CSPI04G12950.1 (mRNA) Wild cucumber (PI 183967)

NameCSPI04G12950.1
TypemRNA
OrganismCucumis sativus (Wild cucumber (PI 183967))
DescriptionUbiquitin-conjugating enzyme E2, putative
LocationChr4 : 11190900 .. 11194497 (+)
Sequence length558
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTCTAACACTACTGATGGATGATGGAATTGGGATGCAAAATCTGAACTATAGTGTTGAGAAATGAAAATCATGACCAGCAATTTGGTCTGATCACATACTTTGAACACTATCGTCATCATGTACAGCGATTACCGACTATGTTTATAATCAACTAATGGTCAGTGTTGGATTCAAAAATCTGAAATCTAATGGTGGGAAATCGAGACCACCGCCCGCCACTGCCTCCCTTAGGTGGGGGAAGATCGTCTTCTTCAATCTCCTTAGCTGCTCCAACTTCATGGGCGTCCACGACCTCGGTCTCTGCTTCAGGCAAGAGAATTCAGAGGGAAATGACTGAGTTTAACTTAGACCCACCTTCCGATTGCTCCGCTGGCCCTAAGGGCGACAATCTTTACCACTGGTTCGCTACCATCTTTGGCCCTTCAGGTAGTTGCGACTTTTCACTCTACTCTTTGGGCTTTATCGCTTCTTATTCGTACGGTTTTTGGCTTTTGTTCTTCTTTTTCTCCCTCCCTTTGCTCCATGATTTCTTGCTTTTCAGCTCGATGATTTCTTATGACCGTTAGGATTACACTTTCCTTCAGTGAGCTATTGCACTCTTTCTTTGTTATTTTTTTTTTTTATAGTTTTTGGGCTGGATAATTTAACCATCTAAAGCTATGTGGCTTATCTCATTTGTTTACGATTCCTGAACAGATGCTCACTGAAACTCTAATGGGGTTTTATTACCTGATTCAAAACATATTCTTTGAACAAGCAAATGTTTTACACAAGTTTAAAGAAAGTGATTAGGGAAGAAGATGTTCACTGTTGGGAGGAGTCAGGAGTGCATATACTGCTCCTTCAAGTACTTGGTGTGCTATGCTCCTCTGTCTCAGTGTATTTTGTGCAAATAATAAACGATTAGATTAGTTGCCAAGGAATTAAGACATAAGTTTTCACGCTGAATTAATGGCGTGAGAGTTGGAGAGAAACTACATTTAGACTAAAGTTGTTATCAAAAACAAATCTTTTATTAAAGTTGTTAGAGCAACAAGCTGAAATATCATCCAAAAATACACAAGGTTCTGCTATGGTTTTCCTACGTTGTTACACGGTCAATTCTCGTCTATATACTCAATATCACCCAAAAATATACAAAATGATTGGAGAAGATGCTCTCTGATACCAAAACTCTCAAGGATTTGGAAGAACGTTCTCTTGCTAAATAGCCACACGTCACTGTTGTACACACATATGCTTAAATGAATGAGATGAGTGCATGAAATCTGAGCCAGGATTTCAACTAAACACGATGAATACACAAGGAGAGTCATGTGTTGCTATAGGTCACTATTAAACTACCTTTCTCCTTTGCATCAAGTGAATTGTTTTATTAAAACAGTGTATTTTATTGGCTTCCTCATTGAGTCTTACTGAACGAATGGGTAGACAAGAGAGAGATTAACCTAGCTCATGCAATTCGTTTCTAGTATTAGTTTATTAGACAAGAAAAAAATGCACTGAAGGGCAAAAGAGGCATAGGTGATGTTTAATGGTATGGAAGGCGGACAATAGAGAGAAAAGCTGTGCTTATGCTGCTTATGCCTGATATTAGTGTTTTAGACAACAACAATACAAGAAAGCACCAATGGTGTATAAGTTATGTTTATCTACCATTTTGTTTAATGAAGTAAACTTCAGAAACCTTGTCCCAGCGAATCACTTCCTTTTCTTCTCTTTTCGTCCCAGGTTTTCTCTCTCTTGCGTACTAGACATGCACTACAAACCATTTGTCTATAAAGTTGTATTTATTATTGATTATATGAGGGCTTTGTTTGAATACTATCTTGAATATATGAGGAAAAATTACTGATAAAATTTATCTAGCTTTTCTCAACAGTAGTAATCTTAACAAATATCTCTGTCTTATTTTAGGAACGCCATATGAAGGTGGCATATTTTTCCTGGACATAATTTTTCCAAGTGATTATCCATTTAATCCTCCAAAGGTACTCTTGCTCACATCCTTAAATTTTAGGATACAAAAACCAAATTTTCTTTTACCTTCATCATGAGAATCAAATGGTCATTCTTGACCTGACACTGTACTGTTTTGAGTTTTATACTCATGTTTATTTACCAAGAAGGATTTGGTCTGCAGGTTGTATTTAAAACCCGAATCTACCACTGTAATGTTGATTCCACTGGTAATGTAAGTTTGGACATTTTGAAGGATAACTGGAGTCCTGCGTTAACAATCGCAAAGGTGTTAATTGCAGTGAAAACAATTTTCGCAAATCCCAACCCTTGTGAGTCGCCTCATTTTTTTGGACTCATTGTTTATGTCACTGTATACTGTAGTTTATAGTTCGTATGCTTTTCTATTTCTAGGGCTTCTCTAAAGCTAAACCTGCTCTCTTTTTATTTGGTAATTGTGAACTGAGACATAGGTGAACTGAAATATCGCACTGCATTGAGTTCTTTAAAATCAGTTACAAATTACAATTACAATCCATCTAAAGAGGCAGAGGGGAGTAGATACCTTTTATCAATATTAAGCACTAGGAGTGTAAGAATGAAAGGATGCAGATATATGCTTGGTTAGGTTGGGCTTTCAAAGGGAGCGATTTGGTGATTACTGGAAGGAATTTACTAGGAATAGAAACTAAATTGGTTGGGGGAACCAATGGAGGATTTGTAGAGAAATTGAATTAGTGTATATACAATTCTCCATGAATATATATGCTTGGCTATAGATAGTTACTGTATTTACTAGTAAAAAGAATTCTAGTTACAACGATTGCTGGTGGGATGACTATCTTGTGAAAAGGAATAGGCAGGGCTTTGTTATTGGTACCTAACCTGAATCCTGATCAAGGAGATGGTTGGTCCGTTTTCCATGAAAATATAGTGGGAATGAAGAGAAGGCATACATACAATGTCAATGTGCTATCTTTTCTACACAACTAATTGATCTAGTTGATGTTAACTTTGAAATCTCACATTCATTTTGGATTTACTAATTTTGCAGATGATCCAAATGCACCTGGTGTTGCTCACTTGTACTTGGCAGACAGAGCTAAACACGACGAGATAGCTGCTGAGTGGACGCTACGTTTCGCCAAATGAATGGAATCTCAAACCACTAATATACAATTGCATACTACTTTTTTGGTTATCATTTTCTGAAATTGCTTCCAATTCTCCCCCTTCACTTGGTAATTTACGTCTGTTTTCACAAGAAGTGACCAAGAACACCGGAAAGCAAATTTTGAGACGAGGACATGGAATAGAGTACAACCTGAAAGTTCTTGGATGCAGGAGTGGATAGTATAGATGATTTGTAGATTATTCTATATGTATCAATGTTCTTTTGGAGTGTTCAAGCCTTCAAGGTGCACTAGAACATATAGCCCTTCGGAATTTAGGTATCAATCAAAGGAAAAACGATTGTTATATGTATACATATATATATTATATATATATGATGCATATTTAATAGTCTTTTGGAGATTATTCTGATGATTCAGACAGATTTTAGCTGTACACATACACAAATAAATTTTGAACAGGAGTGTAAACACACAAATCATTAACATATTCTTTGAAACCAAG

