Carg04642-RA (mRNA) Silver-seed gourd

NameCarg04642-RA
TypemRNA
OrganismCucurbita argyrosperma (Silver-seed gourd)
Description1-aminocyclopropane-1-carboxylate oxidase 1
LocationCucurbita_argyrosperma_scaffold_028 : 190019 .. 191588 (+)
Sequence length1236
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TCCAAATTTTTTAAATCCAAAACAATTTCCCCTATAAATACCATCCATTTTCTCTTCATCCACCATAACCAAAATCCCAACAAAGCTCATTCAAGCTTTTTGTATCAGCAAAAAAATGGCCATTTTTCCTGTCATCAACTTGGAGAATATCAATGGAGATGGTAGAGCTAAGATCTTGGAGCAAATTGAAGATGCCTGCCAAAATTGGGGATTCTTTGAGGTGATTCATAGATTACGATTTTTTTTTTTTTTTTTTTTTTACGATGATTTGAACGTGTGACCGTTTAGAGAAAGTAGAGATGTTTTGACCGAACAAGATCTTGGTTTGGTTTGTTGTTCTTTGTTTTGGTCGTGTTTGTAATGGGTTGGAGTTGGTTTTTGCAGCTGGTGAACCATGGGATCCCACATGAGTTCTTGGACACTGTGGAGAAGATGACAAAGGATCATTACAAGAAATGTATGGAACAGAGGTTTAAGGAGGCTGTGGCTAGCAAGGGGTTGGAGGCTGCTCAGGTTGAAGTTAAGGATATGGACTGGGAAAGCACGTTCTTCTTGCGCCATCTCCCCGAGTCGAACATCGCTGAGATGTCGGAACTCGACGAGGAGTACAAGTATGATGATATTGTTTAGTTTGAGCATAAAATTTGTATGGTTATGTTATATACTCTTTCTATAACGTTGCATGTAAATGATTTATAGGGAAATTATGAAGGAATTTGCTAAGAAGATTGAGAATCTTTCAGAGGAGTTATTGGATCTTCTATGTGAGAATCTTGGGTTGGAAAAGGGGTATTTGAAGAAGGTTTTCCATGGATCAAAAGGTCCTACATTTGGGACAAAGGTGAGTAACTACCCGCCGTGTCCAAAGCCGGATTTGATCAAGGGTCTCCGAGCTCATACCGACGCTGGTGGCATCATCCTCCTCTTCCAGGATAACAAGGTCAGCGGCCTGCAACTCCTCAAAGACGGTAATTGGATCGATGTGCCACCGATGCGCCACGCCATCGTTGTAAACCTTGGAGACCAACTTGAGGTAGGTGGTAGGTCACAACAAAATGGTATAAAGTGAAGAAGGAAGGAAGGAAGGAAAGATTTTAATGTTTCTCAATTTGCAGGTGATCACAAATGGAAGATACAAAAGTGTGATGCATAGAGTGATGACTCAGACAAGCGGGGGTGGTCGAATGTCGTTAGCTTCGTTTTACAATCCGGGGAGCGACGCCGTGATCTTCCCGGCGCCGTCGCTGATAGAGGGTGAGGGTGAGGAGAAGAAGGAAGTGTATCCCAAGTTTGTGTTTGAAGATTACATGAAGCTGTATTTGGGAGTGAAGTTTGAGGCGAAGGAGCCAAGATTTGAAGCCATGAAAGGCAATGCTAATTTGGGTCCAATGGCAACAGCATAGAAGAAGAAAAGGAAAGAAAGAAAGACCCACTTTTTTAACAATAAATATTTAAAGTGGGTTAAGTGAGTCACTCAATCTCTAGTCTATTGTTATTATTTTAATCAAAAGTTAGAAAACAATATCGTTTGGTCCCTATTCTCTTTATAATTAAATTCTCTTTCCGTTTG

mRNA sequence

TCCAAATTTTTTAAATCCAAAACAATTTCCCCTATAAATACCATCCATTTTCTCTTCATCCACCATAACCAAAATCCCAACAAAGCTCATTCAAGCTTTTTGTATCAGCAAAAAAATGGCCATTTTTCCTGTCATCAACTTGGAGAATATCAATGGAGATGGTAGAGCTAAGATCTTGGAGCAAATTGAAGATGCCTGCCAAAATTGGGGATTCTTTGAGCTGGTGAACCATGGGATCCCACATGAGTTCTTGGACACTGTGGAGAAGATGACAAAGGATCATTACAAGAAATGTATGGAACAGAGGTTTAAGGAGGCTGTGGCTAGCAAGGGGTTGGAGGCTGCTCAGGTTGAAGTTAAGGATATGGACTGGGAAAGCACGTTCTTCTTGCGCCATCTCCCCGAGTCGAACATCGCTGAGATGTCGGAACTCGACGAGGAGTACAAGGAAATTATGAAGGAATTTGCTAAGAAGATTGAGAATCTTTCAGAGGAGTTATTGGATCTTCTATGTGAGAATCTTGGGTTGGAAAAGGGGTATTTGAAGAAGGTTTTCCATGGATCAAAAGGTCCTACATTTGGGACAAAGGTGAGTAACTACCCGCCGTGTCCAAAGCCGGATTTGATCAAGGGTCTCCGAGCTCATACCGACGCTGGTGGCATCATCCTCCTCTTCCAGGATAACAAGGTCAGCGGCCTGCAACTCCTCAAAGACGGTAATTGGATCGATGTGCCACCGATGCGCCACGCCATCGTTGTAAACCTTGGAGACCAACTTGAGGTGATCACAAATGGAAGATACAAAAGTGTGATGCATAGAGTGATGACTCAGACAAGCGGGGGTGGTCGAATGTCGTTAGCTTCGTTTTACAATCCGGGGAGCGACGCCGTGATCTTCCCGGCGCCGTCGCTGATAGAGGGTGAGGGTGAGGAGAAGAAGGAAGTGTATCCCAAGTTTGTGTTTGAAGATTACATGAAGCTGTATTTGGGAGTGAAGTTTGAGGCGAAGGAGCCAAGATTTGAAGCCATGAAAGGCAATGCTAATTTGGGTCCAATGGCAACAGCATAGAAGAAGAAAAGGAAAGAAAGAAAGACCCACTTTTTTAACAATAAATATTTAAAGTGGGTTAAGTGAGTCACTCAATCTCTAGTCTATTGTTATTATTTTAATCAAAAGTTAGAAAACAATATCGTTTGGTCCCTATTCTCTTTATAATTAAATTCTCTTTCCGTTTG

Coding sequence (CDS)

ATGGCCATTTTTCCTGTCATCAACTTGGAGAATATCAATGGAGATGGTAGAGCTAAGATCTTGGAGCAAATTGAAGATGCCTGCCAAAATTGGGGATTCTTTGAGCTGGTGAACCATGGGATCCCACATGAGTTCTTGGACACTGTGGAGAAGATGACAAAGGATCATTACAAGAAATGTATGGAACAGAGGTTTAAGGAGGCTGTGGCTAGCAAGGGGTTGGAGGCTGCTCAGGTTGAAGTTAAGGATATGGACTGGGAAAGCACGTTCTTCTTGCGCCATCTCCCCGAGTCGAACATCGCTGAGATGTCGGAACTCGACGAGGAGTACAAGGAAATTATGAAGGAATTTGCTAAGAAGATTGAGAATCTTTCAGAGGAGTTATTGGATCTTCTATGTGAGAATCTTGGGTTGGAAAAGGGGTATTTGAAGAAGGTTTTCCATGGATCAAAAGGTCCTACATTTGGGACAAAGGTGAGTAACTACCCGCCGTGTCCAAAGCCGGATTTGATCAAGGGTCTCCGAGCTCATACCGACGCTGGTGGCATCATCCTCCTCTTCCAGGATAACAAGGTCAGCGGCCTGCAACTCCTCAAAGACGGTAATTGGATCGATGTGCCACCGATGCGCCACGCCATCGTTGTAAACCTTGGAGACCAACTTGAGGTGATCACAAATGGAAGATACAAAAGTGTGATGCATAGAGTGATGACTCAGACAAGCGGGGGTGGTCGAATGTCGTTAGCTTCGTTTTACAATCCGGGGAGCGACGCCGTGATCTTCCCGGCGCCGTCGCTGATAGAGGGTGAGGGTGAGGAGAAGAAGGAAGTGTATCCCAAGTTTGTGTTTGAAGATTACATGAAGCTGTATTTGGGAGTGAAGTTTGAGGCGAAGGAGCCAAGATTTGAAGCCATGAAAGGCAATGCTAATTTGGGTCCAATGGCAACAGCATAG

