Carg01986-RA (mRNA) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg01986-RA
Type	mRNA
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	E3 ubiquitin-protein ligase
Location	Cucurbita_argyrosperma_scaffold_003 : 1542144 .. 1544153 (+)
Sequence length	958

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GCATTTTATTGATAAAAATGTCATCTGCAAATCCTTATTTTGATGACGTTCGAACCAAACCTGAGGTTATTGATCCTCCACAAGATGATGACATGATGGATGTTAGTGAAAGTGTGAGTGATCCTGCTCGAAATGGAGGAAAGCCTAACGTGGCTGTCTCGAGCAGTGTTCGTGACCTGTTGGAGTGCCCTGTTTGCTTAAATGCTATGTACCCTCCTATTCATCAGGTCAGTTTTCATCCTTCTAAGATCTTTTGAATGAAATTGTTTACTTGTTGGAGATGAACGCTTTGATACCAAATGATAAAGAATCAGTATAAGAGAAGTATGATTCAGATTAGCTTGTTGATCGAATATCTTAAGGCAAGAACACTTGTTTAGGTGAAGTAGGTGGTGTCAATGGTGGAGTTGGTGGTGATGTTAGTTATGGCAAAGGTTTTGGTGATGGGTTTGGCAGAGGTGGATTTGGTGGTGAGGTGGGTAGGGGATGTCGTTGGAGGCAGAAAATGGACGTTCGGCATCCCGAAACTTCGAGAACTCTTATTAGTTATACCATATTATATACACTTTGTTATTATTACAACATGAAACTGTTCTCATGGTCATGGAAGAGATTACATATATATTTGTTACGAGAACTAAAACGGATTCCCTATAAGTAATATAACCCCGAATCTCCTAAAAACTCCCGAACTCTCTTCAAAGATCGTATGAACGGTGATTCTTTCCATTCCGTAGCTCACGTAGAATTTGTGAGATCTCACATCGATTAGAGAGGGGAACGAGTACCAGTGAGGATATTGGGTTCCGAAGGGGGGGTGAATTGTGAGATCCCACATTGGTTGGGGAGGAGGAGAACGAAGCATTTTTTATAAGGGTGTGGAAATCTCTCCCTAGTTGACGCGTTTTAAAAACCTTGAGGGGAAGTCCGAAAGGGAAAACTCACAGATGGCAGTATTTGCTAACGGTAGTCATAACGGTAGGCTGGACTATTACAAACATAGATAGATGATTTTGGCTCGTTCCTATACAAAAGACACCGGCTTCGTGTGGATTAGAATAGCTCGAGAGCTGTCTTCCTTTTAGCCTCAACTGAATGTTCTCCTTGAGTTTATGAATAATGTTAAAGATTTTGGGTAGTCTTTGAGAGTACCATGTGTTCTTATGTCGATTAACTTTCTGTTGTCGTAGTGTTCGAACGGTCACACATTGTGTTCGGGATGCAAGCCGAGGGTGCACAACCGGTGTCCGACCTGCAGGCATGAACTCGGGAATATCAGATGTCTTGCATTAGAGAAGGTGGCTGCATCACTGGAACTTCCATGTAAATATCAAACTTTTGGATGTGTGGGCATATACCCTTACTATAGCAAGCTGAAACATGAATCTCAATGTGTATATCGACCCTATAACTGTCCTTATGCGGGGTCGGAATGCACGGTTGTTGGCGATATTCCGTTTTTGGTAGCTCACCTCAAGGACGATCACAAAGTCGACATGCATAATGGAAGCACTTTCAATCACCGGTACGTCAAGTCAAATCCGCAAGAGGTCGAAAATGCCACGTGGATGTTAACGGTACTATTCTGCTTCGTCATATTGTGTCATGTTTTGAGAGGTCGGACACGCACGCAACTGATACCGTTTTTTTTCTGTTTGATCGCTAGGTTTTCAGCTGTTTTGGTCAATACTTTTGTTTACATTTCGAAGCTTTTCAGCTCGGGATGGCACCGGTGTACATAGCTTTCTTGCGGTTTATGGGGGACGATAATGAAGCGAAGAACTACAGTTATAGTCTAGAGGTGGGTGGAAATGGGAGGAAGACAGTGTGGCAGGGAGTGCCACGGAGTATTCGGGACAGTCATCGGAAGGTACGAGACAGCTTTGACGGGCTTATTATCCAACGTAACATGGCGCTGTTCTTCTCGGGGGGAGATCGGAAAGAATTGAAGCTGAGGGTAACTGGTAGGATTTGGAAGGAGCAGTAGCAGTAGCAGTAGCAGTAATGTGT

mRNA sequence

GCATTTTATTGATAAAAATGTCATCTGCAAATCCTTATTTTGATGACGTTCGAACCAAACCTGAGGTTATTGATCCTCCACAAGATGATGACATGATGGATGTTAGTGAAAGTGTGAGTGATCCTGCTCGAAATGGAGGAAAGCCTAACGTGGCTGTCTCGAGCAGTGTTCGTGACCTGTTGGAGTGCCCTGTTTGCTTAAATGCTATGTACCCTCCTATTCATCAGTGTTCGAACGGTCACACATTGTGTTCGGGATGCAAGCCGAGGGTGCACAACCGGTGTCCGACCTGCAGGCATGAACTCGGGAATATCAGATGTCTTGCATTAGAGAAGGTGGCTGCATCACTGGAACTTCCATGTAAATATCAAACTTTTGGATGTGTGGGCATATACCCTTACTATAGCAAGCTGAAACATGAATCTCAATGTGTATATCGACCCTATAACTGTCCTTATGCGGGGTCGGAATGCACGGTTGTTGGCGATATTCCGTTTTTGGTAGCTCACCTCAAGGACGATCACAAAGTCGACATGCATAATGGAAGCACTTTCAATCACCGGTACGTCAAGTCAAATCCGCAAGAGGTCGAAAATGCCACGTGGATGTTAACGGTTTTCAGCTGTTTTGGTCAATACTTTTGTTTACATTTCGAAGCTTTTCAGCTCGGGATGGCACCGGTGTACATAGCTTTCTTGCGGTTTATGGGGGACGATAATGAAGCGAAGAACTACAGTTATAGTCTAGAGGTGGGTGGAAATGGGAGGAAGACAGTGTGGCAGGGAGTGCCACGGAGTATTCGGGACAGTCATCGGAAGGTACGAGACAGCTTTGACGGGCTTATTATCCAACGTAACATGGCGCTGTTCTTCTCGGGGGGAGATCGGAAAGAATTGAAGCTGAGGGTAACTGGTAGGATTTGGAAGGAGCAGTAGCAGTAGCAGTAGCAGTAATGTGT

Coding sequence (CDS)

