CSPI07G12660.1 (mRNA) Wild cucumber (PI 183967) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CSPI07G12660.1
Type	mRNA
Organism	Cucumis sativus (Wild cucumber (PI 183967))
Description	Restriction endonuclease, type II-like protein
Location	Chr7 : 10952373 .. 10953381 (-)
Sequence length	909

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTGAAGCCGAGGTTCTTCTCTATTTACTGGGACTTTCTTCTCTGAGCTGATTCTGTCCACTAACTCTGGATGGCTCTTGATCTTTGTGTCTTTCGGTTCCTTTAATTTCAGAAACAATCGATGTTCAATTGCAAAAAGATATTATTTGCCTGTAGTCAAGCTATTGGGAACTGTTCTATACGGAATTTCAATTCCGTTTCTTCTTCTTCTTTACAATTTGAAACGGTTAATCACTATTCTGTTCTTCAGTCCACTAGTTTTCAGCATTGGTTCAAAAATTGGCAAGAGCTTCGAAAGCATAAGTTAACAGCAAGTACTTTTGCTGGGGCAATTGGGTTTTGGCCTCGTCGAAGGACTCAACTATGGTTGGAGAAACTTGGGGCAATTGATCAATTTTGTGGTAATCTTGCTACTTGTTGGAGTAATATGAAAGAAGAAGAAGCACTCGAAAGATACAAGCTTATTACTGGAAACTCTGTTTTGTTTCCTGAATTTCAAGTCTATGGTAAAGCAAACTCGGAAGATGATTGGTTGGCTGCTTCACCTGATGGTGCAATTGATAAAATGATTTATGGATTGCCTTCACAAGGTGTTTTGGAGATTAAGTGCCCATTTTTTAATGGTGATATGAGAAATGCTTCACCATGGTCACGAGTTCCTCTTTACTGTATTCCTCAGGCTCAAGGTTTGATGGAAATAATGGATAGAGATTGGATGGACTTTTATGTTTGGACTCCTAATGGTAGTAGTTTGTTTAGATTGTATCGAGATGTCGAATATTGGGACGTTTTGAAGATTGCTTTGTCGGATTTTTGGTGGAAGCATGTCCAACCTGCAAGGGAGATGTGTAGTAAATATGTCGTTACAAATCCCCTCGTTGAGCTGAAGTCTCTTAGGCCATCACCCAGGCATGAACTGTGTAGTTATATAGTTTGTGAGAGCAGACGGGTTGTCAATAATTCTAAGTTGCTCTTGCGTGAATTTGATGGGAGACTTCAAACCTAA

mRNA sequence

ATGGGTGAAGCCGAGAAACAATCGATGTTCAATTGCAAAAAGATATTATTTGCCTGTAGTCAAGCTATTGGGAACTGTTCTATACGGAATTTCAATTCCGTTTCTTCTTCTTCTTTACAATTTGAAACGGTTAATCACTATTCTGTTCTTCAGTCCACTAGTTTTCAGCATTGGTTCAAAAATTGGCAAGAGCTTCGAAAGCATAAGTTAACAGCAAGTACTTTTGCTGGGGCAATTGGGTTTTGGCCTCGTCGAAGGACTCAACTATGGTTGGAGAAACTTGGGGCAATTGATCAATTTTGTGGTAATCTTGCTACTTGTTGGAGTAATATGAAAGAAGAAGAAGCACTCGAAAGATACAAGCTTATTACTGGAAACTCTGTTTTGTTTCCTGAATTTCAAGTCTATGGTAAAGCAAACTCGGAAGATGATTGGTTGGCTGCTTCACCTGATGGTGCAATTGATAAAATGATTTATGGATTGCCTTCACAAGGTGTTTTGGAGATTAAGTGCCCATTTTTTAATGGTGATATGAGAAATGCTTCACCATGGTCACGAGTTCCTCTTTACTGTATTCCTCAGGCTCAAGGTTTGATGGAAATAATGGATAGAGATTGGATGGACTTTTATGTTTGGACTCCTAATGGTAGTAGTTTGTTTAGATTGTATCGAGATGTCGAATATTGGGACGTTTTGAAGATTGCTTTGTCGGATTTTTGGTGGAAGCATGTCCAACCTGCAAGGGAGATGTGTAGTAAATATGTCGTTACAAATCCCCTCGTTGAGCTGAAGTCTCTTAGGCCATCACCCAGGCATGAACTGTGTAGTTATATAGTTTGTGAGAGCAGACGGGTTGTCAATAATTCTAAGTTGCTCTTGCGTGAATTTGATGGGAGACTTCAAACCTAA

Coding sequence (CDS)

