Carg00648-RA (mRNA) Silver-seed gourd

NameCarg00648-RA
TypemRNA
OrganismCucurbita argyrosperma (Silver-seed gourd)
Descriptionrandom slug protein 5-like
LocationCucurbita_argyrosperma_scaffold_014 : 778159 .. 780523 (-)
Sequence length972
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TAGATTACAGTGTTTTTTATATGTATGATAGCCTTCTGAATGTTGTAGGTTGAATTTGCTCTGTGCGTATTCAACATGTTCCTTTTTAAACGAAGGGGCTCCCAAAACAATGGTGAAATCGATGATTCTTCACAGCAGGATGCGAAGGTAGACAGCTACTTTTAACCTTTTTTTTCCCCTTTCAAATGTAATTTTATAGAGACAGAAGTGATGAATTGAACGCTCTTTTTATGATCTGTAATGAACAGATCAATGAACTAAGAGCAGCACTCGGACATCTATCCGGTCATAGTTTAAAGTACTGTGATGATGCATGCCTTCGGAGATATTTGGTAGCTCGGAATTGGGATGTTCACAAGTCAAAGAAAATGCTGGATGACTCACTCAAATGGAGGGCAACTTATAAGCCCGAGGAAATCAGATGGGTTAGTTTGATATCAATCTTTAAGTTCTTTACCTTTTCTTTAAACTCTCTGAGCTACTTCTAGGTACGAATGTGCGTTTATATAATTATAAGAGGGGGAACGATTGCCTTCTAAACTTTTGATCGAAGTAATTCCAAGTTAGTGAGAACAAACTAGTGTCAAACAGAAAGAGGAAAAAAGCTTATTCCTAACTATTTAGATGCTGAAAATTCTTCAATCTCAATCCCCGTGGAAATTTAGGACTATATATATATATTGACTTCCGATAGTACTCATAAAATTAGAAGGTAGTTTCTTTAAGAAAGGGAACATTTCAGTTTGTTTAGGAAAATGGTCCAAATTTTAGGACTGTATTGACTTTTCTTTTTATTAGTATCGTAAATTTAGATTTGTAGGCTTATTGTAGGATGAAGGGATATAGTTACCTACATTCCAGGCCGAAAGTTGGTCTTGCAATCATTCTCCACCCTCCTATCCATCTCAGACTTTCTCTGCCTTTCCTTCTCATAAATGATCTTATGGCCTTATTCAAAACAGATATCTAAAAATATATACATCAAGTATCGTCAATAGTTCACATGCTATCTTGCCACTTATTTTCTTTCTCTACAGCTCATCTTGAATCAACGTGCTTTTAAGTCAATAGACGTGTTTTTTTTTTTTTTTTGGGCAAGGAAAAATCTAAATCTGCATTATTCCTATCAAATAGAATTTATCTTGAAACATTAATAATAGTAGTAACAATGATATTTTATTATTATTATTATTATTTCCTAATATTCAGTTAAGAGAAAACAAAATGTGATTAATAATTTTGATTATACCTGAACTTCCTATAATTAACTATGTTAATAAGAGCAATCGTGAGTGATAAGCGATAATACACATACATTCACTTTATATATCACAAACTTTCGTTGTGATGCAGCACGAAGTGGCACATGAAGGTGAAACGGGCAAATCTTTTAGAGCAAATTTCTATGATCGTTTTGGTCGAACCGTGCTCATATCCAGGCCAGGGATGCAGGTATTTTATTAAGATTTTCTAGGGTTAGTGCAAAATCAAAATTTAGAACCAGTTTTAACCAAATCAATATGATTAGTATTTGCGTTATAAATTCCCATAATAGCATCGTTGTGAAACAAAGCATGGTTGAAAGAAAAACTATGTTAAATAAGTTTTCAAACTTACATGTTTAATAAATTCAAAAATAAAAGTTGAAAGATTAAACTTTAATTTAAACGAAGAAATAAATAAATAATAATAATAAAAATTGACTTGGTTTCTTGGTGAAATTTCAGAATACAAATTCGCCAGAAGACAATGTCCGGCATGTAGTGTATGTTTTAGAGAACACCATTATGAACCTTCGCGATGGCCAAGAACAGATATCGTGGCTGATAGACTTTACTGGATTTACAATAAACACCAACATCTCTGTCAAAGCAGCACGGGGCATAATTAACATACTGCAGAGCCACTATCCAGAAAGGCTTGCTATTTCCTTTCTCTACAATCCTCCAAGAATCTTTCAAGCCTTCTGGAAGGTTAGAGTTCGAAGCTGTCTTTACTTCATTACTGAATGAATGTTGAACTTTGAAGTCGATTTTGGTTCTAAAATCTCTCCTTTTGGAAGGCTATCGAGTACTTTATTGACTCAAAGACCGCTCAGAAAGTACACTTTCTATACCCCAACAACCAGAACAGTGTGGAGCTAATGAAATCTTTCTTCGACATGGAAAACCTTCCAAGTGTATTTGGGGGGAAAGCCACTCTAACTTACGACCATGAAGAGTTCTCAAAAATGATGGCAATAGACGACCTGAAAACCGCCAAGTTTTGGGAGTTCAGCGACAAGCCTTGCCGCCATATGAACGGGCATTCAGGCCCAGAGGTGGCACCGGAAGAGCCAATGCCCATCTCAGTTTCATCAGCTTAG

mRNA sequence

TAGATTACAGTGTTTTTTATATGTATGATAGCCTTCTGAATGTTGTAGGTTGAATTTGCTCTGTGCGTATTCAACATGTTCCTTTTTAAACGAAGGGGCTCCCAAAACAATGGTGAAATCGATGATTCTTCACAGCAGGATGCGAAGATCAATGAACTAAGAGCAGCACTCGGACATCTATCCGGTCATAGTTTAAAGTACTGTGATGATGCATGCCTTCGGAGATATTTGGTAGCTCGGAATTGGGATGTTCACAAGTCAAAGAAAATGCTGGATGACTCACTCAAATGGAGGGCAACTTATAAGCCCGAGGAAATCAGATGGCACGAAGTGGCACATGAAGGTGAAACGGGCAAATCTTTTAGAGCAAATTTCTATGATCGTTTTGGTCGAACCGTGCTCATATCCAGGCCAGGGATGCAGAATACAAATTCGCCAGAAGACAATGTCCGGCATGTAGTGTATGTTTTAGAGAACACCATTATGAACCTTCGCGATGGCCAAGAACAGATATCGTGGCTGATAGACTTTACTGGATTTACAATAAACACCAACATCTCTGTCAAAGCAGCACGGGGCATAATTAACATACTGCAGAGCCACTATCCAGAAAGGCTTGCTATTTCCTTTCTCTACAATCCTCCAAGAATCTTTCAAGCCTTCTGGAAGGCTATCGAGTACTTTATTGACTCAAAGACCGCTCAGAAAGTACACTTTCTATACCCCAACAACCAGAACAGTGTGGAGCTAATGAAATCTTTCTTCGACATGGAAAACCTTCCAAGTGTATTTGGGGGGAAAGCCACTCTAACTTACGACCATGAAGAGTTCTCAAAAATGATGGCAATAGACGACCTGAAAACCGCCAAGTTTTGGGAGTTCAGCGACAAGCCTTGCCGCCATATGAACGGGCATTCAGGCCCAGAGGTGGCACCGGAAGAGCCAATGCCCATCTCAGTTTCATCAGCTTAG

Coding sequence (CDS)

