CSPI07G15680.1 (mRNA) Wild cucumber (PI 183967)

NameCSPI07G15680.1
TypemRNA
OrganismCucumis sativus (Wild cucumber (PI 183967))
DescriptionFatty acid desaturase family protein
LocationChr7 : 14297483 .. 14300155 (-)
Sequence length993
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAGGAGCATCCAAAGAAGACCCAAAGGTGATGGTGATGAGCTCTCCTTCAAGGCTCTTTAAGAGAAAAAAGTGGACAAAGTTTGATAAAAACGTTGCCTTTTACTTTCTTCTTTTGCATCTACTTAGTATTTTGGCACCATTATTCTATTTTAGTTGGGGTTGTTTGTTGCTTTCCTTGGGATTAGCCAATCTAACCGGGATGGCAATTAGTGTTTCGTATCATAGAAATCTTGCACATCGAAGCTTCAAACTTCCAAAATGGCTCGAGTATTCTCTTGCTTATATTGCAGCTCATGCAATTCAGGTTTGATACTTTGAATCCCTTATGCTCTAACTAAAAATGGACTAAACTACGAGTAAAACATGAATTACACATTTTGATTTTATACTCAATTTGAGGTTGGTACTCTATGACACATTATTTAATTTTACTTCACCTTTTTGAATTAATCAATAATTTAATATGATATTAGAATAAATGTTTCACAAGGTTCTTTTGTATTCAAAACCATTGCAATGTCATTACCTCCTTAATTAATATTGATTCTCACACATTTCAATCCCACAAAAGTTAGAGAAATGATTTTGAATCTAAAAACGTCTTGTACACACATTTCAATCCCACAAAAGTTAGAGAAATGATTTTGAATCTAAAAACGAACAGTGTTGATCATTACATTATACATGAAAGAATTATTCTTATACACATTACTTAGACATCAAATCATAAAAGGATTGAGTAGTGTTTAGTAATTGCCTACATAACATTAAAAAAGATTAAATTAGAGACTAATCCTATCATATTTTTTATACGGTGTTTTGTTAACATTTGGTTGGTTTCTTCAATTTTAGGGTGATCCAATTGATTGGGTGAGCACACACAGATGTCACCATCGATTTGTTGATTCAGAACGAGATCCACATAGTCCTATTTATGGATTTTGGTTCAGCCAAATGACGTGGTTTTTTGATTCGTATAACTTGACGAGAAAAGTTTGTCCAAACTATTTTAATAATTATCAAAAGGTGGAGAGAAACATCTTGATGTTTTACATGAAACATGGATATCCAGACAATGTGACAGATTTGGAGAAACAAAAGTTCTATAGGTTTATACATAAAACATATTGGCTTCATCCACTTGCTCTTTCACTCTTGCTCTATGCACTTGGAGGACTTCCTTTCATCATTTGGGGAGTGGTAAAATATAATTATTATTCCCTTTCCATTTCCTCTTTTAATTATTAAGGTTATAAACTCTATTTGTTTGAAGGTTCAAAATTTTCTTTCAACTTTTCTAGAAGAAGGAAATTTTATTTTTTTTCATTAATAAATTAAGTTGTTGATTTTTTTACATTGTTATATTTGATTTATTCATTACATGTTTTTAGGCAATCTACACAACAGACAATTTAAGTGTTTTTTTAAAAAATAAATGAAAGAAAAATAATATGTATACATGTATAATACTTATTTTTTTTCTTTTTACATAAAGGTAAAAAATTTATTTGACAATTAATTATTGATTTATACTATATATAAAGTTAAAATTATATATAAGTACTTAAACCTAAACTCTCAATTCTAAAATTTATACTAATCTAATTCTTCATCTTTTAAAAATATTTAGTAGGTTTTTGGACTATTTGTATGTTTAAACAAGTCATACTTTCAAATTTCTTTTGTCCAGAAGATTTATGAATTTAAAAATAAATAAATTTTATAGTCTCATTCGATAATGATTTTGTTTTAATTTTTTATAATGGTTTTCATTTTCTTCAAATAAACATTAAAACCTAATTTTCAAGTAAAACACTAGATCGTTGATAATCAGTCTAAAGAATTGTGTTTGATTATGATTATAATTATTAGATTATACTAGAGAGATGGATGATCTTTCCACCATATATTTGTTTAATTATATATGTTTTTCATTGAAATATATATTTTATTTATGAAGTGTATTTTATTAAACTATTGACAGTGTGTGAGGTTCGTACTGAGCTTGCATTTCACATCTATGATAAATTCAGTATGTCATATTTGGGGAAACCAACCATGGAATACTGGGGATTTATCTAAGAACAATTGGTAAGACATACACTTTTCTTATTATATCTCTTTTCTTTTTTATTCTTTTCTTTTACCAATTTGCTAGTGAAAAATGGGTTTTATATTAATTAATTAATGATTGATACATCAATTTTCATATTTATATCATCAGTCCAGTTATATAAAATTTGAATATTCTATATCAACCCTTTACCATTTGTCTCGAAATAATTTTGCTTTTTAAAGTAGGAAGTAAATTTTTTAAACCTTCAGGACAATTACAAAGTTTAAATAGTTTGTTTTACAACTGTCAAACTATTATAGTATTTAGATAATCTTGACTTGACAAAGTTTAAATATATAGTTCATTGTTTCATTTTTAATTAGGATTGTTGCTATATTTACCTTTGGAGAAGGATGGCACAATAACCACCATGCTTTTGAGTATTCAGCACGACATGGTCATGAATGGTGGCAAATTGATAATGGTTGGTATATTATCAAGTTCCTTCAAGTCATTGGATTAGCCACCAATGTTAAATTACCTTCTGAAAAGCAAAAAATGGCTGCTTTAAACAAATCAAAACCCACCTTTCTTAGGCCTATGATTGATTGA

mRNA sequence

ATGGAAGGAGCATCCAAAGAAGACCCAAAGGTGATGGTGATGAGCTCTCCTTCAAGGCTCTTTAAGAGAAAAAAGTGGACAAAGTTTGATAAAAACGTTGCCTTTTACTTTCTTCTTTTGCATCTACTTAGTATTTTGGCACCATTATTCTATTTTAGTTGGGGTTGTTTGTTGCTTTCCTTGGGATTAGCCAATCTAACCGGGATGGCAATTAGTGTTTCGTATCATAGAAATCTTGCACATCGAAGCTTCAAACTTCCAAAATGGCTCGAGTATTCTCTTGCTTATATTGCAGCTCATGCAATTCAGGGTGATCCAATTGATTGGGTGAGCACACACAGATGTCACCATCGATTTGTTGATTCAGAACGAGATCCACATAGTCCTATTTATGGATTTTGGTTCAGCCAAATGACGTGGTTTTTTGATTCGTATAACTTGACGAGAAAAGTTTGTCCAAACTATTTTAATAATTATCAAAAGGTGGAGAGAAACATCTTGATGTTTTACATGAAACATGGATATCCAGACAATGTGACAGATTTGGAGAAACAAAAGTTCTATAGGTTTATACATAAAACATATTGGCTTCATCCACTTGCTCTTTCACTCTTGCTCTATGCACTTGGAGGACTTCCTTTCATCATTTGGGGAGTGTGTGTGAGGTTCGTACTGAGCTTGCATTTCACATCTATGATAAATTCAGTATGTCATATTTGGGGAAACCAACCATGGAATACTGGGGATTTATCTAAGAACAATTGGATTGTTGCTATATTTACCTTTGGAGAAGGATGGCACAATAACCACCATGCTTTTGAGTATTCAGCACGACATGGTCATGAATGGTGGCAAATTGATAATGGTTGGTATATTATCAAGTTCCTTCAAGTCATTGGATTAGCCACCAATGTTAAATTACCTTCTGAAAAGCAAAAAATGGCTGCTTTAAACAAATCAAAACCCACCTTTCTTAGGCCTATGATTGATTGA

