDGQTELGLDSTSLIPIDNPGDHEFVPEQYVVEKASDNDDDVSES 541

Query: 555  RRVGVVKSVHAKERTACVRWLKPVSRAEDPREFDIEEVVSVYELEGHPDYDYCYGDVVVR 614
            RRVGVVKSVHAKERTACVRWLKPVSRAEDPREFD EE+VSVYELEGHPDYDYCYGDVVVR
Sbjct: 542  RRVGVVKSVHAKERTACVRWLKPVSRAEDPREFDKEEIVSVYELEGHPDYDYCYGDVVVR 601

Query: 615  LSPVSDSIEALSSENNTEELNQQSSTNGVMSCPE--KVSGSLKIEYPSSSDDCFDFSDLS 674
            LSPVSDS EA+S   NTEEL QQSSTN +MSC E    SGS KIE  S SDDC DFSDLS
Sbjct: 602  LSPVSDSAEAMSLGINTEELKQQSSTNEMMSCTEFNNASGSQKIEDTSCSDDCIDFSDLS 661

Query: 675  WVGNITNLKNGDIEVFWANGMVSTVGPQAIYVVGRDDDDESTGAGSEISNGAASWETVXX 734
            WVGNIT LKNGDIEV WANGMVSTVGPQAIYVVGRDDDDES  AGSE+SNGAASWETV  
Sbjct: 662  WVGNITGLKNGDIEVTWANGMVSTVGPQAIYVVGRDDDDESIAAGSEVSNGAASWETVDN 721

Query: 735  XXXXMDVENAKEDIELQDTGVDSEEEESEQHNSGRSLALSVPLAAFRFVTRLATGIFSRG 794
                  VENA EDIELQDTG +SEEEESEQ NSGR+LALSVPLAA RFVTRLA GIFSRG
Sbjct: 722  DEMD-SVENAAEDIELQDTGANSEEEESEQSNSGRNLALSVPLAALRFVTRLAAGIFSRG 781

Query: 795  PRNRESMDLDSHSESDTQSLEIQSLEEIDSGLQSSSLKSNSFTTSDMNSDCGRGEDDDAS 854
            PRN +SMDLDSHSES+ QSL+IQ+ E  DSGLQS+SLKSNSF  SDMNSDCGRGED  AS
Sbjct: 782  PRNPDSMDLDSHSESEIQSLDIQASEGKDSGLQSTSLKSNSFDASDMNSDCGRGEDGVAS 841

Query: 855  EPSKMLVSAEASSNLRTAQSDGSACHEDGTCSFKGFDIAKDPLDHYFLGTNGQTINGRKW 914
            EPS++L SA+ SSNLRT + D SACHEDGTCSFKGFDIAKDPLDHYFLGTNGQT NGRKW
Sbjct: 842  EPSEVLESAKTSSNLRTVELDASACHEDGTCSFKGFDIAKDPLDHYFLGTNGQTNNGRKW 901

Query: 915  LKKIQQDWTILQNNLPDGIYVRVYEDRMDLLRTVIVGAYGTPYQDGLFFFDFHLPPEYPD 974
            LKKIQQDW+ILQNNLPDGIYVRVYEDRMDLLR VIVGAYGTPYQDGLFFFDFHLPPEYPD
Sbjct: 902  LKKIQQDWSILQNNLPDGIYVRVYEDRMDLLRAVIVGAYGTPYQDGLFFFDFHLPPEYPD 961

Query: 975  VPPSAYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSSILQVLVSLQGLVLN 1034
            VPPSAYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSSILQVLVSLQGLVLN
Sbjct: 962  VPPSAYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSSILQVLVSLQGLVLN 1021

Query: 1035 SKPYFNEAGYDKQIGTAEGEKNSLSYNENTFLLNCKTIMYLMRKPPKDFEELVKEHFRRR 1094
            SKPYFNEAGYDKQ+GTAEGEKNSLSYNENTFLLNCKTIMYLMRKPPKDFEEL+KEHFRRR
Sbjct: 1022 SKPYFNEAGYDKQVGTAEGEKNSLSYNENTFLLNCKTIMYLMRKPPKDFEELIKEHFRRR 1081

Query: 1095 GYYILKACDAYMKGHLIGSLTEDASIREESDPNSTSVGFKLMLAKIVPKLFSSLNEVGAD 1154
            GY+ILKACDAYMKGHLIGSLTEDAS+R ESDPNSTSVGFKLMLAKIVPKLFSSLNEVGAD
Sbjct: 1082 GYFILKACDAYMKGHLIGSLTEDASVRVESDPNSTSVGFKLMLAKIVPKLFSSLNEVGAD 1141

Query: 1155 CRDFKHFQQL 1162
            C+DFKHFQQL
Sbjct: 1142 CQDFKHFQQL 1143

BLAST of Carg05185-RA vs. TrEMBL
Match: tr|A0A1S3C7G0|A0A1S3C7G0_CUCME (probable ubiquitin-conjugating enzyme E2 23 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103497343 PE=4 SV=1)

HSP 1 Score: 1825.8 bits (4728), Expect = 0.0e+00
Identity = 973/1148 (84.76%), Postives = 1025/1148 (89.29%), Query Frame = 0