ATGTTCCTTTTTAAACGAAGGGGCTCCCAAAACAATGGTGAAATCGATGATTCTTCACAGCAGGATGCGAAGATCAATGAACTAAGAGCAGCACTCGGACATCTATCCGGTCATAGTTTAAAGTACTGTGATGATGCATGCCTTCGGAGATATTTGGTAGCTCGGAATTGGGATGTTCACAAGTCAAAGAAAATGCTGGATGACTCACTCAAATGGAGGGCAACTTATAAGCCCGAGGAAATCAGATGGCACGAAGTGGCACATGAAGGTGAAACGGGCAAATCTTTTAGAGCAAATTTCTATGATCGTTTTGGTCGAACCGTGCTCATATCCAGGCCAGGGATGCAGAATACAAATTCGCCAGAAGACAATGTCCGGCATGTAGTGTATGTTTTAGAGAACACCATTATGAACCTTCGCGATGGCCAAGAACAGATATCGTGGCTGATAGACTTTACTGGATTTACAATAAACACCAACATCTCTGTCAAAGCAGCACGGGGCATAATTAACATACTGCAGAGCCACTATCCAGAAAGGCTTGCTATTTCCTTTCTCTACAATCCTCCAAGAATCTTTCAAGCCTTCTGGAAGGCTATCGAGTACTTTATTGACTCAAAGACCGCTCAGAAAGTACACTTTCTATACCCCAACAACCAGAACAGTGTGGAGCTAATGAAATCTTTCTTCGACATGGAAAACCTTCCAAGTGTATTTGGGGGGAAAGCCACTCTAACTTACGACCATGAAGAGTTCTCAAAAATGATGGCAATAGACGACCTGAAAACCGCCAAGTTTTGGGAGTTCAGCGACAAGCCTTGCCGCCATATGAACGGGCATTCAGGCCCAGAGGTGGCACCGGAAGAGCCAATGCCCATCTCAGTTTCATCAGCTTAG

Protein sequence

MFLFKRRGSQNNGEIDDSSQQDAKINELRAALGHLSGHSLKYCDDACLRRYLVARNWDVHKSKKMLDDSLKWRATYKPEEIRWHEVAHEGETGKSFRANFYDRFGRTVLISRPGMQNTNSPEDNVRHVVYVLENTIMNLRDGQEQISWLIDFTGFTINTNISVKAARGIINILQSHYPERLAISFLYNPPRIFQAFWKAIEYFIDSKTAQKVHFLYPNNQNSVELMKSFFDMENLPSVFGGKATLTYDHEEFSKMMAIDDLKTAKFWEFSDKPCRHMNGHSGPEVAPEEPMPISVSSA
BLAST of Carg00648-RA vs. NCBI nr
Match: XP_023543859.1 (phosphatidylinositol transfer protein 3-like [Cucurbita pepo subsp. pepo] >XP_023543860.1 phosphatidylinositol transfer protein 3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 617.8 bits (1592), Expect = 1.9e-173
Identity = 297/298 (99.66%), Postives = 297/298 (99.66%), Query Frame = 0

Query: 1   MFLFKRRGSQNNGEIDDSSQQDAKINELRAALGHLSGHSLKYCDDACLRRYLVARNWDVH 60
           MFLFKRRGSQNNGEIDDSSQQDAKINELR ALGHLSGHSLKYCDDACLRRYLVARNWDVH
Sbjct: 1   MFLFKRRGSQNNGEIDDSSQQDAKINELRTALGHLSGHSLKYCDDACLRRYLVARNWDVH 60

Query: 61  KSKKMLDDSLKWRATYKPEEIRWHEVAHEGETGKSFRANFYDRFGRTVLISRPGMQNTNS 120
           KSKKMLDDSLKWRATYKPEEIRWHEVAHEGETGKSFRANFYDRFGRTVLISRPGMQNTNS
Sbjct: 61  KSKKMLDDSLKWRATYKPEEIRWHEVAHEGETGKSFRANFYDRFGRTVLISRPGMQNTNS 120

Query: 121 PEDNVRHVVYVLENTIMNLRDGQEQISWLIDFTGFTINTNISVKAARGIINILQSHYPER 180
           PEDNVRHVVYVLENTIMNLRDGQEQISWLIDFTGFTINTNISVKAARGIINILQSHYPER
Sbjct: 121 PEDNVRHVVYVLENTIMNLRDGQEQISWLIDFTGFTINTNISVKAARGIINILQSHYPER 180

Query: 181 LAISFLYNPPRIFQAFWKAIEYFIDSKTAQKVHFLYPNNQNSVELMKSFFDMENLPSVFG 240
           LAISFLYNPPRIFQAFWKAIEYFIDSKTAQKVHFLYPNNQNSVELMKSFFDMENLPSVFG
Sbjct: 181 LAISFLYNPPRIFQAFWKAIEYFIDSKTAQKVHFLYPNNQNSVELMKSFFDMENLPSVFG 240

Query: 241 GKATLTYDHEEFSKMMAIDDLKTAKFWEFSDKPCRHMNGHSGPEVAPEEPMPISVSSA 299
           GKATLTYDHEEFSKMMAIDDLKTAKFWEFSDKPCRHMNGHSGPEVAPEEPMPISVSSA
Sbjct: 241 GKATLTYDHEEFSKMMAIDDLKTAKFWEFSDKPCRHMNGHSGPEVAPEEPMPISVSSA 298

BLAST of Carg00648-RA vs. NCBI nr
Match: XP_022950390.1 (CRAL-TRIO domain-containing protein C23B6.04c-like [Cucurbita moschata] >XP_022950391.1 CRAL-TRIO domain-containing protein C23B6.04c-like [Cucurbita moschata])

HSP 1 Score: 611.3 bits (1575), Expect = 1.7e-171
Identity = 294/298 (98.66%), Postives = 295/298 (98.99%), Query Frame = 0

Query: 1   MFLFKRRGSQNNGEIDDSSQQDAKINELRAALGHLSGHSLKYCDDACLRRYLVARNWDVH 60
           MFLFKRRGS NNGE DDSSQQDAKINELRAALGHLSGHSLKYCDDACLRRYLVARNWDVH
Sbjct: 1   MFLFKRRGSLNNGETDDSSQQDAKINELRAALGHLSGHSLKYCDDACLRRYLVARNWDVH 60

Query: 61  KSKKMLDDSLKWRATYKPEEIRWHEVAHEGETGKSFRANFYDRFGRTVLISRPGMQNTNS 120
           KSKKMLDDSLKWRA YKPEEIRWHEVAHEGETGKSFRANFYDRFGR+VLISRPGMQNTNS
Sbjct: 61  KSKKMLDDSLKWRAAYKPEEIRWHEVAHEGETGKSFRANFYDRFGRSVLISRPGMQNTNS 120

Query: 121 PEDNVRHVVYVLENTIMNLRDGQEQISWLIDFTGFTINTNISVKAARGIINILQSHYPER 180
           PEDNVRHVVYVLENTIMNLRDGQEQISWLIDFTGFTINTNISVKAARGIINILQSHYPER
Sbjct: 121 PEDNVRHVVYVLENTIMNLRDGQEQISWLIDFTGFTINTNISVKAARGIINILQSHYPER 180

Query: 181 LAISFLYNPPRIFQAFWKAIEYFIDSKTAQKVHFLYPNNQNSVELMKSFFDMENLPSVFG 240
           LAISFLYNPPRIFQAFWKAIEYFIDSKTAQKVHFLYPNNQNSVELMKSFFDMENLPSVFG
Sbjct: 181 LAISFLYNPPRIFQAFWKAIEYFIDSKTAQKVHFLYPNNQNSVELMKSFFDMENLPSVFG 240

Query: 241 GKATLTYDHEEFSKMMAIDDLKTAKFWEFSDKPCRHMNGHSGPEVAPEEPMPISVSSA 299
           GKATLTYDHEEFSKMMAIDDLKTAKFWEFSDKPCRHMNGHSGPEVAPEEPMPISVSSA
Sbjct: 241 GKATLTYDHEEFSKMMAIDDLKTAKFWEFSDKPCRHMNGHSGPEVAPEEPMPISVSSA 298

BLAST of Carg00648-RA vs. NCBI nr
Match: XP_022978532.1 (phosphatidylinositol transfer protein 3-like isoform X1 [Cucurbita maxima] >XP_022978533.1 phosphatidylinositol transfer protein 3-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 609.8 bits (1571), Expect = 5.1e-171
Identity = 293/298 (98.32%), Postives = 295/298 (98.99%), Query Frame = 0

Query: 1   MFLFKRRGSQNNGEIDDSSQQDAKINELRAALGHLSGHSLKYCDDACLRRYLVARNWDVH 60
           MFLFKRRGSQNNG IDDSS QDAKINELRAALGHLSGHSLKYCDDACLRRYLVARNWDVH
Sbjct: 1   MFLFKRRGSQNNGAIDDSSPQDAKINELRAALGHLSGHSLKYCDDACLRRYLVARNWDVH 60