Coding sequence (CDS)

ATGGAAGGAGCATCCAAAGAAGACCCAAAGGTGATGGTGATGAGCTCTCCTTCAAGGCTCTTTAAGAGAAAAAAGTGGACAAAGTTTGATAAAAACGTTGCCTTTTACTTTCTTCTTTTGCATCTACTTAGTATTTTGGCACCATTATTCTATTTTAGTTGGGGTTGTTTGTTGCTTTCCTTGGGATTAGCCAATCTAACCGGGATGGCAATTAGTGTTTCGTATCATAGAAATCTTGCACATCGAAGCTTCAAACTTCCAAAATGGCTCGAGTATTCTCTTGCTTATATTGCAGCTCATGCAATTCAGGGTGATCCAATTGATTGGGTGAGCACACACAGATGTCACCATCGATTTGTTGATTCAGAACGAGATCCACATAGTCCTATTTATGGATTTTGGTTCAGCCAAATGACGTGGTTTTTTGATTCGTATAACTTGACGAGAAAAGTTTGTCCAAACTATTTTAATAATTATCAAAAGGTGGAGAGAAACATCTTGATGTTTTACATGAAACATGGATATCCAGACAATGTGACAGATTTGGAGAAACAAAAGTTCTATAGGTTTATACATAAAACATATTGGCTTCATCCACTTGCTCTTTCACTCTTGCTCTATGCACTTGGAGGACTTCCTTTCATCATTTGGGGAGTGTGTGTGAGGTTCGTACTGAGCTTGCATTTCACATCTATGATAAATTCAGTATGTCATATTTGGGGAAACCAACCATGGAATACTGGGGATTTATCTAAGAACAATTGGATTGTTGCTATATTTACCTTTGGAGAAGGATGGCACAATAACCACCATGCTTTTGAGTATTCAGCACGACATGGTCATGAATGGTGGCAAATTGATAATGGTTGGTATATTATCAAGTTCCTTCAAGTCATTGGATTAGCCACCAATGTTAAATTACCTTCTGAAAAGCAAAAAATGGCTGCTTTAAACAAATCAAAACCCACCTTTCTTAGGCCTATGATTGATTGA
BLAST of CSPI07G15680.1 vs. Swiss-Prot
Match: ADS3_ARATH (Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic OS=Arabidopsis thaliana GN=ADS3 PE=1 SV=2)

HSP 1 Score: 359.8 bits (922), Expect = 3.3e-98
Identity = 169/304 (55.59%), Postives = 212/304 (69.74%), Query Frame = 1

Query: 11  VMVMSSPSRLFKRKKWTKFDKNVAFYFLLLHLLSILAPLFYFSWGCLLLSLGLANLTGM- 70
           V+V      ++  ++W   D       L +HLLS+LAP F F+W  + ++ GL  +TG+ 
Sbjct: 83  VLVKKKEKVVWWEREWKAMDFGAVAVVLSMHLLSLLAP-FQFNWRAVSVAFGLYIVTGLL 142

Query: 71  AISVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVDSERDPHSP 130
            I++S+HRNL+H++FKLPKWLEY  AY  A A+QG+PIDWVSTHR HH+F DS+RDPHSP
Sbjct: 143 GITLSFHRNLSHKAFKLPKWLEYLFAYCGAQALQGNPIDWVSTHRYHHQFCDSDRDPHSP 202

Query: 131 IYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNILMFYMKHGYPDNVTDLEKQKFYR 190
           + GFWFS M W FD+  +T++                       G P+NV DLEKQ FYR
Sbjct: 203 LDGFWFSHMNWMFDTNTITQRC----------------------GEPNNVGDLEKQPFYR 262

Query: 191 FIHKTYWLHPLALSLLLYALGGLPFIIWGVCVRFVLSLHFTSMINSVCHIWGNQPWNTGD 250
           F+  TY LHPLAL++ LYA+GG PFI+WG+ VR V   H T ++NS CH+WG Q WNTGD
Sbjct: 263 FLRTTYILHPLALAVALYAMGGFPFIVWGMGVRIVWVYHITWLVNSACHVWGKQAWNTGD 322

Query: 251 LSKNNWIVAIFTFGEGWHNNHHAFEYSARHGHEWWQIDNGWYIIKFLQVIGLATNVKLPS 310
           LSKNNW VA   FGEGWHNNHHAFE+SARHG EWWQ+D  WY++KFLQ IGLAT+VKLPS
Sbjct: 323 LSKNNWWVAALAFGEGWHNNHHAFEFSARHGLEWWQLDMTWYVVKFLQAIGLATDVKLPS 363

Query: 311 EKQK 314
           E QK
Sbjct: 383 EAQK 363

BLAST of CSPI07G15680.1 vs. Swiss-Prot
Match: ADS32_ARATH (Probable lipid desaturase ADS3.2, chloroplastic OS=Arabidopsis thaliana GN=ADS3.2 PE=2 SV=3)

HSP 1 Score: 324.7 bits (831), Expect = 1.2e-87
Identity = 153/301 (50.83%), Postives = 194/301 (64.45%), Query Frame = 1

Query: 12  MVMSSPSRLFKRKKWTKFDKNVAFYFLLLHLLSILAPLFYFSWGCLLLSLGLANLTGMAI 71
           +V+    R F  + WT +D +    F+  HLLS+LAP FYFSW    +   L  + G+ I
Sbjct: 77  VVVVRTKRSFWERSWTSWDVSKLVIFVGTHLLSLLAP-FYFSWEAFWVFPWLVFINGICI 136

Query: 72  SVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVDSERDPHSPIY 131
           ++SYHRNL+HRSF LPKWLEY  AY    A QGDPI+WVS HR HH+  +++RDPHSP  
Sbjct: 137 TLSYHRNLSHRSFDLPKWLEYLFAYGGVLAFQGDPIEWVSNHRYHHKHCETQRDPHSPTQ 196

Query: 132 GFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNILMFYMKHGYPDNVTDLEKQKFYRFI 191
           GFWFS M W FD+ ++                          G  +NV DL +Q FYRF+
Sbjct: 197 GFWFSHMAWIFDTSSILENC----------------------GGEENVDDLVRQPFYRFL 256

Query: 192 HKTYWLHPLALSLLLYALGGLPFIIWGVCVRFVLSLHFTSMINSVCHIWGNQPWNTGDLS 251
            +T  LH +A S L Y  GG+P ++WG+ +   + LH T ++NSVCHIWG + WNT D S
Sbjct: 257 QRTVLLHMMAYSFLFYFCGGMPLLVWGIGITIAVRLHLTFLVNSVCHIWGTRAWNTSDFS 316

Query: 252 KNNWIVAIFTFGEGWHNNHHAFEYSARHGHEWWQIDNGWYIIKFLQVIGLATNVKLPSEK 311
           KNNW VAI T GEGWHNNHHAFE+SARHG EWWQ+D  W +I+FL+ IGLATNVKLP+E 
Sbjct: 317 KNNWWVAILTLGEGWHNNHHAFEFSARHGLEWWQLDITWCLIRFLEAIGLATNVKLPTET 354

Query: 312 Q 313
           Q
Sbjct: 377 Q 354

BLAST of CSPI07G15680.1 vs. Swiss-Prot
Match: ADS1_ARATH (Delta-9 acyl-lipid desaturase 1 OS=Arabidopsis thaliana GN=ADS1 PE=2 SV=1)

HSP 1 Score: 315.5 bits (807), Expect = 7.1e-85
Identity = 151/300 (50.33%), Postives = 193/300 (64.33%), Query Frame = 1

Query: 14  MSSPSRLFKRKKWTKFDKNVAFYFLLLHLLSILAPLFYFSWGCLLLSLGLANLTGMAISV 73
           M    R    +KW + D   AF  L +H L +LAP F F+W  L ++L +  + G+ I+V
Sbjct: 22  MGRKKRAMWERKWKRLDIVKAFASLFVHFLCLLAP-FNFTWPALRVALIVYTVGGLGITV 81