Query: 15   MGQQDSVDEESLTDTCDSYMNRDGVSTSGNIHMPTINSDEARRMNENRSDKHNIPHVYRR 74
            M QQDSVD+ESL  TC+   NRDGVS+S +I + T N+DE RR NEN SDK NIPH+YR+
Sbjct: 1    MEQQDSVDKESLIGTCN---NRDGVSSS-DIQITTKNNDETRRKNENTSDKPNIPHIYRQ 60

Query: 75   DIVKSKGSGMIGIVTEVAGDADSDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 134
            DIVKSKGSGMIGIVTEVAGD    XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 61   DIVKSKGSGMIGIVTEVAGDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 120

Query: 135  XXXXXXXXXXXXXXXXXXXQPLPADEVRVLWMDESETTQNVNDLTVIDRGFLHGDFVAAV 194
            X                  QPLP DEVRVLWMDESETTQ+VNDLTVIDRGF+HGDFVAAV
Sbjct: 121  XNCSDDGNVRHSNGDNYKSQPLPDDEVRVLWMDESETTQHVNDLTVIDRGFVHGDFVAAV 180

Query: 195  SNPTGQAGVVVDVNISVDLLVPDGSVVKNISSKDLIRVRDFAVGDYVVLGPWLGRIDDVL 254
            S+PTGQAGVVVDVNISVDLLVPDGS++K+ISSKDL RVR+F VGDYVVLGPWLGR+DDVL
Sbjct: 181  SDPTGQAGVVVDVNISVDLLVPDGSIMKDISSKDLKRVREFTVGDYVVLGPWLGRVDDVL 240

Query: 255  DNVTVMFDDGSKCKVTKAEPSRLKPVSKNILEDASFPYYPGQRVRASSSTVFKNSKWLSG 314
            DNVTVMFDDGSKCKVTKAEP RLKPVSKN LEDA+FPYYPGQRVRA +STVFKNSKWLSG
Sbjct: 241  DNVTVMFDDGSKCKVTKAEPLRLKPVSKNTLEDANFPYYPGQRVRA-TSTVFKNSKWLSG 300

Query: 315  LWKPNRLQGTVTKVTVGSVFIYWIASAGYGPDSSTTPAEEQSPKNLRLLTCFSHVNWQLG 374
            LWKPNRL+GTVTKVTVGSVFIYWIASAGYGPDSSTTPAEEQ+PKNL+LLTCFSH NWQLG
Sbjct: 301  LWKPNRLEGTVTKVTVGSVFIYWIASAGYGPDSSTTPAEEQTPKNLKLLTCFSHANWQLG 360

Query: 375  DWCLLPSSFSSGSTKDLSQTELSDSVTNTLDYAQTVGACDSEDTVVDELSGRTESTDLDS 434
            DWCLLP SFS+G TKD S TEL  SVTNTL+ AQ+VGACDSEDTV+DELSG TESTDLDS
Sbjct: 361  DWCLLPPSFSAGLTKDPSHTEL--SVTNTLECAQSVGACDSEDTVLDELSGTTESTDLDS 420

Query: 435  ISACNGNYRDPECNILPESSSSRATKENTHETWPLHRKKIRKVVIRRDKXXXXXXXNFER 494
            ISAC+GNYR+P  N LPESSSSRA KE  HETWPLHRKKIRKVV+RRDK       NFER
Sbjct: 421  ISACDGNYRNPVYNSLPESSSSRALKETAHETWPLHRKKIRKVVVRRDKKARKKEENFER 480

Query: 495  ALLIINTTTKVDVVWQDGRTELALDSTGLIPI-NPGDHEFVPEQYVVEKATDNDDDVTES 554
            ALLIINT TKVDV WQDG+TEL LDST LIPI NPGDHEFVPEQYVVEKA+DNDDDV+ES
Sbjct: 481  ALLIINTKTKVDVAWQDGQTELGLDSTSLIPIDNPGDHEFVPEQYVVEKASDNDDDVSES 540

Query: 555  RRVGVVKSVHAKERTACVRWLKPVSRAEDPREFDIEEVVSVYELEGHPDYDYCYGDVVVR 614
            RRVGVVKSVHAKERTACVRWLKPVSRAEDPREFD EE+VSVYELEGHPDYDYCYGDVVVR
Sbjct: 541  RRVGVVKSVHAKERTACVRWLKPVSRAEDPREFDKEEIVSVYELEGHPDYDYCYGDVVVR 600

Query: 615  LSPVSDSIEALSSENNTEELNQQSSTNGVMSCPEKVSGSLKIEYPSSSDDCFDFSDLSWV 674
            LSPVSDS EA+S   NTEEL QQSST+ +MSC E VSGS KIE  S SDDC DFSDLSWV
Sbjct: 601  LSPVSDSAEAMSLGINTEELKQQSSTDEMMSCTENVSGSQKIEDTSCSDDCIDFSDLSWV 660

