Carg00784-RA (mRNA) Silver-seed gourd

NameCarg00784-RA
TypemRNA
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionN-acetylglutamate synthase
LocationCucurbita_argyrosperma_scaffold_014 : 1438739 .. 1439391 (+)
Sequence length456
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTACTCCCACATCGAGGACGTGGGTTTTTCCGAAGAGTAATTACTGGAAAAATTGGTCTGATTTTAAGGCGGTGGGTGTGGAGCGTGGGTGCAGAGAGAATTATTTGCGGCGGACAGTTCAGGATCCGACATGCCATGAGAGTGGGAGAAGAAAATGGAACTGGGGATTGGCGGCGGTGAGAGGCGGCAAACGGGGGGAGGCTAAAAATGATGCGTATGATAAAATTCCTGAAGAAGATAGGAGGTTTATTGAAGAATTCAGAGATGCTTATCCCTATATATTCTCCCATATAGGCCGTACTTTTGTCGTCGTTATATCTGCCGAAATTGTGGCTAGTCCTCGCCTCCATGCCTTGCTCAAGGTCTCTCTTCCTCCTTTCCTGAAAAAAAAAGAAGTTACTTTCAATTCTCTGTCAATGGCGGATGATTATGCACTGTAGTCTTTGTCTTAGTTTTAGTTGCTTGGGGACTTAACCGAGATAGAGAAATCATCGAGTAGACACCCTTGTTTACATCGCCACAAAAAAAGTTTCTTTTTGTTTAATTATTTTAAACTCAGAAAGTAAAAGCTTTGGAGTTCGTTACCTTTTATGTTTTTGGTTTCTTGAAAATCCGATTTTGGAAGTATTGTGAAGTGGAAGCGCTTTGA

mRNA sequence

ATGGCTACTCCCACATCGAGGACGTGGGTTTTTCCGAAGAGTAATTACTGGAAAAATTGGTCTGATTTTAAGGCGGTGGGTGTGGAGCGTGGGTGCAGAGAGAATTATTTGCGGCGGACAGTTCAGGATCCGACATGCCATGAGAGTGGGAGAAGAAAATGGAACTGGGGATTGGCGGCGGTGAGAGGCGGCAAACGGGGGGAGGCTAAAAATGATGCGTATGATAAAATTCCTGAAGAAGATAGGAGGTTTATTGAAGAATTCAGAGATGCTTATCCCTATATATTCTCCCATATAGGCCGTACTTTTGTCGTCGTTATATCTGCCGAAATTGTGGCTAGTCCTCGCCTCCATGCCTTGCTCAAGAAAGTAAAAGCTTTGGAGTTCGTTACCTTTTATGTTTTTGGTTTCTTGAAAATCCGATTTTGGAAGTATTGTGAAGTGGAAGCGCTTTGA

Coding sequence (CDS)

ATGGCTACTCCCACATCGAGGACGTGGGTTTTTCCGAAGAGTAATTACTGGAAAAATTGGTCTGATTTTAAGGCGGTGGGTGTGGAGCGTGGGTGCAGAGAGAATTATTTGCGGCGGACAGTTCAGGATCCGACATGCCATGAGAGTGGGAGAAGAAAATGGAACTGGGGATTGGCGGCGGTGAGAGGCGGCAAACGGGGGGAGGCTAAAAATGATGCGTATGATAAAATTCCTGAAGAAGATAGGAGGTTTATTGAAGAATTCAGAGATGCTTATCCCTATATATTCTCCCATATAGGCCGTACTTTTGTCGTCGTTATATCTGCCGAAATTGTGGCTAGTCCTCGCCTCCATGCCTTGCTCAAGAAAGTAAAAGCTTTGGAGTTCGTTACCTTTTATGTTTTTGGTTTCTTGAAAATCCGATTTTGGAAGTATTGTGAAGTGGAAGCGCTTTGA

