CSPI06G07680.1 (mRNA) Wild cucumber (PI 183967) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CSPI06G07680.1
Type	mRNA
Organism	Cucumis sativus (Wild cucumber (PI 183967))
Description	Heavy metal transport/detoxification superfamily protein
Location	Chr6 : 6490577 .. 6491743 (-)
Sequence length	756

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATGCCATTACCCTCTCATCACACTTCTTTTTTCAACAGTGGGATTTCAAATCATACAAAGAGATGGGAAAGGTATGGGAAAAAAAAACCCTTAATTTTCATAACTTAATATATGTTTAGGTTGCAATTTATTATATACAATATTATTGATTTTTTTTTTTATTTAATTGAAGGGTCAAAATCAAAATGGGAAGAGAAGGCAAGAAGAAGAGAAGGGAAAGGGAAAAAAAGAACAGAAAAAGATAACAGAAGTTGTGGTTAAAGTTTATATGCATTGTGAAGGCTGCAAACATAAACTCTCTAAATGCTTGAAAGCTTCTCGCCATGGTAACTGACCTATTTTATTCTTCACTTTTTTTCTTTGTTAAACTACTGTAAATATGTTGCTACCGATCTTATGTTATTATATTTTTACAATGTTTGGGTTATATATATATATATAGGTGTGGAGGAAGTGGCAGCTGATATGGAAAATAAAAAAGTAGTAATAAAAGGAAACGATCTAAACCCAGAGAAAATAATGGAAGAAATTAAGAAAAAATACTCAAAAAATGTGGAACTTATTTCTCCTAAGGAACTGCCTGATACTTCCAAACAAAACAAACCTGAACCTAAAAAGGAAGAGGTAAATTCTAAATTTTTATTTATTTTAAATATTCAACTTCCTTAATTATGCATTGAATGATTGGGTAATTTGTAGGTTCAAATGAAGACAGTGGAACTAAAAATGAATATGCATTGTGAAGGCTGTGAAAACGACATCAAACAAACCATTGGAAAAATGGAAGGTAAGAAATGAATAATGCACTATATATGTATATACTAATGTAAAATTTGTAGTTTAATTAAACAACATATTAAATAAGTACCATTGTTTTGTATATGAGAATTATGAATATTGATGTAGGAGTAATGAGAGTGGAAACAGATAGAGAGAGTTCAAAAGTGATAGTGAAGGGAATGATTGATCCTCCAAAGCTAATGGAATGCATTAAAAAGAGAATGGGAAAGAAGGTTGAGATTTTTAATAAGAAATCTGAAGACAAATCAAGCAACAACAAAGATTCAAATGTTCAACACAAGTTGGATCATTTTACATTTAAATATCCTCCTCAATATTCCTTGCTTCATATTTACCCCAATCAAACTTTTAGTGATGATAAT

mRNA sequence

ATGGATGCCATTACCCTCTCATCACACTTCTTTTTTCAACAGTGGGATTTCAAATCATACAAAGAGATGGGAAAGGGTCAAAATCAAAATGGGAAGAGAAGGCAAGAAGAAGAGAAGGGAAAGGGAAAAAAAGAACAGAAAAAGATAACAGAAGTTGTGGTTAAAGTTTATATGCATTGTGAAGGCTGCAAACATAAACTCTCTAAATGCTTGAAAGCTTCTCGCCATGGTGTGGAGGAAGTGGCAGCTGATATGGAAAATAAAAAAGTAGTAATAAAAGGAAACGATCTAAACCCAGAGAAAATAATGGAAGAAATTAAGAAAAAATACTCAAAAAATGTGGAACTTATTTCTCCTAAGGAACTGCCTGATACTTCCAAACAAAACAAACCTGAACCTAAAAAGGAAGAGGTTCAAATGAAGACAGTGGAACTAAAAATGAATATGCATTGTGAAGGCTGTGAAAACGACATCAAACAAACCATTGGAAAAATGGAAGGAGTAATGAGAGTGGAAACAGATAGAGAGAGTTCAAAAGTGATAGTGAAGGGAATGATTGATCCTCCAAAGCTAATGGAATGCATTAAAAAGAGAATGGGAAAGAAGGTTGAGATTTTTAATAAGAAATCTGAAGACAAATCAAGCAACAACAAAGATTCAAATGTTCAACACAAGTTGGATCATTTTACATTTAAATATCCTCCTCAATATTCCTTGCTTCATATTTACCCCAATCAAACTTTTAGTGATGATAAT

Coding sequence (CDS)

ATGGATGCCATTACCCTCTCATCACACTTCTTTTTTCAACAGTGGGATTTCAAATCATACAAAGAGATGGGAAAGGGTCAAAATCAAAATGGGAAGAGAAGGCAAGAAGAAGAGAAGGGAAAGGGAAAAAAAGAACAGAAAAAGATAACAGAAGTTGTGGTTAAAGTTTATATGCATTGTGAAGGCTGCAAACATAAACTCTCTAAATGCTTGAAAGCTTCTCGCCATGGTGTGGAGGAAGTGGCAGCTGATATGGAAAATAAAAAAGTAGTAATAAAAGGAAACGATCTAAACCCAGAGAAAATAATGGAAGAAATTAAGAAAAAATACTCAAAAAATGTGGAACTTATTTCTCCTAAGGAACTGCCTGATACTTCCAAACAAAACAAACCTGAACCTAAAAAGGAAGAGGTTCAAATGAAGACAGTGGAACTAAAAATGAATATGCATTGTGAAGGCTGTGAAAACGACATCAAACAAACCATTGGAAAAATGGAAGGAGTAATGAGAGTGGAAACAGATAGAGAGAGTTCAAAAGTGATAGTGAAGGGAATGATTGATCCTCCAAAGCTAATGGAATGCATTAAAAAGAGAATGGGAAAGAAGGTTGAGATTTTTAATAAGAAATCTGAAGACAAATCAAGCAACAACAAAGATTCAAATGTTCAACACAAGTTGGATCATTTTACATTTAAATATCCTCCTCAATATTCCTTGCTTCATATTTACCCCAATCAAACTTTTAGTGATGATAAT