ATGGGTGAAGCCGAGAAACAATCGATGTTCAATTGCAAAAAGATATTATTTGCCTGTAGTCAAGCTATTGGGAACTGTTCTATACGGAATTTCAATTCCGTTTCTTCTTCTTCTTTACAATTTGAAACGGTTAATCACTATTCTGTTCTTCAGTCCACTAGTTTTCAGCATTGGTTCAAAAATTGGCAAGAGCTTCGAAAGCATAAGTTAACAGCAAGTACTTTTGCTGGGGCAATTGGGTTTTGGCCTCGTCGAAGGACTCAACTATGGTTGGAGAAACTTGGGGCAATTGATCAATTTTGTGGTAATCTTGCTACTTGTTGGAGTAATATGAAAGAAGAAGAAGCACTCGAAAGATACAAGCTTATTACTGGAAACTCTGTTTTGTTTCCTGAATTTCAAGTCTATGGTAAAGCAAACTCGGAAGATGATTGGTTGGCTGCTTCACCTGATGGTGCAATTGATAAAATGATTTATGGATTGCCTTCACAAGGTGTTTTGGAGATTAAGTGCCCATTTTTTAATGGTGATATGAGAAATGCTTCACCATGGTCACGAGTTCCTCTTTACTGTATTCCTCAGGCTCAAGGTTTGATGGAAATAATGGATAGAGATTGGATGGACTTTTATGTTTGGACTCCTAATGGTAGTAGTTTGTTTAGATTGTATCGAGATGTCGAATATTGGGACGTTTTGAAGATTGCTTTGTCGGATTTTTGGTGGAAGCATGTCCAACCTGCAAGGGAGATGTGTAGTAAATATGTCGTTACAAATCCCCTCGTTGAGCTGAAGTCTCTTAGGCCATCACCCAGGCATGAACTGTGTAGTTATATAGTTTGTGAGAGCAGACGGGTTGTCAATAATTCTAAGTTGCTCTTGCGTGAATTTGATGGGAGACTTCAAACCTAA

Homology

[Close]

BLAST of CSPI07G12660.1 vs. TrEMBL
Match: A0A0A0K4T5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G302370 PE=4 SV=1)

HSP 1 Score: 641.7 bits (1654), Expect = 4.4e-181
Identity = 298/302 (98.68%), Postives = 302/302 (100.00%), Query Frame = 1


Query: 1   MGEAEKQSMFNCKKILFACSQAIGNCSIRNFNSVSSSSLQFETVNHYSVLQSTSFQHWFK 60
           MGEAEKQSMFNCKKILFACSQAIGNCSIRNFNSVSSSSLQFETVNHYSVLQSTSFQHWFK
Sbjct: 1   MGEAEKQSMFNCKKILFACSQAIGNCSIRNFNSVSSSSLQFETVNHYSVLQSTSFQHWFK 60

Query: 61  NWQELRKHKLTASTFAGAIGFWPRRRTQLWLEKLGAIDQFCGNLATCWSNMKEEEALERY 120
           NWQELRKHKLTASTFAGAIGFWPRRRTQLWLEKLGAIDQFCGNLATCWSNMKEEEALERY
Sbjct: 61  NWQELRKHKLTASTFAGAIGFWPRRRTQLWLEKLGAIDQFCGNLATCWSNMKEEEALERY 120

Query: 121 KLITGNSVLFPEFQVYGKANSEDDWLAASPDGAIDKMIYGLPSQGVLEIKCPFFNGDMRN 180
           KLITGNSVLFPEFQVYGKANSEDDWLAASPDGAIDKM+YGLPS+GVLEIKCPFFNGDMRN
Sbjct: 121 KLITGNSVLFPEFQVYGKANSEDDWLAASPDGAIDKMVYGLPSRGVLEIKCPFFNGDMRN 180

Query: 181 ASPWSRVPLYCIPQAQGLMEIMDRDWMDFYVWTPNGSSLFRLYRDVEYWDVLKIALSDFW 240
           ASPWSRVPLYCIPQAQGLMEIMDRDWMDFYVWTPNGSSLFRLYRDVEYWDVLKIALSDFW
Sbjct: 181 ASPWSRVPLYCIPQAQGLMEIMDRDWMDFYVWTPNGSSLFRLYRDVEYWDVLKIALSDFW 240

Query: 241 WKHVQPAREMCSKYVVTNPLVELKSLRPSPRHELCSYIVCESRRVVNNSKLLLREFDGRL 300
           WKHVQPAREMCSKYVVTNPL+ELKSLRPSPRHELCSYIVCES+RVVNNSKLLLREFDGRL
Sbjct: 241 WKHVQPAREMCSKYVVTNPLIELKSLRPSPRHELCSYIVCESKRVVNNSKLLLREFDGRL 300

Query: 301 QT 303
           QT
Sbjct: 301 QT 302

[Close]

BLAST of CSPI07G12660.1 vs. TrEMBL
Match: A0A0A0KG47_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G405910 PE=4 SV=1)

HSP 1 Score: 607.1 bits (1564), Expect = 1.2e-170
Identity = 281/294 (95.58%), Postives = 288/294 (97.96%), Query Frame = 1


Query: 9   MFNCKKILFACSQAIGNCSIRNFNSVSSSSLQFETVNHYSVLQSTSFQHWFKNWQELRKH 68
           MFN KKILFACSQAIGNCS+RNFNSVS SSLQFET NHYSVLQS+SFQHWFKNWQELRKH
Sbjct: 1   MFNSKKILFACSQAIGNCSLRNFNSVSFSSLQFETGNHYSVLQSSSFQHWFKNWQELRKH 60