Protein sequence

MAIFPVINLENINGDGRAKILEQIEDACQNWGFFELVNHGIPHEFLDTVEKMTKDHYKKCMEQRFKEAVASKGLEAAQVEVKDMDWESTFFLRHLPESNIAEMSELDEEYKEIMKEFAKKIENLSEELLDLLCENLGLEKGYLKKVFHGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDAGGIILLFQDNKVSGLQLLKDGNWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVMTQTSGGGRMSLASFYNPGSDAVIFPAPSLIEGEGEEKKEVYPKFVFEDYMKLYLGVKFEAKEPRFEAMKGNANLGPMATA
BLAST of Carg04642-RA vs. NCBI nr
Match: XP_022930840.1 (1-aminocyclopropane-1-carboxylate oxidase 1 [Cucurbita moschata])

HSP 1 Score: 639.4 bits (1648), Expect = 6.4e-180
Identity = 315/319 (98.75%), Postives = 315/319 (98.75%), Query Frame = 0

Query: 1   MAIFPVINLENINGDGRAKILEQIEDACQNWGFFELVNHGIPHEFLDTVEKMTKDHYKKC 60
           MAIFPVINLENINGDGRAKILEQIEDACQNWGFFELVNHGIPHEFLDTVEKMTKDHYKKC
Sbjct: 1   MAIFPVINLENINGDGRAKILEQIEDACQNWGFFELVNHGIPHEFLDTVEKMTKDHYKKC 60

Query: 61  MEQRFKEAVASKGLEAAQVEVKDMDWESTFFLRHLPESNIAEMSELDEEYKEIMKEFAKK 120
           MEQRFKEAVASKGLEAAQVEVKDMDWESTFFLRHLPESNIAEMSELDEEYKEIMKEFAKK
Sbjct: 61  MEQRFKEAVASKGLEAAQVEVKDMDWESTFFLRHLPESNIAEMSELDEEYKEIMKEFAKK 120

Query: 121 IENLSEELLDLLCENLGLEKGYLKKVFHGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDA 180
           IENLSEELLDLLCENLGLEKGYLKKVFHGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDA
Sbjct: 121 IENLSEELLDLLCENLGLEKGYLKKVFHGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDA 180

Query: 181 GGIILLFQDNKVSGLQLLKDGNWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVMTQT 240
           GGIILLFQDNKVSGLQLLKDGNWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVMTQT
Sbjct: 181 GGIILLFQDNKVSGLQLLKDGNWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVMTQT 240

Query: 241 SGGGRMSLASFYNPGSDAVIFPAPSLI--EGEGEEKKEVYPKFVFEDYMKLYLGVKFEAK 300
           SGGGRMSLASFYNPGSDAVIFPA SLI  EGEGEEKKEVYPKFVFEDYMKLYLGVKFEAK
Sbjct: 241 SGGGRMSLASFYNPGSDAVIFPAASLIEKEGEGEEKKEVYPKFVFEDYMKLYLGVKFEAK 300

Query: 301 EPRFEAMKGNANLGPMATA 318
           EPRFEAMK NANLGPMATA
Sbjct: 301 EPRFEAMKANANLGPMATA 319

BLAST of Carg04642-RA vs. NCBI nr
Match: XP_022988653.1 (1-aminocyclopropane-1-carboxylate oxidase 1 [Cucurbita maxima])

HSP 1 Score: 636.3 bits (1640), Expect = 5.4e-179
Identity = 312/319 (97.81%), Postives = 315/319 (98.75%), Query Frame = 0

Query: 1   MAIFPVINLENINGDGRAKILEQIEDACQNWGFFELVNHGIPHEFLDTVEKMTKDHYKKC 60
           MAIFPVINLENINGDGRAKILEQIEDACQNWGFFELVNHGIPHEFLD+VEKMTKDHYKKC
Sbjct: 1   MAIFPVINLENINGDGRAKILEQIEDACQNWGFFELVNHGIPHEFLDSVEKMTKDHYKKC 60

Query: 61  MEQRFKEAVASKGLEAAQVEVKDMDWESTFFLRHLPESNIAEMSELDEEYKEIMKEFAKK 120
           MEQRFKEAVASKGLEAAQVEVKDMDWESTFFLRHLPESNIAEMSELDEEYKEIMKEFAKK
Sbjct: 61  MEQRFKEAVASKGLEAAQVEVKDMDWESTFFLRHLPESNIAEMSELDEEYKEIMKEFAKK 120

Query: 121 IENLSEELLDLLCENLGLEKGYLKKVFHGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDA 180
           IENLSEELLDLLCENLGLEKGYLKKVFHGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDA
Sbjct: 121 IENLSEELLDLLCENLGLEKGYLKKVFHGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDA 180

Query: 181 GGIILLFQDNKVSGLQLLKDGNWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVMTQT 240
           GGIILLFQD+KVSGLQLLKDGNWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVMTQT
Sbjct: 181 GGIILLFQDDKVSGLQLLKDGNWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVMTQT 240

Query: 241 SGGGRMSLASFYNPGSDAVIFPAPSLI--EGEGEEKKEVYPKFVFEDYMKLYLGVKFEAK 300
           SGGGRMSLASFYNPGSDAVI+PAPSLI  EGEGEEKKE YPKFVFEDYMKLYLGVKFEAK
Sbjct: 241 SGGGRMSLASFYNPGSDAVIYPAPSLIEKEGEGEEKKEAYPKFVFEDYMKLYLGVKFEAK 300

Query: 301 EPRFEAMKGNANLGPMATA 318
           EPRFEAMK NANLGPMATA
Sbjct: 301 EPRFEAMKANANLGPMATA 319

BLAST of Carg04642-RA vs. NCBI nr
Match: XP_023529224.1 (1-aminocyclopropane-1-carboxylate oxidase 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 634.4 bits (1635), Expect = 2.0e-178
Identity = 312/319 (97.81%), Postives = 314/319 (98.43%), Query Frame = 0

Query: 1   MAIFPVINLENINGDGRAKILEQIEDACQNWGFFELVNHGIPHEFLDTVEKMTKDHYKKC 60
           MAIFPVINLENINGDGRAKILEQ+EDACQNWGFFELVNHGI HEFLDTVEKMTKDHYKKC
Sbjct: 1   MAIFPVINLENINGDGRAKILEQVEDACQNWGFFELVNHGISHEFLDTVEKMTKDHYKKC 60

Query: 61  MEQRFKEAVASKGLEAAQVEVKDMDWESTFFLRHLPESNIAEMSELDEEYKEIMKEFAKK 120
           MEQRFKEAVASKGLEAAQ EVKDMDWESTFFLRHLPESNIAEMSELDEEYKEIMKEFAKK
Sbjct: 61  MEQRFKEAVASKGLEAAQDEVKDMDWESTFFLRHLPESNIAEMSELDEEYKEIMKEFAKK 120

Query: 121 IENLSEELLDLLCENLGLEKGYLKKVFHGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDA 180
           IENLSEELLDLLCENLGLEKGYLKKVFHGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDA
Sbjct: 121 IENLSEELLDLLCENLGLEKGYLKKVFHGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDA 180

Query: 181 GGIILLFQDNKVSGLQLLKDGNWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVMTQT 240
           GGIILLFQD+KVSGLQLLKDGNWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVMTQT
Sbjct: 181 GGIILLFQDDKVSGLQLLKDGNWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVMTQT 240

Query: 241 SGGGRMSLASFYNPGSDAVIFPAPSLI--EGEGEEKKEVYPKFVFEDYMKLYLGVKFEAK 300
           SGGGRMSLASFYNPGSDAVIFPAPSLI  EGEGEEKKEVYPKFVFEDYMKLYLGVKFEAK
Sbjct: 241 SGGGRMSLASFYNPGSDAVIFPAPSLIEKEGEGEEKKEVYPKFVFEDYMKLYLGVKFEAK 300

Query: 301 EPRFEAMKGNANLGPMATA 318
           EPRFEAMK NANLGPMATA
Sbjct: 301 EPRFEAMKANANLGPMATA 319

BLAST of Carg04642-RA vs. NCBI nr
Match: ADX66712.1 (ACO1 protein [Cucurbita pepo])

