CSPI07G15670.1 (mRNA) Wild cucumber (PI 183967)

NameCSPI07G15670.1
TypemRNA
OrganismCucumis sativus (Wild cucumber (PI 183967))
DescriptionDelta-9 fatty acid desaturase
LocationChr7 : 14283740 .. 14286506 (-)
Sequence length990
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTCACCACCAGCTATACTATACGAAAGAAAGAATAAGAGGAAAGAAATGGATGAAGTATCCAAAGAAAAGCCAAAGGAGATGGTGATGAAATCTCCTCCGAGGCTCTTTAGGAGAAAAAAATGGACAAAGTTTGATAGAAACGTTGCCTTTTACTTTCTCAGTTTGCATCTACTTTGTATTTTGGCACCCTTCTATTTTAGTTGGACTTGTTTGTTGCTCGCCTTAGGCTTATCTTATTTAACAGGTATGGGAATAAGTGTCTCGTATCATCGAAATCTTGCACATCGAAGCTTCAAGCTTCCAAAATGGCTCGAGTATTCTCTTGCTTATATTGCAACTCATGCAATCCAGGTTTGATATTTTGAATTCTTTGATTTTATATTTAGTTTAAGACTATACATGATGGATAATACAAAATATTTAAATTTTTTGTCACACATTAGTATAAACTTATAAATCAATAAGATATGTAACATAATATATATGGGAGTAGGTGGTTCATTATTGATTTTTACATATGGTAACCCTACAAGTGAGGATTTATTAGTGTTGCCTTAGATACTTTGGATTCATTTGTCATGCCAAAATCATCATTATTTATGGTTATGCCATATTTTGTGATAATTTGTATATACACTACTTAAAACACCAAAACATTATTAAAGAATGGTGTAGTGTAATTATTGCCTACATGTGATTTAGAAGGGTAAAACTAGTGACAAAACCCTAGTGCATATTTTTTTATATCTACCGTGTTTTGTTAAAATTTCGTTTACTTTTTTCTTTCTTAATTTTAGGGTGATCCAATTGATTGGGTGAGTACACATAGATGTCATCATCGATTCGTTGATTCAGCACGAGATCCACATAGTCCTATTTATGGATTCTGGTTTAGTCAAATGACTTGGTTTTTTGATTCGTATAACTTGACGAGAAAAGTTTGTCCAAACTATTTTAATAATTATCAAAAGGTGGAGAGAAACATTGCCATGTTTTACATGAAACATGGATATCCAGACAATGTGACAGATTTGGAGAAACAAAGGTTCTATAGATTTCTACATAAAACATATTGGCTTCATCCACTTGCCCTTTCACTCTTGCTCTATGCCATTGGAGGACTACCTTTCATCATTTGGGGAATGGTATAATTATTCCCATTCTTTTTTAGCTTTTACTCCTACAATTTGTAAGTTGTGATTAAATTTATTTATGAGTCCTCTAAAAATTAGTTTACATTCATCTTTTATAAATTCTATTTGTTTGGATTTTTGACATTTTAACTTAACTTTGGTAAACTAGCAAATTTAATAATTCTTTTTGAAGGAAAGATTGTATGTTTTTGTATTGACATAACAAAGGTAGTTAATTTTCTTTTTGACTATTTTACTTAAATGTTTACTCAAATATTATTGTTTATCTTTTATGAGCTGTGACAGACCAAAATAAACTATTATTTAAGTTCTATTGTGATAGACACATGTAATAGTCTATATTTTGATCCGTCATACCTAAATTGTGCTATTTTAATATATTTGTAGTAGTTTTGTCTTTTTTATTATTGTTTTATTTTACAATTAATCATTGATTAACATTATTTATTTGGTTAAACTATAAAATTTAATTAGTCTTCAACGTTTGAAACTTGTTCATGTCTAATTAAGTTTAAGTCTTTATAAAAGATCTAGAAGTTAAAAGTTATACTGTGTATTTAGTTAAATTCAATCTTTTATTTAAGAAGTTTGTGAATTTTAGGCTTCTTTGATAATAATTAATTTGTTATTTTTTTCTTCTCTTTTTTGTGTAAAACTTATATATACTTTTTTTCTCCCCATTTTTAATCTATGGTTTCAAATTGTTTGCAAATAATAATATTTTTAGTCAATTCCAATCAACAACCAAAATTTGTAAGATATATGTATTTTTTTATTTTACTTTTCAAAACTTGATCACTGTTTAATATGATTAGCCATAAATATAAGTAGAAAAGGTAGTTGAAATGTATTTTCTAAATGATGTAAGTTCCTTTTCTTCATAAATAGTTTTACCATCTAGTTAATTATTCTAGATTTAATGATTATAAAATAATAATTATAATTTTATTAATTATTTTCATTGAAATATTTTACTAAACTAAGTGTTTTGTGTTTATTAAACTTGCGTATATAGTGCGTGAGGTTCGTATACAGCTTGCATGTGACATCTATGGTCAATTCAGTATGTCATATATGGGGAAATCAACCATGGAATACTGGTGATTTATCCAAGAATAATTGGTATGACATTTATTTTATTTTACTTTCCTTTGAATCCCTCAAAATTCAATTTTACTCCCTACATTTTCAAAATACTTTTCACAACCCTAATTTAGATAAAATCATATTTGAATAATATATGTTCTCTTTTCGTTGTTCTTTTCTTCTTTTATAGGATGGTAGCTTTATTAACCTTTGGAGAAGGATGGCACAATAACCACCATGCTTTTGAGTATTCAGCGAGACATGGTCATGAGTGGTGGCAAATTGATTATGGTTGGTATCTTATTAGGTTCCTTCAGGTCATTGGATTAGCAACCAATGTGAAATTACCTTCTGAAAAGCAAAAATTGGCTGCTTTAAACAAATCAAAACCCATCTTTCTTAAGCTTATGATTGATTGAGATTAATCATATTTAATCTTTATATATATATATATATGCATGAATGCTTATGTACTCTATCATGGTAGGGTTTACGTAACCCTTCTCTCAATTATATGTATCCTTAC

mRNA sequence

ATGGATGAAGTATCCAAAGAAAAGCCAAAGGAGATGGTGATGAAATCTCCTCCGAGGCTCTTTAGGAGAAAAAAATGGACAAAGTTTGATAGAAACGTTGCCTTTTACTTTCTCAGTTTGCATCTACTTTGTATTTTGGCACCCTTCTATTTTAGTTGGACTTGTTTGTTGCTCGCCTTAGGCTTATCTTATTTAACAGGTATGGGAATAAGTGTCTCGTATCATCGAAATCTTGCACATCGAAGCTTCAAGCTTCCAAAATGGCTCGAGTATTCTCTTGCTTATATTGCAACTCATGCAATCCAGGGTGATCCAATTGATTGGGTGAGTACACATAGATGTCATCATCGATTCGTTGATTCAGCACGAGATCCACATAGTCCTATTTATGGATTCTGGTTTAGTCAAATGACTTGGTTTTTTGATTCGTATAACTTGACGAGAAAAGTTTGTCCAAACTATTTTAATAATTATCAAAAGGTGGAGAGAAACATTGCCATGTTTTACATGAAACATGGATATCCAGACAATGTGACAGATTTGGAGAAACAAAGGTTCTATAGATTTCTACATAAAACATATTGGCTTCATCCACTTGCCCTTTCACTCTTGCTCTATGCCATTGGAGGACTACCTTTCATCATTTGGGGAATGTGCGTGAGGTTCGTATACAGCTTGCATGTGACATCTATGGTCAATTCAGTATGTCATATATGGGGAAATCAACCATGGAATACTGGTGATTTATCCAAGAATAATTGGATGGTAGCTTTATTAACCTTTGGAGAAGGATGGCACAATAACCACCATGCTTTTGAGTATTCAGCGAGACATGGTCATGAGTGGTGGCAAATTGATTATGGTTGGTATCTTATTAGGTTCCTTCAGGTCATTGGATTAGCAACCAATGTGAAATTACCTTCTGAAAAGCAAAAATTGGCTGCTTTAAACAAATCAAAACCCATCTTTCTTAAGCTTATGATTGATTGA