ATGTCATCTGCAAATCCTTATTTTGATGACGTTCGAACCAAACCTGAGGTTATTGATCCTCCACAAGATGATGACATGATGGATGTTAGTGAAAGTGTGAGTGATCCTGCTCGAAATGGAGGAAAGCCTAACGTGGCTGTCTCGAGCAGTGTTCGTGACCTGTTGGAGTGCCCTGTTTGCTTAAATGCTATGTACCCTCCTATTCATCAGTGTTCGAACGGTCACACATTGTGTTCGGGATGCAAGCCGAGGGTGCACAACCGGTGTCCGACCTGCAGGCATGAACTCGGGAATATCAGATGTCTTGCATTAGAGAAGGTGGCTGCATCACTGGAACTTCCATGTAAATATCAAACTTTTGGATGTGTGGGCATATACCCTTACTATAGCAAGCTGAAACATGAATCTCAATGTGTATATCGACCCTATAACTGTCCTTATGCGGGGTCGGAATGCACGGTTGTTGGCGATATTCCGTTTTTGGTAGCTCACCTCAAGGACGATCACAAAGTCGACATGCATAATGGAAGCACTTTCAATCACCGGTACGTCAAGTCAAATCCGCAAGAGGTCGAAAATGCCACGTGGATGTTAACGGTTTTCAGCTGTTTTGGTCAATACTTTTGTTTACATTTCGAAGCTTTTCAGCTCGGGATGGCACCGGTGTACATAGCTTTCTTGCGGTTTATGGGGGACGATAATGAAGCGAAGAACTACAGTTATAGTCTAGAGGTGGGTGGAAATGGGAGGAAGACAGTGTGGCAGGGAGTGCCACGGAGTATTCGGGACAGTCATCGGAAGGTACGAGACAGCTTTGACGGGCTTATTATCCAACGTAACATGGCGCTGTTCTTCTCGGGGGGAGATCGGAAAGAATTGAAGCTGAGGGTAACTGGTAGGATTTGGAAGGAGCAGTAG

Protein sequence

MSSANPYFDDVRTKPEVIDPPQDDDMMDVSESVSDPARNGGKPNVAVSSSVRDLLECPVCLNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCKYQTFGCVGIYPYYSKLKHESQCVYRPYNCPYAGSECTVVGDIPFLVAHLKDDHKVDMHNGSTFNHRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLRFMGDDNEAKNYSYSLEVGGNGRKTVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGRIWKEQ

Homology

[Close]

BLAST of Carg01986-RA vs. NCBI nr
Match: XP_023524871.1 (E3 ubiquitin-protein ligase DIS1 [Cucurbita pepo subsp. pepo] >XP_023524879.1 E3 ubiquitin-protein ligase DIS1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 650.2 bits (1676), Expect = 3.5e-183
Identity = 304/305 (99.67%), Postives = 305/305 (100.00%), Query Frame = 0


Query: 1   MSSANPYFDDVRTKPEVIDPPQDDDMMDVSESVSDPARNGGKPNVAVSSSVRDLLECPVC 60
           MSSANPYFDDVRTKPEVIDPPQDDDMMDVSESVSDPARNGGKPNVAVSSSVR+LLECPVC
Sbjct: 1   MSSANPYFDDVRTKPEVIDPPQDDDMMDVSESVSDPARNGGKPNVAVSSSVRELLECPVC 60

Query: 61  LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCKYQTF 120
           LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCKYQTF
Sbjct: 61  LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCKYQTF 120

Query: 121 GCVGIYPYYSKLKHESQCVYRPYNCPYAGSECTVVGDIPFLVAHLKDDHKVDMHNGSTFN 180
           GCVGIYPYYSKLKHESQCVYRPYNCPYAGSECTVVGDIPFLVAHLKDDHKVDMHNGSTFN
Sbjct: 121 GCVGIYPYYSKLKHESQCVYRPYNCPYAGSECTVVGDIPFLVAHLKDDHKVDMHNGSTFN 180

Query: 181 HRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLRFMGDDNEAKNYS 240
           HRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLRFMGDDNEAKNYS
Sbjct: 181 HRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLRFMGDDNEAKNYS 240

Query: 241 YSLEVGGNGRKTVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR 300
           YSLEVGGNGRKTVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR
Sbjct: 241 YSLEVGGNGRKTVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR 300

Query: 301 IWKEQ 306
           IWKEQ
Sbjct: 301 IWKEQ 305

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BLAST of Carg01986-RA vs. NCBI nr
Match: XP_022956691.1 (E3 ubiquitin-protein ligase DIS1 [Cucurbita moschata] >XP_022956692.1 E3 ubiquitin-protein ligase DIS1 [Cucurbita moschata])

HSP 1 Score: 647.5 bits (1669), Expect = 2.3e-182
Identity = 303/305 (99.34%), Postives = 304/305 (99.67%), Query Frame = 0


Query: 1   MSSANPYFDDVRTKPEVIDPPQDDDMMDVSESVSDPARNGGKPNVAVSSSVRDLLECPVC 60
           MSSANPYFDDVRTKPEVIDPPQDDDMMDVSESVSDPARNGGKPNVAVSSSVR+LLECPVC
Sbjct: 1   MSSANPYFDDVRTKPEVIDPPQDDDMMDVSESVSDPARNGGKPNVAVSSSVRELLECPVC 60

Query: 61  LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCKYQTF 120
           LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCKY TF
Sbjct: 61  LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCKYLTF 120

Query: 121 GCVGIYPYYSKLKHESQCVYRPYNCPYAGSECTVVGDIPFLVAHLKDDHKVDMHNGSTFN 180
           GCVGIYPYYSKLKHESQCVYRPYNCPYAGSECTVVGDIPFLVAHLKDDHKVDMHNGSTFN
Sbjct: 121 GCVGIYPYYSKLKHESQCVYRPYNCPYAGSECTVVGDIPFLVAHLKDDHKVDMHNGSTFN 180

Query: 181 HRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLRFMGDDNEAKNYS 240
           HRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLRFMGDDNEAKNYS
Sbjct: 181 HRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLRFMGDDNEAKNYS 240

Query: 241 YSLEVGGNGRKTVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR 300
           YSLEVGGNGRKTVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR
Sbjct: 241 YSLEVGGNGRKTVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR 300

Query: 301 IWKEQ 306
           IWKEQ
Sbjct: 301 IWKEQ 305

[Close]

BLAST of Carg01986-RA vs. NCBI nr
Match: XP_022992030.1 (E3 ubiquitin-protein ligase DIS1 [Cucurbita maxima])

HSP 1 Score: 646.0 bits (1665), Expect = 6.6e-182
Identity = 302/305 (99.02%), Postives = 303/305 (99.34%), Query Frame = 0


Query: 1   MSSANPYFDDVRTKPEVIDPPQDDDMMDVSESVSDPARNGGKPNVAVSSSVRDLLECPVC 60
           MSSANPYFDDVRTKPEVIDPPQDDDMMDVSESVSDPARNGGKPNV VSSSVR+LLECPVC
Sbjct: 1   MSSANPYFDDVRTKPEVIDPPQDDDMMDVSESVSDPARNGGKPNVVVSSSVRELLECPVC 60

Query: 61  LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCKYQTF 120
           LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCKYQTF
Sbjct: 61  LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCKYQTF 120

Query: 121 GCVGIYPYYSKLKHESQCVYRPYNCPYAGSECTVVGDIPFLVAHLKDDHKVDMHNGSTFN 180
           GCVGIYPYYSKLKHESQCVYRPYNCPYAGSECTVVGDIPFLVAHLKDDHKVDMHNGSTFN
Sbjct: 121 GCVGIYPYYSKLKHESQCVYRPYNCPYAGSECTVVGDIPFLVAHLKDDHKVDMHNGSTFN 180