Protein sequence

MATPTSRTWVFPKSNYWKNWSDFKAVGVERGCRENYLRRTVQDPTCHESGRRKWNWGLAAVRGGKRGEAKNDAYDKIPEEDRRFIEEFRDAYPYIFSHIGRTFVVVISAEIVASPRLHALLKKVKALEFVTFYVFGFLKIRFWKYCEVEAL
BLAST of Carg00784-RA vs. NCBI nr
Match: XP_022950017.1 (probable amino-acid acetyltransferase NAGS2, chloroplastic isoform X2 [Cucurbita moschata])

HSP 1 Score: 260.8 bits (665), Expect = 2.9e-66
Identity = 123/127 (96.85%), Postives = 124/127 (97.64%), Query Frame = 0

Query: 1   MATPTSRTWVFPKSNYWKNWSDFKAVGVERGCRENYLRRTVQDPTCHESGRRKWNWGLAA 60
           MATPTSRTWVFPKSNYWKNWSDFKAVGVERGCRENYLRRTVQDPTCHESGRRKWNWGLAA
Sbjct: 1   MATPTSRTWVFPKSNYWKNWSDFKAVGVERGCRENYLRRTVQDPTCHESGRRKWNWGLAA 60

Query: 61  VRGGKRGEAKNDAYDKIPEEDRRFIEEFRDAYPYIFSHIGRTFVVVISAEIVASPRLHAL 120
           VRGGKRGEAKNDAYDKIPEEDRRFIEEFRDAYPYIFSHIGRTFVVVISAEIVASPRLHAL
Sbjct: 61  VRGGKRGEAKNDAYDKIPEEDRRFIEEFRDAYPYIFSHIGRTFVVVISAEIVASPRLHAL 120

Query: 121 LKKVKAL 128
           LK +  L
Sbjct: 121 LKDIAFL 127

BLAST of Carg00784-RA vs. NCBI nr
Match: XP_023543136.1 (probable amino-acid acetyltransferase NAGS2, chloroplastic isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 260.4 bits (664), Expect = 3.8e-66
Identity = 122/127 (96.06%), Postives = 124/127 (97.64%), Query Frame = 0

Query: 1   MATPTSRTWVFPKSNYWKNWSDFKAVGVERGCRENYLRRTVQDPTCHESGRRKWNWGLAA 60
           MATPTSRTWVFPKSNYWKNWSDFKAVGVERGCRENYLRRTVQDPTCHESGRRKWNWGLAA
Sbjct: 1   MATPTSRTWVFPKSNYWKNWSDFKAVGVERGCRENYLRRTVQDPTCHESGRRKWNWGLAA 60

Query: 61  VRGGKRGEAKNDAYDKIPEEDRRFIEEFRDAYPYIFSHIGRTFVVVISAEIVASPRLHAL 120
           VRGGKRGEAKNDAYDK+PEEDRRFIEEFRDAYPYIFSHIGRTFVVVISAEIVASPRLHAL
Sbjct: 61  VRGGKRGEAKNDAYDKVPEEDRRFIEEFRDAYPYIFSHIGRTFVVVISAEIVASPRLHAL 120

Query: 121 LKKVKAL 128
           LK +  L
Sbjct: 121 LKDIAFL 127

BLAST of Carg00784-RA vs. NCBI nr
Match: XP_022977436.1 (probable amino-acid acetyltransferase NAGS2, chloroplastic isoform X2 [Cucurbita maxima])

HSP 1 Score: 250.4 bits (638), Expect = 4.0e-63
Identity = 117/127 (92.13%), Postives = 122/127 (96.06%), Query Frame = 0

Query: 1   MATPTSRTWVFPKSNYWKNWSDFKAVGVERGCRENYLRRTVQDPTCHESGRRKWNWGLAA 60
           MATPTSRTWVFP SN+WK WSDFKAVGVERGCRENYLRRTVQDPTCHE+GRRKWNWGLAA
Sbjct: 1   MATPTSRTWVFPNSNHWKIWSDFKAVGVERGCRENYLRRTVQDPTCHENGRRKWNWGLAA 60

