CSPI04G13500.1 (mRNA) Wild cucumber (PI 183967)

BLAST of CSPI04G13500.1 vs. Swiss-Prot
Match: LOX21_SOLTU (Linoleate 13S-lipoxygenase 2-1, chloroplastic OS=Solanum tuberosum GN=LOX2.1 PE=1 SV=1)

HSP 1 Score: 668.7 bits (1724), Expect = 7.9e-191
Identity = 385/782 (49.23%), Postives = 490/782 (62.66%), Query Frame = 1

Query: 95  YKGDIKVAEGFGKIGAVIVELQDGVNERFVDTIYVEAEKPPISVTFSCKSWVQPKGLIDH 154
           Y+ D  + + FG++GA+++E  +   E +V  I ++       V  +C SWV  K     
Sbjct: 137 YEADFVIPQDFGEVGAILIE-NEHHKEMYVKNIVIDGFVHG-KVEITCNSWVHSKFDNPD 196

Query: 155 QRIFFSSNKSYLSGKTPGGLVKLREEDLANLRGEKADGSVDRNERKAFERIYDYDLYNDL 214
           +RIFF+ NKSYL  +TP G+ +LREE+L  LRG+         ERK FERIYDYD+YNDL
Sbjct: 197 KRIFFT-NKSYLPSQTPSGVSRLREEELVTLRGDGI------GERKVFERIYDYDVYNDL 256

Query: 215 GDPDQSMN-LKRPVLG-------------------DPASEKRIEEWFYVPRDEEFSEIKQ 274
           G+ D + +  KRPVLG                   DP SE R   + YVPRDE FSE+K 
Sbjct: 257 GEADSNNDDAKRPVLGGKELPYPRRCKTGRPRSKKDPLSETR-STFVYVPRDEAFSEVK- 316

Query: 275 SSSQPGNEKLLRKSPFSDLPELEIKT----------PSAASKFNLQFNIASI------LS 334
           S +  GN   +     + +P LE             P+  S FN+  ++  +      L 
Sbjct: 317 SVAFSGNT--VYSVLHAVVPALESVVTDPNLGFPHFPAIDSLFNVGVDLPGLGDKKSGLF 376

Query: 335 SHRPPALKS-SDTPSSTLVELPPPESYKRDKYNWLSDIEFARLTLAGLNPYSIQLV---- 394
           +  P  +K+ SDT    L+    P+  +RDK++W  D+EFAR TLAGLNPYSI+LV    
Sbjct: 377 NVVPRLIKAISDTRKDVLL-FESPQLVQRDKFSWFRDVEFARQTLAGLNPYSIRLVTEWP 436

Query: 395 ------------------KALADKR---------------LFVVDYHDTLMPYVSKVRKI 454
                             K L +K                LF++DYHD L+PYV+KV ++
Sbjct: 437 LRSKLDPKVYGPPESEITKELIEKEIGNYMTVEQAVQQKKLFILDYHDLLLPYVNKVNEL 496

Query: 455 KGTTLYGSRTLFFLNSDGTLMPLGIELTRPPMDGHLEQWKEVFTPGT-NSTDVWLWRLAK 514
           KG+ LYGSRT+FFL   GTL PL IELTRPP+D    QWKEV++P   N+T  WLW+LAK
Sbjct: 497 KGSMLYGSRTIFFLTPQGTLKPLAIELTRPPVDDK-PQWKEVYSPNDWNATGAWLWKLAK 556

Query: 515 AHVLSHDSCIHQLVIHWLRAHCCMEPYAIATNRQLSTMHPIYRLLHPHFRYNMRINANAR 574
           AHVLSHDS  HQLV HWLR HCC EPY IA+NRQLS MHPIYRLLHPHFRY M INA AR
Sbjct: 557 AHVLSHDSGYHQLVSHWLRTHCCTEPYIIASNRQLSAMHPIYRLLHPHFRYTMEINALAR 616

Query: 575 KNLINAGGIIENAFSGASYSVELSSSAYKA-WRFDEQALPEDLIHRGMA-ERKRDSEGRD 634
           + LINA G+IE++F    Y++ELSS AY A WRFD++ALP++LI RG+A E   +  G  
Sbjct: 617 EALINANGVIESSFFPGKYAIELSSIAYGAEWRFDQEALPQNLISRGLAVEDPNEPHG-- 676

Query: 635 VLELTIKDYPFANDGLLLWNALLEWVTEYVNHYYGDEENAVTNDKELQAWWGEIQAKGHP 694
            L+L I+DYPFANDGL+LW+ L +WVT YVNHYY  + N + +DKELQAWW EI+  GH 
Sbjct: 677 -LKLAIEDYPFANDGLVLWDILKQWVTNYVNHYY-PQTNLIESDKELQAWWSEIKNVGHG 736

Query: 695 DKRE--GWPALRTRDDLIKIASTIAWVGSGHHASVNFLQYAYGGYMPNRPTIARTNMLTE 754
           DKR+   WP L+T +DLI I +TI WV SGHHA+VNF QY+Y GY PNRPT+AR+ M TE
Sbjct: 737 DKRDEPWWPELKTPNDLIGIITTIVWVTSGHHAAVNFGQYSYAGYFPNRPTVARSKMPTE 796

Query: 755 NHSDEILKDFRNYPENKLNELFPSVVQAAIVKQTMFLLSIHSPDEEYIGDAIEPAWALDH 798
           + + E  + F N PE  L   FPS +QA  V   + +LS HSPDEEYIG+ IEP WA D 
Sbjct: 797 DPTAEEWEWFMNKPEEALLRCFPSQIQATKVMAILDVLSNHSPDEEYIGEKIEPYWAEDP 856

BLAST of CSPI04G13500.1 vs. Swiss-Prot
Match: LOX2_ARATH (Lipoxygenase 2, chloroplastic OS=Arabidopsis thaliana GN=LOX2 PE=1 SV=1)

HSP 1 Score: 655.2 bits (1689), Expect = 9.1e-187
Identity = 371/801 (46.32%), Postives = 475/801 (59.30%), Query Frame = 1

Query: 77  IKCEAQLQEVDESVEYKKYKGDIKVAEGFGKIGAVIVELQDGVNERFVDTIYVEAEKPPI 136
           I  E   Q V      +KY+ + ++ E FG +GA+ ++ Q     R +    VE + P  
Sbjct: 119 ITVEDYAQRVWAEAPDEKYECEFEMPEDFGPVGAIKIQNQ---YHRQLFLKGVELKLPGG 178

Query: 137 SVTFSCKSWVQPKGLIDHQRIFFSSNKSYLSGKTPGGLVKLREEDLANLRGEKADGSVDR 196
           S+TF+C+SWV PK +   +RIFFS +KSYL  +TP  L K R+E+L  L+G+  +   + 
Sbjct: 179 SITFTCESWVAPKSVDPTKRIFFS-DKSYLPSQTPEPLKKYRKEELETLQGKNRE---EV 238

Query: 197 NERKAFERIYDYDLYNDLGDPDQSMNLKRPVLG-------------------DPASEKRI 256
            E   FERIYDYD+YND+GDPD    L RPV+G                   DP+SE+R 
Sbjct: 239 GEFTKFERIYDYDVYNDVGDPDNDPELARPVIGGLTHPYPRRCKTGRKPCETDPSSEQRY 298

Query: 257 EEWFYVPRD---------------------EEFSEIKQSSSQPGNEKLLRKSPFSDLPEL 316
              FYVPRD                       F +I+     P  E         +L E 
Sbjct: 299 GGEFYVPRDEEFSTAKGTSFTGKAVLAALPSIFPQIESVLLSP-QEPFPHFKAIQNLFEE 358

Query: 317 EIKTPSAASKFNLQFNIASILSSHRPPALKSSDTPSSTLVELPPPESYKRDKYNWLSDIE 376
            I+ P  A    L            P  +K+       +++   P    RD+++WL D E
Sbjct: 359 GIQLPKDAGLLPLL-----------PRIIKALGEAQDDILQFDAPVLINRDRFSWLRDDE 418

Query: 377 FARLTLAGLNPYSIQLVK-----------ALADKRLFVVD-------------------- 436
           FAR TLAGLNPYSIQLV+              D    +                      
Sbjct: 419 FARQTLAGLNPYSIQLVEEWPLISKLDPAVYGDPTSLITWEIVEREVKGNMTVDEALKNK 478

Query: 437 ------YHDTLMPYVSKVRKIKGTTLYGSRTLFFLNSDGTLMPLGIELTRPPMDGHLEQW 496
                 YHD L+PYV+KVR++  TTLY SRTLFFL+ D TL P+ IELT PP + +  QW
Sbjct: 479 RLFVLDYHDLLLPYVNKVRELNNTTLYASRTLFFLSDDSTLRPVAIELTCPP-NINKPQW 538

Query: 497 KEVFTPGTNSTDVWLWRLAKAHVLSHDSCIHQLVIHWLRAHCCMEPYAIATNRQLSTMHP 556
           K+VFTPG ++T  WLW LAK H +SHD+  HQL+ HWLR H C EPY IA NRQLS MHP
Sbjct: 539 KQVFTPGYDATSCWLWNLAKTHAISHDAGYHQLISHWLRTHACTEPYIIAANRQLSAMHP 598

Query: 557 IYRLLHPHFRYNMRINANARKNLINAGGIIENAFSGASYSVELSSSAY-KAWRFDEQALP 616
           IYRLLHPHFRY M INA AR++L+N GGIIE  F    Y++ELSS+ Y K WRFD++ LP
Sbjct: 599 IYRLLHPHFRYTMEINARARQSLVNGGGIIETCFWPGKYALELSSAVYGKLWRFDQEGLP 658

Query: 617 EDLIHRGMAERKRDSEGRDVLELTIKDYPFANDGLLLWNALLEWVTEYVNHYYGDEENAV 676
            DLI RG+AE  + +E    + LTI DYPFANDGL+LW+A+ EWVT+YV HYY DEE  +
Sbjct: 659 ADLIKRGLAEEDKTAE--HGVRLTIPDYPFANDGLILWDAIKEWVTDYVKHYYPDEE-LI 718

Query: 677 TNDKELQAWWGEIQAKGHPDKRE--GWPALRTRDDLIKIASTIAWVGSGHHASVNFLQYA 736
           T+D+ELQ WW E++  GH DK++   WP L+T+DDLI + +TIAWV SGHHA+VNF QY 
Sbjct: 719 TSDEELQGWWSEVRNIGHGDKKDEPWWPVLKTQDDLIGVVTTIAWVTSGHHAAVNFGQYG 778

Query: 737 YGGYMPNRPTIARTNMLTENHSDEILKDFRNYPENKLNELFPSVVQAAIVKQTMFLLSIH 796
           YGGY PNRPT  R  M TE+ +DE LK+F   PE  L + +PS  QA +V  T+ LLS H
Sbjct: 779 YGGYFPNRPTTTRIRMPTEDPTDEALKEFYESPEKVLLKTYPSQKQATLVMVTLDLLSTH 838

Query: 797 SPDEEYIGDAIEPAWALDHSISKAFEKFKAKLTDLEKKIDELNENKDLKNRYGAGIIPYE 798
           SPDEEYIG+  E +WA +  I+ AFE+FK KL  LE  IDE N N  LKNR GAG++ YE
Sbjct: 839 SPDEEYIGEQQEASWANEPVINAAFERFKGKLQYLEGVIDERNVNITLKNRAGAGVVKYE 896

BLAST of CSPI04G13500.1 vs. Swiss-Prot
Match: LOXC1_ORYSJ (Lipoxygenase 7, chloroplastic OS=Oryza sativa subsp. japonica GN=CM-LOX1 PE=2 SV=2)

HSP 1 Score: 614.4 bits (1583), Expect = 1.8e-174
Identity = 373/863 (43.22%), Postives = 494/863 (57.24%), Query Frame = 1

Query: 33  RMSYMGTVVVTVIPKKNEQFPWVEII----YLKFASVELDSNEQPKEFIKCEAQLQEVDE 92
           R+  + T+ VTV    N      ++I     L+  S ELD+ +  KE     +    VD+
Sbjct: 85  RVKAVATIKVTVGELINRSIDIRDLIGRSLSLELVSSELDA-KTGKEKATVRSYAHNVDD 144

Query: 93  SVE-YKKYKGDIKVAEGFGKIGAVIV--ELQDGVNERFVDTIYVEAEKPPISVT---FSC 152
                  Y+ D  V  GFG IGA+IV  EL+    E F++ I + A     + T     C
Sbjct: 145 DDHSVVTYEADFDVPSGFGPIGAIIVTNELRQ---EMFLEDINLTASDGAGNSTVLPIRC 204

Query: 153 KSWVQPKGLIDH----QRIFFSSNKSYLSGKTPGGLVKLREEDLANLRGEKADGSVDRNE 212
            SWVQPK + D     +RIFF+ NK+YL G+TP GL   R+ DL   RG   DG+    E
Sbjct: 205 NSWVQPKSVGDEGTPSKRIFFA-NKTYLPGQTPAGLRSYRKNDLQQKRG---DGT---GE 264

Query: 213 RKAFERIYDYDLYNDLGDPDQSMNLKRPVLG--------------------DPASEKRIE 272
           R+A +R+YDYD+YNDLG+PD + +L RPVLG                    DP SE R  
Sbjct: 265 READDRVYDYDVYNDLGNPDSNGDLARPVLGGNKQFPYPRRCRTGRPPSKKDPKSETRKG 324

Query: 273 EWFYVPRDEEFSEIKQS------------SSQPGNEKLLRKSPFSDLP-----------E 332
              YVPRDEEFS  K+             ++ P  + LL      +LP           E
Sbjct: 325 N-VYVPRDEEFSPEKEDYFLRKTVGSVLQAAVPAAQSLLLDKLKWNLPFPSFFVIDKLFE 384

Query: 333 LEIKTPSAASKFNLQFNIASILSSHRPPALKSSDTPSSTLVELPPPESYKRDKYNWLSDI 392
             ++ P    K N   ++   L  H        DTP+  ++    P + ++DK+ WL D 
Sbjct: 385 DGVELPGV-DKLNFLESVVPRLLEHL------RDTPAEKILRFETPANIQKDKFAWLRDE 444