Query: 61  KSKKMLDDSLKWRATYKPEEIRWHEVAHEGETGKSFRANFYDRFGRTVLISRPGMQNTNS 120
           KSKKMLDDSLKWRATYKPEEIRWHEVAHEGETGKSFRANFYDRFGR+VLISRPGMQNTNS
Sbjct: 61  KSKKMLDDSLKWRATYKPEEIRWHEVAHEGETGKSFRANFYDRFGRSVLISRPGMQNTNS 120

Query: 121 PEDNVRHVVYVLENTIMNLRDGQEQISWLIDFTGFTINTNISVKAARGIINILQSHYPER 180
           PEDNVRHVVYVLENTIMNLRDGQEQISWLIDFTGFTINTNISVKAARGIINILQSHYPER
Sbjct: 121 PEDNVRHVVYVLENTIMNLRDGQEQISWLIDFTGFTINTNISVKAARGIINILQSHYPER 180

Query: 181 LAISFLYNPPRIFQAFWKAIEYFIDSKTAQKVHFLYPNNQNSVELMKSFFDMENLPSVFG 240
           LAISFLYNPPRIFQAFWKAIEYFIDSKTAQKVHFLYPNNQ+SVELMKSFFDMENLPSVFG
Sbjct: 181 LAISFLYNPPRIFQAFWKAIEYFIDSKTAQKVHFLYPNNQDSVELMKSFFDMENLPSVFG 240

Query: 241 GKATLTYDHEEFSKMMAIDDLKTAKFWEFSDKPCRHMNGHSGPEVAPEEPMPISVSSA 299
           GKATLTYDHEEFSKMM IDDLKTAKFWEFSDKPCRHMNGHSGPEVAPEEPMPISVSSA
Sbjct: 241 GKATLTYDHEEFSKMMTIDDLKTAKFWEFSDKPCRHMNGHSGPEVAPEEPMPISVSSA 298

BLAST of Carg00648-RA vs. NCBI nr
Match: XP_022978534.1 (phosphatidylinositol transfer protein 3-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 609.8 bits (1571), Expect = 5.1e-171
Identity = 293/298 (98.32%), Postives = 295/298 (98.99%), Query Frame = 0

Query: 1   MFLFKRRGSQNNGEIDDSSQQDAKINELRAALGHLSGHSLKYCDDACLRRYLVARNWDVH 60
           MFLFKRRGSQNNG IDDSS QDAKINELRAALGHLSGHSLKYCDDACLRRYLVARNWDVH
Sbjct: 1   MFLFKRRGSQNNGAIDDSSPQDAKINELRAALGHLSGHSLKYCDDACLRRYLVARNWDVH 60

Query: 61  KSKKMLDDSLKWRATYKPEEIRWHEVAHEGETGKSFRANFYDRFGRTVLISRPGMQNTNS 120
           KSKKMLDDSLKWRATYKPEEIRWHEVAHEGETGKSFRANFYDRFGR+VLISRPGMQNTNS
Sbjct: 61  KSKKMLDDSLKWRATYKPEEIRWHEVAHEGETGKSFRANFYDRFGRSVLISRPGMQNTNS 120

Query: 121 PEDNVRHVVYVLENTIMNLRDGQEQISWLIDFTGFTINTNISVKAARGIINILQSHYPER 180
           PEDNVRHVVYVLENTIMNLRDGQEQISWLIDFTGFTINTNISVKAARGIINILQSHYPER
Sbjct: 121 PEDNVRHVVYVLENTIMNLRDGQEQISWLIDFTGFTINTNISVKAARGIINILQSHYPER 180

Query: 181 LAISFLYNPPRIFQAFWKAIEYFIDSKTAQKVHFLYPNNQNSVELMKSFFDMENLPSVFG 240
           LAISFLYNPPRIFQAFWKAIEYFIDSKTAQKVHFLYPNNQ+SVELMKSFFDMENLPSVFG
Sbjct: 181 LAISFLYNPPRIFQAFWKAIEYFIDSKTAQKVHFLYPNNQDSVELMKSFFDMENLPSVFG 240

Query: 241 GKATLTYDHEEFSKMMAIDDLKTAKFWEFSDKPCRHMNGHSGPEVAPEEPMPISVSSA 299
           GKATLTYDHEEFSKMM IDDLKTAKFWEFSDKPCRHMNGHSGPEVAPEEPMPISVSSA
Sbjct: 241 GKATLTYDHEEFSKMMTIDDLKTAKFWEFSDKPCRHMNGHSGPEVAPEEPMPISVSSA 298

BLAST of Carg00648-RA vs. NCBI nr
Match: XP_008440705.1 (PREDICTED: CRAL-TRIO domain-containing protein C23B6.04c-like [Cucumis melo] >XP_008440706.1 PREDICTED: CRAL-TRIO domain-containing protein C23B6.04c-like [Cucumis melo] >XP_016899209.1 PREDICTED: CRAL-TRIO domain-containing protein C23B6.04c-like [Cucumis melo] >XP_016899210.1 PREDICTED: CRAL-TRIO domain-containing protein C23B6.04c-like [Cucumis melo] >XP_016899211.1 PREDICTED: CRAL-TRIO domain-containing protein C23B6.04c-like [Cucumis melo] >XP_016899212.1 PREDICTED: CRAL-TRIO domain-containing protein C23B6.04c-like [Cucumis melo] >XP_016899213.1 PREDICTED: CRAL-TRIO domain-containing protein C23B6.04c-like [Cucumis melo] >XP_016899214.1 PREDICTED: CRAL-TRIO domain-containing protein C23B6.04c-like [Cucumis melo] >XP_016899215.1 PREDICTED: CRAL-TRIO domain-containing protein C23B6.04c-like [Cucumis melo] >XP_016899216.1 PREDICTED: CRAL-TRIO domain-containing protein C23B6.04c-like [Cucumis melo] >XP_016899217.1 PREDICTED: CRAL-TRIO domain-containing protein C23B6.04c-like [Cucumis melo] >XP_016899218.1 PREDICTED: CRAL-TRIO domain-containing protein C23B6.04c-like [Cucumis melo] >XP_016899219.1 PREDICTED: CRAL-TRIO domain-containing protein C23B6.04c-like [Cucumis melo] >XP_016899220.1 PREDICTED: CRAL-TRIO domain-containing protein C23B6.04c-like [Cucumis melo])

HSP 1 Score: 543.9 bits (1400), Expect = 3.4e-151
Identity = 258/297 (86.87%), Postives = 276/297 (92.93%), Query Frame = 0

Query: 1   MFLFKRRGSQNNGEIDDSSQQDAKINELRAALGHLSGHSLKYCDDACLRRYLVARNWDVH 60
           MFL KRRGS NNGE  DSSQQD KI ELR ALGHLSG SLKYC+DACLRRYL ARNWD+H
Sbjct: 1   MFLLKRRGSHNNGETADSSQQDGKITELRGALGHLSGRSLKYCNDACLRRYLAARNWDLH 60

Query: 61  KSKKMLDDSLKWRATYKPEEIRWHEVAHEGETGKSFRANFYDRFGRTVLISRPGMQNTNS 120
           K+KKM++DSLKWRATYKPEEIRWHEVAHEGETGKSFRANFYDRFGRTVLISRPGMQNTNS
Sbjct: 61  KAKKMVEDSLKWRATYKPEEIRWHEVAHEGETGKSFRANFYDRFGRTVLISRPGMQNTNS 120

Query: 121 PEDNVRHVVYVLENTIMNLRDGQEQISWLIDFTGFTINTNISVKAARGIINILQSHYPER 180
           PEDNVRHVVY+LENTI+NLR+GQEQI+WLIDFTGFT+NTNISVKAARGIINILQSHYPER
Sbjct: 121 PEDNVRHVVYLLENTILNLRNGQEQIAWLIDFTGFTMNTNISVKAARGIINILQSHYPER 180

Query: 181 LAISFLYNPPRIFQAFWKAIEYFIDSKTAQKVHFLYPNNQNSVELMKSFFDMENLPSVFG 240
           LAISFLYNPPRIFQAFWKAI YFID KT QKVHF+YPNN++SVELMKSFFDMENLPSVFG
Sbjct: 181 LAISFLYNPPRIFQAFWKAIRYFIDPKTGQKVHFIYPNNKDSVELMKSFFDMENLPSVFG 240