Query: 675  GNITNLKNGDIEVFWANGMVSTVGPQAIYVVGRDDDDESTGAGSEISNGAASWETVXXXX 734
            GNIT LKNGDIEV WANGMVSTVGPQAIYVVGRDDDDES  AGSE+SNGAASWETV    
Sbjct: 661  GNITGLKNGDIEVTWANGMVSTVGPQAIYVVGRDDDDESIAAGSEVSNGAASWETVDNDE 720

Query: 735  XXMDVENAKEDIELQDTGVDSEEEESEQHNSGRSLALSVPLAAFRFVTRLATGIFSRGPR 794
                VENA EDIELQDTG +SEEEESEQ NSGR+LALSVPLAA RFVTRLA GIFSRGPR
Sbjct: 721  MD-SVENAAEDIELQDTGANSEEEESEQSNSGRNLALSVPLAALRFVTRLAAGIFSRGPR 780

Query: 795  NRESMDLDSHSESDTQSLEIQSLEEIDSGLQSSSLKSNSFTTSDMNSDCGRGEDDDASEP 854
            N +SMDLDSH        +IQ+ EE DSGLQS+SLKSNSF  SDMNSDCGRGED+ ASEP
Sbjct: 781  NPDSMDLDSHXXXXXXXXDIQASEEKDSGLQSTSLKSNSFDASDMNSDCGRGEDNVASEP 840

Query: 855  SKMLVSAEASSNLRTAQSDGSACHEDGTCSFKGFDIAKDPLDHYFLGTNGQTINGRKWLK 914
            S++L SAE SSNLRT + D SA HEDGTCSFKGFDIAKDPLDHYFLGTNGQT NGRKWLK
Sbjct: 841  SEVLESAETSSNLRTVELDASASHEDGTCSFKGFDIAKDPLDHYFLGTNGQTNNGRKWLK 900

Query: 915  KIQQDWTILQNNLPDGIYVRVYEDRMDLLRTVIVGAYGTPYQDGLFFFDFHLPPEYPDVP 974
            KIQQDW+ILQNNLPDGIYVRVYEDRMDLLR VIVGAYGTPYQDGLFFFDFHLPPEYPDVP
Sbjct: 901  KIQQDWSILQNNLPDGIYVRVYEDRMDLLRAVIVGAYGTPYQDGLFFFDFHLPPEYPDVP 960

Query: 975  PSAYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSSILQVLVSLQGLVLNSK 1034
            PSAYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSSILQVLVSLQGLVLNSK
Sbjct: 961  PSAYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSSILQVLVSLQGLVLNSK 1020

Query: 1035 PYFNEAGYDKQIGTAEGEKNSLSYNENTFLLNCKTIMYLMRKPPKDFEELVKEHFRRRGY 1094
            PYFNEAGYDKQ+GTAEGEKNSLSYNENTFLLNCKTIMYLMRKPPKDFEEL+KEHFR+RGY
Sbjct: 1021 PYFNEAGYDKQVGTAEGEKNSLSYNENTFLLNCKTIMYLMRKPPKDFEELIKEHFRKRGY 1080

Query: 1095 YILKACDAYMKGHLIGSLTEDASIREESDPNSTSVGFKLMLAKIVPKLFSSLNEVGADCR 1154
            +ILKACDAYMKGHLIGSLTEDASIREESDPNSTSVGFKLMLAKIVPKLFSSLNEVGADC+
Sbjct: 1081 FILKACDAYMKGHLIGSLTEDASIREESDPNSTSVGFKLMLAKIVPKLFSSLNEVGADCQ 1140

Query: 1155 DFKHFQQL 1162
            DF HFQQL
Sbjct: 1141 DFMHFQQL 1140

BLAST of Carg05185-RA vs. TrEMBL
Match: tr|A0A1S3C6T8|A0A1S3C6T8_CUCME (probable ubiquitin-conjugating enzyme E2 23 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103497343 PE=4 SV=1)

HSP 1 Score: 1825.8 bits (4728), Expect = 0.0e+00
Identity = 973/1148 (84.76%), Postives = 1025/1148 (89.29%), Query Frame = 0

Query: 15   MGQQDSVDEESLTDTCDSYMNRDGVSTSGNIHMPTINSDEARRMNENRSDKHNIPHVYRR 74
            M QQDSVD+ESL  TC+   NRDGVS+S +I + T N+DE RR NEN SDK NIPH+YR+
Sbjct: 2    MEQQDSVDKESLIGTCN---NRDGVSSS-DIQITTKNNDETRRKNENTSDKPNIPHIYRQ 61

Query: 75   DIVKSKGSGMIGIVTEVAGDADSDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 134
            DIVKSKGSGMIGIVTEVAGD    XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 62   DIVKSKGSGMIGIVTEVAGDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 121

Query: 135  XXXXXXXXXXXXXXXXXXXQPLPADEVRVLWMDESETTQNVNDLTVIDRGFLHGDFVAAV 194
            X                  QPLP DEVRVLWMDESETTQ+VNDLTVIDRGF+HGDFVAAV
Sbjct: 122  XNCSDDGNVRHSNGDNYKSQPLPDDEVRVLWMDESETTQHVNDLTVIDRGFVHGDFVAAV 181