Query: 393 EFARLTLAGLNPYSIQLVK-------------------------------------ALAD 452
           EFAR TLAG+NPY+I+LV+                                     A++ 
Sbjct: 445 EFARETLAGINPYAIELVREFPLKSKLDPAVYGPAESAITADLLEEQMRRVMTVEEAISQ 504

Query: 453 KRLFVVDYHDTLMPYVSKVRKIKGTTLYGSRTLFFLNSDGTLMPLGIELTRPPMDGHLEQ 512
           KRLF++D+HD  +PYV K+R +  TT+YGSRT+FFL  DGTL  L IELTRP       Q
Sbjct: 505 KRLFMLDFHDLFLPYVHKIRSLDHTTMYGSRTVFFLTDDGTLQLLAIELTRPASPSQ-PQ 564

Query: 513 WKEVFTPGTNSTDVWLWRLAKAHVLSHDSCIHQLVIHWLRAHCCMEPYAIATNRQLSTMH 572
           W++VFTP T++T  WLWR+AKAHV +HD+  H+L+ HWLR HC +EPY IA NRQLS MH
Sbjct: 565 WRQVFTPSTDATMSWLWRMAKAHVRAHDAGHHELITHWLRTHCAVEPYIIAANRQLSEMH 624

Query: 573 PIYRLLHPHFRYNMRINANARKNLINAGGIIENAFSGASYSVELSSSAY-KAWRFDEQAL 632
           PIY+LL PHFRY MRINA AR  LI+AGGIIE +FS   YS+ELSS AY K WRFD +AL
Sbjct: 625 PIYQLLRPHFRYTMRINARARSALISAGGIIERSFSPQKYSMELSSVAYDKLWRFDTEAL 684

Query: 633 PEDLIHRGMAERKRDSEGRDVLELTIKDYPFANDGLLLWNALLEWVTEYVNHYYGDEENA 692
           P DL+ RGMAE   D      L+L I+DYPFANDGLL+W+A+  WV  YV  +Y D + +
Sbjct: 685 PADLVRRGMAE--EDPTAEHGLKLAIEDYPFANDGLLIWDAIKTWVQAYVARFYPDAD-S 744

Query: 693 VTNDKELQAWWGEIQAKGHPDKREG--WPALRTRDDLIKIASTIAWVGSGHHASVNFLQY 752
           V  D+ELQA+W E++ KGH DK++   WP L + + L    +TI WV + HHA+VNF QY
Sbjct: 745 VAGDEELQAFWTEVRTKGHGDKKDAPWWPKLDSPESLAHTLTTIVWVAAAHHAAVNFGQY 804

Query: 753 AYGGYMPNRPTIARTNMLTENHSD-EILKDFRNYPENKLNELFPSVVQAAIVKQTMFLLS 798
            +GGY PNRP+IART M  E   D   ++ F + P+  L E FPS VQA +V   + +LS
Sbjct: 805 DFGGYFPNRPSIARTVMPVEEPVDGAAMERFLDNPDQALRECFPSQVQATVVMAVLDVLS 864

BLAST of CSPI04G13500.1 vs. Swiss-Prot
Match: LOXC2_ORYSJ (Probable lipoxygenase 8, chloroplastic OS=Oryza sativa subsp. japonica GN=CM-LOX2 PE=2 SV=1)

HSP 1 Score: 614.4 bits (1583), Expect = 1.8e-174
Identity = 360/831 (43.32%), Postives = 478/831 (57.52%), Query Frame = 1

Query: 58  IYLKFASVELDSNEQPKEFIKCEAQLQEVDESVEYKKYKGDIKVAEGFGKIGAVIVELQD 117
           ++L+  S EL++    K+        +  D+      Y+ D  V  GFG IGAV+V  + 
Sbjct: 132 LFLELVSSELEAKTGKKKATVHSYAHKVDDDDHGVVTYEADFDVPTGFGPIGAVVVTNEL 191

Query: 118 GVNERFVDTIYVEAEKPPISVT---FSCKSWVQPKGLIDH----QRIFFSSNKSYLSGKT 177
           G  E F++ + + A     + T     C SWVQPK  ID     +RIFF+  K+YL G+T
Sbjct: 192 G-QEMFLEDLNLTAGDGAGNSTVLPIRCNSWVQPKSSIDEGTPGKRIFFA--KAYLPGQT 251

Query: 178 PGGLVKLREEDLANLRGEKADGSVDRNERKAFERIYDYDLYNDLGDPDQSMNLKRPVLG- 237
           P GL   REEDL   RG  A       +R+A +R+YDYD+YNDLG+PD + +L RPVLG 
Sbjct: 252 PAGLRSYREEDLKQKRGNGA------GQREADDRVYDYDVYNDLGNPDSNGDLARPVLGG 311

Query: 238 -------------------DPASEKRIEEWFYVPRDEEFSEIKQS------------SSQ 297
                              DP SE R     YVPRDEEFSE+K +            ++ 
Sbjct: 312 SKQFPYPRRCRTGRPPSKKDPKSETRKGN-VYVPRDEEFSEVKNAQFLLKTLQSVLHAAV 371

Query: 298 PGNEKLLRKSPFSDLP-----------ELEIKTPSAASKFNLQFNIASILSSHRPPALKS 357
           P  +  L  +   +LP           E  ++ P       L   +  +L   R      
Sbjct: 372 PAAQSALIDNLSLNLPFPSFFVIDKLFEDGVELPGVEKLGFLHSIVPRLLELLR------ 431

Query: 358 SDTPSSTLVELPPPESYKRDKYNWLSDIEFARLTLAGLNPYSIQLVK------------- 417
            D+P   ++    P + ++DK+ WL D EFAR TLAG+NPY+I+LV+             
Sbjct: 432 -DSPGDKILLFDTPANVQKDKFAWLRDEEFARETLAGINPYAIELVREFPLKSKLDPAVY 491

Query: 418 ------------------------ALADKRLFVVDYHDTLMPYVSKVRKIKGTTLYGSRT 477
                                   A++ KRLF++D+HD  +PYV K+R +K TT+YGSRT
Sbjct: 492 GPAESAITADLLEEQMRRVMTVEEAISQKRLFMLDFHDLFLPYVHKIRSLKHTTMYGSRT 551

Query: 478 LFFLNSDGTLMPLGIELTRPPMDGHLEQWKEVFTPGTNSTDVWLWRLAKAHVLSHDSCIH 537
           +FFL  DGTL  L IELTRP       QW++VFTP T++T  WLWR+AKAHV +HD+  H
Sbjct: 552 IFFLTDDGTLRLLAIELTRPASPSQ-PQWRQVFTPSTDTTKSWLWRMAKAHVRAHDAGHH 611

Query: 538 QLVIHWLRAHCCMEPYAIATNRQLSTMHPIYRLLHPHFRYNMRINANARKNLINAGGIIE 597
           +L+ HWLR HC +EPY IA NRQLS MHPIY+LLHPHFRY MRINA AR  LI+A GIIE
Sbjct: 612 ELITHWLRTHCAVEPYIIAANRQLSEMHPIYQLLHPHFRYTMRINALARSRLISAAGIIE 671

Query: 598 NAFSGASYSVELSSSAY-KAWRFDEQALPEDLIHRGMAERKRDSEGRDVLELTIKDYPFA 657
            +FS   YS+ELSS AY K WRFD +ALP DL+ RGMAE   D      L L I+DYPFA
Sbjct: 672 LSFSPQKYSMELSSVAYDKLWRFDMEALPADLVRRGMAE--EDPTAEHGLRLAIEDYPFA 731

Query: 658 NDGLLLWNALLEWVTEYVNHYYGDEENAVTNDKELQAWWGEIQAKGHPDKREG--WPALR 717
           NDGLL+W+A+  WV  YV  +Y D + +V  D+ELQA+W E++ KGH DK++   WP L 
Sbjct: 732 NDGLLIWDAIKTWVQAYVARFYPDAD-SVAGDEELQAFWTEVRTKGHGDKKDAPWWPKLD 791

Query: 718 TRDDLIKIASTIAWVGSGHHASVNFLQYAYGGYMPNRPTIARTNMLTENHSD-EILKDFR 777
           + + L    +TI WV + HHA+VNF QY +GGY PNRP+IART M  E   D   ++ F 
Sbjct: 792 SPESLAHTLTTIVWVAAAHHAAVNFGQYDFGGYFPNRPSIARTVMPVEEPVDGAAMERFL 851

Query: 778 NYPENKLNELFPSVVQAAIVKQTMFLLSIHSPDEEYIGDAIEPAWALDHSISKAFEKFKA 798
           + P+  L E FPS VQA +V   + +LS HS DEEY+G      W  D ++  A+  F A
Sbjct: 852 DNPDQALRECFPSQVQATVVMAVLDVLSTHSTDEEYLGGEQTRPWNSDAAVQAAYAGFTA 911

BLAST of CSPI04G13500.1 vs. Swiss-Prot
Match: LOX23_HORVU (Lipoxygenase 2.3, chloroplastic OS=Hordeum vulgare GN=LOX2.3 PE=1 SV=1)

HSP 1 Score: 520.0 bits (1338), Expect = 4.6e-146
Identity = 251/447 (56.15%), Postives = 325/447 (72.71%), Query Frame = 1

Query: 354 KALADKRLFVVDYHDTLMPYVSKVRKIKGTTLYGSRTLFFLNSDGTLMPLGIELTRPPMD 413
           +A+  KRLF++DYHD  +PYV +VR++  TTLYGSRT+FFL+ +GTLMPL IELTRP   
Sbjct: 456 EAMEKKRLFLLDYHDVFLPYVHRVRELPDTTLYGSRTVFFLSDEGTLMPLAIELTRPQSP 515

Query: 414 GHLEQWKEVFTPGTNSTDVWLWRLAKAHVLSHDSCIHQLVIHWLRAHCCMEPYAIATNRQ 473
               QWK  FT G+++T+ WLW+LAKAHVL+HD+  HQLV HWLR H C+EPY IATNRQ
Sbjct: 516 TK-PQWKRAFTHGSDATESWLWKLAKAHVLTHDTGYHQLVSHWLRTHACVEPYIIATNRQ 575

Query: 474 LSTMHPIYRLLHPHFRYNMRINANARKNLINAGGIIENAFSGASYSVELSSSAY-KAWRF 533
           LS MHP+YRLLHPHFRY M INA AR+ LINA GIIE AF    YS+ELSS AY  AW+F
Sbjct: 576 LSRMHPVYRLLHPHFRYTMEINALAREALINADGIIEEAFLAGKYSIELSSVAYGAAWQF 635

Query: 534 DEQALPEDLIHRGMAERKRDSEGRDVLELTIKDYPFANDGLLLWNALLEWVTEYVNHYYG 593
           + +ALPEDLI+RG+A R+ D E    LEL IKDYP+A+DGLL+W ++ +W ++YV+ YY 
Sbjct: 636 NTEALPEDLINRGLAVRRDDGE----LELAIKDYPYADDGLLIWGSIKQWASDYVDFYYK 695

Query: 594 DEENAVTNDKELQAWWGEIQAKGHPDKREG--WPALRTRDDLIKIASTIAWVGSGHHASV 653
            + + V  D+EL+AWW E++ KGH DK++   WP   T+++L++I + I WV SGHHA+V
Sbjct: 696 SDGD-VAGDEELRAWWEEVRTKGHADKKDEPWWPVCDTKENLVQILTIIMWVTSGHHAAV 755

Query: 654 NFLQYAYGGYMPNRPTIARTNMLTENHSDEILKDFRNYPENKLNELFPSVVQAAIVKQTM 713
           NF QY Y GY PNRPT+ R N+  E + D+ +K F   PE  L +  PS +QA  V  T+
Sbjct: 756 NFGQYHYAGYFPNRPTVVRRNIPVEENRDDEMKKFMARPEEVLLQSLPSQMQAIKVMATL 815

Query: 714 FLLSIHSPDEEYIGDAIEPAWALDHSISKAFEKFKAKLTDLEKKIDELNENKDLKNRYGA 773
            +LS HSPDEEY+G+  EPAW  +  +  AFEKF  +L + E  ID  N N + KNR GA
Sbjct: 816 DILSSHSPDEEYMGEYAEPAWLAEPMVKAAFEKFSGRLKEAEGTIDMRNNNPENKNRCGA 875

Query: 774 GIIPYEVMKPRSKPGVTGIGVPYSVSI 798
           GI+PYE++KP S+PGVTG G+P S+SI
Sbjct: 876 GIVPYELLKPFSEPGVTGRGIPNSISI 896

BLAST of CSPI04G13500.1 vs. TrEMBL
Match: S4UL90_MALDO (Lipoxygenase OS=Malus domestica GN=LOX6a PE=2 SV=1)

HSP 1 Score: 731.5 bits (1887), Expect = 1.1e-207
Identity = 407/780 (52.18%), Postives = 500/780 (64.10%), Query Frame = 1

Query: 95  YKGDIKVAEGFGKIGAVIVELQDGVNERFVDTIYVEAEKPPISVTFSCKSWVQPKGLIDH 154
           Y+ DIKV   FG+IGA++VE  +   E F+  I V+   P  SV  SC SW+  K     
Sbjct: 180 YETDIKVPVDFGEIGAILVE-NEHHKEMFLKEIVVDG-LPCGSVHHSCNSWIHSKYDNPE 239

Query: 155 QRIFFSSNKSYLSGKTPGGLVKLREEDLANLRGEKADGSVDRNERKAFERIYDYDLYNDL 214
           +R+FF+ NKSYL  +TP GLV+LREE+L  LRG        + ERK+FERIYDYD+YNDL
Sbjct: 240 KRVFFT-NKSYLPSQTPSGLVRLREEELLTLRGN------GQGERKSFERIYDYDVYNDL 299