Query: 181 HRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLRFMGDDNEAKNYS 240
           HRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLRFMGDDNEAKNYS
Sbjct: 181 HRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLRFMGDDNEAKNYS 240

Query: 241 YSLEVGGNGRKTVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR 300
           YSLEVGGNGRKTVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGD KELKLRVTGR
Sbjct: 241 YSLEVGGNGRKTVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDGKELKLRVTGR 300

Query: 301 IWKEQ 306
           IWKEQ
Sbjct: 301 IWKEQ 305

[Close]

BLAST of Carg01986-RA vs. NCBI nr
Match: XP_022139330.1 (E3 ubiquitin-protein ligase SINAT5-like [Momordica charantia])

HSP 1 Score: 637.5 bits (1643), Expect = 2.3e-179
Identity = 296/305 (97.05%), Postives = 301/305 (98.69%), Query Frame = 0


Query: 1   MSSANPYFDDVRTKPEVIDPPQDDDMMDVSESVSDPARNGGKPNVAVSSSVRDLLECPVC 60
           MSSANPYFDD+R+KPEVIDPP +DDMMDVSESVSDP+RNGGKPNVAVSSSVRDLLECPVC
Sbjct: 1   MSSANPYFDDIRSKPEVIDPPPNDDMMDVSESVSDPSRNGGKPNVAVSSSVRDLLECPVC 60

Query: 61  LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCKYQTF 120
           LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCKYQ+F
Sbjct: 61  LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCKYQSF 120

Query: 121 GCVGIYPYYSKLKHESQCVYRPYNCPYAGSECTVVGDIPFLVAHLKDDHKVDMHNGSTFN 180
           GCVGIYPYYSKLKHESQCVYRPYNCPYAGSECTVVGDIPFLV HLKDDHKVDMHNGSTFN
Sbjct: 121 GCVGIYPYYSKLKHESQCVYRPYNCPYAGSECTVVGDIPFLVTHLKDDHKVDMHNGSTFN 180

Query: 181 HRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLRFMGDDNEAKNYS 240
           HRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLRFMGDDNEAKNYS
Sbjct: 181 HRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLRFMGDDNEAKNYS 240

Query: 241 YSLEVGGNGRKTVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR 300
           YSLEVGGNGRK VWQGVPRSIRD HRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR
Sbjct: 241 YSLEVGGNGRKMVWQGVPRSIRDGHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR 300

Query: 301 IWKEQ 306
           IWKEQ
Sbjct: 301 IWKEQ 305

[Close]

BLAST of Carg01986-RA vs. NCBI nr
Match: XP_023541838.1 (E3 ubiquitin-protein ligase DIS1-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 637.5 bits (1643), Expect = 2.3e-179
Identity = 297/305 (97.38%), Postives = 299/305 (98.03%), Query Frame = 0


Query: 1   MSSANPYFDDVRTKPEVIDPPQDDDMMDVSESVSDPARNGGKPNVAVSSSVRDLLECPVC 60
           MSSANPYFDDVRTKPEVIDPPQD+DMMDVSESVSDP RNGGKPNVAVSSSVR+LLECPVC
Sbjct: 1   MSSANPYFDDVRTKPEVIDPPQDEDMMDVSESVSDPTRNGGKPNVAVSSSVRELLECPVC 60

Query: 61  LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCKYQTF 120
           LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPC YQTF
Sbjct: 61  LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCTYQTF 120

Query: 121 GCVGIYPYYSKLKHESQCVYRPYNCPYAGSECTVVGDIPFLVAHLKDDHKVDMHNGSTFN 180
           GC GIYPYYSKLKHESQC YRPYNCPYAGSECTVVGDIPFLVAHLKDDHKVDMHNGSTFN
Sbjct: 121 GCAGIYPYYSKLKHESQCAYRPYNCPYAGSECTVVGDIPFLVAHLKDDHKVDMHNGSTFN 180

Query: 181 HRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLRFMGDDNEAKNYS 240
           HRYVKSNPQEVENATWMLTVFSCFG YFCLHFEAFQLGMAPVYIAFLRFMGDDNEAKNYS
Sbjct: 181 HRYVKSNPQEVENATWMLTVFSCFGHYFCLHFEAFQLGMAPVYIAFLRFMGDDNEAKNYS 240

Query: 241 YSLEVGGNGRKTVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR 300
           YSLEVGGNGRK VWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR
Sbjct: 241 YSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR 300

Query: 301 IWKEQ 306
           IWKEQ
Sbjct: 301 IWKEQ 305

[Close]

BLAST of Carg01986-RA vs. TAIR10
Match: AT3G61790.1 (Protein with RING/U-box and TRAF-like domains)

HSP 1 Score: 479.9 bits (1234), Expect = 1.1e-135
Identity = 215/256 (83.98%), Postives = 235/256 (91.80%), Query Frame = 0


Query: 50  SVRDLLECPVCLNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAA 109
           SV +LLECPVC N+MYPPIHQC NGHTLCS CK RVHNRCPTCR ELG+IRCLALEKVA 
Sbjct: 56  SVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKARVHNRCPTCRQELGDIRCLALEKVAE 115

Query: 110 SLELPCKYQTFGCVGIYPYYSKLKHESQCVYRPYNCPYAGSECTVVGDIPFLVAHLKDDH 169
           SLELPCK+ + GC  I+PYYSKLKHE+ C +RPY+CPYAGSEC+V GDIPFLVAHL+DDH
Sbjct: 116 SLELPCKHMSLGCPEIFPYYSKLKHETVCNFRPYSCPYAGSECSVTGDIPFLVAHLRDDH 175

Query: 170 KVDMHNGSTFNHRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLRF 229
           KVDMH+G TFNHRYVKSNP+EVENATWMLTVF CFGQYFCLHFEAFQLGMAPVY+AFLRF
Sbjct: 176 KVDMHSGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFLRF 235

Query: 230 MGDDNEAKNYSYSLEVGGNGRKTVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGD 289
           MGD+ EA+NY+YSLEVGG GRK +W+G PRS+RDSHRKVRDS DGLIIQRNMALFFSGGD
Sbjct: 236 MGDETEARNYNYSLEVGGYGRKLIWEGTPRSVRDSHRKVRDSHDGLIIQRNMALFFSGGD 295

Query: 290 RKELKLRVTGRIWKEQ 306
           RKELKLRVTGRIWKEQ
Sbjct: 296 RKELKLRVTGRIWKEQ 311

[Close]

BLAST of Carg01986-RA vs. TAIR10
Match: AT4G27880.1 (Protein with RING/U-box and TRAF-like domains)

HSP 1 Score: 474.2 bits (1219), Expect = 6.2e-134
Identity = 216/265 (81.51%), Postives = 236/265 (89.06%), Query Frame = 0


Query: 41  GKPNVAVSSSVRDLLECPVCLNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIR 100
           G    A ++SV +LLECPVC  +MYPPIHQC NGHTLCS CK RVHNRCPTCR ELG+IR
Sbjct: 48  GPTATAPATSVYELLECPVCTYSMYPPIHQCHNGHTLCSTCKVRVHNRCPTCRQELGDIR 107