Query: 195  SNPTGQAGVVVDVNISVDLLVPDGSVVKNISSKDLIRVRDFAVGDYVVLGPWLGRIDDVL 254
            S+PTGQAGVVVDVNISVDLLVPDGS++K+ISSKDL RVR+F VGDYVVLGPWLGR+DDVL
Sbjct: 182  SDPTGQAGVVVDVNISVDLLVPDGSIMKDISSKDLKRVREFTVGDYVVLGPWLGRVDDVL 241

Query: 255  DNVTVMFDDGSKCKVTKAEPSRLKPVSKNILEDASFPYYPGQRVRASSSTVFKNSKWLSG 314
            DNVTVMFDDGSKCKVTKAEP RLKPVSKN LEDA+FPYYPGQRVRA +STVFKNSKWLSG
Sbjct: 242  DNVTVMFDDGSKCKVTKAEPLRLKPVSKNTLEDANFPYYPGQRVRA-TSTVFKNSKWLSG 301

Query: 315  LWKPNRLQGTVTKVTVGSVFIYWIASAGYGPDSSTTPAEEQSPKNLRLLTCFSHVNWQLG 374
            LWKPNRL+GTVTKVTVGSVFIYWIASAGYGPDSSTTPAEEQ+PKNL+LLTCFSH NWQLG
Sbjct: 302  LWKPNRLEGTVTKVTVGSVFIYWIASAGYGPDSSTTPAEEQTPKNLKLLTCFSHANWQLG 361

Query: 375  DWCLLPSSFSSGSTKDLSQTELSDSVTNTLDYAQTVGACDSEDTVVDELSGRTESTDLDS 434
            DWCLLP SFS+G TKD S TEL  SVTNTL+ AQ+VGACDSEDTV+DELSG TESTDLDS
Sbjct: 362  DWCLLPPSFSAGLTKDPSHTEL--SVTNTLECAQSVGACDSEDTVLDELSGTTESTDLDS 421

Query: 435  ISACNGNYRDPECNILPESSSSRATKENTHETWPLHRKKIRKVVIRRDKXXXXXXXNFER 494
            ISAC+GNYR+P  N LPESSSSRA KE  HETWPLHRKKIRKVV+RRDK       NFER
Sbjct: 422  ISACDGNYRNPVYNSLPESSSSRALKETAHETWPLHRKKIRKVVVRRDKKARKKEENFER 481

Query: 495  ALLIINTTTKVDVVWQDGRTELALDSTGLIPI-NPGDHEFVPEQYVVEKATDNDDDVTES 554
            ALLIINT TKVDV WQDG+TEL LDST LIPI NPGDHEFVPEQYVVEKA+DNDDDV+ES
Sbjct: 482  ALLIINTKTKVDVAWQDGQTELGLDSTSLIPIDNPGDHEFVPEQYVVEKASDNDDDVSES 541

Query: 555  RRVGVVKSVHAKERTACVRWLKPVSRAEDPREFDIEEVVSVYELEGHPDYDYCYGDVVVR 614
            RRVGVVKSVHAKERTACVRWLKPVSRAEDPREFD EE+VSVYELEGHPDYDYCYGDVVVR
Sbjct: 542  RRVGVVKSVHAKERTACVRWLKPVSRAEDPREFDKEEIVSVYELEGHPDYDYCYGDVVVR 601

Query: 615  LSPVSDSIEALSSENNTEELNQQSSTNGVMSCPEKVSGSLKIEYPSSSDDCFDFSDLSWV 674
            LSPVSDS EA+S   NTEEL QQSST+ +MSC E VSGS KIE  S SDDC DFSDLSWV
Sbjct: 602  LSPVSDSAEAMSLGINTEELKQQSSTDEMMSCTENVSGSQKIEDTSCSDDCIDFSDLSWV 661

Query: 675  GNITNLKNGDIEVFWANGMVSTVGPQAIYVVGRDDDDESTGAGSEISNGAASWETVXXXX 734
            GNIT LKNGDIEV WANGMVSTVGPQAIYVVGRDDDDES  AGSE+SNGAASWETV    
Sbjct: 662  GNITGLKNGDIEVTWANGMVSTVGPQAIYVVGRDDDDESIAAGSEVSNGAASWETVDNDE 721

Query: 735  XXMDVENAKEDIELQDTGVDSEEEESEQHNSGRSLALSVPLAAFRFVTRLATGIFSRGPR 794
                VENA EDIELQDTG +SEEEESEQ NSGR+LALSVPLAA RFVTRLA GIFSRGPR
Sbjct: 722  MD-SVENAAEDIELQDTGANSEEEESEQSNSGRNLALSVPLAALRFVTRLAAGIFSRGPR 781

Query: 795  NRESMDLDSHSESDTQSLEIQSLEEIDSGLQSSSLKSNSFTTSDMNSDCGRGEDDDASEP 854
            N +SMDLDSH        +IQ+ EE DSGLQS+SLKSNSF  SDMNSDCGRGED+ ASEP
Sbjct: 782  NPDSMDLDSHXXXXXXXXDIQASEEKDSGLQSTSLKSNSFDASDMNSDCGRGEDNVASEP 841