Query: 215 GDPDQSMNLKRPVLG-------------------DPASEKRIEEWFYVPRDEEFSEIKQS 274
           GDPD+++ L+RPVLG                   DP SEKR  + +YVPRDE FSE+KQ 
Sbjct: 300 GDPDKNLRLQRPVLGGKEFPHPRRCRTGRPPCDIDPLSEKRSRKHWYVPRDEAFSEVKQL 359

Query: 275 SSQPGNEKLLRKSPFSDLPELEIKTPSAASKFNLQFNIASILSS------HRP------- 334
           +    + K L     + +P LE+    A +  NL F   + + S      H P       
Sbjct: 360 TF---SAKTLYSVMHALVPSLEM----AIADTNLGFKYFTAIDSLFHEGIHLPPFKEQGV 419

Query: 335 -----PALKSSDTPSSTLVELPPPESYKRDKYNWLSDIEFARLTLAGLNPYSIQLV---- 394
                P L +       ++   PPE+  RDK+ W  D EF R TLAGLNPYSI+LV    
Sbjct: 420 LKALLPRLVNVMATGDDVLRFVPPETMNRDKFFWFRDEEFGRQTLAGLNPYSIKLVTEWP 479

Query: 395 -KALADKRL--------------------------------FVVDYHDTLMPYVSKVRKI 454
            K+  D  +                                F++DYHD  +PYVSKVRK+
Sbjct: 480 LKSELDPAIYGPPESAITNEIIEREIGGFATITEAIREKKLFILDYHDLFLPYVSKVRKL 539

Query: 455 KGTTLYGSRTLFFLNSDGTLMPLGIELTRPPMDGHLEQWKEVFTPGTNSTDVWLWRLAKA 514
           +GTTLYGSRTLFFL  +GTL PL IELTRPPMDG   QWK+VF P  NSTDVWLWRLAKA
Sbjct: 540 EGTTLYGSRTLFFLTPEGTLRPLVIELTRPPMDGK-PQWKQVFQPAWNSTDVWLWRLAKA 599

Query: 515 HVLSHDSCIHQLVIHWLRAHCCMEPYAIATNRQLSTMHPIYRLLHPHFRYNMRINANARK 574
           HVL+HDS  HQLV HWLR HC  EPY IATNRQLS MHPIYRLLHPHFRY M IN+ AR 
Sbjct: 600 HVLAHDSGYHQLVSHWLRTHCATEPYIIATNRQLSVMHPIYRLLHPHFRYTMEINSLARD 659

Query: 575 NLINAGGIIENAFSGASYSVELSSSAY-KAWRFDEQALPEDLIHRGMAERKRDSEGRDVL 634
           +LINA GIIE +FS   YS+EL S AY K WRFD++ALP DLI RGMA    D      L
Sbjct: 660 SLINADGIIETSFSPGKYSLELCSIAYGKEWRFDQEALPADLIRRGMA--VEDPTAPHGL 719

Query: 635 ELTIKDYPFANDGLLLWNALLEWVTEYVNHYYGDEENAVTNDKELQAWWGEIQAKGHPDK 694
            LTI+DYPFANDGLLLW+A+ +WVT+YVNHYY D    V  D+ELQAWW EI+  GH DK
Sbjct: 720 RLTIEDYPFANDGLLLWDAIKQWVTDYVNHYYPD-SILVQTDEELQAWWTEIKTVGHADK 779

Query: 695 RE--GWPALRTRDDLIKIASTIAWVGSGHHASVNFLQYAYGGYMPNRPTIARTNMLTENH 754
           ++   WP L T +DL+ I +T+ WV SGHHA+VNF QYAYGGY PNRPT+ARTN+ TE+ 
Sbjct: 780 KDEPWWPELNTPEDLMGIITTMVWVASGHHAAVNFGQYAYGGYFPNRPTVARTNVPTEDP 839

Query: 755 SDEILKDFRNYPENKLNELFPSVVQAAIVKQTMFLLSIHSPDEEYIGDAIEPAWALDHSI 798
           S+E  K+F   PE+ L + FPS +QA  +   + +LS HSPDEEYIG+ +E AWA +  I
Sbjct: 840 SEEDWKNFIKKPESALLQCFPSQIQATRIMAVLDILSNHSPDEEYIGEKMEQAWAEEPVI 899

BLAST of CSPI04G13500.1 vs. TrEMBL
Match: M5W1M2_PRUPE (Lipoxygenase OS=Prunus persica GN=PRUPE_ppa000968mg PE=3 SV=1)

HSP 1 Score: 711.1 bits (1834), Expect = 1.6e-201
Identity = 398/776 (51.29%), Postives = 491/776 (63.27%), Query Frame = 1

Query: 95  YKGDIKVAEGFGKIGAVIVELQDGVNERFVDTIYVEAEKPPISVTFSCKSWVQPKGLIDH 154
           Y+ D KV   FG++GAV+VE  +   E F+  I ++   P  SV  SC SWV  K     
Sbjct: 189 YETDFKVPVHFGEVGAVLVE-NEHHKEMFLKDIVLDG-LPYGSVHLSCNSWVHSKYDNPE 248

Query: 155 QRIFFSSNKSYLSGKTPGGLVKLREEDLANLRGEKADGSVDRNERKAFERIYDYDLYNDL 214
           +R+FF+ NKSYL  +TP GLV+LREE+L  LRG        + ERK FERIYDYD+YNDL
Sbjct: 249 KRVFFT-NKSYLPSQTPNGLVRLREEELVTLRGN------GQGERKFFERIYDYDVYNDL 308

Query: 215 GDPDQSMNLKRPVLG-------------------DPASEKRIEEWFYVPRDEEFSEIKQS 274
           G+PD+++ L+RPVLG                   D  SEKR  +W YVPRDE FSE+KQ 
Sbjct: 309 GEPDKNLRLERPVLGGIEFPYPRRCRTGRPQCETDSLSEKRSNKW-YVPRDEAFSEVKQL 368

Query: 275 SSQPGNEKLLRKSPFSDLPELEIKT----------PSAASKFNLQFNIASI----LSSHR 334
           +    + K +     + +P LE              +  S FN   N+       +    
Sbjct: 369 TF---SAKTVYSVMHALVPSLETAMADNDHGFAYFTAIDSLFNEGINLPPFKEQGILKTL 428

Query: 335 PPALKSSDTPSSTLVELPPPESYKRDKYNWLSDIEFARLTLAGLNPYSIQLV-------- 394
            P L +       ++   PPE+  RDK+ W  D EFAR TLAGLNP S++LV        
Sbjct: 429 LPRLVNVVASGDDVLRFVPPETMNRDKFFWFRDEEFARQTLAGLNPCSLKLVTKWPLKSE 488

Query: 395 --------------KALADKR---------------LFVVDYHDTLMPYVSKVRKIKGTT 454
                         K + ++                LF++DYHD  +PYVSKVRKI+GTT
Sbjct: 489 LDPEIYGPPESAITKEIIEQEIRGFPTVQEAIREKKLFILDYHDLFLPYVSKVRKIEGTT 548

Query: 455 LYGSRTLFFLNSDGTLMPLGIELTRPPMDGHLEQWKEVFTPGTNSTDVWLWRLAKAHVLS 514
           LYGSRTLFFL  +GTL PL IELTRPPMDG   QWK+VF P  N+T VWLWRLAKAHVL+
Sbjct: 549 LYGSRTLFFLTREGTLRPLAIELTRPPMDGK-PQWKQVFQPSWNATGVWLWRLAKAHVLA 608

Query: 515 HDSCIHQLVIHWLRAHCCMEPYAIATNRQLSTMHPIYRLLHPHFRYNMRINANARKNLIN 574
           HDS  HQLV HWLR HC  EPY IAT+RQLS MHPIYR LHPHFRY M INA AR++LIN
Sbjct: 609 HDSGYHQLVSHWLRTHCVTEPYIIATSRQLSVMHPIYRFLHPHFRYTMEINALARESLIN 668

Query: 575 AGGIIENAFSGASYSVELSSSAY-KAWRFDEQALPEDLIHRGMAERKRDSEGRDVLELTI 634
           AGGIIE++FS   YS+ELS+ AY K WRFD++ALP DLI RGMA    D      L+LTI
Sbjct: 669 AGGIIESSFSPGKYSLELSAVAYGKEWRFDQEALPADLIRRGMA--VEDPTAPHGLKLTI 728

Query: 635 KDYPFANDGLLLWNALLEWVTEYVNHYYGDEENAVTNDKELQAWWGEIQAKGHPDKRE-- 694
           +DYPFANDGLL+W+A+ +WVT+YVNHYY D  + V  D ELQAWW EI+  GH DK++  
Sbjct: 729 EDYPFANDGLLMWDAIKQWVTDYVNHYYPD-SSLVQTDGELQAWWTEIKTVGHADKKDEP 788

Query: 695 GWPALRTRDDLIKIASTIAWVGSGHHASVNFLQYAYGGYMPNRPTIARTNMLTENHSDEI 754
            WP L T +DLI I +T+ WV SGHHA+VNF QY Y GY PNRPTIARTNM TE+ S E 
Sbjct: 789 WWPELNTPEDLIGIITTMVWVTSGHHAAVNFGQYVYAGYFPNRPTIARTNMPTEDPSPEF 848

Query: 755 LKDFRNYPENKLNELFPSVVQAAIVKQTMFLLSIHSPDEEYIGDAIEPAWALDHSISKAF 798
            K F   PE  L   FPS +QA  +   + +LS HSPDEEYIG+ +E AWA +  I  AF
Sbjct: 849 WKSFLKKPEVALLRCFPSQIQATRIMAVLDILSNHSPDEEYIGEKMEAAWAEEPVIKAAF 908

BLAST of CSPI04G13500.1 vs. TrEMBL
Match: A0A125S6K8_CUCME (Lipoxygenase OS=Cucumis melo var. makuwa GN=LOX13 PE=2 SV=1)

HSP 1 Score: 709.5 bits (1830), Expect = 4.5e-201
Identity = 414/849 (48.76%), Postives = 512/849 (60.31%), Query Frame = 1

Query: 39  TVVVTVIPKK-----------NEQFPWVEIIYLKFASVELD---SNEQPKEFIKCEAQLQ 98
           TV+  VI KK               P  +++ L FAS+ LD    +E+P   I  +A+L 
Sbjct: 78  TVITKVIVKKITGETTSSNLMEASQPPQKLLQLGFASILLDPRTGSEKPP--ITVQAKLI 137

Query: 99  EVDESVEYKKYKGDIKVAEGFGKIGAVIVELQDGVNERFVDTIYVEA-EKPPISVTFSCK 158
             DE  E   Y+  ++V   FG+IGAVIVE      E F+  +++      P+++  SC 
Sbjct: 138 SEDEVEEI--YEASLEVRSDFGEIGAVIVE-HHNEKEMFIKEVHLNGLTSGPLNI--SCN 197

Query: 159 SWVQPKGLIDHQRIFFSSNKSYLSGKTPGGLVKLREEDLANLRGEKADGSVDRNERKAFE 218
           SWVQPK L+  Q+  F +NKSYL  +TP GL  +RE +L NLRG          ER++++
Sbjct: 198 SWVQPKTLVPTQKRVFFTNKSYLPSQTPAGLKSMRETELMNLRGNGT------GERQSYD 257

Query: 219 RIYDYDLYNDLGDPDQSMNLKRPVLG--------------------DPASEKRIEEWFYV 278
           RIYDYD+YNDLGDPD+S +LKR +LG                    DP SE+R  E  YV
Sbjct: 258 RIYDYDVYNDLGDPDKSEDLKRSILGGPDNSPYPRRCRTGRPATKTDPNSEQRATESIYV 317

Query: 279 PRDEEFSEIKQSSSQPGNEKLLRKSPFSDLPELEIKTPSAASKFNLQ----------FNI 338
           PRDE FS+IK   S+  N   L  +  + +P L++     A   N +          FN+
Sbjct: 318 PRDEAFSDIK---SKAFNANQLSAALQTLIPRLQVHFDPNAGFPNFKAIDALFDVDGFNL 377

Query: 339 A---SILSSHR---PPALKSSDTPSSTLVELPPPESYKRDKYNWLSDIEFARLTLAGLNP 398
           +   S +SS +   P   K        L    PP    RDK+ WL D EFAR TLAG NP
Sbjct: 378 SPPDSSISSFKDLLPWIFKLIYETGEFLFRFKPPVPMDRDKFFWLRDEEFARQTLAGPNP 437

Query: 399 YSIQLVK------------------------------------ALADKRLFVVDYHDTLM 458
            SIQLVK                                    A+  K+L+++DYHD L+
Sbjct: 438 CSIQLVKEWPLSSQLDPKVYGPPESAFNTQMIDQEIGSMTVHEAIQKKKLYMLDYHDMLL 497

Query: 459 PYVSKVRKIKGTTLYGSRTLFFLNSDGTLMPLGIELTRPPMDGHLEQWKEVFTPGTNSTD 518
           PYV KVR +KGTTLYGSRTLFFLN D TL PL IELTRPPM+G   QWK V+ P   +T 
Sbjct: 498 PYVQKVRALKGTTLYGSRTLFFLNEDETLRPLAIELTRPPMNGK-PQWKRVYGPSEKATS 557

Query: 519 VWLWRLAKAHVLSHDSCIHQLVIHWLRAHCCMEPYAIATNRQLSTMHPIYRLLHPHFRYN 578
           +WLWR AKAHVL+HD+  HQLV HWLR HC +EPY IATNRQLS MHPIYRLLHPHFRY 
Sbjct: 558 LWLWRFAKAHVLAHDAGYHQLVSHWLRTHCAVEPYVIATNRQLSAMHPIYRLLHPHFRYT 617

Query: 579 MRINANARKNLINAGGIIENAFSGASYSVELSSSAYK-AWRFDEQALPEDLIHRGMAERK 638
           M INA+AR NLIN GG IE+ FS   YS+ELSS+AY   W+FD QALP DLIHRG+AE  
Sbjct: 618 MEINASARTNLINGGGTIESTFSPLKYSMELSSTAYDLQWQFDLQALPADLIHRGLAE-- 677