HSP 1 Score: 630.9 bits (1626), Expect = 2.3e-177
Identity = 310/319 (97.18%), Postives = 314/319 (98.43%), Query Frame = 0

Query: 1   MAIFPVINLENINGDGRAKILEQIEDACQNWGFFELVNHGIPHEFLDTVEKMTKDHYKKC 60
           MA+FPVINLENINGDGRAKILEQIEDACQNWGFFELVNHGI HEFLDTVEKMTKDHYKKC
Sbjct: 1   MAVFPVINLENINGDGRAKILEQIEDACQNWGFFELVNHGISHEFLDTVEKMTKDHYKKC 60

Query: 61  MEQRFKEAVASKGLEAAQVEVKDMDWESTFFLRHLPESNIAEMSELDEEYKEIMKEFAKK 120
           MEQRFKEAVASKGLEAAQ EVKDMDWESTFFLRHLPESNIAEMSELDE+YKEIMKEFAKK
Sbjct: 61  MEQRFKEAVASKGLEAAQDEVKDMDWESTFFLRHLPESNIAEMSELDEKYKEIMKEFAKK 120

Query: 121 IENLSEELLDLLCENLGLEKGYLKKVFHGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDA 180
           IENLSEELLDLLCE+LGLEKGYLKKVFHGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDA
Sbjct: 121 IENLSEELLDLLCEDLGLEKGYLKKVFHGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDA 180

Query: 181 GGIILLFQDNKVSGLQLLKDGNWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVMTQT 240
           GGIILLFQD+KVSGLQLLKDGNWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVMTQT
Sbjct: 181 GGIILLFQDDKVSGLQLLKDGNWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVMTQT 240

Query: 241 SGGGRMSLASFYNPGSDAVIFPAPSLI--EGEGEEKKEVYPKFVFEDYMKLYLGVKFEAK 300
           SGGGRMSLASFYNPGSDAVIFPAPSLI  EGEGEEKKEVYPKFVFEDYMKLYLGVKFEAK
Sbjct: 241 SGGGRMSLASFYNPGSDAVIFPAPSLIEKEGEGEEKKEVYPKFVFEDYMKLYLGVKFEAK 300

Query: 301 EPRFEAMKGNANLGPMATA 318
           EPRFEAMK NANLGPMATA
Sbjct: 301 EPRFEAMKANANLGPMATA 319

BLAST of Carg04642-RA vs. NCBI nr
Match: NP_001284392.1 (1-aminocyclopropane-1-carboxylate oxidase 1 [Cucumis melo] >Q04644.1 RecName: Full=1-aminocyclopropane-1-carboxylate oxidase 1; Short=ACC oxidase 1; AltName: Full=Ethylene-forming enzyme; Short=EFE; AltName: Full=PMEL1 >CAA49553.1 enzyme-forming ethylene [Cucumis melo] >BAA06526.1 1-aminocyclopropane-1-carboxylate oxidase [Cucumis melo] >CAA64797.1 ACC oxidase [Cucumis melo] >ACY08855.1 ACC oxidase [Cucumis melo])

HSP 1 Score: 599.7 bits (1545), Expect = 5.6e-168
Identity = 287/318 (90.25%), Postives = 306/318 (96.23%), Query Frame = 0

Query: 1   MAIFPVINLENINGDGRAKILEQIEDACQNWGFFELVNHGIPHEFLDTVEKMTKDHYKKC 60
           MA+FP+INLENIN DGRAKILEQIEDACQNWGFFELVNHGIPHEFLD VEKMT+DHYKKC
Sbjct: 1   MAVFPIINLENINDDGRAKILEQIEDACQNWGFFELVNHGIPHEFLDMVEKMTRDHYKKC 60

Query: 61  MEQRFKEAVASKGLEAAQVEVKDMDWESTFFLRHLPESNIAEMSELDEEYKEIMKEFAKK 120
           ME+RFKE V SKGLEAAQ EV DMDWESTFFLRHLPESNI++MS+LDEEYK+IMKEFAKK
Sbjct: 61  MEERFKETVLSKGLEAAQAEVNDMDWESTFFLRHLPESNISQMSDLDEEYKKIMKEFAKK 120

Query: 121 IENLSEELLDLLCENLGLEKGYLKKVFHGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDA 180
           +ENL+EELLDLLCENLGLEKGYLKK F+GSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDA
Sbjct: 121 LENLAEELLDLLCENLGLEKGYLKKAFYGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDA 180

Query: 181 GGIILLFQDNKVSGLQLLKDGNWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVMTQT 240
           GGIILLFQD+KVSGLQLLKDGNWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRV+TQT
Sbjct: 181 GGIILLFQDDKVSGLQLLKDGNWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVLTQT 240

Query: 241 SGGGRMSLASFYNPGSDAVIFPAPSLIE-GEGEEKKEVYPKFVFEDYMKLYLGVKFEAKE 300
           SG GRMS+ASFYNPGSDAVI+PAP+L+E  + EEKKEVYPKFVFEDYMKLYLGVKF+AKE
Sbjct: 241 SGTGRMSIASFYNPGSDAVIYPAPALVEKDQDEEKKEVYPKFVFEDYMKLYLGVKFQAKE 300

Query: 301 PRFEAMKGNANLGPMATA 318
           PRFEAMK NANLGPMATA
Sbjct: 301 PRFEAMKANANLGPMATA 318

BLAST of Carg04642-RA vs. TAIR10
Match: AT1G05010.1 (ethylene-forming enzyme)

HSP 1 Score: 500.0 bits (1286), Expect = 1.1e-141
Identity = 237/323 (73.37%), Postives = 281/323 (87.00%), Query Frame = 0

Query: 1   MAIFPVINLENINGDGRAKILEQIEDACQNWGFFELVNHGIPHEFLDTVEKMTKDHYKKC 60
           M  FP+INLE +NG+ RA  +E+I+DAC+NWGFFE VNHGI  E LD VEKMTK+HYKKC
Sbjct: 1   MESFPIINLEKLNGEERAITMEKIKDACENWGFFECVNHGISLELLDKVEKMTKEHYKKC 60

Query: 61  MEQRFKEAVASKGLEAAQVEVKDMDWESTFFLRHLPESNIAEMSELDEEYKEIMKEFAKK 120
           ME+RFKE++ ++GL++ + EV D+DWESTF+L+HLP SNI+++ +LD++Y+ +MK+FA K
Sbjct: 61  MEERFKESIKNRGLDSLRSEVNDVDWESTFYLKHLPVSNISDVPDLDDDYRTLMKDFAGK 120

Query: 121 IENLSEELLDLLCENLGLEKGYLKKVFHGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDA 180
           IE LSEELLDLLCENLGLEKGYLKKVF+GSK PTFGTKVSNYPPCP PDL+KGLRAHTDA
Sbjct: 121 IEKLSEELLDLLCENLGLEKGYLKKVFYGSKRPTFGTKVSNYPPCPNPDLVKGLRAHTDA 180

Query: 181 GGIILLFQDNKVSGLQLLKDGNWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVMTQT 240
           GGIILLFQD+KVSGLQLLKDG W+DVPP++H+IVVNLGDQLEVITNG+YKSV HRV++QT
Sbjct: 181 GGIILLFQDDKVSGLQLLKDGEWVDVPPVKHSIVVNLGDQLEVITNGKYKSVEHRVLSQT 240

Query: 241 SGGGRMSLASFYNPGSDAVIFPAPSLIEGEGE-EKKEVYPKFVFEDYMKLYLGVKFEAKE 300
            G GRMS+ASFYNPGSD+VIFPAP LI  E E EKKE YP+FVFEDYMKLY  VKF+AKE
Sbjct: 241 DGEGRMSIASFYNPGSDSVIFPAPELIGKEAEKEKKENYPRFVFEDYMKLYSAVKFQAKE 300

Query: 301 PRFEAMKGNA-----NLGPMATA 318
           PRFEAMK        N+GP+ATA
Sbjct: 301 PRFEAMKAMETTVANNVGPLATA 323

BLAST of Carg04642-RA vs. TAIR10
Match: AT1G62380.1 (ACC oxidase 2)

HSP 1 Score: 463.4 bits (1191), Expect = 1.1e-130
Identity = 210/303 (69.31%), Postives = 261/303 (86.14%), Query Frame = 0