Query: 241 GKATLTYDHEEFSKMMAIDDLKTAKFWEFSDKPCRHMNGHSGPEVAPEEPMPISVSS 298
           GKATLTYDHEEFSKMMA+DD+KTAK WE +DKP  +MNGH G E A EE +PISVSS
Sbjct: 241 GKATLTYDHEEFSKMMAMDDIKTAKLWELNDKPSHNMNGHPGLEAAQEEGIPISVSS 297

BLAST of Carg00648-RA vs. TAIR10
Match: AT1G75170.1 (Sec14p-like phosphatidylinositol transfer family protein)

HSP 1 Score: 363.2 bits (931), Expect = 1.5e-100
Identity = 169/291 (58.08%), Postives = 228/291 (78.35%), Query Frame = 0

Query: 1   MFLFKRRGSQNNGEIDDSSQQDAKINELRAALGHLSGHSLKYCDDACLRRYLVARNWDVH 60
           MF +K   SQ   E  +++ ++AK+ EL+  +G LSG +  YC DACL+RYL ARNW+V 
Sbjct: 1   MFRWK-NSSQTEQEQKEAALREAKMKELKTLIGQLSGRNSLYCSDACLKRYLEARNWNVG 60

Query: 61  KSKKMLDDSLKWRATYKPEEIRWHEVAHEGETGKSFRANFYDRFGRTVLISRPGMQNTNS 120
           K+KKML+++LKWR+++KPEEIRW+EV+ EGETGK ++A F+DR GRTVLI RPG+QNT S
Sbjct: 61  KAKKMLEETLKWRSSFKPEEIRWNEVSGEGETGKVYKAGFHDRHGRTVLILRPGLQNTKS 120

Query: 121 PEDNVRHVVYVLENTIMNLRDGQEQISWLIDFTGFTINTNISVKAARGIINILQSHYPER 180
            E+ ++H+VY++EN I+NL + QEQ+SWLIDFTG++++T++ +K+AR  INILQ+HYPER
Sbjct: 121 LENQMKHLVYLIENAILNLPEDQEQMSWLIDFTGWSMSTSVPIKSARETINILQNHYPER 180

Query: 181 LAISFLYNPPRIFQAFWKAIEYFIDSKTAQKVHFLYPNNQNSVELMKSFFDMENLPSVFG 240
           LA++FLYNPPR+F+AFWK ++YFID+KT  KV F+YP N  SVELM +FFD ENLP+ FG
Sbjct: 181 LAVAFLYNPPRLFEAFWKIVKYFIDAKTFVKVKFVYPKNSESVELMSTFFDEENLPTEFG 240

Query: 241 GKATLTYDHEEFSKMMAIDDLKTAKFW---EFSDKPCRHMNGHSGPEVAPE 289
           GKA L Y++EEFSK M  DD+KTA FW     ++      NG SG E+APE
Sbjct: 241 GKALLQYNYEEFSKQMNQDDVKTANFWGLGHSNNNQLHASNGFSGAEIAPE 290

BLAST of Carg00648-RA vs. TAIR10
Match: AT4G36640.1 (Sec14p-like phosphatidylinositol transfer family protein)

HSP 1 Score: 360.1 bits (923), Expect = 1.3e-99
Identity = 172/297 (57.91%), Postives = 230/297 (77.44%), Query Frame = 0

Query: 3   LFKRRGSQNNGEID-DSSQQDAKINELRAALGHLSGHSLKYCDDACLRRYLVARNWDVHK 62
           +F+RR   N  ++D D SQQD K+ EL++A+G LSGHSL +C DA LRR+L ARNWDV K
Sbjct: 1   MFRRR---NAHQLDNDDSQQDNKVRELKSAIGPLSGHSLVFCSDASLRRFLDARNWDVEK 60

Query: 63  SKKMLDDSLKWRATYKPEEIRWHEVAHEGETGKSFRANFYDRFGRTVLISRPGMQNTNSP 122
           +KKM+ ++LKWR+TYKP+EIRW++VAHEGETGK+ RA+F+DR GR VLI RP MQN+ S 
Sbjct: 61  AKKMIQETLKWRSTYKPQEIRWNQVAHEGETGKASRASFHDRQGRVVLIMRPAMQNSTSQ 120

Query: 123 EDNVRHVVYVLENTIMNLRDGQEQISWLIDFTGFTINTNISVKAARGIINILQSHYPERL 182
           E N+RH+VY+LEN I+NL  GQ+Q+SWLIDFTG+++  N  +K  R II+ILQ++YPERL
Sbjct: 121 EGNIRHLVYLLENAIINLPKGQKQMSWLIDFTGWSMAVNPPMKTTREIIHILQNYYPERL 180

Query: 183 AISFLYNPPRIFQAFWKAIEYFIDSKTAQKVHFLYPNNQNSVELMKSFFDMENLPSVFGG 242
            I+FLYNPPR+FQA ++A +YF+D +TA+KV F+YP ++ S ELM + FD+ENLP  FGG
Sbjct: 181 GIAFLYNPPRLFQAVYRAAKYFLDPRTAEKVKFVYPKDKASDELMTTHFDVENLPKEFGG 240

Query: 243 KATLTYDHEEFSKMMAIDDLKTAKFWEFSDKPCRHMNGHSGPEVAPEEPMPISVSSA 299
           +ATL YDHE+FS+ M  DDLKTAK+W    K     NG S  +V PE  + I+ +++
Sbjct: 241 EATLEYDHEDFSRQMYEDDLKTAKYWGLEGKHYPKTNGFSPSDVVPEPAIEIASAAS 294

BLAST of Carg00648-RA vs. TAIR10
Match: AT1G22180.2 (Sec14p-like phosphatidylinositol transfer family protein)

HSP 1 Score: 254.2 bits (648), Expect = 9.8e-68
Identity = 121/240 (50.42%), Postives = 172/240 (71.67%), Query Frame = 0

Query: 24  KINELRAALGHLSGHSLKYCDDACLRRYLVARNWDVHKSKKMLDDSLKWRATYKPEEIRW 83
           KINE+R  LG L+  S ++C DA + RYL ARN  V K+ KML ++LKWRA YKPEEIRW
Sbjct: 25  KINEVRTLLGPLTEKSSEFCSDAAITRYLAARNGHVKKATKMLKETLKWRAQYKPEEIRW 84

Query: 84  HEVAHEGETGKSFRANFYDRFGRTVLISRPGMQNTNSPEDNVRHVVYVLENTIMNLRDGQ 143
            E+A E ETGK +RAN  D++GRTVL+ RP  QNT S +  +R +VY +EN I+NL D Q
Sbjct: 85  EEIAREAETGKIYRANCTDKYGRTVLVMRPSCQNTKSYKGQIRILVYCMENAILNLPDNQ 144

Query: 144 EQISWLIDFTGFTINTNISVKAARGIINILQSHYPERLAISFLYNPPRIFQAFWKAIEYF 203
           EQ+ WLIDF GF + ++IS+K +R   ++LQ HYPERL ++ +YNPP+IF++F+K ++ F
Sbjct: 145 EQMVWLIDFHGFNM-SHISLKVSRETAHVLQEHYPERLGLAIVYNPPKIFESFYKMVKPF 204

Query: 204 IDSKTAQKVHFLYPNNQNSVELMKSFFDMENLPSVFGGK-ATLTYDHEEFSKMMAIDDLK 263
           ++ KT+ KV F+Y ++  S +L++  FDME L   FGGK +   ++ E++++ M  DDLK
Sbjct: 205 LEPKTSNKVKFVYSDDNLSNKLLEDLFDMEQLEVAFGGKNSDAGFNFEKYAERMREDDLK 263

BLAST of Carg00648-RA vs. TAIR10
Match: AT4G08690.2 (Sec14p-like phosphatidylinositol transfer family protein)

HSP 1 Score: 233.8 bits (595), Expect = 1.4e-61
Identity = 109/245 (44.49%), Postives = 159/245 (64.90%), Query Frame = 0

Query: 18  SSQQDAKINELRAALGHLSGHSLKYCDDACLRRYLVARNWDVHKSKKMLDDSLKWRATYK 77
           + ++ AKI E+R  LG L      +C D  + RYL ARNW V K+ KML ++LKWR  YK
Sbjct: 16  TEEEQAKIEEVRKLLGPLPEKLSSFCSDDAVLRYLRARNWHVKKATKMLKETLKWRVQYK 75