Query: 61  VRGGKRGEAKNDAYDKIPEEDRRFIEEFRDAYPYIFSHIGRTFVVVISAEIVASPRLHAL 120
           VRGGKRGEAKNDAYDK+PEEDRRFIEEFRDAYPYIFSHIGRTFVVVISAEIVASPRLHAL
Sbjct: 61  VRGGKRGEAKNDAYDKVPEEDRRFIEEFRDAYPYIFSHIGRTFVVVISAEIVASPRLHAL 120

Query: 121 LKKVKAL 128
           L+ +  L
Sbjct: 121 LEDIAFL 127

BLAST of Carg00784-RA vs. NCBI nr
Match: AFA37979.1 (N-acetylglutamate synthase [Cucurbita maxima x Cucurbita moschata])

HSP 1 Score: 249.2 bits (635), Expect = 8.8e-63
Identity = 116/127 (91.34%), Postives = 122/127 (96.06%), Query Frame = 0

Query: 1   MATPTSRTWVFPKSNYWKNWSDFKAVGVERGCRENYLRRTVQDPTCHESGRRKWNWGLAA 60
           MATPT+RTWVFP SN+WK WSDFKAVGVERGCRENYLRRTVQDPTCHE+GRRKWNWGLAA
Sbjct: 1   MATPTTRTWVFPNSNHWKIWSDFKAVGVERGCRENYLRRTVQDPTCHENGRRKWNWGLAA 60

Query: 61  VRGGKRGEAKNDAYDKIPEEDRRFIEEFRDAYPYIFSHIGRTFVVVISAEIVASPRLHAL 120
           VRGGKRGEAKNDAYDK+PEEDRRFIEEFRDAYPYIFSHIGRTFVVVISAEIVASPRLHAL
Sbjct: 61  VRGGKRGEAKNDAYDKVPEEDRRFIEEFRDAYPYIFSHIGRTFVVVISAEIVASPRLHAL 120

Query: 121 LKKVKAL 128
           L+ +  L
Sbjct: 121 LEDIAFL 127

BLAST of Carg00784-RA vs. NCBI nr
Match: XP_008440407.1 (PREDICTED: probable amino-acid acetyltransferase NAGS2, chloroplastic isoform X1 [Cucumis melo])

HSP 1 Score: 163.3 bits (412), Expect = 6.4e-37
Identity = 91/140 (65.00%), Postives = 103/140 (73.57%), Query Frame = 0

Query: 1   MATPTSRTWVFPKSNYWKN-WSDFKAVGVERGCRENYLRRTVQDPTCHESGRRKWNWGLA 60
           MATPT+RTWVFPKSN+ ++ W DF +VGV RGCR+N  R T++ P   ESGRRK N GLA
Sbjct: 1   MATPTTRTWVFPKSNHREDTWFDFMSVGVGRGCRKNLRRWTIKGPMRCESGRRKLNTGLA 60

Query: 61  A-----------VRGGKRGEAKNDA-YDKIPEEDRRFIEEFRDAYPYIFSHIGRTFVVVI 120
           A              G   E KNDA Y+K+ EEDRRFIEEFR+AYPYIFSHIGRTFVVVI
Sbjct: 61  AGKVCNREEVKTTAEGPEIETKNDAYYNKVTEEDRRFIEEFREAYPYIFSHIGRTFVVVI 120

Query: 121 SAEIVASPRLHALLKKVKAL 128
           SAEIVASPRLHALLK +  L
Sbjct: 121 SAEIVASPRLHALLKDIAFL 140

BLAST of Carg00784-RA vs. TAIR10
Match: AT4G37670.2 (N-acetyl-l-glutamate synthase 2)

HSP 1 Score: 52.8 bits (125), Expect = 2.2e-07
Identity = 25/59 (42.37%), Postives = 39/59 (66.10%), Query Frame = 0