Query: 101 CLALEKVAASLELPCKYQTFGCVGIYPYYSKLKHESQCVYRPYNCPYAGSECTVVGDIPF 160
           CLALEKVA SLELPCK+   GC  I+PYYSKLKHES C +RPY+CPYAGSEC +VGDIPF
Sbjct: 108 CLALEKVAESLELPCKFYNLGCPEIFPYYSKLKHESLCNFRPYSCPYAGSECGIVGDIPF 167

Query: 161 LVAHLKDDHKVDMHNGSTFNHRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMA 220
           LVAHL+DDHKVDMH GSTFNHRYVKSNP+EVENATWMLTVF CFGQYFCLHFEAFQLGM 
Sbjct: 168 LVAHLRDDHKVDMHAGSTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMG 227

Query: 221 PVYIAFLRFMGDDNEAKNYSYSLEVGGNGRKTVWQGVPRSIRDSHRKVRDSFDGLIIQRN 280
           PVY+AFLRFMGD+ +A++YSYSLEVGG+GRK  W+G PRSIRDSHRKVRDS DGLIIQRN
Sbjct: 228 PVYMAFLRFMGDEEDARSYSYSLEVGGSGRKLTWEGTPRSIRDSHRKVRDSNDGLIIQRN 287

Query: 281 MALFFSGGDRKELKLRVTGRIWKEQ 306
           MALFFSGGDRKELKLRVTG+IWKEQ
Sbjct: 288 MALFFSGGDRKELKLRVTGKIWKEQ 312

[Close]

BLAST of Carg01986-RA vs. TAIR10
Match: AT3G58040.1 (seven in absentia of Arabidopsis 2)

HSP 1 Score: 463.8 bits (1192), Expect = 8.4e-131
Identity = 209/265 (78.87%), Postives = 231/265 (87.17%), Query Frame = 0


Query: 41  GKPNVAVSSSVRDLLECPVCLNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIR 100
           GK  +  ++ V +LLECPVC N MYPPIHQC NGHTLCS CK RV N CPTCR+ELGNIR
Sbjct: 44  GKYGIHSNNGVYELLECPVCTNLMYPPIHQCPNGHTLCSNCKLRVQNTCPTCRYELGNIR 103

Query: 101 CLALEKVAASLELPCKYQTFGCVGIYPYYSKLKHESQCVYRPYNCPYAGSECTVVGDIPF 160
           CLALEKVA SLE+PC+YQ  GC  I+PYYSKLKHE  C +RPY CPYAGSEC+V GDIP 
Sbjct: 104 CLALEKVAESLEVPCRYQNLGCHDIFPYYSKLKHEQHCRFRPYTCPYAGSECSVTGDIPT 163

Query: 161 LVAHLKDDHKVDMHNGSTFNHRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMA 220
           LV HLKDDHKVDMH+G TFNHRYVKSNP EVENATWMLTVF+CFG+ FCLHFEAFQLGMA
Sbjct: 164 LVVHLKDDHKVDMHDGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMA 223

Query: 221 PVYIAFLRFMGDDNEAKNYSYSLEVGGNGRKTVWQGVPRSIRDSHRKVRDSFDGLIIQRN 280
           PVY+AFLRFMGD+NEAK +SYSLEVG +GRK  WQG+PRSIRDSHRKVRDS DGLII RN
Sbjct: 224 PVYMAFLRFMGDENEAKKFSYSLEVGAHGRKLTWQGIPRSIRDSHRKVRDSQDGLIIPRN 283

Query: 281 MALFFSGGDRKELKLRVTGRIWKEQ 306
           +AL+FSGGDR+ELKLRVTGRIWKE+
Sbjct: 284 LALYFSGGDRQELKLRVTGRIWKEE 308

[Close]

BLAST of Carg01986-RA vs. TAIR10
Match: AT2G41980.1 (Protein with RING/U-box and TRAF-like domains)

HSP 1 Score: 453.4 bits (1165), Expect = 1.1e-127
Identity = 208/277 (75.09%), Postives = 233/277 (84.12%), Query Frame = 0


Query: 31  ESVSDPARNGGKP--NVAVSSSVRDLLECPVCLNAMYPPIHQCSNGHTLCSGCKPRVHNR 90
           E+ S P ++G         S+ V +LLECPVC N MYPPIHQC NGHTLCS CK RV N 
Sbjct: 29  EANSKPTKSGSGSIGKFHSSNGVYELLECPVCTNLMYPPIHQCPNGHTLCSSCKLRVQNT 88

Query: 91  CPTCRHELGNIRCLALEKVAASLELPCKYQTFGCVGIYPYYSKLKHESQCVYRPYNCPYA 150
           CPTCR+ELGNIRCLALEKVA SLE+PC+YQ  GC  I+PYYSKLKHE  C +R Y+CPYA
Sbjct: 89  CPTCRYELGNIRCLALEKVAESLEVPCRYQNLGCQDIFPYYSKLKHEQHCRFRSYSCPYA 148

Query: 151 GSECTVVGDIPFLVAHLKDDHKVDMHNGSTFNHRYVKSNPQEVENATWMLTVFSCFGQYF 210
           GSEC+V GDIP LV HLKDDHKVDMH+G TFNHRYVKSNP EVENATWMLTVF+CFG+ F
Sbjct: 149 GSECSVTGDIPTLVDHLKDDHKVDMHDGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQF 208

Query: 211 CLHFEAFQLGMAPVYIAFLRFMGDDNEAKNYSYSLEVGGNGRKTVWQGVPRSIRDSHRKV 270
           CLHFEAFQLGMAPVY+AFLRFMGD+NEAK +SYSLEVG + RK  WQG+PRSIRDSHRKV
Sbjct: 209 CLHFEAFQLGMAPVYMAFLRFMGDENEAKKFSYSLEVGAHSRKLTWQGIPRSIRDSHRKV 268

Query: 271 RDSFDGLIIQRNMALFFSGGDRKELKLRVTGRIWKEQ 306
           RDS DGLII RN+AL+FSG D++ELKLRVTGRIWKE+
Sbjct: 269 RDSQDGLIIPRNLALYFSGSDKEELKLRVTGRIWKEE 305

[Close]

BLAST of Carg01986-RA vs. TAIR10
Match: AT5G53360.1 (TRAF-like superfamily protein)

HSP 1 Score: 369.0 bits (946), Expect = 2.8e-102
Identity = 166/204 (81.37%), Postives = 183/204 (89.71%), Query Frame = 0


Query: 102 LALEKVAASLELPCKYQTFGCVGIYPYYSKLKHESQCVYRPYNCPYAGSECTVVGDIPFL 161
           +ALEKVA SLELPCKY   GC+GI+PYYSKLKHESQC +RPY+CPYAGSEC  VGDI FL
Sbjct: 15  IALEKVAESLELPCKYYNLGCLGIFPYYSKLKHESQCNFRPYSCPYAGSECAAVGDITFL 74

Query: 162 VAHLKDDHKVDMHNGSTFNHRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAP 221
           VAHL+DDHKVDMH G TFNHRYVKSNP+EVENATWMLTVF CFGQYFCLHFEAFQLGMAP
Sbjct: 75  VAHLRDDHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFQCFGQYFCLHFEAFQLGMAP 134