Query: 639 RDSEGRDVLELTIKDYPFANDGLLLWNALLEWVTEYVNHYYGDEENAVTNDKELQAWWGE 698
            D      L+L IKDYPFANDGL+LW+AL +WVTEYVNHYY D    VT D ELQAWW E
Sbjct: 678 EDPTASHGLKLHIKDYPFANDGLILWDALKQWVTEYVNHYYPD-PYLVTTDTELQAWWTE 737

Query: 699 IQAKGHPDKRE--GWPALRTRDDLIKIASTIAWVGSGHHASVNFLQYAYGGYMPNRPTIA 758
           I+  GH DK+E   WP L T  DLI I + IAWV S HHA+VNF QYAY GY PNRP+I 
Sbjct: 738 IRTVGHADKQEKPWWPLLNTPQDLIDIVTNIAWVASAHHAAVNFGQYAYSGYFPNRPSIT 797

Query: 759 RTNMLTENHSDEILKDFRNYPENKLNELFPSVVQAAIVKQTMFLLSIHSPDEEYIGDAIE 798
           RTNM TE+++  + K F   PE  L + FPS  QA  V   M +LS HSPDEEYIG  +E
Sbjct: 798 RTNMPTEDNNPALWKSFLEKPEELLLDSFPSQFQATQVMLVMNMLSSHSPDEEYIGKDME 857

BLAST of CSPI04G13500.1 vs. TrEMBL
Match: S4UKU6_MALDO (Lipoxygenase OS=Malus domestica GN=LOX6b PE=2 SV=1)

HSP 1 Score: 702.2 bits (1811), Expect = 7.2e-199
Identity = 405/815 (49.69%), Postives = 505/815 (61.96%), Query Frame = 1

Query: 58  IYLKFASVELDSNE-QPKEFIKCEAQLQEVDESVEYKKYKGDIKVAEGFGKIGAVIVELQ 117
           + L+  S ELD    + KE +   A      E      Y+ D KV   FG+IGA++VE  
Sbjct: 143 LLLELVSAELDPKTGEEKEKVAGYAHRSRRQEGEII--YETDFKVPVDFGEIGAILVE-N 202

Query: 118 DGVNERFVDTIYVEAEKPPISVTFSCKSWVQPKGLIDHQRIFFSSNKSYLSGKTPGGLVK 177
           +   E F+  I V+   P  SV  SC SW+  K     +R+FF+ NKSYL  +TP GL K
Sbjct: 203 EHRKEMFLKEIVVDG-LPCGSVHHSCNSWIHSKYDNPAKRVFFT-NKSYLPSQTPSGLAK 262

Query: 178 LREEDLANLRGEKADGSVDRNERKAFERIYDYDLYNDLGDPDQSMNLKRPVLG------- 237
           LREE+L  LRG        + ERK+FERIYDYD+YNDLGDPD+++ L+RPVLG       
Sbjct: 263 LREEELVTLRGN------GQGERKSFERIYDYDVYNDLGDPDKNLRLERPVLGGKEFPYP 322

Query: 238 ----------DPAS--EKRIEEWFYVPRDEEFSEIKQSSSQPGNEKLLRKSPFSDLPELE 297
                     D  S  EKR  + +YVPRDE FSE+KQ +    + K L     + +P LE
Sbjct: 323 RRCRTGRLPCDTGSLYEKRSRKHWYVPRDEAFSEVKQLTF---SAKTLYSVMHALVPSLE 382

Query: 298 IKTPSAASKFNL---------------QFNIASILSSHRPPALKSSDTPSSTLVELPPPE 357
           I        F                  F    +L +  P  +K   +    L    PPE
Sbjct: 383 IAIADTDLGFKYFTAIDSLFHEGIQLPPFKEQGVLKALLPRLVKVMSSGDDVL-RFVPPE 442

Query: 358 SYKRDKYNWLSDIEFARLTLAGLNPYSIQLV-----KALADKRLF----------VVDYH 417
           +  RDK+ W  D EFAR TLAGLNPYSI+LV     K+  D  ++          +++  
Sbjct: 443 TMNRDKFFWFRDDEFARQTLAGLNPYSIKLVTEWPLKSELDPEIYGPPESAITKEIIEQE 502

Query: 418 DT----------------------LMPYVSKVRKIKGTTLYGSRTLFFLNSDGTLMPLGI 477
                                    +PYVSKVRK++GTTLYGSRTLFFL  +GTL PL I
Sbjct: 503 IRGFATITEAIEEKKLFILDYHDLFLPYVSKVRKLEGTTLYGSRTLFFLTPEGTLRPLVI 562

Query: 478 ELTRPPMDGHLEQWKEVFTPGTNSTDVWLWRLAKAHVLSHDSCIHQLVIHWLRAHCCMEP 537
           ELTRPPMDG   QWK+VF P  N+TDVWLWRLAKAHVL+HDS  HQLV HWLR HC  EP
Sbjct: 563 ELTRPPMDGK-PQWKQVFQPAWNATDVWLWRLAKAHVLAHDSGYHQLVSHWLRTHCATEP 622

Query: 538 YAIATNRQLSTMHPIYRLLHPHFRYNMRINANARKNLINAGGIIENAFSGASYSVELSSS 597
           Y IATNRQLS MHPIYRLLHPHFRY M IN+ AR++LINAGGIIE +FS   YS+EL S 
Sbjct: 623 YIIATNRQLSVMHPIYRLLHPHFRYTMEINSLARESLINAGGIIETSFSPKKYSLELCSV 682

Query: 598 AY-KAWRFDEQALPEDLIHRGMAERKRDSEGRDVLELTIKDYPFANDGLLLWNALLEWVT 657
           AY K WRFD++ALP DLI RGMA    D      L LTI+DYPFANDGLLLW+A+ +WVT
Sbjct: 683 AYGKEWRFDQEALPADLIRRGMA--VEDPTAPHGLRLTIEDYPFANDGLLLWDAIKQWVT 742

Query: 658 EYVNHYYGDEENAVTNDKELQAWWGEIQAKGHPDKRE--GWPALRTRDDLIKIASTIAWV 717
           +YVNHYY D  + V  D+ELQAWW EI+  GH DK++   WP L T +DL+ I +T+ WV
Sbjct: 743 DYVNHYYPD-SSIVQTDQELQAWWTEIKTVGHADKKDEPWWPELNTPEDLMGIITTMVWV 802

Query: 718 GSGHHASVNFLQYAYGGYMPNRPTIARTNMLTENHSDEILKDFRNYPENKLNELFPSVVQ 777
            SGHHA+VNF QYAY GY P+RPTIARTNM TE+ S+E  K+F   P++ L + FP+ +Q
Sbjct: 803 ASGHHAAVNFGQYAYAGYFPSRPTIARTNMPTEDPSEEDWKNFVRKPDSALLQCFPTQIQ 862

Query: 778 AAIVKQTMFLLSIHSPDEEYIGDAIEPAWALDHSISKAFEKFKAKLTDLEKKIDELNENK 798
           A  +   + +LS HSPDEEYIG+ +E AWA +  I  AFE+FK +L  LE+ ID+ N + 
Sbjct: 863 ATTIMAVLDILSNHSPDEEYIGEKMEQAWAEEPVIKAAFERFKGRLLALERTIDDRNASS 922

BLAST of CSPI04G13500.1 vs. TrEMBL
Match: S4UKZ5_MALDO (Lipoxygenase OS=Malus domestica GN=LOX6b PE=2 SV=1)

HSP 1 Score: 700.7 bits (1807), Expect = 2.1e-198
Identity = 404/815 (49.57%), Postives = 504/815 (61.84%), Query Frame = 1

Query: 58  IYLKFASVELDSNE-QPKEFIKCEAQLQEVDESVEYKKYKGDIKVAEGFGKIGAVIVELQ 117
           + L+  S ELD    + KE +   A      E      Y+ D KV   FG+IGA++VE  
Sbjct: 143 LLLELVSAELDPKTGEEKEKVAGYAHRSRRQEGEII--YETDFKVPVDFGEIGAILVE-N 202

Query: 118 DGVNERFVDTIYVEAEKPPISVTFSCKSWVQPKGLIDHQRIFFSSNKSYLSGKTPGGLVK 177
           +   E F+  I V+   P  SV  SC SW+  K     +R+FF+ NKSYL  +TP GL K
Sbjct: 203 EHRKEMFLKEIVVDG-LPCGSVHHSCNSWIHSKYDNPAKRVFFT-NKSYLPSQTPSGLAK 262

Query: 178 LREEDLANLRGEKADGSVDRNERKAFERIYDYDLYNDLGDPDQSMNLKRPVLG------- 237
           LREE+L  LRG        + ERK+FERIYDYD+YNDLGDPD+++ L+RPVLG       
Sbjct: 263 LREEELVTLRGN------GQGERKSFERIYDYDVYNDLGDPDKNLRLERPVLGGKEFPYP 322

Query: 238 ----------DPAS--EKRIEEWFYVPRDEEFSEIKQSSSQPGNEKLLRKSPFSDLPELE 297
                     D  S  EKR  + +YVPRDE FSE+KQ +    + K L     + +P LE
Sbjct: 323 RRCRTGRLPCDTGSLYEKRSRKHWYVPRDEAFSEVKQLTF---SAKTLYSVMHALVPSLE 382

Query: 298 IKTPSAASKFNL---------------QFNIASILSSHRPPALKSSDTPSSTLVELPPPE 357
           I        F                  F    +L +  P  +K   +    L    PPE
Sbjct: 383 IAIADTDLGFKYFTAIDSLFHEGIQLPPFKEQGVLKALLPRLVKVMSSGDDVL-RFVPPE 442

Query: 358 SYKRDKYNWLSDIEFARLTLAGLNPYSIQLV-----KALADKRLF----------VVDYH 417
           +  RDK+ W  D EFAR TLAGLNPYSI+LV     K+  D  ++          +++  
Sbjct: 443 TMNRDKFFWFRDDEFARQTLAGLNPYSIKLVTEWPLKSELDPEIYGPPESAITKEIIEQE 502

Query: 418 DT----------------------LMPYVSKVRKIKGTTLYGSRTLFFLNSDGTLMPLGI 477
                                    +PYVSKVRK++GTTLYGSRTLFFL  +GTL PL I
Sbjct: 503 IRGFATITEAIEEKKLFILDYHDLFLPYVSKVRKLEGTTLYGSRTLFFLTPEGTLRPLVI 562

Query: 478 ELTRPPMDGHLEQWKEVFTPGTNSTDVWLWRLAKAHVLSHDSCIHQLVIHWLRAHCCMEP 537
           ELTRPPMDG   QWK+VF P  N+TDVWLWRLAKAHVL+HDS  HQLV HWLR HC  EP
Sbjct: 563 ELTRPPMDGK-PQWKQVFQPAWNATDVWLWRLAKAHVLAHDSGYHQLVSHWLRTHCATEP 622

Query: 538 YAIATNRQLSTMHPIYRLLHPHFRYNMRINANARKNLINAGGIIENAFSGASYSVELSSS 597
           Y IATNRQLS MHPIYRLLHPHFRY M IN+ AR++LINAGGIIE +FS   YS+EL S 
Sbjct: 623 YIIATNRQLSVMHPIYRLLHPHFRYTMEINSLARESLINAGGIIETSFSPKKYSLELCSV 682

Query: 598 AY-KAWRFDEQALPEDLIHRGMAERKRDSEGRDVLELTIKDYPFANDGLLLWNALLEWVT 657
            Y K WRFD++ALP DLI RGMA    D      L LTI+DYPFANDGLLLW+A+ +WVT
Sbjct: 683 VYGKEWRFDQEALPADLIRRGMA--VEDPTAPHGLRLTIEDYPFANDGLLLWDAIKQWVT 742

Query: 658 EYVNHYYGDEENAVTNDKELQAWWGEIQAKGHPDKRE--GWPALRTRDDLIKIASTIAWV 717
           +YVNHYY D  + V  D+ELQAWW EI+  GH DK++   WP L T +DL+ I +T+ WV
Sbjct: 743 DYVNHYYPD-SSIVQTDQELQAWWTEIKTVGHADKKDEPWWPELNTPEDLMGIITTMVWV 802

Query: 718 GSGHHASVNFLQYAYGGYMPNRPTIARTNMLTENHSDEILKDFRNYPENKLNELFPSVVQ 777
            SGHHA+VNF QYAY GY P+RPTIARTNM TE+ S+E  K+F   P++ L + FP+ +Q
Sbjct: 803 ASGHHAAVNFGQYAYAGYFPSRPTIARTNMPTEDPSEEDWKNFVRKPDSALLQCFPTQIQ 862

Query: 778 AAIVKQTMFLLSIHSPDEEYIGDAIEPAWALDHSISKAFEKFKAKLTDLEKKIDELNENK 798
           A  +   + +LS HSPDEEYIG+ +E AWA +  I  AFE+FK +L  LE+ ID+ N + 
Sbjct: 863 ATTIMAVLDILSNHSPDEEYIGEKMEQAWAEEPVIKAAFERFKGRLLALERTIDDRNASS 922

BLAST of CSPI04G13500.1 vs. TAIR10
Match: AT3G45140.1 (AT3G45140.1 lipoxygenase 2)

HSP 1 Score: 655.2 bits (1689), Expect = 5.1e-188
Identity = 371/801 (46.32%), Postives = 475/801 (59.30%), Query Frame = 1

Query: 77  IKCEAQLQEVDESVEYKKYKGDIKVAEGFGKIGAVIVELQDGVNERFVDTIYVEAEKPPI 136
           I  E   Q V      +KY+ + ++ E FG +GA+ ++ Q     R +    VE + P  
Sbjct: 119 ITVEDYAQRVWAEAPDEKYECEFEMPEDFGPVGAIKIQNQ---YHRQLFLKGVELKLPGG 178

Query: 137 SVTFSCKSWVQPKGLIDHQRIFFSSNKSYLSGKTPGGLVKLREEDLANLRGEKADGSVDR 196
           S+TF+C+SWV PK +   +RIFFS +KSYL  +TP  L K R+E+L  L+G+  +   + 
Sbjct: 179 SITFTCESWVAPKSVDPTKRIFFS-DKSYLPSQTPEPLKKYRKEELETLQGKNRE---EV 238