Query: 855  SKMLVSAEASSNLRTAQSDGSACHEDGTCSFKGFDIAKDPLDHYFLGTNGQTINGRKWLK 914
            S++L SAE SSNLRT + D SA HEDGTCSFKGFDIAKDPLDHYFLGTNGQT NGRKWLK
Sbjct: 842  SEVLESAETSSNLRTVELDASASHEDGTCSFKGFDIAKDPLDHYFLGTNGQTNNGRKWLK 901

Query: 915  KIQQDWTILQNNLPDGIYVRVYEDRMDLLRTVIVGAYGTPYQDGLFFFDFHLPPEYPDVP 974
            KIQQDW+ILQNNLPDGIYVRVYEDRMDLLR VIVGAYGTPYQDGLFFFDFHLPPEYPDVP
Sbjct: 902  KIQQDWSILQNNLPDGIYVRVYEDRMDLLRAVIVGAYGTPYQDGLFFFDFHLPPEYPDVP 961

Query: 975  PSAYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSSILQVLVSLQGLVLNSK 1034
            PSAYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSSILQVLVSLQGLVLNSK
Sbjct: 962  PSAYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSSILQVLVSLQGLVLNSK 1021

Query: 1035 PYFNEAGYDKQIGTAEGEKNSLSYNENTFLLNCKTIMYLMRKPPKDFEELVKEHFRRRGY 1094
            PYFNEAGYDKQ+GTAEGEKNSLSYNENTFLLNCKTIMYLMRKPPKDFEEL+KEHFR+RGY
Sbjct: 1022 PYFNEAGYDKQVGTAEGEKNSLSYNENTFLLNCKTIMYLMRKPPKDFEELIKEHFRKRGY 1081

Query: 1095 YILKACDAYMKGHLIGSLTEDASIREESDPNSTSVGFKLMLAKIVPKLFSSLNEVGADCR 1154
            +ILKACDAYMKGHLIGSLTEDASIREESDPNSTSVGFKLMLAKIVPKLFSSLNEVGADC+
Sbjct: 1082 FILKACDAYMKGHLIGSLTEDASIREESDPNSTSVGFKLMLAKIVPKLFSSLNEVGADCQ 1141

Query: 1155 DFKHFQQL 1162
            DF HFQQL
Sbjct: 1142 DFMHFQQL 1141

BLAST of Carg05185-RA vs. TrEMBL
Match: tr|A0A2I4EWP2|A0A2I4EWP2_9ROSI (probable ubiquitin-conjugating enzyme E2 23 OS=Juglans regia OX=51240 GN=LOC108993378 PE=4 SV=1)

HSP 1 Score: 1423.7 bits (3684), Expect = 0.0e+00
Identity = 792/1150 (68.87%), Postives = 908/1150 (78.96%), Query Frame = 0

Query: 21   VDEESLTDTCDSYMNRDGVSTSGNIHMPTINSD--EARRMNENRSDKHNIPHVYRRDIVK 80
            V+E + +   ++   +D  +TSG +  P+++S+  E  +  +  S+ HNIP++YR+D+VK
Sbjct: 12   VNEPTTSSHDNNSPKQDYSTTSGLVCDPSVDSESKEVSKPMDTASNTHNIPYIYRQDVVK 71

Query: 81   SKGSGMIGIVTEVAGDADSDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 140
               SGM GIVTEVAG+        XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 72   RNSSGMTGIVTEVAGET-------XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 131

Query: 141  XXXXXXXXXXXXXXXQPLPADEVRVLWMDESETTQNVNDLTVIDRGFLHGDFVAAVSNPT 200
            XXXXXXX         PLPAD+VRVLWMDE+E+T N+ND+ V+DRGFLHGDFVAA S+PT
Sbjct: 132  XXXXXXXWKRGGGKSDPLPADQVRVLWMDETESTVNLNDVAVVDRGFLHGDFVAAASDPT 191

Query: 201  GQAGVVVDVNISVDLLVPDGSVVKNISSKDLIRVRDFAVGDYVVLGPWLGRIDDVLDNVT 260
            GQ GVVVDVNISVDLL PDGSV+K++ SKDL RVRDF VGDYVVLGPWLGRIDDVLDNVT
Sbjct: 192  GQVGVVVDVNISVDLLAPDGSVMKDVPSKDLKRVRDFTVGDYVVLGPWLGRIDDVLDNVT 251

Query: 261  VMFDDGSKCKVTKAEPSRLKPVSKNILEDASFPYYPGQRVRASSSTVFKNSKWLSGLWKP 320
            V+FDDGS CKV KAEP RLKP+SKNILED  FPYYPGQRV+ASSS+VFKNS+WLSGLWK 
Sbjct: 252  VLFDDGSVCKVPKAEPLRLKPISKNILEDGHFPYYPGQRVKASSSSVFKNSRWLSGLWKA 311

Query: 321  NRLQGTVTKVTVGSVFIYWIASAGYGPDSSTTPAEEQSPKNLRLLTCFSHVNWQLGDWCL 380
            NRL+GTVTKVTVGSVFIYWIASAGYGPDSST PAEEQSPKNL+LL+CFSH NWQLGDWCL
Sbjct: 312  NRLEGTVTKVTVGSVFIYWIASAGYGPDSSTAPAEEQSPKNLKLLSCFSHANWQLGDWCL 371