Coding sequence (CDS)

ATGGATGAAGTATCCAAAGAAAAGCCAAAGGAGATGGTGATGAAATCTCCTCCGAGGCTCTTTAGGAGAAAAAAATGGACAAAGTTTGATAGAAACGTTGCCTTTTACTTTCTCAGTTTGCATCTACTTTGTATTTTGGCACCCTTCTATTTTAGTTGGACTTGTTTGTTGCTCGCCTTAGGCTTATCTTATTTAACAGGTATGGGAATAAGTGTCTCGTATCATCGAAATCTTGCACATCGAAGCTTCAAGCTTCCAAAATGGCTCGAGTATTCTCTTGCTTATATTGCAACTCATGCAATCCAGGGTGATCCAATTGATTGGGTGAGTACACATAGATGTCATCATCGATTCGTTGATTCAGCACGAGATCCACATAGTCCTATTTATGGATTCTGGTTTAGTCAAATGACTTGGTTTTTTGATTCGTATAACTTGACGAGAAAAGTTTGTCCAAACTATTTTAATAATTATCAAAAGGTGGAGAGAAACATTGCCATGTTTTACATGAAACATGGATATCCAGACAATGTGACAGATTTGGAGAAACAAAGGTTCTATAGATTTCTACATAAAACATATTGGCTTCATCCACTTGCCCTTTCACTCTTGCTCTATGCCATTGGAGGACTACCTTTCATCATTTGGGGAATGTGCGTGAGGTTCGTATACAGCTTGCATGTGACATCTATGGTCAATTCAGTATGTCATATATGGGGAAATCAACCATGGAATACTGGTGATTTATCCAAGAATAATTGGATGGTAGCTTTATTAACCTTTGGAGAAGGATGGCACAATAACCACCATGCTTTTGAGTATTCAGCGAGACATGGTCATGAGTGGTGGCAAATTGATTATGGTTGGTATCTTATTAGGTTCCTTCAGGTCATTGGATTAGCAACCAATGTGAAATTACCTTCTGAAAAGCAAAAATTGGCTGCTTTAAACAAATCAAAACCCATCTTTCTTAAGCTTATGATTGATTGA
BLAST of CSPI07G15670.1 vs. Swiss-Prot
Match: ADS3_ARATH (Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic OS=Arabidopsis thaliana GN=ADS3 PE=1 SV=2)

HSP 1 Score: 371.7 bits (953), Expect = 8.3e-102
Identity = 173/302 (57.28%), Postives = 214/302 (70.86%), Query Frame = 1

Query: 12  MVMKSPPRLFRRKKWTKFDRNVAFYFLSLHLLCILAPFYFSWTCLLLALGLSYLTGM-GI 71
           +V K    ++  ++W   D       LS+HLL +LAPF F+W  + +A GL  +TG+ GI
Sbjct: 84  LVKKKEKVVWWEREWKAMDFGAVAVVLSMHLLSLLAPFQFNWRAVSVAFGLYIVTGLLGI 143

Query: 72  SVSYHRNLAHRSFKLPKWLEYSLAYIATHAIQGDPIDWVSTHRCHHRFVDSARDPHSPIY 131
           ++S+HRNL+H++FKLPKWLEY  AY    A+QG+PIDWVSTHR HH+F DS RDPHSP+ 
Sbjct: 144 TLSFHRNLSHKAFKLPKWLEYLFAYCGAQALQGNPIDWVSTHRYHHQFCDSDRDPHSPLD 203

Query: 132 GFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNIAMFYMKHGYPDNVTDLEKQRFYRFL 191
           GFWFS M W FD+  +T++                       G P+NV DLEKQ FYRFL
Sbjct: 204 GFWFSHMNWMFDTNTITQRC----------------------GEPNNVGDLEKQPFYRFL 263

Query: 192 HKTYWLHPLALSLLLYAIGGLPFIIWGMCVRFVYSLHVTSMVNSVCHIWGNQPWNTGDLS 251
             TY LHPLAL++ LYA+GG PFI+WGM VR V+  H+T +VNS CH+WG Q WNTGDLS
Sbjct: 264 RTTYILHPLALAVALYAMGGFPFIVWGMGVRIVWVYHITWLVNSACHVWGKQAWNTGDLS 323

Query: 252 KNNWMVALLTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYLIRFLQVIGLATNVKLPSEK 311
           KNNW VA L FGEGWHNNHHAFE+SARHG EWWQ+D  WY+++FLQ IGLAT+VKLPSE 
Sbjct: 324 KNNWWVAALAFGEGWHNNHHAFEFSARHGLEWWQLDMTWYVVKFLQAIGLATDVKLPSEA 363

Query: 312 QK 313
           QK
Sbjct: 384 QK 363

BLAST of CSPI07G15670.1 vs. Swiss-Prot
Match: ADS32_ARATH (Probable lipid desaturase ADS3.2, chloroplastic OS=Arabidopsis thaliana GN=ADS3.2 PE=2 SV=3)

HSP 1 Score: 333.6 bits (854), Expect = 2.5e-90
Identity = 157/301 (52.16%), Postives = 195/301 (64.78%), Query Frame = 1

Query: 11  EMVMKSPPRLFRRKKWTKFDRNVAFYFLSLHLLCILAPFYFSWTCLLLALGLSYLTGMGI 70
           E+V+    R F  + WT +D +    F+  HLL +LAPFYFSW    +   L ++ G+ I
Sbjct: 76  EVVVVRTKRSFWERSWTSWDVSKLVIFVGTHLLSLLAPFYFSWEAFWVFPWLVFINGICI 135

Query: 71  SVSYHRNLAHRSFKLPKWLEYSLAYIATHAIQGDPIDWVSTHRCHHRFVDSARDPHSPIY 130
           ++SYHRNL+HRSF LPKWLEY  AY    A QGDPI+WVS HR HH+  ++ RDPHSP  
Sbjct: 136 TLSYHRNLSHRSFDLPKWLEYLFAYGGVLAFQGDPIEWVSNHRYHHKHCETQRDPHSPTQ 195

Query: 131 GFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNIAMFYMKHGYPDNVTDLEKQRFYRFL 190
           GFWFS M W FD+ ++                          G  +NV DL +Q FYRFL
Sbjct: 196 GFWFSHMAWIFDTSSILENC----------------------GGEENVDDLVRQPFYRFL 255

Query: 191 HKTYWLHPLALSLLLYAIGGLPFIIWGMCVRFVYSLHVTSMVNSVCHIWGNQPWNTGDLS 250
            +T  LH +A S L Y  GG+P ++WG+ +     LH+T +VNSVCHIWG + WNT D S
Sbjct: 256 QRTVLLHMMAYSFLFYFCGGMPLLVWGIGITIAVRLHLTFLVNSVCHIWGTRAWNTSDFS 315

Query: 251 KNNWMVALLTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYLIRFLQVIGLATNVKLPSEK 310
           KNNW VA+LT GEGWHNNHHAFE+SARHG EWWQ+D  W LIRFL+ IGLATNVKLP+E 
Sbjct: 316 KNNWWVAILTLGEGWHNNHHAFEFSARHGLEWWQLDITWCLIRFLEAIGLATNVKLPTET 354

Query: 311 Q 312
           Q
Sbjct: 376 Q 354

BLAST of CSPI07G15670.1 vs. Swiss-Prot
Match: ADS1_ARATH (Delta-9 acyl-lipid desaturase 1 OS=Arabidopsis thaliana GN=ADS1 PE=2 SV=1)

HSP 1 Score: 325.5 bits (833), Expect = 6.8e-88
Identity = 154/294 (52.38%), Postives = 192/294 (65.31%), Query Frame = 1

Query: 19  RLFRRKKWTKFDRNVAFYFLSLHLLCILAPFYFSWTCLLLALGLSYLTGMGISVSYHRNL 78
           R    +KW + D   AF  L +H LC+LAPF F+W  L +AL +  + G+GI+VSYHRNL
Sbjct: 27  RAMWERKWKRLDIVKAFASLFVHFLCLLAPFNFTWPALRVALIVYTVGGLGITVSYHRNL 86