mRNA sequence

ATGGTGGGAAATCGAGACCACCGCCCGCCACTGCCTCCCTTAGGTGGGGGAAGATCGTCTTCTTCAATCTCCTTAGCTGCTCCAACTTCATGGGCGTCCACGACCTCGGTCTCTGCTTCAGGCAAGAGAATTCAGAGGGAAATGACTGAGTTTAACTTAGACCCACCTTCCGATTGCTCCGCTGGCCCTAAGGGCGACAATCTTTACCACTGGTTCGCTACCATCTTTGGCCCTTCAGGAACGCCATATGAAGGTGGCATATTTTTCCTGGACATAATTTTTCCAAGTGATTATCCATTTAATCCTCCAAAGGTTGTATTTAAAACCCGAATCTACCACTGTAATGTTGATTCCACTGGTAATGTAAGTTTGGACATTTTGAAGGATAACTGGAGTCCTGCGTTAACAATCGCAAAGGTGTTAATTGCAGTGAAAACAATTTTCGCAAATCCCAACCCTTATGATCCAAATGCACCTGGTGTTGCTCACTTGTACTTGGCAGACAGAGCTAAACACGACGAGATAGCTGCTGAGTGGACGCTACGTTTCGCCAAATGA

Coding sequence (CDS)

ATGGTGGGAAATCGAGACCACCGCCCGCCACTGCCTCCCTTAGGTGGGGGAAGATCGTCTTCTTCAATCTCCTTAGCTGCTCCAACTTCATGGGCGTCCACGACCTCGGTCTCTGCTTCAGGCAAGAGAATTCAGAGGGAAATGACTGAGTTTAACTTAGACCCACCTTCCGATTGCTCCGCTGGCCCTAAGGGCGACAATCTTTACCACTGGTTCGCTACCATCTTTGGCCCTTCAGGAACGCCATATGAAGGTGGCATATTTTTCCTGGACATAATTTTTCCAAGTGATTATCCATTTAATCCTCCAAAGGTTGTATTTAAAACCCGAATCTACCACTGTAATGTTGATTCCACTGGTAATGTAAGTTTGGACATTTTGAAGGATAACTGGAGTCCTGCGTTAACAATCGCAAAGGTGTTAATTGCAGTGAAAACAATTTTCGCAAATCCCAACCCTTATGATCCAAATGCACCTGGTGTTGCTCACTTGTACTTGGCAGACAGAGCTAAACACGACGAGATAGCTGCTGAGTGGACGCTACGTTTCGCCAAATGA
BLAST of CSPI04G12950.1 vs. Swiss-Prot
Match: COP10_ARATH (Constitutive photomorphogenesis protein 10 OS=Arabidopsis thaliana GN=COP10 PE=1 SV=1)

HSP 1 Score: 265.8 bits (678), Expect = 3.6e-70
Identity = 119/157 (75.80%), Postives = 135/157 (85.99%), Query Frame = 1

Query: 29  TSWASTTSVSASGKRIQREMTEFNLDPPSDCSAGPKGDNLYHWFATIFGPSGTPYEGGIF 88
           +SW + TSVSASGKRIQREM E N+DPP DCSAGPKGDNLYHW ATI GPSGTPYEGGIF
Sbjct: 26  SSWVAKTSVSASGKRIQREMAELNIDPPPDCSAGPKGDNLYHWIATIIGPSGTPYEGGIF 85

Query: 89  FLDIIFPSDYPFNPPKVVFKTRIYHCNVDSTGNVSLDILKDNWSPALTIAKVLIAVKTIF 148
           FLDIIFPSDYPF PPK+VFKTRIYHCNVD+ G++S++IL+D+WSPALTI KVL A+++IF
Sbjct: 86  FLDIIFPSDYPFKPPKLVFKTRIYHCNVDTAGDLSVNILRDSWSPALTITKVLQAIRSIF 145

Query: 149 ANPNPYDPNAPGVAHLYLADRAKHDEIAAEWTLRFAK 186
             P PY P  P +A LYL DR KHDE+A EWTLRFAK
Sbjct: 146 LKPEPYSPALPVIARLYLTDREKHDEVAKEWTLRFAK 182

BLAST of CSPI04G12950.1 vs. Swiss-Prot
Match: UB2E3_MOUSE (Ubiquitin-conjugating enzyme E2 E3 OS=Mus musculus GN=Ube2e3 PE=1 SV=2)