Query: 381  LPSSFSSGS---TKDLSQTELSDSVTNTLDYAQTVGACDSEDTVVDELSGRTESTDLDSI 440
            LPS     S      LS+ EL  S+   LD  Q    CDSE+  ++E +  +ES DLD +
Sbjct: 372  LPSLALPSSIPLDSGLSKLELHHSINGELDSTQLGSGCDSEEISLEESNENSESMDLDPV 431

Query: 441  SACNGNYRDPECNILPES---SSSRATKENTHETWPLHRKKIRKVVIRRDKXXXXXXXNF 500
             A + N R+   N    S   SS   +KE  HE+WPLHRKKIRKVV+RRD+       +F
Sbjct: 432  PAVDENDRNFPINETESSSCGSSLSVSKEPVHESWPLHRKKIRKVVVRRDRKTRKKEESF 491

Query: 501  ERALLIINTTTKVDVVWQDGRTELALDSTGLIPI-NPGDHEFVPEQYVVEKATDNDDDVT 560
            ERALLI+NT TKVDV WQDG+T   LDST LIPI +PGDHEFV EQYVVEKA+D+ DDV 
Sbjct: 492  ERALLIVNTVTKVDVAWQDGKTGHWLDSTSLIPIDSPGDHEFVAEQYVVEKASDDGDDVG 551

Query: 561  ESRRVGVVKSVHAKERTACVRWLKPVSRAEDPREFDIEEVVSVYELEGHPDYDYCYGDVV 620
            E RRVG+VKSV+AKERTACVRWLKPV+RAEDPREFD EE+VSVYELEGHPDYDYCYGDVV
Sbjct: 552  EIRRVGIVKSVNAKERTACVRWLKPVTRAEDPREFDKEEIVSVYELEGHPDYDYCYGDVV 611

Query: 621  VRLSPVSDSIEALSSENNTEELNQQSSTNGVMSCPEKVSGSLKIEYPSSSDDCFDFSDLS 680
            VRLSPVS S E  +  ++ ++  QQ+  + V    ++ SG  K++  S    C  FSDLS
Sbjct: 612  VRLSPVSVSAETATGIDSIDQAKQQNGLDEVKQDMKRHSGCKKVDDASEYKACSGFSDLS 671

Query: 681  WVGNITNLKNGDIEVFWANGMVSTVGPQAIYVVGRDDDDESTGAGSEISNGAASWETVXX 740
            WVGNIT L NGDIEV WA+GMVSTVGPQAIYVVGRDDDDES  AGSEIS+ AASWETV  
Sbjct: 672  WVGNITGLSNGDIEVTWADGMVSTVGPQAIYVVGRDDDDESIAAGSEISDDAASWETV-- 731

Query: 741  XXXXMD-VENAKEDIELQDTGVDSEEEESEQHNSGRSLALSVPLAAFRFVTRLATGIFSR 800
                MD +ENA+E+  L D    + E E    NSG++ ALSVPLAA RFVTRLATGIFSR
Sbjct: 732  NDNEMDALENAQEEDGLHDAHYTNSEPEESGENSGKNTALSVPLAALRFVTRLATGIFSR 791

Query: 801  GPRNRESMDLDSHSESDTQSLEIQSLEEIDSGLQSSSLKSNSFTTSDMNSDCGRGEDDDA 860
            G +N +   LDS  ES+ +S  I  +    SG + SS +S+S  ++ ++S     +++ A
Sbjct: 792  GQKNLDPSCLDSKVESELESQRIIQI----SGGKDSSDESSSQISNVIDSFGTDFKEEVA 851

Query: 861  SEPSKMLVSAEASSNLRTAQSDGSACHEDGTCSFKGFDIAKDPLDHYFLGTNGQTINGRK 920
            SE  K+  +AEAS NLRT +S+  AC  D TC FK FDIAKDP+DHYFLG  GQ  NGRK
Sbjct: 852  SEAQKLFETAEASGNLRTEESNAPACSVDDTCGFKHFDIAKDPMDHYFLGAYGQNNNGRK 911

Query: 921  WLKKIQQDWTILQNNLPDGIYVRVYEDRMDLLRTVIVGAYGTPYQDGLFFFDFHLPPEYP 980
            W KK+QQDW+ILQNNLPD IYVRVYEDRMDLLR VIVGAYGTPYQDGLFFFDFHLPPEYP
Sbjct: 912  WYKKVQQDWSILQNNLPDTIYVRVYEDRMDLLRAVIVGAYGTPYQDGLFFFDFHLPPEYP 971

Query: 981  DVPPSAYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSSILQVLVSLQGLVL 1040
            DVPPSAYYHSGGWRINPNLYEEGKVCLSLLNTWTG+GNEVWD KSSSILQVLVSLQGLVL
Sbjct: 972  DVPPSAYYHSGGWRINPNLYEEGKVCLSLLNTWTGKGNEVWDSKSSSILQVLVSLQGLVL 1031