Query: 4   FPVINLENINGDGRAKILEQIEDACQNWGFFELVNHGIPHEFLDTVEKMTKDHYKKCMEQ 63
           FPV++L  +NG+ R + +  I +AC+NWGFFE+VNHG+PH+ +D +EKMTKDHYK C EQ
Sbjct: 7   FPVVDLSKLNGEERDQTMALINEACENWGFFEIVNHGLPHDLMDKIEKMTKDHYKTCQEQ 66

Query: 64  RFKEAVASKGLEAAQVEVKDMDWESTFFLRHLPESNIAEMSELDEEYKEIMKEFAKKIEN 123
           +F + + SKGL+  + EV+D+DWESTF++RHLP+SN+ ++S++ +EY+  MK+F K++EN
Sbjct: 67  KFNDMLKSKGLDNLETEVEDVDWESTFYVRHLPQSNLNDISDVSDEYRTAMKDFGKRLEN 126

Query: 124 LSEELLDLLCENLGLEKGYLKKVFHGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDAGGI 183
           L+E+LLDLLCENLGLEKGYLKKVFHG+KGPTFGTKVSNYPPCPKP++IKGLRAHTDAGGI
Sbjct: 127 LAEDLLDLLCENLGLEKGYLKKVFHGTKGPTFGTKVSNYPPCPKPEMIKGLRAHTDAGGI 186

Query: 184 ILLFQDNKVSGLQLLKDGNWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVMTQTSGG 243
           ILLFQD+KVSGLQLLKDG+WIDVPP+ H+IV+NLGDQLEVITNG+YKSV+HRV+TQ   G
Sbjct: 187 ILLFQDDKVSGLQLLKDGDWIDVPPLNHSIVINLGDQLEVITNGKYKSVLHRVVTQQE-G 246

Query: 244 GRMSLASFYNPGSDAVIFPAPSLIEGEGEEKKEVYPKFVFEDYMKLYLGVKFEAKEPRFE 303
            RMS+ASFYNPGSDA I PA SL+E + E     YP FVF+DYMKLY GVKF+ KEPRF 
Sbjct: 247 NRMSVASFYNPGSDAEISPATSLVEKDSE-----YPSFVFDDYMKLYAGVKFQPKEPRFA 303

Query: 304 AMK 307
           AMK
Sbjct: 307 AMK 303

BLAST of Carg04642-RA vs. TAIR10
Match: AT1G12010.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 460.3 bits (1183), Expect = 9.6e-130
Identity = 214/315 (67.94%), Postives = 266/315 (84.44%), Query Frame = 0

Query: 4   FPVINLENINGDGRAKILEQIEDACQNWGFFELVNHGIPHEFLDTVEKMTKDHYKKCMEQ 63
           FPVI+L  +NG+ R + +  I+DACQNWGFFELVNHG+P++ +D +E+MTK+HYKK MEQ
Sbjct: 7   FPVIDLSKLNGEERDQTMALIDDACQNWGFFELVNHGLPYDLMDNIERMTKEHYKKHMEQ 66

Query: 64  RFKEAVASKGLEAAQVEVKDMDWESTFFLRHLPESNIAEMSELDEEYKEIMKEFAKKIEN 123
           +FKE + SKGL+  + EV+D+DWESTF+L HLP+SN+ ++ ++  EY+  MK+F K++E 
Sbjct: 67  KFKEMLRSKGLDTLETEVEDVDWESTFYLHHLPQSNLYDIPDMSNEYRLAMKDFGKRLEI 126

Query: 124 LSEELLDLLCENLGLEKGYLKKVFHGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDAGGI 183
           L+EELLDLLCENLGLEKGYLKKVFHG+ GPTF TK+SNYPPCPKP++IKGLRAHTDAGG+
Sbjct: 127 LAEELLDLLCENLGLEKGYLKKVFHGTTGPTFATKLSNYPPCPKPEMIKGLRAHTDAGGL 186

Query: 184 ILLFQDNKVSGLQLLKDGNWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVMTQTSGG 243
           ILLFQD+KVSGLQLLKDG+W+DVPP++H+IV+NLGDQLEVITNG+YKSVMHRVMTQ   G
Sbjct: 187 ILLFQDDKVSGLQLLKDGDWVDVPPLKHSIVINLGDQLEVITNGKYKSVMHRVMTQKE-G 246

Query: 244 GRMSLASFYNPGSDAVIFPAPSLIEGEGEEKKEVYPKFVFEDYMKLYLGVKFEAKEPRFE 303
            RMS+ASFYNPGSDA I PA SL+     +K   YP FVF+DYMKLY G+KF+AKEPRFE
Sbjct: 247 NRMSIASFYNPGSDAEISPATSLV-----DKDSKYPSFVFDDYMKLYAGLKFQAKEPRFE 306

Query: 304 AMK---GNANLGPMA 316
           AMK     A+L P+A
Sbjct: 307 AMKNAEAAADLNPVA 315

BLAST of Carg04642-RA vs. TAIR10
Match: AT1G77330.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 295.0 bits (754), Expect = 5.3e-80
Identity = 156/316 (49.37%), Postives = 211/316 (66.77%), Query Frame = 0

Query: 1   MAIFPVINLENINGDGRAKILEQIEDACQNWGFFELVNHGIPHEFLDTVEKMTKDHYKKC 60
           MAI PVI+   +NG+ R K L +I  AC+ WGFF+LVNHGIP E L+ V+K++ D YK  
Sbjct: 1   MAI-PVIDFSKLNGEEREKTLSEIARACEEWGFFQLVNHGIPLELLNKVKKLSSDCYKTE 60

Query: 61  MEQRFKEAVASKGL-----EAAQVEVKDMDWESTFFLRHLPESNIAEMSELDEEYKEIMK 120
            E+ FK +   K L     + +  +++++DWE  F L           +E     KE M 
Sbjct: 61  REEAFKTSNPVKLLNELVQKNSGEKLENVDWEDVFTLLD------HNQNEWPSNIKETMG 120

Query: 121 EFAKKIENLSEELLDLLCENLGLEKGYLKKVFH-----GSKGPTFGTKVSNYPPCPKPDL 180
           E+ +++  L+ ++++++ ENLGL KGY+KK F+     G +   FGTKVS+YPPCP P+L
Sbjct: 121 EYREEVRKLASKMMEVMDENLGLPKGYIKKAFNEGMEDGEETAFFGTKVSHYPPCPHPEL 180

Query: 181 IKGLRAHTDAGGIILLFQDNKVSGLQLLKDGNWIDVPPMRHAIVVNLGDQLEVITNGRYK 240
           + GLRAHTDAGG++LLFQD++  GLQ+LKDG WIDV P+ +AIV+N GDQ+EV++NGRYK
Sbjct: 181 VNGLRAHTDAGGVVLLFQDDEYDGLQVLKDGEWIDVQPLPNAIVINTGDQIEVLSNGRYK 240

Query: 241 SVMHRVMTQTSGGGRMSLASFYNPGSDAVIFPAPSLIEGEGEEKKEVYPKFVFEDYMKLY 300
           S  HRV+ +   G R S+ASFYNP   A I PA ++ E EG EKK  YPKFVF DYM +Y
Sbjct: 241 SAWHRVLAREE-GNRRSIASFYNPSYKAAIGPA-AVAEEEGSEKK--YPKFVFGDYMDVY 300

Query: 301 LGVKFEAKEPRFEAMK 307
              KF  KEPRF A+K
Sbjct: 301 ANQKFMPKEPRFLAVK 305

BLAST of Carg04642-RA vs. TAIR10
Match: AT2G19590.1 (ACC oxidase 1)

HSP 1 Score: 280.0 bits (715), Expect = 1.8e-75
Identity = 138/309 (44.66%), Postives = 195/309 (63.11%), Query Frame = 0

Query: 5   PVINLENINGDGRAKILEQIEDACQNWGFFELVNHGIPHEFLDTVEKMTKDHYKKCMEQR 64
           PVI+   ++G+ R+K +  ++ AC  WGFF + NHGI  E ++ V+KM   HY++ ++++
Sbjct: 12  PVIDFAELDGEKRSKTMSLLDHACDKWGFFMVDNHGIDKELMEKVKKMINSHYEEHLKEK 71