HSP 1 Score: 208.0 bits (528), Expect = 9.0e-53
Identity = 100/182 (54.95%), Postives = 127/182 (69.78%), Query Frame = 1

Query: 5   RDHRPPLPPLGGGRSSSSISLAAPTSWASTTS--VSASGKRIQREMTEFNLDPPSDCSAG 64
           RD   P P     R  S+      T  +S T+  +S S KRIQ+E+ E  LDPP +CSAG
Sbjct: 25  RDPAAPEPEEQEERKPSATQQKKNTKLSSKTTAKLSTSAKRIQKELAEITLDPPPNCSAG 84

Query: 65  PKGDNLYHWFATIFGPSGTPYEGGIFFLDIIFPSDYPFNPPKVVFKTRIYHCNVDSTGNV 124
           PKGDN+Y W +TI GP G+ YEGG+FFLDI F SDYPF PPKV F+TRIYHCN++S G +
Sbjct: 85  PKGDNIYEWRSTILGPPGSVYEGGVFFLDITFSSDYPFKPPKVTFRTRIYHCNINSQGVI 144

Query: 125 SLDILKDNWSPALTIAKVLIAVKTIFANPNPYDPNAPGVAHLYLADRAKHDEIAAEWTLR 184
            LDILKDNWSPALTI+KVL+++ ++  + NP DP    +A  YL +RA+HD IA +WT R
Sbjct: 145 CLDILKDNWSPALTISKVLLSICSLLTDCNPADPLVGSIATQYLTNRAEHDRIARQWTKR 204

BLAST of CSPI04G12950.1 vs. Swiss-Prot
Match: UB2E3_HUMAN (Ubiquitin-conjugating enzyme E2 E3 OS=Homo sapiens GN=UBE2E3 PE=1 SV=1)

HSP 1 Score: 208.0 bits (528), Expect = 9.0e-53
Identity = 100/182 (54.95%), Postives = 127/182 (69.78%), Query Frame = 1

Query: 5   RDHRPPLPPLGGGRSSSSISLAAPTSWASTTS--VSASGKRIQREMTEFNLDPPSDCSAG 64
           RD   P P     R  S+      T  +S T+  +S S KRIQ+E+ E  LDPP +CSAG
Sbjct: 25  RDPAAPEPEEQEERKPSATQQKKNTKLSSKTTAKLSTSAKRIQKELAEITLDPPPNCSAG 84

Query: 65  PKGDNLYHWFATIFGPSGTPYEGGIFFLDIIFPSDYPFNPPKVVFKTRIYHCNVDSTGNV 124
           PKGDN+Y W +TI GP G+ YEGG+FFLDI F SDYPF PPKV F+TRIYHCN++S G +
Sbjct: 85  PKGDNIYEWRSTILGPPGSVYEGGVFFLDITFSSDYPFKPPKVTFRTRIYHCNINSQGVI 144

Query: 125 SLDILKDNWSPALTIAKVLIAVKTIFANPNPYDPNAPGVAHLYLADRAKHDEIAAEWTLR 184
            LDILKDNWSPALTI+KVL+++ ++  + NP DP    +A  YL +RA+HD IA +WT R
Sbjct: 145 CLDILKDNWSPALTISKVLLSICSLLTDCNPADPLVGSIATQYLTNRAEHDRIARQWTKR 204

BLAST of CSPI04G12950.1 vs. Swiss-Prot
Match: UB2E3_BOVIN (Ubiquitin-conjugating enzyme E2 E3 OS=Bos taurus GN=UBE2E3 PE=2 SV=1)

HSP 1 Score: 208.0 bits (528), Expect = 9.0e-53
Identity = 100/182 (54.95%), Postives = 127/182 (69.78%), Query Frame = 1

Query: 5   RDHRPPLPPLGGGRSSSSISLAAPTSWASTTS--VSASGKRIQREMTEFNLDPPSDCSAG 64
           RD   P P     R  S+      T  +S T+  +S S KRIQ+E+ E  LDPP +CSAG
Sbjct: 25  RDPAAPEPEEQEERKPSATQQKKNTKLSSKTTAKLSTSAKRIQKELAEITLDPPPNCSAG 84

Query: 65  PKGDNLYHWFATIFGPSGTPYEGGIFFLDIIFPSDYPFNPPKVVFKTRIYHCNVDSTGNV 124
           PKGDN+Y W +TI GP G+ YEGG+FFLDI F SDYPF PPKV F+TRIYHCN++S G +
Sbjct: 85  PKGDNIYEWRSTILGPPGSVYEGGVFFLDITFSSDYPFKPPKVTFRTRIYHCNINSQGVI 144

Query: 125 SLDILKDNWSPALTIAKVLIAVKTIFANPNPYDPNAPGVAHLYLADRAKHDEIAAEWTLR 184
            LDILKDNWSPALTI+KVL+++ ++  + NP DP    +A  YL +RA+HD IA +WT R
Sbjct: 145 CLDILKDNWSPALTISKVLLSICSLLTDCNPADPLVGSIATQYLTNRAEHDRIARQWTKR 204

BLAST of CSPI04G12950.1 vs. Swiss-Prot
Match: UB2E2_MOUSE (Ubiquitin-conjugating enzyme E2 E2 OS=Mus musculus GN=Ube2e2 PE=2 SV=1)

HSP 1 Score: 203.4 bits (516), Expect = 2.2e-51
Identity = 88/152 (57.89%), Postives = 115/152 (75.66%), Query Frame = 1

Query: 33  STTSVSASGKRIQREMTEFNLDPPSDCSAGPKGDNLYHWFATIFGPSGTPYEGGIFFLDI 92
           +   +S S KRIQ+E+ E  LDPP +CSAGPKGDN+Y W +TI GP G+ YEGG+FFLDI
Sbjct: 49  TAAKLSTSAKRIQKELAEITLDPPPNCSAGPKGDNIYEWRSTILGPPGSVYEGGVFFLDI 108