Query: 74  SYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVDSERDPHSPIYGF 133
           SYHRNLAHRSFK+PKWLEY  AY    AIQGDPIDWVSTHR HH+F DS+RDPHSP  GF
Sbjct: 82  SYHRNLAHRSFKVPKWLEYFFAYCGLLAIQGDPIDWVSTHRYHHQFTDSDRDPHSPNEGF 141

Query: 134 WFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNILMFYMKHGYPDNVTDLEKQKFYRFIHK 193
           WFS + W FD+  L  K                       G   NV DL++Q +Y+F+ +
Sbjct: 142 WFSHLLWLFDTGYLVEKC----------------------GRRTNVEDLKRQWYYKFLQR 201

Query: 194 TYWLHPLALSLLLYALGGLPFIIWGVCVRFVLSLHFTSMINSVCHIWGNQPWNTGDLSKN 253
           T   H L    LLY  GGL F+ WG+ +   +  H T +INS+CH+WG++ W T D S+N
Sbjct: 202 TVLYHILTFGFLLYYFGGLSFLTWGMGIGVAMEHHVTCLINSLCHVWGSRTWKTNDTSRN 261

Query: 254 NWIVAIFTFGEGWHNNHHAFEYSARHGHEWWQIDNGWYIIKFLQVIGLATNVKLPSEKQK 313
            W +++F+FGE WHNNHHAFE SAR G EWWQID  WYI++FL++IGLAT+VKLPSE Q+
Sbjct: 262 VWWLSVFSFGESWHNNHHAFESSARQGLEWWQIDISWYIVRFLEIIGLATDVKLPSESQR 298

BLAST of CSPI07G15680.1 vs. Swiss-Prot
Match: ADS2_ARATH (Delta-9 acyl-lipid desaturase 2 OS=Arabidopsis thaliana GN=ADS2 PE=2 SV=2)

HSP 1 Score: 314.7 bits (805), Expect = 1.2e-84
Identity = 151/294 (51.36%), Postives = 192/294 (65.31%), Query Frame = 1

Query: 20  LFKRKKWTKFDKNVAFYFLLLHLLSILAPLFYFSWGCLLLSLGLANLTGMAISVSYHRNL 79
           +F  ++W + D         +H L++LAP FYF+W  L ++     + G+ I+VSYHRNL
Sbjct: 30  VFWDRRWRRLDYVKFSASFTVHSLALLAP-FYFTWSALWVTFLFYTIGGLGITVSYHRNL 89

Query: 80  AHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVDSERDPHSPIYGFWFSQMT 139
           AHRSFK+PKWLEY LAY A  AIQGDPIDWVSTHR HH+F DSERDPHSP  GFWFS + 
Sbjct: 90  AHRSFKVPKWLEYLLAYCALLAIQGDPIDWVSTHRYHHQFTDSERDPHSPKEGFWFSHLL 149

Query: 140 WFFDSYNLTRKVCPNYFNNYQKVERNILMFYMKHGYPDNVTDLEKQKFYRFIHKTYWLHP 199
           W +DS  L  K                       G   NV DL++Q FYRF+ KT   H 
Sbjct: 150 WIYDSAYLVSKC----------------------GRRANVEDLKRQWFYRFLQKTVLFHI 209

Query: 200 LALSLLLYALGGLPFIIWGVCVRFVLSLHFTSMINSVCHIWGNQPWNTGDLSKNNWIVAI 259
           L L   L+ LGG+ F+ WG+ V   L +H T +INS+CHIWG + W T D S+N W +++
Sbjct: 210 LGLGFFLFYLGGMSFVTWGMGVGAALEVHVTCLINSLCHIWGTRTWKTNDTSRNVWWLSV 269

Query: 260 FTFGEGWHNNHHAFEYSARHGHEWWQIDNGWYIIKFLQVIGLATNVKLPSEKQK 314
           F+FGE WHNNHHAFE SAR G EWWQID  WYI++F ++IGLAT+VK+P+E Q+
Sbjct: 270 FSFGESWHNNHHAFESSARQGLEWWQIDISWYIVRFFEIIGLATDVKVPTEAQR 300

BLAST of CSPI07G15680.1 vs. Swiss-Prot
Match: ICO5D_LIMDO (Icosanoyl-CoA 5-desaturase (Fragment) OS=Limnanthes douglasii PE=1 SV=1)

HSP 1 Score: 308.1 bits (788), Expect = 1.1e-82
Identity = 156/311 (50.16%), Postives = 191/311 (61.41%), Query Frame = 1

Query: 12  MVMSSPSRLFKRKKWTKFDKNVAFYFLLLHLLSILAPLFYFSWGCLLLSLGLANLTG--M 71
           +V+   ++ F R+KW   D   A   L+LH L+ +AP FYFSW    +S  L       +
Sbjct: 67  VVLERKAKGFWRRKWNPRDIQNAVTLLVLHALAAMAP-FYFSWDAFWISFILLGFASGVL 126

Query: 72  AISVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVDSERDPHSP 131
            I++ +HR L H  FKLPK +EY  AY  + A+QGDP++WVS HR HH+FVD+ERD HSP
Sbjct: 127 GITLCFHRCLTHGGFKLPKLVEYFFAYCGSLALQGDPMEWVSNHRYHHQFVDTERDVHSP 186

Query: 132 IYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNILMFYMKHGYPDNVTDLEKQKFYR 191
             GFWF  + W  D      K                     + G  +NV DL+KQ FYR
Sbjct: 187 TQGFWFCHIGWVLDKDLFVEK---------------------RGGRRNNVNDLKKQAFYR 246

Query: 192 FIHKTYWLHPLALSLLLYALGGLPFIIWGVCVRFVLSLHFTSMINSVCHIWGNQPWNTGD 251
           F+ KTY  H LAL  LLY +GG P+I+WG+  R V   H T  INSVCH WG +PWNTGD
Sbjct: 247 FLQKTYMYHQLALIALLYYVGGFPYIVWGMGFRLVFMFHSTFAINSVCHKWGGRPWNTGD 306

Query: 252 LSKNNWIVAIFTFGEGWHNNHHAFEYSARHGHEWWQIDNGWYIIKFLQVIGLATNVKLPS 311
           LS NN  VA+  FGEGWHNNHHAFE SARHG EWW+ID  WY+I+ LQ IGLATNVKLP+
Sbjct: 307 LSTNNMFVALCAFGEGWHNNHHAFEQSARHGLEWWEIDVTWYVIRTLQAIGLATNVKLPT 355

Query: 312 EKQKMAALNKS 321
           E QK     KS
Sbjct: 367 EAQKQKLKAKS 355

BLAST of CSPI07G15680.1 vs. TrEMBL
Match: A0A0A0KAB1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G376370 PE=3 SV=1)

HSP 1 Score: 636.0 bits (1639), Expect = 2.6e-179
Identity = 295/330 (89.39%), Postives = 309/330 (93.64%), Query Frame = 1

Query: 1   MEGASKEDPKVMVMSSPSRLFKRKKWTKFDKNVAFYFLLLHLLSILAPLFYFSWGCLLLS 60
           M+  SKE PK MVM SP RLF+RKKWTKFD+NVAFYFL LHLL ILAP FYFSW CLLL+
Sbjct: 1   MDEVSKEKPKEMVMKSPPRLFRRKKWTKFDRNVAFYFLSLHLLCILAP-FYFSWTCLLLA 60

Query: 61  LGLANLTGMAISVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFV 120
           LGL+ LTGM ISVSYHRNLAHRSFKLPKWLEYSLAYIA HAIQGDPIDWVSTHRCHHRFV
Sbjct: 61  LGLSYLTGMGISVSYHRNLAHRSFKLPKWLEYSLAYIATHAIQGDPIDWVSTHRCHHRFV 120