Query: 222 VYIAFLRFMGDDNEAKNYSYSLEVGGNGRKTVWQGVPRSIRDSHRKVRDSFDGLIIQRNM 281
           VY+AFLRFMGD+++A+NY+YSLEVGG+GRK  W+G PRS+RDSH       DGLIIQRNM
Sbjct: 135 VYMAFLRFMGDEDDARNYTYSLEVGGSGRKQTWEGTPRSVRDSHXXXXXXXDGLIIQRNM 194

Query: 282 ALFFSGGDRKELKLRVTGRIWKEQ 306
           ALFFSGGD+KELKLRVTGRIWKEQ
Sbjct: 195 ALFFSGGDKKELKLRVTGRIWKEQ 218

[Close]

BLAST of Carg01986-RA vs. Swiss-Prot
Match: sp|Q10L91|DIS1_ORYSJ (E3 ubiquitin-protein ligase DIS1 OS=Oryza sativa subsp. japonica OX=39947 GN=DIS1 PE=1 SV=1)

HSP 1 Score: 555.4 bits (1430), Expect = 3.8e-157
Identity = 260/299 (86.96%), Postives = 274/299 (91.64%), Query Frame = 0


Query: 7   YFDDVRTKPEVIDPPQDDDMMDVSESVSDPARNGGKPNVAVSSSVRDLLECPVCLNAMYP 66
           Y DD  +  EVIDPP+  +++DV+E   DP  +  KPNV VSSSVR+LLECPVCL+AMYP
Sbjct: 6   YIDD--SGSEVIDPPK-TEVLDVTELAGDPVPHSPKPNVVVSSSVRELLECPVCLSAMYP 65

Query: 67  PIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCKYQTFGCVGIY 126
           PIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCKYQ FGCVGIY
Sbjct: 66  PIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCKYQNFGCVGIY 125

Query: 127 PYYSKLKHESQCVYRPYNCPYAGSECTVVGDIPFLVAHLKDDHKVDMHNGSTFNHRYVKS 186
           PYY KLKHESQC YRPY+CPYAGSECTV GDIP+LV HLKDDHKVDMHNG TFNHRYVKS
Sbjct: 126 PYYCKLKHESQCQYRPYSCPYAGSECTVAGDIPYLVNHLKDDHKVDMHNGCTFNHRYVKS 185

Query: 187 NPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLRFMGDDNEAKNYSYSLEVG 246
           NP EVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLRFMGDD EAKNYSYSLEVG
Sbjct: 186 NPHEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLRFMGDDLEAKNYSYSLEVG 245

Query: 247 GNGRKTVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGRIWKEQ 306
           G GRK +WQGVPRSIRDSHRKVRDS+DGLIIQRNMALFFSGG+RKELKLRVTGRIWKEQ
Sbjct: 246 GTGRKMIWQGVPRSIRDSHRKVRDSYDGLIIQRNMALFFSGGERKELKLRVTGRIWKEQ 301

[Close]

BLAST of Carg01986-RA vs. Swiss-Prot
Match: sp|Q84JL3|SINA3_ARATH (E3 ubiquitin-protein ligase SINAT3 OS=Arabidopsis thaliana OX=3702 GN=SINAT3 PE=1 SV=1)

HSP 1 Score: 479.9 bits (1234), Expect = 2.0e-134
Identity = 215/256 (83.98%), Postives = 235/256 (91.80%), Query Frame = 0


Query: 50  SVRDLLECPVCLNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAA 109
           SV +LLECPVC N+MYPPIHQC NGHTLCS CK RVHNRCPTCR ELG+IRCLALEKVA 
Sbjct: 56  SVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKARVHNRCPTCRQELGDIRCLALEKVAE 115

Query: 110 SLELPCKYQTFGCVGIYPYYSKLKHESQCVYRPYNCPYAGSECTVVGDIPFLVAHLKDDH 169
           SLELPCK+ + GC  I+PYYSKLKHE+ C +RPY+CPYAGSEC+V GDIPFLVAHL+DDH
Sbjct: 116 SLELPCKHMSLGCPEIFPYYSKLKHETVCNFRPYSCPYAGSECSVTGDIPFLVAHLRDDH 175

Query: 170 KVDMHNGSTFNHRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLRF 229
           KVDMH+G TFNHRYVKSNP+EVENATWMLTVF CFGQYFCLHFEAFQLGMAPVY+AFLRF
Sbjct: 176 KVDMHSGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFLRF 235

Query: 230 MGDDNEAKNYSYSLEVGGNGRKTVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGD 289
           MGD+ EA+NY+YSLEVGG GRK +W+G PRS+RDSHRKVRDS DGLIIQRNMALFFSGGD
Sbjct: 236 MGDETEARNYNYSLEVGGYGRKLIWEGTPRSVRDSHRKVRDSHDGLIIQRNMALFFSGGD 295

Query: 290 RKELKLRVTGRIWKEQ 306
           RKELKLRVTGRIWKEQ
Sbjct: 296 RKELKLRVTGRIWKEQ 311

[Close]

BLAST of Carg01986-RA vs. Swiss-Prot
Match: sp|Q9STN8|SINA4_ARATH (E3 ubiquitin-protein ligase SINAT4 OS=Arabidopsis thaliana OX=3702 GN=SINAT4 PE=1 SV=1)

HSP 1 Score: 474.2 bits (1219), Expect = 1.1e-132
Identity = 216/265 (81.51%), Postives = 236/265 (89.06%), Query Frame = 0


Query: 41  GKPNVAVSSSVRDLLECPVCLNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIR 100
           G    A ++SV +LLECPVC  +MYPPIHQC NGHTLCS CK RVHNRCPTCR ELG+IR
Sbjct: 48  GPTATAPATSVYELLECPVCTYSMYPPIHQCHNGHTLCSTCKVRVHNRCPTCRQELGDIR 107

Query: 101 CLALEKVAASLELPCKYQTFGCVGIYPYYSKLKHESQCVYRPYNCPYAGSECTVVGDIPF 160
           CLALEKVA SLELPCK+   GC  I+PYYSKLKHES C +RPY+CPYAGSEC +VGDIPF
Sbjct: 108 CLALEKVAESLELPCKFYNLGCPEIFPYYSKLKHESLCNFRPYSCPYAGSECGIVGDIPF 167

Query: 161 LVAHLKDDHKVDMHNGSTFNHRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMA 220
           LVAHL+DDHKVDMH GSTFNHRYVKSNP+EVENATWMLTVF CFGQYFCLHFEAFQLGM 
Sbjct: 168 LVAHLRDDHKVDMHAGSTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMG 227

Query: 221 PVYIAFLRFMGDDNEAKNYSYSLEVGGNGRKTVWQGVPRSIRDSHRKVRDSFDGLIIQRN 280
           PVY+AFLRFMGD+ +A++YSYSLEVGG+GRK  W+G PRSIRDSHRKVRDS DGLIIQRN
Sbjct: 228 PVYMAFLRFMGDEEDARSYSYSLEVGGSGRKLTWEGTPRSIRDSHRKVRDSNDGLIIQRN 287

Query: 281 MALFFSGGDRKELKLRVTGRIWKEQ 306
           MALFFSGGDRKELKLRVTG+IWKEQ
Sbjct: 288 MALFFSGGDRKELKLRVTGKIWKEQ 312

[Close]