Query: 197 NERKAFERIYDYDLYNDLGDPDQSMNLKRPVLG-------------------DPASEKRI 256
            E   FERIYDYD+YND+GDPD    L RPV+G                   DP+SE+R 
Sbjct: 239 GEFTKFERIYDYDVYNDVGDPDNDPELARPVIGGLTHPYPRRCKTGRKPCETDPSSEQRY 298

Query: 257 EEWFYVPRD---------------------EEFSEIKQSSSQPGNEKLLRKSPFSDLPEL 316
              FYVPRD                       F +I+     P  E         +L E 
Sbjct: 299 GGEFYVPRDEEFSTAKGTSFTGKAVLAALPSIFPQIESVLLSP-QEPFPHFKAIQNLFEE 358

Query: 317 EIKTPSAASKFNLQFNIASILSSHRPPALKSSDTPSSTLVELPPPESYKRDKYNWLSDIE 376
            I+ P  A    L            P  +K+       +++   P    RD+++WL D E
Sbjct: 359 GIQLPKDAGLLPLL-----------PRIIKALGEAQDDILQFDAPVLINRDRFSWLRDDE 418

Query: 377 FARLTLAGLNPYSIQLVK-----------ALADKRLFVVD-------------------- 436
           FAR TLAGLNPYSIQLV+              D    +                      
Sbjct: 419 FARQTLAGLNPYSIQLVEEWPLISKLDPAVYGDPTSLITWEIVEREVKGNMTVDEALKNK 478

Query: 437 ------YHDTLMPYVSKVRKIKGTTLYGSRTLFFLNSDGTLMPLGIELTRPPMDGHLEQW 496
                 YHD L+PYV+KVR++  TTLY SRTLFFL+ D TL P+ IELT PP + +  QW
Sbjct: 479 RLFVLDYHDLLLPYVNKVRELNNTTLYASRTLFFLSDDSTLRPVAIELTCPP-NINKPQW 538

Query: 497 KEVFTPGTNSTDVWLWRLAKAHVLSHDSCIHQLVIHWLRAHCCMEPYAIATNRQLSTMHP 556
           K+VFTPG ++T  WLW LAK H +SHD+  HQL+ HWLR H C EPY IA NRQLS MHP
Sbjct: 539 KQVFTPGYDATSCWLWNLAKTHAISHDAGYHQLISHWLRTHACTEPYIIAANRQLSAMHP 598

Query: 557 IYRLLHPHFRYNMRINANARKNLINAGGIIENAFSGASYSVELSSSAY-KAWRFDEQALP 616
           IYRLLHPHFRY M INA AR++L+N GGIIE  F    Y++ELSS+ Y K WRFD++ LP
Sbjct: 599 IYRLLHPHFRYTMEINARARQSLVNGGGIIETCFWPGKYALELSSAVYGKLWRFDQEGLP 658

Query: 617 EDLIHRGMAERKRDSEGRDVLELTIKDYPFANDGLLLWNALLEWVTEYVNHYYGDEENAV 676
            DLI RG+AE  + +E    + LTI DYPFANDGL+LW+A+ EWVT+YV HYY DEE  +
Sbjct: 659 ADLIKRGLAEEDKTAE--HGVRLTIPDYPFANDGLILWDAIKEWVTDYVKHYYPDEE-LI 718

Query: 677 TNDKELQAWWGEIQAKGHPDKRE--GWPALRTRDDLIKIASTIAWVGSGHHASVNFLQYA 736
           T+D+ELQ WW E++  GH DK++   WP L+T+DDLI + +TIAWV SGHHA+VNF QY 
Sbjct: 719 TSDEELQGWWSEVRNIGHGDKKDEPWWPVLKTQDDLIGVVTTIAWVTSGHHAAVNFGQYG 778

Query: 737 YGGYMPNRPTIARTNMLTENHSDEILKDFRNYPENKLNELFPSVVQAAIVKQTMFLLSIH 796
           YGGY PNRPT  R  M TE+ +DE LK+F   PE  L + +PS  QA +V  T+ LLS H
Sbjct: 779 YGGYFPNRPTTTRIRMPTEDPTDEALKEFYESPEKVLLKTYPSQKQATLVMVTLDLLSTH 838

Query: 797 SPDEEYIGDAIEPAWALDHSISKAFEKFKAKLTDLEKKIDELNENKDLKNRYGAGIIPYE 798
           SPDEEYIG+  E +WA +  I+ AFE+FK KL  LE  IDE N N  LKNR GAG++ YE
Sbjct: 839 SPDEEYIGEQQEASWANEPVINAAFERFKGKLQYLEGVIDERNVNITLKNRAGAGVVKYE 896

BLAST of CSPI04G13500.1 vs. TAIR10
Match: AT1G67560.1 (AT1G67560.1 PLAT/LH2 domain-containing lipoxygenase family protein)

HSP 1 Score: 520.0 bits (1338), Expect = 2.6e-147
Identity = 336/866 (38.80%), Postives = 460/866 (53.12%), Query Frame = 1

Query: 32  ERMSYMGTVVVTVI--------PKKNEQFPWVEIIYLKFAS----VELDSNEQPKEFIKC 91
           +R  Y G+  VT +         K  E+F     +++K       ++L S E   E  K 
Sbjct: 72  QRSKYTGSKTVTAVVKIRKKIKEKLTERFEHQLELFMKAIGQGMLIQLVSEEIDPETGKG 131

Query: 92  EAQLQ-------EVDESVEYKKYKGDIKVAEGFGKIGAVIVELQDGVNERFVDTIYVEAE 151
              L+       +  +   Y  +  D  V   FGK GA++V       E  +  I +E  
Sbjct: 132 RKSLESPVMGLPKAVKDPRYLVFTADFTVPINFGKPGAILVTNLLST-EICLSEIIIEDS 191

Query: 152 KPPISVTFSCKSWVQPKGLIDHQRIFFSSNKSYLSGKTPGGLVKLREEDLANLRGEKADG 211
               ++ F   +W+  K      RI F S    L  +TP G+ +LRE+DL ++RG+    
Sbjct: 192 TD--TILFPANTWIHSKNDNPQARIIFRSQPC-LPSETPDGIKELREKDLVSVRGD---- 251

Query: 212 SVDRNERKAFERIYDYDLYNDLGDPDQSMNLKRPVLG-------------------DPAS 271
              + ERK  ERIYDYD+YNDLGDP ++  + RPVLG                   DP  
Sbjct: 252 --GKGERKPHERIYDYDVYNDLGDPRKTERV-RPVLGVPETPYPRRCRTGRPLVSKDPPC 311

Query: 272 EKR--IEEWFYVPRDEEFSEIKQSSSQPGNEKLLRK----SPFSDLPELEIKTPSAASKF 331
           E R   +E FYVPRDE F EIK+ + + G  K L      S  + L  L+I     +   
Sbjct: 312 ESRGKEKEEFYVPRDEVFEEIKRDTFRAGRFKALFHNLVPSIAAALSNLDIPFTCFSDID 371

Query: 332 NLQFNIASILSSHRPPA-----------LKSSDTPSSTLVELPPPESYKRDKYNWLSDIE 391
           NL  +  +I+  H  P            +      + TL++   P   K D++ WL D E
Sbjct: 372 NLYKS--NIVLGHTEPKDTGLGGFIGGFMNGILNVTETLLKYDTPAVIKWDRFAWLRDNE 431

Query: 392 FARLTLAGLNPYSIQLVKAL-----ADKRLFVVD-------------------------- 451
           F R  LAG+NP +I+L+K L      D  L+                             
Sbjct: 432 FGRQALAGVNPVNIELLKELPIRSNLDPALYGPQESVLTEEIIAREVEHYGTTIEKALEE 491

Query: 452 -------YHDTLMPYVSKVRKIK--GTTLYGSRTLFFLNSDGTLMPLGIELTRPPMDGHL 511
                  YHD L+P+V K+  IK      Y SRT+FF + +G L PL IEL+ PP     
Sbjct: 492 KRLFLVDYHDILLPFVEKINSIKEDPRKTYASRTIFFYSKNGALRPLAIELSLPPTAE-- 551

Query: 512 EQWKEVFTPGTNSTDVWLWRLAKAHVLSHDSCIHQLVIHWLRAHCCMEPYAIATNRQLST 571
            + K V+T G ++T  W+W+LAKAHV S+D+ +HQLV HWLR H  MEPY IATNRQLST
Sbjct: 552 SENKFVYTHGHDATTHWIWKLAKAHVCSNDAGVHQLVNHWLRTHASMEPYIIATNRQLST 611

Query: 572 MHPIYRLLHPHFRYNMRINANARKNLINAGGIIENAFSGASYSVELSSSAYKA-WRFDEQ 631
           MHP+Y+LLHPH RY + INA ARK+LIN GGIIE+ F+   Y++ELSS+AYK+ WRFD +
Sbjct: 612 MHPVYKLLHPHMRYTLEINARARKSLINGGGIIESCFTPGKYAMELSSAAYKSMWRFDME 671

Query: 632 ALPEDLIHRGMAERKRDSEGRDVLELTIKDYPFANDGLLLWNALLEWVTEYVNHYYGDEE 691
            LP DL+ RGMAE   DS     + L I DYP+A DGLL+W A+ + V  YV H+Y D +
Sbjct: 672 GLPADLVRRGMAE--EDSSAECGVRLVIDDYPYAADGLLIWKAIKDLVESYVKHFYSDSK 731

Query: 692 NAVTNDKELQAWWGEIQAKGHPDKRE--GWPALRTRDDLIKIASTIAWVGSGHHASVNFL 751
            ++T+D ELQAWW EI+ KGH DK++   WP L T  DL +I + + W+ SG HA++NF 
Sbjct: 732 -SITSDLELQAWWDEIKNKGHYDKKDEPWWPKLNTTQDLSQILTNMIWIASGQHAAINFG 791

Query: 752 QYAYGGYMPNRPTIARTNMLTENHSDEILKDFRNYPENKLNELFPSVVQAAIVKQTMFLL 798
           QY +GGY+PNRPT+ R   L    +D   + F   P+       P+ +QA  V      L
Sbjct: 792 QYPFGGYVPNRPTLLR--KLIPQETDPDYEMFMRNPQYSFLGSLPTQLQATKVMAVQETL 851

BLAST of CSPI04G13500.1 vs. TAIR10
Match: AT1G17420.1 (AT1G17420.1 lipoxygenase 3)

HSP 1 Score: 510.0 bits (1312), Expect = 2.7e-144
Identity = 310/780 (39.74%), Postives = 441/780 (56.54%), Query Frame = 1

Query: 95  YKGDIKVAEGFGKIGAVIVELQDGVNERFVDTIYVE--AEKPPISVTFSCKSWVQPKGLI 154
           Y  +  V   FG  GA+ V +     E F+++I +E  A  P   V F C SWVQ +   
Sbjct: 159 YTAEFTVDAAFGSPGAITV-MNKHQKEFFLESITIEGFALGP---VHFPCNSWVQSQKDH 218

Query: 155 DHQRIFFSSNKSYLSGKTPGGLVKLREEDLANLRGEKADGSVDRNERKAFERIYDYDLYN 214
             +RIFF+ N+ YL  +TP GL  LRE++L NLRG   DGS     RK  +RIYD+D+YN
Sbjct: 219 PDKRIFFT-NQPYLPNETPSGLRVLREKELKNLRG---DGS---GVRKLSDRIYDFDVYN 278

Query: 215 DLGDPDQSMNLKRPVLG-------------------DPASEKRIEEWF--YVPRDEEFSE 274
           DLG+PD+S  L RP LG                   D  +E R+E+    YVPRDE+F E
Sbjct: 279 DLGNPDKSSELSRPKLGGKEVPYPRRCRTGRQSTVSDKDAESRVEKPLPMYVPRDEQFEE 338

Query: 275 IKQ--------------------------------SSSQPGNEKLLRKSPFSD------- 334
            KQ                                   +   E LL K  F D       
Sbjct: 339 SKQDTFAAGRLKAVLHHLIPSLKASIVAEDFADFGEIDRLYKEGLLLKLGFQDDIFKKFP 398

Query: 335 LPELEIKTPSAASKFNLQFNIASILSSHRPPALKSSDTPSSTLVELPPPESYKRDKYNWL 394
           LP++ + T   ++K  L+++   ILS  +   L+  +     +  + P    +   +  +
Sbjct: 399 LPKVVVDTLQESTKGLLKYDTPKILSKDKNAWLRDDEFARQAIAGINPVNIERVKTFPPV 458

Query: 395 SDIE-------FARLT----LAGLNPYSIQLVKALADKRLFVVDYHDTLMPYVSKVRKIK 454
           S+++        + LT    +  L+ +S+Q  +AL + RL+++DYHD  +P++ ++  + 
Sbjct: 459 SNLDPKIYGPQHSALTDDHIIGHLDGFSVQ--QALEENRLYMLDYHDIFLPFLDRINALD 518

Query: 455 GTTLYGSRTLFFLNSDGTLMPLGIELTRPPMDGHLEQWKEVFTPGTNSTDVWLWRLAKAH 514
           G   Y +RT+FFL   GTL P+ IEL+ PP  G   + K V TP  ++T  W+W+LAKAH
Sbjct: 519 GRKAYATRTIFFLTRLGTLKPVAIELSLPP-HGPKHRSKRVLTPPVDATSNWMWQLAKAH 578

Query: 515 VLSHDSCIHQLVIHWLRAHCCMEPYAIATNRQLSTMHPIYRLLHPHFRYNMRINANARKN 574
           V S+D+ +HQLV HWLR H C+EP+ +A +RQLS MHPI++LL PH RY + INA AR++
Sbjct: 579 VSSNDAGVHQLVNHWLRTHACLEPFILAAHRQLSAMHPIFKLLDPHMRYTLEINALARQS 638

Query: 575 LINAGGIIENAFSGASYSVELSSSAYK-AWRFDEQALPEDLIHRGMAERKRDSEGRDVLE 634
           LI+A G+IE  F+  +Y +E+S++AYK +WRFD + LP DLI RGMA    D+     L+
Sbjct: 639 LISADGVIEGGFTAGAYGMEMSAAAYKSSWRFDMEGLPADLIRRGMA--IPDATQPHGLK 698