Query: 121 DSERDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNILMFYMKHGYPDNVT 180
           DS RDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNI MFYMKHGYPDNVT
Sbjct: 121 DSARDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNIFMFYMKHGYPDNVT 180

Query: 181 DLEKQKFYRFIHKTYWLHPLALSLLLYALGGLPFIIWGVCVRFVLSLHFTSMINSVCHIW 240
           DLEKQKFYRFIHKTYWLHPLALSLLLYA+GGLPFIIWG+CVRFV SLH TSM+NSVCHIW
Sbjct: 181 DLEKQKFYRFIHKTYWLHPLALSLLLYAIGGLPFIIWGMCVRFVYSLHVTSMVNSVCHIW 240

Query: 241 GNQPWNTGDLSKNNWIVAIFTFGEGWHNNHHAFEYSARHGHEWWQIDNGWYIIKFLQVIG 300
           GNQPWNTGDLSKNNW+VA+ TFGEGWHNNHHAFEYSARHGHEWWQID GWY+I+FLQVIG
Sbjct: 241 GNQPWNTGDLSKNNWMVALLTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYLIRFLQVIG 300

Query: 301 LATNVKLPSEKQKMAALNKSKPTFLRPMID 331
           LATNVKLPSEKQK+AALNKSKP FL+ MID
Sbjct: 301 LATNVKLPSEKQKLAALNKSKPIFLKLMID 329

BLAST of CSPI07G15680.1 vs. TrEMBL
Match: A0A0A0K8P8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G377870 PE=3 SV=1)

HSP 1 Score: 570.9 bits (1470), Expect = 1.0e-159
Identity = 261/262 (99.62%), Postives = 261/262 (99.62%), Query Frame = 1

Query: 69  MAISVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVDSERDPHS 128
           MAISVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVDSERDPHS
Sbjct: 1   MAISVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVDSERDPHS 60

Query: 129 PIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNILMFYMKHGYPDNVTDLEKQKFY 188
           PIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNI MFYMKHGYPDNVTDLEKQKFY
Sbjct: 61  PIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNIFMFYMKHGYPDNVTDLEKQKFY 120

Query: 189 RFIHKTYWLHPLALSLLLYALGGLPFIIWGVCVRFVLSLHFTSMINSVCHIWGNQPWNTG 248
           RFIHKTYWLHPLALSLLLYALGGLPFIIWGVCVRFVLSLHFTSMINSVCHIWGNQPWNTG
Sbjct: 121 RFIHKTYWLHPLALSLLLYALGGLPFIIWGVCVRFVLSLHFTSMINSVCHIWGNQPWNTG 180

Query: 249 DLSKNNWIVAIFTFGEGWHNNHHAFEYSARHGHEWWQIDNGWYIIKFLQVIGLATNVKLP 308
           DLSKNNWIVAIFTFGEGWHNNHHAFEYSARHGHEWWQIDNGWYIIKFLQVIGLATNVKLP
Sbjct: 181 DLSKNNWIVAIFTFGEGWHNNHHAFEYSARHGHEWWQIDNGWYIIKFLQVIGLATNVKLP 240

Query: 309 SEKQKMAALNKSKPTFLRPMID 331
           SEKQKMAALNKSKPTFLRPMID
Sbjct: 241 SEKQKMAALNKSKPTFLRPMID 262

BLAST of CSPI07G15680.1 vs. TrEMBL
Match: A0A0A0KVH2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G006060 PE=3 SV=1)

HSP 1 Score: 446.8 bits (1148), Expect = 2.3e-122
Identity = 202/323 (62.54%), Postives = 247/323 (76.47%), Query Frame = 1

Query: 1   MEGASKEDPKVMVMSSPSRLFKRKKWTKFDKNVAFYFLLLHLLSILAPLFYFSWGCLLLS 60
           M+ + +E+PK MV   P R F R+ WT  DK VA+  L +HLL I AP F F+W    ++
Sbjct: 18  MDESKQEEPKKMV--KPPRSFWRRNWTTLDKRVAYIILFVHLLCIFAP-FQFNWSAFRVT 77

Query: 61  LGLANLTGM-AISVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRF 120
           + L  +TG+  +++SYHRNLAH+SF+LPKWLEYS AY   H +QGDP+DWVSTHRCHH+F
Sbjct: 78  VALIVITGLFGMTISYHRNLAHKSFQLPKWLEYSFAYCGVHCLQGDPLDWVSTHRCHHQF 137

Query: 121 VDSERDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNILMFYMKHGYPDNV 180
           VDSE+DPHSPI GFW S M W FDSY LT KVCP Y ++ +K+ERN+ + + KHG PDNV
Sbjct: 138 VDSEKDPHSPINGFWISHMMWLFDSYTLTNKVCPKYSSDLKKIERNMFVIFTKHGKPDNV 197

Query: 181 TDLEKQKFYRFIHKTYWLHPLALSLLLYALGGLPFIIWGVCVRFVLSLHFTSMINSVCHI 240
            DLEKQ+FYRFIHKTY LH LAL+++LY++GG+PF IWG+CVR V   H T M+NSVCHI
Sbjct: 198 GDLEKQRFYRFIHKTYMLHHLALAIILYSIGGVPFFIWGMCVRIVAVFHSTFMVNSVCHI 257

Query: 241 WGNQPWNTGDLSKNNWIVAIFTFGEGWHNNHHAFEYSARHGHEWWQIDNGWYIIKFLQVI 300
           WG QPW TGDLS+NNW+V + +FGEGWHNNHHAF YSAR G EWWQ+D GWY+IKFLQVI
Sbjct: 258 WGKQPWRTGDLSRNNWLVGLTSFGEGWHNNHHAFSYSARLGLEWWQLDAGWYVIKFLQVI 317

Query: 301 GLATNVKLPSEKQKMAALNKSKP 323
           GLATNVKLPS     +     KP
Sbjct: 318 GLATNVKLPSPTHMQSLAMDHKP 337

BLAST of CSPI07G15680.1 vs. TrEMBL
Match: A0A0A0KYH4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G006130 PE=3 SV=1)

HSP 1 Score: 441.0 bits (1133), Expect = 1.2e-120
Identity = 198/304 (65.13%), Postives = 241/304 (79.28%), Query Frame = 1

Query: 10  KVMVMSSPSRLFKRKKWTKFDKNVAFYFLLLHLLSILAPLFYFSWGCLLLSLGLANLTGM 69
           K   M    R FKR+K+TK DK + F   ++H+  I AP F+F+W    ++  L  +TG+
Sbjct: 6   KPKAMGKSPRSFKRRKYTKLDKIIFFSLFIVHIACIFAP-FHFTWPAFCVAFALYFITGL 65

Query: 70  AISVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVDSERDPHSP 129
            ISVSYHRNLAH+SFKLPK +EY LAY AAHA+QGDPIDWVSTHRCHH+FVD+E+DPHSP
Sbjct: 66  CISVSYHRNLAHKSFKLPKSMEYFLAYCAAHALQGDPIDWVSTHRCHHQFVDTEKDPHSP 125

Query: 130 IYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNILMFYMKHGYPDNVTDLEKQKFYR 189
           I GFWFS +TW FDSYNLT+KVCPNYF ++QKV+R+I +++ KHG PDNV DLEKQ FYR
Sbjct: 126 IEGFWFSHITWLFDSYNLTKKVCPNYFTDFQKVDRSIFIWFSKHGRPDNVRDLEKQTFYR 185

Query: 190 FIHKTYWLHPLALSLLLYALGGLPFIIWGVCVRFVLSLHFTSMINSVCHIWGNQPWNTGD 249
           FIHKTY+LH + L++LLY +GGLPF+IWG CVR V+ +H T M+NSVCHIWG + WNT D
Sbjct: 186 FIHKTYFLHHILLAILLYQVGGLPFLIWGTCVRIVVLMHVTFMVNSVCHIWGKRRWNTKD 245