BLAST of Carg01986-RA vs. Swiss-Prot
Match: sp|Q8S3N1|SINA5_ARATH (E3 ubiquitin-protein ligase SINAT5 OS=Arabidopsis thaliana OX=3702 GN=SINAT5 PE=1 SV=2)

HSP 1 Score: 473.4 bits (1217), Expect = 1.9e-132
Identity = 219/289 (75.78%), Postives = 242/289 (83.74%), Query Frame = 0


Query: 18  IDPPQDDDMMDVSESVSDPARNGGKPNV-AVSSSVRDLLECPVCLNAMYPPIHQCSNGHT 77
           ID   DDD +      S     GG   V + ++SV +LLECPVC N+MYPPIHQC NGHT
Sbjct: 6   IDSVIDDDEIHQKHQFSSTKSQGGATVVISPATSVYELLECPVCTNSMYPPIHQCHNGHT 65

Query: 78  LCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCKYQTFGCVGIYPYYSKLKHES 137
           LCS CK RVHNRCPTCR ELG+IRCLALEKVA SLELPCKY   GC+GI+PYYSKLKHES
Sbjct: 66  LCSTCKSRVHNRCPTCRQELGDIRCLALEKVAESLELPCKYYNLGCLGIFPYYSKLKHES 125

Query: 138 QCVYRPYNCPYAGSECTVVGDIPFLVAHLKDDHKVDMHNGSTFNHRYVKSNPQEVENATW 197
           QC +RPY+CPYAGSEC  VGDI FLVAHL+DDHKVDMH G TFNHRYVKSNP+EVENATW
Sbjct: 126 QCNFRPYSCPYAGSECAAVGDITFLVAHLRDDHKVDMHTGCTFNHRYVKSNPREVENATW 185

Query: 198 MLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLRFMGDDNEAKNYSYSLEVGGNGRKTVWQG 257
           MLTVF CFGQYFCLHFEAFQLGMAPVY+AFLRFMGD+++A+NY+YSLEVGG+GRK  W+G
Sbjct: 186 MLTVFQCFGQYFCLHFEAFQLGMAPVYMAFLRFMGDEDDARNYTYSLEVGGSGRKQTWEG 245

Query: 258 VPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGRIWKEQ 306
            PRS+RDSH       DGLIIQRNMALFFSGGD+KELKLRVTGRIWKEQ
Sbjct: 246 TPRSVRDSHXXXXXXXDGLIIQRNMALFFSGGDKKELKLRVTGRIWKEQ 294

[Close]

BLAST of Carg01986-RA vs. Swiss-Prot
Match: sp|Q9M2P4|SINA2_ARATH (E3 ubiquitin-protein ligase SINAT2 OS=Arabidopsis thaliana OX=3702 GN=SINAT2 PE=1 SV=1)

HSP 1 Score: 463.8 bits (1192), Expect = 1.5e-129
Identity = 209/265 (78.87%), Postives = 231/265 (87.17%), Query Frame = 0


Query: 41  GKPNVAVSSSVRDLLECPVCLNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIR 100
           GK  +  ++ V +LLECPVC N MYPPIHQC NGHTLCS CK RV N CPTCR+ELGNIR
Sbjct: 44  GKYGIHSNNGVYELLECPVCTNLMYPPIHQCPNGHTLCSNCKLRVQNTCPTCRYELGNIR 103

Query: 101 CLALEKVAASLELPCKYQTFGCVGIYPYYSKLKHESQCVYRPYNCPYAGSECTVVGDIPF 160
           CLALEKVA SLE+PC+YQ  GC  I+PYYSKLKHE  C +RPY CPYAGSEC+V GDIP 
Sbjct: 104 CLALEKVAESLEVPCRYQNLGCHDIFPYYSKLKHEQHCRFRPYTCPYAGSECSVTGDIPT 163

Query: 161 LVAHLKDDHKVDMHNGSTFNHRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMA 220
           LV HLKDDHKVDMH+G TFNHRYVKSNP EVENATWMLTVF+CFG+ FCLHFEAFQLGMA
Sbjct: 164 LVVHLKDDHKVDMHDGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMA 223

Query: 221 PVYIAFLRFMGDDNEAKNYSYSLEVGGNGRKTVWQGVPRSIRDSHRKVRDSFDGLIIQRN 280
           PVY+AFLRFMGD+NEAK +SYSLEVG +GRK  WQG+PRSIRDSHRKVRDS DGLII RN
Sbjct: 224 PVYMAFLRFMGDENEAKKFSYSLEVGAHGRKLTWQGIPRSIRDSHRKVRDSQDGLIIPRN 283

Query: 281 MALFFSGGDRKELKLRVTGRIWKEQ 306
           +AL+FSGGDR+ELKLRVTGRIWKE+
Sbjct: 284 LALYFSGGDRQELKLRVTGRIWKEE 308

[Close]

BLAST of Carg01986-RA vs. TrEMBL
Match: tr|A0A1S3BGX1|A0A1S3BGX1_CUCME (E3 ubiquitin-protein ligase OS=Cucumis melo OX=3656 GN=LOC103489502 PE=3 SV=1)

HSP 1 Score: 632.9 bits (1631), Expect = 3.8e-178
Identity = 295/305 (96.72%), Postives = 301/305 (98.69%), Query Frame = 0


Query: 1   MSSANPYFDDVRTKPEVIDPPQDDDMMDVSESVSDPARNGGKPNVAVSSSVRDLLECPVC 60
           M+SANPYFDD+R KPEVIDPPQDDDMMDVSESVSDPAR GGKPNV VSSSVR+LLECPVC
Sbjct: 1   MTSANPYFDDIRNKPEVIDPPQDDDMMDVSESVSDPARAGGKPNV-VSSSVRELLECPVC 60

Query: 61  LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCKYQTF 120
           LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCKYQTF
Sbjct: 61  LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCKYQTF 120

Query: 121 GCVGIYPYYSKLKHESQCVYRPYNCPYAGSECTVVGDIPFLVAHLKDDHKVDMHNGSTFN 180
           GCVGIYPYYSKLKHESQC+YRPYNCPYAGSEC+V+GDIPFLVAHLKDDHKVDMHNGSTFN
Sbjct: 121 GCVGIYPYYSKLKHESQCIYRPYNCPYAGSECSVIGDIPFLVAHLKDDHKVDMHNGSTFN 180

Query: 181 HRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLRFMGDDNEAKNYS 240
           HRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLRFMGDDNEAKNYS
Sbjct: 181 HRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLRFMGDDNEAKNYS 240

Query: 241 YSLEVGGNGRKTVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR 300
           YSLEVGGNGRK VWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR
Sbjct: 241 YSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR 300

Query: 301 IWKEQ 306
           IWKEQ
Sbjct: 301 IWKEQ 304

[Close]

BLAST of Carg01986-RA vs. TrEMBL
Match: tr|E5GCK0|E5GCK0_CUCME (E3 ubiquitin-protein ligase OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1)

HSP 1 Score: 632.9 bits (1631), Expect = 3.8e-178
Identity = 295/305 (96.72%), Postives = 301/305 (98.69%), Query Frame = 0