Query: 93  IFPSDYPFNPPKVVFKTRIYHCNVDSTGNVSLDILKDNWSPALTIAKVLIAVKTIFANPN 152
            F  DYPF PPKV F+TRIYHCN++S G + LDILKDNWSPALTI+KVL+++ ++  + N
Sbjct: 109 TFSPDYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSICSLLTDCN 168

Query: 153 PYDPNAPGVAHLYLADRAKHDEIAAEWTLRFA 185
           P DP    +A  Y+ +RA+HD +A +WT R+A
Sbjct: 169 PADPLVGSIATQYMTNRAEHDRMARQWTKRYA 200

BLAST of CSPI04G12950.1 vs. TrEMBL
Match: A0A0A0KWU2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G280420 PE=4 SV=1)

HSP 1 Score: 386.7 bits (992), Expect = 1.6e-104
Identity = 184/185 (99.46%), Postives = 185/185 (100.00%), Query Frame = 1

Query: 1   MVGNRDHRPPLPPLGGGRSSSSISLAAPTSWASTTSVSASGKRIQREMTEFNLDPPSDCS 60
           MVGNRDHRPPLPPLGGGRSSSSISLAAPTSWASTTSVSASGKRIQREMTEFNLDPPSDCS
Sbjct: 1   MVGNRDHRPPLPPLGGGRSSSSISLAAPTSWASTTSVSASGKRIQREMTEFNLDPPSDCS 60

Query: 61  AGPKGDNLYHWFATIFGPSGTPYEGGIFFLDIIFPSDYPFNPPKVVFKTRIYHCNVDSTG 120
           AGPKGDNLYHWFATIFGPSGTPYEGGIFFLDIIFPSDYPFNPPKVVFKTRIYHCNVDSTG
Sbjct: 61  AGPKGDNLYHWFATIFGPSGTPYEGGIFFLDIIFPSDYPFNPPKVVFKTRIYHCNVDSTG 120

Query: 121 NVSLDILKDNWSPALTIAKVLIAVKTIFANPNPYDPNAPGVAHLYLADRAKHDEIAAEWT 180
           NVSLDILKDNWSPALTIAKVLIAVK+IFANPNPYDPNAPGVAHLYLADRAKHDEIAAEWT
Sbjct: 121 NVSLDILKDNWSPALTIAKVLIAVKSIFANPNPYDPNAPGVAHLYLADRAKHDEIAAEWT 180

Query: 181 LRFAK 186
           LRFAK
Sbjct: 181 LRFAK 185

BLAST of CSPI04G12950.1 vs. TrEMBL
Match: A0A061GS19_THECC (Ubiquitin-conjugating enzyme family protein isoform 1 OS=Theobroma cacao GN=TCM_040041 PE=4 SV=1)

HSP 1 Score: 285.8 bits (730), Expect = 3.8e-74
Identity = 133/174 (76.44%), Postives = 150/174 (86.21%), Query Frame = 1

Query: 14  LGGGRSSSS--ISLAAPTSWASTTSVSASGKRIQREMTEFNLDPPSDCSAGPKGDNLYHW 73
           +GGGRSSSS   + AA  +W STTSVS SGKRIQREM+E N+DPP  CSAGPKGDNLYHW
Sbjct: 3   IGGGRSSSSSTTATAAGGTWVSTTSVSTSGKRIQREMSELNVDPPPHCSAGPKGDNLYHW 62

Query: 74  FATIFGPSGTPYEGGIFFLDIIFPSDYPFNPPKVVFKTRIYHCNVDSTGNVSLDILKDNW 133
            ATI GP GTPY+GGIFFLDI FPSDYPF PPKVVFKTRIYHCNVD+ GN+SLDILKD+W
Sbjct: 63  VATIIGPPGTPYQGGIFFLDITFPSDYPFKPPKVVFKTRIYHCNVDAAGNLSLDILKDSW 122

Query: 134 SPALTIAKVLIAVKTIFANPNPYDPNAPGVAHLYLADRAKHDEIAAEWTLRFAK 186
           SPALT+ KVL A+K+IF NP+PY+P+  G+A LYL D+AKHDEIAAEWTLRFAK
Sbjct: 123 SPALTVTKVLTAIKSIFTNPDPYNPHVSGIARLYLTDKAKHDEIAAEWTLRFAK 176

BLAST of CSPI04G12950.1 vs. TrEMBL
Match: B9GL37_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0001s10830g PE=4 SV=2)

HSP 1 Score: 285.4 bits (729), Expect = 4.9e-74
Identity = 130/171 (76.02%), Postives = 150/171 (87.72%), Query Frame = 1

Query: 15  GGGRSSSSISLAAPTSWASTTSVSASGKRIQREMTEFNLDPPSDCSAGPKGDNLYHWFAT 74
           GGG SS++    A +SW S +SVS SGKRIQREMTE N+DPP DCSAGPKGDNLYHW +T
Sbjct: 11  GGGGSSAT----ATSSWVSPSSVSTSGKRIQREMTELNMDPPPDCSAGPKGDNLYHWIST 70

Query: 75  IFGPSGTPYEGGIFFLDIIFPSDYPFNPPKVVFKTRIYHCNVDSTGNVSLDILKDNWSPA 134
           + GP GTPY+GGIFFLD+ FPSDYPF PPKVVFKTRIYHCNVDS GN+SLDILKD+WSPA
Sbjct: 71  VIGPPGTPYQGGIFFLDLTFPSDYPFKPPKVVFKTRIYHCNVDSAGNLSLDILKDSWSPA 130

Query: 135 LTIAKVLIAVKTIFANPNPYDPNAPGVAHLYLADRAKHDEIAAEWTLRFAK 186
           LTI KVL+A+++IF +P+PY+P  PG+AHLYLADRAKHDE+AAEWTLRFAK
Sbjct: 131 LTITKVLLAIRSIFTHPDPYNPLVPGIAHLYLADRAKHDELAAEWTLRFAK 177

BLAST of CSPI04G12950.1 vs. TrEMBL
Match: B9SM61_RICCO (Ubiquitin-conjugating enzyme E2, putative OS=Ricinus communis GN=RCOM_0878930 PE=4 SV=1)

HSP 1 Score: 283.1 bits (723), Expect = 2.4e-73
Identity = 127/171 (74.27%), Postives = 147/171 (85.96%), Query Frame = 1