Query: 69  KLTASTFAGAIGFWPRRRTQLWLEKLGAIDQFCGNLATCWSNMKEEEALERYKLITGNSV 128
           KLTASTFAGAIGFWPRRRTQLWLEKLGAIDQFCGNLATCWSNMKEEEALERYKLITGNSV
Sbjct: 61  KLTASTFAGAIGFWPRRRTQLWLEKLGAIDQFCGNLATCWSNMKEEEALERYKLITGNSV 120

Query: 129 LFPEFQVYGKANSEDDWLAASPDGAIDKMIYGLPSQGVLEIKCPFFNGDMRNASPWSRVP 188
           LFPEFQVYGKANSEDDWLAASPDGAIDKM+YGLPS+GVLEIKCPFFNGD+RNA PWSRVP
Sbjct: 121 LFPEFQVYGKANSEDDWLAASPDGAIDKMVYGLPSRGVLEIKCPFFNGDLRNALPWSRVP 180

Query: 189 LYCIPQAQGLMEIMDRDWMDFYVWTPNGSSLFRLYRDVEYWDVLKIALSDFWWKHVQPAR 248
            YCIPQAQGLMEIMDRDWMDFYVWTPNGSSLFRLYRD EYWDVLKIALSDFWWKHVQPAR
Sbjct: 181 RYCIPQAQGLMEIMDRDWMDFYVWTPNGSSLFRLYRDPEYWDVLKIALSDFWWKHVQPAR 240

Query: 249 EMCSKYVVTNPLVELKSLRPSPRHELCSYIVCESRRVVNNSKLLLREFDGRLQT 303
           EMCSKYV+TNPLVELKSLRPSPRHELCSYIVCES+RVVNNSKLLLREFDGRLQT
Sbjct: 241 EMCSKYVITNPLVELKSLRPSPRHELCSYIVCESKRVVNNSKLLLREFDGRLQT 294

[Close]

BLAST of CSPI07G12660.1 vs. TrEMBL
Match: A0A061F0P2_THECC (DNA-directed RNA polymerase subunit beta OS=Theobroma cacao GN=TCM_025578 PE=4 SV=1)

HSP 1 Score: 460.3 bits (1183), Expect = 1.8e-126
Identity = 203/255 (79.61%), Postives = 232/255 (90.98%), Query Frame = 1


Query: 47  YSVLQSTSFQHWFKNWQELRKHKLTASTFAGAIGFWPRRRTQLWLEKLGAIDQFCGNLAT 106
           +S+LQS+S QHWFKNWQE RK KLTASTF+GAIGFWP RR QLWLEK+GAI+ F GNLAT
Sbjct: 89  HSILQSSSLQHWFKNWQEQRKQKLTASTFSGAIGFWPCRRAQLWLEKIGAIEPFSGNLAT 148

Query: 107 CWSNMKEEEALERYKLITGNSVLFPEFQVYGKANSEDDWLAASPDGAIDKMIYGLPSQGV 166
           CWSN+KEEEALERYKLITGN+V FPEFQVYGK ++E+ WLAASPDG +D+ +YGLP +GV
Sbjct: 149 CWSNIKEEEALERYKLITGNTVSFPEFQVYGKMDAEEGWLAASPDGLVDRFVYGLPLRGV 208

Query: 167 LEIKCPFFNGDMRNASPWSRVPLYCIPQAQGLMEIMDRDWMDFYVWTPNGSSLFRLYRDV 226
           LEIKCPFF GDM  ASPW R+PLYCIPQAQGLMEIMDRDWMDFYVWTP GSSLFR+YRDV
Sbjct: 209 LEIKCPFFGGDMSKASPWRRIPLYCIPQAQGLMEIMDRDWMDFYVWTPKGSSLFRIYRDV 268

Query: 227 EYWDVLKIALSDFWWKHVQPAREMCSKYVVTNPLVELKSLRPSPRHELCSYIVCESRRVV 286
           EYWDVLK+ALSDFWWKHVQPA+E+CSKYV+T+PL ELKSLRP+PRHEL SYIV ES+RVV
Sbjct: 269 EYWDVLKVALSDFWWKHVQPAKEICSKYVITDPLRELKSLRPAPRHELLSYIVYESKRVV 328

Query: 287 NNSKLLLREFDGRLQ 302
           +NS LL+RE +G+L+
Sbjct: 329 DNSNLLIREINGQLK 343

[Close]

BLAST of CSPI07G12660.1 vs. TrEMBL
Match: F6I2K8_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_16s0013g00380 PE=4 SV=1)

HSP 1 Score: 453.4 bits (1165), Expect = 2.2e-124
Identity = 205/260 (78.85%), Postives = 228/260 (87.69%), Query Frame = 1


Query: 43  TVNH-YSVLQSTSFQHWFKNWQELRKHKLTASTFAGAIGFWPRRRTQLWLEKLGAIDQFC 102
           T  H YSVLQS+ FQHWFKNWQE RKHKLTASTF GA+GFWPRRR QLWLEKLGA   F 
Sbjct: 5   TAGHTYSVLQSSGFQHWFKNWQEQRKHKLTASTFGGAVGFWPRRRVQLWLEKLGATKPFS 64