Homology

[Close]

BLAST of CSPI06G07680.1 vs. Swiss-Prot
Match: HIP3_ARATH (Heavy metal-associated isoprenylated plant protein 3 OS=Arabidopsis thaliana GN=HIPP3 PE=1 SV=1)

HSP 1 Score: 98.2 bits (243), Expect = 1.4e-19
Identity = 73/216 (33.80%), Postives = 123/216 (56.94%), Query Frame = 1


Query: 23  MGKGQNQNGKRRQEEEKGKGKKEQKKITEVVVKVYMHCEGCKHKLSKCLKASRHGVEEVA 82
           MG+ +N+ G  +++    K K E   IT VV+KV MHCEGC  ++ KC++ S  GVE V 
Sbjct: 1   MGEKKNE-GDNKKKGGDNKKKNETPSIT-VVLKVDMHCEGCASRIVKCVR-SFQGVETVK 60

Query: 83  ADMENKKVVIKGNDLNPEKIMEEIKKKYSKNVELISPKELPDTSKQNKP----------- 142
           ++    K+ + G  L+P K+ E++++K  K V+L+SP+   +  K+NK            
Sbjct: 61  SESATGKLTVTGA-LDPVKLREKLEEKTKKKVDLVSPQPKKEKEKENKNKNDEDKKKSEE 120

Query: 143 -------EPKKEEVQMKTVELKMNMHCEGCENDIKQTIGKMEGVMRVETDRESSKVIVKG 202
                  + K +E  + T  LK+N HC+GC   I++T+ K +GV  +  D+E + + VKG
Sbjct: 121 KKKPDNNDKKPKETPVTTAVLKLNFHCQGCIGKIQKTVTKTKGVNGLTMDKEKNLLTVKG 180

Query: 203 MIDPPKLMECIKKRMGKKVEIF-NKKSEDKSSNNKD 220
            +D  KL+E + +++ + VEI   KK +DK + N++
Sbjct: 181 TMDVKKLVEILSEKLKRAVEIVPPKKEKDKENGNEN 212

[Close]

BLAST of CSPI06G07680.1 vs. Swiss-Prot
Match: HIP26_ARATH (Heavy metal-associated isoprenylated plant protein 26 OS=Arabidopsis thaliana GN=HIPP26 PE=1 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 7.0e-08
Identity = 33/86 (38.37%), Postives = 56/86 (65.12%), Query Frame = 1


Query: 121 ELPDTSKQNKPEPKKEEVQMKTVELKMNMHCEGCENDIKQTIGKMEGVMRVETDRESSKV 180
           E+ D S  +K + +K   Q++TVE+K+ M CEGCE  +++++  M+GV  V  + ++ KV
Sbjct: 9   EMFDCSHGHKIKKRK---QLQTVEIKVKMDCEGCERKVRRSVEGMKGVSSVTLEPKAHKV 68

Query: 181 IVKGMIDPPKLMECIKKRMGKKVEIF 207
            V G +DP K++  +  R GKKVE++
Sbjct: 69  TVVGYVDPNKVVARMSHRTGKKVELW 91

[Close]

BLAST of CSPI06G07680.1 vs. Swiss-Prot
Match: HIP27_ARATH (Heavy metal-associated isoprenylated plant protein 27 OS=Arabidopsis thaliana GN=HIPP27 PE=1 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 7.0e-08
Identity = 27/72 (37.50%), Postives = 47/72 (65.28%), Query Frame = 1


Query: 135 KEEVQMKTVELKMNMHCEGCENDIKQTIGKMEGVMRVETDRESSKVIVKGMIDPPKLMEC 194
           K+  Q + VE+K+ M CEGCE  +++++  M+GV +V  D + SK+ V+G + P K++  
Sbjct: 13  KKLKQFQKVEIKVKMDCEGCERRVRKSVEGMKGVSKVTVDPKQSKLTVEGFVQPSKVVHR 72

Query: 195 IKKRMGKKVEIF 207
           +  R GKK E++
Sbjct: 73  VMHRTGKKAELW 84

[Close]

BLAST of CSPI06G07680.1 vs. Swiss-Prot
Match: HIP20_ARATH (Heavy metal-associated isoprenylated plant protein 20 OS=Arabidopsis thaliana GN=HIPP20 PE=2 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 7.0e-08
Identity = 29/73 (39.73%), Postives = 48/73 (65.75%), Query Frame = 1


Query: 134 KKEEVQMKTVELKMNMHCEGCENDIKQTIGKMEGVMRVETDRESSKVIVKGMIDPPKLME 193
           +++   M+TV +K+ M C+GCE  +K  +  M+GV  VE +R+  KV V G ++P K+++
Sbjct: 21  RRKRKVMQTVNIKVKMDCDGCERRVKNAVSSMKGVKSVEVNRKIHKVTVSGYVEPKKVLK 80

Query: 194 CIKKRMGKKVEIF 207
            I +R GKK EI+
Sbjct: 81  RI-ERTGKKAEIW 92

[Close]

BLAST of CSPI06G07680.1 vs. Swiss-Prot
Match: HIP21_ARATH (Heavy metal-associated isoprenylated plant protein 21 OS=Arabidopsis thaliana GN=HIPP21 PE=2 SV=1)

HSP 1 Score: 57.4 bits (137), Expect = 2.6e-07
Identity = 26/73 (35.62%), Postives = 48/73 (65.75%), Query Frame = 1


Query: 134 KKEEVQMKTVELKMNMHCEGCENDIKQTIGKMEGVMRVETDRESSKVIVKGMIDPPKLME 193
           K +   ++TV++K+ M C+GCE  ++  + +M+GV  VE +R+ S++ V G +DP K+++
Sbjct: 19  KTKRKPLQTVDIKVKMDCDGCERRVRNVVRRMKGVKSVEVNRKQSRITVNGHVDPNKVLK 78

Query: 194 CIKKRMGKKVEIF 207
            +K   GKK E +
Sbjct: 79  RVKS-TGKKAEFW 90

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BLAST of CSPI06G07680.1 vs. TrEMBL
Match: A0A0A0KFC0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G094150 PE=4 SV=1)