Query: 15  GGGRSSSSISLAAPTSWASTTSVSASGKRIQREMTEFNLDPPSDCSAGPKGDNLYHWFAT 74
           GGG  S++ + A  +SW S TSVS SGKRIQREM E N DPP DCSAGPKGDNLYHW +T
Sbjct: 11  GGGGGSAAAAAAPSSSWVSPTSVSTSGKRIQREMGELNNDPPPDCSAGPKGDNLYHWVST 70

Query: 75  IFGPSGTPYEGGIFFLDIIFPSDYPFNPPKVVFKTRIYHCNVDSTGNVSLDILKDNWSPA 134
           I GP GTPYEGGIFFLDI FP+DYPF PPKVVFKTRIYHCNVDS GN+SLD+LKD WSPA
Sbjct: 71  IIGPPGTPYEGGIFFLDITFPTDYPFKPPKVVFKTRIYHCNVDSAGNLSLDMLKDGWSPA 130

Query: 135 LTIAKVLIAVKTIFANPNPYDPNAPGVAHLYLADRAKHDEIAAEWTLRFAK 186
           LTI K+LIA++++F +P+PY+P  PG+AHLYLADRAKHDE+AA+WT+RFAK
Sbjct: 131 LTITKILIAIRSVFTHPDPYNPLVPGIAHLYLADRAKHDELAAQWTMRFAK 181

BLAST of CSPI04G12950.1 vs. TrEMBL
Match: A0A0D2R6Z2_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_010G126700 PE=4 SV=1)

HSP 1 Score: 281.2 bits (718), Expect = 9.3e-73
Identity = 133/174 (76.44%), Postives = 150/174 (86.21%), Query Frame = 1

Query: 14  LGGGRSSSSISLA--APTSWASTTSVSASGKRIQREMTEFNLDPPSDCSAGPKGDNLYHW 73
           +GGGRSSSS S A  A  +W STTSVSASGKRIQREM+E N DPP  CSA PKGDNL+HW
Sbjct: 2   IGGGRSSSSSSTATAAGGTWVSTTSVSASGKRIQREMSELNADPPPHCSAAPKGDNLFHW 61

Query: 74  FATIFGPSGTPYEGGIFFLDIIFPSDYPFNPPKVVFKTRIYHCNVDSTGNVSLDILKDNW 133
            ATI GP GTPY+GGIFFLDI FPSDYPF PPKVVFKTRIYHCNVD+ GN+SLDILKD+W
Sbjct: 62  VATIIGPPGTPYQGGIFFLDITFPSDYPFQPPKVVFKTRIYHCNVDAAGNLSLDILKDSW 121

Query: 134 SPALTIAKVLIAVKTIFANPNPYDPNAPGVAHLYLADRAKHDEIAAEWTLRFAK 186
           SPALTI+KVL A+++IF+NP+PY P   GVA LYLAD+AKHDEIA+EWT+RFAK
Sbjct: 122 SPALTISKVLTAIRSIFSNPDPYSPLTSGVARLYLADKAKHDEIASEWTVRFAK 175

BLAST of CSPI04G12950.1 vs. TAIR10
Match: AT3G13550.1 (AT3G13550.1 Ubiquitin-conjugating enzyme family protein)

HSP 1 Score: 265.8 bits (678), Expect = 2.0e-71
Identity = 119/157 (75.80%), Postives = 135/157 (85.99%), Query Frame = 1

Query: 29  TSWASTTSVSASGKRIQREMTEFNLDPPSDCSAGPKGDNLYHWFATIFGPSGTPYEGGIF 88
           +SW + TSVSASGKRIQREM E N+DPP DCSAGPKGDNLYHW ATI GPSGTPYEGGIF
Sbjct: 26  SSWVAKTSVSASGKRIQREMAELNIDPPPDCSAGPKGDNLYHWIATIIGPSGTPYEGGIF 85

Query: 89  FLDIIFPSDYPFNPPKVVFKTRIYHCNVDSTGNVSLDILKDNWSPALTIAKVLIAVKTIF 148
           FLDIIFPSDYPF PPK+VFKTRIYHCNVD+ G++S++IL+D+WSPALTI KVL A+++IF
Sbjct: 86  FLDIIFPSDYPFKPPKLVFKTRIYHCNVDTAGDLSVNILRDSWSPALTITKVLQAIRSIF 145

Query: 149 ANPNPYDPNAPGVAHLYLADRAKHDEIAAEWTLRFAK 186
             P PY P  P +A LYL DR KHDE+A EWTLRFAK
Sbjct: 146 LKPEPYSPALPVIARLYLTDREKHDEVAKEWTLRFAK 182

BLAST of CSPI04G12950.1 vs. TAIR10
Match: AT2G16740.1 (AT2G16740.1 ubiquitin-conjugating enzyme 29)

HSP 1 Score: 186.8 bits (473), Expect = 1.2e-47
Identity = 75/143 (52.45%), Postives = 109/143 (76.22%), Query Frame = 1

Query: 42  KRIQREMTEFNLDPPSDCSAGPKGDNLYHWFATIFGPSGTPYEGGIFFLDIIFPSDYPFN 101
           +RI +E+ E   DPP  CSAGP G++++HW ATI GP+ +PY GG+F ++I FP DYPF 
Sbjct: 4   RRILKELKELQRDPPVSCSAGPTGEDMFHWQATIMGPNESPYSGGVFLVNIHFPPDYPFK 63

Query: 102 PPKVVFKTRIYHCNVDSTGNVSLDILKDNWSPALTIAKVLIAVKTIFANPNPYDPNAPGV 161
           PPKVVF+T+++H N++S GN+ LDILKD WSPALTI+KVL+++ ++  +PNP DP  P +
Sbjct: 64  PPKVVFRTKVFHPNINSNGNICLDILKDQWSPALTISKVLLSICSLLTDPNPDDPLVPEI 123

Query: 162 AHLYLADRAKHDEIAAEWTLRFA 185
           AH+Y  D+ K++ +A  WT ++A
Sbjct: 124 AHIYKTDKTKYEAMARSWTQKYA 146

BLAST of CSPI04G12950.1 vs. TAIR10
Match: AT3G08690.1 (AT3G08690.1 ubiquitin-conjugating enzyme 11)