Query: 103 GNLATCWSNMKEEEALERYKLITGNSVLFPEFQVYGKANSEDDWLAASPDGAIDKMIYGL 162
           GNLATCWSN+KEEEALERYKLITGN+VLFPEFQVYGK + ED+WLAASPDG +D ++YGL
Sbjct: 65  GNLATCWSNIKEEEALERYKLITGNTVLFPEFQVYGKKDPEDNWLAASPDGIVDSLVYGL 124

Query: 163 PSQGVLEIKCPFFNGDMRNASPWSRVPLYCIPQAQGLMEIMDRDWMDFYVWTPNGSSLFR 222
            S+GVLEIKCPFFNGD   ASPWSRVPLY IPQAQGLMEIMDRDWMDFYVWT NGSSLFR
Sbjct: 125 HSRGVLEIKCPFFNGDKSIASPWSRVPLYYIPQAQGLMEIMDRDWMDFYVWTLNGSSLFR 184

Query: 223 LYRDVEYWDVLKIALSDFWWKHVQPAREMCSKYVVTNPLVELKSLRPSPRHELCSYIVCE 282
           LYRD EYWDVLKIALSDFW+KHV PARE+C K+ + +PL EL+SL+P PRHELC YIV E
Sbjct: 185 LYRDAEYWDVLKIALSDFWFKHVLPARELCRKHAINSPLTELRSLKPEPRHELCRYIVYE 244

Query: 283 SRRVVNNSKLLLREFDGRLQ 302
           S+R+V++SKLL+RE  G+LQ
Sbjct: 245 SKRIVDDSKLLMREIHGKLQ 264

[Close]

BLAST of CSPI07G12660.1 vs. TrEMBL
Match: V4SS59_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10031987mg PE=4 SV=1)

HSP 1 Score: 453.0 bits (1164), Expect = 2.9e-124
Identity = 199/275 (72.36%), Postives = 240/275 (87.27%), Query Frame = 1


Query: 27  SIRNFNSVSSSSLQFETVNHYSVLQSTSFQHWFKNWQELRKHKLTASTFAGAIGFWPRRR 86
           S ++F+S   +S  F +   +S+LQS+S QHWFKNWQ+LRKHKLTASTFAGAIGFWPRRR
Sbjct: 70  SSKSFSSYIGNSPSFLSGESHSILQSSSLQHWFKNWQKLRKHKLTASTFAGAIGFWPRRR 129

Query: 87  TQLWLEKLGAIDQFCGNLATCWSNMKEEEALERYKLITGNSVLFPEFQVYGKANSEDDWL 146
            QLWLEK+GAI+ F GNLATCWSN+KEE+ALERYKLITGN+V F EFQVY   + +DDWL
Sbjct: 130 VQLWLEKIGAIEPFLGNLATCWSNIKEEDALERYKLITGNTVYFTEFQVYRDKSRDDDWL 189

Query: 147 AASPDGAIDKMIYGLPSQGVLEIKCPFFNGDMRNASPWSRVPLYCIPQAQGLMEIMDRDW 206
           AASPDG +D+ +Y LPS GVLEIKCPFFNG++  ASPW R+PLY IPQAQGLMEI+DRDW
Sbjct: 190 AASPDGLVDRFVYDLPSGGVLEIKCPFFNGELTRASPWKRIPLYYIPQAQGLMEILDRDW 249

Query: 207 MDFYVWTPNGSSLFRLYRDVEYWDVLKIALSDFWWKHVQPAREMCSKYVVTNPLVELKSL 266
           MDFYVWTPNGSSLFR+YRD+EYWDV+K+ALSDFWWKHVQPAREMCSKYV+++PL +LK L
Sbjct: 250 MDFYVWTPNGSSLFRVYRDLEYWDVMKMALSDFWWKHVQPAREMCSKYVISDPLKQLKPL 309

Query: 267 RPSPRHELCSYIVCESRRVVNNSKLLLREFDGRLQ 302
           +P+PRHEL SY+V +S+R+++NS LL+RE  G+LQ
Sbjct: 310 KPTPRHELWSYLVYDSKRIIDNSSLLVREIGGKLQ 344

[Close]

BLAST of CSPI07G12660.1 vs. TAIR10
Match: AT1G13810.1 (AT1G13810.1 Restriction endonuclease, type II-like superfamily protein)

HSP 1 Score: 277.7 bits (709), Expect = 8.4e-75
Identity = 129/254 (50.79%), Postives = 166/254 (65.35%), Query Frame = 1


Query: 48  SVLQSTSFQHWFKNWQELRKHKLTASTFAGAIGFWPRRRTQLWLEKLGAIDQFCGNLATC 107
           +V+ +    HW KNW++LRK++LTAS FA AIGF P  R  LWLEK+GA   F GN AT 
Sbjct: 48  AVVVTEPTHHWRKNWEDLRKNRLTASNFARAIGFSPDGRRNLWLEKIGAAKPFAGNRATF 107

Query: 108 WSNMKEEEALERYKLITGNSVLFPEFQVYGKANS-EDDWLAASPDGAIDKMIYGLPSQGV 167
           W    E EALERY  +TGN +L PEF VY    S E++WL ASPDG I+ +  G+ S GV
Sbjct: 108 WDIENEVEALERYNELTGNEILIPEFVVYKNGESPEENWLGASPDGVINVVKDGVTSCGV 167