HSP 1 Score: 486.9 bits (1252), Expect = 1.5e-134
Identity = 251/253 (99.21%), Postives = 252/253 (99.60%), Query Frame = 1


Query: 1   MDAITLSSHFFFQQWDFKSYKEMGKGQNQNGKRRQEEEKGKGKK-EQKKITEVVVKVYMH 60
           MDAITLSSHFFFQQWDFKSYKEMGKGQNQNGKRRQEEEKGKGKK EQKKITEVVVKVYMH
Sbjct: 1   MDAITLSSHFFFQQWDFKSYKEMGKGQNQNGKRRQEEEKGKGKKEEQKKITEVVVKVYMH 60

Query: 61  CEGCKHKLSKCLKASRHGVEEVAADMENKKVVIKGNDLNPEKIMEEIKKKYSKNVELISP 120
           CEGCKHKLSKCLKASRHGVEEVAADMENKKVVIKGNDLNPE+IMEEIKKKYSKNVELISP
Sbjct: 61  CEGCKHKLSKCLKASRHGVEEVAADMENKKVVIKGNDLNPERIMEEIKKKYSKNVELISP 120

Query: 121 KELPDTSKQNKPEPKKEEVQMKTVELKMNMHCEGCENDIKQTIGKMEGVMRVETDRESSK 180
           KELPDTSKQNKPEPKKEEVQMKTVELKMNMHCEGCENDIKQTIGKMEGVMRVETDRESSK
Sbjct: 121 KELPDTSKQNKPEPKKEEVQMKTVELKMNMHCEGCENDIKQTIGKMEGVMRVETDRESSK 180

Query: 181 VIVKGMIDPPKLMECIKKRMGKKVEIFNKKSEDKSSNNKDSNVQHKLDHFTFKYPPQYSL 240
           VIVKGMIDPPKLMECIKKRMGKKVEIFNKKSEDKSSNNKDSNVQHKLDHFTFKYPPQYSL
Sbjct: 181 VIVKGMIDPPKLMECIKKRMGKKVEIFNKKSEDKSSNNKDSNVQHKLDHFTFKYPPQYSL 240

Query: 241 LHIYPNQTFSDDN 253
           LHIYPNQTFSDDN
Sbjct: 241 LHIYPNQTFSDDN 253

[Close]

BLAST of CSPI06G07680.1 vs. TrEMBL
Match: M5XSC8_PRUPE (Uncharacterized protein (Fragment) OS=Prunus persica GN=PRUPE_ppa022179mg PE=4 SV=1)

HSP 1 Score: 207.6 bits (527), Expect = 1.8e-50
Identity = 113/230 (49.13%), Postives = 161/230 (70.00%), Query Frame = 1


Query: 25  KGQNQNGKRRQEEEKGKGKKEQKKITEVVVKVYMHCEGCKHKLSKCLKASRHGVEEVAAD 84
           +  NQN ++ Q++EK     +     E+V++V MHCEGCK K++KCLKA   G+E+V  D
Sbjct: 6   ENNNQNAEKNQQDEKNSQSSK-----EIVLRVLMHCEGCKSKVAKCLKAVE-GIEDVTVD 65

Query: 85  MENKKVVIKGNDLNPEKIMEEIKKKYSKNVELISPKELPDTSKQNKPEPKKE-EVQMKTV 144
             N++VV+KG   NP K++E ++KKYS N ELISPK  P+  ++ +P+ K+E + Q+K V
Sbjct: 66  YANQRVVVKGKKANPLKVLERLQKKYSSNAELISPKPKPENKEKKEPQKKEESQPQVKVV 125

Query: 145 ELKMNMHCEGCENDIKQTIGKMEGVMRVETDRESSKVIVKGMIDPPKLMECIKKRMGKKV 204
            LKM MHCEGC NDIK+ + KM+GV+ VE + ESS+V V+G++DPPKL+E IKK++GK  
Sbjct: 126 VLKMLMHCEGCANDIKKYLEKMKGVLSVEANMESSRVTVRGVVDPPKLIEYIKKQLGKHA 185

Query: 205 EIFNKKSEDKSSNNKDSNVQHKLDHFT-FKYPPQYSLLHIYPNQTFSDDN 253
           EI  ++   K  NN ++N +   +    F+YPPQYS  HIYPNQTFSD+N
Sbjct: 186 EIVKQEQGQKQGNNNNNNNKKDPERENIFQYPPQYSSQHIYPNQTFSDEN 229

[Close]

BLAST of CSPI06G07680.1 vs. TrEMBL
Match: A0A061E5Z1_THECC (Heavy metal transport/detoxification superfamily protein, putative OS=Theobroma cacao GN=TCM_008612 PE=4 SV=1)

HSP 1 Score: 193.7 bits (491), Expect = 2.6e-46
Identity = 114/239 (47.70%), Postives = 150/239 (62.76%), Query Frame = 1


Query: 23  MGKGQNQNG---------KRRQEEEKGKGKKEQKKITEVVVKVYMHCEGCKHKLSKCLKA 82
           MG GQ   G         ++  E  + KG K+  K  E+V+KVYMHCEGC  K+  CLK 
Sbjct: 1   MGHGQQHQGGGQIVEVKLEKSCENNQEKGAKDSNKGKEIVLKVYMHCEGCAAKVFNCLKG 60

Query: 83  SRHGVEEVAADMENKKVVIKGNDLNPEKIMEEIKKKYSKNVELISPKELPDTSKQNKPEP 142
            + GVE+V  DME  +V++KG + +P KI+E +KKKYS+N ELISPK  P  +   K   
Sbjct: 61  FQ-GVEQVKTDMEGDRVIVKGQNADPLKILERVKKKYSRNAELISPKPKPKAT-DGKQSQ 120

Query: 143 KKEEVQMKTVELKMNMHCEGCENDIKQTIGKMEGVMRVETDRESSKVIVKGMIDPPKLME 202
            K+E  +K V LKM MHCEGC NDIK++IG+M+G++ VE D + S V V+G  DPPKL+E
Sbjct: 121 NKQEPPIKFVVLKMYMHCEGCANDIKRSIGRMKGILNVEPDMKKSTVTVRGNFDPPKLVE 180