HSP 1 Score: 183.3 bits (464), Expect = 1.3e-46
Identity = 75/145 (51.72%), Postives = 108/145 (74.48%), Query Frame = 1

Query: 40  SGKRIQREMTEFNLDPPSDCSAGPKGDNLYHWFATIFGPSGTPYEGGIFFLDIIFPSDYP 99
           + KRI +E+ +   DPPS+CSAGP  ++++HW ATI GP  +PY GG+F + I FP DYP
Sbjct: 2   ASKRILKELKDLQKDPPSNCSAGPVAEDMFHWQATIMGPPESPYAGGVFLVSIHFPPDYP 61

Query: 100 FNPPKVVFKTRIYHCNVDSTGNVSLDILKDNWSPALTIAKVLIAVKTIFANPNPYDPNAP 159
           F PPKV FKT++YH N++S G++ LDILK+ WSPALTI+KVL+++ ++  +PNP DP  P
Sbjct: 62  FKPPKVSFKTKVYHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVP 121

Query: 160 GVAHLYLADRAKHDEIAAEWTLRFA 185
            +AH+Y  DR+K++  A  WT ++A
Sbjct: 122 EIAHMYKTDRSKYESTARSWTQKYA 146

BLAST of CSPI04G12950.1 vs. TAIR10
Match: AT5G56150.1 (AT5G56150.1 ubiquitin-conjugating enzyme 30)

HSP 1 Score: 180.6 bits (457), Expect = 8.6e-46
Identity = 72/145 (49.66%), Postives = 106/145 (73.10%), Query Frame = 1

Query: 40  SGKRIQREMTEFNLDPPSDCSAGPKGDNLYHWFATIFGPSGTPYEGGIFFLDIIFPSDYP 99
           + KRI +E+ +   DPP  CSAGP GD+++ W ATI GP+ +P+ GG+F + I FP DYP
Sbjct: 2   ASKRINKELRDLQRDPPVSCSAGPTGDDMFQWQATIMGPADSPFAGGVFLVTIHFPPDYP 61

Query: 100 FNPPKVVFKTRIYHCNVDSTGNVSLDILKDNWSPALTIAKVLIAVKTIFANPNPYDPNAP 159
           F PPKV F+T++YH N++S G++ LDILK+ WSPALT++KVL+++ ++  +PNP DP  P
Sbjct: 62  FKPPKVAFRTKVYHPNINSNGSICLDILKEQWSPALTVSKVLLSICSLLTDPNPDDPLVP 121

Query: 160 GVAHLYLADRAKHDEIAAEWTLRFA 185
            +AH+Y  DR K++  A  WT ++A
Sbjct: 122 EIAHIYKTDRVKYESTAQSWTQKYA 146

BLAST of CSPI04G12950.1 vs. TAIR10
Match: AT4G27960.2 (AT4G27960.2 ubiquitin conjugating enzyme 9)

HSP 1 Score: 179.5 bits (454), Expect = 1.9e-45
Identity = 72/145 (49.66%), Postives = 107/145 (73.79%), Query Frame = 1

Query: 40  SGKRIQREMTEFNLDPPSDCSAGPKGDNLYHWFATIFGPSGTPYEGGIFFLDIIFPSDYP 99
           + KRI +E+ +   DPP+ CSAGP  ++++HW ATI GPS +PY GG+F + I FP DYP
Sbjct: 32  ASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVTIHFPPDYP 91

Query: 100 FNPPKVVFKTRIYHCNVDSTGNVSLDILKDNWSPALTIAKVLIAVKTIFANPNPYDPNAP 159
           F PPKV F+T+++H N++S G++ LDILK+ WSPALTI+KVL+++ ++  +PNP DP  P
Sbjct: 92  FKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVP 151

Query: 160 GVAHLYLADRAKHDEIAAEWTLRFA 185
            +AH+Y  D+ K++  A  WT ++A
Sbjct: 152 EIAHMYKTDKNKYESTARTWTQKYA 176

BLAST of CSPI04G12950.1 vs. NCBI nr
Match: gi|449448836|ref|XP_004142171.1| (PREDICTED: constitutive photomorphogenesis protein 10 [Cucumis sativus])

HSP 1 Score: 386.7 bits (992), Expect = 2.2e-104
Identity = 184/185 (99.46%), Postives = 185/185 (100.00%), Query Frame = 1

Query: 1   MVGNRDHRPPLPPLGGGRSSSSISLAAPTSWASTTSVSASGKRIQREMTEFNLDPPSDCS 60
           MVGNRDHRPPLPPLGGGRSSSSISLAAPTSWASTTSVSASGKRIQREMTEFNLDPPSDCS
Sbjct: 1   MVGNRDHRPPLPPLGGGRSSSSISLAAPTSWASTTSVSASGKRIQREMTEFNLDPPSDCS 60

Query: 61  AGPKGDNLYHWFATIFGPSGTPYEGGIFFLDIIFPSDYPFNPPKVVFKTRIYHCNVDSTG 120
           AGPKGDNLYHWFATIFGPSGTPYEGGIFFLDIIFPSDYPFNPPKVVFKTRIYHCNVDSTG
Sbjct: 61  AGPKGDNLYHWFATIFGPSGTPYEGGIFFLDIIFPSDYPFNPPKVVFKTRIYHCNVDSTG 120

Query: 121 NVSLDILKDNWSPALTIAKVLIAVKTIFANPNPYDPNAPGVAHLYLADRAKHDEIAAEWT 180
           NVSLDILKDNWSPALTIAKVLIAVK+IFANPNPYDPNAPGVAHLYLADRAKHDEIAAEWT
Sbjct: 121 NVSLDILKDNWSPALTIAKVLIAVKSIFANPNPYDPNAPGVAHLYLADRAKHDEIAAEWT 180

Query: 181 LRFAK 186
           LRFAK
Sbjct: 181 LRFAK 185

BLAST of CSPI04G12950.1 vs. NCBI nr
Match: gi|659097798|ref|XP_008449820.1| (PREDICTED: constitutive photomorphogenesis protein 10 [Cucumis melo])