Query: 65  FKEAVASKGLEAAQVEVKDMDWESTFFLRHLPESNIAEMSELDEEYKEIMKEFAKKIENL 124
           F ++   K L  ++ +  D DWES+FF+ H P SNI ++  + EE  + M E        
Sbjct: 72  FYQSEMVKAL--SEGKTSDADWESSFFISHKPTSNICQIPNISEELSKTMDEXXXXXXXX 131

Query: 125 SEELLDLLCENLGLEKGYLKKVFHGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDAGGII 184
                     NLGL++  +   F G KGP FGTKV+ YP CP+P+L++GLR HTDAGGII
Sbjct: 132 XXXXXXXXXXNLGLDQEDIMNAFSGPKGPAFGTKVAKYPECPRPELMRGLREHTDAGGII 191

Query: 185 LLFQDNKVSGLQLLKDGNWIDVPPMR-HAIVVNLGDQLEVITNGRYKSVMHRVMTQTSGG 244
           LL QD++V GL+  KDG W+ +PP + + I VN GDQLE+++NGRYKSV+HRVMT    G
Sbjct: 192 LLLQDDQVPGLEFFKDGKWVPIPPSKNNTIFVNTGDQLEILSNGRYKSVVHRVMT-VKHG 251

Query: 245 GRMSLASFYNPGSDAVIFPAPSLIEGEGEEKKEVYPKFVFEDYMKLYLGVKFEAKEPRFE 304
            R+S+A+FYNP  DA+I PAP L+   G         + F+DY+KLY   KF  K PR E
Sbjct: 252 SRLSIATFYNPAGDAIISPAPKLLYPSG---------YRFQDYLKLYSTTKFGDKGPRLE 308

Query: 305 AMK--GNAN 311
            MK  GNA+
Sbjct: 312 TMKKMGNAD 308

BLAST of Carg04642-RA vs. Swiss-Prot
Match: sp|Q04644|ACCO1_CUCME (1-aminocyclopropane-1-carboxylate oxidase 1 OS=Cucumis melo OX=3656 GN=ACO1 PE=2 SV=1)

HSP 1 Score: 599.7 bits (1545), Expect = 1.8e-170
Identity = 287/318 (90.25%), Postives = 306/318 (96.23%), Query Frame = 0

Query: 1   MAIFPVINLENINGDGRAKILEQIEDACQNWGFFELVNHGIPHEFLDTVEKMTKDHYKKC 60
           MA+FP+INLENIN DGRAKILEQIEDACQNWGFFELVNHGIPHEFLD VEKMT+DHYKKC
Sbjct: 1   MAVFPIINLENINDDGRAKILEQIEDACQNWGFFELVNHGIPHEFLDMVEKMTRDHYKKC 60

Query: 61  MEQRFKEAVASKGLEAAQVEVKDMDWESTFFLRHLPESNIAEMSELDEEYKEIMKEFAKK 120
           ME+RFKE V SKGLEAAQ EV DMDWESTFFLRHLPESNI++MS+LDEEYK+IMKEFAKK
Sbjct: 61  MEERFKETVLSKGLEAAQAEVNDMDWESTFFLRHLPESNISQMSDLDEEYKKIMKEFAKK 120

Query: 121 IENLSEELLDLLCENLGLEKGYLKKVFHGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDA 180
           +ENL+EELLDLLCENLGLEKGYLKK F+GSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDA
Sbjct: 121 LENLAEELLDLLCENLGLEKGYLKKAFYGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDA 180

Query: 181 GGIILLFQDNKVSGLQLLKDGNWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVMTQT 240
           GGIILLFQD+KVSGLQLLKDGNWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRV+TQT
Sbjct: 181 GGIILLFQDDKVSGLQLLKDGNWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVLTQT 240

Query: 241 SGGGRMSLASFYNPGSDAVIFPAPSLIE-GEGEEKKEVYPKFVFEDYMKLYLGVKFEAKE 300
           SG GRMS+ASFYNPGSDAVI+PAP+L+E  + EEKKEVYPKFVFEDYMKLYLGVKF+AKE
Sbjct: 241 SGTGRMSIASFYNPGSDAVIYPAPALVEKDQDEEKKEVYPKFVFEDYMKLYLGVKFQAKE 300

Query: 301 PRFEAMKGNANLGPMATA 318
           PRFEAMK NANLGPMATA
Sbjct: 301 PRFEAMKANANLGPMATA 318

BLAST of Carg04642-RA vs. Swiss-Prot
Match: sp|P05116|ACCO1_SOLLC (1-aminocyclopropane-1-carboxylate oxidase 1 OS=Solanum lycopersicum OX=4081 GN=ACO1 PE=2 SV=2)

HSP 1 Score: 519.6 bits (1337), Expect = 2.4e-146
Identity = 247/317 (77.92%), Postives = 281/317 (88.64%), Query Frame = 0

Query: 1   MAIFPVINLENINGDGRAKILEQIEDACQNWGFFELVNHGIPHEFLDTVEKMTKDHYKKC 60
           M  FP+INLE +NGD RA  +E I+DAC+NWGFFELVNHGIPHE +DTVEKMTK HYKKC
Sbjct: 1   MENFPIINLEKLNGDERANTMEMIKDACENWGFFELVNHGIPHEVMDTVEKMTKGHYKKC 60

Query: 61  MEQRFKEAVASKGLEAAQVEVKDMDWESTFFLRHLPESNIAEMSELDEEYKEIMKEFAKK 120
           MEQRFKE VASKGLEA Q EV D+DWESTFFLRHLP SNI+++ +LDEEY+E+M++FAK+
Sbjct: 61  MEQRFKELVASKGLEAVQAEVTDLDWESTFFLRHLPTSNISQVPDLDEEYREVMRDFAKR 120

Query: 121 IENLSEELLDLLCENLGLEKGYLKKVFHGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDA 180
           +E L+EELLDLLCENLGLEKGYLK  F+GSKGP FGTKVSNYPPCPKPDLIKGLRAHTDA
Sbjct: 121 LEKLAEELLDLLCENLGLEKGYLKNAFYGSKGPNFGTKVSNYPPCPKPDLIKGLRAHTDA 180

Query: 181 GGIILLFQDNKVSGLQLLKDGNWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVMTQT 240
           GGIILLFQD+KVSGLQLLKD  WIDVPPMRH+IVVNLGDQLEVITNG+YKSV+HRV+ QT
Sbjct: 181 GGIILLFQDDKVSGLQLLKDEQWIDVPPMRHSIVVNLGDQLEVITNGKYKSVLHRVIAQT 240

Query: 241 SGGGRMSLASFYNPGSDAVIFPAPSLIEGEGEEKKEVYPKFVFEDYMKLYLGVKFEAKEP 300
             G RMSLASFYNPGSDAVI+PA +L+E E EE  +VYPKFVF+DYMKLY G+KF+AKEP
Sbjct: 241 D-GTRMSLASFYNPGSDAVIYPAKTLVEKEAEESTQVYPKFVFDDYMKLYAGLKFQAKEP 300

Query: 301 RFEAMKGNANLGPMATA 318
           RFEAMK   +  P+A+A
Sbjct: 301 RFEAMKAMES-DPIASA 315

BLAST of Carg04642-RA vs. Swiss-Prot
Match: sp|Q43792|ACCO_TOBAC (1-aminocyclopropane-1-carboxylate oxidase OS=Nicotiana tabacum OX=4097 GN=ACO PE=2 SV=1)

HSP 1 Score: 519.2 bits (1336), Expect = 3.1e-146
Identity = 247/320 (77.19%), Postives = 281/320 (87.81%), Query Frame = 0

Query: 1   MAIFPVINLENINGDGRAKILEQIEDACQNWGFFELVNHGIPHEFLDTVEKMTKDHYKKC 60
           M  FP+INLE +NG  RA  +E I+DAC+NWGFFELVNHGIPHE +DTVEKMTK HYKKC
Sbjct: 1   MENFPIINLEKLNGSERADTMEMIKDACENWGFFELVNHGIPHEVMDTVEKMTKGHYKKC 60

Query: 61  MEQRFKEAVASKGLEAAQVEVKDMDWESTFFLRHLPESNIAEMSELDEEYKEIMKEFAKK 120
           MEQRFKE VASKGLEA Q EV D+DWESTFFLRHLP SNI E+ +LD++Y+E+M++FAK+
Sbjct: 61  MEQRFKELVASKGLEAVQAEVTDLDWESTFFLRHLPVSNICEVPDLDDQYREVMRDFAKR 120