Query: 250 LSKNNWIVAIFTFGEGWHNNHHAFEYSARHGHEWWQIDNGWYIIKFLQVIGLATNVKLPS 309
           LS+NNW+V +  FGEGWHNNHHAFEYSAR G +WWQID GWY I+ LQ IG+A NVKLPS
Sbjct: 246 LSRNNWLVGLLAFGEGWHNNHHAFEYSARFGLQWWQIDFGWYTIRVLQAIGVAKNVKLPS 305

Query: 310 EKQK 314
              K
Sbjct: 306 TIHK 308

BLAST of CSPI07G15680.1 vs. TrEMBL
Match: A0A0A0LTL5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G227450 PE=3 SV=1)

HSP 1 Score: 404.4 bits (1038), Expect = 1.3e-109
Identity = 188/313 (60.06%), Postives = 237/313 (75.72%), Query Frame = 1

Query: 12  MVMSSPSRLFKRKKWTKFDKNVAFYFLLLHLLSILAPLFYFSWGCLLLSLGLANLTG-MA 71
           MV  +    F ++KWT  DK +A +F+ LH + ILAP F+F+W    ++  L  +TG   
Sbjct: 4   MVKKADLITFGQRKWTNRDKYMAAFFIFLHFICILAP-FHFNWNAFWVAFALYIITGCFG 63

Query: 72  ISVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVDSERDPHSPI 131
           I++SYHRNL+HRSF+LPKWLEY  AY  A A QGDPIDWVSTHRCHH+FVD+E DPHSPI
Sbjct: 64  ITISYHRNLSHRSFRLPKWLEYFFAYCGALAFQGDPIDWVSTHRCHHQFVDTENDPHSPI 123

Query: 132 YGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNILMFYMKHGYPDNVTDLEKQKFYRF 191
            GFWFS   W  DS  LTR+VCP YF +++  E+ I    +K+G P+NV DLEKQ FYRF
Sbjct: 124 QGFWFSYFIWILDSNALTRRVCPQYFIDHKDTEKTIFTVVLKYGRPNNVGDLEKQFFYRF 183

Query: 192 IHKTYWLHPLALSLLLYALGGLPFIIWGVCVRFVLSLHFTSMINSVCHIWGNQPWNTGDL 251
           + KTY+LH LAL++LLYA+GG PF+IWG  VR + ++H T M+NS+CH +GNQPWNTGDL
Sbjct: 184 LRKTYFLHQLALAILLYAVGGTPFLIWGAFVRTIGTIHVTFMVNSICHTFGNQPWNTGDL 243

Query: 252 SKNNWIVAIFTFGEGWHNNHHAFEYSARHGHEWWQIDNGWYIIKFLQVIGLATNVKLPSE 311
           S+N W + + +FGEGWHNNHHAFEYSAR G EWWQID  WYII FLQVIGLAT VK+PS+
Sbjct: 244 SRNTWWMCLISFGEGWHNNHHAFEYSARQGLEWWQIDISWYIIWFLQVIGLATEVKVPSQ 303

Query: 312 --KQKMAALNKSK 322
             KQ++ AL+++K
Sbjct: 304 SHKQRLQALDQTK 315

BLAST of CSPI07G15680.1 vs. TAIR10
Match: AT3G15850.1 (AT3G15850.1 fatty acid desaturase 5)

HSP 1 Score: 359.8 bits (922), Expect = 1.8e-99
Identity = 169/304 (55.59%), Postives = 212/304 (69.74%), Query Frame = 1

Query: 11  VMVMSSPSRLFKRKKWTKFDKNVAFYFLLLHLLSILAPLFYFSWGCLLLSLGLANLTGM- 70
           V+V      ++  ++W   D       L +HLLS+LAP F F+W  + ++ GL  +TG+ 
Sbjct: 83  VLVKKKEKVVWWEREWKAMDFGAVAVVLSMHLLSLLAP-FQFNWRAVSVAFGLYIVTGLL 142

Query: 71  AISVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVDSERDPHSP 130
            I++S+HRNL+H++FKLPKWLEY  AY  A A+QG+PIDWVSTHR HH+F DS+RDPHSP
Sbjct: 143 GITLSFHRNLSHKAFKLPKWLEYLFAYCGAQALQGNPIDWVSTHRYHHQFCDSDRDPHSP 202

Query: 131 IYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNILMFYMKHGYPDNVTDLEKQKFYR 190
           + GFWFS M W FD+  +T++                       G P+NV DLEKQ FYR
Sbjct: 203 LDGFWFSHMNWMFDTNTITQRC----------------------GEPNNVGDLEKQPFYR 262

Query: 191 FIHKTYWLHPLALSLLLYALGGLPFIIWGVCVRFVLSLHFTSMINSVCHIWGNQPWNTGD 250
           F+  TY LHPLAL++ LYA+GG PFI+WG+ VR V   H T ++NS CH+WG Q WNTGD
Sbjct: 263 FLRTTYILHPLALAVALYAMGGFPFIVWGMGVRIVWVYHITWLVNSACHVWGKQAWNTGD 322

Query: 251 LSKNNWIVAIFTFGEGWHNNHHAFEYSARHGHEWWQIDNGWYIIKFLQVIGLATNVKLPS 310
           LSKNNW VA   FGEGWHNNHHAFE+SARHG EWWQ+D  WY++KFLQ IGLAT+VKLPS
Sbjct: 323 LSKNNWWVAALAFGEGWHNNHHAFEFSARHGLEWWQLDMTWYVVKFLQAIGLATDVKLPS 363

Query: 311 EKQK 314
           E QK
Sbjct: 383 EAQK 363

BLAST of CSPI07G15680.1 vs. TAIR10
Match: AT3G15870.1 (AT3G15870.1 Fatty acid desaturase family protein)

HSP 1 Score: 324.7 bits (831), Expect = 6.6e-89
Identity = 153/301 (50.83%), Postives = 194/301 (64.45%), Query Frame = 1

Query: 12  MVMSSPSRLFKRKKWTKFDKNVAFYFLLLHLLSILAPLFYFSWGCLLLSLGLANLTGMAI 71
           +V+    R F  + WT +D +    F+  HLLS+LAP FYFSW    +   L  + G+ I
Sbjct: 77  VVVVRTKRSFWERSWTSWDVSKLVIFVGTHLLSLLAP-FYFSWEAFWVFPWLVFINGICI 136

Query: 72  SVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVDSERDPHSPIY 131
           ++SYHRNL+HRSF LPKWLEY  AY    A QGDPI+WVS HR HH+  +++RDPHSP  
Sbjct: 137 TLSYHRNLSHRSFDLPKWLEYLFAYGGVLAFQGDPIEWVSNHRYHHKHCETQRDPHSPTQ 196

Query: 132 GFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNILMFYMKHGYPDNVTDLEKQKFYRFI 191
           GFWFS M W FD+ ++                          G  +NV DL +Q FYRF+
Sbjct: 197 GFWFSHMAWIFDTSSILENC----------------------GGEENVDDLVRQPFYRFL 256

Query: 192 HKTYWLHPLALSLLLYALGGLPFIIWGVCVRFVLSLHFTSMINSVCHIWGNQPWNTGDLS 251
            +T  LH +A S L Y  GG+P ++WG+ +   + LH T ++NSVCHIWG + WNT D S
Sbjct: 257 QRTVLLHMMAYSFLFYFCGGMPLLVWGIGITIAVRLHLTFLVNSVCHIWGTRAWNTSDFS 316

Query: 252 KNNWIVAIFTFGEGWHNNHHAFEYSARHGHEWWQIDNGWYIIKFLQVIGLATNVKLPSEK 311
           KNNW VAI T GEGWHNNHHAFE+SARHG EWWQ+D  W +I+FL+ IGLATNVKLP+E 
Sbjct: 317 KNNWWVAILTLGEGWHNNHHAFEFSARHGLEWWQLDITWCLIRFLEAIGLATNVKLPTET 354