Query: 635 LTIKDYPFANDGLLLWNALLEWVTEYVNHYYGDEENAVTNDKELQAWWGEIQAKGHPDKR 694
           L I+DYP+ANDGLLLW+A+  WV  YV  YY    N +  D ELQ+W+ E    GH D R
Sbjct: 699 LLIEDYPYANDGLLLWSAIQTWVRTYVERYY-PNPNLIKTDSELQSWYSESINVGHADLR 758

Query: 695 EG--WPALRTRDDLIKIASTIAWVGSGHHASVNFLQYAYGGYMPNRPTIARTNMLTENHS 754
           +   WP L T DDL+ I +T+ W+ S  HA++NF QY YGGY+PNRP + R   L  + S
Sbjct: 759 DADWWPELSTVDDLVSILTTLIWLASAQHAALNFGQYPYGGYVPNRPPLMR--RLIPDES 818

Query: 755 DEILKDFRNYPENKLNELFPSVVQAAIVKQTMFLLSIHSPDEEYIGDAIEPA-WALDHSI 798
           D     F ++PE       PS+ Q +     +  LS HSPDEEYIG+  +P+ W  D  I
Sbjct: 819 DPEYASFISHPEKYYFSSMPSLAQTSKFMAVVDTLSTHSPDEEYIGERQQPSIWTGDAEI 878

BLAST of CSPI04G13500.1 vs. TAIR10
Match: AT1G72520.1 (AT1G72520.1 PLAT/LH2 domain-containing lipoxygenase family protein)

HSP 1 Score: 492.3 bits (1266), Expect = 5.7e-139
Identity = 304/787 (38.63%), Postives = 429/787 (54.51%), Query Frame = 1

Query: 87  DESVEYKKYKGDIKVAEGFGKIGAVIVELQDGVNERFVDTIYVEAEKPPISVTFSCKSWV 146
           +   E   Y  +  V   FG  GA+ V  +    E F+++I +E       V F C SWV
Sbjct: 157 NSKAERVHYTAEFTVDSAFGSPGAITVTNKHQ-KEFFLESITIEGFACG-PVHFPCNSWV 216

Query: 147 QPKGLIDHQRIFFSSNKSYLSGKTPGGLVKLREEDLANLRGEKADGSVDRNERKAFERIY 206
           Q +     +RI F+ N+ YL  +TP GL  LRE++L NLRG        + ERK  +RIY
Sbjct: 217 QSQKDHPSKRILFT-NQPYLPSETPSGLRTLREKELENLRGN------GKGERKLSDRIY 276

Query: 207 DYDLYNDLGDPDQSMNLKRPVLG-------------------DPASEKRIEEWF--YVPR 266
           DYD+YND+G+PD S  L RP LG                   D  SE+R+E+    YVPR
Sbjct: 277 DYDVYNDIGNPDISRELARPTLGGREFPYPRRCRTGRSSTDTDMMSERRVEKPLPMYVPR 336

Query: 267 DEE--------------------------FSEIKQSSSQPGN------EKLLRKSPFSD- 326
           DE+                           S + +  +  G       E LL K  F D 
Sbjct: 337 DEQFEESKQNTFAACRLKAVLHNLIPSLKASILAEDFANFGEIDSLYKEGLLLKLGFQDD 396

Query: 327 ------LPELEIKTPSAASKFNLQFNIASILSSHRPPALKSSDTPSSTLVELPPPESYKR 386
                 LP++ + T   +S+  L+++   I+S  +   L+  +     +  + P    + 
Sbjct: 397 MFKKFPLPKI-VTTLQKSSEGLLRYDTPKIVSKDKYAWLRDDEFARQAIAGINPVNIERV 456

Query: 387 DKYNWLSDIE--------FARLT----LAGLNPYSIQLVKALADKRLFVVDYHDTLMPYV 446
             Y  +S+++         + LT    +  L+  ++Q  +AL   RLF+VDYHD  +P++
Sbjct: 457 TSYPPVSNLDPEIYGPGLHSALTEDHIIGQLDGLTVQ--QALETNRLFMVDYHDIYLPFL 516

Query: 447 SKVRKIKGTTLYGSRTLFFLNSDGTLMPLGIELTRPPMDGHLEQWKEVFTPGTNSTDVWL 506
            ++  + G   Y +RT+ FL   GTL P+ IEL+ P      ++ K V TP  ++T  W+
Sbjct: 517 DRINALDGRKAYATRTILFLTRLGTLKPIAIELSLPSQSSSNQKSKRVVTPPVDATSNWM 576

Query: 507 WRLAKAHVLSHDSCIHQLVIHWLRAHCCMEPYAIATNRQLSTMHPIYRLLHPHFRYNMRI 566
           W+LAKAHV S+D+ +HQLV HWLR H C+EP+ +A +RQLS MHPI++LL PH RY + I
Sbjct: 577 WQLAKAHVGSNDAGVHQLVNHWLRTHACLEPFILAAHRQLSAMHPIFKLLDPHMRYTLEI 636

Query: 567 NANARKNLINAGGIIENAFSGASYSVELSSSAYK-AWRFDEQALPEDLIHRGMAERKRDS 626
           NA AR+ LI+A G+IE+ F+   Y +E+SS+AYK  WRFD + LP DLI RGMA    D 
Sbjct: 637 NAVARQTLISADGVIESCFTAGQYGLEISSAAYKNKWRFDMEGLPADLIRRGMA--VPDP 696

Query: 627 EGRDVLELTIKDYPFANDGLLLWNALLEWVTEYVNHYYGDEENAVTNDKELQAWWGEIQA 686
                L+L ++DYP+ANDGLLLW+A+  WV  YV  YY +  N +  D ELQAW+ E   
Sbjct: 697 TQPHGLKLLVEDYPYANDGLLLWSAIQTWVRTYVERYYAN-SNLIQTDTELQAWYSESIN 756

Query: 687 KGHPDKR--EGWPALRTRDDLIKIASTIAWVGSGHHASVNFLQYAYGGYMPNRPTIARTN 746
            GH D R  E WP L T +DL+ + +TI W+ S  HA++NF QY YGGY+PNRP + R  
Sbjct: 757 VGHADHRDAEWWPKLSTVEDLVSVITTIIWLASAQHAALNFGQYPYGGYVPNRPPLMR-- 816

Query: 747 MLTENHSDEILKDFRNYPENKLNELFPSVVQAAIVKQTMFLLSIHSPDEEYIGDAIEPA- 798
            L  + SD     F   P+       PS++Q       +  LS HSPDEEYIG+  +P+ 
Sbjct: 817 RLIPDESDPEFTSFIEDPQKYFFSSMPSLLQTTKFMAVVDTLSTHSPDEEYIGERQQPSI 876

BLAST of CSPI04G13500.1 vs. TAIR10
Match: AT1G55020.1 (AT1G55020.1 lipoxygenase 1)

HSP 1 Score: 434.9 bits (1117), Expect = 1.1e-121
Identity = 294/770 (38.18%), Postives = 399/770 (51.82%), Query Frame = 1

Query: 105 FGKIGAVIVELQDGVNERFVDTIYVEAEKPPISVTFSCKSWVQPKGLIDHQRIFFSSNKS 164
           FG  GA ++      +E  + ++ +E       V + C SW+ P       R+FFS NK+
Sbjct: 107 FGYPGAFLIR-NSHFSEFLLKSLTLEDVPGHGRVHYICNSWIYPAKHYTTDRVFFS-NKT 166

Query: 165 YLSGKTPGGLVKLREEDLANLRGEKADGSVDRNERKAFERIYDYDLYNDLGDPDQSMNLK 224
           YL  +TP  L+K REE+L +LRG          E K ++R+YDY  YNDLG P ++    
Sbjct: 167 YLPHETPATLLKYREEELVSLRG------TGEGELKEWDRVYDYAYYNDLGVPPKN---P 226

Query: 225 RPVLG--------------------DPASEKRIEEW----FYVPRDEEFSEIKQSSS--- 284
           RPVLG                    DP +E R+        YVPRDE F  +K S     
Sbjct: 227 RPVLGGTQEYPYPRRGRTGRKPTKEDPQTESRLPITSSLDIYVPRDERFGHLKMSDFLAY 286

Query: 285 ---------QPGNEKLLRKSPFS-DLPELEIKTPSAASKFNLQFNIASILSSHRPPALKS 344
                    QP  E +   +P   D  E  +K          Q  I SI+ +     LK 
Sbjct: 287 ALKAIAQFIQPALEAVFDDTPKEFDSFEDVLKIYEEGIDLPNQALIDSIVKNIPLEMLKE 346

Query: 345 S-DTPSSTLVELPPPESYKRDKYNWLSDIEFARLTLAGLNPYSIQLVKALADK-RLFVVD 404
              T     ++ P P+  K DK  W +D EFAR  LAGLNP  IQL+K    K +L    
Sbjct: 347 IFRTDGQKFLKFPVPQVIKEDKTAWRTDEEFAREMLAGLNPVVIQLLKEFPPKSKLDSES 406

Query: 405 YHD-----------------TLMPYVSKVR---------------KIKGTT--LYGSRTL 464
           Y +                 T+   + K R               ++  TT   Y SRTL
Sbjct: 407 YGNQNSTITKSHIEHNLDGLTVEEALEKERLFILDHHDTLMPYLGRVNTTTTKTYASRTL 466

Query: 465 FFLNSDGTLMPLGIELTRP-PMDGHLEQWKEVFTPGTNSTDVWLWRLAKAHVLSHDSCIH 524
            FL  DGTL PL IEL+ P P         EV+TPG    D  LW+LAKA V  +DS  H
Sbjct: 467 LFLKDDGTLKPLVIELSLPHPNGDKFGAVSEVYTPGEGVYDS-LWQLAKAFVGVNDSGNH 526

Query: 525 QLVIHWLRAHCCMEPYAIATNRQLSTMHPIYRLLHPHFRYNMRINANARKNLINAGGIIE 584
           QL+ HW++ H  +EP+ IATNRQLS +HP+++LL PHFR  M INA AR+ LIN GGI E
Sbjct: 527 QLISHWMQTHASIEPFVIATNRQLSVLHPVFKLLEPHFRDTMNINALARQILINGGGIFE 586

Query: 585 NAFSGASYSVELSSSAYK-AWRFDEQALPEDLIHRGMAERKRDSEGRDVLELTIKDYPFA 644
                + Y++E+SS  YK  W F +QALP +L  RGMA    D E    L L IKDYP+A
Sbjct: 587 ITVFPSKYAMEMSSFIYKNHWTFPDQALPAELKKRGMA--VEDPEAPHGLRLRIKDYPYA 646

Query: 645 NDGLLLWNALLEWVTEYVNHYYGDEENAVTNDKELQAWWGEIQAKGHPDKRE--GWPALR 704
            DGL +W A+  WV +Y+  +Y  EE+ +  D ELQAWW E++ +GH DK+    WP ++
Sbjct: 647 VDGLEVWYAIESWVRDYIFLFYKIEED-IQTDTELQAWWKEVREEGHGDKKSEPWWPKMQ 706

Query: 705 TRDDLIKIASTIAWVGSGHHASVNFLQYAYGGYMPNRPTIARTNMLTENHSDEILKDFRN 764
           TR++L++  + I WV S  HA+VNF QY   GY+PNRPTI+R  M  EN  +   ++   
Sbjct: 707 TREELVESCTIIIWVASALHAAVNFGQYPVAGYLPNRPTISRQYMPKENTPE--FEELEK 766

Query: 765 YPENKLNELFPSVVQAAIVKQTMFLLSIHSPDEEYIGDAIEPAWALDHSISKAFEKFKAK 798
            P+    +   + +Q  +    + +LS HS DE Y+G      WA +    +AFEKF  K
Sbjct: 767 NPDKVFLKTITAQLQTLLGISLIEILSTHSSDEVYLGQRDSKEWAAEKEALEAFEKFGEK 826

BLAST of CSPI04G13500.1 vs. NCBI nr
Match: gi|778693100|ref|XP_011653578.1| (PREDICTED: LOW QUALITY PROTEIN: lipoxygenase 2, chloroplastic-like [Cucumis sativus])

HSP 1 Score: 1473.4 bits (3813), Expect = 0.0e+00
Identity = 743/821 (90.50%), Postives = 747/821 (90.99%), Query Frame = 1

Query: 34  MSYMGTVVVTVIPKKNEQFPWVEIIYLKFASVELDSNEQPKEFIKCEAQLQEVDESVEYK 93
           MSYMGTVVVTVIPKKNEQFPWVEIIYLKFASVELDSNEQPKEFIKCEAQLQEVDESVEYK
Sbjct: 1   MSYMGTVVVTVIPKKNEQFPWVEIIYLKFASVELDSNEQPKEFIKCEAQLQEVDESVEYK 60

Query: 94  KYKGDIKVAEGFGKIGAVIVELQDGVNERFVDTIYVEAEKPPISVTFSCKSWVQPKGLID 153
           KYKGDIKVAEGFGKIGAVIVELQDGVNERFVDTI VEAEKPPISVTFSCKSWVQPKGLID
Sbjct: 61  KYKGDIKVAEGFGKIGAVIVELQDGVNERFVDTISVEAEKPPISVTFSCKSWVQPKGLID 120

Query: 154 HQRIFFSSNKSYLSGKTPGGLVKLREEDLANLRGEKADGSVDRNERKAFERIYDYDLYND 213
           H+RIFFSSNKSYLSGKTPGGLVKLREEDLANLRGEKADGSVDRNERKAFERIYDYDLYND
Sbjct: 121 HRRIFFSSNKSYLSGKTPGGLVKLREEDLANLRGEKADGSVDRNERKAFERIYDYDLYND 180

Query: 214 LGDPDQSMNLKRPVLG--------------------DPASEKRIEEWFYVPRDEEFSEIK 273
           LGDPDQSMNLKRPVLG                    DPASEKRIEEWFYVPRDEEFSEIK
Sbjct: 181 LGDPDQSMNLKRPVLGGSEEYPYPRRCRTGRPLTQNDPASEKRIEEWFYVPRDEEFSEIK 240