Query: 121 IENLSEELLDLLCENLGLEKGYLKKVFHGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDA 180
           +E L+EELLDLLCENLGLEKGYLKK+F+G+KGP FG+KVSNYPPCPKPDLIKGLRAHTDA
Sbjct: 121 LEKLAEELLDLLCENLGLEKGYLKKIFYGTKGPNFGSKVSNYPPCPKPDLIKGLRAHTDA 180

Query: 181 GGIILLFQDNKVSGLQLLKDGNWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVMTQT 240
           GGIILLFQD+KV+GLQLLKDG WIDVPPMR +IVVNLGDQLEVITNG+YKSVMHRV+TQT
Sbjct: 181 GGIILLFQDDKVTGLQLLKDGQWIDVPPMRLSIVVNLGDQLEVITNGKYKSVMHRVITQT 240

Query: 241 SGGGRMSLASFYNPGSDAVIFPAPSLIEGEGEEKKEVYPKFVFEDYMKLYLGVKFEAKEP 300
             G RMSLASFYNPGSDAVIFPAP+L+E E EE K +YPKFVF+DYMKLY G+KF+AKEP
Sbjct: 241 D-GTRMSLASFYNPGSDAVIFPAPTLVEKEAEESKAIYPKFVFDDYMKLYAGLKFQAKEP 300

Query: 301 RFEAM---KGNANLGPMATA 318
           RFEAM          P+ATA
Sbjct: 301 RFEAMIKAMETVKSDPVATA 319

BLAST of Carg04642-RA vs. Swiss-Prot
Match: sp|Q08507|ACCO3_PETHY (1-aminocyclopropane-1-carboxylate oxidase 3 OS=Petunia hybrida OX=4102 GN=ACO3 PE=3 SV=1)

HSP 1 Score: 518.5 bits (1334), Expect = 5.4e-146
Identity = 247/321 (76.95%), Postives = 284/321 (88.47%), Query Frame = 0

Query: 1   MAIFPVINLENINGDGRAKILEQIEDACQNWGFFELVNHGIPHEFLDTVEKMTKDHYKKC 60
           M  FP+INLE +NG  R   +E I+DAC+NWGFFELVNHGIPHE +DTVEK+TK HYKKC
Sbjct: 1   MENFPIINLEKLNGSERDATMEMIKDACENWGFFELVNHGIPHEVMDTVEKLTKGHYKKC 60

Query: 61  MEQRFKEAVASKGLEAAQVEVKDMDWESTFFLRHLPESNIAEMSELDEEYKEIMKEFAKK 120
           MEQRFKE VASKGLEA Q EV D+DWESTFFLRHLP SNI+E+ +LD+EY+E+M++FAK+
Sbjct: 61  MEQRFKELVASKGLEAVQAEVTDLDWESTFFLRHLPVSNISEVPDLDDEYREVMRDFAKR 120

Query: 121 IENLSEELLDLLCENLGLEKGYLKKVFHGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDA 180
           +E L+EELLDLLCENLGLEKGYLKK F+GSKGP FGTKVSNYPPCPKPDLIKGLRAHTDA
Sbjct: 121 LEKLAEELLDLLCENLGLEKGYLKKAFYGSKGPNFGTKVSNYPPCPKPDLIKGLRAHTDA 180

Query: 181 GGIILLFQDNKVSGLQLLKDGNWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVMTQT 240
           GGIILLFQD+KVSGLQLLKDG WIDVPPMRH+IVVNLGDQLEVITNG+YKSV+HRV+ QT
Sbjct: 181 GGIILLFQDDKVSGLQLLKDGQWIDVPPMRHSIVVNLGDQLEVITNGKYKSVLHRVIAQT 240

Query: 241 SGGGRMSLASFYNPGSDAVIFPAPSLIEGEGEEK-KEVYPKFVFEDYMKLYLGVKFEAKE 300
             G RMSLASFYNPGSDAVI+PAP+L+E E +++ K+VYPKFVF+DYMKLY G+KF+AKE
Sbjct: 241 D-GTRMSLASFYNPGSDAVIYPAPTLVEKEADQECKQVYPKFVFDDYMKLYAGLKFQAKE 300

Query: 301 PRFEAMK---GNANLGPMATA 318
           PRFEAMK    +    P+ATA
Sbjct: 301 PRFEAMKAREADVKSDPIATA 320

BLAST of Carg04642-RA vs. Swiss-Prot
Match: sp|P24157|ACCO4_SOLLC (1-aminocyclopropane-1-carboxylate oxidase 4 OS=Solanum lycopersicum OX=4081 GN=ACO4 PE=2 SV=1)

HSP 1 Score: 518.1 bits (1333), Expect = 7.0e-146
Identity = 246/306 (80.39%), Postives = 276/306 (90.20%), Query Frame = 0

Query: 1   MAIFPVINLENINGDGRAKILEQIEDACQNWGFFELVNHGIPHEFLDTVEKMTKDHYKKC 60
           M  FP+INLEN+NGD RAK +E I+DAC+NWGFFELVNHGIPHE +DTVEK+TK HYKKC
Sbjct: 1   MENFPIINLENLNGDERAKTMEMIKDACENWGFFELVNHGIPHEVMDTVEKLTKGHYKKC 60

Query: 61  MEQRFKEAVASKGLEAAQVEVKDMDWESTFFLRHLPESNIAEMSELDEEYKEIMKEFAKK 120
           MEQRFKE VASKGLEA Q EV D+DWESTFFLRHLP SNI+++ +LDEEY+E+M++FAK+
Sbjct: 61  MEQRFKELVASKGLEAVQAEVTDLDWESTFFLRHLPTSNISQVPDLDEEYREVMRDFAKR 120

Query: 121 IENLSEELLDLLCENLGLEKGYLKKVFHGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDA 180
           +E L+EELLDLLCENLGLEKGYLK  F+GSKGP FGTKVSNYPPCPKPDLIKGLRAHTDA
Sbjct: 121 LEKLAEELLDLLCENLGLEKGYLKNAFYGSKGPNFGTKVSNYPPCPKPDLIKGLRAHTDA 180

Query: 181 GGIILLFQDNKVSGLQLLKDGNWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVMTQT 240
           GGIILLFQD+KVSGLQLLKD  WIDVPPMRH+IVVNLGDQLEVITNG+YKSVMHRV+ QT
Sbjct: 181 GGIILLFQDDKVSGLQLLKDEQWIDVPPMRHSIVVNLGDQLEVITNGKYKSVMHRVIAQT 240

Query: 241 SGGGRMSLASFYNPGSDAVIFPAPSLIEGEGEEKKEVYPKFVFEDYMKLYLGVKFEAKEP 300
             G RMSLASFYNPG+DAVI+PAPSLI    EE K+VYPKFVF+DYMKLY G+KF+ KEP
Sbjct: 241 D-GTRMSLASFYNPGNDAVIYPAPSLI----EESKQVYPKFVFDDYMKLYAGLKFQPKEP 300

Query: 301 RFEAMK 307
           RFEAMK
Sbjct: 301 RFEAMK 301

BLAST of Carg04642-RA vs. TrEMBL
Match: tr|H6TBX5|H6TBX5_CUCPE (ACO1 protein OS=Cucurbita pepo OX=3663 PE=2 SV=1)

HSP 1 Score: 630.9 bits (1626), Expect = 1.5e-177
Identity = 310/319 (97.18%), Postives = 314/319 (98.43%), Query Frame = 0

Query: 1   MAIFPVINLENINGDGRAKILEQIEDACQNWGFFELVNHGIPHEFLDTVEKMTKDHYKKC 60
           MA+FPVINLENINGDGRAKILEQIEDACQNWGFFELVNHGI HEFLDTVEKMTKDHYKKC
Sbjct: 1   MAVFPVINLENINGDGRAKILEQIEDACQNWGFFELVNHGISHEFLDTVEKMTKDHYKKC 60

Query: 61  MEQRFKEAVASKGLEAAQVEVKDMDWESTFFLRHLPESNIAEMSELDEEYKEIMKEFAKK 120
           MEQRFKEAVASKGLEAAQ EVKDMDWESTFFLRHLPESNIAEMSELDE+YKEIMKEFAKK
Sbjct: 61  MEQRFKEAVASKGLEAAQDEVKDMDWESTFFLRHLPESNIAEMSELDEKYKEIMKEFAKK 120