HSP 1 Score: 379.0 bits (972), Expect = 4.7e-102
Identity = 181/185 (97.84%), Postives = 182/185 (98.38%), Query Frame = 1

Query: 1   MVGNRDHRPPLPPLGGGRSSSSISLAAPTSWASTTSVSASGKRIQREMTEFNLDPPSDCS 60
           MVGNRD RP LPPLGGGRSSSSISLAAPTSWASTTSVSASGKRIQREMTEFNLDPPSDCS
Sbjct: 1   MVGNRDQRPQLPPLGGGRSSSSISLAAPTSWASTTSVSASGKRIQREMTEFNLDPPSDCS 60

Query: 61  AGPKGDNLYHWFATIFGPSGTPYEGGIFFLDIIFPSDYPFNPPKVVFKTRIYHCNVDSTG 120
           AGPKGDNLYHWFATIFGPSGTPYEGGIFFLDIIFPSDYPFNPPKVVFKTRIYHCNVDSTG
Sbjct: 61  AGPKGDNLYHWFATIFGPSGTPYEGGIFFLDIIFPSDYPFNPPKVVFKTRIYHCNVDSTG 120

Query: 121 NVSLDILKDNWSPALTIAKVLIAVKTIFANPNPYDPNAPGVAHLYLADRAKHDEIAAEWT 180
           NVSLDILKDNWSPALTIAKVLIAVK+IF NPNPYDPNAPGVAHLYLADRAKHDEIAAEWT
Sbjct: 121 NVSLDILKDNWSPALTIAKVLIAVKSIFVNPNPYDPNAPGVAHLYLADRAKHDEIAAEWT 180

Query: 181 LRFAK 186
           LRFAK
Sbjct: 181 LRFAK 185

BLAST of CSPI04G12950.1 vs. NCBI nr
Match: gi|590582669|ref|XP_007014687.1| (Ubiquitin-conjugating enzyme family protein isoform 1 [Theobroma cacao])

HSP 1 Score: 285.8 bits (730), Expect = 5.4e-74
Identity = 133/174 (76.44%), Postives = 150/174 (86.21%), Query Frame = 1

Query: 14  LGGGRSSSS--ISLAAPTSWASTTSVSASGKRIQREMTEFNLDPPSDCSAGPKGDNLYHW 73
           +GGGRSSSS   + AA  +W STTSVS SGKRIQREM+E N+DPP  CSAGPKGDNLYHW
Sbjct: 3   IGGGRSSSSSTTATAAGGTWVSTTSVSTSGKRIQREMSELNVDPPPHCSAGPKGDNLYHW 62

Query: 74  FATIFGPSGTPYEGGIFFLDIIFPSDYPFNPPKVVFKTRIYHCNVDSTGNVSLDILKDNW 133
            ATI GP GTPY+GGIFFLDI FPSDYPF PPKVVFKTRIYHCNVD+ GN+SLDILKD+W
Sbjct: 63  VATIIGPPGTPYQGGIFFLDITFPSDYPFKPPKVVFKTRIYHCNVDAAGNLSLDILKDSW 122

Query: 134 SPALTIAKVLIAVKTIFANPNPYDPNAPGVAHLYLADRAKHDEIAAEWTLRFAK 186
           SPALT+ KVL A+K+IF NP+PY+P+  G+A LYL D+AKHDEIAAEWTLRFAK
Sbjct: 123 SPALTVTKVLTAIKSIFTNPDPYNPHVSGIARLYLTDKAKHDEIAAEWTLRFAK 176

BLAST of CSPI04G12950.1 vs. NCBI nr
Match: gi|566148311|ref|XP_002299458.2| (hypothetical protein POPTR_0001s10830g [Populus trichocarpa])

HSP 1 Score: 285.4 bits (729), Expect = 7.0e-74
Identity = 130/171 (76.02%), Postives = 150/171 (87.72%), Query Frame = 1

Query: 15  GGGRSSSSISLAAPTSWASTTSVSASGKRIQREMTEFNLDPPSDCSAGPKGDNLYHWFAT 74
           GGG SS++    A +SW S +SVS SGKRIQREMTE N+DPP DCSAGPKGDNLYHW +T
Sbjct: 11  GGGGSSAT----ATSSWVSPSSVSTSGKRIQREMTELNMDPPPDCSAGPKGDNLYHWIST 70

Query: 75  IFGPSGTPYEGGIFFLDIIFPSDYPFNPPKVVFKTRIYHCNVDSTGNVSLDILKDNWSPA 134
           + GP GTPY+GGIFFLD+ FPSDYPF PPKVVFKTRIYHCNVDS GN+SLDILKD+WSPA
Sbjct: 71  VIGPPGTPYQGGIFFLDLTFPSDYPFKPPKVVFKTRIYHCNVDSAGNLSLDILKDSWSPA 130

Query: 135 LTIAKVLIAVKTIFANPNPYDPNAPGVAHLYLADRAKHDEIAAEWTLRFAK 186
           LTI KVL+A+++IF +P+PY+P  PG+AHLYLADRAKHDE+AAEWTLRFAK
Sbjct: 131 LTITKVLLAIRSIFTHPDPYNPLVPGIAHLYLADRAKHDELAAEWTLRFAK 177

BLAST of CSPI04G12950.1 vs. NCBI nr
Match: gi|729348122|ref|XP_010542463.1| (PREDICTED: constitutive photomorphogenesis protein 10 [Tarenaya hassleriana])

HSP 1 Score: 283.5 bits (724), Expect = 2.7e-73
Identity = 131/181 (72.38%), Postives = 151/181 (83.43%), Query Frame = 1

Query: 11  LPPLGGGRSSSSISL------AAPTSWASTTSVSASGKRIQREMTEFNLDPPSDCSAGPK 70
           +PP GGGR +S  +          +SW S TSVSASGKRIQREMTE N+DPP DCSAGPK
Sbjct: 2   MPPGGGGRLTSFPAAMYGGGTGTTSSWVSKTSVSASGKRIQREMTELNMDPPPDCSAGPK 61