Query: 203 CIKKRMGKKVEIFNKKSEDKSSNNKDSNVQHKLDHFTFKYPPQYSLLHIYPNQTFSDDN 253
            I K+ GK  EI  +   DK++    +  Q K +   F YPPQYSL HIYP Q FSD+N
Sbjct: 181 AIAKQFGKYAEIVAEGPTDKANG---TGKQGKEEEIMFHYPPQYSLQHIYPTQIFSDEN 234

[Close]

BLAST of CSPI06G07680.1 vs. TrEMBL
Match: A0A072UU25_MEDTR (Heavy metal transport/detoxification superfamily protein OS=Medicago truncatula GN=MTR_3g435930 PE=4 SV=1)

HSP 1 Score: 190.7 bits (483), Expect = 2.2e-45
Identity = 111/239 (46.44%), Postives = 157/239 (65.69%), Query Frame = 1


Query: 23  MGKGQNQNGKRRQEEEKGKGKKEQKKITEVVVKVYMHCEGCKHKLSKCLKASRHGVEEVA 82
           MGK   ++    +E  + KGKKE  K   VV K  +HC+GC  ++SKCLK    G+  + 
Sbjct: 1   MGKKNKEHNNNNEENSENKGKKESDKC--VVFKANIHCDGCSDQISKCLKGFE-GISHIK 60

Query: 83  ADMENKKVVIKGNDL-NPEKIMEEIKKKYSKNVELISPKELPDTSKQNKPEPKKEEVQMK 142
            D EN ++ +KG+ + +P K++E ++KK+SKNVELISPK  P+  ++ +PE KKE+ ++K
Sbjct: 61  IDRENHRIFLKGDVIKDPSKVLERLQKKFSKNVELISPKPKPENKQKKEPE-KKEQAKVK 120

Query: 143 TVELKMNMHCEGCENDIKQTIGKMEGVMRVETDRESSKVIVKGMIDPPKLMECIKKRMGK 202
           TV LKM +HCEGCE+D+K+ I KMEGV  VE D+E S V VKG ++ PKL+E +KK+ GK
Sbjct: 121 TVVLKMYIHCEGCESDVKKNIEKMEGVESVELDKEKSHVTVKGTVESPKLVEYVKKKFGK 180

Query: 203 KVEIFN--------KKSEDKSSNNKDSNVQHKLDHFTFKYPPQYSLLHIYPNQTFSDDN 253
             EI          KK + K++N+KDS   H +    F YPPQYS  ++YPNQ FSD+N
Sbjct: 181 HAEIIKDDGKRDQVKKDQGKNNNDKDSG--HVI---MFSYPPQYSTQYLYPNQNFSDEN 230

[Close]

BLAST of CSPI06G07680.1 vs. TrEMBL
Match: R0HIQ9_9BRAS (Uncharacterized protein (Fragment) OS=Capsella rubella GN=CARUB_v10015807mg PE=4 SV=1)

HSP 1 Score: 187.2 bits (474), Expect = 2.5e-44
Identity = 110/242 (45.45%), Postives = 158/242 (65.29%), Query Frame = 1


Query: 18  KSYKEMGKGQNQNGKRRQEE-EKGKGKKEQKKIT--EVVVKVYMHCEGCKHKLSKCLKAS 77
           K+ K      +++ K+ Q+  +    K +QKK    E+V+KVYMHCEGC  ++S CL+  
Sbjct: 28  KNQKNGDSSVDKSDKKNQKHGDSSVDKSDQKKTQCKEIVLKVYMHCEGCASQVSNCLRGY 87

Query: 78  RHGVEEVAADMENKKVVIKGNDLNPEKIMEEIKKKYSKNVELISPKELPDTSKQNKPEPK 137
             GVE++  ++ N KVV+ G   +P KI+  ++KK+SKN ELISPK  P   ++ +P+PK
Sbjct: 88  -DGVEQIKTEIGNDKVVVSGKFDDPMKILRRVQKKFSKNAELISPKPNPKQDQKKEPQPK 147

Query: 138 KEEV-QMKTVELKMNMHCEGCENDIKQTIGKMEGVMRVETDRESSKVIVKGMIDPPKLME 197
           KE   Q+KTV LKMNMHCEGC ++IK+ I K++GV  VE DR  S V+V+G++DPPKL+E
Sbjct: 148 KETTPQIKTVILKMNMHCEGCVHEIKRGIEKIKGVQTVEPDRAKSTVVVRGVMDPPKLVE 207

Query: 198 CIKKRMGKKVEIFNKKSEDKSSNNKDSNVQHKLD---HFTFKYPPQYSLLHIYPNQTFSD 253
            IKK++GK  E+ ++ +E    NN + N     D   +  F YPPQYS  H YP+Q FSD
Sbjct: 208 KIKKKLGKHAELASQTTEKNKDNNNNKNTNKNEDSDGNRIFSYPPQYSSQHAYPSQLFSD 267

[Close]

BLAST of CSPI06G07680.1 vs. TAIR10
Match: AT3G02960.1 (AT3G02960.1 Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 176.8 bits (447), Expect = 1.7e-44
Identity = 104/237 (43.88%), Postives = 157/237 (66.24%), Query Frame = 1


Query: 21  KEMGKGQNQNGKRRQEEEKGKGKKEQKK--ITEVVVKVYMHCEGCKHKLSKCLKASRHGV 80
           K+ G+  N++ K+ Q+       K  KK    E+V+KVYMHCEGC  ++S CL+    GV
Sbjct: 5   KQNGESDNKSEKKNQKNGDSSVDKSDKKNQCKEIVLKVYMHCEGCASQVSHCLRGY-DGV 64

Query: 81  EEVAADMENKKVVIKGNDLNPEKIMEEIKKKYSKNVELISPKELPDTSKQNKPEPKKEEV 140
           E +  ++ + KVV+ G   +P KI+  ++KK+S+N E+ISPK  P    Q +P+ KKE  
Sbjct: 65  EHIKTEIGDNKVVVSGKFDDPLKILRRVQKKFSRNAEMISPKHNPKQD-QKEPQQKKESA 124