Query: 78  PEEIRWHEVAHEGETGKSFRANFYDRFGRTVLISRPGMQNTNSPEDNVRHVVYVLENTIM 137
           PEEI W EVA E ETGK +R++  D+ GR VLI RP ++N+ S +  +R++VY +EN + 
Sbjct: 76  PEEICWEEVAGEAETGKIYRSSCVDKLGRPVLIMRPSVENSKSVKGQIRYLVYCMENAVQ 135

Query: 138 NLRDGQEQISWLIDFTGFTINTNISVKAARGIINILQSHYPERLAISFLYNPPRIFQAFW 197
           NL  G+EQ+ W+IDF G+++  N+S++  +   ++LQ HYPERLA + LYNPP+ F+ FW
Sbjct: 136 NLPPGEEQMVWMIDFHGYSL-ANVSLRTTKETAHVLQEHYPERLAFAVLYNPPKFFEPFW 195

Query: 198 KAIEYFIDSKTAQKVHFLYPNNQNSVELMKSFFDMENLPSVFGGKATLTYDHEEFSKMMA 257
           K    F++ KT  KV F+Y ++ N+  +M+  FDME +   FGG     ++ E+ S+ M 
Sbjct: 196 KVARPFLEPKTRNKVKFVYSDDPNTKVIMEENFDMEKMELAFGGNDDSGFNIEKHSERMK 255

Query: 258 IDDLK 263
            DD K
Sbjct: 256 EDDKK 259

BLAST of Carg00648-RA vs. TAIR10
Match: AT1G01630.1 (Sec14p-like phosphatidylinositol transfer family protein)

HSP 1 Score: 97.1 bits (240), Expect = 2.0e-20
Identity = 62/208 (29.81%), Postives = 103/208 (49.52%), Query Frame = 0

Query: 41  KYCDDACLRRYLVARNWDVHKSKKMLDDSLKWRATYKPE-EIRWHEVAHEGETGKSFRAN 100
           K  DD  +RR+L AR+ D+ K+  M  + L W+ +  P+  I   E+A++    K     
Sbjct: 46  KEVDDLMIRRFLRARDLDIEKASTMFLNYLTWKRSMLPKGHIPEAEIANDLSHNKMCMQG 105

Query: 101 FYDRFGRTVLISRPGMQNTN--SPEDNVRHVVYVLENTIMNLRDGQEQISWLIDFTGFTI 160
            +D+ GR + ++     N +  +P++  R VVY LE     +  GQE+   + D  G+  
Sbjct: 106 -HDKMGRPIAVAIGNRHNPSKGNPDEFKRFVVYTLEKICARMPRGQEKFVAIGDLQGWGY 165

Query: 161 NTNISVKAARGIINILQSHYPERLAISFLYNPPRIFQAFWKAIEYFIDSKTAQKVHFLYP 220
            +N  ++     ++ LQ  YPERL   ++ + P IF   WK I  FID+ T +K+ F+  
Sbjct: 166 -SNCDIRGYLAALSTLQDCYPERLGKLYIVHAPYIFMTAWKVIYPFIDANTKKKIVFV-- 225

Query: 221 NNQNSVELMKSFFDMENLPSVFGGKATL 246
            N+     +    D   LP ++GGK  L
Sbjct: 226 ENKKLTPTLLEDIDESQLPDIYGGKLPL 249

BLAST of Carg00648-RA vs. Swiss-Prot
Match: sp|Q9UU99|YJX4_SCHPO (CRAL-TRIO domain-containing protein C23B6.04c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPCC23B6.04c PE=1 SV=1)

HSP 1 Score: 115.2 bits (287), Expect = 1.3e-24
Identity = 70/206 (33.98%), Postives = 102/206 (49.51%), Query Frame = 0

Query: 47  CLRRYLVARNWDVHKSKKMLDDSLKWRATYKPEEIRWHEVAHEGETGKSFRANFYDRFGR 106
           C+ RYL A  W V  +KK + D+L WR  +    +   E+  E  TGK      YD+ GR
Sbjct: 637 CILRYLRATKWHVSNAKKRIVDTLVWRRHFGVNNMDPDEIQEENATGKQVLLG-YDKDGR 696

Query: 107 TVLISRPGMQNTNSPEDNVRHVVYVLENTIMNLRDGQEQISWLIDFTGFTINTNISVKAA 166
             L   P  QNT +    +RH+V+ LE  I  +  G E ++ LI+F   +  +N SV   
Sbjct: 697 PCLYLYPARQNTKTSPLQIRHLVFSLECAIDLMPPGVETLALLINFKSSSNRSNPSVGQG 756

Query: 167 RGIINILQSHYPERLAISFLYNPPRIFQAFWKAIEYFIDSKTAQKVHFLYPNNQNSVELM 226
           + ++NILQ+HY ERL  + + N P     F+K I  FID  T +K+ F  P        +
Sbjct: 757 KEVLNILQTHYCERLGRALVINIPWAVWGFFKLISPFIDPITREKLKFNEP--------L 816

Query: 227 KSFFDMENLPSVFGGKATLTYDHEEF 253
             +   + L S FGG     Y HE++
Sbjct: 817 DRYVPKDQLDSNFGGSLHFEYHHEKY 833

BLAST of Carg00648-RA vs. Swiss-Prot
Match: sp|Q55CU8|PITC_DICDI (Phosphatidylinositol transfer protein 3 OS=Dictyostelium discoideum OX=44689 GN=pitC PE=2 SV=1)

HSP 1 Score: 108.6 bits (270), Expect = 1.2e-22
Identity = 76/257 (29.57%), Postives = 129/257 (50.19%), Query Frame = 0

Query: 13  GEIDD-----SSQQDAKINELRAALGHLSGHS-LKYCDDACLRRYLVARNWDVHKSKKML 72
           GEI D     + +Q     E+++    L+  + + +C D C  RYL ARN+ V KS+KML
Sbjct: 35  GEIFDPLKNLNEKQLEAFKEIKSNFSDLTDPTDIAFCTDMCFLRYLRARNYIVSKSEKML 94

Query: 73  DDSLKWRATYKPEEIRWHEVAHEGETGKSFRANFYDRFGRTVLISRP---GMQNTNSPED 132
            D+L+WR  ++P++I+      E  +      N  D+ GR ++ + P    ++N  S E 
Sbjct: 95  RDTLEWRKKFRPQDIQLGGDIREIGSAGCVYVNKRDKKGRPIIFAVPRNDTLKNVPS-EL 154

Query: 133 NVRHVVYVLENTIMNLRD--GQEQISWLIDFTGFTINTNISVKAARGIINILQSHYPERL 192
             +++VY LE     + +  G EQ  +++D+  F  + N+ +K     ++ L  H PER+
Sbjct: 155 KFKNLVYWLEQGFSRMDEPKGIEQFCFIVDYKDFG-SGNMDMKTNLEAMHFLLDHCPERM 214

Query: 193 AISFLYNPPRIFQAFWKAIEYFIDSKTAQKVHFL----YPNNQNSVELMKSFFDMENLPS 252
             S   +PP +F   WK I  F++  T  KV F+        +   EL++ + D+ENL  
Sbjct: 215 GQSLFLDPPALFWFAWKIISPFLNEVTLSKVRFINSKKVDGKRTFAELLE-YVDIENLEQ 274

Query: 253 VFGGKATLTYDHEEFSK 255
             GG     Y+ +E+ K
Sbjct: 275 NLGGNLDYNYNIDEYLK 288

BLAST of Carg00648-RA vs. Swiss-Prot
Match: sp|P53844|PDR17_YEAST (Phosphatidylinositol transfer protein PDR17 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=PDR17 PE=1 SV=1)

HSP 1 Score: 96.7 bits (239), Expect = 4.7e-19
Identity = 74/237 (31.22%), Postives = 108/237 (45.57%), Query Frame = 0

Query: 47  CLRRYLVARNWDVHKSKKMLDDSLKWR----ATYKPEE---IRWHEVAHEGETGKSFRAN 106
           C  RYL A  W+   + K L  +L WR     T+  E+   +   +VA E ETGK     
Sbjct: 94  CFLRYLRANKWNTANAIKGLTKTLVWRREIGLTHGKEDKDPLTADKVAVENETGKQVILG 153