Query: 1   MSSANPYFDDVRTKPEVIDPPQDDDMMDVSESVSDPARNGGKPNVAVSSSVRDLLECPVC 60
           M+SANPYFDD+R KPEVIDPPQDDDMMDVSESVSDPAR GGKPNV VSSSVR+LLECPVC
Sbjct: 1   MTSANPYFDDIRNKPEVIDPPQDDDMMDVSESVSDPARAGGKPNV-VSSSVRELLECPVC 60

Query: 61  LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCKYQTF 120
           LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCKYQTF
Sbjct: 61  LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCKYQTF 120

Query: 121 GCVGIYPYYSKLKHESQCVYRPYNCPYAGSECTVVGDIPFLVAHLKDDHKVDMHNGSTFN 180
           GCVGIYPYYSKLKHESQC+YRPYNCPYAGSEC+V+GDIPFLVAHLKDDHKVDMHNGSTFN
Sbjct: 121 GCVGIYPYYSKLKHESQCIYRPYNCPYAGSECSVIGDIPFLVAHLKDDHKVDMHNGSTFN 180

Query: 181 HRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLRFMGDDNEAKNYS 240
           HRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLRFMGDDNEAKNYS
Sbjct: 181 HRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLRFMGDDNEAKNYS 240

Query: 241 YSLEVGGNGRKTVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR 300
           YSLEVGGNGRK VWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR
Sbjct: 241 YSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR 300

Query: 301 IWKEQ 306
           IWKEQ
Sbjct: 301 IWKEQ 304

[Close]

BLAST of Carg01986-RA vs. TrEMBL
Match: tr|A0A0A0KRN7|A0A0A0KRN7_CUCSA (E3 ubiquitin-protein ligase OS=Cucumis sativus OX=3659 GN=Csa_5G623730 PE=3 SV=1)

HSP 1 Score: 632.9 bits (1631), Expect = 3.8e-178
Identity = 294/305 (96.39%), Postives = 302/305 (99.02%), Query Frame = 0


Query: 1   MSSANPYFDDVRTKPEVIDPPQDDDMMDVSESVSDPARNGGKPNVAVSSSVRDLLECPVC 60
           M+SANPYFDD+R KPEVIDPPQDDDMMDVSESVSDPAR+GGKPNV VSSSVR+LLECPVC
Sbjct: 1   MTSANPYFDDIRNKPEVIDPPQDDDMMDVSESVSDPARSGGKPNV-VSSSVRELLECPVC 60

Query: 61  LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCKYQTF 120
           LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCKYQTF
Sbjct: 61  LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCKYQTF 120

Query: 121 GCVGIYPYYSKLKHESQCVYRPYNCPYAGSECTVVGDIPFLVAHLKDDHKVDMHNGSTFN 180
           GCVGIYPYYSKLKHESQC+YRPYNCPYAGSEC+V+GDIPFLV+HLKDDHKVDMHNGSTFN
Sbjct: 121 GCVGIYPYYSKLKHESQCIYRPYNCPYAGSECSVIGDIPFLVSHLKDDHKVDMHNGSTFN 180

Query: 181 HRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLRFMGDDNEAKNYS 240
           HRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLRFMGDDNEAKNYS
Sbjct: 181 HRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLRFMGDDNEAKNYS 240

Query: 241 YSLEVGGNGRKTVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR 300
           YSLEVGGNGRK VWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR
Sbjct: 241 YSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR 300

Query: 301 IWKEQ 306
           IWKEQ
Sbjct: 301 IWKEQ 304

[Close]

BLAST of Carg01986-RA vs. TrEMBL
Match: tr|A0A067KWI1|A0A067KWI1_JATCU (E3 ubiquitin-protein ligase OS=Jatropha curcas OX=180498 GN=JCGZ_24516 PE=3 SV=1)

HSP 1 Score: 615.9 bits (1587), Expect = 4.8e-173
Identity = 283/305 (92.79%), Postives = 298/305 (97.70%), Query Frame = 0


Query: 1   MSSANPYFDDVRTKPEVIDPPQDDDMMDVSESVSDPARNGGKPNVAVSSSVRDLLECPVC 60
           M+S NP+F+D+R+KPEVIDPPQ++DMMDVSESV+DPA+   KPNV VSSSVR+LLECPVC
Sbjct: 1   MASGNPFFEDMRSKPEVIDPPQNEDMMDVSESVNDPAQMALKPNVTVSSSVRELLECPVC 60

Query: 61  LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCKYQTF 120
           LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCKYQ+F
Sbjct: 61  LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCKYQSF 120

Query: 121 GCVGIYPYYSKLKHESQCVYRPYNCPYAGSECTVVGDIPFLVAHLKDDHKVDMHNGSTFN 180
           GC+GIYPYYSKLKHESQCVYRPYNCPYAGSECTV+GDIP+LVAHLKDDHKVDMHNGSTFN
Sbjct: 121 GCLGIYPYYSKLKHESQCVYRPYNCPYAGSECTVIGDIPYLVAHLKDDHKVDMHNGSTFN 180

Query: 181 HRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLRFMGDDNEAKNYS 240
           HRYVKSNP EVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLRFMGDDNEAKNYS
Sbjct: 181 HRYVKSNPHEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLRFMGDDNEAKNYS 240

Query: 241 YSLEVGGNGRKTVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR 300
           YSLEVGGNGRK +WQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR
Sbjct: 241 YSLEVGGNGRKMIWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR 300

Query: 301 IWKEQ 306
           IWKEQ
Sbjct: 301 IWKEQ 305

[Close]

BLAST of Carg01986-RA vs. TrEMBL
Match: tr|A0A2C9UXB5|A0A2C9UXB5_MANES (E3 ubiquitin-protein ligase OS=Manihot esculenta OX=3983 GN=MANES_11G004100 PE=3 SV=1)

HSP 1 Score: 613.6 bits (1581), Expect = 2.4e-172
Identity = 282/305 (92.46%), Postives = 296/305 (97.05%), Query Frame = 0


Query: 1   MSSANPYFDDVRTKPEVIDPPQDDDMMDVSESVSDPARNGGKPNVAVSSSVRDLLECPVC 60
           M+S NP FDD+R+KPEVIDPPQ++DMMDV ESV+DPA+   KPNV VSSSVR+LLECPVC
Sbjct: 1   MASGNPLFDDMRSKPEVIDPPQNEDMMDVGESVNDPAQTALKPNVTVSSSVRELLECPVC 60

Query: 61  LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCKYQTF 120
           LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCKYQ+F
Sbjct: 61  LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCKYQSF 120

Query: 121 GCVGIYPYYSKLKHESQCVYRPYNCPYAGSECTVVGDIPFLVAHLKDDHKVDMHNGSTFN 180
           GC+GIYPYYSKLKHESQCV+RPYNCPYAGSECTV+GDIP+LVAHLKDDHKVDMHNGSTFN
Sbjct: 121 GCMGIYPYYSKLKHESQCVFRPYNCPYAGSECTVIGDIPYLVAHLKDDHKVDMHNGSTFN 180

Query: 181 HRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLRFMGDDNEAKNYS 240
           HRYVKSNP EVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLRFMGDDNEAKNYS
Sbjct: 181 HRYVKSNPHEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLRFMGDDNEAKNYS 240

Query: 241 YSLEVGGNGRKTVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR 300
           YSLEVGGNGRK +WQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR
Sbjct: 241 YSLEVGGNGRKMIWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR 300

Query: 301 IWKEQ 306
           IWKEQ
Sbjct: 301 IWKEQ 305

The following BLAST results are available for this feature:

BLAST of Carg01986-RA vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023524871.1	3.5e-183	99.67	E3 ubiquitin-protein ligase DIS1 [Cucurbita pepo subsp. pepo] >XP_023524879.1 E3...	[more]
XP_022956691.1	2.3e-182	99.34	E3 ubiquitin-protein ligase DIS1 [Cucurbita moschata] >XP_022956692.1 E3 ubiquit...	[more]
XP_022992030.1	6.6e-182	99.02	E3 ubiquitin-protein ligase DIS1 [Cucurbita maxima]	[more]
XP_022139330.1	2.3e-179	97.05	E3 ubiquitin-protein ligase SINAT5-like [Momordica charantia]	[more]
XP_023541838.1	2.3e-179	97.38	E3 ubiquitin-protein ligase DIS1-like isoform X2 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Carg01986-RA vs. TAIR10


Match Name	E-value	Identity	Description
AT3G61790.1	1.1e-135	83.98	Protein with RING/U-box and TRAF-like domains	[more]
AT4G27880.1	6.2e-134	81.51	Protein with RING/U-box and TRAF-like domains	[more]
AT3G58040.1	8.4e-131	78.87	seven in absentia of Arabidopsis 2	[more]
AT2G41980.1	1.1e-127	75.09	Protein with RING/U-box and TRAF-like domains	[more]
AT5G53360.1	2.8e-102	81.37	TRAF-like superfamily protein	[more]

BLAST of Carg01986-RA vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q10L91\|DIS1_ORYSJ	3.8e-157	86.96	E3 ubiquitin-protein ligase DIS1 OS=Oryza sativa subsp. japonica OX=39947 GN=DIS...	[more]
sp\|Q84JL3\|SINA3_ARATH	2.0e-134	83.98	E3 ubiquitin-protein ligase SINAT3 OS=Arabidopsis thaliana OX=3702 GN=SINAT3 PE=...	[more]
sp\|Q9STN8\|SINA4_ARATH	1.1e-132	81.51	E3 ubiquitin-protein ligase SINAT4 OS=Arabidopsis thaliana OX=3702 GN=SINAT4 PE=...	[more]
sp\|Q8S3N1\|SINA5_ARATH	1.9e-132	75.78	E3 ubiquitin-protein ligase SINAT5 OS=Arabidopsis thaliana OX=3702 GN=SINAT5 PE=...	[more]
sp\|Q9M2P4\|SINA2_ARATH	1.5e-129	78.87	E3 ubiquitin-protein ligase SINAT2 OS=Arabidopsis thaliana OX=3702 GN=SINAT2 PE=...	[more]

BLAST of Carg01986-RA vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A1S3BGX1\|A0A1S3BGX1_CUCME	3.8e-178	96.72	E3 ubiquitin-protein ligase OS=Cucumis melo OX=3656 GN=LOC103489502 PE=3 SV=1	[more]
tr\|E5GCK0\|E5GCK0_CUCME	3.8e-178	96.72	E3 ubiquitin-protein ligase OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1	[more]
tr\|A0A0A0KRN7\|A0A0A0KRN7_CUCSA	3.8e-178	96.39	E3 ubiquitin-protein ligase OS=Cucumis sativus OX=3659 GN=Csa_5G623730 PE=3 SV=1	[more]
tr\|A0A067KWI1\|A0A067KWI1_JATCU	4.8e-173	92.79	E3 ubiquitin-protein ligase OS=Jatropha curcas OX=180498 GN=JCGZ_24516 PE=3 SV=1	[more]
tr\|A0A2C9UXB5\|A0A2C9UXB5_MANES	2.4e-172	92.46	E3 ubiquitin-protein ligase OS=Manihot esculenta OX=3983 GN=MANES_11G004100 PE=3...	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **Molecular Function**
Term	Definition
GO:0008270	zinc ion binding
GO:0005515	protein binding

Vocabulary: **Biological Process**
Term	Definition
GO:0006511	ubiquitin-dependent protein catabolic process
GO:0007275	multicellular organism development

Vocabulary: **Cellular Component**
Term	Definition
GO:0005634	nucleus

Vocabulary: **INTERPRO**
Term	Definition
IPR001841	Znf_RING
IPR013010	Znf_SIAH
IPR004162	SINA-like
IPR018121	7-in-absentia-prot_TRAF-dom
IPR008974	TRAF-like
IPR013083	Znf_RING/FYVE/PHD

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0007275	multicellular organism development
biological_process	GO:0006511	ubiquitin-dependent protein catabolic process
cellular_component	GO:0005634	nucleus
molecular_function	GO:0005515	protein binding
molecular_function	GO:0008270	zinc ion binding

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Carg01986	Carg01986	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Carg01986-RA	Carg01986-RA-protein	polypeptide

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg01986-RA.exon.1	Carg01986-RA.exon.1	exon
Carg01986-RA.exon.2	Carg01986-RA.exon.2	exon
Carg01986-RA.exon.3	Carg01986-RA.exon.3	exon

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg01986-RA.five_prime_UTR.1	Carg01986-RA.five_prime_UTR.1	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg01986-RA.CDS.1	Carg01986-RA.CDS.1	CDS
Carg01986-RA.CDS.2	Carg01986-RA.CDS.2	CDS
Carg01986-RA.CDS.3	Carg01986-RA.CDS.3	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg01986-RA.three_prime_UTR.1	Carg01986-RA.three_prime_UTR.1	three_prime_UTR

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	G3DSA:3.30.40.10		coord: 111..165 e-value: 8.2E-16 score: 60.1
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	G3DSA:3.30.40.10		coord: 21..110 e-value: 5.6E-9 score: 37.6
IPR008974	TRAF-like	GENE3D	G3DSA:2.60.210.10		coord: 167..299 e-value: 1.6E-33 score: 117.2
IPR008974	TRAF-like	SUPERFAMILY	SSF49599	TRAF domain-like	coord: 110..302
IPR018121	Seven-in-absentia protein, TRAF-like domain	PFAM	PF03145	Sina	coord: 99..298 e-value: 1.3E-74 score: 250.4
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..45
None	No IPR available	PANTHER	PTHR10315:SF29	SUBFAMILY NOT NAMED	coord: 6..305
None	No IPR available	CDD	cd03829	Sina	coord: 171..299 e-value: 1.61867E-62 score: 195.683
None	No IPR available	CDD	cd16571	RING-HC_SIAHs	coord: 55..93 e-value: 8.34797E-15 score: 66.1542
None	No IPR available	SUPERFAMILY	SSF57850	RING/U-box	coord: 49..97
IPR004162	E3 ubiquitin-protein ligase SIN-like	PANTHER	PTHR10315	E3 UBIQUITIN PROTEIN LIGASE SIAH	coord: 6..305
IPR013010	Zinc finger, SIAH-type	PROSITE	PS51081	ZF_SIAH	coord: 110..170 score: 13.827
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 57..93 score: 9.347