Query: 1041 NSKPYFNEAGYDKQIGTAEGEKNSLSYNENTFLLNCKTIMYLMRKPPKDFEELVKEHFRR 1100
            NSKPYFNEAGYD+QIGT EGEKNSLSYNENTFLLNCKTIMYLMRKPPK    LVK+HFR+
Sbjct: 1032 NSKPYFNEAGYDRQIGTPEGEKNSLSYNENTFLLNCKTIMYLMRKPPKXXXXLVKDHFRK 1091

Query: 1101 RGYYILKACDAYMKGHLIGSLTEDASIREESDPNSTSVGFKLMLAKIVPKLFSSLNEVGA 1160
            RGYYILKACDAYMKG LIGSLTEDAS+ ++S+ NSTSVGFKLMLAKI+P+LF +L+EVGA
Sbjct: 1092 RGYYILKACDAYMKGGLIGSLTEDASVSDKSNANSTSVGFKLMLAKILPRLFVALSEVGA 1148

BLAST of Carg05185-RA vs. TrEMBL
Match: tr|A0A2N9EYL6|A0A2N9EYL6_FAGSY (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS7810 PE=4 SV=1)

HSP 1 Score: 1417.9 bits (3669), Expect = 0.0e+00
Identity = 803/1185 (67.76%), Postives = 916/1185 (77.30%), Query Frame = 0

Query: 14   EMGQQDSV--DEESLTDTCD--SYMNRDGVSTSGNIHMPTINSD--EARRMNENRSDKHN 73
            E  Q DSV   +E  T   D  +  N+ G + SG +  P ++S+  E  + N+  S KH 
Sbjct: 2    ENEQYDSVCKVDEPTTSAHDNNNSSNQGGSTPSGLVCDPNVDSESNEVCKPNDAASYKHT 61

Query: 74   IPHVYRRDIVKSKGSGMIGIVTEVAGDADSDXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 133
            IP++YR+D+VKS   GM GIVTE AGD    XXXXXXXXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 62   IPYIYRQDVVKSNSKGMTGIVTEAAGDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 121

Query: 134  XXXXXXXXXXXXXXXXXXXXXXXXXXQPLPADEVRVLWMDESETTQNVNDLTVIDRGFLH 193
            XXXXXXXXXXXX               PL AD+VRVLWMDE+E+T+N+ D+TV+DRGFLH
Sbjct: 122  XXXXXXXXXXXXRGGGKNG--------PLLADQVRVLWMDETESTENIKDVTVVDRGFLH 181

Query: 194  GDFVAAVSNPTGQAGVVVDVNISVDLLVPDGSVVKNISSKDLIRVRDFAVGDYVVLGPWL 253
            GDFVAA S+PTGQ GVVVDVNI VDLL PDGS +K++SSKDL R+RDF VGDYVVLGPWL
Sbjct: 182  GDFVAAASDPTGQVGVVVDVNIFVDLLAPDGSTIKDVSSKDLKRIRDFTVGDYVVLGPWL 241

Query: 254  GRIDDVLDNVTVMFDDGSKCKVTKAEPSRLKPVSKNILEDASFPYYPGQRVRASSSTVFK 313
            GRIDDVLDNVTV+FDDGS CKV+K EP RLKPVSKNILED  FPYYPGQRVRASSS++FK
Sbjct: 242  GRIDDVLDNVTVLFDDGSVCKVSKVEPLRLKPVSKNILEDGHFPYYPGQRVRASSSSLFK 301

Query: 314  NSKWLSGLWKPNRLQGTVTKVTVGSVFIYWIASAGYGPDSSTTPAEEQSPKNLRLLTCFS 373
            NS+WLSGLWK  RL+GTVTKVTVGSVFIYWIASAGYGPDSST PAEEQSPKNL+LL+CF+
Sbjct: 302  NSRWLSGLWKATRLEGTVTKVTVGSVFIYWIASAGYGPDSSTAPAEEQSPKNLKLLSCFA 361

Query: 374  HVNWQLGDWCLLPS---SFSSGSTKDLSQTELSDSVTNTLDYAQTVGACDSEDTVVDELS 433
            H NWQLGDWCL PS   S S+   K LS+ EL DS     D  Q    CDSE+  ++E +
Sbjct: 362  HANWQLGDWCLFPSFALSSSNPLDKGLSKLELHDSANGEFDSTQMGSGCDSEEVSLEESN 421

Query: 434  GRTESTDLDSISACNGNYRDPECNILPESSSS----RATKENTHETWPLHRKKIRKVVIR 493
            G TES DLD ++A +GN R+ E N  PESSS       +KE  HE+WPLHRKKIRKVV+R
Sbjct: 422  GNTESMDLDPVAAVDGNDRNGE-NNEPESSSCGSSLSVSKEPVHESWPLHRKKIRKVVVR 481

Query: 494  RDKXXXXXXXNFERALLIINTTTKVDVVWQDGRTELALDSTGLIPI-NPGDHEFVPEQYV 553
            RDK       ++ERALLI+NT TKVDV WQDG T   LDST LIPI +PGDHEFV EQYV
Sbjct: 482  RDKKARRKEESYERALLIVNTRTKVDVAWQDGTTGCGLDSTSLIPIDSPGDHEFVAEQYV 541