Query: 107 FYDRFGRTVLISRPGMQNTNSPEDNVRHVVYVLENTIMNLRDGQEQISWLIDFTGFTINT 166
           F D   R +   + G QNT S    V+ +VY++E        G E+I+ L+DF  +    
Sbjct: 154 F-DNAKRPLYYMKNGRQNTESSFRQVQELVYMMETATTVAPQGVEKITVLVDFKSYKEPG 213

Query: 167 NISVKA-----ARGIINILQSHYPERLAISFLYNPPRIFQAFWKAIEYFIDSKTAQKVHF 226
            I+ KA     AR  +N++Q HYPERLA   L N P    AF K +  F+D  T  K  F
Sbjct: 214 IITDKAPPISIARMCLNVMQDHYPERLAKCVLINIPWFAWAFLKMMYPFLDPATKAKAIF 273

Query: 227 LYPNNQNSVELMKSFFDMENLPSVFGGKATLTYDHEEF--SKMMAIDDLKTAKFWEF 270
             P         ++  +   L +++ G     Y HE +    +  +DDL+  +F  F
Sbjct: 274 DEP--------FENHIEPSQLDALYNGLLDFKYKHEVYWPDMVKKVDDLRLKRFDRF 321

BLAST of Carg00648-RA vs. Swiss-Prot
Match: sp|P53860|PDR16_YEAST (Phosphatidylinositol transfer protein PDR16 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=PDR16 PE=1 SV=1)

HSP 1 Score: 94.4 bits (233), Expect = 2.3e-18
Identity = 66/221 (29.86%), Postives = 103/221 (46.61%), Query Frame = 0

Query: 47  CLRRYLVARNWDVHKSKKMLDDSLKWRATY--------KPEEIRWHEVAHEGETGKSFRA 106
           C  RYL A  W +      +  +L WR  +          ++I    VA E E+GK    
Sbjct: 89  CFLRYLRATKWVLKDCIDRITMTLAWRREFGISHLGEEHGDKITADLVAVENESGKQVIL 148

Query: 107 NFYDRFGRTVLISRPGMQNTNSPEDNVRHVVYVLENTIMNLRDGQEQISWLIDFTGFTI- 166
             Y+   R +L  +PG QNT +    V+H+V++LE  I  +  GQ+ ++ LIDF  +   
Sbjct: 149 G-YENDARPILYLKPGRQNTKTSHRQVQHLVFMLERVIDFMPAGQDSLALLIDFKDYPDV 208

Query: 167 -----NTNI-SVKAARGIINILQSHYPERLAISFLYNPPRIFQAFWKAIEYFIDSKTAQK 226
                N+ I  +   + +++ILQ+HYPERL  + L N P +   F K I  FID  T +K
Sbjct: 209 PKVPGNSKIPPIGVGKEVLHILQTHYPERLGKALLTNIPWLAWTFLKLIHPFIDPLTREK 268

Query: 227 VHFLYPNNQNSVELMKSFFDMENLPSVFGGKATLTYDHEEF 253
           + F  P           +     L S++GG     Y+H+ +
Sbjct: 269 LVFDEP--------FVKYVPKNELDSLYGGDLKFKYNHDVY 300

BLAST of Carg00648-RA vs. Swiss-Prot
Match: sp|F4JYJ3|SFH11_ARATH (Phosphatidylinositol/phosphatidylcholine transfer protein SFH11 OS=Arabidopsis thaliana OX=3702 GN=SFH11 PE=3 SV=1)

HSP 1 Score: 80.1 bits (196), Expect = 4.6e-14
Identity = 79/266 (29.70%), Postives = 126/266 (47.37%), Query Frame = 0

Query: 5   KRRGSQNNGEIDDSSQQDAKINELRAALGHLSGHSLKYCDDACLRRYLVARNWDVHKSKK 64
           K   S NN E+ ++         L    GHL     K+ D   LRR+L  R++D+ KSK+
Sbjct: 17  KEEQSPNNEEMVEA------FRNLLLLHGHLPD---KHGDHNTLRRFLKMRDFDLEKSKE 76

Query: 65  MLDDSLKWRATYKPEEI-RWHEVAHEGETGKSFRANFY--DRFGRTVLISRPGMQNTNS- 124
              + +KWR  YK + I +  +    GE  K +   F+  D+ GR + I R GM + N+ 
Sbjct: 77  AFLNYMKWRVDYKVDLISQKFKFEEYGEVKKHYPHGFHKVDKTGRPIYIERLGMTDLNAF 136

Query: 125 --PEDNVRHVVYVL--ENTIMNLR---------DGQEQISWLIDFTGFTINTNISVKAAR 184
                  R+V Y +  +   M+LR               + ++D +G  + +N S K AR
Sbjct: 137 LKATTIERYVNYHIKEQEKTMSLRYPACSIASDKHVSSTTTILDVSGVGM-SNFS-KPAR 196

Query: 185 GIINILQ----SHYPERLAISFLYNPPRIFQAFWKAIEYFIDSKTAQKVHFLYPNNQNSV 244
            +   +Q    ++YPE L   F+ N    F+  W A++ F+D++T  KV  L PN     
Sbjct: 197 SLFMEIQKIDSNYYPETLHRLFVVNASSGFRMLWLALKTFLDARTLAKVQVLGPNYLG-- 256

Query: 245 ELMKSFFDMENLPSVFGGKATLTYDH 250
           EL+++  +  NLP+  GG  T + DH
Sbjct: 257 ELLEA-IEPSNLPTFLGGNCTCS-DH 267

BLAST of Carg00648-RA vs. TrEMBL
Match: tr|A0A1S4DU33|A0A1S4DU33_CUCME (CRAL-TRIO domain-containing protein C23B6.04c-like OS=Cucumis melo OX=3656 GN=LOC103485046 PE=4 SV=1)

HSP 1 Score: 543.9 bits (1400), Expect = 2.3e-151
Identity = 258/297 (86.87%), Postives = 276/297 (92.93%), Query Frame = 0

Query: 1   MFLFKRRGSQNNGEIDDSSQQDAKINELRAALGHLSGHSLKYCDDACLRRYLVARNWDVH 60
           MFL KRRGS NNGE  DSSQQD KI ELR ALGHLSG SLKYC+DACLRRYL ARNWD+H
Sbjct: 1   MFLLKRRGSHNNGETADSSQQDGKITELRGALGHLSGRSLKYCNDACLRRYLAARNWDLH 60

Query: 61  KSKKMLDDSLKWRATYKPEEIRWHEVAHEGETGKSFRANFYDRFGRTVLISRPGMQNTNS 120
           K+KKM++DSLKWRATYKPEEIRWHEVAHEGETGKSFRANFYDRFGRTVLISRPGMQNTNS
Sbjct: 61  KAKKMVEDSLKWRATYKPEEIRWHEVAHEGETGKSFRANFYDRFGRTVLISRPGMQNTNS 120

Query: 121 PEDNVRHVVYVLENTIMNLRDGQEQISWLIDFTGFTINTNISVKAARGIINILQSHYPER 180
           PEDNVRHVVY+LENTI+NLR+GQEQI+WLIDFTGFT+NTNISVKAARGIINILQSHYPER
Sbjct: 121 PEDNVRHVVYLLENTILNLRNGQEQIAWLIDFTGFTMNTNISVKAARGIINILQSHYPER 180

Query: 181 LAISFLYNPPRIFQAFWKAIEYFIDSKTAQKVHFLYPNNQNSVELMKSFFDMENLPSVFG 240
           LAISFLYNPPRIFQAFWKAI YFID KT QKVHF+YPNN++SVELMKSFFDMENLPSVFG
Sbjct: 181 LAISFLYNPPRIFQAFWKAIRYFIDPKTGQKVHFIYPNNKDSVELMKSFFDMENLPSVFG 240

Query: 241 GKATLTYDHEEFSKMMAIDDLKTAKFWEFSDKPCRHMNGHSGPEVAPEEPMPISVSS 298
           GKATLTYDHEEFSKMMA+DD+KTAK WE +DKP  +MNGH G E A EE +PISVSS
Sbjct: 241 GKATLTYDHEEFSKMMAMDDIKTAKLWELNDKPSHNMNGHPGLEAAQEEGIPISVSS 297

BLAST of Carg00648-RA vs. TrEMBL
Match: tr|A0A0A0KM57|A0A0A0KM57_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G502640 PE=4 SV=1)