Query: 141 -QMKTVELKMNMHCEGCENDIKQTIGKMEGVMRVETDRESSKVIVKGMIDPPKLMECIKK 200
            ++KT  L+MNMHCEGC ++IK+ I K++G+  VE DR  S V+V+G++DPPKL+E IKK
Sbjct: 125 PEIKTAILRMNMHCEGCVHEIKRGIEKIKGIQSVEPDRSKSTVVVRGVMDPPKLVEKIKK 184

Query: 201 RMGKKVEIFNKKSE-DKSSNNKDSNVQHKLD-HFTFKYPPQYSLLHIYPNQTFSDDN 253
           ++GK  E+ ++ +E  K +N K++N + + D +  F YPPQYS  H YP+Q FSD+N
Sbjct: 185 KLGKHAELLSQITEKGKDNNKKNNNKKEESDGNKIFSYPPQYSSQHAYPSQIFSDEN 239

[Close]

BLAST of CSPI06G07680.1 vs. TAIR10
Match: AT5G50740.3 (AT5G50740.3 Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 125.2 bits (313), Expect = 5.8e-29
Identity = 77/216 (35.65%), Postives = 137/216 (63.43%), Query Frame = 1


Query: 21  KEMGKGQNQNGKRRQEEEKGKGKKEQKKITEVVVKVYMHCEGCKHKLSKCLKASRHGVEE 80
           K+M + +++  + + E++K + +K+ K+  E+V+K++MHCEGC  K+ +CLK    GVE+
Sbjct: 6   KKMEEKKSEEPQAKSEDKKPEEEKK-KEPQEIVLKIFMHCEGCAKKIHRCLKGFE-GVED 65

Query: 81  VAADMENKKVVIKGNDLNPEKIMEEIKKKYSKNVELISP-------KELPDTSKQNKPEP 140
           V  D +  KVV+KG   +P K+++ +++K  + VELISP        + P+  ++ KP+P
Sbjct: 66  VTTDCKTSKVVVKGEKADPLKVLQRLQRKSHRQVELISPIPEPKPVSDEPEKKEKEKPKP 125

Query: 141 KKEEVQMKTVELKMNMHCEGCENDIKQTIGKMEGVMRVETDRESSKVIVKGMIDPPKLME 200
           ++++ ++ TV L+++MHCE C  +I++ I +M+GV  VE D ++S+V VKG+  P KL+E
Sbjct: 126 EEKKEEVVTVVLRVHMHCEACAMEIQKRIMRMKGVESVEPDFKASQVSVKGVFTPEKLVE 185

Query: 201 CIKKRMGKKVEIFNK----KSEDKSSNNKDSNVQHK 226
            I KR+GK   +  +    K  +K    KD + + K
Sbjct: 186 FIYKRIGKHAAVVKQDPPPKPPEKEKETKDKDEKKK 219

[Close]

BLAST of CSPI06G07680.1 vs. TAIR10
Match: AT5G63530.1 (AT5G63530.1 farnesylated protein 3)

HSP 1 Score: 117.5 bits (293), Expect = 1.2e-26
Identity = 80/201 (39.80%), Postives = 114/201 (56.72%), Query Frame = 1


Query: 25  KGQNQNGKRRQEEEKGKGKKEQKKIT------------EVVVKVYMHCEGCKHKLSKCLK 84
           KG++ + K+ QE E  K  KE                 EVV+KVYMHCEGC  K+ +CLK
Sbjct: 37  KGEDSD-KKPQEGESNKDSKEDSAPAAPEAPAPPPPPQEVVLKVYMHCEGCARKVRRCLK 96

Query: 85  ASRHGVEEVAADMENKKVVIKGNDLNPEKIMEEIKKKYSKNVELISPKELPDTSKQNKPE 144
               GVE+V  D +  KVV+KG   +P K++  +++K  + V+L+SP   P    + K E
Sbjct: 97  GFE-GVEDVMTDCKTGKVVVKGEKADPLKVLARVQRKTHRQVQLLSPIPPPPPPPEKKAE 156

Query: 145 PKKEEVQMKTVE--------LKMNMHCEGCENDIKQTIGKMEGVMRVETDRESSKVIVKG 204
             K  V+ K VE        LK++MHCE C  +IK+ I +M+GV   E+D +SS+V VKG
Sbjct: 157 EDKPIVEEKKVEPPVVVTVVLKVHMHCEACATEIKKRIMRMKGVESAESDLKSSQVTVKG 216

Query: 205 MIDPPKLMECIKKRMGKKVEI 206
           + +P KL+E + KR GK   I
Sbjct: 217 VFEPQKLVEYVYKRTGKHAAI 235

[Close]

BLAST of CSPI06G07680.1 vs. TAIR10
Match: AT5G60800.2 (AT5G60800.2 Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 98.2 bits (243), Expect = 7.6e-21
Identity = 73/216 (33.80%), Postives = 123/216 (56.94%), Query Frame = 1


Query: 23  MGKGQNQNGKRRQEEEKGKGKKEQKKITEVVVKVYMHCEGCKHKLSKCLKASRHGVEEVA 82
           MG+ +N+ G  +++    K K E   IT VV+KV MHCEGC  ++ KC++ S  GVE V 
Sbjct: 1   MGEKKNE-GDNKKKGGDNKKKNETPSIT-VVLKVDMHCEGCASRIVKCVR-SFQGVETVK 60

Query: 83  ADMENKKVVIKGNDLNPEKIMEEIKKKYSKNVELISPKELPDTSKQNKP----------- 142
           ++    K+ + G  L+P K+ E++++K  K V+L+SP+   +  K+NK            
Sbjct: 61  SESATGKLTVTGA-LDPVKLREKLEEKTKKKVDLVSPQPKKEKEKENKNKNDEDKKKSEE 120