Query: 312 Q 313
           Q
Sbjct: 377 Q 354

BLAST of CSPI07G15680.1 vs. TAIR10
Match: AT1G06080.1 (AT1G06080.1 delta 9 desaturase 1)

HSP 1 Score: 315.5 bits (807), Expect = 4.0e-86
Identity = 151/300 (50.33%), Postives = 193/300 (64.33%), Query Frame = 1

Query: 14  MSSPSRLFKRKKWTKFDKNVAFYFLLLHLLSILAPLFYFSWGCLLLSLGLANLTGMAISV 73
           M    R    +KW + D   AF  L +H L +LAP F F+W  L ++L +  + G+ I+V
Sbjct: 22  MGRKKRAMWERKWKRLDIVKAFASLFVHFLCLLAP-FNFTWPALRVALIVYTVGGLGITV 81

Query: 74  SYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVDSERDPHSPIYGF 133
           SYHRNLAHRSFK+PKWLEY  AY    AIQGDPIDWVSTHR HH+F DS+RDPHSP  GF
Sbjct: 82  SYHRNLAHRSFKVPKWLEYFFAYCGLLAIQGDPIDWVSTHRYHHQFTDSDRDPHSPNEGF 141

Query: 134 WFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNILMFYMKHGYPDNVTDLEKQKFYRFIHK 193
           WFS + W FD+  L  K                       G   NV DL++Q +Y+F+ +
Sbjct: 142 WFSHLLWLFDTGYLVEKC----------------------GRRTNVEDLKRQWYYKFLQR 201

Query: 194 TYWLHPLALSLLLYALGGLPFIIWGVCVRFVLSLHFTSMINSVCHIWGNQPWNTGDLSKN 253
           T   H L    LLY  GGL F+ WG+ +   +  H T +INS+CH+WG++ W T D S+N
Sbjct: 202 TVLYHILTFGFLLYYFGGLSFLTWGMGIGVAMEHHVTCLINSLCHVWGSRTWKTNDTSRN 261

Query: 254 NWIVAIFTFGEGWHNNHHAFEYSARHGHEWWQIDNGWYIIKFLQVIGLATNVKLPSEKQK 313
            W +++F+FGE WHNNHHAFE SAR G EWWQID  WYI++FL++IGLAT+VKLPSE Q+
Sbjct: 262 VWWLSVFSFGESWHNNHHAFESSARQGLEWWQIDISWYIVRFLEIIGLATDVKLPSESQR 298

BLAST of CSPI07G15680.1 vs. TAIR10
Match: AT2G31360.1 (AT2G31360.1 16:0delta9 desaturase 2)

HSP 1 Score: 314.7 bits (805), Expect = 6.8e-86
Identity = 151/294 (51.36%), Postives = 192/294 (65.31%), Query Frame = 1

Query: 20  LFKRKKWTKFDKNVAFYFLLLHLLSILAPLFYFSWGCLLLSLGLANLTGMAISVSYHRNL 79
           +F  ++W + D         +H L++LAP FYF+W  L ++     + G+ I+VSYHRNL
Sbjct: 30  VFWDRRWRRLDYVKFSASFTVHSLALLAP-FYFTWSALWVTFLFYTIGGLGITVSYHRNL 89

Query: 80  AHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVDSERDPHSPIYGFWFSQMT 139
           AHRSFK+PKWLEY LAY A  AIQGDPIDWVSTHR HH+F DSERDPHSP  GFWFS + 
Sbjct: 90  AHRSFKVPKWLEYLLAYCALLAIQGDPIDWVSTHRYHHQFTDSERDPHSPKEGFWFSHLL 149

Query: 140 WFFDSYNLTRKVCPNYFNNYQKVERNILMFYMKHGYPDNVTDLEKQKFYRFIHKTYWLHP 199
           W +DS  L  K                       G   NV DL++Q FYRF+ KT   H 
Sbjct: 150 WIYDSAYLVSKC----------------------GRRANVEDLKRQWFYRFLQKTVLFHI 209

Query: 200 LALSLLLYALGGLPFIIWGVCVRFVLSLHFTSMINSVCHIWGNQPWNTGDLSKNNWIVAI 259
           L L   L+ LGG+ F+ WG+ V   L +H T +INS+CHIWG + W T D S+N W +++
Sbjct: 210 LGLGFFLFYLGGMSFVTWGMGVGAALEVHVTCLINSLCHIWGTRTWKTNDTSRNVWWLSV 269

Query: 260 FTFGEGWHNNHHAFEYSARHGHEWWQIDNGWYIIKFLQVIGLATNVKLPSEKQK 314
           F+FGE WHNNHHAFE SAR G EWWQID  WYI++F ++IGLAT+VK+P+E Q+
Sbjct: 270 FSFGESWHNNHHAFESSARQGLEWWQIDISWYIVRFFEIIGLATDVKVPTEAQR 300

BLAST of CSPI07G15680.1 vs. TAIR10
Match: AT1G06100.1 (AT1G06100.1 Fatty acid desaturase family protein)

HSP 1 Score: 302.8 bits (774), Expect = 2.7e-82
Identity = 152/295 (51.53%), Postives = 189/295 (64.07%), Query Frame = 1

Query: 19  RLFKRKKWTKFDKNVAFYFLLLHLLSILAPLFYFSWGCLLLSLGLANLTGMAISVSYHRN 78
           R +  +KWT+FD   A     +HLL +LAP F + W      + LA LT + I+ SYHRN
Sbjct: 21  RAYVLRKWTQFDVGRASTVGTVHLLCLLAP-FNYKWEAFRFGIILAILTNLCITFSYHRN 80

Query: 79  LAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVDSERDPHSPIYGFWFSQM 138
           L HRSFKLPKWLEY  AY A  A+QGDP+DWVS HR HH+F DS+RDPHSPI GFWFS +
Sbjct: 81  LTHRSFKLPKWLEYPFAYSALLALQGDPLDWVSIHRFHHQFTDSDRDPHSPIEGFWFSHV 140

Query: 139 TWFFDSYNLTRKVCPNYFNNYQKVERNILMFYMKHGYPDNVTDLEKQKFYRFIHKTYWLH 198
            W FD+ +  R+ C                     G  +NV DL++Q FYRF+ KT  LH
Sbjct: 141 LWIFDT-DYIREKC---------------------GRRNNVMDLKQQWFYRFLKKTLVLH 200

Query: 199 PLALSLLLYALGGLPFIIWGVCVRFVLSLHFTSMINSVCHIWGNQPWNTGDLSKNNWIVA 258
            LA   L+Y  GGLP++ W V    V+  H T ++NS CHI G+Q W T D S+N W +A
Sbjct: 201 ILAFWTLIYLWGGLPYLTWTVGFGGVIGYHGTWLVNSACHICGSQAWQTNDTSRNVWWLA 260

Query: 259 IFTFGEGWHNNHHAFEYSARHGHEWWQIDNGWYIIKFLQVIGLATNVKLPSEKQK 314
           + T GE WHNNHHAFE SARHG EW+Q+D  WY+I F Q +GLATNVKLP++ QK
Sbjct: 261 LLTMGESWHNNHHAFETSARHGLEWYQLDITWYLIWFFQALGLATNVKLPTDAQK 292

BLAST of CSPI07G15680.1 vs. NCBI nr
Match: gi|778730379|ref|XP_011659770.1| (PREDICTED: palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Cucumis sativus])

HSP 1 Score: 698.7 bits (1802), Expect = 4.8e-198
Identity = 328/330 (99.39%), Postives = 328/330 (99.39%), Query Frame = 1