Query: 79  AHRSFKLPKWLEYSLAYIATHAIQGDPIDWVSTHRCHHRFVDSARDPHSPIYGFWFSQMT 138
           AHRSFK+PKWLEY  AY    AIQGDPIDWVSTHR HH+F DS RDPHSP  GFWFS + 
Sbjct: 87  AHRSFKVPKWLEYFFAYCGLLAIQGDPIDWVSTHRYHHQFTDSDRDPHSPNEGFWFSHLL 146

Query: 139 WFFDSYNLTRKVCPNYFNNYQKVERNIAMFYMKHGYPDNVTDLEKQRFYRFLHKTYWLHP 198
           W FD+  L  K                       G   NV DL++Q +Y+FL +T   H 
Sbjct: 147 WLFDTGYLVEKC----------------------GRRTNVEDLKRQWYYKFLQRTVLYHI 206

Query: 199 LALSLLLYAIGGLPFIIWGMCVRFVYSLHVTSMVNSVCHIWGNQPWNTGDLSKNNWMVAL 258
           L    LLY  GGL F+ WGM +      HVT ++NS+CH+WG++ W T D S+N W +++
Sbjct: 207 LTFGFLLYYFGGLSFLTWGMGIGVAMEHHVTCLINSLCHVWGSRTWKTNDTSRNVWWLSV 266

Query: 259 LTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYLIRFLQVIGLATNVKLPSEKQK 313
            +FGE WHNNHHAFE SAR G EWWQID  WY++RFL++IGLAT+VKLPSE Q+
Sbjct: 267 FSFGESWHNNHHAFESSARQGLEWWQIDISWYIVRFLEIIGLATDVKLPSESQR 298

BLAST of CSPI07G15670.1 vs. Swiss-Prot
Match: ADS2_ARATH (Delta-9 acyl-lipid desaturase 2 OS=Arabidopsis thaliana GN=ADS2 PE=2 SV=2)

HSP 1 Score: 319.7 bits (818), Expect = 3.7e-86
Identity = 150/293 (51.19%), Postives = 191/293 (65.19%), Query Frame = 1

Query: 20  LFRRKKWTKFDRNVAFYFLSLHLLCILAPFYFSWTCLLLALGLSYLTGMGISVSYHRNLA 79
           +F  ++W + D        ++H L +LAPFYF+W+ L +      + G+GI+VSYHRNLA
Sbjct: 30  VFWDRRWRRLDYVKFSASFTVHSLALLAPFYFTWSALWVTFLFYTIGGLGITVSYHRNLA 89

Query: 80  HRSFKLPKWLEYSLAYIATHAIQGDPIDWVSTHRCHHRFVDSARDPHSPIYGFWFSQMTW 139
           HRSFK+PKWLEY LAY A  AIQGDPIDWVSTHR HH+F DS RDPHSP  GFWFS + W
Sbjct: 90  HRSFKVPKWLEYLLAYCALLAIQGDPIDWVSTHRYHHQFTDSERDPHSPKEGFWFSHLLW 149

Query: 140 FFDSYNLTRKVCPNYFNNYQKVERNIAMFYMKHGYPDNVTDLEKQRFYRFLHKTYWLHPL 199
            +DS  L  K                       G   NV DL++Q FYRFL KT   H L
Sbjct: 150 IYDSAYLVSKC----------------------GRRANVEDLKRQWFYRFLQKTVLFHIL 209

Query: 200 ALSLLLYAIGGLPFIIWGMCVRFVYSLHVTSMVNSVCHIWGNQPWNTGDLSKNNWMVALL 259
            L   L+ +GG+ F+ WGM V     +HVT ++NS+CHIWG + W T D S+N W +++ 
Sbjct: 210 GLGFFLFYLGGMSFVTWGMGVGAALEVHVTCLINSLCHIWGTRTWKTNDTSRNVWWLSVF 269

Query: 260 TFGEGWHNNHHAFEYSARHGHEWWQIDYGWYLIRFLQVIGLATNVKLPSEKQK 313
           +FGE WHNNHHAFE SAR G EWWQID  WY++RF ++IGLAT+VK+P+E Q+
Sbjct: 270 SFGESWHNNHHAFESSARQGLEWWQIDISWYIVRFFEIIGLATDVKVPTEAQR 300

BLAST of CSPI07G15670.1 vs. Swiss-Prot
Match: ICO5D_LIMDO (Icosanoyl-CoA 5-desaturase (Fragment) OS=Limnanthes douglasii PE=1 SV=1)

HSP 1 Score: 318.9 bits (816), Expect = 6.4e-86
Identity = 161/320 (50.31%), Postives = 200/320 (62.50%), Query Frame = 1

Query: 2   DEVSKEKPKEMVMKSPPRLFRRKKWTKFDRNVAFYFLSLHLLCILAPFYFSWTCLLLA-L 61
           D++ + +  E+V++   + F R+KW   D   A   L LH L  +APFYFSW    ++ +
Sbjct: 57  DDLERFRTSEVVLERKAKGFWRRKWNPRDIQNAVTLLVLHALAAMAPFYFSWDAFWISFI 116

Query: 62  GLSYLTG-MGISVSYHRNLAHRSFKLPKWLEYSLAYIATHAIQGDPIDWVSTHRCHHRFV 121
            L + +G +GI++ +HR L H  FKLPK +EY  AY  + A+QGDP++WVS HR HH+FV
Sbjct: 117 LLGFASGVLGITLCFHRCLTHGGFKLPKLVEYFFAYCGSLALQGDPMEWVSNHRYHHQFV 176

Query: 122 DSARDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNIAMFYMKHGYPDNVT 181
           D+ RD HSP  GFWF  + W  D      K                     + G  +NV 
Sbjct: 177 DTERDVHSPTQGFWFCHIGWVLDKDLFVEK---------------------RGGRRNNVN 236

Query: 182 DLEKQRFYRFLHKTYWLHPLALSLLLYAIGGLPFIIWGMCVRFVYSLHVTSMVNSVCHIW 241
           DL+KQ FYRFL KTY  H LAL  LLY +GG P+I+WGM  R V+  H T  +NSVCH W
Sbjct: 237 DLKKQAFYRFLQKTYMYHQLALIALLYYVGGFPYIVWGMGFRLVFMFHSTFAINSVCHKW 296

Query: 242 GNQPWNTGDLSKNNWMVALLTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYLIRFLQVIG 301
           G +PWNTGDLS NN  VAL  FGEGWHNNHHAFE SARHG EWW+ID  WY+IR LQ IG
Sbjct: 297 GGRPWNTGDLSTNNMFVALCAFGEGWHNNHHAFEQSARHGLEWWEIDVTWYVIRTLQAIG 355

Query: 302 LATNVKLPSEKQKLAALNKS 320
           LATNVKLP+E QK     KS
Sbjct: 357 LATNVKLPTEAQKQKLKAKS 355

BLAST of CSPI07G15670.1 vs. TrEMBL
Match: A0A0A0KAB1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G376370 PE=3 SV=1)

HSP 1 Score: 698.7 bits (1802), Expect = 3.3e-198
Identity = 326/329 (99.09%), Postives = 328/329 (99.70%), Query Frame = 1

Query: 1   MDEVSKEKPKEMVMKSPPRLFRRKKWTKFDRNVAFYFLSLHLLCILAPFYFSWTCLLLAL 60
           MDEVSKEKPKEMVMKSPPRLFRRKKWTKFDRNVAFYFLSLHLLCILAPFYFSWTCLLLAL
Sbjct: 1   MDEVSKEKPKEMVMKSPPRLFRRKKWTKFDRNVAFYFLSLHLLCILAPFYFSWTCLLLAL 60

Query: 61  GLSYLTGMGISVSYHRNLAHRSFKLPKWLEYSLAYIATHAIQGDPIDWVSTHRCHHRFVD 120
           GLSYLTGMGISVSYHRNLAHRSFKLPKWLEYSLAYIATHAIQGDPIDWVSTHRCHHRFVD
Sbjct: 61  GLSYLTGMGISVSYHRNLAHRSFKLPKWLEYSLAYIATHAIQGDPIDWVSTHRCHHRFVD 120