Query: 69  AKNDAYDKIPEEDRRFIEEFRDAYPYIFSHIGRTFVVVISAEIVASPRLHALLKKVKAL 128
           A  D   + P +D++F+  FR+A+PY+++H G TFVV+IS EI+A     A+LK +  L
Sbjct: 69  AAGDVEAEHPVDDKQFVRWFREAWPYLWAHRGCTFVVIISGEIIAGSSCDAILKDIAFL 127

BLAST of Carg00784-RA vs. TAIR10
Match: AT2G22910.1 (N-acetyl-l-glutamate synthase 1)

HSP 1 Score: 41.6 bits (96), Expect = 5.1e-04
Identity = 18/47 (38.30%), Postives = 31/47 (65.96%), Query Frame = 0

Query: 81  DRRFIEEFRDAYPYIFSHIGRTFVVVISAEIVASPRLHALLKKVKAL 128
           D++F+  FR+A+PY+++H   TFVV IS +++  P    +LK +  L
Sbjct: 74  DKQFVRWFREAWPYLWAHRSCTFVVTISGDVLDGPYCDLVLKDIAFL 120

BLAST of Carg00784-RA vs. Swiss-Prot
Match: sp|B5X4Z4|NAGS2_ARATH (Probable amino-acid acetyltransferase NAGS2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NAGS2 PE=1 SV=1)

HSP 1 Score: 52.8 bits (125), Expect = 4.0e-06
Identity = 25/59 (42.37%), Postives = 39/59 (66.10%), Query Frame = 0

Query: 69  AKNDAYDKIPEEDRRFIEEFRDAYPYIFSHIGRTFVVVISAEIVASPRLHALLKKVKAL 128
           A  D   + P +D++F+  FR+A+PY+++H G TFVV+IS EI+A     A+LK +  L
Sbjct: 69  AAGDVEAEHPVDDKQFVRWFREAWPYLWAHRGCTFVVIISGEIIAGSSCDAILKDIAFL 127

BLAST of Carg00784-RA vs. TrEMBL
Match: tr|H6V8U2|H6V8U2_9ROSI (N-acetylglutamate synthase OS=Cucurbita maxima x Cucurbita moschata OX=334420 GN=NAGS PE=2 SV=1)

HSP 1 Score: 249.2 bits (635), Expect = 5.8e-63
Identity = 116/127 (91.34%), Postives = 122/127 (96.06%), Query Frame = 0

Query: 1   MATPTSRTWVFPKSNYWKNWSDFKAVGVERGCRENYLRRTVQDPTCHESGRRKWNWGLAA 60
           MATPT+RTWVFP SN+WK WSDFKAVGVERGCRENYLRRTVQDPTCHE+GRRKWNWGLAA
Sbjct: 1   MATPTTRTWVFPNSNHWKIWSDFKAVGVERGCRENYLRRTVQDPTCHENGRRKWNWGLAA 60

Query: 61  VRGGKRGEAKNDAYDKIPEEDRRFIEEFRDAYPYIFSHIGRTFVVVISAEIVASPRLHAL 120
           VRGGKRGEAKNDAYDK+PEEDRRFIEEFRDAYPYIFSHIGRTFVVVISAEIVASPRLHAL
Sbjct: 61  VRGGKRGEAKNDAYDKVPEEDRRFIEEFRDAYPYIFSHIGRTFVVVISAEIVASPRLHAL 120

Query: 121 LKKVKAL 128
           L+ +  L
Sbjct: 121 LEDIAFL 127

BLAST of Carg00784-RA vs. TrEMBL
Match: tr|A0A1S3B1T5|A0A1S3B1T5_CUCME (probable amino-acid acetyltransferase NAGS2, chloroplastic isoform X1 OS=Cucumis melo OX=3656 GN=LOC103484863 PE=3 SV=1)

HSP 1 Score: 163.3 bits (412), Expect = 4.2e-37
Identity = 91/140 (65.00%), Postives = 103/140 (73.57%), Query Frame = 0