Query: 274 QSSSQPGNEKLLRKSPFSDLPELEIKTPSAASKFNLQFNIASILSSHRPPALKSSDTPSS 333
           QSSSQPGNEKLLRKSPFSDLPELEIKTPSAASKFNLQFNIASILSSHRPPALKSSDTPSS
Sbjct: 241 QSSSQPGNEKLLRKSPFSDLPELEIKTPSAASKFNLQFNIASILSSHRPPALKSSDTPSS 300

Query: 334 TLVELPPPESYKRDKYNWLSDIEF----------------------ARLTLAGLNPYS-- 393
           TLVELPPPESYKRDKYNWLSDIEF                      ++L      P    
Sbjct: 301 TLVELPPPESYKRDKYNWLSDIEFARLTLVGLNPYSIQLVKSLPFMSKLDEGDYRPRESK 360

Query: 394 -------------IQLVKALADKRLFVVDYHDTLMPYVSKVRKIKGTTLYGSRTLFFLNS 453
                        I + KALADKRLFVVDYHDTLMPYVSKVRKIKGTTLYGSRTLFFLNS
Sbjct: 361 FTPEXVQELLGCYITVDKALADKRLFVVDYHDTLMPYVSKVRKIKGTTLYGSRTLFFLNS 420

Query: 454 DGTLMPLGIELTRPPMDGHLEQWKEVFTPGTNSTDVWLWRLAKAHVLSHDSCIHQLVIHW 513
           DG LMPLGIELTRPPMDGHLEQWKEVFTPGTNSTDVWLWRLAKAHVLSHDSCIHQLVIHW
Sbjct: 421 DGILMPLGIELTRPPMDGHLEQWKEVFTPGTNSTDVWLWRLAKAHVLSHDSCIHQLVIHW 480

Query: 514 LRAHCCMEPYAIATNRQLSTMHPIYRLLHPHFRYNMRINANARKNLINAGGIIENAFSGA 573
           LRAHCCME YAIATNRQLSTMHPIYRLLHPHFRYNMRINANARKNLINA GIIENAFSGA
Sbjct: 481 LRAHCCMESYAIATNRQLSTMHPIYRLLHPHFRYNMRINANARKNLINARGIIENAFSGA 540

Query: 574 SYSVELSSSAYKAWRFDEQALPEDLIHRGMAERKRDSEGRDVLELTIKDYPFANDGLLLW 633
           SYSVELSSSAYKAWRFDEQALPEDLIHRGMAERKRDSEGRDVLELTIKDYPFANDGLLLW
Sbjct: 541 SYSVELSSSAYKAWRFDEQALPEDLIHRGMAERKRDSEGRDVLELTIKDYPFANDGLLLW 600

Query: 634 NALLEWVTEYVNHYYGDEENAVTNDKELQAWWGEIQAKGHPDKREGWPALRTRDDLIKIA 693
           NALLEWVTEYVNHYYGDEENAVTNDKELQAWWGEIQ KGHPDKREGWPALRTRDDLIKIA
Sbjct: 601 NALLEWVTEYVNHYYGDEENAVTNDKELQAWWGEIQEKGHPDKREGWPALRTRDDLIKIA 660

Query: 694 STIAWVGSGHHASVNFLQYAYGGYMPNRPTIARTNMLTENHSDEILKDFRNYPENKLNEL 753
           STIAWVGSGHHASVNFLQYAYGGYMPNRPTIARTNMLTENHSDEILKDFRNYPENKLNEL
Sbjct: 661 STIAWVGSGHHASVNFLQYAYGGYMPNRPTIARTNMLTENHSDEILKDFRNYPENKLNEL 720

Query: 754 FPSVVQAAIVKQTMFLLSIHSPDEEYIGDAIEPAWALDHSISKAFEKFKAKLTDLEKKID 798
           FPSVVQAAIVKQTMFLLSIHSPDEEYIGDAIEPAWALD SISKAFEKFKA LTDLEKKID
Sbjct: 721 FPSVVQAAIVKQTMFLLSIHSPDEEYIGDAIEPAWALDQSISKAFEKFKANLTDLEKKID 780

BLAST of CSPI04G13500.1 vs. NCBI nr
Match: gi|778693093|ref|XP_004142238.2| (PREDICTED: lipoxygenase 2, chloroplastic-like [Cucumis sativus])

HSP 1 Score: 1352.4 bits (3499), Expect = 0.0e+00
Identity = 684/823 (83.11%), Postives = 715/823 (86.88%), Query Frame = 1

Query: 34  MSYMGTVVVTVIPKKNEQFPWVEIIYLKFASVELDSNEQPKEFIKCEAQLQEVDESVEYK 93
           MSY GTVVVT+ PKK+EQF WVEIIYLKFASVELDSNEQPKEFIKCEAQLQEVDE VEYK
Sbjct: 1   MSYTGTVVVTMKPKKDEQFRWVEIIYLKFASVELDSNEQPKEFIKCEAQLQEVDELVEYK 60

Query: 94  KYKGDIKVAEGFGKIGAVIVELQDGVNERFVDTIYVEAEKPPISVTFSCKSWVQPKGLID 153
           KY G+I VAEGFG+IGAVIVELQ+GVNERF+DTI VEAE+PPIS+TFSCKSWVQPKGLID
Sbjct: 61  KYIGEIVVAEGFGEIGAVIVELQEGVNERFIDTISVEAEEPPISITFSCKSWVQPKGLID 120

Query: 154 HQRIFFSSNKSYLSGKTPGGLVKLREEDLANLRGEKADGSVDRNERKAFERIYDYDLYND 213
           H+RIFFSSNKSYL GKTP GLVKLREEDLANLRGEK DGSVDRNERKAFERIYDYDLYND
Sbjct: 121 HRRIFFSSNKSYLLGKTPRGLVKLREEDLANLRGEKEDGSVDRNERKAFERIYDYDLYND 180

Query: 214 LGDPDQSMNLKRPVLG--------------------DPASEKRIEEWFYVPRDEEFSEIK 273
           LGDPD SM+LKRPVLG                    DPASEKR+EEWFY+PRDEEFSEIK
Sbjct: 181 LGDPDLSMDLKRPVLGGSEEYPYPRRCRTGRPPTQNDPASEKRVEEWFYIPRDEEFSEIK 240

Query: 274 QSSSQPGNEKLLRKSPFSDLPELEIKTPSAASKFNLQFNIASILSSHRPP--ALKSSDTP 333
           QSS+QP N+KLL+K+P SDLPE+ IK PSAASK NLQ N++SI+ SH+PP  A+ SSD P
Sbjct: 241 QSSNQPRNKKLLKKAPSSDLPEIGIKMPSAASKINLQCNVSSIVCSHQPPAAAINSSDVP 300

Query: 334 SSTLVELPPPESYKRDKYNWLSDIEFARLTLAGLNPYSIQLVKALA-DKRLFVVDY---- 393
           SSTLVELPPPESYKRD+YNWLSDIEFARLTLAGLNPYSIQLVK+L    +L   DY    
Sbjct: 301 SSTLVELPPPESYKRDQYNWLSDIEFARLTLAGLNPYSIQLVKSLPFMSKLDEGDYGPRE 360

Query: 394 --------HDTL------------------------MPYVSKVRKIKGTTLYGSRTLFFL 453
                    + L                        MPYV +VRKIKGTTLYGSRTLFFL
Sbjct: 361 SKFTPERVQELLGCCITVGKALADKRLFVVDYHDTLMPYVRRVRKIKGTTLYGSRTLFFL 420

Query: 454 NSDGTLMPLGIELTRPPMDGHLEQWKEVFTPGTNSTDVWLWRLAKAHVLSHDSCIHQLVI 513
           NSDGTLMPLGIELTRPPMD HLEQWKEVFTPGTNSTD+WLWR AKAHVLSHDSCIHQLVI
Sbjct: 421 NSDGTLMPLGIELTRPPMDEHLEQWKEVFTPGTNSTDLWLWRFAKAHVLSHDSCIHQLVI 480

Query: 514 HWLRAHCCMEPYAIATNRQLSTMHPIYRLLHPHFRYNMRINANARKNLINAGGIIENAFS 573
           HWLRAHCCMEPYAIATNRQLSTMHPIYRLLHPHFRYNMRINANARKNLINAGGIIENAFS
Sbjct: 481 HWLRAHCCMEPYAIATNRQLSTMHPIYRLLHPHFRYNMRINANARKNLINAGGIIENAFS 540

Query: 574 GASYSVELSSSAYKAWRFDEQALPEDLIHRGMAERKRDSEGRDVLELTIKDYPFANDGLL 633
            ASYSVELSSSAYK WRFD QALPEDLIHRGMAERKRDSEGRDVLELTIKDYPFANDGLL
Sbjct: 541 AASYSVELSSSAYKEWRFDMQALPEDLIHRGMAERKRDSEGRDVLELTIKDYPFANDGLL 600

Query: 634 LWNALLEWVTEYVNHYYGDEENAVTNDKELQAWWGEIQAKGHPDKREGWPALRTRDDLIK 693
           LWNALLEWVTEYVNHYYGDEENAV ND ELQAWWGEIQ KGHPDKREGW ALRTRDDLIK
Sbjct: 601 LWNALLEWVTEYVNHYYGDEENAVINDTELQAWWGEIQEKGHPDKREGWFALRTRDDLIK 660

Query: 694 IASTIAWVGSGHHASVNFLQYAYGGYMPNRPTIARTNMLTENHSDEILKDFRNYPENKLN 753
           IASTIAWVGSGHHASVNFLQYAYGGYMPNRPTIARTNMLTENHSD+ LKDF N PE KLN
Sbjct: 661 IASTIAWVGSGHHASVNFLQYAYGGYMPNRPTIARTNMLTENHSDKFLKDFINQPEKKLN 720

Query: 754 ELFPSVVQAAIVKQTMFLLSIHSPDEEYIGDAIEPAWALDHSISKAFEKFKAKLTDLEKK 798
           ELFPS  QAAIVKQTMFLLSIHSPDEEYIGDAIEPAWALD SISKAFEKFKA LTDLEKK
Sbjct: 721 ELFPSDAQAAIVKQTMFLLSIHSPDEEYIGDAIEPAWALDQSISKAFEKFKANLTDLEKK 780

BLAST of CSPI04G13500.1 vs. NCBI nr
Match: gi|449448968|ref|XP_004142237.1| (PREDICTED: lipoxygenase 2, chloroplastic-like [Cucumis sativus])

HSP 1 Score: 1021.9 bits (2641), Expect = 5.9e-295
Identity = 533/826 (64.53%), Postives = 627/826 (75.91%), Query Frame = 1

Query: 42  VTVIPK-KNEQFPWVEIIYLKFASVELDSNEQPKEFIKCEAQ-LQEVDESVEYKKYKGDI 101
           VTV PK K   +P+  II LKFAS+ELDS++Q K FIKC  + +   DE+ EYKKY G++
Sbjct: 9   VTVKPKTKGPTYPYANII-LKFASIELDSDQQQKPFIKCVVEPILPDDETEEYKKYVGEV 68

Query: 102 KVAEGFGKIGAVIVELQDGVN--ERFVDTIYVEAEKPPISVTFSCKSWVQPKGLIDHQRI 161
           +V EG+G+IGAVIVEL+      E+F+DTI +  +K   S TFSCKSWVQ K ++D +R+
Sbjct: 69  EVPEGYGEIGAVIVELEGDTTKYEKFIDTISITDKKSRNSTTFSCKSWVQSKSVLDQRRV 128

Query: 162 FFSSNKSYLSGKTPGGLVKLREEDLANLRGEKADGSVDRNERKAFERIYDYDLYNDLGDP 221
           FFS+ KSYL  +TPGGL+KLR EDL+NLRG KAD +VD NERKAFERIYDYD YNDLGD 
Sbjct: 129 FFST-KSYLPERTPGGLLKLRAEDLSNLRGIKADETVDMNERKAFERIYDYDFYNDLGDC 188

Query: 222 DQSMNLKRPVLG-------------------DPASEKRIEEWFYVPRDEEFSEIKQ---S 281
           D     KRPVLG                   DP SEKR +E FYVPRDEEFSE+KQ    
Sbjct: 189 DGPAEWKRPVLGGSDHPYPRRCRTGRLHCKTDPVSEKRSKERFYVPRDEEFSEVKQHYFP 248

Query: 282 SSQPGNEKLLRKSPFSDLPELE------IKTPSAASKFNLQFNIASILSSHRPPALKSSD 341
           SS+P N+ LL K  FSDLP +E      IK P A  K  L FN+++I++  +P    +S+
Sbjct: 249 SSEPDNKDLLGKDSFSDLPHIESMFREGIKAPHAPHKL-LTFNLSTIVTPDKPDLHSNSN 308

Query: 342 TP-SSTLVELPPPESYKRDKYNWLSDIEFARLTLAGLNPYSIQLVKALADKRLFVVD--- 401
           +   S+L+  PPPESY+RD+Y+WLSD EFAR TLAGLNPYSIQLV  L        D   
Sbjct: 309 SSVPSSLLHYPPPESYRRDRYSWLSDTEFARQTLAGLNPYSIQLVTRLPLMSELDPDTYG 368

Query: 402 ----------------------------------YHDTLMPYVSKVRKIKGTTLYGSRTL 461
                                             YHDTLMPYV+KVRKI GTTLYGSRTL
Sbjct: 369 PQESAFNHTKVQELIGCSIEVNEAIELKRLFVLDYHDTLMPYVNKVRKINGTTLYGSRTL 428

Query: 462 FFLNSDGTLMPLGIELTRPPMDGHLEQWKEVFTPGTNSTDVWLWRLAKAHVLSHDSCIHQ 521
           FFL SD TL+PLGIELTRPP+DG+  QWK++FTPG  +TD+WLWR+AKAHVL+HDSCIHQ
Sbjct: 429 FFLKSDDTLIPLGIELTRPPVDGY-PQWKQIFTPGIEATDLWLWRIAKAHVLAHDSCIHQ 488