HSP 1 Score: 534.3 bits (1375), Expect = 1.8e-148
Identity = 253/298 (84.90%), Postives = 278/298 (93.29%), Query Frame = 0

Query: 1   MFLFKRRGSQNNGEIDDSSQQDAKINELRAALGHLSGHSLKYCDDACLRRYLVARNWDVH 60
           MFL KRRGS NNGE DD S QD KI EL+ A+G+LSG S+KYC+DACLRRYL ARNWD++
Sbjct: 1   MFLLKRRGSHNNGETDDPSLQDGKIIELKGAMGNLSGRSIKYCNDACLRRYLAARNWDLN 60

Query: 61  KSKKMLDDSLKWRATYKPEEIRWHEVAHEGETGKSFRANFYDRFGRTVLISRPGMQNTNS 120
           K+KKML+DSLKWRATYKPEEIRW+EVAHEGETGKSFRANFYDRFGRTVLISRPGMQNTNS
Sbjct: 61  KAKKMLEDSLKWRATYKPEEIRWNEVAHEGETGKSFRANFYDRFGRTVLISRPGMQNTNS 120

Query: 121 PEDNVRHVVYVLENTIMNLRDGQEQISWLIDFTGFTINTNISVKAARGIINILQSHYPER 180
           PEDNVRHVVY+LENTI+NLR+GQEQI+WLIDFTGFT+NTNISVKAARGIINILQSHYPER
Sbjct: 121 PEDNVRHVVYLLENTILNLRNGQEQIAWLIDFTGFTLNTNISVKAARGIINILQSHYPER 180

Query: 181 LAISFLYNPPRIFQAFWKAIEYFIDSKTAQKVHFLYPNNQNSVELMKSFFDMENLPSVFG 240
           LA+SFLYNPPRIFQAFWKAI YFID  T QKV+F+YPNN++SVELMKSFFDMENLPSVFG
Sbjct: 181 LAVSFLYNPPRIFQAFWKAIRYFIDPNTGQKVNFIYPNNKDSVELMKSFFDMENLPSVFG 240

Query: 241 GKATLTYDHEEFSKMMAIDDLKTAKFWEFSDKPCRHMNGHSGPEVAPEEPMPISVSSA 299
           GKATLTYDHEEFSKMMA+DD+KTAKFWE +DKP  +MNGHSG EVA EE +PISVSSA
Sbjct: 241 GKATLTYDHEEFSKMMAMDDIKTAKFWEVNDKPSHNMNGHSGLEVAQEEAIPISVSSA 298

BLAST of Carg00648-RA vs. TrEMBL
Match: tr|A0A2P5FXL4|A0A2P5FXL4_9ROSA (Cellular retinaldehyde binding/alpha-tocopherol transport OS=Trema orientalis OX=63057 GN=TorRG33x02_015200 PE=4 SV=1)

HSP 1 Score: 444.1 bits (1141), Expect = 2.4e-121
Identity = 207/297 (69.70%), Postives = 251/297 (84.51%), Query Frame = 0

Query: 3   LFKRRGSQNNGEIDDSSQQDAKINELRAALGHLSGHSLKYCDDACLRRYLVARNWDVHKS 62
           +F RR    N  +D+S++++ K+ ELR ALG L+G SLKYC D CLRRYL ARNW+V KS
Sbjct: 1   MFLRRKQSQNHHVDESAEKEEKVKELRDALGTLTGRSLKYCTDGCLRRYLEARNWNVDKS 60

Query: 63  KKMLDDSLKWRATYKPEEIRWHEVAHEGETGKSFRANFYDRFGRTVLISRPGMQNTNSPE 122
           KKMLD++LKWRATYKPEE RW EVAHEGETGK  +ANF+DR GR VLI RPGMQNTNSPE
Sbjct: 61  KKMLDETLKWRATYKPEETRWSEVAHEGETGKVSKANFHDRLGRPVLIMRPGMQNTNSPE 120

Query: 123 DNVRHVVYVLENTIMNLRDGQEQISWLIDFTGFTINTNISVKAARGIINILQSHYPERLA 182
            N+RH+VY++EN ++NL +GQEQ+SWL+DFTGFT+NTN+SVK AR IINILQ+HYPERLA
Sbjct: 121 GNIRHLVYLIENGVLNLPEGQEQMSWLVDFTGFTLNTNVSVKTARDIINILQNHYPERLA 180

Query: 183 ISFLYNPPRIFQAFWKAIEYFIDSKTAQKVHFLYPNNQNSVELMKSFFDMENLPSVFGGK 242
           + FLYNPPRIFQAF+KA+ YF+D KTAQKV F+YP N++SVELMK+FFD++NLP+ FGGK
Sbjct: 181 VVFLYNPPRIFQAFYKAVRYFLDPKTAQKVKFVYPKNKDSVELMKTFFDIDNLPNEFGGK 240

Query: 243 AT--LTYDHEEFSKMMAIDDLKTAKFWEFSDKPCRHMNGHSGPEVAPEEPMPISVSS 298
           AT  L YDHEEFS+MMA+DD+KTAKFW F DKP    NGH GPEVAP EP+P++ S+
Sbjct: 241 ATASLKYDHEEFSRMMALDDVKTAKFWGFDDKPSLKTNGHFGPEVAP-EPIPLAPST 296

BLAST of Carg00648-RA vs. TrEMBL
Match: tr|A0A2P4II64|A0A2P4II64_QUESU (Cral-trio domain-containing protein c23b6.04c OS=Quercus suber OX=58331 GN=CFP56_29019 PE=4 SV=1)

HSP 1 Score: 443.0 bits (1138), Expect = 5.4e-121
Identity = 207/294 (70.41%), Postives = 252/294 (85.71%), Query Frame = 0

Query: 1   MFLFKRRGSQNNGEIDDSSQQDAKINELRAALGHLSGHSLKYCDDACLRRYLVARNWDVH 60
           MFL +R     +   D + ++DAK++ELRAALG LSG  LKYC+DACLRRYL ARNW+V 
Sbjct: 1   MFLLRRNTQTQSHPEDLNLEKDAKVSELRAALGPLSGRCLKYCNDACLRRYLEARNWNVD 60

Query: 61  KSKKMLDDSLKWRATYKPEEIRWHEVAHEGETGKSFRANFYDRFGRTVLISRPGMQNTNS 120
           K+KKML+++LKWRATYKPEEIRWHE+AHEGETGK+FR+NF+D  GRTVLI RPGMQNTNS
Sbjct: 61  KAKKMLEETLKWRATYKPEEIRWHEIAHEGETGKAFRSNFHDMLGRTVLIMRPGMQNTNS 120

Query: 121 PEDNVRHVVYVLENTIMNLRDGQEQISWLIDFTGFTINTNISVKAARGIINILQSHYPER 180
           PEDNVRH+VY+LEN+I+NL +GQEQ+SWLIDFTGF+++TNIS+KAAR IINILQ+HYPER
Sbjct: 121 PEDNVRHIVYLLENSILNLPEGQEQMSWLIDFTGFSLSTNISIKAARDIINILQNHYPER 180

Query: 181 LAISFLYNPPRIFQAFWKAIEYFIDSKTAQKVHFLYPNNQNSVELMKSFFDMENLPSVFG 240
           LAI+ LYNPP+IFQAF+KA++YF+D KTAQKV F+YPN+++S   MK+ FD+ENLP  FG
Sbjct: 181 LAIALLYNPPKIFQAFYKAVKYFLDPKTAQKVSFVYPNSKSSDNFMKTLFDLENLPCEFG 240

Query: 241 GKATLTYDHEEFSKMMAIDDLKTAKFWEFSDKPCRHMNGHSGPEVAPEEPMPIS 295
           GKATL YDHEEFS+MMA DD+KTAK W F ++P   MNGHSG  V  +EPMPIS
Sbjct: 241 GKATLKYDHEEFSRMMAEDDVKTAKLWGFDERPHHIMNGHSGAAVV-QEPMPIS 293

BLAST of Carg00648-RA vs. TrEMBL
Match: tr|M5VRG1|M5VRG1_PRUPE (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_7G154300 PE=4 SV=1)

HSP 1 Score: 442.6 bits (1137), Expect = 7.1e-121
Identity = 205/294 (69.73%), Postives = 253/294 (86.05%), Query Frame = 0