Query: 554  VEKATDNDDDVTESRRVGVVKSVHAKERTACVRWLKPVSRAEDPREFDIEEVVSVYELEG 613
            VEKA+D+DDDV E+RRVGVVKSV+AKERTACVRWLKPV+RAEDPREF+ EEVVSVYELEG
Sbjct: 542  VEKASDDDDDVGEARRVGVVKSVNAKERTACVRWLKPVARAEDPREFEKEEVVSVYELEG 601

Query: 614  HPDYDYCYGDVVVRLSPVSDSIEALSSENNTEELNQQSSTNGVMSCPEKVSGSLKIEYPS 673
            HPDYDYCYGDVVVRLSPV+   E  S  ++ EE  QQ+  N V    +K SGS K+E  S
Sbjct: 602  HPDYDYCYGDVVVRLSPVNVPAETPSGLDSAEEPKQQNGPNEVKRDMKKRSGSKKVEDAS 661

Query: 674  SSDDCFDFSDLSWVGNITNLKNGDIEVFWANGMVSTVGPQAIYVVGRDDDDESTGAGSEI 733
             +  C +F+DLSWVGNIT LKNGDIEV WA+GMVSTVGPQAIYVVGRDDDDES  AGSE+
Sbjct: 662  GNKACSNFTDLSWVGNITGLKNGDIEVTWADGMVSTVGPQAIYVVGRDDDDESLAAGSEV 721

Query: 734  SNGAASWETVXXXXXXMD-VENAKE----------------DIE-----LQDTGVDSEEE 793
            S+ AASWETV      MD ++NA+E                D+E       D  ++S+ E
Sbjct: 722  SDDAASWETV--NDDEMDTLDNAQEVWALLFKDIYIGMHKRDLEDGQHNATDADINSDPE 781

Query: 794  ESEQHNSGRSLALSVPLAAFRFVTRLATGIFSRGPRNRESMDLDSHSESDTQSLE-IQSL 853
            ES          LSVPLAA RFVTRLATGIFSRG +N +   LDS  E++  SL+ I+  
Sbjct: 782  ES-XXXXXXXXXLSVPLAALRFVTRLATGIFSRGQKNLDPSCLDSKVENELGSLQTIKIT 841

Query: 854  EEIDSGLQSSSLKSNSFTTSDMNSDCG-RGEDDDASEPSKMLVSAEASSNLRTAQSDGSA 913
            E  DS  +SSS KSN      +   CG   +++   E  ++  +A+A  NL   +S+  A
Sbjct: 842  EGKDSNDESSSQKSN------IIDSCGIEIKEEVTPEAKELFEAAQALCNLSAEESNPPA 901

Query: 914  CHEDGTCSFKGFDIAKDPLDHYFLGTNGQTINGRKWLKKIQQDWTILQNNLPDGIYVRVY 973
            C +D TCSFK FD AKDPLDHYFLG  GQ  NGR+W KK+QQDW+ILQNNLP  IYVRVY
Sbjct: 902  CSDDDTCSFKHFDTAKDPLDHYFLGAYGQNNNGRRWFKKVQQDWSILQNNLPYTIYVRVY 961

Query: 974  EDRMDLLRTVIVGAYGTPYQDGLFFFDFHLPPEYPDVPPSAYYHSGGWRINPNLYEEGKV 1033
            EDRMDLLR VIVGAYGTPYQDGLF FDFHLPPEYPDVPPSAYYHSGGWRINPNLYEEGKV
Sbjct: 962  EDRMDLLRAVIVGAYGTPYQDGLFCFDFHLPPEYPDVPPSAYYHSGGWRINPNLYEEGKV 1021

Query: 1034 CLSLLNTWTGRGNEVWDPKSSSILQVLVSLQGLVLNSKPYFNEAGYDKQIGTAEGEKNSL 1093
            CLSLLNTWTG+GNEVWDPKSSSILQVLVSLQGLVLNS+PYFNEAGYDKQ+GTAEGEKNSL
Sbjct: 1022 CLSLLNTWTGKGNEVWDPKSSSILQVLVSLQGLVLNSRPYFNEAGYDKQVGTAEGEKNSL 1081

Query: 1094 SYNENTFLLNCKTIMYLMRKPPKDFEELVKEHFRRRGYYILKACDAYMKGHLIGSLTEDA 1153
            SYNENTFLLNCKT+MYL+RKPPKDFEELVK+HFR+RGYYILKACDAYMKG+LIGSLTEDA
Sbjct: 1082 SYNENTFLLNCKTMMYLIRKPPKDFEELVKDHFRKRGYYILKACDAYMKGYLIGSLTEDA 1141

Query: 1154 SIREESDPNSTSVGFKLMLAKIVPKLFSSLNEVGADCRDFKHFQQ 1161
            S+ ++S+ NSTSVGFKLMLAKIVPKLF +L+EVGADC DF+H QQ
Sbjct: 1142 SVSDKSNANSTSVGFKLMLAKIVPKLFMALSEVGADCHDFRHLQQ 1168