Query: 121 SARDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNIAMFYMKHGYPDNVTD 180
           SARDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNI MFYMKHGYPDNVTD
Sbjct: 121 SARDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNIFMFYMKHGYPDNVTD 180

Query: 181 LEKQRFYRFLHKTYWLHPLALSLLLYAIGGLPFIIWGMCVRFVYSLHVTSMVNSVCHIWG 240
           LEKQ+FYRF+HKTYWLHPLALSLLLYAIGGLPFIIWGMCVRFVYSLHVTSMVNSVCHIWG
Sbjct: 181 LEKQKFYRFIHKTYWLHPLALSLLLYAIGGLPFIIWGMCVRFVYSLHVTSMVNSVCHIWG 240

Query: 241 NQPWNTGDLSKNNWMVALLTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYLIRFLQVIGL 300
           NQPWNTGDLSKNNWMVALLTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYLIRFLQVIGL
Sbjct: 241 NQPWNTGDLSKNNWMVALLTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYLIRFLQVIGL 300

Query: 301 ATNVKLPSEKQKLAALNKSKPIFLKLMID 330
           ATNVKLPSEKQKLAALNKSKPIFLKLMID
Sbjct: 301 ATNVKLPSEKQKLAALNKSKPIFLKLMID 329

BLAST of CSPI07G15670.1 vs. TrEMBL
Match: A0A0A0K8P8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G377870 PE=3 SV=1)

HSP 1 Score: 538.5 bits (1386), Expect = 5.7e-150
Identity = 241/262 (91.98%), Postives = 252/262 (96.18%), Query Frame = 1

Query: 68  MGISVSYHRNLAHRSFKLPKWLEYSLAYIATHAIQGDPIDWVSTHRCHHRFVDSARDPHS 127
           M ISVSYHRNLAHRSFKLPKWLEYSLAYIA HAIQGDPIDWVSTHRCHHRFVDS RDPHS
Sbjct: 1   MAISVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVDSERDPHS 60

Query: 128 PIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNIAMFYMKHGYPDNVTDLEKQRFY 187
           PIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNI MFYMKHGYPDNVTDLEKQ+FY
Sbjct: 61  PIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNIFMFYMKHGYPDNVTDLEKQKFY 120

Query: 188 RFLHKTYWLHPLALSLLLYAIGGLPFIIWGMCVRFVYSLHVTSMVNSVCHIWGNQPWNTG 247
           RF+HKTYWLHPLALSLLLYA+GGLPFIIWG+CVRFV SLH TSM+NSVCHIWGNQPWNTG
Sbjct: 121 RFIHKTYWLHPLALSLLLYALGGLPFIIWGVCVRFVLSLHFTSMINSVCHIWGNQPWNTG 180

Query: 248 DLSKNNWMVALLTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYLIRFLQVIGLATNVKLP 307
           DLSKNNW+VA+ TFGEGWHNNHHAFEYSARHGHEWWQID GWY+I+FLQVIGLATNVKLP
Sbjct: 181 DLSKNNWIVAIFTFGEGWHNNHHAFEYSARHGHEWWQIDNGWYIIKFLQVIGLATNVKLP 240

Query: 308 SEKQKLAALNKSKPIFLKLMID 330
           SEKQK+AALNKSKP FL+ MID
Sbjct: 241 SEKQKMAALNKSKPTFLRPMID 262

BLAST of CSPI07G15670.1 vs. TrEMBL
Match: A0A0A0KVH2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G006060 PE=3 SV=1)

HSP 1 Score: 461.1 bits (1185), Expect = 1.2e-126
Identity = 211/323 (65.33%), Postives = 253/323 (78.33%), Query Frame = 1

Query: 1   MDEVSKEKPKEMVMKSPPRLFRRKKWTKFDRNVAFYFLSLHLLCILAPFYFSWTCLLLAL 60
           MDE  +E+PK+MV   PPR F R+ WT  D+ VA+  L +HLLCI APF F+W+   + +
Sbjct: 18  MDESKQEEPKKMV--KPPRSFWRRNWTTLDKRVAYIILFVHLLCIFAPFQFNWSAFRVTV 77

Query: 61  GLSYLTGM-GISVSYHRNLAHRSFKLPKWLEYSLAYIATHAIQGDPIDWVSTHRCHHRFV 120
            L  +TG+ G+++SYHRNLAH+SF+LPKWLEYS AY   H +QGDP+DWVSTHRCHH+FV
Sbjct: 78  ALIVITGLFGMTISYHRNLAHKSFQLPKWLEYSFAYCGVHCLQGDPLDWVSTHRCHHQFV 137

Query: 121 DSARDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNIAMFYMKHGYPDNVT 180
           DS +DPHSPI GFW S M W FDSY LT KVCP Y ++ +K+ERN+ + + KHG PDNV 
Sbjct: 138 DSEKDPHSPINGFWISHMMWLFDSYTLTNKVCPKYSSDLKKIERNMFVIFTKHGKPDNVG 197

Query: 181 DLEKQRFYRFLHKTYWLHPLALSLLLYAIGGLPFIIWGMCVRFVYSLHVTSMVNSVCHIW 240
           DLEKQRFYRF+HKTY LH LAL+++LY+IGG+PF IWGMCVR V   H T MVNSVCHIW
Sbjct: 198 DLEKQRFYRFIHKTYMLHHLALAIILYSIGGVPFFIWGMCVRIVAVFHSTFMVNSVCHIW 257

Query: 241 GNQPWNTGDLSKNNWMVALLTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYLIRFLQVIG 300
           G QPW TGDLS+NNW+V L +FGEGWHNNHHAF YSAR G EWWQ+D GWY+I+FLQVIG
Sbjct: 258 GKQPWRTGDLSRNNWLVGLTSFGEGWHNNHHAFSYSARLGLEWWQLDAGWYVIKFLQVIG 317

Query: 301 LATNVKLPS--EKQKLAALNKSK 321
           LATNVKLPS    Q LA  +K K
Sbjct: 318 LATNVKLPSPTHMQSLAMDHKPK 338

BLAST of CSPI07G15670.1 vs. TrEMBL
Match: A0A0A0KYH4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G006130 PE=3 SV=1)

HSP 1 Score: 454.1 bits (1167), Expect = 1.4e-124
Identity = 205/309 (66.34%), Postives = 249/309 (80.58%), Query Frame = 1

Query: 7   EKPKEMVMKSPPRLFRRKKWTKFDRNVAFYFLSLHLLCILAPFYFSWTCLLLALGLSYLT 66
           +KPK   M   PR F+R+K+TK D+ + F    +H+ CI APF+F+W    +A  L ++T
Sbjct: 5   QKPK--AMGKSPRSFKRRKYTKLDKIIFFSLFIVHIACIFAPFHFTWPAFCVAFALYFIT 64

Query: 67  GMGISVSYHRNLAHRSFKLPKWLEYSLAYIATHAIQGDPIDWVSTHRCHHRFVDSARDPH 126
           G+ ISVSYHRNLAH+SFKLPK +EY LAY A HA+QGDPIDWVSTHRCHH+FVD+ +DPH
Sbjct: 65  GLCISVSYHRNLAHKSFKLPKSMEYFLAYCAAHALQGDPIDWVSTHRCHHQFVDTEKDPH 124

Query: 127 SPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNIAMFYMKHGYPDNVTDLEKQRF 186
           SPI GFWFS +TW FDSYNLT+KVCPNYF ++QKV+R+I +++ KHG PDNV DLEKQ F
Sbjct: 125 SPIEGFWFSHITWLFDSYNLTKKVCPNYFTDFQKVDRSIFIWFSKHGRPDNVRDLEKQTF 184

Query: 187 YRFLHKTYWLHPLALSLLLYAIGGLPFIIWGMCVRFVYSLHVTSMVNSVCHIWGNQPWNT 246
           YRF+HKTY+LH + L++LLY +GGLPF+IWG CVR V  +HVT MVNSVCHIWG + WNT
Sbjct: 185 YRFIHKTYFLHHILLAILLYQVGGLPFLIWGTCVRIVVLMHVTFMVNSVCHIWGKRRWNT 244

Query: 247 GDLSKNNWMVALLTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYLIRFLQVIGLATNVKL 306
            DLS+NNW+V LL FGEGWHNNHHAFEYSAR G +WWQID+GWY IR LQ IG+A NVKL
Sbjct: 245 KDLSRNNWLVGLLAFGEGWHNNHHAFEYSARFGLQWWQIDFGWYTIRVLQAIGVAKNVKL 304

Query: 307 PS--EKQKL 314
           PS   KQ+L
Sbjct: 305 PSTIHKQRL 311

BLAST of CSPI07G15670.1 vs. TrEMBL
Match: A0A0A0LTL5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G227450 PE=3 SV=1)

HSP 1 Score: 419.5 bits (1077), Expect = 3.9e-114
Identity = 193/312 (61.86%), Postives = 240/312 (76.92%), Query Frame = 1

Query: 12  MVMKSPPRLFRRKKWTKFDRNVAFYFLSLHLLCILAPFYFSWTCLLLALGLSYLTG-MGI 71
           MV K+    F ++KWT  D+ +A +F+ LH +CILAPF+F+W    +A  L  +TG  GI
Sbjct: 4   MVKKADLITFGQRKWTNRDKYMAAFFIFLHFICILAPFHFNWNAFWVAFALYIITGCFGI 63

Query: 72  SVSYHRNLAHRSFKLPKWLEYSLAYIATHAIQGDPIDWVSTHRCHHRFVDSARDPHSPIY 131
           ++SYHRNL+HRSF+LPKWLEY  AY    A QGDPIDWVSTHRCHH+FVD+  DPHSPI 
Sbjct: 64  TISYHRNLSHRSFRLPKWLEYFFAYCGALAFQGDPIDWVSTHRCHHQFVDTENDPHSPIQ 123

Query: 132 GFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNIAMFYMKHGYPDNVTDLEKQRFYRFL 191
           GFWFS   W  DS  LTR+VCP YF +++  E+ I    +K+G P+NV DLEKQ FYRFL
Sbjct: 124 GFWFSYFIWILDSNALTRRVCPQYFIDHKDTEKTIFTVVLKYGRPNNVGDLEKQFFYRFL 183

Query: 192 HKTYWLHPLALSLLLYAIGGLPFIIWGMCVRFVYSLHVTSMVNSVCHIWGNQPWNTGDLS 251
            KTY+LH LAL++LLYA+GG PF+IWG  VR + ++HVT MVNS+CH +GNQPWNTGDLS
Sbjct: 184 RKTYFLHQLALAILLYAVGGTPFLIWGAFVRTIGTIHVTFMVNSICHTFGNQPWNTGDLS 243

Query: 252 KNNWMVALLTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYLIRFLQVIGLATNVKLPSE- 311
           +N W + L++FGEGWHNNHHAFEYSAR G EWWQID  WY+I FLQVIGLAT VK+PS+ 
Sbjct: 244 RNTWWMCLISFGEGWHNNHHAFEYSARQGLEWWQIDISWYIIWFLQVIGLATEVKVPSQS 303

Query: 312 -KQKLAALNKSK 321
            KQ+L AL+++K
Sbjct: 304 HKQRLQALDQTK 315

BLAST of CSPI07G15670.1 vs. TAIR10
Match: AT3G15850.1 (AT3G15850.1 fatty acid desaturase 5)

HSP 1 Score: 371.7 bits (953), Expect = 4.7e-103
Identity = 173/302 (57.28%), Postives = 214/302 (70.86%), Query Frame = 1

Query: 12  MVMKSPPRLFRRKKWTKFDRNVAFYFLSLHLLCILAPFYFSWTCLLLALGLSYLTGM-GI 71
           +V K    ++  ++W   D       LS+HLL +LAPF F+W  + +A GL  +TG+ GI
Sbjct: 84  LVKKKEKVVWWEREWKAMDFGAVAVVLSMHLLSLLAPFQFNWRAVSVAFGLYIVTGLLGI 143

Query: 72  SVSYHRNLAHRSFKLPKWLEYSLAYIATHAIQGDPIDWVSTHRCHHRFVDSARDPHSPIY 131
           ++S+HRNL+H++FKLPKWLEY  AY    A+QG+PIDWVSTHR HH+F DS RDPHSP+ 
Sbjct: 144 TLSFHRNLSHKAFKLPKWLEYLFAYCGAQALQGNPIDWVSTHRYHHQFCDSDRDPHSPLD 203

Query: 132 GFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNIAMFYMKHGYPDNVTDLEKQRFYRFL 191
           GFWFS M W FD+  +T++                       G P+NV DLEKQ FYRFL
Sbjct: 204 GFWFSHMNWMFDTNTITQRC----------------------GEPNNVGDLEKQPFYRFL 263

Query: 192 HKTYWLHPLALSLLLYAIGGLPFIIWGMCVRFVYSLHVTSMVNSVCHIWGNQPWNTGDLS 251
             TY LHPLAL++ LYA+GG PFI+WGM VR V+  H+T +VNS CH+WG Q WNTGDLS
Sbjct: 264 RTTYILHPLALAVALYAMGGFPFIVWGMGVRIVWVYHITWLVNSACHVWGKQAWNTGDLS 323

Query: 252 KNNWMVALLTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYLIRFLQVIGLATNVKLPSEK 311
           KNNW VA L FGEGWHNNHHAFE+SARHG EWWQ+D  WY+++FLQ IGLAT+VKLPSE 
Sbjct: 324 KNNWWVAALAFGEGWHNNHHAFEFSARHGLEWWQLDMTWYVVKFLQAIGLATDVKLPSEA 363

Query: 312 QK 313
           QK
Sbjct: 384 QK 363

BLAST of CSPI07G15670.1 vs. TAIR10
Match: AT3G15870.1 (AT3G15870.1 Fatty acid desaturase family protein)

HSP 1 Score: 333.6 bits (854), Expect = 1.4e-91
Identity = 157/301 (52.16%), Postives = 195/301 (64.78%), Query Frame = 1

Query: 11  EMVMKSPPRLFRRKKWTKFDRNVAFYFLSLHLLCILAPFYFSWTCLLLALGLSYLTGMGI 70
           E+V+    R F  + WT +D +    F+  HLL +LAPFYFSW    +   L ++ G+ I
Sbjct: 76  EVVVVRTKRSFWERSWTSWDVSKLVIFVGTHLLSLLAPFYFSWEAFWVFPWLVFINGICI 135

Query: 71  SVSYHRNLAHRSFKLPKWLEYSLAYIATHAIQGDPIDWVSTHRCHHRFVDSARDPHSPIY 130
           ++SYHRNL+HRSF LPKWLEY  AY    A QGDPI+WVS HR HH+  ++ RDPHSP  
Sbjct: 136 TLSYHRNLSHRSFDLPKWLEYLFAYGGVLAFQGDPIEWVSNHRYHHKHCETQRDPHSPTQ 195

Query: 131 GFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNIAMFYMKHGYPDNVTDLEKQRFYRFL 190
           GFWFS M W FD+ ++                          G  +NV DL +Q FYRFL
Sbjct: 196 GFWFSHMAWIFDTSSILENC----------------------GGEENVDDLVRQPFYRFL 255

Query: 191 HKTYWLHPLALSLLLYAIGGLPFIIWGMCVRFVYSLHVTSMVNSVCHIWGNQPWNTGDLS 250
            +T  LH +A S L Y  GG+P ++WG+ +     LH+T +VNSVCHIWG + WNT D S
Sbjct: 256 QRTVLLHMMAYSFLFYFCGGMPLLVWGIGITIAVRLHLTFLVNSVCHIWGTRAWNTSDFS 315

Query: 251 KNNWMVALLTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYLIRFLQVIGLATNVKLPSEK 310
           KNNW VA+LT GEGWHNNHHAFE+SARHG EWWQ+D  W LIRFL+ IGLATNVKLP+E 
Sbjct: 316 KNNWWVAILTLGEGWHNNHHAFEFSARHGLEWWQLDITWCLIRFLEAIGLATNVKLPTET 354