Query: 168 LEIKCPFFNGDMRNASPWSRVPLYCIPQAQGLMEIMDRDWMDFYVWTPNGSSLFRLYRDV 227
           LE+KCPF N D     PW +VP  C+PQ QGLMEI+D DW+D Y WT NGSSLFR++RD 
Sbjct: 168 LEVKCPFDNRDNSKVYPWKKVPYNCVPQLQGLMEIVDTDWLDLYCWTRNGSSLFRVWRDT 227

Query: 228 EYWDVLKIALSDFWWKHVQPAREMCSKYVVTNPLVELKSLRPSPRHELCSYIVCESRRVV 287
            +W+ +K AL DFW  HV PARE+ + + + +P V+L+  +P   HE C  I+  + R+ 
Sbjct: 228 AFWEDMKPALFDFWQNHVLPAREIYNNFDIKDPQVKLREFKPKHWHEDCKKIMRGAERIS 287

Query: 288 NNSKLLLREFDGRL 301
            N+  L  E DG L
Sbjct: 288 ANANRLFYEIDGNL 301

[Close]

BLAST of CSPI07G12660.1 vs. TAIR10
Match: AT1G67660.1 (AT1G67660.1 Restriction endonuclease, type II-like superfamily protein)

HSP 1 Score: 188.3 bits (477), Expect = 6.7e-48
Identity = 106/287 (36.93%), Postives = 150/287 (52.26%), Query Frame = 1


Query: 24  GNCSIRNFNSVSSSSLQFETVNHY----------SVLQSTSFQHWFKNWQELRKHKLTAS 83
           G+ S R   S ++ SL  +T++ +          S+L  +      + W  LRK KLT S
Sbjct: 73  GSVSSRKRFSSTALSLITQTISPFAHPRSSVIVSSLLSPSDIPQKSEEWFALRKDKLTTS 132

Query: 84  TFAGAIGFWP-RRRTQLWLEKLGAID----QFCGNLATCWSNMKEEEALERYKLITGNSV 143
           TF+ A+GFW   RR +LW EK+   D    +     A  W    E  A+ERYK I G  V
Sbjct: 133 TFSTALGFWKGNRRAELWHEKVYDSDARVVEESARFAMNWGVQMESSAIERYKRIMGCEV 192

Query: 144 LFPEFQVYGKANSEDDWLAASPDGAIDKMIYGLPSQGVLEIKCPFFNGDMRNASPWSRVP 203
               F ++  +N E  WL ASPDG +D         G+LE+KCP+  G      PW +VP
Sbjct: 193 GTMGFAIH--SNEEFHWLGASPDGILDCF-------GILEVKCPYNKGKTETVLPWKKVP 252

Query: 204 LYCIPQAQGLMEIMDRDWMDFYVWTPNGSSLFRLYRDVEYWDVLKIALSDFWWKHVQPAR 263
            Y +PQ QG MEIMDR+W++ Y WT NGS++FR+ RD  YW ++   L +FWW+ V PAR
Sbjct: 253 YYYMPQLQGQMEIMDREWVNLYCWTRNGSTVFRVMRDRSYWRIIHDVLREFWWESVIPAR 312

Query: 264 EMCSKYVVTNPLVELKSLRPSPRHELCSYIVCESRRVVNNSKLLLRE 296
           E     ++     E+K   P+  H+     + +S  +   SKL+ RE
Sbjct: 313 E---ALLLGKEDEEVKKYEPTSTHKRTKLAIAKSLNLAAESKLVCRE 347

[Close]

BLAST of CSPI07G12660.1 vs. NCBI nr
Match: gi|700189240|gb|KGN44473.1| (hypothetical protein Csa_7G302370 [Cucumis sativus])

HSP 1 Score: 641.7 bits (1654), Expect = 6.3e-181
Identity = 298/302 (98.68%), Postives = 302/302 (100.00%), Query Frame = 1


Query: 1   MGEAEKQSMFNCKKILFACSQAIGNCSIRNFNSVSSSSLQFETVNHYSVLQSTSFQHWFK 60
           MGEAEKQSMFNCKKILFACSQAIGNCSIRNFNSVSSSSLQFETVNHYSVLQSTSFQHWFK
Sbjct: 1   MGEAEKQSMFNCKKILFACSQAIGNCSIRNFNSVSSSSLQFETVNHYSVLQSTSFQHWFK 60

Query: 61  NWQELRKHKLTASTFAGAIGFWPRRRTQLWLEKLGAIDQFCGNLATCWSNMKEEEALERY 120
           NWQELRKHKLTASTFAGAIGFWPRRRTQLWLEKLGAIDQFCGNLATCWSNMKEEEALERY
Sbjct: 61  NWQELRKHKLTASTFAGAIGFWPRRRTQLWLEKLGAIDQFCGNLATCWSNMKEEEALERY 120

Query: 121 KLITGNSVLFPEFQVYGKANSEDDWLAASPDGAIDKMIYGLPSQGVLEIKCPFFNGDMRN 180
           KLITGNSVLFPEFQVYGKANSEDDWLAASPDGAIDKM+YGLPS+GVLEIKCPFFNGDMRN
Sbjct: 121 KLITGNSVLFPEFQVYGKANSEDDWLAASPDGAIDKMVYGLPSRGVLEIKCPFFNGDMRN 180