Query: 143 -------EPKKEEVQMKTVELKMNMHCEGCENDIKQTIGKMEGVMRVETDRESSKVIVKG 202
                  + K +E  + T  LK+N HC+GC   I++T+ K +GV  +  D+E + + VKG
Sbjct: 121 KKKPDNNDKKPKETPVTTAVLKLNFHCQGCIGKIQKTVTKTKGVNGLTMDKEKNLLTVKG 180

Query: 203 MIDPPKLMECIKKRMGKKVEIF-NKKSEDKSSNNKD 220
            +D  KL+E + +++ + VEI   KK +DK + N++
Sbjct: 181 TMDVKKLVEILSEKLKRAVEIVPPKKEKDKENGNEN 212

[Close]

BLAST of CSPI06G07680.1 vs. TAIR10
Match: AT2G28090.1 (AT2G28090.1 Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 95.5 bits (236), Expect = 4.9e-20
Identity = 66/194 (34.02%), Postives = 111/194 (57.22%), Query Frame = 1


Query: 36  EEEKGKGKKEQKKITEVVVKVYMHCEGCKHKLSKCLKASRH--GVEEVAADMENKKVVIK 95
           EEEK K +        VV+K+  HC+GC   +++ ++ SR   GVE V AD ++ K+ + 
Sbjct: 15  EEEKKKKQNNTTSPVHVVLKIDFHCDGC---IARIVRLSRRLEGVETVRADPDSNKLTLI 74

Query: 96  GNDLNPEKIMEEIKKKYSKNVELISPKELPDTSKQNKPEPKKEE---VQMKTVELKMNMH 155
           G  ++P KI E+++KK  K VELISPK   DT + N+ +   +    V + TV LK+N  
Sbjct: 75  GFIMDPVKIAEKLQKKSKKKVELISPKPKKDTKENNEKKANDKTQTVVAVTTVVLKVNCS 134

Query: 156 CEGCENDIKQTIGKMEGVMRVETDRESSKVIVKGMIDPPKLMECIKKRMGKKVEIF-NKK 215
           C+GC   I++ +   +GV +V+ D+E   V V G +D   + + +K+++ K V++   KK
Sbjct: 135 CDGCIKRIQKAVSTTKGVYQVKMDKEKETVTVMGTMDIKSVTDNLKRKLKKTVQVVPEKK 194

Query: 216 SEDKSSNNKDSNVQ 224
            + K  +N + N +
Sbjct: 195 KKKKDKDNAEVNTK 205

[Close]

BLAST of CSPI06G07680.1 vs. NCBI nr
Match: gi|700191242|gb|KGN46446.1| (hypothetical protein Csa_6G094150 [Cucumis sativus])

HSP 1 Score: 486.9 bits (1252), Expect = 2.2e-134
Identity = 251/253 (99.21%), Postives = 252/253 (99.60%), Query Frame = 1


Query: 1   MDAITLSSHFFFQQWDFKSYKEMGKGQNQNGKRRQEEEKGKGKK-EQKKITEVVVKVYMH 60
           MDAITLSSHFFFQQWDFKSYKEMGKGQNQNGKRRQEEEKGKGKK EQKKITEVVVKVYMH
Sbjct: 1   MDAITLSSHFFFQQWDFKSYKEMGKGQNQNGKRRQEEEKGKGKKEEQKKITEVVVKVYMH 60

Query: 61  CEGCKHKLSKCLKASRHGVEEVAADMENKKVVIKGNDLNPEKIMEEIKKKYSKNVELISP 120
           CEGCKHKLSKCLKASRHGVEEVAADMENKKVVIKGNDLNPE+IMEEIKKKYSKNVELISP
Sbjct: 61  CEGCKHKLSKCLKASRHGVEEVAADMENKKVVIKGNDLNPERIMEEIKKKYSKNVELISP 120

Query: 121 KELPDTSKQNKPEPKKEEVQMKTVELKMNMHCEGCENDIKQTIGKMEGVMRVETDRESSK 180
           KELPDTSKQNKPEPKKEEVQMKTVELKMNMHCEGCENDIKQTIGKMEGVMRVETDRESSK
Sbjct: 121 KELPDTSKQNKPEPKKEEVQMKTVELKMNMHCEGCENDIKQTIGKMEGVMRVETDRESSK 180

Query: 181 VIVKGMIDPPKLMECIKKRMGKKVEIFNKKSEDKSSNNKDSNVQHKLDHFTFKYPPQYSL 240
           VIVKGMIDPPKLMECIKKRMGKKVEIFNKKSEDKSSNNKDSNVQHKLDHFTFKYPPQYSL
Sbjct: 181 VIVKGMIDPPKLMECIKKRMGKKVEIFNKKSEDKSSNNKDSNVQHKLDHFTFKYPPQYSL 240

Query: 241 LHIYPNQTFSDDN 253
           LHIYPNQTFSDDN
Sbjct: 241 LHIYPNQTFSDDN 253

[Close]

BLAST of CSPI06G07680.1 vs. NCBI nr
Match: gi|659120430|ref|XP_008460190.1| (PREDICTED: uncharacterized protein PF11_0207 [Cucumis melo])

HSP 1 Score: 384.8 bits (987), Expect = 1.2e-103
Identity = 202/237 (85.23%), Postives = 219/237 (92.41%), Query Frame = 1


Query: 23  MGKGQNQNGKRRQEEEKGKGKKEQKK---ITEVVVKVYMHCEGCKHKLSKCLKASRHGVE 82
           MGKGQNQNG ++QEEEKGKGKKE+++   ITEVV+KVYMHCE C+HKLSKCLKAS HGVE
Sbjct: 1   MGKGQNQNGNKKQEEEKGKGKKEEQQKKIITEVVIKVYMHCEACQHKLSKCLKASSHGVE 60

Query: 83  EVAADMENKKVVIKGNDLNPEKIMEEIKKKYSKNVELISPKELPDTSKQNKPEPKKEEVQ 142
           EVAADMENKKVVIKG DLNP+KIMEEIKKKYSKNVELISPK+LPDT KQNKP+PKKEEVQ
Sbjct: 61  EVAADMENKKVVIKGKDLNPKKIMEEIKKKYSKNVELISPKDLPDTPKQNKPQPKKEEVQ 120