Query: 311 Q 312
           Q
Sbjct: 376 Q 354

BLAST of CSPI07G15670.1 vs. TAIR10
Match: AT1G06080.1 (AT1G06080.1 delta 9 desaturase 1)

HSP 1 Score: 325.5 bits (833), Expect = 3.8e-89
Identity = 154/294 (52.38%), Postives = 192/294 (65.31%), Query Frame = 1

Query: 19  RLFRRKKWTKFDRNVAFYFLSLHLLCILAPFYFSWTCLLLALGLSYLTGMGISVSYHRNL 78
           R    +KW + D   AF  L +H LC+LAPF F+W  L +AL +  + G+GI+VSYHRNL
Sbjct: 27  RAMWERKWKRLDIVKAFASLFVHFLCLLAPFNFTWPALRVALIVYTVGGLGITVSYHRNL 86

Query: 79  AHRSFKLPKWLEYSLAYIATHAIQGDPIDWVSTHRCHHRFVDSARDPHSPIYGFWFSQMT 138
           AHRSFK+PKWLEY  AY    AIQGDPIDWVSTHR HH+F DS RDPHSP  GFWFS + 
Sbjct: 87  AHRSFKVPKWLEYFFAYCGLLAIQGDPIDWVSTHRYHHQFTDSDRDPHSPNEGFWFSHLL 146

Query: 139 WFFDSYNLTRKVCPNYFNNYQKVERNIAMFYMKHGYPDNVTDLEKQRFYRFLHKTYWLHP 198
           W FD+  L  K                       G   NV DL++Q +Y+FL +T   H 
Sbjct: 147 WLFDTGYLVEKC----------------------GRRTNVEDLKRQWYYKFLQRTVLYHI 206

Query: 199 LALSLLLYAIGGLPFIIWGMCVRFVYSLHVTSMVNSVCHIWGNQPWNTGDLSKNNWMVAL 258
           L    LLY  GGL F+ WGM +      HVT ++NS+CH+WG++ W T D S+N W +++
Sbjct: 207 LTFGFLLYYFGGLSFLTWGMGIGVAMEHHVTCLINSLCHVWGSRTWKTNDTSRNVWWLSV 266

Query: 259 LTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYLIRFLQVIGLATNVKLPSEKQK 313
            +FGE WHNNHHAFE SAR G EWWQID  WY++RFL++IGLAT+VKLPSE Q+
Sbjct: 267 FSFGESWHNNHHAFESSARQGLEWWQIDISWYIVRFLEIIGLATDVKLPSESQR 298

BLAST of CSPI07G15670.1 vs. TAIR10
Match: AT2G31360.1 (AT2G31360.1 16:0delta9 desaturase 2)

HSP 1 Score: 319.7 bits (818), Expect = 2.1e-87
Identity = 150/293 (51.19%), Postives = 191/293 (65.19%), Query Frame = 1

Query: 20  LFRRKKWTKFDRNVAFYFLSLHLLCILAPFYFSWTCLLLALGLSYLTGMGISVSYHRNLA 79
           +F  ++W + D        ++H L +LAPFYF+W+ L +      + G+GI+VSYHRNLA
Sbjct: 30  VFWDRRWRRLDYVKFSASFTVHSLALLAPFYFTWSALWVTFLFYTIGGLGITVSYHRNLA 89

Query: 80  HRSFKLPKWLEYSLAYIATHAIQGDPIDWVSTHRCHHRFVDSARDPHSPIYGFWFSQMTW 139
           HRSFK+PKWLEY LAY A  AIQGDPIDWVSTHR HH+F DS RDPHSP  GFWFS + W
Sbjct: 90  HRSFKVPKWLEYLLAYCALLAIQGDPIDWVSTHRYHHQFTDSERDPHSPKEGFWFSHLLW 149

Query: 140 FFDSYNLTRKVCPNYFNNYQKVERNIAMFYMKHGYPDNVTDLEKQRFYRFLHKTYWLHPL 199
            +DS  L  K                       G   NV DL++Q FYRFL KT   H L
Sbjct: 150 IYDSAYLVSKC----------------------GRRANVEDLKRQWFYRFLQKTVLFHIL 209

Query: 200 ALSLLLYAIGGLPFIIWGMCVRFVYSLHVTSMVNSVCHIWGNQPWNTGDLSKNNWMVALL 259
            L   L+ +GG+ F+ WGM V     +HVT ++NS+CHIWG + W T D S+N W +++ 
Sbjct: 210 GLGFFLFYLGGMSFVTWGMGVGAALEVHVTCLINSLCHIWGTRTWKTNDTSRNVWWLSVF 269

Query: 260 TFGEGWHNNHHAFEYSARHGHEWWQIDYGWYLIRFLQVIGLATNVKLPSEKQK 313
           +FGE WHNNHHAFE SAR G EWWQID  WY++RF ++IGLAT+VK+P+E Q+
Sbjct: 270 SFGESWHNNHHAFESSARQGLEWWQIDISWYIVRFFEIIGLATDVKVPTEAQR 300

BLAST of CSPI07G15670.1 vs. TAIR10
Match: AT1G06100.1 (AT1G06100.1 Fatty acid desaturase family protein)

HSP 1 Score: 311.2 bits (796), Expect = 7.5e-85
Identity = 159/314 (50.64%), Postives = 199/314 (63.38%), Query Frame = 1

Query: 1   MDEVSKE--KPKEMVMKSPPRLFRRKKWTKFDRNVAFYFLSLHLLCILAPFYFSWTCLLL 60
           M E +K+    ++  ++   R +  +KWT+FD   A    ++HLLC+LAPF + W     
Sbjct: 1   MSETTKDDGSSQKKSVRKEKRAYVLRKWTQFDVGRASTVGTVHLLCLLAPFNYKWEAFRF 60

Query: 61  ALGLSYLTGMGISVSYHRNLAHRSFKLPKWLEYSLAYIATHAIQGDPIDWVSTHRCHHRF 120
            + L+ LT + I+ SYHRNL HRSFKLPKWLEY  AY A  A+QGDP+DWVS HR HH+F
Sbjct: 61  GIILAILTNLCITFSYHRNLTHRSFKLPKWLEYPFAYSALLALQGDPLDWVSIHRFHHQF 120

Query: 121 VDSARDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNIAMFYMKHGYPDNV 180
            DS RDPHSPI GFWFS + W FD+ +  R+ C                     G  +NV
Sbjct: 121 TDSDRDPHSPIEGFWFSHVLWIFDT-DYIREKC---------------------GRRNNV 180

Query: 181 TDLEKQRFYRFLHKTYWLHPLALSLLLYAIGGLPFIIWGMCVRFVYSLHVTSMVNSVCHI 240
            DL++Q FYRFL KT  LH LA   L+Y  GGLP++ W +    V   H T +VNS CHI
Sbjct: 181 MDLKQQWFYRFLKKTLVLHILAFWTLIYLWGGLPYLTWTVGFGGVIGYHGTWLVNSACHI 240

Query: 241 WGNQPWNTGDLSKNNWMVALLTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYLIRFLQVI 300
            G+Q W T D S+N W +ALLT GE WHNNHHAFE SARHG EW+Q+D  WYLI F Q +
Sbjct: 241 CGSQAWQTNDTSRNVWWLALLTMGESWHNNHHAFETSARHGLEWYQLDITWYLIWFFQAL 292

Query: 301 GLATNVKLPSEKQK 313
           GLATNVKLP++ QK
Sbjct: 301 GLATNVKLPTDAQK 292

BLAST of CSPI07G15670.1 vs. NCBI nr
Match: gi|778727442|ref|XP_011659261.1| (PREDICTED: palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Cucumis sativus])

HSP 1 Score: 698.7 bits (1802), Expect = 4.7e-198
Identity = 326/329 (99.09%), Postives = 328/329 (99.70%), Query Frame = 1

Query: 1   MDEVSKEKPKEMVMKSPPRLFRRKKWTKFDRNVAFYFLSLHLLCILAPFYFSWTCLLLAL 60
           MDEVSKEKPKEMVMKSPPRLFRRKKWTKFDRNVAFYFLSLHLLCILAPFYFSWTCLLLAL
Sbjct: 1   MDEVSKEKPKEMVMKSPPRLFRRKKWTKFDRNVAFYFLSLHLLCILAPFYFSWTCLLLAL 60