Query: 1   MATPTSRTWVFPKSNYWKN-WSDFKAVGVERGCRENYLRRTVQDPTCHESGRRKWNWGLA 60
           MATPT+RTWVFPKSN+ ++ W DF +VGV RGCR+N  R T++ P   ESGRRK N GLA
Sbjct: 1   MATPTTRTWVFPKSNHREDTWFDFMSVGVGRGCRKNLRRWTIKGPMRCESGRRKLNTGLA 60

Query: 61  A-----------VRGGKRGEAKNDA-YDKIPEEDRRFIEEFRDAYPYIFSHIGRTFVVVI 120
           A              G   E KNDA Y+K+ EEDRRFIEEFR+AYPYIFSHIGRTFVVVI
Sbjct: 61  AGKVCNREEVKTTAEGPEIETKNDAYYNKVTEEDRRFIEEFREAYPYIFSHIGRTFVVVI 120

Query: 121 SAEIVASPRLHALLKKVKAL 128
           SAEIVASPRLHALLK +  L
Sbjct: 121 SAEIVASPRLHALLKDIAFL 140

BLAST of Carg00784-RA vs. TrEMBL
Match: tr|A0A0A0KFZ3|A0A0A0KFZ3_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G495740 PE=4 SV=1)

HSP 1 Score: 151.8 bits (382), Expect = 1.3e-33
Identity = 87/136 (63.97%), Postives = 99/136 (72.79%), Query Frame = 0

Query: 1   MATPTSRTWVFPKSNYWK-NWSDFKAVGVERGCRENYLR-RTVQDPTCHESGRRKWNWGL 60
           MATPT+RTWVFPKS+Y +    DF++VGV RG R+N  R  T++ P C ESG+RK N GL
Sbjct: 1   MATPTTRTWVFPKSSYREITCFDFESVGVGRGYRKNLRRWTTIKGPMCCESGKRKLNMGL 60

Query: 61  AA-----------VRGGKRGEAKNDA-YDKIPEEDRRFIEEFRDAYPYIFSHIGRTFVVV 120
           +A              G   E KNDA Y K+ EEDRRFIEEFR+AYPYIFSHIGRTFVVV
Sbjct: 61  SAGKVCNREEVKTTAEGPEIETKNDAYYTKVTEEDRRFIEEFREAYPYIFSHIGRTFVVV 120

Query: 121 ISAEIVASPRLHALLK 123
           ISAEIVASPRLHALLK
Sbjct: 121 ISAEIVASPRLHALLK 136

BLAST of Carg00784-RA vs. TrEMBL
Match: tr|A0A2P5FSE8|A0A2P5FSE8_9ROSA (Uncharacterized protein OS=Trema orientalis OX=63057 GN=TorRG33x02_033310 PE=4 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 1.7e-09
Identity = 40/95 (42.11%), Postives = 54/95 (56.84%), Query Frame = 0

Query: 28  VERGCRENYLRRTVQDPTCHESGRRKWNWGLAAVRGGKRGEAKNDAYDKIPEEDRRFIEE 87
           ++RG +       V    C   GRR+W            G+  N    ++ EED+RF+E 
Sbjct: 9   LQRGKKRKGSSAPVIGRWCCGGGRRRW-----------VGKVSNGVNGELEEEDKRFVEA 68

Query: 88  FRDAYPYIFSHIGRTFVVVISAEIVASPRLHALLK 123
            R+A PYIF+H+GRTFVVV+SAEIVA P L  +LK
Sbjct: 69  LREAQPYIFAHMGRTFVVVLSAEIVAGPCLETILK 92

BLAST of Carg00784-RA vs. TrEMBL
Match: tr|W9RJH1|W9RJH1_9ROSA (Uncharacterized protein OS=Morus notabilis OX=981085 GN=L484_009378 PE=4 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 1.8e-08
Identity = 38/74 (51.35%), Postives = 49/74 (66.22%), Query Frame = 0