Query: 522 LVIHWLRAHCCMEPYAIATNRQLSTMHPIYRLLHPHFRYNMRINANARKNLINAGGIIEN 581
           LVIHWLRAHCCMEPYAIATNRQLST+HPIYRLLHPHFRYNMRINANAR++LINAGGIIE+
Sbjct: 489 LVIHWLRAHCCMEPYAIATNRQLSTVHPIYRLLHPHFRYNMRINANARESLINAGGIIES 548

Query: 582 AFSGASYSVELSSSAYK-AWRFDEQALPEDLIHRGMAERKRDSEGRDVLELTIKDYPFAN 641
            FS ASYSVELSSS Y+  WRFDEQALPEDLI RGMAERK+   GRDVLEL IKDYPFAN
Sbjct: 549 TFSTASYSVELSSSMYRDQWRFDEQALPEDLIRRGMAERKKGDYGRDVLELAIKDYPFAN 608

Query: 642 DGLLLWNALLEWVTEYVNHYYGDEENAVTNDKELQAWWGEIQAKGHPDKREGWPALRTRD 701
           DGLLLWNALLEWVTEYVNHYYGD+ENAV NDKELQAWW EIQ KGHPDK+EGWP L+TR+
Sbjct: 609 DGLLLWNALLEWVTEYVNHYYGDDENAVINDKELQAWWNEIQEKGHPDKKEGWPTLKTRN 668

Query: 702 DLIKIASTIAWVGSGHHASVNFLQYAYGGYMPNRPTIARTNMLTENHSDEILKDFRNYPE 761
           DLIKIASTIAWVGSGHHASVNF+QYAY GY PNRP+IARTN+LTE++  ++ ++F + PE
Sbjct: 669 DLIKIASTIAWVGSGHHASVNFIQYAYAGYTPNRPSIARTNILTEDY-HQLPEEFIDLPE 728

Query: 762 NKLNELFPSVVQAAIVKQTMFLLSIHSPDEEYIGDAIEPAWALDHSISKAFEKFKAKLTD 797
           N+L ++FPSV QA++V  TM LLS HSPDEEYIGD IEPAWAL+ SISKAF++F+A L D
Sbjct: 729 NELLQVFPSVDQASVVTTTMILLSAHSPDEEYIGDEIEPAWALEPSISKAFKRFQASLKD 788

BLAST of CSPI04G13500.1 vs. NCBI nr
Match: gi|659097677|ref|XP_008449754.1| (PREDICTED: lipoxygenase 2, chloroplastic-like [Cucumis melo])

HSP 1 Score: 1016.1 bits (2626), Expect = 3.2e-293
Identity = 529/833 (63.51%), Postives = 632/833 (75.87%), Query Frame = 1

Query: 34  MSYMGTVVVTVIPKKN-EQFPWVEIIYLKFASVELDSNEQPKEFIKCEAQ-LQEVDESVE 93
           M    TV VTV PK     +P+  II LKFAS+ELDS++Q K+FIKC  + ++  D++ E
Sbjct: 1   MGVEATVSVTVKPKTTGPTYPYANII-LKFASIELDSDQQQKQFIKCVVEPIRPDDDTEE 60

Query: 94  YKKYKGDIKVAEGFGKIGAVIVELQ--DGVNERFVDTIYVEAEKPPISVTFSCKSWVQPK 153
           YK+Y+G+++V EG+G+IGAVIVEL+      E+F+DTI +  +K   S TFSCKSWVQ K
Sbjct: 61  YKEYEGEVEVPEGYGEIGAVIVELECDTAKYEKFIDTISLTDKKSRHSTTFSCKSWVQSK 120

Query: 154 GLIDHQRIFFSSNKSYLSGKTPGGLVKLREEDLANLRGEKADGSVDRNERKAFERIYDYD 213
            ++D +RIFFS+ KSYL GKTPGGL+KLR EDL+NLRG K DG+VD NERKAFERIYDYD
Sbjct: 121 SVLDQRRIFFST-KSYLPGKTPGGLLKLRAEDLSNLRGIKPDGTVDMNERKAFERIYDYD 180

Query: 214 LYNDLGDPDQSMNLKRPVLG-------------------DPASEKRIEEWFYVPRDEEFS 273
            YNDLGD D     KRPVLG                   DP SEKR +E FYVPRDEEFS
Sbjct: 181 FYNDLGDCDGPAEWKRPVLGGSERPYPRRCRTGRLHCIADPVSEKRSKERFYVPRDEEFS 240

Query: 274 EIKQS---SSQPGNEKLLRKSPFSDLPELE------IKTPSAASKFNLQFNIASILSSHR 333
           E+KQ    SS+P N+ LL K+ F DLP +E      IK P A  K  L+FN+++I++   
Sbjct: 241 EVKQHYFPSSEPDNKDLLGKNSFHDLPHIESMFREGIKAPDAPHKL-LKFNLSTIVTPDH 300

Query: 334 PPALKSSDTPSSTLVELPPPESYKRDKYNWLSD---------------IEFA-RLTL--- 393
            P L S+ +  S+L+  PPPESY+RD+Y+WLSD               I+   RL L   
Sbjct: 301 KPDLHSNSSVPSSLLHYPPPESYRRDRYSWLSDTEFARQTLAGLNPYSIQLVTRLPLMSE 360

Query: 394 ----------AGLNPYSIQLV--------KALADKRLFVVDYHDTLMPYVSKVRKIKGTT 453
                     +  N   +Q +        +A+  KRLFVVDYHDTLMPYV+KVR I GTT
Sbjct: 361 LDPEIYGPQESAFNHTKVQELLGCFNEVNEAIECKRLFVVDYHDTLMPYVNKVRTINGTT 420

Query: 454 LYGSRTLFFLNSDGTLMPLGIELTRPPMDGHLEQWKEVFTPGTNSTDVWLWRLAKAHVLS 513
           LYGSRTLFFL SDGTL+PLGIELTRPP+DG+  QWK++FTPGT +TDVWLWR+AKAHVL+
Sbjct: 421 LYGSRTLFFLKSDGTLIPLGIELTRPPVDGY-PQWKQIFTPGTEATDVWLWRIAKAHVLA 480

Query: 514 HDSCIHQLVIHWLRAHCCMEPYAIATNRQLSTMHPIYRLLHPHFRYNMRINANARKNLIN 573
           HDSCIHQLVIHWLRAHCCMEPYAIATNRQLST+HPIYRLLHPHFRYNMRINANAR++LIN
Sbjct: 481 HDSCIHQLVIHWLRAHCCMEPYAIATNRQLSTLHPIYRLLHPHFRYNMRINANARESLIN 540

Query: 574 AGGIIENAFSGASYSVELSSSAYK-AWRFDEQALPEDLIHRGMAERKRDSEGRDVLELTI 633
           AGGIIE  FS ASYS+ELSSS Y+  WRFDEQA PEDLI RGMAERK+D  GRD+LELTI
Sbjct: 541 AGGIIEGTFSTASYSMELSSSVYRDQWRFDEQAFPEDLIRRGMAERKKDEYGRDILELTI 600

Query: 634 KDYPFANDGLLLWNALLEWVTEYVNHYYGDEENAVTNDKELQAWWGEIQAKGHPDKREGW 693
           KDYPFANDGL+LWNALLEWVTEYVNHYYGD+ENAV NDKELQAWW EIQ KGHPDK+EGW
Sbjct: 601 KDYPFANDGLILWNALLEWVTEYVNHYYGDDENAVINDKELQAWWNEIQEKGHPDKKEGW 660

Query: 694 PALRTRDDLIKIASTIAWVGSGHHASVNFLQYAYGGYMPNRPTIARTNMLTENHSDEILK 753
           P L+TR+DLIKIASTIAWVGSGHHASVNF+QYAY GY PNRP+IARTN+LTE++  ++ +
Sbjct: 661 PTLKTRNDLIKIASTIAWVGSGHHASVNFIQYAYAGYTPNRPSIARTNILTEDY-HQLPE 720

Query: 754 DFRNYPENKLNELFPSVVQAAIVKQTMFLLSIHSPDEEYIGDAIEPAWALDHSISKAFEK 797
           +F + PEN L ++FPSV QA +V  TM LLS HSPDEEYIG  +EPAWAL+ +I KAF++
Sbjct: 721 EFIDLPENVLLQVFPSVDQATMVTTTMILLSAHSPDEEYIGVEVEPAWALEPAIDKAFKR 780

BLAST of CSPI04G13500.1 vs. NCBI nr
Match: gi|778697632|ref|XP_011654363.1| (PREDICTED: LOW QUALITY PROTEIN: lipoxygenase 2, chloroplastic-like [Cucumis sativus])

HSP 1 Score: 1013.1 bits (2618), Expect = 2.7e-292
Identity = 528/767 (68.84%), Postives = 589/767 (76.79%), Query Frame = 1

Query: 93  KKYKGDIKVAEGFGKIGAVIVELQDGVNERFVDTIYVEAEKPPISVTFSCKSWVQPKGLI 152
           K ++  I V   FG+IGAVIVEL D V ERF+DTI ++ +K  + +TFSCKSWVQPK   
Sbjct: 26  KLFETSIDVPGDFGEIGAVIVELDDDVKERFIDTITIDFKKCTL-LTFSCKSWVQPKSST 85

Query: 153 DHQRIFFSSNKSYLSGKTPGGLVKLREEDLANLRGEKADGSVDRNERKAFERIYDYDLYN 212
             +RIFFSSNKSYLSGKTP GLVKLR EDLANLRGEK DGSVD+ +R AFERIYDYDLYN
Sbjct: 86  HQRRIFFSSNKSYLSGKTPEGLVKLRAEDLANLRGEKEDGSVDKKKRNAFERIYDYDLYN 145

Query: 213 DLGDPDQSMNLKRPVLG--------------------DPASEKRIEEWFYVPRDEEFSEI 272
           DLG PD+S +L RPVLG                    D  +E+RIE  FYVPRDE FSE 
Sbjct: 146 DLGKPDESSDLIRPVLGGSEEYPYPRRCRTGRDTSDNDKHAEERIEAPFYVPRDENFSEE 205

Query: 273 KQSSSQPGNEKLLRKSPFSDLPELEIKTPSAASKFN-LQFNIASILSSHRPPALKSSDTP 332
           KQ S    NE LL K+PFSDLPEL+IKTP AASK N L F+I S++SS++PP L S D  
Sbjct: 206 KQGSVLAENEALLGKAPFSDLPELDIKTPFAASKINQLPFDIPSLVSSNQPPPLNSLDDE 265

Query: 333 SSTLVELPPPESYK--RDKYNWLSD----------------------------------- 392
            S+ VELP PESYK   DKYNWLSD                                   
Sbjct: 266 PSSSVELPLPESYKISGDKYNWLSDIEFARLTLAGLNPYSIQLVKSLPFMSKLDEGDYGP 325

Query: 393 --IEFARLTLAGLNPYSIQLVKALADKRLFVVDYHDTLMPYVSKVRKIKGTTLYGSRTLF 452
              +F    +  L    I++ KAL DKRLFV+DYHD LMPYVSKVRKI+ TTLYGSRTLF
Sbjct: 326 RESKFTHELVQELLGCRIKVCKALTDKRLFVLDYHDILMPYVSKVRKIEWTTLYGSRTLF 385

Query: 453 FLNSDGTLMPLGIELTRPPMDGHLEQWKEVFTPGTNSTDVWLWRLAKAHVLSHDSCIHQL 512
           FLNSD TL PLGIELTRPP+ GH EQWKEVFTPGTNSTD+WLWR AKAHVLSHDSCIHQL
Sbjct: 386 FLNSDDTLEPLGIELTRPPIKGHSEQWKEVFTPGTNSTDLWLWRFAKAHVLSHDSCIHQL 445

Query: 513 VIHWLRAHCCMEPYAIATNRQLSTMHPIYRLLHPHFRYNMRINANARKNLINAGGIIENA 572
           VIHWLRAHCCMEPYAIAT+RQLSTMHPIYRLLHPHFRY MRINANARK LI AGGIIE  
Sbjct: 446 VIHWLRAHCCMEPYAIATHRQLSTMHPIYRLLHPHFRYTMRINANARKILICAGGIIEGT 505

Query: 573 FSGASYSVELSSSAYKAWRFDEQALPEDLIHRGMAERKRDSEGRDVLE-LTIKDYPFAND 632
           FS A +S+ELSSSAYK WRFD Q LP DL+HRGMAE K+D+E  +V++ LTIKDYPFAND
Sbjct: 506 FSAAYHSMELSSSAYKEWRFDLQGLPYDLVHRGMAETKKDAECHEVIDKLTIKDYPFAND 565

Query: 633 GLLLWNALLEWVTEYVNHYYGDEENAVTNDKELQAWWGEIQAKGHPDKREGWPALRTRDD 692
           GL LWNAL EWVTEYVNHYY  + NAVTNDKELQAWW EI+ KGHPD +EGWP L T++D
Sbjct: 566 GLQLWNALWEWVTEYVNHYYKCDMNAVTNDKELQAWWKEIKDKGHPDIKEGWPKLETKED 625

Query: 693 LIKIASTIAWVGSGHHASVNFLQYAYGGYMPNRPTIARTNMLTENH-SDEILKDFRNYPE 752
           LIKIASTIAWVGSGHHAS+NFLQYAY  Y+PNRP++AR NMLTEN+ S +I KDF N PE
Sbjct: 626 LIKIASTIAWVGSGHHASINFLQYAYXSYIPNRPSVARANMLTENNPSGKISKDFLNQPE 685

Query: 753 NKLNELFPSVVQAAIVKQTMFLLSIHSPDEEYIGDAIEPAWALDHSISKAFEKFKAKLTD 798
           +KL ELFP+  QA IV+QTMF+LSIHS DEEYIGD IEPAWAL+  ISKAFE+FKAKLTD
Sbjct: 686 DKLKELFPTDEQATIVQQTMFVLSIHSSDEEYIGDDIEPAWALEPCISKAFERFKAKLTD 745