Query: 61  GLSYLTGMGISVSYHRNLAHRSFKLPKWLEYSLAYIATHAIQGDPIDWVSTHRCHHRFVD 120
           GLSYLTGMGISVSYHRNLAHRSFKLPKWLEYSLAYIATHAIQGDPIDWVSTHRCHHRFVD
Sbjct: 61  GLSYLTGMGISVSYHRNLAHRSFKLPKWLEYSLAYIATHAIQGDPIDWVSTHRCHHRFVD 120

Query: 121 SARDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNIAMFYMKHGYPDNVTD 180
           SARDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNI MFYMKHGYPDNVTD
Sbjct: 121 SARDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNIFMFYMKHGYPDNVTD 180

Query: 181 LEKQRFYRFLHKTYWLHPLALSLLLYAIGGLPFIIWGMCVRFVYSLHVTSMVNSVCHIWG 240
           LEKQ+FYRF+HKTYWLHPLALSLLLYAIGGLPFIIWGMCVRFVYSLHVTSMVNSVCHIWG
Sbjct: 181 LEKQKFYRFIHKTYWLHPLALSLLLYAIGGLPFIIWGMCVRFVYSLHVTSMVNSVCHIWG 240

Query: 241 NQPWNTGDLSKNNWMVALLTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYLIRFLQVIGL 300
           NQPWNTGDLSKNNWMVALLTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYLIRFLQVIGL
Sbjct: 241 NQPWNTGDLSKNNWMVALLTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYLIRFLQVIGL 300

Query: 301 ATNVKLPSEKQKLAALNKSKPIFLKLMID 330
           ATNVKLPSEKQKLAALNKSKPIFLKLMID
Sbjct: 301 ATNVKLPSEKQKLAALNKSKPIFLKLMID 329

BLAST of CSPI07G15670.1 vs. NCBI nr
Match: gi|659100771|ref|XP_008451258.1| (PREDICTED: palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Cucumis melo])

HSP 1 Score: 645.2 bits (1663), Expect = 6.2e-182
Identity = 297/329 (90.27%), Postives = 310/329 (94.22%), Query Frame = 1

Query: 1   MDEVSKEKPKEMVMKSPPRLFRRKKWTKFDRNVAFYFLSLHLLCILAPFYFSWTCLLLAL 60
           M+E SKE+ KEMVM SP RLF+RKKWTKFD+NVAFYFL LHLLCILAPFYFSWTCL L+L
Sbjct: 1   MEEASKEESKEMVMNSPSRLFKRKKWTKFDKNVAFYFLLLHLLCILAPFYFSWTCLFLSL 60

Query: 61  GLSYLTGMGISVSYHRNLAHRSFKLPKWLEYSLAYIATHAIQGDPIDWVSTHRCHHRFVD 120
           GL+ LTGMGISVSYHRNLAHRSFKLPKWLEYSLAYIA HAIQGDPIDWVSTHRCHHRFVD
Sbjct: 61  GLANLTGMGISVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVD 120

Query: 121 SARDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNIAMFYMKHGYPDNVTD 180
           S RDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNN+QKVERN  MFYMKHGY DNVTD
Sbjct: 121 SERDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNFQKVERNAFMFYMKHGYADNVTD 180

Query: 181 LEKQRFYRFLHKTYWLHPLALSLLLYAIGGLPFIIWGMCVRFVYSLHVTSMVNSVCHIWG 240
           LEKQRFYRF+HKTYWLHPLALSLLLYAIGGLPFIIWGMCVRFV SLH TSM+NSVCHIWG
Sbjct: 181 LEKQRFYRFIHKTYWLHPLALSLLLYAIGGLPFIIWGMCVRFVISLHFTSMINSVCHIWG 240

Query: 241 NQPWNTGDLSKNNWMVALLTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYLIRFLQVIGL 300
           NQPWNTGDLSKNNW+VA+ TFGEGWHNNHHAFEYSARHGHEWWQIDYGWY+I+FLQVIG 
Sbjct: 241 NQPWNTGDLSKNNWIVAIFTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYIIKFLQVIGF 300

Query: 301 ATNVKLPSEKQKLAALNKSKPIFLKLMID 330
           ATNVKLPSEKQK AALNKSKP FLK MID
Sbjct: 301 ATNVKLPSEKQKWAALNKSKPTFLKPMID 329

BLAST of CSPI07G15670.1 vs. NCBI nr
Match: gi|778730379|ref|XP_011659770.1| (PREDICTED: palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Cucumis sativus])

HSP 1 Score: 634.4 bits (1635), Expect = 1.1e-178
Identity = 294/330 (89.09%), Postives = 310/330 (93.94%), Query Frame = 1

Query: 1   MDEVSKEKPKEMVMKSPPRLFRRKKWTKFDRNVAFYFLSLHLLCILAP-FYFSWTCLLLA 60
           M+  SKE PKEMVM SP RLF+RKKWTKFD+NVAFYFL LHLL ILAP FYFSW CLLL+
Sbjct: 1   MEGASKEDPKEMVMSSPSRLFKRKKWTKFDKNVAFYFLLLHLLSILAPLFYFSWGCLLLS 60

Query: 61  LGLSYLTGMGISVSYHRNLAHRSFKLPKWLEYSLAYIATHAIQGDPIDWVSTHRCHHRFV 120
           LGL+ LTGM ISVSYHRNLAHRSFKLPKWLEYSLAYIA HAIQGDPIDWVSTHRCHHRFV
Sbjct: 61  LGLANLTGMAISVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFV 120

Query: 121 DSARDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNIAMFYMKHGYPDNVT 180
           DS RDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNI MFYMKHGYPDNVT
Sbjct: 121 DSERDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNIFMFYMKHGYPDNVT 180

Query: 181 DLEKQRFYRFLHKTYWLHPLALSLLLYAIGGLPFIIWGMCVRFVYSLHVTSMVNSVCHIW 240
           DLEKQ+FYRF+HKTYWLHPLALSLLLYA+GGLPFIIWG+CVRFV SLH TSM+NSVCHIW
Sbjct: 181 DLEKQKFYRFIHKTYWLHPLALSLLLYALGGLPFIIWGVCVRFVLSLHFTSMINSVCHIW 240

Query: 241 GNQPWNTGDLSKNNWMVALLTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYLIRFLQVIG 300
           GNQPWNTGDLSKNNW+VA+ TFGEGWHNNHHAFEYSARHGHEWWQID GWY+I+FLQVIG
Sbjct: 241 GNQPWNTGDLSKNNWIVAIFTFGEGWHNNHHAFEYSARHGHEWWQIDNGWYIIKFLQVIG 300

Query: 301 LATNVKLPSEKQKLAALNKSKPIFLKLMID 330
           LATNVKLPSEKQK+AALNKSKP FL+ MID
Sbjct: 301 LATNVKLPSEKQKMAALNKSKPTFLRPMID 330

BLAST of CSPI07G15670.1 vs. NCBI nr
Match: gi|659100773|ref|XP_008451259.1| (PREDICTED: palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Cucumis melo])

HSP 1 Score: 634.0 bits (1634), Expect = 1.4e-178
Identity = 297/329 (90.27%), Postives = 308/329 (93.62%), Query Frame = 1

Query: 2   DEVSKEKPKEMV-MKSPPRLFRRKKWTKFDRNVAFYFLSLHLLCILAPFYFSWTCLLLAL 61
           +EVSKEKPKE++ MKSPP+LFRRKKWTKFDRN AFYFL+ HLLCILAPF FSWT LLLAL
Sbjct: 3   NEVSKEKPKEIILMKSPPKLFRRKKWTKFDRNFAFYFLTFHLLCILAPFCFSWTRLLLAL 62

Query: 62  GLSYLTGMGISVSYHRNLAHRSFKLPKWLEYSLAYIATHAIQGDPIDWVSTHRCHHRFVD 121
           GLS L+GMGISVSYHRNLAHRSFKLPKW EYSLAYIA HAIQGDPIDWVSTHRCHHRFVD
Sbjct: 63  GLSCLSGMGISVSYHRNLAHRSFKLPKWFEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVD 122