Query: 1   MEGASKEDPKVMVMSSPSRLFKRKKWTKFDKNVAFYFLLLHLLSILAPLFYFSWGCLLLS 60
           MEGASKEDPK MVMSSPSRLFKRKKWTKFDKNVAFYFLLLHLLSILAPLFYFSWGCLLLS
Sbjct: 1   MEGASKEDPKEMVMSSPSRLFKRKKWTKFDKNVAFYFLLLHLLSILAPLFYFSWGCLLLS 60

Query: 61  LGLANLTGMAISVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFV 120
           LGLANLTGMAISVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFV
Sbjct: 61  LGLANLTGMAISVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFV 120

Query: 121 DSERDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNILMFYMKHGYPDNVT 180
           DSERDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNI MFYMKHGYPDNVT
Sbjct: 121 DSERDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNIFMFYMKHGYPDNVT 180

Query: 181 DLEKQKFYRFIHKTYWLHPLALSLLLYALGGLPFIIWGVCVRFVLSLHFTSMINSVCHIW 240
           DLEKQKFYRFIHKTYWLHPLALSLLLYALGGLPFIIWGVCVRFVLSLHFTSMINSVCHIW
Sbjct: 181 DLEKQKFYRFIHKTYWLHPLALSLLLYALGGLPFIIWGVCVRFVLSLHFTSMINSVCHIW 240

Query: 241 GNQPWNTGDLSKNNWIVAIFTFGEGWHNNHHAFEYSARHGHEWWQIDNGWYIIKFLQVIG 300
           GNQPWNTGDLSKNNWIVAIFTFGEGWHNNHHAFEYSARHGHEWWQIDNGWYIIKFLQVIG
Sbjct: 241 GNQPWNTGDLSKNNWIVAIFTFGEGWHNNHHAFEYSARHGHEWWQIDNGWYIIKFLQVIG 300

Query: 301 LATNVKLPSEKQKMAALNKSKPTFLRPMID 331
           LATNVKLPSEKQKMAALNKSKPTFLRPMID
Sbjct: 301 LATNVKLPSEKQKMAALNKSKPTFLRPMID 330

BLAST of CSPI07G15680.1 vs. NCBI nr
Match: gi|659100771|ref|XP_008451258.1| (PREDICTED: palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Cucumis melo])

HSP 1 Score: 657.5 bits (1695), Expect = 1.2e-185
Identity = 308/330 (93.33%), Postives = 316/330 (95.76%), Query Frame = 1

Query: 1   MEGASKEDPKVMVMSSPSRLFKRKKWTKFDKNVAFYFLLLHLLSILAPLFYFSWGCLLLS 60
           ME ASKE+ K MVM+SPSRLFKRKKWTKFDKNVAFYFLLLHLL ILAP FYFSW CL LS
Sbjct: 1   MEEASKEESKEMVMNSPSRLFKRKKWTKFDKNVAFYFLLLHLLCILAP-FYFSWTCLFLS 60

Query: 61  LGLANLTGMAISVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFV 120
           LGLANLTGM ISVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFV
Sbjct: 61  LGLANLTGMGISVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFV 120

Query: 121 DSERDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNILMFYMKHGYPDNVT 180
           DSERDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNN+QKVERN  MFYMKHGY DNVT
Sbjct: 121 DSERDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNFQKVERNAFMFYMKHGYADNVT 180

Query: 181 DLEKQKFYRFIHKTYWLHPLALSLLLYALGGLPFIIWGVCVRFVLSLHFTSMINSVCHIW 240
           DLEKQ+FYRFIHKTYWLHPLALSLLLYA+GGLPFIIWG+CVRFV+SLHFTSMINSVCHIW
Sbjct: 181 DLEKQRFYRFIHKTYWLHPLALSLLLYAIGGLPFIIWGMCVRFVISLHFTSMINSVCHIW 240

Query: 241 GNQPWNTGDLSKNNWIVAIFTFGEGWHNNHHAFEYSARHGHEWWQIDNGWYIIKFLQVIG 300
           GNQPWNTGDLSKNNWIVAIFTFGEGWHNNHHAFEYSARHGHEWWQID GWYIIKFLQVIG
Sbjct: 241 GNQPWNTGDLSKNNWIVAIFTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYIIKFLQVIG 300

Query: 301 LATNVKLPSEKQKMAALNKSKPTFLRPMID 331
            ATNVKLPSEKQK AALNKSKPTFL+PMID
Sbjct: 301 FATNVKLPSEKQKWAALNKSKPTFLKPMID 329

BLAST of CSPI07G15680.1 vs. NCBI nr
Match: gi|778727442|ref|XP_011659261.1| (PREDICTED: palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Cucumis sativus])

HSP 1 Score: 636.0 bits (1639), Expect = 3.8e-179
Identity = 295/330 (89.39%), Postives = 309/330 (93.64%), Query Frame = 1

Query: 1   MEGASKEDPKVMVMSSPSRLFKRKKWTKFDKNVAFYFLLLHLLSILAPLFYFSWGCLLLS 60
           M+  SKE PK MVM SP RLF+RKKWTKFD+NVAFYFL LHLL ILAP FYFSW CLLL+
Sbjct: 1   MDEVSKEKPKEMVMKSPPRLFRRKKWTKFDRNVAFYFLSLHLLCILAP-FYFSWTCLLLA 60

Query: 61  LGLANLTGMAISVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFV 120
           LGL+ LTGM ISVSYHRNLAHRSFKLPKWLEYSLAYIA HAIQGDPIDWVSTHRCHHRFV
Sbjct: 61  LGLSYLTGMGISVSYHRNLAHRSFKLPKWLEYSLAYIATHAIQGDPIDWVSTHRCHHRFV 120

Query: 121 DSERDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNILMFYMKHGYPDNVT 180
           DS RDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNI MFYMKHGYPDNVT
Sbjct: 121 DSARDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNIFMFYMKHGYPDNVT 180

Query: 181 DLEKQKFYRFIHKTYWLHPLALSLLLYALGGLPFIIWGVCVRFVLSLHFTSMINSVCHIW 240
           DLEKQKFYRFIHKTYWLHPLALSLLLYA+GGLPFIIWG+CVRFV SLH TSM+NSVCHIW
Sbjct: 181 DLEKQKFYRFIHKTYWLHPLALSLLLYAIGGLPFIIWGMCVRFVYSLHVTSMVNSVCHIW 240

Query: 241 GNQPWNTGDLSKNNWIVAIFTFGEGWHNNHHAFEYSARHGHEWWQIDNGWYIIKFLQVIG 300
           GNQPWNTGDLSKNNW+VA+ TFGEGWHNNHHAFEYSARHGHEWWQID GWY+I+FLQVIG
Sbjct: 241 GNQPWNTGDLSKNNWMVALLTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYLIRFLQVIG 300

Query: 301 LATNVKLPSEKQKMAALNKSKPTFLRPMID 331
           LATNVKLPSEKQK+AALNKSKP FL+ MID
Sbjct: 301 LATNVKLPSEKQKLAALNKSKPIFLKLMID 329

BLAST of CSPI07G15680.1 vs. NCBI nr
Match: gi|659100773|ref|XP_008451259.1| (PREDICTED: palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Cucumis melo])

HSP 1 Score: 597.0 bits (1538), Expect = 1.9e-167
Identity = 277/327 (84.71%), Postives = 298/327 (91.13%), Query Frame = 1

Query: 5   SKEDPK-VMVMSSPSRLFKRKKWTKFDKNVAFYFLLLHLLSILAPLFYFSWGCLLLSLGL 64
           SKE PK +++M SP +LF+RKKWTKFD+N AFYFL  HLL ILAP F FSW  LLL+LGL
Sbjct: 6   SKEKPKEIILMKSPPKLFRRKKWTKFDRNFAFYFLTFHLLCILAP-FCFSWTRLLLALGL 65

Query: 65  ANLTGMAISVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVDSE 124
           + L+GM ISVSYHRNLAHRSFKLPKW EYSLAYIAAHAIQGDPIDWVSTHRCHHRFVDSE
Sbjct: 66  SCLSGMGISVSYHRNLAHRSFKLPKWFEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVDSE 125