Query: 121 IENLSEELLDLLCENLGLEKGYLKKVFHGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDA 180
           IENLSEELLDLLCE+LGLEKGYLKKVFHGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDA
Sbjct: 121 IENLSEELLDLLCEDLGLEKGYLKKVFHGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDA 180

Query: 181 GGIILLFQDNKVSGLQLLKDGNWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVMTQT 240
           GGIILLFQD+KVSGLQLLKDGNWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVMTQT
Sbjct: 181 GGIILLFQDDKVSGLQLLKDGNWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVMTQT 240

Query: 241 SGGGRMSLASFYNPGSDAVIFPAPSLI--EGEGEEKKEVYPKFVFEDYMKLYLGVKFEAK 300
           SGGGRMSLASFYNPGSDAVIFPAPSLI  EGEGEEKKEVYPKFVFEDYMKLYLGVKFEAK
Sbjct: 241 SGGGRMSLASFYNPGSDAVIFPAPSLIEKEGEGEEKKEVYPKFVFEDYMKLYLGVKFEAK 300

Query: 301 EPRFEAMKGNANLGPMATA 318
           EPRFEAMK NANLGPMATA
Sbjct: 301 EPRFEAMKANANLGPMATA 319

BLAST of Carg04642-RA vs. TrEMBL
Match: tr|D0V4H6|D0V4H6_CUCME (1-aminocyclopropane-1-carboxylate oxidase 1 OS=Cucumis melo OX=3656 GN=LOC103491357 PE=2 SV=1)

HSP 1 Score: 599.7 bits (1545), Expect = 3.7e-168
Identity = 287/318 (90.25%), Postives = 306/318 (96.23%), Query Frame = 0

Query: 1   MAIFPVINLENINGDGRAKILEQIEDACQNWGFFELVNHGIPHEFLDTVEKMTKDHYKKC 60
           MA+FP+INLENIN DGRAKILEQIEDACQNWGFFELVNHGIPHEFLD VEKMT+DHYKKC
Sbjct: 1   MAVFPIINLENINDDGRAKILEQIEDACQNWGFFELVNHGIPHEFLDMVEKMTRDHYKKC 60

Query: 61  MEQRFKEAVASKGLEAAQVEVKDMDWESTFFLRHLPESNIAEMSELDEEYKEIMKEFAKK 120
           ME+RFKE V SKGLEAAQ EV DMDWESTFFLRHLPESNI++MS+LDEEYK+IMKEFAKK
Sbjct: 61  MEERFKETVLSKGLEAAQAEVNDMDWESTFFLRHLPESNISQMSDLDEEYKKIMKEFAKK 120

Query: 121 IENLSEELLDLLCENLGLEKGYLKKVFHGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDA 180
           +ENL+EELLDLLCENLGLEKGYLKK F+GSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDA
Sbjct: 121 LENLAEELLDLLCENLGLEKGYLKKAFYGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDA 180

Query: 181 GGIILLFQDNKVSGLQLLKDGNWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVMTQT 240
           GGIILLFQD+KVSGLQLLKDGNWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRV+TQT
Sbjct: 181 GGIILLFQDDKVSGLQLLKDGNWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVLTQT 240

Query: 241 SGGGRMSLASFYNPGSDAVIFPAPSLIE-GEGEEKKEVYPKFVFEDYMKLYLGVKFEAKE 300
           SG GRMS+ASFYNPGSDAVI+PAP+L+E  + EEKKEVYPKFVFEDYMKLYLGVKF+AKE
Sbjct: 241 SGTGRMSIASFYNPGSDAVIYPAPALVEKDQDEEKKEVYPKFVFEDYMKLYLGVKFQAKE 300

Query: 301 PRFEAMKGNANLGPMATA 318
           PRFEAMK NANLGPMATA
Sbjct: 301 PRFEAMKANANLGPMATA 318

BLAST of Carg04642-RA vs. TrEMBL
Match: tr|C8KIS1|C8KIS1_CUCME (ACC oxidase OS=Cucumis melo OX=3656 GN=CM-ACO1 PE=3 SV=1)

HSP 1 Score: 598.6 bits (1542), Expect = 8.2e-168
Identity = 286/318 (89.94%), Postives = 306/318 (96.23%), Query Frame = 0

Query: 1   MAIFPVINLENINGDGRAKILEQIEDACQNWGFFELVNHGIPHEFLDTVEKMTKDHYKKC 60
           MA+FP+INLENIN DGRAKILEQIEDACQNWGFFELVNHGIPHEFLD VEKMT+DHYKKC
Sbjct: 1   MAVFPIINLENINDDGRAKILEQIEDACQNWGFFELVNHGIPHEFLDMVEKMTRDHYKKC 60

Query: 61  MEQRFKEAVASKGLEAAQVEVKDMDWESTFFLRHLPESNIAEMSELDEEYKEIMKEFAKK 120
           ME+RFKE V SKGLEAAQ EV DMDWESTFFLRHLPESNI++MS+LDEEYK+IMKEFAKK
Sbjct: 61  MEERFKETVLSKGLEAAQAEVNDMDWESTFFLRHLPESNISQMSDLDEEYKKIMKEFAKK 120

Query: 121 IENLSEELLDLLCENLGLEKGYLKKVFHGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDA 180
           +ENL+EELLDLLCENLGLEKGYLKK F+GSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDA
Sbjct: 121 LENLAEELLDLLCENLGLEKGYLKKAFYGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDA 180

Query: 181 GGIILLFQDNKVSGLQLLKDGNWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVMTQT 240
           GGIILLFQD+KVSGLQLLKDGNWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRV+TQT
Sbjct: 181 GGIILLFQDDKVSGLQLLKDGNWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVLTQT 240

Query: 241 SGGGRMSLASFYNPGSDAVIFPAPSLIE-GEGEEKKEVYPKFVFEDYMKLYLGVKFEAKE 300
           SG GRMS+ASFYNPGSDAVI+PAP+++E  + EEKKEVYPKFVFEDYMKLYLGVKF+AKE
Sbjct: 241 SGTGRMSIASFYNPGSDAVIYPAPAVVEKDQDEEKKEVYPKFVFEDYMKLYLGVKFQAKE 300

Query: 301 PRFEAMKGNANLGPMATA 318
           PRFEAMK NANLGPMATA
Sbjct: 301 PRFEAMKANANLGPMATA 318

BLAST of Carg04642-RA vs. TrEMBL
Match: tr|A0A097BU17|A0A097BU17_CITLA (1-aminocyclopropane-1-carboxylate oxidase 1 OS=Citrullus lanatus OX=3654 PE=2 SV=1)

HSP 1 Score: 598.2 bits (1541), Expect = 1.1e-167
Identity = 285/318 (89.62%), Postives = 306/318 (96.23%), Query Frame = 0

Query: 1   MAIFPVINLENINGDGRAKILEQIEDACQNWGFFELVNHGIPHEFLDTVEKMTKDHYKKC 60
           MA+FP+INLENINGDGR KILEQIEDACQNWGFFELVNHGIPHEFLDTVEKMT+DHYKKC
Sbjct: 1   MAVFPIINLENINGDGRVKILEQIEDACQNWGFFELVNHGIPHEFLDTVEKMTRDHYKKC 60

Query: 61  MEQRFKEAVASKGLEAAQVEVKDMDWESTFFLRHLPESNIAEMSELDEEYKEIMKEFAKK 120
           ME+RFKE V SKGLEAAQ EV DMDWESTFFLRHLPESNI+EMS+LDEEYK++MKEFAKK
Sbjct: 61  MEERFKETVLSKGLEAAQAEVDDMDWESTFFLRHLPESNISEMSDLDEEYKKVMKEFAKK 120

Query: 121 IENLSEELLDLLCENLGLEKGYLKKVFHGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDA 180
           +ENL+EELLDLLCENLGLEKGYLKK F+GSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDA
Sbjct: 121 LENLAEELLDLLCENLGLEKGYLKKAFYGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDA 180

Query: 181 GGIILLFQDNKVSGLQLLKDGNWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVMTQT 240
           GGIILLFQD+KVSGLQLLKD NWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRV+TQT
Sbjct: 181 GGIILLFQDDKVSGLQLLKDDNWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVITQT 240

Query: 241 SGGGRMSLASFYNPGSDAVIFPAPSLIE-GEGEEKKEVYPKFVFEDYMKLYLGVKFEAKE 300
           SG GR+S+ASFYNPGSDAVIFPAP+L+E  + EEKKEVYPKFVFEDYMKLY+GVKF+AKE
Sbjct: 241 SGTGRLSIASFYNPGSDAVIFPAPALVETDQAEEKKEVYPKFVFEDYMKLYIGVKFQAKE 300