Query: 143 MKTVELKMNMHCEGCENDIKQTIGKMEGVMRVETDRESSKVIVKGMIDPPKLMECIKKRM 202
           MKTVELKMNMHCEGCENDIK+TIGKMEGVMRVET+RESSKVIVKGMIDPPKLMECIKK+M
Sbjct: 121 MKTVELKMNMHCEGCENDIKETIGKMEGVMRVETNRESSKVIVKGMIDPPKLMECIKKKM 180

Query: 203 GKKVEIFNKKS----EDKSSNNKDSNVQHKLDHFTFKYPPQYSLLHIYPNQTFSDDN 253
           GK+VEI N K     EDKS+NNKDSN + KLD+F FKYPPQYSLLHIYPNQTFSDDN
Sbjct: 181 GKEVEILNNKKDGKPEDKSNNNKDSN-EEKLDNFAFKYPPQYSLLHIYPNQTFSDDN 236

[Close]

BLAST of CSPI06G07680.1 vs. NCBI nr
Match: gi|778722058|ref|XP_011658396.1| (PREDICTED: uncharacterized protein LOC105435985 [Cucumis sativus])

HSP 1 Score: 340.5 bits (872), Expect = 2.5e-90
Identity = 174/175 (99.43%), Postives = 175/175 (100.00%), Query Frame = 1


Query: 78  VEEVAADMENKKVVIKGNDLNPEKIMEEIKKKYSKNVELISPKELPDTSKQNKPEPKKEE 137
           VEEVAADMENKKVVIKGNDLNPE+IMEEIKKKYSKNVELISPKELPDTSKQNKPEPKKEE
Sbjct: 74  VEEVAADMENKKVVIKGNDLNPERIMEEIKKKYSKNVELISPKELPDTSKQNKPEPKKEE 133

Query: 138 VQMKTVELKMNMHCEGCENDIKQTIGKMEGVMRVETDRESSKVIVKGMIDPPKLMECIKK 197
           VQMKTVELKMNMHCEGCENDIKQTIGKMEGVMRVETDRESSKVIVKGMIDPPKLMECIKK
Sbjct: 134 VQMKTVELKMNMHCEGCENDIKQTIGKMEGVMRVETDRESSKVIVKGMIDPPKLMECIKK 193

Query: 198 RMGKKVEIFNKKSEDKSSNNKDSNVQHKLDHFTFKYPPQYSLLHIYPNQTFSDDN 253
           RMGKKVEIFNKKSEDKSSNNKDSNVQHKLDHFTFKYPPQYSLLHIYPNQTFSDDN
Sbjct: 194 RMGKKVEIFNKKSEDKSSNNKDSNVQHKLDHFTFKYPPQYSLLHIYPNQTFSDDN 248

[Close]

BLAST of CSPI06G07680.1 vs. NCBI nr
Match: gi|596299324|ref|XP_007227635.1| (hypothetical protein PRUPE_ppa022179mg, partial [Prunus persica])

HSP 1 Score: 207.6 bits (527), Expect = 2.5e-50
Identity = 113/230 (49.13%), Postives = 161/230 (70.00%), Query Frame = 1


Query: 25  KGQNQNGKRRQEEEKGKGKKEQKKITEVVVKVYMHCEGCKHKLSKCLKASRHGVEEVAAD 84
           +  NQN ++ Q++EK     +     E+V++V MHCEGCK K++KCLKA   G+E+V  D
Sbjct: 6   ENNNQNAEKNQQDEKNSQSSK-----EIVLRVLMHCEGCKSKVAKCLKAVE-GIEDVTVD 65

Query: 85  MENKKVVIKGNDLNPEKIMEEIKKKYSKNVELISPKELPDTSKQNKPEPKKE-EVQMKTV 144
             N++VV+KG   NP K++E ++KKYS N ELISPK  P+  ++ +P+ K+E + Q+K V
Sbjct: 66  YANQRVVVKGKKANPLKVLERLQKKYSSNAELISPKPKPENKEKKEPQKKEESQPQVKVV 125

Query: 145 ELKMNMHCEGCENDIKQTIGKMEGVMRVETDRESSKVIVKGMIDPPKLMECIKKRMGKKV 204
            LKM MHCEGC NDIK+ + KM+GV+ VE + ESS+V V+G++DPPKL+E IKK++GK  
Sbjct: 126 VLKMLMHCEGCANDIKKYLEKMKGVLSVEANMESSRVTVRGVVDPPKLIEYIKKQLGKHA 185

Query: 205 EIFNKKSEDKSSNNKDSNVQHKLDHFT-FKYPPQYSLLHIYPNQTFSDDN 253
           EI  ++   K  NN ++N +   +    F+YPPQYS  HIYPNQTFSD+N
Sbjct: 186 EIVKQEQGQKQGNNNNNNNKKDPERENIFQYPPQYSSQHIYPNQTFSDEN 229

[Close]

BLAST of CSPI06G07680.1 vs. NCBI nr
Match: gi|590691910|ref|XP_007043915.1| (Heavy metal transport/detoxification superfamily protein, putative [Theobroma cacao])

HSP 1 Score: 193.7 bits (491), Expect = 3.8e-46
Identity = 114/239 (47.70%), Postives = 150/239 (62.76%), Query Frame = 1


Query: 23  MGKGQNQNG---------KRRQEEEKGKGKKEQKKITEVVVKVYMHCEGCKHKLSKCLKA 82
           MG GQ   G         ++  E  + KG K+  K  E+V+KVYMHCEGC  K+  CLK 
Sbjct: 1   MGHGQQHQGGGQIVEVKLEKSCENNQEKGAKDSNKGKEIVLKVYMHCEGCAAKVFNCLKG 60

Query: 83  SRHGVEEVAADMENKKVVIKGNDLNPEKIMEEIKKKYSKNVELISPKELPDTSKQNKPEP 142
            + GVE+V  DME  +V++KG + +P KI+E +KKKYS+N ELISPK  P  +   K   
Sbjct: 61  FQ-GVEQVKTDMEGDRVIVKGQNADPLKILERVKKKYSRNAELISPKPKPKAT-DGKQSQ 120