Query: 1   MFLFKRRGSQNNGEIDDSSQQDAKINELRAALGHLSGHSLKYCDDACLRRYLVARNWDVH 60
           M+L +R+  + + E DDS+Q+DAK+ ELRAALG LSG +LKYC DACLRRYL AR+W++ 
Sbjct: 1   MYLLRRQAQKPSPE-DDSAQKDAKVKELRAALGPLSGRNLKYCTDACLRRYLEARSWNLD 60

Query: 61  KSKKMLDDSLKWRATYKPEEIRWHEVAHEGETGKSFRANFYDRFGRTVLISRPGMQNTNS 120
           K+KKM++++L+WRATYKPEE RWHE+AHEGETGK  RANF+DR GRTVLI RP MQNTNS
Sbjct: 61  KAKKMVEETLRWRATYKPEETRWHEIAHEGETGKVSRANFHDRLGRTVLIMRPAMQNTNS 120

Query: 121 PEDNVRHVVYVLENTIMNLRDGQEQISWLIDFTGFTINTNISVKAARGIINILQSHYPER 180
           PE N+RH+VY++EN I+NL +GQEQ+SWL+DF+GF++NTN+SVK AR II ILQ+HYPER
Sbjct: 121 PEGNIRHLVYLIENGILNLPEGQEQMSWLVDFSGFSLNTNVSVKTARDIIYILQNHYPER 180

Query: 181 LAISFLYNPPRIFQAFWKAIEYFIDSKTAQKVHFLYPNNQNSVELMKSFFDMENLPSVFG 240
           LA++FLYNPPRIFQAFWKA++YF+D KT QKV F+YP  + SVELMK+FFD+ENLPS FG
Sbjct: 181 LAVAFLYNPPRIFQAFWKAVKYFLDPKTFQKVKFVYPKGKESVELMKTFFDVENLPSEFG 240

Query: 241 GKATLTYDHEEFSKMMAIDDLKTAKFWEFSDKPCRHMNGHSGPEVAPEEPMPIS 295
           G+ATL YDHEEFS+MMA DD+KTAKFW F +KPCR  NG SG EV P EP+PI+
Sbjct: 241 GQATLKYDHEEFSRMMAEDDVKTAKFWGFDEKPCRIKNGLSGAEVVP-EPLPIA 292

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023543859.11.9e-17399.66phosphatidylinositol transfer protein 3-like [Cucurbita pepo subsp. pepo] >XP_02... [more]
XP_022950390.11.7e-17198.66CRAL-TRIO domain-containing protein C23B6.04c-like [Cucurbita moschata] >XP_0229... [more]
XP_022978532.15.1e-17198.32phosphatidylinositol transfer protein 3-like isoform X1 [Cucurbita maxima] >XP_0... [more]
XP_022978534.15.1e-17198.32phosphatidylinositol transfer protein 3-like isoform X2 [Cucurbita maxima][more]
XP_008440705.13.4e-15186.87PREDICTED: CRAL-TRIO domain-containing protein C23B6.04c-like [Cucumis melo] >XP... [more]
Match NameE-valueIdentityDescription
AT1G75170.11.5e-10058.08Sec14p-like phosphatidylinositol transfer family protein[more]
AT4G36640.11.3e-9957.91Sec14p-like phosphatidylinositol transfer family protein[more]
AT1G22180.29.8e-6850.42Sec14p-like phosphatidylinositol transfer family protein[more]
AT4G08690.21.4e-6144.49Sec14p-like phosphatidylinositol transfer family protein[more]
AT1G01630.12.0e-2029.81Sec14p-like phosphatidylinositol transfer family protein[more]
Match NameE-valueIdentityDescription
sp|Q9UU99|YJX4_SCHPO1.3e-2433.98CRAL-TRIO domain-containing protein C23B6.04c OS=Schizosaccharomyces pombe (stra... [more]
sp|Q55CU8|PITC_DICDI1.2e-2229.57Phosphatidylinositol transfer protein 3 OS=Dictyostelium discoideum OX=44689 GN=... [more]
sp|P53844|PDR17_YEAST4.7e-1931.22Phosphatidylinositol transfer protein PDR17 OS=Saccharomyces cerevisiae (strain ... [more]
sp|P53860|PDR16_YEAST2.3e-1829.86Phosphatidylinositol transfer protein PDR16 OS=Saccharomyces cerevisiae (strain ... [more]
sp|F4JYJ3|SFH11_ARATH4.6e-1429.70Phosphatidylinositol/phosphatidylcholine transfer protein SFH11 OS=Arabidopsis t... [more]
Match NameE-valueIdentityDescription
tr|A0A1S4DU33|A0A1S4DU33_CUCME2.3e-15186.87CRAL-TRIO domain-containing protein C23B6.04c-like OS=Cucumis melo OX=3656 GN=LO... [more]
tr|A0A0A0KM57|A0A0A0KM57_CUCSA1.8e-14884.90Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G502640 PE=4 SV=1[more]
tr|A0A2P5FXL4|A0A2P5FXL4_9ROSA2.4e-12169.70Cellular retinaldehyde binding/alpha-tocopherol transport OS=Trema orientalis OX... [more]
tr|A0A2P4II64|A0A2P4II64_QUESU5.4e-12170.41Cral-trio domain-containing protein c23b6.04c OS=Quercus suber OX=58331 GN=CFP56... [more]
tr|M5VRG1|M5VRG1_PRUPE7.1e-12169.73Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_7G154300 PE=4 SV=1[more]
The following terms have been associated with this mRNA:
Vocabulary: INTERPRO
TermDefinition
IPR036273CRAL/TRIO_N_dom_sf
IPR036865CRAL-TRIO_dom_sf
IPR011074CRAL/TRIO_N_dom
IPR001251CRAL-TRIO_dom
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0003674 molecular_function

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Carg00648Carg00648gene


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Carg00648-RACarg00648-RA-proteinpolypeptide


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg00648-RA.CDS.5Carg00648-RA.CDS.5CDS
Carg00648-RA.CDS.4Carg00648-RA.CDS.4CDS
Carg00648-RA.CDS.3Carg00648-RA.CDS.3CDS
Carg00648-RA.CDS.2Carg00648-RA.CDS.2CDS
Carg00648-RA.CDS.1Carg00648-RA.CDS.1CDS


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg00648-RA.five_prime_UTR.1Carg00648-RA.five_prime_UTR.1five_prime_UTR


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg00648-RA.exon.5Carg00648-RA.exon.5exon
Carg00648-RA.exon.4Carg00648-RA.exon.4exon
Carg00648-RA.exon.3Carg00648-RA.exon.3exon
Carg00648-RA.exon.2Carg00648-RA.exon.2exon
Carg00648-RA.exon.1Carg00648-RA.exon.1exon


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001251CRAL-TRIO lipid binding domainSMARTSM00516sec14_4coord: 89..244
e-value: 2.3E-37
score: 140.1
IPR001251CRAL-TRIO lipid binding domainPFAMPF00650CRAL_TRIOcoord: 101..242
e-value: 1.0E-30
score: 106.4
IPR001251CRAL-TRIO lipid binding domainPROSITEPS50191CRAL_TRIOcoord: 101..247
score: 20.041
IPR001251CRAL-TRIO lipid binding domainCDDcd00170SEC14coord: 102..242
e-value: 7.90604E-33
score: 118.642
IPR011074CRAL/TRIO, N-terminal domainSMARTSM01100CRAL_TRIO_N_2coord: 44..69
e-value: 4.6E-7
score: 39.5
IPR011074CRAL/TRIO, N-terminal domainPFAMPF03765CRAL_TRIO_Ncoord: 42..67
e-value: 4.6E-6
score: 26.6
IPR036865CRAL-TRIO lipid binding domain superfamilyGENE3DG3DSA:3.40.525.10coord: 8..273
e-value: 1.1E-75
score: 256.7
IPR036865CRAL-TRIO lipid binding domain superfamilySUPERFAMILYSSF52087CRAL/TRIO domaincoord: 83..256
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 278..298
NoneNo IPR availablePANTHERPTHR10174RETINALDEHYDE BINDING PROTEIN-RELATEDcoord: 12..289
NoneNo IPR availablePANTHERPTHR10174:SF206SUBFAMILY NOT NAMEDcoord: 12..289
IPR036273CRAL/TRIO, N-terminal domain superfamilySUPERFAMILYSSF46938CRAL/TRIO N-terminal domaincoord: 16..85