Query: 301 PRFEAMKGNANLGPMATA 318
           PRFEAMK NA+LGPMATA
Sbjct: 301 PRFEAMKANASLGPMATA 318

BLAST of Carg04642-RA vs. TrEMBL
Match: tr|O82126|O82126_CUCSA (ACC oxidase OS=Cucumis sativus OX=3659 GN=CS-ACO1 PE=2 SV=1)

HSP 1 Score: 586.3 bits (1510), Expect = 4.2e-164
Identity = 283/318 (88.99%), Postives = 303/318 (95.28%), Query Frame = 0

Query: 1   MAIFPVINLENINGDGRAKILEQIEDACQNWGFFELVNHGIPHEFLDTVEKMTKDHYKKC 60
           MA+FP+INLENINGDGRAKILEQIEDACQNWGFFELVNHGIPHEFLD VEKMT+DH KKC
Sbjct: 1   MAVFPIINLENINGDGRAKILEQIEDACQNWGFFELVNHGIPHEFLDMVEKMTRDHDKKC 60

Query: 61  MEQRFKEAVASKGLEAAQVEVKDMDWESTFFLRHLPESNIAEMSELDEEYKEIMKEFAKK 120
           ME+RFKE V SKGLEAAQ EV DMDWESTFFLRHLPESNI++MS+LDEEYK+IMKEFAKK
Sbjct: 61  MEERFKETVLSKGLEAAQAEVNDMDWESTFFLRHLPESNISQMSDLDEEYKKIMKEFAKK 120

Query: 121 IENLSEELLDLLCENLGLEKGYLKKVFHGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDA 180
           +ENL+EELLDLLCENLGLEKGYLK  F+GSKGPTFGTKVS YPPCPKPDLIKGLRAHTDA
Sbjct: 121 LENLAEELLDLLCENLGLEKGYLKNAFYGSKGPTFGTKVSKYPPCPKPDLIKGLRAHTDA 180

Query: 181 GGIILLFQDNKVSGLQLLKDGNWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVM-TQ 240
           GGIILLFQD+KV GLQLLKDG+WIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRV+ TQ
Sbjct: 181 GGIILLFQDDKVGGLQLLKDGDWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVLITQ 240

Query: 241 TSGGGRMSLASFYNPGSDAVIFPAPSLIEGEGEEKKEVYPKFVFEDYMKLYLGVKFEAKE 300
           TSG GRMS+ASFYNPGSDAVI+PAP+L+E + EEKKEVYPKFVFEDYMKLYLGVKF+AKE
Sbjct: 241 TSGTGRMSIASFYNPGSDAVIYPAPALVE-KDEEKKEVYPKFVFEDYMKLYLGVKFQAKE 300

Query: 301 PRFEAMKGNANLGPMATA 318
           PRFEAMK NANLGPMATA
Sbjct: 301 PRFEAMKANANLGPMATA 317

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022930840.16.4e-18098.751-aminocyclopropane-1-carboxylate oxidase 1 [Cucurbita moschata][more]
XP_022988653.15.4e-17997.811-aminocyclopropane-1-carboxylate oxidase 1 [Cucurbita maxima][more]
XP_023529224.12.0e-17897.811-aminocyclopropane-1-carboxylate oxidase 1 [Cucurbita pepo subsp. pepo][more]
ADX66712.12.3e-17797.18ACO1 protein [Cucurbita pepo][more]
NP_001284392.15.6e-16890.251-aminocyclopropane-1-carboxylate oxidase 1 [Cucumis melo] >Q04644.1 RecName: Fu... [more]
Match NameE-valueIdentityDescription
AT1G05010.11.1e-14173.37ethylene-forming enzyme[more]
AT1G62380.11.1e-13069.31ACC oxidase 2[more]
AT1G12010.19.6e-13067.942-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein[more]
AT1G77330.15.3e-8049.372-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein[more]
AT2G19590.11.8e-7544.66ACC oxidase 1[more]
Match NameE-valueIdentityDescription
sp|Q04644|ACCO1_CUCME1.8e-17090.251-aminocyclopropane-1-carboxylate oxidase 1 OS=Cucumis melo OX=3656 GN=ACO1 PE=2... [more]
sp|P05116|ACCO1_SOLLC2.4e-14677.921-aminocyclopropane-1-carboxylate oxidase 1 OS=Solanum lycopersicum OX=4081 GN=A... [more]
sp|Q43792|ACCO_TOBAC3.1e-14677.191-aminocyclopropane-1-carboxylate oxidase OS=Nicotiana tabacum OX=4097 GN=ACO PE... [more]
sp|Q08507|ACCO3_PETHY5.4e-14676.951-aminocyclopropane-1-carboxylate oxidase 3 OS=Petunia hybrida OX=4102 GN=ACO3 P... [more]
sp|P24157|ACCO4_SOLLC7.0e-14680.391-aminocyclopropane-1-carboxylate oxidase 4 OS=Solanum lycopersicum OX=4081 GN=A... [more]
Match NameE-valueIdentityDescription
tr|H6TBX5|H6TBX5_CUCPE1.5e-17797.18ACO1 protein OS=Cucurbita pepo OX=3663 PE=2 SV=1[more]
tr|D0V4H6|D0V4H6_CUCME3.7e-16890.251-aminocyclopropane-1-carboxylate oxidase 1 OS=Cucumis melo OX=3656 GN=LOC103491... [more]
tr|C8KIS1|C8KIS1_CUCME8.2e-16889.94ACC oxidase OS=Cucumis melo OX=3656 GN=CM-ACO1 PE=3 SV=1[more]
tr|A0A097BU17|A0A097BU17_CITLA1.1e-16789.621-aminocyclopropane-1-carboxylate oxidase 1 OS=Citrullus lanatus OX=3654 PE=2 SV... [more]
tr|O82126|O82126_CUCSA4.2e-16488.99ACC oxidase OS=Cucumis sativus OX=3659 GN=CS-ACO1 PE=2 SV=1[more]
The following terms have been associated with this mRNA:
Vocabulary: Biological Process
TermDefinition
GO:0055114oxidation-reduction process
Vocabulary: Molecular Function
TermDefinition
GO:0016491oxidoreductase activity
Vocabulary: INTERPRO
TermDefinition
IPR005123Oxoglu/Fe-dep_dioxygenase
IPR027443IPNS-like
IPR026992DIOX_N
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055114 oxidation-reduction process
cellular_component GO:0005575 cellular_component
molecular_function GO:0016491 oxidoreductase activity

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Carg04642Carg04642gene


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Carg04642-RACarg04642-RA-proteinpolypeptide


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg04642-RA.exon.1Carg04642-RA.exon.1exon
Carg04642-RA.exon.2Carg04642-RA.exon.2exon
Carg04642-RA.exon.3Carg04642-RA.exon.3exon
Carg04642-RA.exon.4Carg04642-RA.exon.4exon


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg04642-RA.five_prime_UTR.1Carg04642-RA.five_prime_UTR.1five_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg04642-RA.CDS.1Carg04642-RA.CDS.1CDS
Carg04642-RA.CDS.2Carg04642-RA.CDS.2CDS
Carg04642-RA.CDS.3Carg04642-RA.CDS.3CDS
Carg04642-RA.CDS.4Carg04642-RA.CDS.4CDS


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg04642-RA.three_prime_UTR.1Carg04642-RA.three_prime_UTR.1three_prime_UTR


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 103..134
NoneNo IPR availablePANTHERPTHR10209:SF1931-AMINOCYCLOPROPANE-1-CARBOXYLATE OXIDASE 2-RELATEDcoord: 1..308
NoneNo IPR availablePANTHERPTHR10209OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEINcoord: 1..308
NoneNo IPR availableSUPERFAMILYSSF51197Clavaminate synthase-likecoord: 4..304
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 5..96
e-value: 2.8E-14
score: 53.8
IPR027443Isopenicillin N synthase-likeGENE3DG3DSA:2.60.120.330coord: 2..315
e-value: 1.1E-121
score: 408.1
IPR005123Oxoglutarate/iron-dependent dioxygenasePFAMPF031712OG-FeII_Oxycoord: 159..254
e-value: 6.5E-28
score: 97.1
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 153..254
score: 14.065