Query: 181 ASPWSRVPLYCIPQAQGLMEIMDRDWMDFYVWTPNGSSLFRLYRDVEYWDVLKIALSDFW 240
           ASPWSRVPLYCIPQAQGLMEIMDRDWMDFYVWTPNGSSLFRLYRDVEYWDVLKIALSDFW
Sbjct: 181 ASPWSRVPLYCIPQAQGLMEIMDRDWMDFYVWTPNGSSLFRLYRDVEYWDVLKIALSDFW 240

Query: 241 WKHVQPAREMCSKYVVTNPLVELKSLRPSPRHELCSYIVCESRRVVNNSKLLLREFDGRL 300
           WKHVQPAREMCSKYVVTNPL+ELKSLRPSPRHELCSYIVCES+RVVNNSKLLLREFDGRL
Sbjct: 241 WKHVQPAREMCSKYVVTNPLIELKSLRPSPRHELCSYIVCESKRVVNNSKLLLREFDGRL 300

Query: 301 QT 303
           QT
Sbjct: 301 QT 302

[Close]

BLAST of CSPI07G12660.1 vs. NCBI nr
Match: gi|778726529|ref|XP_011659114.1| (PREDICTED: uncharacterized protein LOC101215512 [Cucumis sativus])

HSP 1 Score: 626.3 bits (1614), Expect = 2.7e-176
Identity = 290/294 (98.64%), Postives = 294/294 (100.00%), Query Frame = 1


Query: 9   MFNCKKILFACSQAIGNCSIRNFNSVSSSSLQFETVNHYSVLQSTSFQHWFKNWQELRKH 68
           MFNCKKILFACSQAIGNCSIRNFNSVSSSSLQFETVNHYSVLQSTSFQHWFKNWQELRKH
Sbjct: 1   MFNCKKILFACSQAIGNCSIRNFNSVSSSSLQFETVNHYSVLQSTSFQHWFKNWQELRKH 60

Query: 69  KLTASTFAGAIGFWPRRRTQLWLEKLGAIDQFCGNLATCWSNMKEEEALERYKLITGNSV 128
           KLTASTFAGAIGFWPRRRTQLWLEKLGAIDQFCGNLATCWSNMKEEEALERYKLITGNSV
Sbjct: 61  KLTASTFAGAIGFWPRRRTQLWLEKLGAIDQFCGNLATCWSNMKEEEALERYKLITGNSV 120

Query: 129 LFPEFQVYGKANSEDDWLAASPDGAIDKMIYGLPSQGVLEIKCPFFNGDMRNASPWSRVP 188
           LFPEFQVYGKANSEDDWLAASPDGAIDKM+YGLPS+GVLEIKCPFFNGDMRNASPWSRVP
Sbjct: 121 LFPEFQVYGKANSEDDWLAASPDGAIDKMVYGLPSRGVLEIKCPFFNGDMRNASPWSRVP 180

Query: 189 LYCIPQAQGLMEIMDRDWMDFYVWTPNGSSLFRLYRDVEYWDVLKIALSDFWWKHVQPAR 248
           LYCIPQAQGLMEIMDRDWMDFYVWTPNGSSLFRLYRDVEYWDVLKIALSDFWWKHVQPAR
Sbjct: 181 LYCIPQAQGLMEIMDRDWMDFYVWTPNGSSLFRLYRDVEYWDVLKIALSDFWWKHVQPAR 240

Query: 249 EMCSKYVVTNPLVELKSLRPSPRHELCSYIVCESRRVVNNSKLLLREFDGRLQT 303
           EMCSKYVVTNPL+ELKSLRPSPRHELCSYIVCES+RVVNNSKLLLREFDGRLQT
Sbjct: 241 EMCSKYVVTNPLIELKSLRPSPRHELCSYIVCESKRVVNNSKLLLREFDGRLQT 294

[Close]

BLAST of CSPI07G12660.1 vs. NCBI nr
Match: gi|700192625|gb|KGN47829.1| (hypothetical protein Csa_6G405910 [Cucumis sativus])

HSP 1 Score: 607.1 bits (1564), Expect = 1.7e-170
Identity = 281/294 (95.58%), Postives = 288/294 (97.96%), Query Frame = 1


Query: 9   MFNCKKILFACSQAIGNCSIRNFNSVSSSSLQFETVNHYSVLQSTSFQHWFKNWQELRKH 68
           MFN KKILFACSQAIGNCS+RNFNSVS SSLQFET NHYSVLQS+SFQHWFKNWQELRKH
Sbjct: 1   MFNSKKILFACSQAIGNCSLRNFNSVSFSSLQFETGNHYSVLQSSSFQHWFKNWQELRKH 60

Query: 69  KLTASTFAGAIGFWPRRRTQLWLEKLGAIDQFCGNLATCWSNMKEEEALERYKLITGNSV 128
           KLTASTFAGAIGFWPRRRTQLWLEKLGAIDQFCGNLATCWSNMKEEEALERYKLITGNSV
Sbjct: 61  KLTASTFAGAIGFWPRRRTQLWLEKLGAIDQFCGNLATCWSNMKEEEALERYKLITGNSV 120