Query: 125 RDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNILMFYMKHGYPDNVTDLE 184
           RDPHSPIYGFWFSQMTW FDSYNLTRKVCPNYFNNYQK ERNI M YMKHGYPDNV DLE
Sbjct: 126 RDPHSPIYGFWFSQMTWLFDSYNLTRKVCPNYFNNYQKAERNIFMLYMKHGYPDNVPDLE 185

Query: 185 KQKFYRFIHKTYWLHPLALSLLLYALGGLPFIIWGVCVRFVLSLHFTSMINSVCHIWGNQ 244
           KQ FYRFIHKTYWLHPLALSLLLYA+GGLPFIIWG+CVRFV S+H TSM+NSVCH+WGNQ
Sbjct: 186 KQWFYRFIHKTYWLHPLALSLLLYAIGGLPFIIWGMCVRFVYSVHVTSMVNSVCHMWGNQ 245

Query: 245 PWNTGDLSKNNWIVAIFTFGEGWHNNHHAFEYSARHGHEWWQIDNGWYIIKFLQVIGLAT 304
           PWNTGDLSKNNW+VA+ TFGEGWHNNHHAFEYSARHG EWWQID GWY I+FL+VIGLAT
Sbjct: 246 PWNTGDLSKNNWMVALLTFGEGWHNNHHAFEYSARHGLEWWQIDYGWYFIRFLEVIGLAT 305

Query: 305 NVKLPSEKQKMAALNKSKPTFLRPMID 331
           NVK PSEKQK+AALNKSKPTFL+P I+
Sbjct: 306 NVKFPSEKQKLAALNKSKPTFLKPTIN 331

BLAST of CSPI07G15680.1 vs. NCBI nr
Match: gi|700189514|gb|KGN44747.1| (hypothetical protein Csa_7G377870 [Cucumis sativus])

HSP 1 Score: 570.9 bits (1470), Expect = 1.5e-159
Identity = 261/262 (99.62%), Postives = 261/262 (99.62%), Query Frame = 1

Query: 69  MAISVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVDSERDPHS 128
           MAISVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVDSERDPHS
Sbjct: 1   MAISVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVDSERDPHS 60

Query: 129 PIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNILMFYMKHGYPDNVTDLEKQKFY 188
           PIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNI MFYMKHGYPDNVTDLEKQKFY
Sbjct: 61  PIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNIFMFYMKHGYPDNVTDLEKQKFY 120

Query: 189 RFIHKTYWLHPLALSLLLYALGGLPFIIWGVCVRFVLSLHFTSMINSVCHIWGNQPWNTG 248
           RFIHKTYWLHPLALSLLLYALGGLPFIIWGVCVRFVLSLHFTSMINSVCHIWGNQPWNTG
Sbjct: 121 RFIHKTYWLHPLALSLLLYALGGLPFIIWGVCVRFVLSLHFTSMINSVCHIWGNQPWNTG 180

Query: 249 DLSKNNWIVAIFTFGEGWHNNHHAFEYSARHGHEWWQIDNGWYIIKFLQVIGLATNVKLP 308
           DLSKNNWIVAIFTFGEGWHNNHHAFEYSARHGHEWWQIDNGWYIIKFLQVIGLATNVKLP
Sbjct: 181 DLSKNNWIVAIFTFGEGWHNNHHAFEYSARHGHEWWQIDNGWYIIKFLQVIGLATNVKLP 240

Query: 309 SEKQKMAALNKSKPTFLRPMID 331
           SEKQKMAALNKSKPTFLRPMID
Sbjct: 241 SEKQKMAALNKSKPTFLRPMID 262

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
ADS3_ARATH3.3e-9855.59Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic OS=Arab... [more]
ADS32_ARATH1.2e-8750.83Probable lipid desaturase ADS3.2, chloroplastic OS=Arabidopsis thaliana GN=ADS3.... [more]
ADS1_ARATH7.1e-8550.33Delta-9 acyl-lipid desaturase 1 OS=Arabidopsis thaliana GN=ADS1 PE=2 SV=1[more]
ADS2_ARATH1.2e-8451.36Delta-9 acyl-lipid desaturase 2 OS=Arabidopsis thaliana GN=ADS2 PE=2 SV=2[more]
ICO5D_LIMDO1.1e-8250.16Icosanoyl-CoA 5-desaturase (Fragment) OS=Limnanthes douglasii PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KAB1_CUCSA2.6e-17989.39Uncharacterized protein OS=Cucumis sativus GN=Csa_7G376370 PE=3 SV=1[more]
A0A0A0K8P8_CUCSA1.0e-15999.62Uncharacterized protein OS=Cucumis sativus GN=Csa_7G377870 PE=3 SV=1[more]
A0A0A0KVH2_CUCSA2.3e-12262.54Uncharacterized protein OS=Cucumis sativus GN=Csa_4G006060 PE=3 SV=1[more]
A0A0A0KYH4_CUCSA1.2e-12065.13Uncharacterized protein OS=Cucumis sativus GN=Csa_4G006130 PE=3 SV=1[more]
A0A0A0LTL5_CUCSA1.3e-10960.06Uncharacterized protein OS=Cucumis sativus GN=Csa_1G227450 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G15850.11.8e-9955.59 fatty acid desaturase 5[more]
AT3G15870.16.6e-8950.83 Fatty acid desaturase family protein[more]
AT1G06080.14.0e-8650.33 delta 9 desaturase 1[more]
AT2G31360.16.8e-8651.36 16:0delta9 desaturase 2[more]
AT1G06100.12.7e-8251.53 Fatty acid desaturase family protein[more]
Match NameE-valueIdentityDescription
gi|778730379|ref|XP_011659770.1|4.8e-19899.39PREDICTED: palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplas... [more]
gi|659100771|ref|XP_008451258.1|1.2e-18593.33PREDICTED: palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplas... [more]
gi|778727442|ref|XP_011659261.1|3.8e-17989.39PREDICTED: palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplas... [more]
gi|659100773|ref|XP_008451259.1|1.9e-16784.71PREDICTED: palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplas... [more]
gi|700189514|gb|KGN44747.1|1.5e-15999.62hypothetical protein Csa_7G377870 [Cucumis sativus][more]
The following terms have been associated with this mRNA:
Vocabulary: INTERPRO
TermDefinition
IPR005804FA_desaturase_dom
IPR015876Acyl-CoA_DS
Vocabulary: Biological Process
TermDefinition
GO:0006629lipid metabolic process
GO:0055114oxidation-reduction process
Vocabulary: Molecular Function
TermDefinition
GO:0016717oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006629 lipid metabolic process
biological_process GO:0055114 oxidation-reduction process
biological_process GO:0006633 fatty acid biosynthetic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0016717 oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
CSPI07G15680CSPI07G15680gene


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
CSPI07G15680.1CSPI07G15680.1-proteinpolypeptide


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
CSPI07G15680.1.cds4CSPI07G15680.1.cds4CDS
CSPI07G15680.1.cds3CSPI07G15680.1.cds3CDS
CSPI07G15680.1.cds2CSPI07G15680.1.cds2CDS
CSPI07G15680.1.cds1CSPI07G15680.1.cds1CDS


Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005804Fatty acid desaturase domainPFAMPF00487FA_desaturasecoord: 54..290
score: 2.1
IPR015876Fatty acid desaturase, type 1, corePRINTSPR00075FACDDSATRASEcoord: 113..142
score: 3.1E-9coord: 76..96
score: 3.
NoneNo IPR availablePANTHERPTHR11351ACYL-COA DESATURASEcoord: 12..330
score: 5.7E
NoneNo IPR availablePANTHERPTHR11351:SF2DELTA-9 ACYL-LIPID DESATURASE 1-RELATEDcoord: 12..330
score: 5.7E