Query: 49  SGRRKWNWGLAAVRGGKRGEAKNDAYDKIPEEDRRFIEEFRDAYPYIFSHIGRTFVVVIS 108
           S +RK   G+    G +R     +A   + EEDR F+E  R+A PYIF+HIGRTFVVV+S
Sbjct: 49  SKKRKGPSGIGG--GDRRWRPVREAESAVSEEDRSFVEALREAQPYIFAHIGRTFVVVLS 108

Query: 109 AEIVASPRLHALLK 123
           AEIVA P L ++LK
Sbjct: 109 AEIVAGPCLDSILK 120

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022950017.12.9e-6696.85probable amino-acid acetyltransferase NAGS2, chloroplastic isoform X2 [Cucurbita... [more]
XP_023543136.13.8e-6696.06probable amino-acid acetyltransferase NAGS2, chloroplastic isoform X2 [Cucurbita... [more]
XP_022977436.14.0e-6392.13probable amino-acid acetyltransferase NAGS2, chloroplastic isoform X2 [Cucurbita... [more]
AFA37979.18.8e-6391.34N-acetylglutamate synthase [Cucurbita maxima x Cucurbita moschata][more]
XP_008440407.16.4e-3765.00PREDICTED: probable amino-acid acetyltransferase NAGS2, chloroplastic isoform X1... [more]
Match NameE-valueIdentityDescription
AT4G37670.22.2e-0742.37N-acetyl-l-glutamate synthase 2[more]
AT2G22910.15.1e-0438.30N-acetyl-l-glutamate synthase 1[more]
Match NameE-valueIdentityDescription
sp|B5X4Z4|NAGS2_ARATH4.0e-0642.37Probable amino-acid acetyltransferase NAGS2, chloroplastic OS=Arabidopsis thalia... [more]
Match NameE-valueIdentityDescription
tr|H6V8U2|H6V8U2_9ROSI5.8e-6391.34N-acetylglutamate synthase OS=Cucurbita maxima x Cucurbita moschata OX=334420 GN... [more]
tr|A0A1S3B1T5|A0A1S3B1T5_CUCME4.2e-3765.00probable amino-acid acetyltransferase NAGS2, chloroplastic isoform X1 OS=Cucumis... [more]
tr|A0A0A0KFZ3|A0A0A0KFZ3_CUCSA1.3e-3363.97Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G495740 PE=4 SV=1[more]
tr|A0A2P5FSE8|A0A2P5FSE8_9ROSA1.7e-0942.11Uncharacterized protein OS=Trema orientalis OX=63057 GN=TorRG33x02_033310 PE=4 S... [more]
tr|W9RJH1|W9RJH1_9ROSA1.8e-0851.35Uncharacterized protein OS=Morus notabilis OX=981085 GN=L484_009378 PE=4 SV=1[more]
The following terms have been associated with this mRNA:
Vocabulary: Cellular Component
TermDefinition
GO:0005737cytoplasm
Vocabulary: Molecular Function
TermDefinition
GO:0004042acetyl-CoA:L-glutamate N-acetyltransferase activity
Vocabulary: Biological Process
TermDefinition
GO:0006526arginine biosynthetic process
Vocabulary: INTERPRO
TermDefinition
IPR010167NH2A_AcTrfase
IPR036393AceGlu_kinase-like_sf
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006526 arginine biosynthetic process
biological_process GO:0042967 acyl-carrier-protein biosynthetic process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0004042 acetyl-CoA:L-glutamate N-acetyltransferase activity

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Carg00784Carg00784gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg00784-RA.exon.1Carg00784-RA.exon.1exon
Carg00784-RA.exon.2Carg00784-RA.exon.2exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg00784-RA.CDS.1Carg00784-RA.CDS.1CDS
Carg00784-RA.CDS.2Carg00784-RA.CDS.2CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Carg00784-RACarg00784-RA-proteinpolypeptide


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036393Acetylglutamate kinase-like superfamilyGENE3DG3DSA:3.40.1160.10coord: 82..134
e-value: 1.8E-6
score: 29.5
IPR010167Amino-acid N-acetyltransferasePANTHERPTHR30602AMINO-ACID ACETYLTRANSFERASEcoord: 57..128