Query: 129 LFPEFQVYGKANSEDDWLAASPDGAIDKMIYGLPSQGVLEIKCPFFNGDMRNASPWSRVP 188
           LFPEFQVYGKANSEDDWLAASPDGAIDKM+YGLPS+GVLEIKCPFFNGD+RNA PWSRVP
Sbjct: 121 LFPEFQVYGKANSEDDWLAASPDGAIDKMVYGLPSRGVLEIKCPFFNGDLRNALPWSRVP 180

Query: 189 LYCIPQAQGLMEIMDRDWMDFYVWTPNGSSLFRLYRDVEYWDVLKIALSDFWWKHVQPAR 248
            YCIPQAQGLMEIMDRDWMDFYVWTPNGSSLFRLYRD EYWDVLKIALSDFWWKHVQPAR
Sbjct: 181 RYCIPQAQGLMEIMDRDWMDFYVWTPNGSSLFRLYRDPEYWDVLKIALSDFWWKHVQPAR 240

Query: 249 EMCSKYVVTNPLVELKSLRPSPRHELCSYIVCESRRVVNNSKLLLREFDGRLQT 303
           EMCSKYV+TNPLVELKSLRPSPRHELCSYIVCES+RVVNNSKLLLREFDGRLQT
Sbjct: 241 EMCSKYVITNPLVELKSLRPSPRHELCSYIVCESKRVVNNSKLLLREFDGRLQT 294

[Close]

BLAST of CSPI07G12660.1 vs. NCBI nr
Match: gi|659113272|ref|XP_008456487.1| (PREDICTED: uncharacterized protein LOC103496427 [Cucumis melo])

HSP 1 Score: 601.7 bits (1550), Expect = 7.2e-169
Identity = 278/294 (94.56%), Postives = 286/294 (97.28%), Query Frame = 1


Query: 9   MFNCKKILFACSQAIGNCSIRNFNSVSSSSLQFETVNHYSVLQSTSFQHWFKNWQELRKH 68
           MFN KKILFACSQAIGNCSIRNFNSVS SSLQFET NHYSVLQS+SFQHWFKNWQELRKH
Sbjct: 1   MFNSKKILFACSQAIGNCSIRNFNSVSFSSLQFETGNHYSVLQSSSFQHWFKNWQELRKH 60

Query: 69  KLTASTFAGAIGFWPRRRTQLWLEKLGAIDQFCGNLATCWSNMKEEEALERYKLITGNSV 128
           KLTASTFAGAIGFWPRRR QLWLEKLGAI+ FCGNLATCWSNMKEEEALERYKLITGNSV
Sbjct: 61  KLTASTFAGAIGFWPRRRAQLWLEKLGAIEPFCGNLATCWSNMKEEEALERYKLITGNSV 120

Query: 129 LFPEFQVYGKANSEDDWLAASPDGAIDKMIYGLPSQGVLEIKCPFFNGDMRNASPWSRVP 188
           LFPEFQVYGKANSEDDWLAASPDGAIDKM+YGLPS+GVLEIKCPFFNGDMRNASPWS+VP
Sbjct: 121 LFPEFQVYGKANSEDDWLAASPDGAIDKMVYGLPSRGVLEIKCPFFNGDMRNASPWSQVP 180

Query: 189 LYCIPQAQGLMEIMDRDWMDFYVWTPNGSSLFRLYRDVEYWDVLKIALSDFWWKHVQPAR 248
            YCIPQAQGLMEIMDRDWMDFYVWTPNGSSLFRLYRD EYWDVLKIALSDFWWKHVQPAR
Sbjct: 181 RYCIPQAQGLMEIMDRDWMDFYVWTPNGSSLFRLYRDPEYWDVLKIALSDFWWKHVQPAR 240

Query: 249 EMCSKYVVTNPLVELKSLRPSPRHELCSYIVCESRRVVNNSKLLLREFDGRLQT 303
           E+CSKYV+TNPL+ELKS RPSPRHELCSYIVCESRRVVNNSKLLLREFDGRLQT
Sbjct: 241 EICSKYVITNPLIELKSFRPSPRHELCSYIVCESRRVVNNSKLLLREFDGRLQT 294

[Close]

BLAST of CSPI07G12660.1 vs. NCBI nr
Match: gi|778722290|ref|XP_004149919.2| (PREDICTED: uncharacterized protein LOC101207616 [Cucumis sativus])

HSP 1 Score: 537.0 bits (1382), Expect = 2.2e-149
Identity = 243/258 (94.19%), Postives = 253/258 (98.06%), Query Frame = 1


Query: 45  NHYSVLQSTSFQHWFKNWQELRKHKLTASTFAGAIGFWPRRRTQLWLEKLGAIDQFCGNL 104
           +H ++ +S+SFQHWFKNWQELRKHKLTASTFAGAIGFWPRRRTQLWLEKLGAIDQFCGNL
Sbjct: 8   SHPAIFKSSSFQHWFKNWQELRKHKLTASTFAGAIGFWPRRRTQLWLEKLGAIDQFCGNL 67