Query: 143 KKEEVQMKTVELKMNMHCEGCENDIKQTIGKMEGVMRVETDRESSKVIVKGMIDPPKLME 202
            K+E  +K V LKM MHCEGC NDIK++IG+M+G++ VE D + S V V+G  DPPKL+E
Sbjct: 121 NKQEPPIKFVVLKMYMHCEGCANDIKRSIGRMKGILNVEPDMKKSTVTVRGNFDPPKLVE 180

Query: 203 CIKKRMGKKVEIFNKKSEDKSSNNKDSNVQHKLDHFTFKYPPQYSLLHIYPNQTFSDDN 253
            I K+ GK  EI  +   DK++    +  Q K +   F YPPQYSL HIYP Q FSD+N
Sbjct: 181 AIAKQFGKYAEIVAEGPTDKANG---TGKQGKEEEIMFHYPPQYSLQHIYPTQIFSDEN 234

The following BLAST results are available for this feature:

BLAST of CSPI06G07680.1 vs. Swiss-Prot


Match Name	E-value	Identity	Description
HIP3_ARATH	1.4e-19	33.80	Heavy metal-associated isoprenylated plant protein 3 OS=Arabidopsis thaliana GN=...	[more]
HIP26_ARATH	7.0e-08	38.37	Heavy metal-associated isoprenylated plant protein 26 OS=Arabidopsis thaliana GN...	[more]
HIP27_ARATH	7.0e-08	37.50	Heavy metal-associated isoprenylated plant protein 27 OS=Arabidopsis thaliana GN...	[more]
HIP20_ARATH	7.0e-08	39.73	Heavy metal-associated isoprenylated plant protein 20 OS=Arabidopsis thaliana GN...	[more]
HIP21_ARATH	2.6e-07	35.62	Heavy metal-associated isoprenylated plant protein 21 OS=Arabidopsis thaliana GN...	[more]

BLAST of CSPI06G07680.1 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KFC0_CUCSA	1.5e-134	99.21	Uncharacterized protein OS=Cucumis sativus GN=Csa_6G094150 PE=4 SV=1	[more]
M5XSC8_PRUPE	1.8e-50	49.13	Uncharacterized protein (Fragment) OS=Prunus persica GN=PRUPE_ppa022179mg PE=4 S...	[more]
A0A061E5Z1_THECC	2.6e-46	47.70	Heavy metal transport/detoxification superfamily protein, putative OS=Theobroma ...	[more]
A0A072UU25_MEDTR	2.2e-45	46.44	Heavy metal transport/detoxification superfamily protein OS=Medicago truncatula ...	[more]
R0HIQ9_9BRAS	2.5e-44	45.45	Uncharacterized protein (Fragment) OS=Capsella rubella GN=CARUB_v10015807mg PE=4...	[more]

BLAST of CSPI06G07680.1 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G02960.1	1.7e-44	43.88	Heavy metal transport/detoxification superfamily protein	[more]
AT5G50740.3	5.8e-29	35.65	Heavy metal transport/detoxification superfamily protein	[more]
AT5G63530.1	1.2e-26	39.80	farnesylated protein 3	[more]
AT5G60800.2	7.6e-21	33.80	Heavy metal transport/detoxification superfamily protein	[more]
AT2G28090.1	4.9e-20	34.02	Heavy metal transport/detoxification superfamily protein	[more]

BLAST of CSPI06G07680.1 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700191242\|gb\|KGN46446.1\|	2.2e-134	99.21	hypothetical protein Csa_6G094150 [Cucumis sativus]	[more]
gi\|659120430\|ref\|XP_008460190.1\|	1.2e-103	85.23	PREDICTED: uncharacterized protein PF11_0207 [Cucumis melo]	[more]
gi\|778722058\|ref\|XP_011658396.1\|	2.5e-90	99.43	PREDICTED: uncharacterized protein LOC105435985 [Cucumis sativus]	[more]
gi\|596299324\|ref\|XP_007227635.1\|	2.5e-50	49.13	hypothetical protein PRUPE_ppa022179mg, partial [Prunus persica]	[more]
gi\|590691910\|ref\|XP_007043915.1\|	3.8e-46	47.70	Heavy metal transport/detoxification superfamily protein, putative [Theobroma ca...	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **INTERPRO**
Term	Definition
IPR006121	HMA_dom

Vocabulary: **Biological Process**
Term	Definition
GO:0030001	metal ion transport

Vocabulary: **Molecular Function**
Term	Definition
GO:0046872	metal ion binding

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030001	metal ion transport
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0046872	metal ion binding

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CSPI06G07680	CSPI06G07680	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CSPI06G07680.1	CSPI06G07680.1-protein	polypeptide

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI06G07680.1.cds5	CSPI06G07680.1.cds5	CDS
CSPI06G07680.1.cds4	CSPI06G07680.1.cds4	CDS
CSPI06G07680.1.cds3	CSPI06G07680.1.cds3	CDS
CSPI06G07680.1.cds2	CSPI06G07680.1.cds2	CDS
CSPI06G07680.1.cds1	CSPI06G07680.1.cds1	CDS

InterPro

Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006121	Heavy metal-associated domain, HMA	PFAM	PF00403	HMA	coord: 146..201 score: 2.0E-10coord: 54..109 score: 6.
IPR006121	Heavy metal-associated domain, HMA	PROFILE	PS50846	HMA_2	coord: 58..108 score: 9.756coord: 148..197 score: 10
IPR006121	Heavy metal-associated domain, HMA	unknown	SSF55008	HMA, heavy metal-associated domain	coord: 139..204 score: 1.24E-13coord: 52..108 score: 8.64
None	No IPR available	GENE3D	G3DSA:3.30.70.100		coord: 49..112 score: 1.1E-11coord: 138..205 score: 2.1
None	No IPR available	PANTHER	PTHR22814	COPPER TRANSPORT PROTEIN ATOX1-RELATED	coord: 221..252 score: 1.1E-50coord: 40..204 score: 1.1
None	No IPR available	PANTHER	PTHR22814:SF109	F13E7.9 PROTEIN	coord: 221..252 score: 1.1E-50coord: 40..204 score: 1.1