Query: 122 SARDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNIAMFYMKHGYPDNVTD 181
           S RDPHSPIYGFWFSQMTW FDSYNLTRKVCPNYFNNYQK ERNI M YMKHGYPDNV D
Sbjct: 123 SERDPHSPIYGFWFSQMTWLFDSYNLTRKVCPNYFNNYQKAERNIFMLYMKHGYPDNVPD 182

Query: 182 LEKQRFYRFLHKTYWLHPLALSLLLYAIGGLPFIIWGMCVRFVYSLHVTSMVNSVCHIWG 241
           LEKQ FYRF+HKTYWLHPLALSLLLYAIGGLPFIIWGMCVRFVYS+HVTSMVNSVCH+WG
Sbjct: 183 LEKQWFYRFIHKTYWLHPLALSLLLYAIGGLPFIIWGMCVRFVYSVHVTSMVNSVCHMWG 242

Query: 242 NQPWNTGDLSKNNWMVALLTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYLIRFLQVIGL 301
           NQPWNTGDLSKNNWMVALLTFGEGWHNNHHAFEYSARHG EWWQIDYGWY IRFL+VIGL
Sbjct: 243 NQPWNTGDLSKNNWMVALLTFGEGWHNNHHAFEYSARHGLEWWQIDYGWYFIRFLEVIGL 302

Query: 302 ATNVKLPSEKQKLAALNKSKPIFLKLMID 330
           ATNVK PSEKQKLAALNKSKP FLK  I+
Sbjct: 303 ATNVKFPSEKQKLAALNKSKPTFLKPTIN 331

BLAST of CSPI07G15670.1 vs. NCBI nr
Match: gi|700189514|gb|KGN44747.1| (hypothetical protein Csa_7G377870 [Cucumis sativus])

HSP 1 Score: 538.5 bits (1386), Expect = 8.2e-150
Identity = 241/262 (91.98%), Postives = 252/262 (96.18%), Query Frame = 1

Query: 68  MGISVSYHRNLAHRSFKLPKWLEYSLAYIATHAIQGDPIDWVSTHRCHHRFVDSARDPHS 127
           M ISVSYHRNLAHRSFKLPKWLEYSLAYIA HAIQGDPIDWVSTHRCHHRFVDS RDPHS
Sbjct: 1   MAISVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVDSERDPHS 60

Query: 128 PIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNIAMFYMKHGYPDNVTDLEKQRFY 187
           PIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNI MFYMKHGYPDNVTDLEKQ+FY
Sbjct: 61  PIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNIFMFYMKHGYPDNVTDLEKQKFY 120

Query: 188 RFLHKTYWLHPLALSLLLYAIGGLPFIIWGMCVRFVYSLHVTSMVNSVCHIWGNQPWNTG 247
           RF+HKTYWLHPLALSLLLYA+GGLPFIIWG+CVRFV SLH TSM+NSVCHIWGNQPWNTG
Sbjct: 121 RFIHKTYWLHPLALSLLLYALGGLPFIIWGVCVRFVLSLHFTSMINSVCHIWGNQPWNTG 180

Query: 248 DLSKNNWMVALLTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYLIRFLQVIGLATNVKLP 307
           DLSKNNW+VA+ TFGEGWHNNHHAFEYSARHGHEWWQID GWY+I+FLQVIGLATNVKLP
Sbjct: 181 DLSKNNWIVAIFTFGEGWHNNHHAFEYSARHGHEWWQIDNGWYIIKFLQVIGLATNVKLP 240

Query: 308 SEKQKLAALNKSKPIFLKLMID 330
           SEKQK+AALNKSKP FL+ MID
Sbjct: 241 SEKQKMAALNKSKPTFLRPMID 262

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
ADS3_ARATH8.3e-10257.28Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic OS=Arab... [more]
ADS32_ARATH2.5e-9052.16Probable lipid desaturase ADS3.2, chloroplastic OS=Arabidopsis thaliana GN=ADS3.... [more]
ADS1_ARATH6.8e-8852.38Delta-9 acyl-lipid desaturase 1 OS=Arabidopsis thaliana GN=ADS1 PE=2 SV=1[more]
ADS2_ARATH3.7e-8651.19Delta-9 acyl-lipid desaturase 2 OS=Arabidopsis thaliana GN=ADS2 PE=2 SV=2[more]
ICO5D_LIMDO6.4e-8650.31Icosanoyl-CoA 5-desaturase (Fragment) OS=Limnanthes douglasii PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KAB1_CUCSA3.3e-19899.09Uncharacterized protein OS=Cucumis sativus GN=Csa_7G376370 PE=3 SV=1[more]
A0A0A0K8P8_CUCSA5.7e-15091.98Uncharacterized protein OS=Cucumis sativus GN=Csa_7G377870 PE=3 SV=1[more]
A0A0A0KVH2_CUCSA1.2e-12665.33Uncharacterized protein OS=Cucumis sativus GN=Csa_4G006060 PE=3 SV=1[more]
A0A0A0KYH4_CUCSA1.4e-12466.34Uncharacterized protein OS=Cucumis sativus GN=Csa_4G006130 PE=3 SV=1[more]
A0A0A0LTL5_CUCSA3.9e-11461.86Uncharacterized protein OS=Cucumis sativus GN=Csa_1G227450 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G15850.14.7e-10357.28 fatty acid desaturase 5[more]
AT3G15870.11.4e-9152.16 Fatty acid desaturase family protein[more]
AT1G06080.13.8e-8952.38 delta 9 desaturase 1[more]
AT2G31360.12.1e-8751.19 16:0delta9 desaturase 2[more]
AT1G06100.17.5e-8550.64 Fatty acid desaturase family protein[more]
Match NameE-valueIdentityDescription
gi|778727442|ref|XP_011659261.1|4.7e-19899.09PREDICTED: palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplas... [more]
gi|659100771|ref|XP_008451258.1|6.2e-18290.27PREDICTED: palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplas... [more]
gi|778730379|ref|XP_011659770.1|1.1e-17889.09PREDICTED: palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplas... [more]
gi|659100773|ref|XP_008451259.1|1.4e-17890.27PREDICTED: palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplas... [more]
gi|700189514|gb|KGN44747.1|8.2e-15091.98hypothetical protein Csa_7G377870 [Cucumis sativus][more]
The following terms have been associated with this mRNA:
Vocabulary: INTERPRO
TermDefinition
IPR005804FA_desaturase_dom
IPR015876Acyl-CoA_DS
Vocabulary: Biological Process
TermDefinition
GO:0006629lipid metabolic process
GO:0055114oxidation-reduction process
Vocabulary: Molecular Function
TermDefinition
GO:0016717oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006629 lipid metabolic process
biological_process GO:0055114 oxidation-reduction process
biological_process GO:0006633 fatty acid biosynthetic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0016717 oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
CSPI07G15670CSPI07G15670gene


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
CSPI07G15670.1CSPI07G15670.1-proteinpolypeptide


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
CSPI07G15670.1.utr3p1CSPI07G15670.1.utr3p1three_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
CSPI07G15670.1.cds4CSPI07G15670.1.cds4CDS
CSPI07G15670.1.cds3CSPI07G15670.1.cds3CDS
CSPI07G15670.1.cds2CSPI07G15670.1.cds2CDS
CSPI07G15670.1.cds1CSPI07G15670.1.cds1CDS


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
CSPI07G15670.1.utr5p1CSPI07G15670.1.utr5p1five_prime_UTR


Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005804Fatty acid desaturase domainPFAMPF00487FA_desaturasecoord: 52..290
score: 9.0
IPR015876Fatty acid desaturase, type 1, corePRINTSPR00075FACDDSATRASEcoord: 112..141
score: 3.2E-11coord: 75..95
score: 3.2E-11coord: 52..74
score: 3.2
NoneNo IPR availablePANTHERPTHR11351ACYL-COA DESATURASEcoord: 11..329
score: 1.9E
NoneNo IPR availablePANTHERPTHR11351:SF2DELTA-9 ACYL-LIPID DESATURASE 1-RELATEDcoord: 11..329
score: 1.9E