Query: 105 ATCWSNMKEEEALERYKLITGNSVLFPEFQVYGKANSEDDWLAASPDGAIDKMIYGLPSQ 164
           ATCWSNMKEEEALERYKLITGNSVLFPEFQVYGKANSEDDWLAASPDGAIDKM+YGLPS+
Sbjct: 68  ATCWSNMKEEEALERYKLITGNSVLFPEFQVYGKANSEDDWLAASPDGAIDKMVYGLPSR 127

Query: 165 GVLEIKCPFFNGDMRNASPWSRVPLYCIPQAQGLMEIMDRDWMDFYVWTPNGSSLFRLYR 224
           GVLEIKCPFFNGD+RNA PWSRVP YCIPQAQGLMEIMDRDWMDFYVWTPNGSSLFRLYR
Sbjct: 128 GVLEIKCPFFNGDLRNALPWSRVPRYCIPQAQGLMEIMDRDWMDFYVWTPNGSSLFRLYR 187

Query: 225 DVEYWDVLKIALSDFWWKHVQPAREMCSKYVVTNPLVELKSLRPSPRHELCSYIVCESRR 284
           D EYWDVLKIALSDFWWKHVQPAREMCSKYV+TNPLVELKSLRPSPRHELCSYIVCES+R
Sbjct: 188 DPEYWDVLKIALSDFWWKHVQPAREMCSKYVITNPLVELKSLRPSPRHELCSYIVCESKR 247

Query: 285 VVNNSKLLLREFDGRLQT 303
           VVNNSKLLLREFDGRLQT
Sbjct: 248 VVNNSKLLLREFDGRLQT 265

The following BLAST results are available for this feature:

BLAST of CSPI07G12660.1 vs. Swiss-Prot


Match Name	E-value	Identity	Description

BLAST of CSPI07G12660.1 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K4T5_CUCSA	4.4e-181	98.68	Uncharacterized protein OS=Cucumis sativus GN=Csa_7G302370 PE=4 SV=1	[more]
A0A0A0KG47_CUCSA	1.2e-170	95.58	Uncharacterized protein OS=Cucumis sativus GN=Csa_6G405910 PE=4 SV=1	[more]
A0A061F0P2_THECC	1.8e-126	79.61	DNA-directed RNA polymerase subunit beta OS=Theobroma cacao GN=TCM_025578 PE=4 S...	[more]
F6I2K8_VITVI	2.2e-124	78.85	Putative uncharacterized protein OS=Vitis vinifera GN=VIT_16s0013g00380 PE=4 SV=...	[more]
V4SS59_9ROSI	2.9e-124	72.36	Uncharacterized protein OS=Citrus clementina GN=CICLE_v10031987mg PE=4 SV=1	[more]

BLAST of CSPI07G12660.1 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G13810.1	8.4e-75	50.79	Restriction endonuclease, type II-like superfamily protein	[more]
AT1G67660.1	6.7e-48	36.93	Restriction endonuclease, type II-like superfamily protein	[more]

BLAST of CSPI07G12660.1 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700189240\|gb\|KGN44473.1\|	6.3e-181	98.68	hypothetical protein Csa_7G302370 [Cucumis sativus]	[more]
gi\|778726529\|ref\|XP_011659114.1\|	2.7e-176	98.64	PREDICTED: uncharacterized protein LOC101215512 [Cucumis sativus]	[more]
gi\|700192625\|gb\|KGN47829.1\|	1.7e-170	95.58	hypothetical protein Csa_6G405910 [Cucumis sativus]	[more]
gi\|659113272\|ref\|XP_008456487.1\|	7.2e-169	94.56	PREDICTED: uncharacterized protein LOC103496427 [Cucumis melo]	[more]
gi\|778722290\|ref\|XP_004149919.2\|	2.2e-149	94.19	PREDICTED: uncharacterized protein LOC101207616 [Cucumis sativus]	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **INTERPRO**
Term	Definition
IPR011335	Restrct_endonuc-II-like
IPR011604	Exonuc_phg/RecB_C
IPR019080	YqaJ_viral_recombinase

Vocabulary: **Molecular Function**
Term	Definition
GO:0003677	DNA binding
GO:0004518	nuclease activity

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0090305	nucleic acid phosphodiester bond hydrolysis
biological_process	GO:0006144	purine nucleobase metabolic process
biological_process	GO:0006206	pyrimidine nucleobase metabolic process
biological_process	GO:0006351	transcription, DNA-templated
cellular_component	GO:0005730	nucleolus
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0004518	nuclease activity
molecular_function	GO:0003899	DNA-directed RNA polymerase activity

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CSPI07G12660	CSPI07G12660	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CSPI07G12660.1	CSPI07G12660.1-protein	polypeptide

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI07G12660.1.cds2	CSPI07G12660.1.cds2	CDS
CSPI07G12660.1.cds1	CSPI07G12660.1.cds1	CDS

InterPro

Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR011335	Restriction endonuclease type II-like	unknown	SSF52980	Restriction endonuclease-like	coord: 46..250 score: 1.5
IPR011604	Exonuclease, phage-type/RecB, C-terminal	GENE3D	G3DSA:3.90.320.10		coord: 58..250 score: 5.2
IPR019080	YqaJ viral recombinase	PFAM	PF09588	YqaJ	coord: 62..204 score: 1.9