Query: 71  GDNLYHWFATIFGPSGTPYEGGIFFLDIIFPSDYPFNPPKVVFKTRIYHCNVDSTGNVSL 130
           GDNLYHW ATI GP GTPYEGGIFF+DIIFPSDYPF PPKVVFKTRIYHCNVD++GN+SL
Sbjct: 62  GDNLYHWIATIIGPEGTPYEGGIFFMDIIFPSDYPFKPPKVVFKTRIYHCNVDTSGNLSL 121

Query: 131 DILKDNWSPALTIAKVLIAVKTIFANPNPYDPNAPGVAHLYLADRAKHDEIAAEWTLRFA 186
           DIL+DNWSPA TI K+L A+++ F  P+PY+P  PG+AHLYL+DRAKHDE+AAEWTLRFA
Sbjct: 122 DILRDNWSPARTITKILQAIRSNFIKPDPYNPALPGIAHLYLSDRAKHDELAAEWTLRFA 181

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
COP10_ARATH3.6e-7075.80Constitutive photomorphogenesis protein 10 OS=Arabidopsis thaliana GN=COP10 PE=1... [more]
UB2E3_MOUSE9.0e-5354.95Ubiquitin-conjugating enzyme E2 E3 OS=Mus musculus GN=Ube2e3 PE=1 SV=2[more]
UB2E3_HUMAN9.0e-5354.95Ubiquitin-conjugating enzyme E2 E3 OS=Homo sapiens GN=UBE2E3 PE=1 SV=1[more]
UB2E3_BOVIN9.0e-5354.95Ubiquitin-conjugating enzyme E2 E3 OS=Bos taurus GN=UBE2E3 PE=2 SV=1[more]
UB2E2_MOUSE2.2e-5157.89Ubiquitin-conjugating enzyme E2 E2 OS=Mus musculus GN=Ube2e2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KWU2_CUCSA1.6e-10499.46Uncharacterized protein OS=Cucumis sativus GN=Csa_4G280420 PE=4 SV=1[more]
A0A061GS19_THECC3.8e-7476.44Ubiquitin-conjugating enzyme family protein isoform 1 OS=Theobroma cacao GN=TCM_... [more]
B9GL37_POPTR4.9e-7476.02Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0001s10830g PE=4 SV=2[more]
B9SM61_RICCO2.4e-7374.27Ubiquitin-conjugating enzyme E2, putative OS=Ricinus communis GN=RCOM_0878930 PE... [more]
A0A0D2R6Z2_GOSRA9.3e-7376.44Uncharacterized protein OS=Gossypium raimondii GN=B456_010G126700 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT3G13550.12.0e-7175.80 Ubiquitin-conjugating enzyme family protein[more]
AT2G16740.11.2e-4752.45 ubiquitin-conjugating enzyme 29[more]
AT3G08690.11.3e-4651.72 ubiquitin-conjugating enzyme 11[more]
AT5G56150.18.6e-4649.66 ubiquitin-conjugating enzyme 30[more]
AT4G27960.21.9e-4549.66 ubiquitin conjugating enzyme 9[more]
Match NameE-valueIdentityDescription
gi|449448836|ref|XP_004142171.1|2.2e-10499.46PREDICTED: constitutive photomorphogenesis protein 10 [Cucumis sativus][more]
gi|659097798|ref|XP_008449820.1|4.7e-10297.84PREDICTED: constitutive photomorphogenesis protein 10 [Cucumis melo][more]
gi|590582669|ref|XP_007014687.1|5.4e-7476.44Ubiquitin-conjugating enzyme family protein isoform 1 [Theobroma cacao][more]
gi|566148311|ref|XP_002299458.2|7.0e-7476.02hypothetical protein POPTR_0001s10830g [Populus trichocarpa][more]
gi|729348122|ref|XP_010542463.1|2.7e-7372.38PREDICTED: constitutive photomorphogenesis protein 10 [Tarenaya hassleriana][more]
The following terms have been associated with this mRNA:
Vocabulary: INTERPRO
TermDefinition
IPR000608UBQ-conjugat_E2
IPR016135UBQ-conjugating_enzyme/RWD
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042023 DNA endoreduplication
biological_process GO:0043248 proteasome assembly
biological_process GO:0043161 proteasome-mediated ubiquitin-dependent protein catabolic process
biological_process GO:0000338 protein deneddylation
biological_process GO:0016567 protein ubiquitination
biological_process GO:0010099 regulation of photomorphogenesis
biological_process GO:0051510 regulation of unidimensional cell growth
biological_process GO:0051788 response to misfolded protein
cellular_component GO:0005634 nucleus
molecular_function GO:0016874 ligase activity
molecular_function GO:0004842 ubiquitin-protein transferase activity

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
CSPI04G12950CSPI04G12950gene


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
CSPI04G12950.1CSPI04G12950.1-proteinpolypeptide


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
CSPI04G12950.1.utr5p1CSPI04G12950.1.utr5p1five_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
CSPI04G12950.1.cds1CSPI04G12950.1.cds1CDS
CSPI04G12950.1.cds2CSPI04G12950.1.cds2CDS
CSPI04G12950.1.cds3CSPI04G12950.1.cds3CDS
CSPI04G12950.1.cds4CSPI04G12950.1.cds4CDS


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
CSPI04G12950.1.utr3p1CSPI04G12950.1.utr3p1three_prime_UTR


Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000608Ubiquitin-conjugating enzyme E2PFAMPF00179UQ_concoord: 43..178
score: 1.5
IPR000608Ubiquitin-conjugating enzyme E2PROFILEPS50127UBIQUITIN_CONJUGAT_2coord: 42..174
score: 38
IPR016135Ubiquitin-conjugating enzyme/RWD-likeGENE3DG3DSA:3.10.110.10coord: 30..184
score: 3.7
IPR016135Ubiquitin-conjugating enzyme/RWD-likeunknownSSF54495UBC-likecoord: 28..184
score: 1.97
NoneNo IPR availablePANTHERPTHR24068FAMILY NOT NAMEDcoord: 34..185
score: 1.3
NoneNo IPR availableSMARTSM00212ubc_7coord: 42..185
score: 3.3